Publications by authors named "Zheng Wang"

2,813 Publications

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Associations of affective and cognitive empathy with depressive symptoms among a sample of Chinese college freshmen.

J Affect Disord 2021 Jun 4;292:652-659. Epub 2021 Jun 4.

Research Center for Psychology and Behavioral Sciences, Soochow University, Suzhou, China; School of Public Health, Jiangsu Key Laboratory of Preventive and Translational Medicine for Geriatric Diseases, Medical College of Soochow University, Suzhou, China. Electronic address:

Background: College freshmen are at high risk for mental and socioemotional problems after entering a new environment. However, few investigators have evaluated the associations between empathy and depressive symptoms among college freshmen. The present study examined the presence and associations of affective and cognitive empathy with depressive symptoms among college freshmen in China.

Methods: In total, 4297 college freshmen completed the Interpersonal Reactivity Index as an assessment of empathy and the University Personality Inventory to evaluate the prevalence of depressive symptoms.

Results: The empathy scores of females were higher than that of males, and approximately 18.4% of freshmen had high-depressive symptoms (HDS). Freshmen with a higher positive component of affective empathy (empathic concern (EC)) experienced fewer HDS. In contrast, freshmen with a higher negative component of affective empathy (personal distress (PD)) and lower cognitive empathy (perspective taking (PT)) experienced more HDS. EC was negatively associated with depressive symptoms, PD was positively related to depressive symptoms, and PT was negatively associated with depressive symptoms. Males with a higher degree of PT experienced fewer HDS, while females with a higher degree of PT experienced more HDS.

Conclusion: Affective empathy was positively associated with depressive symptoms, while cognitive empathy was negatively related to depressive symptoms. The association between affective and cognitive empathy with depressive symptoms may add some support to the detection of clinical depressive symptoms. These findings call for the necessity of considering the characteristics of affective and cognitive empathy as a crucial concern in the prevention of depressive symptoms.
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http://dx.doi.org/10.1016/j.jad.2021.05.111DOI Listing
June 2021

Production of 3-hydroxypropionic acid from glucose and xylose by metabolically engineered .

Metab Eng Commun 2015 Dec 31;2:132-136. Epub 2015 Oct 31.

The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kogle Allé 6, 2970 Hørsholm, Denmark.

Biomass, the most abundant carbon source on the planet, may in the future become the primary feedstock for production of fuels and chemicals, replacing fossil feedstocks. This will, however, require development of cell factories that can convert both C6 and C5 sugars present in lignocellulosic biomass into the products of interest. We engineered for production of 3-hydroxypropionic acid (3HP), a potential building block for acrylates, from glucose and xylose. We introduced the 3HP biosynthetic pathways via malonyl-CoA or β-alanine intermediates into a xylose-consuming yeast. Using controlled fed-batch cultivation, we obtained 7.37±0.17 g 3HP L in 120 hours with an overall yield of 29±1% Cmol 3HP Cmol xylose. This study is the first demonstration of the potential of using for production of 3HP from the biomass sugar xylose.
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http://dx.doi.org/10.1016/j.meteno.2015.10.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193239PMC
December 2015

Photoelectric scanning-based resection method for mobile robot localization.

Appl Opt 2021 Jun;60(17):5020-5030

Indoor localization is a key enabling technology for mobile robot navigation in industrial manufacturing. As a distributed metrology system based on multi-station intersection measurement, the workshop measurement positioning system (wMPS) is gaining increasing attention in mobile robot localization. In this paper, a new, to the best of our knowledge, wMPS-based resection localization method is proposed using a single onmidirectional transmitter mounted on a mobile robot with scanning photoelectric receivers distributed in the work space. Compared to the traditional method that requires multiple stationary transmitters, our new method provides higher flexibility and cost-effectiveness. The position and orientation of the mobile robot are then iteratively optimized with respect to the constraint equations. In order to obtain the optimal solution rapidly, two methods of initial value determination are presented for different numbers of effective receivers. The propagation of the localization uncertainty is also investigated using Monte-Carlo simulations. Moreover, two experiments of automated guided vehicle localization are conducted, and the results demonstrate the high accuracy of the proposed method.
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http://dx.doi.org/10.1364/AO.422152DOI Listing
June 2021

Matrix stiffness and its influence on pancreatic diseases.

Biochim Biophys Acta Rev Cancer 2021 Jun 14:188583. Epub 2021 Jun 14.

Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, 710061 Xi'an, Shaanxi Province, China. Electronic address:

The matrix stiffness of the extracellular matrix(ECM), which is the slow elastic force on cells, has gradually become investigated. And a higher stiffness could induce changes in cell biological behaviors and activation of internal signaling pathways. Imbalanced stiffness of ECM is associated with a number of diseases, including pancreatic disease. In this review, we discuss the components of the ECM and the increased stiffness caused by unbalanced ECM changes. Next, we describe how matrix stiffness transmits mechanical signals and what signaling pathways are altered within the cell in detail. Finally, we discuss the effect of ECM on the behavior of pancreatic diseases from the perspective of matrix stiffness.
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http://dx.doi.org/10.1016/j.bbcan.2021.188583DOI Listing
June 2021

Strontium-calcium phosphate hybrid cement with enhanced osteogenic and angiogenic properties for vascularised bone regeneration.

J Mater Chem B 2021 Jun 17. Epub 2021 Jun 17.

Institute of Orthopedics and Department of Orthopedics, The First Affiliated Hospital, Soochow University, No. 708 Renmin Road, Suzhou 215006, P. R. China and Center for Health Science and Engineering (CHSE), School of Materials Science and Engineering, Hebei University of Technology, No. 8 Guangrong Road, Tianjin 300130, P. R. China.

