Publications by authors named "Zheng Wang"

3,263 Publications

  • Page 1 of 1

Facile Synthesis of Carbon Nanobelts Decorated with Cu and Pd for Nitrate Electroreduction to Ammonia.

ACS Appl Mater Interfaces 2022 Jun 28. Epub 2022 Jun 28.

Department of Applied Chemistry, School of Advanced Materials and Nanotechnology, Xidian University, Xi'an 710126, Shaanxi, China.

The electrocatalytic nitrate conversion of ammonia at ambient conditions provides not only a solution for restoring the imbalance in the global nitrogen cycle but also a sustainable alternative for the Haber-Bosch process. However, large-scale and efficient application of electrocatalytic denitrification has been limited by the lack of active catalysts with a high selectivity of nitrate reduction to N. In this work, we present a one-step solution processed synthetic strategy at low temperature to prepare carbon-nanobelts-supported uniform Cu and Pd nanoclusters. It is found that Cu catalyzed the formation of carbon nanobelts. The prepared samples were used for the green synthesis of ammonia from nitrate by electrocatalysis. For the nitrate reduction reaction (NORR), Cu-Pd/C nanobelts show higher activity than Cu/C nanobelts, achieving a high yield of ammonia of 220.8 μg mg h with a Faradaic efficiency (FE) of 62.3% at -0.4 V vs RHE (reversible hydrogen electrode), while for the nitrite reduction reaction (NORR), a high FE of 95% at -0.2 V vs RHE can be obtained for Cu/C nanobelts with the yield of ammonia increased with the negative shift of the applied potentials. Theoretical calculations demonstrated that Pd and Cu are responsible for hydrogen evolution reaction (HER) and NORR, respectively.
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http://dx.doi.org/10.1021/acsami.2c09357DOI Listing
June 2022

The presence and degradation of nerve fibers in articular cartilage of neonatal rats.

J Orthop Surg Res 2022 Jun 27;17(1):331. Epub 2022 Jun 27.

Department of Orthopaedics, Xijing Hospital, Air Force Medical University, Xi'an, 710032, Shanxi, China.

Purpose: To investigate the presence and change of nerve fibers and neuropeptide during early development of articular cartilage in neonatal rats.

Methods: Articular cartilage in distal-femoral epiphyses was collected from neonatal Sprague Dawley rats, which were 1-day, 5-day, and 10-day postnatal (P1, P5 and P10). Microscopy, immunofluorescence, transmission and scanning electron microscopy (TEM and SEM) were performed for detection of nerve fibers. Quantitative analysis for substance P (SP) and neuropeptide Y (NPY) was conducted using immunofluorescence and enzyme-linked immunosorbent assay (ELISA).

Results: TEM showed the existence of myelinated nerve fibers in the extracellular matrix of articular cartilage in both P1, P5 and P10 rats, and they formed synaptic contacts with chondrocytes. During this time, chondrocytes proceeded with their development, and the nerve fibers gradually degraded. The ELISA results showed significant increase of the sensory neuropeptide SP and the sympathetic neuropeptide NPY in the cartilage tissue. Immunofluorescence results showed the distribution of SP and NPY in the perichondrium, the cartilage canals, the plasma of chondrocytes, and extracellular matrix in the cartilage tissue.

Conclusions: Nerve fibers exist in the matrix of articular cartilage during early development of knee joints in neonatal rats. Nerve fibers form synaptic contacts with chondrocytes at the early stage and then degrade gradually in the course of chondrocyte development. SP and NPY significantly increase in articular cartilage during this very period. These results indicate that the nerve fibers and the neuropeptide they secrete may exert important effect on the development of articular cartilage.
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http://dx.doi.org/10.1186/s13018-022-03221-2DOI Listing
June 2022

Reduced number of IFN-γ producing cells in peripheral blood is a biomarker for patients with renal cell carcinoma.

Immun Inflamm Dis 2022 Jul;10(7):e637

Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

Renal cell cancer (RCC) is the most lethal of all the common urologic cancers and constitutes 2.2% of all malignancy diagnoses. The incidence of RCC has been steadily increasing in recent decades. The classic risk factors of RCC include smoking, hypertension, obesity, genetics, and genetic mutations. Recent studies also revealed that RCC was an immunogenic tumor and affected by host immune status. Among the pan-cance, RCC presented with the highest degree of immune infiltration, indicating RCC patients might benefit from immunotherapy. A new immune classification of RCC has been developed by Su et al. based on tumor-infiltrating lymphocytes to guide clinical practice. However, these studies mainly focus on biomarkers derived from tumor microenvironment (TME), the biomarkers based on peripheral blood samples to RCC have rarely been described. We collected peripheral blood samples from RCC patients and their matched healthy controls and detected the number of IL-2 and IFN-γ producing cells by implementing an enzyme-linked immunospot (ELISPOT) assay. This is the first study to report blood-based immune biomarkers for RCC using an ELISPOT assay. Our results suggested the frequency of IFN-γ producing cells but not IL-2 producing cells was associated with RCC risk. These findings warrant further validation in larger prospective studies.
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http://dx.doi.org/10.1002/iid3.637DOI Listing
July 2022

The key role of hydrophobicity in the determination of pharmaceuticals by liquid chromatography-electrospray ionization-mass spectrometry under the interference of natural organic matter.

Environ Sci Pollut Res Int 2022 Jun 27. Epub 2022 Jun 27.

State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210023, China.

The determination of trace-level pharmaceuticals in water is generally performed using liquid chromatography combined with mass spectrometry, which is susceptible to interference from non-target substances, such as natural organic matter (NOM). In this study, the interference of NOM on the determination of 20 typical pharmaceuticals using solid-phase extraction followed by ultra-performance liquid chromatography-electrospray ionization-triple quadrupole mass spectrometry (UPLC-ESI-tqMS) was investigated with a combined consideration of recoveries, matrix effects, and process efficiencies. The results showed that the recoveries of most pharmaceuticals were not significantly affected by NOM concentrations of 1-50 mg/L. The matrix effects and process efficiencies decreased linearly with increasing logarithmic NOM concentrations, and the changes in matrix effects and process efficiencies both exhibited negative linear correlations with the pharmaceuticals' hydrophobicity (logK). This result indicated that the determination of hydrophilic pharmaceuticals suffered from more severe NOM interference, as NOM entered the ESI source together with hydrophilic pharmaceuticals after UPLC separation and subsequently weakened the ionization efficiency of these pharmaceuticals. According to the correlations between logK and the changes in matrix effects and process efficiencies, the pharmaceutical determination in positive/negative ESI modes with logK ≤ 3.80/4.27 is considered to be significantly affected by NOM, accompanied by > 20% changes in matrix effects and process efficiencies.
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http://dx.doi.org/10.1007/s11356-022-21674-0DOI Listing
June 2022

The Impact of Authentic Leadership on Innovative Work Behavior: Mediating Roles of Proactive Personality and Employee Engagement.

