Publications by authors named "Zheng Ren"

84 Publications

IGF2-AS knockdown inhibits glycolysis and accelerates apoptosis of gastric cancer cells through targeting miR-195/CREB1 axis.

Biomed Pharmacother 2020 Oct 24;130:110600. Epub 2020 Aug 24.

Department of Gastroenterology, Huaihe Hospital of Henan University, Kaifeng, Henan, China.

Dysregulation of long non-coding RNA (lncRNA) insulin growth factor 2 antisense (IGF2-AS) is being found to have relevance to tumorigenesis, including gastric cancer (GC). The purpose of this study was to further explore the detailed role and molecular mechanism of IGF2-AS in GC progression. The expression levels of IGF2-AS, miR-195 and cAMP responsive element binding protein 1 (CREB1) mRNA were assessed by qRT-PCR. Glucose consumption and lactate production were determined using a corresponding Commercial Assay Kit. Hexokinase 2 (HK2) and CREB1 protein levels were detected using western blot. Cell apoptosis was determined by flow cytometry. The targeted interaction between miR-195 and IGF2-AS or CREB1 was validated using dual-luciferase reporter and RNA immunoprecipitation (RIP) assays. Our data revealed that IGF2-AS was upregulated in GC tissues and predicted poor prognosis. IGF2-AS knockdown hampered glycolysis and accelerated apoptosis of GC cells. Moreover, IGF2-AS acted as a sponge of miR-195 and CREB1 was a direct target of miR-195. MiR-195 mediated the regulatory effect of IGF2-AS knockdown on GC cell glycolysis and apoptosis. MiR-195 exerted its regulatory effect on GC cell glycolysis and apoptosis by CREB1. Furthermore, IGF2-AS regulated CREB1 expression via sponging miR-195. In conclusion, our study suggested that IGF2-AS knockdown suppressed glycolysis and facilitated apoptosis in GC cells at least partly through sponging miR-195 and modulating CREB1 expression, highlighting a novel therapeutic strategy for GC treatment.
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http://dx.doi.org/10.1016/j.biopha.2020.110600DOI Listing
October 2020

Genomic Insights Into the Admixture History of Mongolic- and Tungusic-Speaking Populations From Southwestern East Asia.

Front Genet 2021 22;12:685285. Epub 2021 Jun 22.

Department of Forensic Medicine, Guizhou Medical University, Guiyang, China.

As a major part of the modern Eurasian or Altaic language family, most of the Mongolic and Tungusic languages were mainly spoken in northern China, Mongolia, and southern Siberia, but some were also found in southern China. Previous genetic surveys only focused on the dissection of genetic structure of northern Altaic-speaking populations; however, the ancestral origin and genomic diversification of Mongolic and Tungusic-speaking populations from southwestern East Asia remain poorly understood because of the paucity of high-density sampling and genome-wide data. Here, we generated genome-wide data at nearly 700,000 single-nucleotide polymorphisms (SNPs) in 26 Mongolians and 55 Manchus collected from Guizhou province in southwestern China. We applied principal component analysis (PCA), ADMIXTURE, statistics, analysis, , TreeMix, Fst, and ALDER to infer the fine-scale population genetic structure and admixture history. We found significant genetic differentiation between northern and southern Mongolic and Tungusic speakers, as one specific genetic cline of Manchu and Mongolian was identified in Guizhou province. Further results from ADMIXTURE and statistics showed that the studied Guizhou Mongolians and Manchus had a strong genetic affinity with southern East Asians, especially for inland southern East Asians. The -based estimates of ancestry admixture proportion demonstrated that Guizhou Mongolians and Manchus people could be modeled as the admixtures of one northern ancestry related to northern Tungusic/Mongolic speakers or Yellow River farmers and one southern ancestry associated with Austronesian, Tai-Kadai, and Austroasiatic speakers. The -based phylogeny and neighbor-joining tree further confirmed that Guizhou Manchus and Mongolians derived approximately half of the ancestry from their northern ancestors and the other half from southern Indigenous East Asians. The estimated admixture time ranged from 600 to 1,000 years ago, which further confirmed the admixture events were mediated the Mongolians Empire expansion during the formation of the Yuan dynasty.
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http://dx.doi.org/10.3389/fgene.2021.685285DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8258170PMC
June 2021

The prevalence of depressive symptoms and associated factors in middle-aged and elderly Chinese people.

J Affect Disord 2021 Jun 25;293:222-228. Epub 2021 Jun 25.

Department of Social Medicine and Health Management, School of Public Health, Jilin University, Changchun, China. Electronic address:

Background: This study aimed to evaluate the prevalence of depressive symptoms and associated factors in middle-aged and elderly people in China.