Vascularized bone tissue engineering is regarded as one of the optimal treatment options for large bone defects. The lack of angiogenic properties and unsatisfactory physicochemical performance restricts calcium phosphate cement (CPC) from application in vascularized bone tissue engineering. Our previous studies have developed a starch and BaSO4 incorporated calcium phosphate hybrid cement (CPHC) with improved mechanical strength and handling properties. However, the bioactivity-especially the angiogenic ability-is still absent and requires further improvement. Herein, based on the reported CPHC and the osteogenic and angiogenic properties of strontium (Sr) ions, a strontium-enhanced calcium phosphate hybrid cement (Sr-CPHC) was developed to improve both biological and physicochemical properties of CPC. Compared to CPC, the initial setting time of Sr-CPHC was prolonged from 2.2 min to 20.7 min. The compressive strength of Sr-CPHC improved from 11.21 MPa to 45.52 MPa compared with CPC as well. Sr-CPHC was biocompatible and showed promotion of alkaline phosphatase (ALP) activity, calcium nodule formation and osteogenic relative gene expression, suggesting high osteogenic-inductivity. Sr-CPHC also facilitated the migration and tube formation of human umbilical vein endothelial cells (HUVECs) in vitro and up-regulated the expression of the vascular endothelial growth factor (VEGF) and Angiopoietin-1 (Ang-1). In vivo evaluation showed marked new bone formation in a rat calvarial defect model with Sr-CPHC implanted. Sr-CPHC also exhibited enhancement of neovascularization in subcutaneous connective tissue in a rat subcutaneous implantation model. Thus, the Sr-CPHC with the dual effects of osteogenesis and angiogenesis shows great potential for clinical applications such as the repair of ischemic osteonecrosis and critical-size bone defects.
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http://dx.doi.org/10.1039/d1tb00439eDOI Listing
June 2021

Systematic analysis of lysine 2-hydroxyisobutyrylation posttranslational modification in wheat leaves.

PLoS One 2021 17;16(6):e0253325. Epub 2021 Jun 17.

Crop Research Institute, Shandong Academy of Agricultural Sciences, Ji'nan, Shandong, P. R. China.

Lysine 2-hydroxyisobutyrylation (Khib) is a recently discovered post-translational modification (PTM) showing diverse biological functions and effects in living organisms. However, the study of Khib in plant species is still relatively limited. Wheat (Triticum aestivum L.) is a global important cereal plant. In this study, the systematic Khib analysis was performed in wheat leave tissues. A total of 3004 Khib sites in 1104 proteins were repeatedly identified. Structure characterization of these Khib peptides revealed 12 conserved sequence motifs. Function classification and enrichment analysis indicated these Khib proteins showed a wide function and pathway distribution, of which ribosome activity, protein biosynthesis and photosynthesis were the preferred biological processes. Subcellular location predication indicated chloroplast was the dominant subcellular compartment where Khib was distributed. There may be some crosstalks among Khib, lysine acetylation and lysine succinylation modification because some proteins and sites were modified by all these three acylations. The present study demonstrated the critical role of Khib in wheat biological and physiology, which has expanded the scope of Khib in plant species. Our study is an available resource and reference of Khib function demonstration and structure characterization in cereal plant, as well as in plant kingdom.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0253325PLOS
June 2021

Extraction of Preisach model parameters for fluorite-structure ferroelectrics and antiferroelectrics.

Sci Rep 2021 Jun 14;11(1):12474. Epub 2021 Jun 14.

Georgia Institute of Technology, School of Electrical and Computer Engineering, Atlanta, 30332, USA.

Flourite-structure ferroelectrics (FEs) and antiferroelectrics (AFEs) such as HfO and its variants have gained copious attention from the semiconductor community, because they enable complementary metal-oxide-semiconductor (CMOS)-compatible platforms for high-density, high-performance non-volatile and volatile memory technologies. While many individual experiments have been conducted to characterize and understand fluorite-structure FEs and AFEs, there has been little effort to aggregate the information needed to benchmark and provide insights into their properties. We present a fast and robust modeling framework that automatically fits the Preisach model to the experimental polarization ([Formula: see text]) versus electric field ([Formula: see text]) hysteresis characterizations of fluorite-structure FEs. The modifications to the original Preisach model allow the double hysteresis loops in fluorite-structure antiferroelectrics to be captured as well. By fitting the measured data reported in the literature, we observe that ferroelectric polarization and dielectric constant decrease as the coercive field rises in general.
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http://dx.doi.org/10.1038/s41598-021-91492-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8203701PMC
June 2021

Host and gut microbial tryptophan metabolism and type 2 diabetes: an integrative analysis of host genetics, diet, gut microbiome and circulating metabolites in cohort studies.

Gut 2021 Jun 14. Epub 2021 Jun 14.

Department of Epidemiology, Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland, USA.

Objective: Tryptophan can be catabolised to various metabolites through host kynurenine and microbial indole pathways. We aimed to examine relationships of host and microbial tryptophan metabolites with incident type 2 diabetes (T2D), host genetics, diet and gut microbiota.

Method: We analysed associations between circulating levels of 11 tryptophan metabolites and incident T2D in 9180 participants of diverse racial/ethnic backgrounds from five cohorts. We examined host genome-wide variants, dietary intake and gut microbiome associated with these metabolites.

Results: Tryptophan, four kynurenine-pathway metabolites (kynurenine, kynurenate, xanthurenate and quinolinate) and indolelactate were positively associated with T2D risk, while indolepropionate was inversely associated with T2D risk. We identified multiple host genetic variants, dietary factors, gut bacteria and their potential interplay associated with these T2D-relaetd metabolites. Intakes of fibre-rich foods, but not protein/tryptophan-rich foods, were the dietary factors most strongly associated with tryptophan metabolites. The fibre-indolepropionate association was partially explained by indolepropionate-associated gut bacteria, mostly fibre-using . We identified a novel association between a host functional variant (determining lactase persistence) and serum indolepropionate, which might be related to a host gene-diet interaction on gut , a probiotic bacterium significantly associated with indolepropionate independent of other fibre-related bacteria. Higher milk intake was associated with higher levels of gut and serum indolepropionate only among genetically lactase non-persistent individuals.