Front Psychol 2022 9;13:879176. Epub 2022 Jun 9.

School of Continuing Education, China University of Political Science and Law, Beijing, China.

The purpose of the study is to investigate the impact of authentic leadership and proactive personality on innovative work behavior through dual mediation approach. This study applied a judgment sampling technique and data were gathered from 311 high-tech manufacturing industries of Shenzhen, China. The measurement model and structural model were tested using structural equation modeling technique through AMOS 24 software. The results indicate that authentic leadership has a positive and significant effect on proactive personality. Meanwhile, findings show that proactive personality has a significant impact on innovative work behavior. Moreover, findings show that proactive personality positively mediates the relationship between authentic leadership and innovative work behavior. Furthermore, findings illustrate that work engagement positively mediates the relationship between proactive personality and innovative work behavior. This study provides insightful and valuable implications to high-tech manufacturing industries executives and regulators interested in organizational productivity.
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http://dx.doi.org/10.3389/fpsyg.2022.879176DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9226888PMC
June 2022

Lycorine and organ protection: Review of its potential effects and molecular mechanisms.

Phytomedicine 2022 Jun 9;104:154266. Epub 2022 Jun 9.

Department of Cardiology, Xi'an No.3 Hospital, Faculty of Life Sciences and Medicine, Northwest University, Xi'an, China; Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education. Faculty of Life Sciences, Northwest University, Xi'an, China. Electronic address:

Background: Multiorgan dysfunction, especially sepsis-related multiorgan damage, remains a major cause of high mortality in the late stages of infection and a great clinical challenge. In recent years, natural drugs have received widespread attention because of their low cost, wide sources, high efficacy, low toxicity, and limited side effects. Lycorine, a natural compound extracted from Amaryllidaceae, exhibits multiple pharmacological activities, including in the regulation of autophagy and the induction of cancer cell apoptosis, and has anti-inflammatory, antifungal, antiviral, antimalarial, and antitumor activities. However, studies on lycorine have mainly focused on its antitumor properties, and research on its use for organ protection, especially in sepsis-related organ injury, is relatively limited.

Purpose: To review and discuss the effects and mechanisms of lycorine in the treatment of multi-organ dysfunction, especially sepsis.

Methods: Literature searches in electronic databases, such as Web of Science, Science Direct, PubMed, Google Scholar, and Scopus, were performed using 'Lycorine', 'Amaryllidaceae', 'Pharmacology', 'Pharmacokinetics', 'Anti-inflammation', 'Autophagy', 'Apoptosis', 'Anti-microbial and anti-parasitic', 'Antitumor', 'Organ protection', and 'Sepsis' as keywords, the correlated literature was extracted and conducted from the databases mentioned above.

Results: By summarizing the progress made in existing research, we found that the general effects of lycorine involve the regulation of autophagy and the induction of cancer cell apoptosis, and anti-inflammatory, antifungal, antiviral, antimalarial, and antitumor effects; through these pathways, the compound can ameliorate organ damage. In addition, lycorine was found to have an important effect on organ damage in sepsis.

Conclusion: Lycorine is a promising natural organ protective agent. This review will provide a new theoretical basis for the treatment of organ protection, especially in sepsis.
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http://dx.doi.org/10.1016/j.phymed.2022.154266DOI Listing
June 2022

Exenatide enhanced the antitumor efficacy on PD-1 blockade by the attenuation of neutrophil extracellular traps.

Biochem Biophys Res Commun 2022 Jun 18;619:97-103. Epub 2022 Jun 18.

Department of Respiratory and Critical Care Medicine, Beijing Institute of Respiratory Medicine and Beijing Chao-Yang Hospital, Capital Medical University, Beijing, 100020, China. Electronic address:

Neutrophil extracellular traps (NETs) are extracellular webs of DNA, histones, and granular contents, such as myeloperoxidase (MPO) and elastase, which are released by neutrophils. Reactive oxygen species (ROS) are involved in NETs formation that promote tumor progress. Exenatide could downregulate ROS production in some cell types. However, it is unknown whether Exenatide could influence tumor progress through NETs. Here, we constructed the LLC-based lung cancer and MC38-based colon cancer models and found that Exenatide treatment decreased tumor infiltrated NETs and peripheral MPO-DNA complex and elastase. In addition, the in vitro study showed that Exenatide decreased NETs formation and release. Furthermore, flow cytometry analysis showed that Exenatide treatment reduced ROS production in tumor infiltrated and in vitro neutrophils. However, the ROS inhibitor DPI counteracted the decease of tumor infiltrated and in vitro NETs formation and release by Exenatide. Functionally, the Exenatide/αPD-1 combination therapy was superior to single therapy in restricting tumor growth. Removement of NETs by DNase I weaken the enhancement of αPD-1 treatment by Exenatide. The enriched tumor infiltrated, spleen and lymph node CD8 T cells from combination therapy group secreted higher concentration of IFN-γ than single treatment. In addition, Exenatide exhibited no direct influence on IFN-γ secretion while purified NETs decreased IFN-γ secretion by CD8 T cells. The rechallenge study showed that the combination therapy activated long-term tumor rejection. In summary, our findings suggested that Exenatide might be a promising therapeutic candidate for enhancing PD-1 blockade in tumor treatment.
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http://dx.doi.org/10.1016/j.bbrc.2022.06.052DOI Listing
June 2022

scHiCEmbed: Bin-Specific Embeddings of Single-Cell Hi-C Data Using Graph Auto-Encoders.

Authors:
Tong Liu Zheng Wang

Genes (Basel) 2022 Jun 11;13(6). Epub 2022 Jun 11.

Department of Computer Science, University of Miami, 1365 Memorial Drive, P.O. Box 248154, Coral Gables, FL 33124, USA.

Most publicly accessible single-cell Hi-C data are sparse and cannot reach a higher resolution. Therefore, learning latent representations (bin-specific embeddings) of sparse single-cell Hi-C matrices would provide us with a novel way of mining valuable information hidden in the limited number of single-cell Hi-C contacts. We present scHiCEmbed, an unsupervised computational method for learning bin-specific embeddings of single-cell Hi-C data, and the computational system is applied to the tasks of 3D structure reconstruction of whole genomes and detection of topologically associating domains (TAD). The only input of scHiCEmbed is a raw or scHiCluster-imputed single-cell Hi-C matrix. The main process of scHiCEmbed is to embed each node/bin in a higher dimensional space using graph auto-encoders. The learned n-by-3 bin-specific embedding/latent matrix is considered the final reconstructed 3D genome structure. For TAD detection, we use constrained hierarchical clustering on the latent matrix to classify bins: is used to determine the optimal number of clusters, and each cluster is considered as one potential TAD. Our reconstructed 3D structures for individual chromatins at different cell stages reveal the expanding process of chromatins during the cell cycle. We observe that the TADs called from single-cell Hi-C data are not shared across individual cells and that the TAD boundaries called from raw or imputed single-cell Hi-C are significantly different from those called from bulk Hi-C, confirming the cell-to-cell variability in terms of TAD definitions. The source code for scHiCEmbed is publicly available, and the URL can be found in the conclusion section.
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http://dx.doi.org/10.3390/genes13061048DOI Listing
June 2022

Exploring the effects of substrate mineral fines on oil translocation in the shoreline environment: Experimental analysis, numerical simulation, and implications for spill response.