Methods: Data were obtained from the China Family Panel Studies (CFPS) 2018. We selected 16,767 respondents over the age of 45 as samples for research and analysis. A short version of the Center for Epidemiological Research Depression Scale (CES-D) was used to assess depressive symptoms. Chi-square test and multiple logistic regression analysis were used to explore the related factors of depressive symptoms. Back Propagation neural network model was established to examine the ranking of influencing factors of depressive symptoms.

Results: There was 24.1% prevalence of depressive symptoms among the respondents. The 14 important variables affecting depressive symptoms were screened out with the multivariable logistic regression. Based on the BP neural network to rank the degree of importance, the top five important variables were determined, which were subjective well-being (100.0%), self-reported health (92.0%), life satisfaction (83.4%), ADL disability (74.0%), and marital status (69.7%).

Limitations: This study was a cross-sectional study, and causality cannot be determined.

Conclusions: The prevalence of depressive symptoms in middle-aged and elderly Chinese people was high. Subjective quality of life evaluation, health condition and marital status were important factors that have an impact on depressive symptoms.
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http://dx.doi.org/10.1016/j.jad.2021.06.044DOI Listing
June 2021

Long noncoding RNA taurine-up regulated gene 1 for the prognosis of osteosarcoma: A protocol for meta-analysis and bioinformatics analysis.

Medicine (Baltimore) 2021 Jun;100(24):e26182

Department of Orthopedics and Soft Surgery, Gansu Cancer Hospital, Lanzhou, Gansu Province, China.

Background: In recent years, a variety of long noncoding RNA (lncRNA) has been confirmed to be involved in the initiation and progression of osteosarcoma. Taurine-up regulated gene 1 (TUG1) plays an important role in the formation, invasion, and metastasis of osteosarcoma. Therefore, perhaps TUG1 is a potential biomarker for the prognosis of patients suffering from osteosarcoma. In this study, meta-analysis and bioinformatics were adopted to further explore the effects of TUG1 on the prognosis of patients with osteosarcoma and its potential molecular mechanism.

Methods: Embase, PubMed, Sinomed, Web of Science, Cochrane Library, China National Knowledge Infrastructure, Wanfang database, and Vip Journal Integration Platform were searched from inception to May 2021. The relationship between TUG1 expression and survival outcome was estimated by hazard ratio (HRs) and 95% confidence interval (CIs). Meta-analysis was conducted on the Stata 16.0. The differential expression of TUG1 in osteosarcoma was analyzed by using UALCAN database, and the survival of TUG1 was analyzed as well. The target genes of TUG1 were predicted by RegRNA2.0 biology software, HMDD, targetscan and microTCDS, and TUG1-micoRNAs-mRNAs regulatory network was constructed. The predicted target genes obtained GeneOntology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) signal transduction pathway enrichment analysis using FunRich platform.

Results: The results of this meta-analysis would be submitted to peer-reviewed journals for publication.

Conclusion: This study will provide evidence-based medical evidence for the relationship between TUG1 and the prognosis of osteosarcoma. Furthermore, bioinformatics analysis will provide ideas for the exploration on osteosarcoma mechanism.

Ethics And Dissemination: The private information from individuals will not be published. This systematic review also should not damage participants' rights. Ethical approval is not available. The results will be published in a peer-reviewed journal or disseminated in relevant conferences.

Osf Registration Number: DOI 10.17605/OSF.IO/CW4BF.
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http://dx.doi.org/10.1097/MD.0000000000026182DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8213273PMC
June 2021

Use of an electronic seizure diary in a randomized, controlled trial of natalizumab in adult participants with drug-resistant focal epilepsy.

Epilepsy Behav 2021 05 5;118:107925. Epub 2021 Apr 5.

Biogen, Cambridge, MA, USA. Electronic address:

Objective: To analyze electronic diary (e-diary) use in a phase 2, randomized, controlled clinical trial (OPUS; NCT03283371) of natalizumab in adult participants with drug-resistant focal epilepsy.

Methods: We developed an e-diary, which incorporated an episodic seizure diary and a daily diary reminder, for use as the primary source to record participants' daily seizure activity in the OPUS phase 2 clinical trial. Participants and/or their designated caregivers made e-diary entries by selecting seizure descriptions generated in the participants' and/or caregivers' own words at the time of screening. Seizures and seizure-free days were reported for the current day and for up to 5 and 4 retrospective days, respectively. A record of seizure symptoms entered within the prior 5-day period was displayed on accessing the diary. Changes were not permitted in the e-diary once a seizure record was saved unless a data change request was made. A paper backup diary was available.

Results: E-diary entries (N = 15,176) from the 6-week baseline period and subsequent 24-week placebo-controlled period were analyzed for 66 adults who were randomized and dosed in the OPUS trial. The overall e-diary compliance, defined as the total number of days with any entry out of the total number of days in the baseline and placebo-controlled periods for all participants combined, was 83.6%. Caregivers made 190 (1.3%) e-diary entries. Day-of-event e-diary entries totaled 11,248 (74.1%). At least one paper backup diary was used by 36 (54.5%) participants.