Conclusion: Higher milk intake among lactase non-persistent individuals, and higher fibre intake were associated with a favourable profile of circulating tryptophan metabolites for T2D, potentially through the host-microbial cross-talk shifting tryptophan metabolism toward gut microbial indolepropionate production.
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http://dx.doi.org/10.1136/gutjnl-2021-324053DOI Listing
June 2021

Evolution and challenges of DNS-based CDNs.

Digit Commun Netw 2018 ;4(4)

Advanced Network Technologies Division, National Institute of Standards and Technology, USA.

DNS-based server redirecting is considered the most popular means of deploying CDNs. However, with the increasing use of remote DNS, DNS-based CDNs face a great challenge in performance degradation. To address this issue, encouraging progress has been made in both industry and research communities. In this article, state-of-art solutions for the remote DNS problem are discussed at first. Next, privacy concerns about DNS-based CDNs, including client location as well as redirection privacy, are identified and a representative solution is summarized. Finally, the solution is compared to those in prior works under different measures, and a discussion on DNS-based CDN applications is provided. A model is also established to deepen the understanding of CDN performance. We believe that this survey will shed light on the application of DNS-based CDNs, and it is expected to provide design guidelines to CDN service providers.
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http://dx.doi.org/10.1016/j.dcan.2017.07.005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8191411PMC
January 2018

Energy-aware DNS server allocating.

Wirel Commun Mob Comput 2018 Sep;19

National Institute of Standards and Technology, Gaithersburg MD 20899, USA.

Faced with the scalability and reliability challenge, the DNS is increasingly operated by geographically dispersed data centers. Energy management across those distributed diverse data centers is critical to reducing revenue loss for DNS operators. This paper addresses the DNS server allocating problem aiming at optimizing revenue loss in terms of delay cost and energy cost. An analytical model is proposed taking network delay, query processing delay, energy costs, and server selections into consideration. The optimal solution is obtained analytically given static parameters or using an iteration method given dynamic parameters. The effectiveness of energy-aware DNS server allocating and the feasibility of the proposed iteration method are validated through simulations.
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http://dx.doi.org/10.1016/j.suscom.2018.07.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8191410PMC
September 2018

Deep learning for tracing esophageal motility function over time.

Comput Methods Programs Biomed 2021 Jun 1;207:106212. Epub 2021 Jun 1.

Department of Gastroenterology of Xiangya hospital, Central South University, Changsha 410008, China. Electronic address:

Background And Objective: Esophageal high-resolution manometry (HRM) is widely performed to evaluate the representation of manometric features in patients for diagnosing normal esophageal motility and motility disorders. Clinicians commonly assess esophageal motility function using a scheme termed the Chicago classification, which is difficult, time-consuming and inefficient with large amounts of data.

Methods: Deep learning is a promising approach for diagnosing disorders and has various attractive advantages. In this study, we effectively trace esophageal motility function with HRM by using a deep learning computational model, namely, EMD-DL, which leverages three-dimensional convolution (Conv3D) and bidirectional convolutional long-short-term-memory (BiConvLSTM) models. More specifically, to fully exploit wet swallowing information, we establish an efficient swallowing representation method by localizing manometric features and swallowing box regressions from HRM. Then, EMD-DL learns how to identify major motility disorders, minor motility disorders and normal motility. To the best of our knowledge, this is the first attempt to use Conv3D and BiConvLSTM to predict esophageal motility function over esophageal HRM.

Results: Test experiments on HRM datasets demonstrated that the overall accuracy of the proposed EMD-DL model is 91.32% with 90.5% sensitivity and 95.87% specificity. By leveraging information across swallowing motor cycles, our model can rapidly recognize esophageal motility function better than a gastroenterologist and lays the foundation for accurately diagnosing esophageal motility disorders in real time.

Conclusions: This approach opens new avenues for detecting and identifying esophageal motility function, thereby facilitating more efficient computer-aided diagnosis in clinical practice.
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http://dx.doi.org/10.1016/j.cmpb.2021.106212DOI Listing
June 2021

Spino cranial angle as a predictor of loss of cervical lordosis after laminoplasty in patients with cervical myelopathy.

BMC Surg 2021 Jun 12;21(1):291. Epub 2021 Jun 12.

Department of Spinal Surgery, The Third Hospital of Hebei Medical University, 139 Ziqiang Road, Shijiazhuang, 050051, People's Republic of China.

Background: To explore the relationship between spino cranial angle (SCA) and loss of cervical lordosis (LOCL), and to determine whether SCA has the ability to predict LOCL for patients with cervical myelopathy.

Methods: A total of 68 consecutive patients with cervical myelopathy who received laminoplasty (LAMP) were selected to the current study. C2-C7 lordosis was defined as a representation of the cervical alignment. Alignment change > 0° was considered LOCL. Multiple linear regression analysis was applied to evaluate the association between LOCL and various sagittal parameters at preoperative, such as SCA, CL, T1s and cSVA. Linear regression analysis was applied to evaluate the relationships between LOCL and preoperative SCA in each subgroup.

Results: Patients were assigned to three groups depending on the quartile of preoperative SCA. The first quarter of patients were defined as the low SCA group, the last quarter were defined as the high SCA group and the middle half were defined as the middle SCA group. There was no statistically significant difference in age, sex and the type of OPLL among the three groups. Patients in the low SCA group showed more cervical lordosis before surgery and more LOCL after LAMP (p < 0.001). After linear regression analysis for SCA and LOCL, preoperative SCA was negatively correlated with LOCL in the low SCA group (r = - 0.857, p < 0.001) and high SCA group (r = - 0.515, p = 0.034). However, there was no significant correlation between preoperative SCA and LOCL in the middle SCA group (r = 0.027, p = 0.881).