J Hazard Mater 2022 Jun 11;437:129341. Epub 2022 Jun 11.

Department of Building, Civil and Environmental Engineering, Concordia University, Montreal, QC H3G 1M8, Canada.

Mineral fines act a pivotal part in determining the fate and behavior of oil. In this study, the infiltrations of oil emulsion in simulated sediments and natural shoreline sediments were investigated using a fixed bed experiment. Oil infiltration process was simulated based on fixed-bed dispersion model. The role of mineral fines in oil release was explored using simulated and natural sediments. Although mineral fines exhibited a higher affinity for oil, it was found that increasing fines fractions decreased the flow rate of oil emulsion, thereby decreasing the oil retention in the sediment column. In terms of oil release from the sediment, the highest level of oil mass was observed in the oil-mineral flocculation phase compared to the water column and the water surface compartments. Compared to light crude oil, the release of engine oil from sediment was less. The effects of mineral fines on oil infiltration and release were also confirmed by using natural shoreline sediments. Results of our detailed field studies also showed that current shoreline classification datasets do not characterize the presence and fraction of mineral fines at a level of detail required to accurately predict the significance of oil translocation following spill incidents.
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http://dx.doi.org/10.1016/j.jhazmat.2022.129341DOI Listing
June 2022

Acute and Subacute Safety Evaluation of Black Tea Extract (Herbt Tea Essences) in Mice.

Toxics 2022 May 27;10(6). Epub 2022 May 27.

State Key Laboratory of Cellular Stress Biology, School of Life Sciences, The Fifth Hospital of Xiamen, Xiang'an Branch of the First Affiliated Hospital, Xiamen University, Xiamen 361102, China.

Theabrownin (TB) is a heterogeneous biomacromolecule, extracted from tea, with many functional groups. Importantly, TB possesses diverse health benefits, such as antitumor activity and blood lipid-lowering effects. Presently, the content of TB in tea extract is relatively low. Here, we obtained a deep-processed black tea extract with a high content of TB (close to 80%), which was named Herbt Tea Essences (HTE). Currently, this study was designed to evaluate the biosafety of high-content TB products on mice. We implemented acute and subacute toxic experiments to assess its safety on organs, the serum biochemical and molecular levels. In the acute exposure study, we found that the median lethal dose (LD) value of HTE was 21.68 g/kg (21.06-24.70 g/kg, greater than 5 g/kg), suggesting that HTE had a low acute toxicity. In the 28-day subacute exposure study, our results showed that no abnormal effects were observed in the 40 and 400 mg/kg/day HTE-treated groups. However, we observed slight nephrotoxicity in the 4000 mg/kg/day HTE-treated group. The HTE-induced nephrotoxic effect might involve the inflammatory response activation mediated by the nuclear transcription factor kappa-B (NF-κB) signaling pathway. This study would provide valuable data for the TB safety assessment and promote this natural biomacromolecule application in daily drinking.
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http://dx.doi.org/10.3390/toxics10060286DOI Listing
May 2022

Silk Fibroin/Hydroxyapatite Coating Improved Osseointegration of Porous Titanium Implants under Diabetic Conditions via Activation of the PI3K/Akt Signaling Pathway.

ACS Biomater Sci Eng 2022 Jun 20. Epub 2022 Jun 20.

Department of Orthopedics of General Hospital of Northern Theater Command, Shenyang 110016, Liaoning Province, China.

The application of three-dimensional printed porous titanium implants (TIs) is compromised in patients suffering from diabetes mellitus (DM), which disturbs the normal process of implant osseointegration, resulting in fixation failure. It was possibly because of reactive oxygen species (ROS) overproduction at the bone-implant interface. A silk fibroin-based hydroxyapatite (SF/HA) hybrid material emerged as a novel biological material for accelerating new bone formation. We proposed that the SF/HA hybrid coated titanium implant (SHT) could mitigate DM-mediated impaired osseointegration, which had never been reported previously. To test this assumption and further elucidate the mechanisms, primary rabbit osteoblasts were seeded on TIs or SHTs and cultured with normal serum, diabetic serum (DS), DS + N-acetyl-L-cysteine (NAC) (a potent ROS inhibitor), and DS + LY294002 (a specific PI3K/Akt inhibitor) for osteoblast behavior examinations. An animal study was performed on diabetic rabbits implanted with the two kinds of implants for osseointegration tests. DM-mediated ROS overproduction caused osteoblastic biological dysfunctions and apoptotic injury, associated with suppression of PI3K/Akt signaling in osteoblasts cultured on a TI substrate. Of note, the SHT substrate significantly suppressed ROS overproduction under diabetic conditions, improved osteoblast functional recovery including ameliorative osteoblast adhesion and morphology, improved cellular proliferation and differentiation, and abrogated apoptosis, which exhibited the same effect as NAC administration on the TI. The results were further corroborated by enhanced osteogenesis and osseointegration of SHTs in diabetic rabbits. Moreover, the aforesaid promotive effects afforded by the SF/HA coating were totally abolished with administration of LY294002 for blocking PI3K/Akt signaling. The above results collectively demonstrated that the SF/HA hybrid coating significantly ameliorated DM-mediated impaired osseointegration of the TI via reactivation of the ROS-mediated PI3K/Akt signaling pathway. The hybrid coating elicited a novel surface biofunctionalization strategy to attain favorable clinical performance of TI in diabetics.
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http://dx.doi.org/10.1021/acsbiomaterials.2c00023DOI Listing
June 2022

Infusion of Human Albumin on Acute Pancreatitis Therapy: New Tricks for Old Dog?

Front Pharmacol 2022 1;13:842108. Epub 2022 Jun 1.

Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

Human serum albumin (HSA) infusion is a common administration on acute pancreatitis therapy in the Intensive Care Unit (ICU), but its actual association with patients' outcomes has not been confirmed. The study is aimed to determine whether the in-hospital prognosis of ICU patients with acute pancreatitis could benefit from HSA. 950 acute pancreatitis patients diagnosed in 2008-2019 were extracted from the MIMIC-IV database as our primary study cohort. The primary outcome was in-hospital mortality. We also performed an external validation with a cohort of 104 acute pancreatitis patients after PSM matching from the eICU database. In MIMIC-IV, 228 acute pancreatitis patients received HSA infusion (Alb group) during their hospitalization, while 722 patients did not (non-Alb group). Patients in the Alb group presented a poorer survival curve than the non-Alb group, while this difference disappeared after PSM or IPTW matching (log-rank test: PSM: = 0.660, IPTW: = 0.760). After including covariates, no association was found between HSA infusion and patients' in-hospital mortality before and after matching (original cohort: HR: 1.00, 95% CI: 0.66-1.52, = 0.998). HSA infusion also did not benefit patients' 28-days or ICU mortality, while it was significantly associated with a longer duration of hospital and ICU. In addition, the initial serum albumin levels, infections, the total amount, or the initial timing of infusion did not affect the conclusion. Similarly, in the eICU cohort, HSA infusion was still not a beneficial prognostic factor for patients' in-hospital prognosis ( = 0.087). Intravenous human serum albumin infusion could not benefit acute pancreatitis patients' in-hospital prognosis and was associated with prolonged hospital and ICU duration.
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http://dx.doi.org/10.3389/fphar.2022.842108DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9198420PMC
June 2022

Nested miRNA Secondary Structure Is a Unique Determinant of miR159 Efficacy in .

Front Plant Sci 2022 2;13:905264. Epub 2022 Jun 2.

State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Innovative Academy for Seed Design, Chinese Academy of Sciences, Beijing, China.

MicroRNAs (miRNAs) are 20- to 24-nucleotide small RNAs, and whenever a pri-miRNA precursor includes another miRNA precursor, and both of these precursors may generate independent non overlapping mature miRNAs, we called them nested miRNAs. However, the functional and regulatory roles of nested miRNA structures in plants are still unknown. In this study, the nested miR159a structure, which consists of two nested miRNAs, miR159a.1, and miR159a.2, was used as a model to determine miRNA-mediated gene silencing in plants. Complementation analysis of nested miR159a structures revealed that the miR159a structure can differentially complement the phenotype, and a duplex nested structure in the tail end region of the pre-miR159a fold back may have a possible dominant function, indicating the importance of the flanking sequence of the stem in the cleavage of the mature miRNA. Furthermore, continuously higher expression of the miR159a.2 duplex in the severe leaf curl phenotype indicates that miR159a.2 is functional in and suggests that in plants, a miRNA precursor may encode multiple regulatory small RNAs. Taken together, our study demonstrates that the nested miR159a structure regulated by duplex mutations of miR159a has a unique pattern and provides novel insight into silencing efficacy of miR159a.
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http://dx.doi.org/10.3389/fpls.2022.905264DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201385PMC
June 2022

Nerve Injury and Regeneration after Neurolysis: Ethanol Combined with Iodine-125 Radioactive Seed versus Ethanol Alone in Rabbits.

J Vasc Interv Radiol 2022 Jun 16. Epub 2022 Jun 16.

Department of Minimally Invasive Tumor Therapies Center, Beijing Hospital, National Center of Gerontology; Institute of Geriatric Medicine, Chinese Academy of Medicine Sciences, Beijing, P.R. China; Graduate School of Peking Union Medical College; Chinese Academy of Medical Sciences, Beijing, P.R. China. Electronic address:

Purpose: This study aimed to prove the hypothesis that neurolysis based on ethanol injection in combination with iodine-125 (I) radioactive seed implantation could prolong nerve regeneration time compared with ethanol injection alone. Moreover, we assessed the grade of nerve injury using both methods.

Materials And Methods: Twenty female rabbits (mean 2.8 ± 0.2 kg) were randomly assigned to group A (neurolysis of the left brachial plexus nerve based on ethanol injection in combination with I radioactive seed implantation, n=10) and group B (neurolysis using ethanol injection alone, n=10). The right brachial plexus nerve was used as a blank control. Injury and regeneration of the brachial plexus nerve were analyzed using electromyography (EMG). Statistical tests were performed using the Mann-Whitney U test and repeated-measures analysis of variance. The results were verified with histopathological examinations.

Results: The overall postprocedural amplitude was significantly lower in group A than in group B (P=0.01), particularly in the second month after the procedure (P=0.036). However, no statistical difference in latency was observed between the two groups (P=0.103). Histopathological examination of both groups revealed Sunderland's third-degree peripheral nerve injury (PNI), mainly characterized by axon disintegration. The degree of nerve regeneration was significantly lower in group A than in group B.

Conclusion: Neurolysis based on ethanol injection in combination with I radioactive seed implantation can prolong the time of nerve regeneration compared to ethanol injection alone, although both methods resulted in Sunderland's third-degree PNI.
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http://dx.doi.org/10.1016/j.jvir.2022.06.006DOI Listing
June 2022

Chromosome-level and haplotype-resolved genome provides insight into the tetraploid hybrid origin of patchouli.

Nat Commun 2022 Jun 18;13(1):3511. Epub 2022 Jun 18.

Institute of Medicinal Plant Physiology and Ecology, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, China.

Patchouli (Pogostemon cablin (Blanco) Benth.), a member of the Lamiaceae family, is an important aromatic plant that has been widely used in medicine and perfumery. Here, we report a 1.94 Gb chromosome-scale assembly of the patchouli genome (contig N50 = 7.97 Mb). The gene annotation reveals that tandem duplication of sesquiterpene biosynthetic genes may be a major contributor to the biosynthesis of patchouli bioactivity components. We further phase the genome into two distinct subgenomes (A and B), and identify a chromosome substitution event that have occurred between them. Further investigations show that a burst of universal LTR-RTs in the A subgenome lead to the divergence between two subgenomes. However, no significant subgenome dominance is detected. Finally, we track the evolutionary scenario of patchouli including whole genome tetraploidization, subgenome divergency, hybridization, and chromosome substitution, which are the key forces to determine the complexity of patchouli genome. Our work sheds light on the evolutionary history of patchouli and offers unprecedented genomic resources for fundamental patchouli research and elite germplasm development.
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http://dx.doi.org/10.1038/s41467-022-31121-wDOI Listing
June 2022

Vitamin D Receptor-Dependent Protective Effect of Moderate Hypoxia in a Mouse Colitis Model.

Front Physiol 2022 27;13:876890. Epub 2022 May 27.

Department of Gastroenterology, PUMC Hospital, CAMS and PUMC, Beijing, China.

