Publications by authors named "Zhengwen Yu"

7 Publications

  • Page 1 of 1

The complete chloroplast genome of Hsu et S. C. Cheng and its phylogenetic analysis.

Mitochondrial DNA B Resour 2021 Mar 15;6(3):842-843. Epub 2021 Mar 15.

School of Life Science, Guizhou Normal University, Guiyang, China.

Hsu et S. C. Cheng is widely used as an edible and medicinal food in China and also displays excellent pharmacological activities. The phylogenetic relationship between and other family members remains unclear. In this work, we assembled the cp genome of using the high-throughput Illumina pair-end sequencing data. The circular cp genome is 155,102 bp in size, including a large single-copy (LSC) region of 88,906 bp and a small single-copy (SSC) region of 18,628 bp, which were separated by two inverted repeat (IR) regions (23,784 bp each). A total of 129 genes were predicted, including eight ribosomal RNAs (rRNAs), 39 transfer RNAs (tRNAs), and 82 protein-coding genes (PCGs). Furthermore, phylogenetic analysis revealed that formed a different clade from other two congeneric species ( and ). This study provides useful information for future genetic study of .
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http://dx.doi.org/10.1080/23802359.2021.1884027DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7971229PMC
March 2021

The complete chloroplast genome of Miq (Caprifoliaceae: Dipsacales) from Guangxi, China.

Mitochondrial DNA B Resour 2021 Feb 9;6(2):450-451. Epub 2021 Feb 9.

School of Life Science, Guizhou Normal University, Guiyang, China.

Miq, which is widely distribute in south China, is an important Chinese plant used in traditional medicine. Here we report the first complete chloroplast (cp) genome sequence of this species. The circular cp genome is 154,581 bp in size, including a large single-copy (LSC) region of 88,379 bp and a small single-copy (SSC) region of 18,646 bp, which were separated by two inverted repeat (IR) regions (IRA and B, 23,778 bp each). A total of 121 genes were annotated, including 8 ribosomal RNAs (rRNAs), 33 transfer RNAs (tRNAs) and 80 protein-coding genes (PCGs). Phylogenetic analysis of 20 representative members within the Caprifoliaceae showed that is closely related to the . This study provides important genetic information for future systematic and evolutionary studies of .
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http://dx.doi.org/10.1080/23802359.2020.1870903DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7872560PMC
February 2021

Electroacupuncture reduces scopolamine-induced amnesia via mediating the miR-210/SIN3A and miR-183/SIN3A signaling pathway.

Mol Med 2020 11 12;26(1):107. Epub 2020 Nov 12.

Department of Anesthesiology, Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Arts and Science, No.136, Jingzhou Street, Xiangcheng District, Xiangyang, 441021, Hubei, China.

Background: The expression of SIN3A is closely correlated with electroacupuncture (EA) treatment efficacy of scopolamine-induced amnesia (SIA), but its underlying mechanisms remain to be further explored.

Methods: Quantitative real-time PCR was performed to analyze the expression of candidate microRNAs (miRNAs) and SIN3A mRNA in a rat model of SIA. Western blot was carried out to evaluate the differential expression of SIN3A proteins under different circumstances. Luciferase assay was used to explore the inhibitory role of certain miRNAs in SIN3A expression. A novel object recognition (NOR) test was performed to assess the memory function of SIA rats undergoing EA treatment. Immunohistochemistry was carried out to evaluate the expression of SIN3A in the hippocampus of SIA rats.

Results: Rno-miR-183-5p, rno-miR-34c-3p and rno-miR-210-3p were significantly up-regulated in SIA rats treated with EA. In addition, rno-miR-183-5p and rno-miR-210-3p exerted an inhibitory effect on SIN3A expression. EA treatment of SIA rats effectively restored the dysregulated expression of rno-miR-183-5p, rno-miR-210-3p and SIN3A. EA treatment also promoted the inhibited expression of neuronal IEGs including Arc, Egr1, Homer1 and Narp in the hippocampus of SIA rats. Accordingly, the NOR test also confirmed the effect of EA treatment on the improvement of memory in SIA rats.

Conclusion: In summary, the findings of this study demonstrated that scopolamine-induced amnesia was associated with downregulated expression of miR-210/miR-183 and upregulated expression of SIN3A. Furthermore, treatment with EA alleviated scopolamine-induced amnesia in rats and was associated with upregulated expression of miR-210/miR-183 and downregulated expression of SIN3A.
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http://dx.doi.org/10.1186/s10020-020-00233-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7661264PMC
November 2020

Chemical Compositionand Anti-acetyl cholinesterase Activity of Flower Essential Oils of Artemisiaannuaat Different Flowering Stage.