Conclusions: Patients with lower SCA had more lordosis preoperatively and performed more LOCL after LAMP at 2 years of follow-up. Both too high or low preoperative SCA were negatively correlated with the degree of LOCL, while when the SCA fluctuates in a suitable range, it is easier to compensate for the changes of cervical sagittal alignment.
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http://dx.doi.org/10.1186/s12893-021-01293-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8199803PMC
June 2021

Deactivation of Caenorhabditis elegans nematodes in drinking water by PMS/UV-C: efficiency and mechanisms.

Environ Sci Pollut Res Int 2021 Jun 12. Epub 2021 Jun 12.

State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai, 200092, China.

The occurrence and infestations of chlorine-resistant invertebrates in drinking water distributions have attracted concerns on water quality in China, making effective deactivation imperative. This study presents a novel strategy for nematode (Caenorhabditis elegans) deactivation using peroxymonosulfate (PMS)/UV-C. The results indicated that 100% deactivation efficiency was obtained under optimal conditions. An acidic pH and 0.25 mg/L Fe(II) were beneficial to the PMS/UV-C-triggered deactivation of nematodes. A mechanism study demonstrated that [Formula: see text] was activated by UV-C to produce ·OH and [Formula: see text], which resulted in oxidative stress and stimulated the occurrence of cell apoptosis, leading to nematode deactivation. The results reveal PMS/UV-C as an alternative to chlorination in water treatment plants (WTP) or an emergency application when chlorine-resistant invertebrates breed in a second-supply water tank is a promising strategy for disinfection. This approach possessed the advantages of avoiding the production of chlorine disinfection by-products (DBP) and greater efficacy of nematode deactivation. This work will provide ideas for on-going research efforts into chlorine-resistant invertebrate deactivation and eventually achieve the direct drinking of municipal tap water.
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http://dx.doi.org/10.1007/s11356-021-14312-8DOI Listing
June 2021

SIRT1/PGC-1α signaling activation by mangiferin attenuates cerebral hypoxia/reoxygenation injury in neuroblastoma cells.

Eur J Pharmacol 2021 Jun 8:174236. Epub 2021 Jun 8.

Xi'an Key Laboratory of Cardiovascular and Cerebrovascular Diseases, Xi'an No.3 Hospital, School of Life Sciences and Medicine, Northwest University, 10 Fengcheng Three Road, Xi'an, China; Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education. Faculty of Life Sciences, Northwest University, 229 Taibai North Road, Xi'an, China. Electronic address:

Ischemia reperfusion injury (IRI) is associated with poor prognoses in the setting of ischemic brain diseases. Silence information regulator 1 (SIRT1) is a member of the third class of nicotinamide adenine dinucleotide (NAD)-dependent sirtuins. Recently, the role of SIRT1/peroxisome proliferators-activated receptor-γ coactivator 1α (PGC-1α) pathway in organ (especially the brain) protection under various pathological conditions has been widely investigated. Mangiferin (MGF), a natural C-glucosyl xanthone polyhydroxy polyphenol, has been shown to be beneficial to several nervous system diseases and the protective effects of MGF can be achieved through the regulation of SIRT1 signaling. This study is designed to investigate the protective effect of MGF treatment in the setting of cerebral IRI and to elucidate the potential mechanisms. We first evaluated the toxicity of MGF and chose the safe concentrations for the following experiments. MGF exerted obvious neuroprotection against hypoxia/reoxygenation (HR)-induced injury, indicated by restored cell viability and cell morphology, decreased lactate dehydrogenase (LDH) release and reactive oxygen species generation. MGF also restored the protein expressions of SIRT1, PGC-1α, Nrf2, NQO1, HO-1, NRF1, UCP2, and Bcl2 down-regulated by HR treatment. However, SIRT1 siRNA could reverse MGF-induced neuroprotection and decrease the expressions of molecules mentioned above. Taken together, our findings suggest that MGF treatment exerts neuroprotection against HR injury via activating SIRT1/PGC-1α signaling. These findings may provide a theoretical basis for the exploitation of MGF as a potential neuroprotective drug candidate, which may be beneficial for the ischemic stroke patients in clinic.
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http://dx.doi.org/10.1016/j.ejphar.2021.174236DOI Listing
June 2021

Identification of Ten Mitosis Genes Associated with Tamoxifen Resistance in Breast Cancer.

Onco Targets Ther 2021 4;14:3611-3624. Epub 2021 Jun 4.

Department of General Surgery, Comprehensive Breast Health Center, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, People's Republic of China.

Background: Endocrine therapy is the backbone therapy in estrogen receptor α (ER)-positive breast cancer, and tamoxifen resistance is a great challenge for endocrine therapy. Tamoxifen-resistant and sensitive samples from the international public repository, the Gene Expression Omnibus (GEO) database, were used to identify therapeutic biomarkers associated with tamoxifen resistance.

Materials And Methods: In this study, integrated analysis was used to identify tamoxifen resistance-associated genes. Differentially expressed genes (DEGs) were identified. Gene ontology and pathway analysis were then analyzed. Weighted correlation network analysis (WGCNA) was performed to find modules correlated with tamoxifen resistance. Protein-protein interaction (PPI) network was used to find hub genes. Genes of prognostic significance were further validated in another GEO dataset and cohort from Shanghai Ruijin Hospital using RT-PCR.

Results: A total of 441 genes were down-regulated and 123 genes were up-regulated in tamoxifen-resistant samples. Those up-regulated genes were mostly enriched in the cell cycle pathway. Then, WGCNA was performed, and the brown module was correlated with tamoxifen resistance. An overlap of 81 genes was identified between differentially expressed genes (DEGs) and genes in the brown module. These genes were also enriched in the cell cycle. Twelve hub genes were identified using PPI network, which were involved in the mitosis phase of the cell cycle. Finally, 10 of these 12 genes were validated to be up-regulated in tamoxifen-resistant patients and were associated with poor prognosis in ER-positive patients.