Significance: Our data highlight that good e-diary compliance can be achieved across participants in randomized clinical trials in adult focal epilepsy. In addition to identifying and addressing any barriers that may prevent a minority of participants from achieving good e-diary compliance, consideration of e-diary elements, such as recall period and reporting of seizure-free days, will facilitate the most accurate data capture in epilepsy clinical trials.
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http://dx.doi.org/10.1016/j.yebeh.2021.107925DOI Listing
May 2021

Relationships of organisational justice, psychological capital and professional identity with job burnout among Chinese nurses: A cross-sectional study.

J Clin Nurs 2021 Apr 7. Epub 2021 Apr 7.

Department of Epidemiology and Biostatistics, School of Public Health, Jilin University, Changchun, China.

Aims And Objectives: The present study was aimed at describing the status of job burnout and exploring the mediating roles of psychological capital and professional identity on the association between organisational justice and job burnout.

Background: With the shortage of nurses all over the world, nurses' job burnout has become the focus of studies in recent years. However, limited published research has examined the mediating roles of psychological capital and professional identity on the association between organisational justice and job burnout within hospital contexts in China.

Design: A cross-sectional design.

Methods: A total of 1,009 nurses were enrolled from a major general hospital located in Qiqihar City in the northeast of China from March-July 2018, and asked to complete questionnaires regarding organisational justice, psychological capital, professional identity and job burnout. The STROBE checklist was adhered to in this study.

Results: It was observed that the overall proportion of nurses with job burnout was 58.8%. All the correlations among organisational justice, psychological capital, professional identity and job burnout were statistically significant, with coefficients ranging between -0.487-0.863. Psychological capital and professional identity had indirect effects equal to -0.072 and -0.142, respectively, on the association between organisational justice and job burnout, taking up 13.7% and 26.9% of the total effect, respectively. The serial indirect effect of psychological capital and professional identity was -0.129, accounting for 24.5% of the total effect.

Conclusions: This study showed the necessity of addressing the status of job burnout among Chinese nurses. Psychological capital and professional identity may mediate the linkage between organisational justice and job burnout independently and accumulatively.

Relevance To Clinical Practice: Nursing managers should strengthen organisational justice and improve the psychological capital and professional identity of nurses through effective strategies to prevent the job burnout of nurses.
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http://dx.doi.org/10.1111/jocn.15797DOI Listing
April 2021

A randomized study of natalizumab dosing regimens for relapsing-remitting multiple sclerosis.

Mult Scler 2021 Apr 6:13524585211003020. Epub 2021 Apr 6.

Biogen, Cambridge, MA, USA.

Background: REFINE was an exploratory, dose- and frequency-blinded, prospective, randomized, dose-ranging study in relapsing-remitting multiple sclerosis (RRMS) patients.

Objective: To examine the efficacy, safety, and tolerability of natalizumab administered via various regimens in RRMS patients.

Methods: Clinically stable RRMS patients previously treated with 300 mg natalizumab intravenously for ⩾12 months were randomized to one of six natalizumab regimens over 60 weeks: 300 mg administered intravenously or subcutaneously every 4 weeks (Q4W), 300 mg intravenously or subcutaneously every 12 weeks (Q12W), or 150 mg intravenously or subcutaneously Q12W. The primary endpoint was the mean cumulative number of combined unique active magnetic resonance imaging (MRI) lesions at week 60.

Results: In total, 290 patients were enrolled. All Q12W dosing arms were associated with increased clinical and MRI disease activity and closed early; ⩾39.5% of patients in each Q12W arm met rescue criteria. In the 300 mg intravenous and subcutaneous Q4 W arms, the mean cumulative number of combined unique active MRI lesions was 0.23 and 0.02, respectively; annualized relapse rates were 0.07 and 0.08, respectively; and trough natalizumab serum levels and α4-integrin saturation were comparable.

Conclusion: Natalizumab 300 mg subcutaneous Q4W was comparable to 300 mg intravenous Q4W dosing with respect to efficacy, pharmacokinetics/pharmacodynamics, and safety.
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http://dx.doi.org/10.1177/13524585211003020DOI Listing
April 2021

Establishment and associated factors of health records among young Chinese migrants.

Environ Health Prev Med 2021 Mar 24;26(1):39. Epub 2021 Mar 24.

Department of Epidemiology and Biostatistics, School of Public Health, Jilin University, Changchun, 130021, China.

Background: Most Chinese migrants have been faced with obstacles to getting access to local public health services. Young migrants among internal migrants make a major contribution to the economy. However, the establishment of their health records has been ignored. This research was aimed at exploring the status and associated factors of the establishment of health records among young Chinese migrants.