Although hypoxia is important for maintaining the intestinal barrier, its effect on the barrier during acute colitis and the underlying mechanisms are not fully understood. To explore the influence of hypoxia in dextran sulfate sodium (DSS)-induced colitis mice and the role of hypoxia-inducible factor (HIF) and vitamin D receptor (VDR) in the process. Colitis mice were subjected to hypoxia to detect intestinal barrier function changes. And the mechanisms were explored . First, compared with colitis mice without hypoxia stimulation, those with hypoxia stimulation showed significantly decreased pathological damage and improved permeability of the intestinal barrier. The expression of tight junction proteins (occludin, ZO-1), HIF-1α as well as VDR was up-regulated in colitis mice with hypoxia stimulation. However, in VDR gene knockout (KO)colitis mice, hypoxia treatment showed no protective effect, suggesting the VDR dependency of this effect. Similarly although hypoxia stimulation could enhance the single-layer epithelial transmembrane electrical resistance in DLD-1 and NCM460 cells, these effects disappeared in VDR-knockdown cells. Furthermore, over-expression of HIF-1α in DLD-1 and NCM460 increased the expression of VDR, whereas HIF-1α-knockdown reduced the VDR expression directly. Chromatin immunoprecipitation and luciferase assays confirmed that HIF-1α can bind to the promoter region of the VDR gene under hypoxia. Finally, compared with their wild-type siblings, VDR-KO mice showed reduced abundance of anaerobic bacteria and SCFA-producing bacteria. Hypoxia was protective against DSS-induced colitis, and VDR is instrumental in it. Furthermore, HIF-1α-VDR mediates the effect of hypoxia on the barrier function. Moreover, intestinal flora may be an important link between hypoxia and VDR.
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http://dx.doi.org/10.3389/fphys.2022.876890DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9195869PMC
May 2022

Circulating miR-141 as a potential biomarker for diagnosis, prognosis and therapeutic targets in gallbladder cancer.

Sci Rep 2022 Jun 16;12(1):10072. Epub 2022 Jun 16.

Department of Hepatobiliary Surgery, First Affiliated Hospital of Xi'an Jiaotong University, 277 West Yanta Road, Xi'an, 710061, Shaanxi Province, China.

MicroRNA-141(miR-141) has been reported to play vital roles in the regulation of carcinogenesis and cancer progression. However, the biological function of miR-141 in GBC has received less attention. The aim of this study was to estimate the potential value of the expression level of miR-141 as a diagnostic and prognostic blood-based biomarker in gallbladder cancer (GBC) patients. Meanwhile, to explore its biological role in GBC cells. RT-PCR was employed to confirm the expression of miR-141 in ten paired tissue samples (10 GBC tissues and 10 adjacent normal gallbladder tissues), GBC cell lines and peripheral blood specimens from 98 GBC patients and 60 healthy controls. MTT assay was used to evaluate the GBC cells proliferation and flow cytometry was used to detect the cell apoptosis. Receiver operating characteristic curve analysis and the area under the curve (AUC) were used to evaluate the value of miR-141 plasma levels for GBC diagnosis. Finally, clinicopathological and survival data of all GBC patients were collected and analyzed. Here, we confirmed that the expression of miR-141 were upregulated in primary gallbladder cancer cells and tissues compared with human gallbladder epithelial cells and adjacent normal tissues (P < 0.0001). Meanwhile, we found that downregulated expression of miR-141 by miR-141 inhibitor could induce apoptosis and inhibit proliferation of GBC cells. Additionally, elevated plasma miR-141 expression was also detected in the peripheral blood of GBC patients compared with healthy controls (P < 0.0001). The AUC value of miR-141 for GBC diagnosis was 0.894 (95% CI 0.843-0.945), which was more valuable than those including carcinoembryonic antigen (CEA) (0.713, 95% CI 0.633-0.793), carbohydrate antigen 125 (CA125) (0.837, 95% CI 0.776-0.899) and carbohydrate antigen 19-9 (CA19-9) (0.869, 95% CI 0.813-0.924). The high expression level of miR-141 in plasma was significantly associated with tumor invasion (P = 0.008), lymph node metastasis (P < 0.0001) and advanced pathologic tumor/node/metastasis (pTNM) stage (P = 0.009). More importantly, high plasma miR-141 expression was an independent prognostic factor for predicting poorer long-term survival in GBC patients. Elevated expression of circulating miR-141 in peripheral blood might be a potential novel biomarker for diagnosis and prognosis of GBC patients. Downregulated expression of miR-141 could inhibit proliferation and induce apoptosis of GBC cells, that provide a potential therapeutic target for GBC.
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http://dx.doi.org/10.1038/s41598-022-13430-8DOI Listing
June 2022

Revealing the nexus between tourism development and CO2 emissions in Asia: does asymmetry matter?

Environ Sci Pollut Res Int 2022 Jun 15. Epub 2022 Jun 15.

Department of Economics, Federal Urdu University, Islamabad, Pakistan.

Asia is one of the fastest-growing regions in international tourism, economic growth, and CO2 emissions around the globe. However, the relationships between tourism and CO emissions are little and unclearly identified. The purpose of the study is to explore the asymmetric nexus between tourism and CO emissions in a panel of five high emitters Asian countries covering the period of 1995-2019 by using panel-NARDL-AMG. The empirical results reveal that a positive shock in tourism arrivals increases CO emissions, while a negative shock also increases CO emissions in the long run. Moreover, a negative shock has a greater effect on CO emissions than a positive shock in Asia in the long run. Tourism receipts are expected to maintain the robustness of CO emissions in the long run in Asian economies. The authorities should develop and design green international tourism activities in the Asian economies.
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http://dx.doi.org/10.1007/s11356-022-21339-yDOI Listing
June 2022

LncRNA DSCAM-AS1 Negatively Interacts with miR-124 to Promote Hepatocellular Carcinoma Proliferation.

Crit Rev Eukaryot Gene Expr 2022 ;32(3):1-8

Geriatrics department, Hebei Youfu Hospital, Shijiazhuang City, Hebei Province, 050000, P.R. China.

It is known that the development of resistance of breast cancer cells to chemotherapy is at least partially mediated by the upregulation of long non-coding RNA DSCAM antisense RNA 1 (DSCAM-AS1). Therefore, DSCAM-AS1 may play a role in cancer biology. The differential expression of DSCAM-AS1 and miR-124 in hepatocellular carcinoma (HCC) was analyzed by quantitative polymerase chain reaction. One-way analysis of variance (ANOVA) Tukey test was used to analyze the differences in gene expression and cell proliferation in HCC cell lines. Expression levels of DSCAM-AS1 and miR-124 were compared between HCC and paired non-tumor tissues by paired t-test. The effects of transfections on SNU-449 and SNU-398 cell proliferation were analyzed by CCK-8 assay. DSCAM-AS1 was upregulated, while miR-124 was downregulated in HCC tissues. DSCAM-AS1 and miR-124 were inversely correlated across HCC tissues but not normal tissues. DSCAM-AS1 level increased, while miR-124 level decreased along with the clinical stage increasing. miR-124 overexpression increased DSCAM-AS1 level while DSCAM-AS1 overexpression decreased miR-124 level. In addition, miR-124 overexpression decreased HCC cell proliferation, while DSCAM-AS1 increased HCC cell proliferation. Moreover, DSCAM-AS1 overexpression reduced the effects of miR-124 overexpression. DSCAM-AS1 interacts with miR-124 to promote HCC proliferation.
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http://dx.doi.org/10.1615/CritRevEukaryotGeneExpr.2021039662DOI Listing
June 2022

Co-occurrence of sibling and peer bullying victimization and depression and anxiety among Chinese adolescents: The role of sexual orientation.