Conclusion: Our study suggested mitosis-related genes are mainly involved in tamoxifen resistance, and high expression of these genes could predict poor prognosis of patients receiving tamoxifen. These genes may be potential targets to improve efficacy of endocrine therapy in breast cancer, and inhibitors targeted these genes could be used in endocrine-resistant patients.
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http://dx.doi.org/10.2147/OTT.S290426DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187086PMC
June 2021

Identification and characterization of lncRNA AP000253 in occult hepatitis B virus infection.

Virol J 2021 Jun 10;18(1):125. Epub 2021 Jun 10.

Department of Liver Disease, Wuxi No.5 People's Hospital Affiliated to Jiangnan University, 1215 Guangrui Road, Wuxi, 214000, China.

Background: Recent studies suggest that lncRNAs may play significant roles in the development of hepatitis B virus (HBV) infection. However, as a special stage of HBV infection, the lncRNA expression in occult HBV infection (OBI) remains unclear.

Methods: The plasma level of 15 HBV infection-related lncRNAs was initially detected using qRT-PCR in 10 OBI and 10 healthy controls (HCs) in discovery phase. Significantly dysregulated lncRNAs were subsequently validated in another 64 OBI, 20 HCs, 31 chronic hepatitis B (CHB) and 20 asymptomatic HBsAg carriers (ASC). Moreover, the AP000253 expression in liver tissues and its potential biological functions in HBV infection were further investigate with public transcriptomic data and HBV-expressing cell lines.

Results: Among candidate lncRNAs, the plasma level of AP000253 decreased significantly in OBI, ASC and CHB patients compared to HCs, while no difference was found among OBI, ASC and CHB patients. In liver tissues, similar AP000253 expression was also observed from the GSE83148 dataset, while that in HBV-expressing hepatoma cells was opposite. ROC curve analysis indicated that plasma AP000253 yielded an AUC of 0.73 with 60% sensitivity and 75% specificity when differentiating OBI from HCs, but it could not specifically separate the stage of chronic HBV infection. Furthermore, functional experiments suggested that AP000253 could promote HBV transcription and replication in hepatoma cell lines.

Conclusions: AP000253 might be involved in HBV replication, and be served as a potential biomarker for HBV infection. In the setting of blood donations, plasma AP000253 would be more useful to moderately distinguish OBI in HBsAg-negative donors. However, the AP000253 expression in liver tissues and associated molecular mechanism of HBV infection deserve further study in future.
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http://dx.doi.org/10.1186/s12985-021-01596-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8194241PMC
June 2021

EGFR-mutant NSCLC presenting with stroke and massive systemic embolization as the first manifestation: case report.

BMC Neurol 2021 Jun 9;21(1):221. Epub 2021 Jun 9.

Department of Neurology, The Second Hospital of Hebei Medical University, 309 Zhonghuabei Street, Hebei, 050000, Shijiazhuang, People's Republic of China.

Background: Presentation with massive systemic embolization as the initial manifestation of occult malignancy is infrequent. The standard management of cancer-related arterial thromboembolism has not yet been established.

Case Presentation: We described a case of Trousseau's syndrome resulting in acute ischemic stroke concomitant with multiple embolizations in the spleen and kidney during oral administration of dabigatran for pulmonary embolism preceding the diagnosis of a malignant tumor. A cancer-related hypercoagulable state was suspected because the patient was admitted to the neurology department due to acute ischemic stroke with three territory infarcts on diffusion-weighted imaging (DWI) in the absence of identifiable conventional risk factors and brain vessel narrowing. The patient was subsequently diagnosed with epidermal growth factor receptor (EGFR) mutation-positive non-small-cell lung cancer (NSCLC) (stage IV) with pleural metastasis. Administration of low-molecular-weight heparin followed by long-term dabigatran under effective cancer therapy comprising gefitinib and subsequent chemotherapy did not cause stroke relapse during the 1-year follow-up.

Conclusions: This case suggests that cancer-related hypercoagulability should be considered an important etiology for stroke patients who develop unexplained disseminated acute cerebral infarction without conventional stroke risk factors, especially concomitant with multiple organ embolization. Novel oral anticoagulants may be an alternative therapy for the long-term management of cancer-related arterial thromboembolism under effective cancer therapy.
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http://dx.doi.org/10.1186/s12883-021-02236-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8188717PMC
June 2021

Spatial micro-variation of 3D hydrogel stiffness regulates the biomechanical properties of hMSCs.

Biofabrication 2021 Jun 9. Epub 2021 Jun 9.

Zhejiang Hospital, No. 12 Lingyin Road, Hangzhou, Zhejiang, 310013, CHINA.

Human mesenchymal stem cells (hMSCs) are one of the most promising candidates for cell-based therapeutic products. Nonetheless, their biomechanical phenotype after in vitro expansion is still unsatisfactory, for example, restricting the efficiency of microcirculation of delivered hMSCs for further cell therapies. Here, we propose a scheme using maleimide-dextran hydrogel with locally varied stiffness in microscale to modify the biomechanical properties of hMSCs in three-dimensional (3D) niches. We show that spatial micro-variation of stiffness can be controllably generated in the hydrogel with heterogeneously cross-linking via atomic force microscopy (AFM) measurements. The result of 3D cell culture experiment demonstrates the hydrogels trigger the formation of multicellular spheroids, and the derived hMSCs could be rationally softened via adjustment of the stiffness variation (SV) degree. Importantly, in vitro, the hMSCs modified with the higher SV degree can pass easier through capillary-shaped micro-channels. Further, we discuss the underlying mechanics of the increased cellular elasticity by focusing on the effect of rearranged actin networks, via the proposed microscopic model of biomechanically modified cells. Overall, this work highlights the effectiveness of SV-hydrogels in reprogramming and manufacturing hMSCs with designed biomechanical properties for improved therapeutic potential.
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http://dx.doi.org/10.1088/1758-5090/ac0982DOI Listing
June 2021

Web repositories of natural agents promote pests and pathogenic microbes management.