Iran J Pharm Res 2011 ;10(2):265-71

Bioengineering College of Chongqing University and Drug Screening Lab, The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Science. ; School of Life Sciences of Guizhou Normal University, Guiyang, Guizhou, 550001, P.R.China.

The chemical composition of the essential oils of flower at the pre-flowering, full-flowering and post-flowering stage of A. annua was analyzed by GC and GC/MS and sixty-two components were identified. The main compounds in the pre-flowering oil were β-myrcene (37.71%), 1, 8-cineole (16.11%) and camphor (14.97%). The full-flowering oil contained predominantly caryophyllene (19.4%), germacrene D (18.1%), camphor (15.84%), 1, 8-cineole (10.6%) and (Z)-β-farnesene (9.43%). The major constituents identified in the post-flowering oil were camphor (16.62%), caryophyllene (16.27%), β-caryophyllene oxide (15.84%), β-farnesene (9.05%) and (-)-spathulenol (7.21%). The variety of anti-AChE activity of flower oil of A. annua at three flowering stage might be a result of the variety of the content and interaction of those terpenoids with anti-AChE activity. The greatest acetylcholinesterase inhibitory activity (IC50 = 0.13 ± 0.02 mg mL(-1)) was exhibited by the essential oil of flower of A. annua at post-flowering stage.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3828900PMC
November 2013

Screening the Hemostatic Active Fraction of Artemisia annua L. In-vitro.

Iran J Pharm Res 2011 ;10(1):57-62

College of Bioengineering, Chongqing University, Chongqing, 400030, P.R.China.

Artemisinin extracted from Artemisia annua L. is the best medicine with the highest efficiency, the most effective and the lowest toxicity in treating ague nowadays. At present, most studies focus on artemisinin and its derivatives, while the study and report about the other active components are rare. This paper purposed to further discover new indication of Artemisia annua L. connecting with the record of traditional medicine. We screened the hemostatic active fraction of Artemisia annua L. in-vitro by plasma recalcification time (PRT). The crude extract and the extract of n-butanol were purified by polyamide, MCI, gel column in order. Determining the part of 20% methanol fraction after column chromatography of MCI gel is the hemostatic active fraction of Artemisia annua L. The shorten rate of clotting time are followed by: crude extract of Artemisin annua L. (8.51%); the n-butanol extract (14.89%); water eluting fraction after the extract of n-butanol was purificated by polyamide (22.11%); 20% methanol fraction after column chromatography of MCI gel (27.37%). It can provide experimental data for the clinical application of Artemisia annua L. which can be exploited as hemostatic. This topic has a certain academic value and potential prospects on the deep research of the Artemisia annua L. resource.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3869591PMC
December 2013

A novel hemostatic model with triple protective functions.

Med Hypotheses 2009 Feb 11;72(2):186-7. Epub 2008 Oct 11.

College of Bioengineering, Chongqing University, Chongqing 400030, People's Republic of China.

While the role of hemostatic activity is well established, the importance of infection prevention in the wound has not been addressed. Based on the available evidence from disinfection and infection prevention, essential oils are attractive candidates for antimicrobial drugs. Therefore, we hypothesize to design a novel hemostatic model with triple protective functions through coordinating essential oils to hematostatic ingredients. The hematostatic ingredients play their roles in hemostasis when hemorrhage occurs. Simultaneously, the essential oils evaporate naturally to form a protective layer around the wound, which can help to prevent pathogenic bacteria from infecting the wound. What more, most plant essential oils possess antibacterial activity. Thus, the essential oils can be used as an accessory treatment for infection once the wound infected by pathogenic bacteria. So the novel hematostatic model with triple protective functions will have broader utility in therapy of out-wound bleeding than current hemostatic drugs.
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http://dx.doi.org/10.1016/j.mehy.2008.09.004DOI Listing
February 2009

A new probe for targeting drug delivery system.

Med Hypotheses 2009 Jan 30;72(1):43-4. Epub 2008 Sep 30.

College of Bioengineering, Chongqing University, Chongqing 400030, China.

Recently, TDDS (Targeting drug delivery system) plays an important role in enhancing the bioavailability and targeting of anti-tumor drugs. How to transport drugs quickly and precisely to their target sites of action has not been solved fundamentally. A large number of researches have identified artemisinin and its analogs have the merit of precisely targeting to cancer cell, and low side effects to healthy tissue. Thus, if these compounds could be attached to established anti-tumor drugs with probe, a novel targeting anti-tumor drugs will be put into practice in the future. The novel drugs delivery system will be a powerful weapon against cancer disease for their unique targeting.
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http://dx.doi.org/10.1016/j.mehy.2008.07.057DOI Listing
January 2009