Child Abuse Negl 2022 Jun 10;131:105684. Epub 2022 Jun 10.

Department of Maternal and Child Health, Xiangya School of Public Health, Central South University, Changsha, China. Electronic address:

Background: Bullying victimization is a robust predictor of mental distress among lesbian/gay, bisexual, and questioning (LGBQ) adolescents. However, few research accounted bullying victimization within the context of both sibling and peer relationships.

Objective: To examine the effect of sibling and peer bullying victimization on depression and anxiety between heterosexual adolescents and LGBQ adolescents.

Method: This was a cross-sectional study with a random sample of 3062 Chinese senior high school students (56.0% female) aged 14 to 18. Related information was collected through a self-reported questionnaire. The associations were examined via a set of logistic regression analysis.

Results: Of the participants, 12.5% were victims of sibling bullying only, 10.1% reported peer victimization only, and 4.7% were involved in both sibling and peer victimization. Compared to heterosexuals, LGBQ adolescents had increased risks of sibling victimization only, peer victimization only, and both sibling and peer victimization. The prevalence of major depression and generalized anxiety disorder were 24.9% and 18.7% respectively. Bullying victimization and sexual orientation were independently associated with depression and anxiety. Furthermore, the stratified analysis indicated that sibling victimization only was significantly associated with depression and anxiety for heterosexual adolescents, while the associations were not significant for LGBQ adolescents.

Conclusions: Sexual orientation and bullying victimization are predictive factors of depression and anxiety. In particular, bullying victimization shows the different effects on depression and anxiety between heterosexual and LGBQ adolescents. Anti-bullying intervention could take distinct strategies based on different sexual orientation to reduce risks of adolescents' mental health problems.
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http://dx.doi.org/10.1016/j.chiabu.2022.105684DOI Listing
June 2022

Letter to the Editor: An ultra-sensitive assay using cell-free DNA fragmentomics for multi-cancer early detection.

Mol Cancer 2022 Jun 11;21(1):129. Epub 2022 Jun 11.

Department of Colorectal Surgery, Fudan University Shanghai Cancer Center, Shanghai, 200032, China.

Early detection can benefit cancer patients with more effective treatments and better prognosis, but existing early screening tests are limited, especially for multi-cancer detection. This study investigated the most prevalent and lethal cancer types, including primary liver cancer (PLC), colorectal adenocarcinoma (CRC), and lung adenocarcinoma (LUAD). Leveraging the emerging cell-free DNA (cfDNA) fragmentomics, we developed a robust machine learning model for multi-cancer early detection. 1,214 participants, including 381 PLC, 298 CRC, 292 LUAD patients, and 243 healthy volunteers, were enrolled. The majority of patients (N = 971) were at early stages (stage 0, N = 34; stage I, N = 799). The participants were randomly divided into a training cohort and a test cohort in a 1:1 ratio while maintaining the ratio for the major histology subtypes. An ensemble stacked machine learning approach was developed using multiple plasma cfDNA fragmentomic features. The model was trained solely in the training cohort and then evaluated in the test cohort. Our model showed an Area Under the Curve (AUC) of 0.983 for differentiating cancer patients from healthy individuals. At 95.0% specificity, the sensitivity of detecting all cancer reached 95.5%, while 100%, 94.6%, and 90.4% for PLC, CRC, and LUAD, individually. The cancer origin model demonstrated an overall 93.1% accuracy for predicting cancer origin in the test cohort (97.4%, 94.3%, and 85.6% for PLC, CRC, and LUAD, respectively). Our model sensitivity is consistently high for early-stage and small-size tumors. Furthermore, its detection and origin classification power remained superior when reducing sequencing depth to 1× (cancer detection: ≥ 91.5% sensitivity at 95.0% specificity; cancer origin: ≥ 91.6% accuracy). In conclusion, we have incorporated plasma cfDNA fragmentomics into the ensemble stacked model and established an ultrasensitive assay for multi-cancer early detection, shedding light on developing cancer early screening in clinical practice.
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http://dx.doi.org/10.1186/s12943-022-01594-wDOI Listing
June 2022

Impacts of terrestrial input on the distribution characteristics of microplastics in the East China Sea characterized by chromophoric dissolved organic matter (CDOM) analysis.

Sci Total Environ 2022 Sep 9;838(Pt 4):156599. Epub 2022 Jun 9.

School of Civil and Environmental Engineering, Ningbo University, Ningbo 315211, PR China. Electronic address:

Large quantities of microplastics are found in the East China Sea (ECS), however, the impacts of complicated terrestrial input on the distribution characteristics of microplastics have not been studied. Hence, we aimed to characterize the microplastic distribution in the ECS combined with the fluorescence characteristics of chromophoric dissolved organic matter (CDOM), a sensitive technique to trace terrestrial substances in seawater. The average microplastic abundance in the surface seawater of ECS was 34.73 ± 4.05 items/m and sites in the north ECS had a higher microplastic abundance (55.90 ± 2.47 items/m) than those in the southern region (11.22 ± 4.01 items/m), due to its proximity to the Yangtze River estuary and Hangzhou Bay. Polyethylene (PE, 44.2 %) was the most abundant microplastic type in the northern region, whereas polyethylene terephthalate (PET, 28.4 %) had a higher proportion in the south ECS. Besides, sites in the north ECS had a higher diversity index of microplastics, suggesting various sources of microplastic pollution. Interestingly, a stronger correlation with the diversity index was found for protein-like component C3 (R = 0.56) in northern regions compared to fulvic-like component C1 (R = 0.32) and humic-like component C2 (R = 0.28), suggesting the significant impact of anthropogenic discharge. Moreover, no correlation between fluorescence components and microplastic diversity index was found in the south ECS, indicating that CDOM can reflect the impact range of terrestrial input on the distribution characteristics of microplastics. This research might be useful in assessing and reducing the impact of terrestrial input on the distribution characteristics of microplastics in the ECS.
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http://dx.doi.org/10.1016/j.scitotenv.2022.156599DOI Listing
September 2022

Exploring the potential of UAV hyperspectral image for estimating soil salinity: Effects of optimal band combination algorithm and random forest.