Brief Bioinform 2021 Jun 7. Epub 2021 Jun 7.

State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Center for Research and Development of Fine Chemicals, Guizhou University, Guiyang 550025, P. R. China.

The grand challenge to meet the increasing demands for food by a rapidly growing global population requires protecting crops from pests. Natural active substances play a significant role in the sustainable pests and pathogenic microbes management. In recent years, natural products- (NPs), antimicrobial peptides- (AMPs), medicinal plant- and plant essential oils (EOs)-related online resources have greatly facilitated the development of pests and pathogenic microbes control agents in an efficient and economical manner. However, a comprehensive comparison, analysis and summary of these existing web resources are still lacking. Here, we surveyed these databases of NPs, AMPs, medicinal plants and plant EOs with insecticidal, antibacterial, antiviral and antifungal activity, and we compared their functionality, data volume, data sources and applicability. We comprehensively discussed the limitation of these web resources. This study provides a toolbox for bench scientists working in the pesticide, botany, biomedical and pharmaceutical engineering fields. The aim of the review is to hope that these web resources will facilitate the discovery and development of potential active ingredients of pests and pathogenic microbes control agents.
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http://dx.doi.org/10.1093/bib/bbab205DOI Listing
June 2021

Identification of a CD4-binding site-directed antibody with ADCC activity from a chronic HIV-1B'-infected Chinese donor.

Virus Res 2021 Jun 15;302:198470. Epub 2021 Jun 15.

State Key Laboratory of Infectious Disease Prevention and Control, National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention, 155 Changbai Road, Changping District, Beijing 102206, China; Division of Research of Virology and Immunology, National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention, 155 Changbai Road, Changping District, Beijing 102206, China. Electronic address:

Antibody-dependent cell-mediated cytotoxicity (ADCC) plays an important role in controlling HIV-1 invasion and replication in vivo. Isolation and identification of monoclonal antibodies (mAbs) with ADCC activity help design effective vaccines and develop novel treatment strategies. In this study, we first identified a broad neutralizer who had been infected with an HIV-1B' strain for over 10 years. Next, through probe-specific single-B-cell sorting and PCR amplification, we obtained genes for variable regions of the heavy chain (VHs) and light chain (VLs) of six antibodies and ligated them into expression vectors. After antibody expression and ELISA screening, we obtained a CD4-binding site-directed antibody (451-B4), whose VH and VL originated from the IGHV1-24 and IGLV1-40 germlines, respectively. Although 451-B4 neutralized only the SF162 tier 1 pseudovirus and 398F1 tier 2 pseudovirus, it could mediate comparable ADCC activity to a broadly neutralizing antibody, VRC01. The 451-B4 antibody will be a useful candidate for developing an ADCC-based treatment strategy against HIV-1 replication or latent infection in vivo.
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http://dx.doi.org/10.1016/j.virusres.2021.198470DOI Listing
June 2021

Lon protease downregulates phenazine-1-carboxamide biosynthesis by degrading the quorum sensing signal synthase PhzI and exhibits negative feedback regulation of Lon itself in Pseudomonas chlororaphis HT66.

Mol Microbiol 2021 Jun 7. Epub 2021 Jun 7.

State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.

Pseudomonas chlororaphis HT66 exhibits strong antagonistic activity against various phytopathogenic fungi due to its main antibiotic phenazine-1-carboxamide (PCN). PCN gene cluster consists of phzABCDEFG, phzH, phzI, and phzR operons. phzABCDEFG transcription is activated by the PhzI/R quorum sensing system. Deletion of the lon gene encoding an ATP-dependent protease resulted in significant enhancement of PCN production in strain HT66. However, the regulatory pathway and mechanism of Lon on PCN biosynthesis remain unknown. Here, lon mutation was shown to significantly improve antimicrobial activity of strain HT66. The N-acyl-homoserine lactone synthase PhzI mediates the negative regulation of PCN biosynthesis and phzABCDEFG transcription by Lon. Western blot showed that PhzI protein abundance and stability were significantly enhanced by lon deletion. The in vitro degradation assay suggested that Lon could directly degrade PhzI protein. However, Lon with an amino acid replacement (S -A) could not degrade PhzI protein. Lon-recognized region was located within the first 50 amino acids of PhzI. In addition, Lon formed a new autoregulatory feedback circuit to modulate its own degradation by other potential proteases. In summary, we elucidated the Lon-regulated pathway mediated by PhzI during PCN biosynthesis and the molecular mechanism underlying the degradation of PhzI by Lon in P. chlororaphis HT66.
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http://dx.doi.org/10.1111/mmi.14764DOI Listing
June 2021

Chiral Bidentate Phosphoramidite-Pd Catalyzed Asymmetric Decarboxylative Dipolar Cycloaddition for Multistereogenic Tetrahydrofurans with Cyclic -Sulfonyl Ketimine Moieties.

Org Lett 2021 Jun 7;23(12):4715-4720. Epub 2021 Jun 7.

State Key Laboratory of Organometallic Chemistry, Center for Excellence in Molecular Synthesis, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China.