Spectrochim Acta A Mol Biomol Spectrosc 2022 Oct 25;279:121416. Epub 2022 May 25.

College of Geography and Remote Sensing Sciences, Xinjiang University, Urumqi 830046, China; Xinjiang Key Laboratory of Oasis Ecology, Xinjiang University, Urumqi 830046, China; Key Laboratory of Smart City and Environment Modelling of Higher Education Institute, Urumqi 830046, China.

Hyperspectral remote sensing by unmanned aerial vehicle (UAV) is an important technical tool for rapid, accurate, and real-time monitoring of soil salinity in arid zone agroecosystems. However, the key to effective soil salinity (electrical conductivity, EC) prediction by UAV visible and near-infrared (Vis-NIR) spectroscopy depends on the selection of effective features selection techniques and robust prediction characteristics algorithms. Therefore, in this study, two advanced feature selection methods and two commonly used modeling methods were applied to predict and characterize the spatial patterns of soil salinity (EC). The aim of this study was to explore the predictive performance of different feature band selection methods and to identify a robust soil salinity mapping strategy. The results demonstrated that standard normal variate (SNV) pre-processing broadened the absorption characteristics of the spectrum. Compared with competitive adaptive reweighted sampling (CARS), the optimal band combination algorithm (OBCA) strengthened the correlation with soil salinity and had a higher variable importance in the modeling. Random forest (RF) was more stable in mapping the spatial pattern of surface soil salinity compared to the partial least squares regression model (PLSR). Our results confirm the effectiveness of OBCA and RF in the developing UAV remote sensing models for surface soil salinity estimation and mapping.
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http://dx.doi.org/10.1016/j.saa.2022.121416DOI Listing
October 2022

Biomechanical comparison of the femoral neck system versus InterTan nail and three cannulated screws for unstable Pauwels type III femoral neck fracture.

Biomed Eng Online 2022 Jun 10;21(1):34. Epub 2022 Jun 10.

Department of Orthopaedic, General Hospital of Ningxia Medical University, Yinchuan, 750004, Ningxia, China.

Background: There are a variety of internal fixation methods for unstable femoral neck fractures (FNFs), but the best method is still unclear. Femoral neck system (FNS) is a dynamic angular stabilization system with cross screws, and is a new internal fixation implant designed for minimally invasive fixation of FNFs. In this study, we conducted a biomechanical comparison of FNS, InterTan nail and three cannulated screws for the treatment of Pauwels III FNFs and investigate the biomechanical properties of FNS.

Methods: A total of 18 left artificial femurs were selected and randomly divide into Group A (fixation with FNS), Group B (fixation with InterTan nail) and Group C (fixation with three cannulated screws), with 6 specimens in each group. After creating Pauwels type III FNF models, the specimens in each were tested with non-destructive quasi-static tests, including torsion, A-P bending and axial compression tests. The average slope of the linear load-deformation curve obtained from quasi-static tests defines the initial torsional stiffness, A-P bending stiffness, and axial compression stiffness. After cyclic loading test was applied, the overall deformation of models and local deformation of implant holes in each group were assessed. The overall deformation was estimated as the displacement recorded by the software of the mechanical testing apparatus. Local deformation was defined as interfragmental displacement. Data were analyzed by one-way analysis of variance (ANOVA) followed by Bonferroni post hoc test using the SPSS software (version 24.0, IBM, New York, NY, USA). Correlation analysis was performed using Pearson's correlation analysis.

Results: Group B exhibited significantly higher axial stiffness and A-P bending stiffness than the other two groups (P < 0.01), while Group A had significantly higher axial stiffness and A-P bending stiffness than Group C (P < 0.01). Groups A and B exhibited significantly higher torsional stiffness than Group C (P < 0.01), no statistical significance was observed between Groups A and B (P > 0.05). Group B exhibited significantly lower overall and local deformations than the other two groups (P < 0.01), while Group A had significantly lower overall and local deformations than Group C (P < 0.01). Correlation analysis revealed positive correlation between axial stiffness and A-P bending stiffness (r = 0.925, P < 0.01), torsional stiffness (r = 0.727, P < 0.01), between torsional stiffness and A-P bending stiffness; negative correlation between overall, local deformations and axial stiffness (r = - 0.889, - 0.901, respectively, both P < 0.01), and positive correlation between the two deformations (r = - 0.978, P < 0.01).

Conclusion: For fixation of unstable FNFs, InterTan nail showed the highest axial stiffness and A-P bending stiffness, followed by FNS, and then three cannulated screws. Torsional stiffness of FNS was comparable to that of the InterTan nail. FNS, as a novel minimally invasive implant, can create good mechanical environment for the healing of unstable FNFs. Clinical studies are needed to confirm the potential advantages of FNS observed in this biomechanical study.
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http://dx.doi.org/10.1186/s12938-022-01006-6DOI Listing
June 2022

Ventilator-associated pneumonia prevention in the Intensive care unit using Postpyloric tube feeding in China (VIP study): study protocol for a randomized controlled trial.

Trials 2022 Jun 9;23(1):478. Epub 2022 Jun 9.

Department of Critical Care Medicine, Maoming People's Hospital, 101 Weimin Road, Maoming, 525000, Guangdong, China.

Background: Ventilator-associated pneumonia is a challenge in critical care and is associated with high mortality and morbidity. Although some consensuses on preventing ventilator-associated pneumonia are reached, it is still somewhat controversial. Meta-analysis has shown that postpyloric tube feeding may reduce the incidences of ventilator-associated pneumonia, which still desires high-quality evidence. This trial aims to evaluate the efficacy and safety profiles of postpyloric tube feeding versus gastric tube feeding.

Methods/design: In this multicenter, open-label, randomized controlled trial, we will recruit 924 subjects expected to receive mechanical ventilation for no less than 48 h. Subjects on mechanical ventilation will be randomized (1:1) to receive postpyloric or gastric tube feeding and routine preventive measures simultaneously. The primary outcome is the proportion of patients with at least one ventilator-associated pneumonia episode. Adverse events and serious adverse events will be observed closely.

Discussion: The VIP study is a large-sample-sized, multicenter, open-label, randomized, parallel-group, controlled trial of postpyloric tube feeding in China and is well-designed based on previous studies. The results of this trial may help to provide evidence-based recommendations for the prevention of ventilator-associated pneumonia.

Trial Registration: Chictr.org.cn ChiCTR2100051593 . Registered on 28 September 2021.
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http://dx.doi.org/10.1186/s13063-022-06407-5DOI Listing
June 2022

Gut Microbiota, Plasma Metabolomic Profiles, and Carotid Artery Atherosclerosis in HIV Infection.

Arterioscler Thromb Vasc Biol 2022 Jun 9:101161ATVBAHA121317276. Epub 2022 Jun 9.