An asymmetric [3 + 2] cycloaddition of vinyl ethylenecarbonates (VECs) and ()-3-arylvinyl substituted benzo[] isothiazole 1,1-dioxides has been developed using the Pd complex of a bidentate phosphoramidite (Me-BIPAM) as the catalyst, providing a wide variety of chiral multistereogenic vinyltetrahydrofurans in good yields with excellent diastereo- and enantioselectivities (up to >20:1 , 99% ).
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http://dx.doi.org/10.1021/acs.orglett.1c01411DOI Listing
June 2021

Efficient photocatalytic hydrogen evolution on single-crystalline metal selenide particles with suitable cocatalysts.

Chem Sci 2020 Apr 1;11(25):6436-6441. Epub 2020 Apr 1.

Research Initiative for Supra-Materials, Interdisciplinary Cluster for Cutting Edge Research, Shinshu University 4-17-1 Wakasato, Nagano-shi Nagano 380-8553 Japan

It is important to improve the apparent quantum yields (AQYs) of narrow bandgap photocatalysts to achieve efficient H production. The present work demonstrates a particulate solid solution of zinc selenide and copper gallium selenide (denoted as ZnSe:CGSe) that evolves H efficiently and is responsive to visible light up to 725 nm. This material was synthesized using a flux-assisted method and was found to comprise single-crystalline tetrahedral particles. The coloading of Ni and Rh, Pt, Pd or Ru as cocatalysts further improved the photocatalytic H evolution rate over this photocatalyst. With the optimal coloading of a Ni-Ru composite cocatalyst, an AQY of 13.7% was obtained at 420 nm during a sacrificial H evolution reaction, representing the highest value yet reported for a photocatalyst with an absorption edge longer than 700 nm. The present study demonstrates that the preparation of single-crystalline particles and the rational assembly of composite cocatalysts are effective strategies that allow the efficient utilization of long wavelengths by metal selenide photocatalysts for solar fuel production.
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http://dx.doi.org/10.1039/d0sc01167cDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8159321PMC
April 2020

Efficient synthesis of cyclic amidine-based fluorophores 6π-electrocyclic ring closure.

Chem Sci 2020 Mar 13;11(14):3586-3591. Epub 2020 Mar 13.

Guangdong Key Laboratory of Chiral Molecular and Drug Discovery, School of Pharmaceutical Sciences, Sun Yat-Sen University Guangzhou 510006 China

Novel 10π-electron cyclic amidines with excellent fluorescence properties were synthesized by a general and efficient 6π-electrocyclic ring closure of ketenimine and imine starting from -sulfonyl triazoles and arylamines. The photophysical properties of cyclic amidine fluorophores have been studied in detail and have shown good properties of a large Stokes shift, pH insensitivity, low cytotoxicity and higher photostability, which have great potential for biological imaging. Furthermore, this novel fluorophore was successfully applied to the localization of the NK-1 receptor in living systems.
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http://dx.doi.org/10.1039/d0sc00798fDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8152618PMC
March 2020

Neonatal hypoxic-ischemic encephalopathy diagnosis and treatment: a National Survey in China.

BMC Pediatr 2021 06 5;21(1):261. Epub 2021 Jun 5.

Department of Neonatology, Children's Hospital of Fudan University, National Children's Medical Center, Shanghai, 200032, China.

Background: Neonatal hypoxic-ischemic encephalopathy (HIE) affects as many as 100,000 infants each year in China. Therapeutic hypothermia reduces HIE related mortality and long-term neurodevelopmental disabilities. National guidelines for HIE management were published a decade ago. This study aimed to investigate the current status of HIE diagnosis and treatment in China.

Method: This prospective cross-sectional national survey used a questionnaire evaluating practices related to HIE management. Descriptive statistics and Chi-square or Fisher's exact test were used, and a p-value of < 0.05 was considered significant.

Results: The 273 hospitals that completed the survey were located in 31 of the 34 provincial districts in China. Eighty-eight percent of the hospitals were Level III hospitals, and 74% treated 10 or more HIE cases annually. Awareness rates of the national guidelines for HIE diagnosis, HIE treatment, and therapeutic hypothermia protocol were 85, 63, and 78%, respectively. Neurological manifestations and blood gas were used as HIE diagnostic criteria by 96% (263/273) and 68% (186/273) of the hospitals, respectively. Therapeutic hypothermia was used in 54% (147/273) of hospitals. The percentage of general hospitals that implemented therapeutic hypothermia (43%, 71/165) was significantly lower than that in maternity and infant hospitals (67%, 49/73) (χ = 11.752, p = 0.001) and children's hospitals (77%, 27/35) (χ = 13.446, p < 0.001). Reasons for not providing therapeutic hypothermia included reduction of HIE cases in recent years (39%), high cost of cooling devices and treatment (31%), lack of training (26%), and safety concerns (4%). Among the hospitals that provided therapeutic hypothermia, 27% (39/147) were in full compliance with the recommended protocol. Eighty-one percent (222/273) of the hospitals treated HIE infants with putative neuroprotective agents alone or in combination with cooling. Ninety-one percent of the hospitals had long-term neurodevelopmental follow-up programs for infants with HIE.

Conclusions: There is significant heterogeneity in HIE diagnosis and treatment in China. Therapeutic hypothermia has not become a standard of care for neonatal HIE nationwide. Unproven agents are widely used for HIE treatment. Nationwide standardization of HIE management and dissemination of therapeutic hypothermia represent the opportunities to reduce mortality and improve long-term neurodevelopmental outcomes of children affected by HIE.
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http://dx.doi.org/10.1186/s12887-021-02737-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8178820PMC
June 2021

Coordination and Redox Dual-Responsive Mesoporous Organosilica Nanoparticles Amplify Immunogenic Cell Death for Cancer Chemoimmunotherapy.

Small 2021 Jun 3:e2100006. Epub 2021 Jun 3.

Institutes for Life Sciences, School of Medicine, South China University of Technology, Guangzhou International Campus, Guangzhou, Guangdong, 510006, China.

Amplifying the chemotherapy-driven immunogenic cell death (ICD) for efficient and safe cancer chemoimmunotherapy remains a challenge. Here, a potential ICD nanoamplifier containing diselenide-bridged mesoporous organosilica nanoparticles (MONs) and chemotherapeutic ruthenium compound (KP1339) to achieve cancer chemoimmunotherapy is tailored. KP1339-loaded MONs show controlled drug release profiles via glutathione (GSH)-responsive competitive coordination and matrix degradation. High concentration of MONs selectively evoked reactive oxygen species production, GSH depletion, and endoplasmic reticulum stress in cancer cells, thus amplifying the ICD of KP1339 and boosting robust antitumor immunological responses. After the combination of PD-L1 checkpoint blockade, cancer cell membrane-cloaked KP1339-loaded MONs not only regress primary tumor growth with low systemic toxicity, but also inhibit distant tumor growth and pulmonary metastasis of breast cancer. The results have shown the potential of coordination and redox dual-responsive MONs boosting amplified ICD for cancer chemoimmunotherapy.
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http://dx.doi.org/10.1002/smll.202100006DOI Listing
June 2021

Inferring Single-Cell 3D Chromosomal Structures Based on the Lennard-Jones Potential.

Int J Mol Sci 2021 May 31;22(11). Epub 2021 May 31.

Department of Computer Science, University of Miami, 1364 Memorial Drive, Coral Gables, FL 33124, USA.

Reconstructing three-dimensional (3D) chromosomal structures based on single-cell Hi-C data is a challenging scientific problem due to the extreme sparseness of the single-cell Hi-C data. In this research, we used the Lennard-Jones potential to reconstruct both 500 kb and high-resolution 50 kb chromosomal structures based on single-cell Hi-C data. A chromosome was represented by a string of 500 kb or 50 kb DNA beads and put into a 3D cubic lattice for simulations. A 2D Gaussian function was used to impute the sparse single-cell Hi-C contact matrices. We designed a novel loss function based on the Lennard-Jones potential, in which the ε value, i.e., the well depth, was used to indicate how stable the binding of every pair of beads is. For the bead pairs that have single-cell Hi-C contacts and their neighboring bead pairs, the loss function assigns them stronger binding stability. The Metropolis-Hastings algorithm was used to try different locations for the DNA beads, and simulated annealing was used to optimize the loss function. We proved the correctness and validness of the reconstructed 3D structures by evaluating the models according to multiple criteria and comparing the models with 3D-FISH data.
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http://dx.doi.org/10.3390/ijms22115914DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8199262PMC
May 2021

An Adaptive Unsupervised Feature Selection Algorithm Based on MDS for Tumor Gene Data Classification.

Sensors (Basel) 2021 May 23;21(11). Epub 2021 May 23.

School of Artificial Intelligence, Henan University, Kaifeng 475004, China.

Identifying the key genes related to tumors from gene expression data with a large number of features is important for the accurate classification of tumors and to make special treatment decisions. In recent years, unsupervised feature selection algorithms have attracted considerable attention in the field of gene selection as they can find the most discriminating subsets of genes, namely the potential information in biological data. Recent research also shows that maintaining the important structure of data is necessary for gene selection. However, most current feature selection methods merely capture the local structure of the original data while ignoring the importance of the global structure of the original data. We believe that the global structure and local structure of the original data are equally important, and so the selected genes should maintain the essential structure of the original data as far as possible. In this paper, we propose a new, adaptive, unsupervised feature selection scheme which not only reconstructs high-dimensional data into a low-dimensional space with the constraint of feature distance invariance but also employs ℓ2,1-norm to enable a matrix with the ability to perform gene selection embedding into the local manifold structure-learning framework. Moreover, an effective algorithm is developed to solve the optimization problem based on the proposed scheme. Comparative experiments with some classical schemes on real tumor datasets demonstrate the effectiveness of the proposed method.
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http://dx.doi.org/10.3390/s21113627DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8197094PMC
May 2021

Risk and prognostic nomograms for colorectal neuroendocrine neoplasm with liver metastasis: a population-based study.

Int J Colorectal Dis 2021 Jun 1. Epub 2021 Jun 1.

Research Center for Tissue Engineering and Regenerative Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.

Purpose: Liver metastasis (LM) significantly shortens the survival time of colorectal neuroendocrine neoplasms (NENs) patients. This research aimed to explore risk and prognostic factors in colorectal NENs patients with LM and develop nomograms for predicting the risk of LM and survival probability quantitatively.

Methods: A total of 9926 colorectal NENs patients registered in the Surveillance, Epidemiology, and End Results (SEER) database between 2010 and 2017 were included. Risk factors for LM in colorectal NENs patients were identified by multivariate logistic regression analysis. Potential prognostic factors for colorectal NENs patients with LM were identified by multivariable Cox regression analysis. Nomograms were constructed for quantifying the probability of LM occurrence and survival.

Results: At diagnosis, 8.7% of colorectal NENs patients suffered LM, with 1-, 3-, and 5-year cancer-specific survival (CSS) rates of 44.3%, 26.5%, and 18.0%, respectively. Factors associated with LM occurrence included gender, age at diagnosis, primary tumor location, carcinoembryonic antigen (CEA), histological differentiation, T stage, and N stage. Age at diagnosis, race, histological differentiation, N stage, tumor size, primary tumor location, primary site surgery, and extraliver metastasis were prognostic factors of cancer-specific mortality. The area under the receiver operating characteristics (ROC) curve of the nomogram for predicting LM was 0.888 (95% CI: 0.877-0.898), and the C-index of the nomogram for estimating CSS probability was 0.705 (95% CI: 0.682-0.729).

Conclusions: This research identified the risk and prognostic factors in colorectal NENs patients with LM. The nomograms constructed by this study can be convenient tools for facilitating clinical decision-making.
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http://dx.doi.org/10.1007/s00384-021-03920-yDOI Listing
June 2021