Department of Epidemiology and Population Health, Albert Einstein College of Medicine, Bronx, New York. (Z.W., B.A.P., J.X., D.B.H., T.W., K.A., R.C.K., R.D.B., Q.Q.).

Background: Alterations in gut microbiota and blood metabolomic profiles have been implicated in HIV infection and cardiovascular disease. However, it remains unclear whether alterations in gut microbiota may contribute to disrupted host blood metabolomic profiles in relation to atherosclerosis, especially in the context of HIV infection.

Methods: We analyzed cross-sectional associations between gut microbiota features and carotid artery plaque in 361 women with or at high risk of HIV (67% HIV+), and further integrated plaque-associated microbial features with plasma lipidomic/metabolomic profiles. Furthermore, in 737 women and men, we examined prospective associations of baseline gut bacteria-associated lipidomic and metabolomic profiles with incident carotid artery plaque over 7-year follow-up.

Results: We found 2 potentially pathogenic bacteria, and , were associated with carotid artery plaque; while the beneficial butyrate producer was inversely associated with plaque. and were associated with multiple lipids/metabolites which were clustered into 8 modules in network. A module comprised of 9 lysophosphatidylcholines and lysophosphatidylethanolamines and a module comprised of 9 diglycerides were associated with increased risk of carotid artery plaque (risk ratio [95% CI], 1.34 [1.09-1.64] and 1.24 [1.02-1.51] per SD increment, respectively). Functional analyses identified bacterial enzymes in lipid metabolism associated with these plasma lipids. In particular, phospholipase A1 and A2 are the key enzymes in the reactions producing lysophosphatidylcholines and lysophosphatidylethanolamines.

Conclusions: Among individuals with or at high risk of HIV infection, we identified altered gut microbiota and related functional capacities in the lipid metabolism associated with disrupted plasma lipidomic profiles and carotid artery atherosclerosis.
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http://dx.doi.org/10.1161/ATVBAHA.121.317276DOI Listing
June 2022

Multi-element Simultaneous sensitization of solution cathode glow discharge atomic emission spectrometry by using portable semiconductor anode refrigeration.

Talanta 2022 Oct 1;248:123638. Epub 2022 Jun 1.

Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai, 201899, China; Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Sciences, Beijing, 100049, China. Electronic address:

In this study, a modified solution cathode glow discharge atomic emission spectrometry (SCGD-AES) was used to detect metal elements in electroplating sewage. The SCGD-AES device was equipped with a portable semiconductor anode refrigeration unit, which was built independently. The red-heat effect of tungsten electrode was alleviated by adding the portable refrigeration unit, thus improving thermal stability with the withstand voltage from 1040 V to 1140 V. Compared with the devices without semiconductor refrigeration, the chromium was excited more favorable when the discharge voltage increased, and the limit of detection (LOD) decreased by 8.5 times. Furthermore, the LODs of Zn, Cd, Ni, Cu and Pb decreased by 1.8-3.2 times, respectively, which realized the detection of elements in electroplating sewage and showed high performance in the field of trace elements analysis. Furthermore, the accuracy of the method was verified by stream sediment reference material (GBW07312), and the results were consistent with the certified values. The recoveries of elements added to industrial sewage and seawater range were from 90.5 to 98.7%, demonstrating good accuracy of the proposed method.
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http://dx.doi.org/10.1016/j.talanta.2022.123638DOI Listing
October 2022

Diversity and Species Composition of Bark and Ambrosia Beetles Captured Using Ethanol Baited Traps on Different Hosts in East Java, Indonesia.

Zool Stud 2021 8;60:e55. Epub 2021 Oct 8.

Laboratory of Invasion Biology, School of Agricultural Sciences, Jiangxi Agricultural University, Nangchang, Jiangxi 330045, China. E-mail: (Wang).

Bark and ambrosia beetles are a diverse group that causes widespread mortality of deciduous and coniferous trees. The present study aimed to investigate the species compositions and richnesses of bark and ambrosia beetles in six species of plant hosts in East Java, Indonesia. Bark and ambrosia beetles were sampled using bottle traps baited with ethanol. Studies were conducted at two sites of monoculture and polyculture systems for each host plant species. At each site, 20 ethanol-baited traps were deployed on a linear transect along the forest. Six host tree species examined were used, namely (Teak), (Clove), (Mahogany), (Sumatran Pine), (Moluccan Albizia), and (Mango). The data were analyzed using R software. A total of 4823 beetles were collected, representing 26 ambrosia beetle and eight bark beetle species. The abundance of bark and ambrosia beetles was significantly highest at the sites of (F = 13.88, < 0.01). showed a strong attraction to the ethanol lure and was the dominant beetle species (50.65% of the total number of individuals). The Shannon-Wiener diversity index of all beetles captured in this study was the highest in the polyculture (2.28) and the lowest in the polyculture (0.47). According to Bray-Curtis analysis, the monoculture and polyculture had a high similarity value of bark and ambrosia beetle species compositions (91% similar). There were no significant differences between two cultural systems of host plants in the compositions of bark and ambrosia beetle species (ANOSIM, R = -0.1537, = 0.961).
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http://dx.doi.org/10.6620/ZS.2021.60-55DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9121139PMC
October 2021

ESIPT-Active 8-Hydroxyquinoline-Based Fluorescence Sensor for Zn(II) Detection and Aggregation-Induced Emission of the Zn(II) Complex.

ACS Omega 2022 May 18;7(21):18017-18026. Epub 2022 May 18.

Key Laboratory of Chemical Additives for China National Light Industry, College of Chemistry and Chemical Engineering, Shaanxi University of Science and Technology, Xi'an 710021, People's Republic of China.

A D-π-A type quinoline derivative, 2-(((4-(1, 2, 2-triphenylvinyl)phenyl)imino)methyl)quinolin-8-ol (), was synthesized and structurally characterized. The five-membered ring formed by the O-H···N hydrogen bond in contributed to the excited-state intramolecular proton transfer (ESIPT) behavior of , which was further verified by theoretical computations. Upon coordination with Zn, the hydroxyl proton in was removed, resulting in the inhibition of ESIPT. In the meanwhile, the formed complex displayed aggregation-induced emission (AIE) character in THF/HO mixtures, which is conducive to the fluorescence enhancement in aqueous media. Structure analysis suggested that the origin of the AIE characteristic was attributed to restriction of intramolecular rotations along with the formation of -aggregates. Based on ESIPT coupled with AIE, could recognize Zn(II) in aqueous media via an orange fluorescence turn-on mode. Benefitting from the AIE property, chemosensor was successfully applied to fabricate test strips for rapid sensing of Zn(II) ions.
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http://dx.doi.org/10.1021/acsomega.2c01414DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9161411PMC
May 2022
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