Methods: Data were obtained from the 2017 China Migrants Dynamic Survey (CMDS). Chi-square test and binary logistic regression were performed to investigate the associated factors of the establishment of health records among young Chinese migrants.

Results: Approximately 30.2% of young migrants had their health records established in inflow communities. Urban residence, medical insurance (insured), and lower average monthly household income were attributed to the establishment of more health records. Young migrants who were in northeast China and across provinces and immigrated for working or engaging in trade were less likely to have health records established. Young migrants who participated in social activities and public affairs activities and took type of people in touch as natives in the inflow area showed a higher possibility of establishing health records. Meanwhile, receiving health education and hearing about national basic public health services (BPHSs) were beneficial for establishing the health records of more young migrants.

Conclusion: This study showed that the health records of young migrants had a relatively low establishment rate. Sociodemographic and migration characteristics, health status, public health services, and social integration factors were all related to the health record establishment of young Chinese migrants.
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http://dx.doi.org/10.1186/s12199-021-00961-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7989406PMC
March 2021

Mimicking Neurotransmitter Activity and Realizing Algebraic Arithmetic on Flexible Protein-Gated Oxide Neuromorphic Transistors.

ACS Appl Mater Interfaces 2021 Feb 3;13(6):7784-7791. Epub 2021 Feb 3.

School of Physical Science and Technology, Ningbo University, Ningbo, Zhejiang 315211, People's Republic of China.

Recently, flexible neuromorphic devices have attracted extensive attention for the construction of perception cognitive systems with the ultimate objective to achieve robust computation, efficient learning, and adaptability to evolutionary changes. In particular, the design of flexible neuromorphic devices with data processing and arithmetic capabilities is highly desirable for wearable cognitive platforms. Here, an albumen-based protein-gated flexible indium tin oxide (ITO) ionotronic neuromorphic transistor was proposed. First, the transistor demonstrates excellent mechanical robustness against bending stress. Moreover, spike-duration-dependent synaptic plasticity and spike-amplitude-dependent synaptic plasticity behaviors are not affected by bending stress. With the unique protonic gating behaviors, neurotransmission processes in biological synapses are emulated, exhibiting three characteristics in neurotransmitter release, including quantal release, stochastic release, and excitatory or inhibitory release. In addition, three types of spike-timing-dependent plasticity learning rules are mimicked on the ITO ionotronic neuromorphic transistor. Most interestingly, algebraic arithmetic operations, including addition, subtraction, multiplication, and division, are implemented on the protein gated neuromorphic transistor for the first time. The present work would open a promising biorealistic avenue to the scientific community to control and design wearable "green" cognitive platforms, with potential applications including but not limited to intelligent humanoid robots and replacement neuroprosthetics.
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http://dx.doi.org/10.1021/acsami.0c22047DOI Listing
February 2021

Male-Dominated Migration and Massive Assimilation of Indigenous East Asians in the Formation of Muslim Hui People in Southwest China.

Front Genet 2020 11;11:618614. Epub 2021 Jan 11.

Department of Anthropology and Ethnology, Institute of Anthropology, School of Life Sciences, Xiamen University, Xiamen, China.

The origin and diversification of Muslim Hui people in China via demic or simple cultural diffusion is a long-going debate. We here generated genome-wide data at nearly 700,000 single nucleotide polymorphisms (SNPs) from 45 Hui and 14 Han Chinese individuals collected from Guizhou province in southwest China. We applied principal component analysis (PCA), ADMIXTURE, -statistics, , and analysis to infer the population genetic structure and admixture history. Our results revealed the Guizhou Hui people have a limited amount of West Eurasian related ancestry at a proportion of 6%, but show massive genetic assimilation with indigenous southern Han Chinese and Tibetan or Tungusic/Mongolic related northern East Asians. We also detected a high frequency of North Asia or Central Asia related paternal Y-chromosome but not maternal mtDNA lineages in Guizhou Hui. Our observation supports the cultural diffusion has played a vital role in the formation of Hui people and the migration of Hui people to southwest China was probably a sex-biased male-driven process.
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http://dx.doi.org/10.3389/fgene.2020.618614DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7834311PMC
January 2021

Relationships of professional identity and psychological reward satisfaction with subjective well-being among Chinese nurses.

J Nurs Manag 2021 Jan 27. Epub 2021 Jan 27.

Department of Epidemiology and Biostatistics, School of Public Health, Jilin University, Changchun, China.

Aim: This study aimed at exploring the impact of professional identity and psychological reward satisfaction on subjective well-being and clarifying the effect of psychological reward satisfaction on this relationship.

Background: People know little about the effect of psychological reward satisfaction on the relationship between professional identity and subjective well-being.

Methods: A cross-sectional survey was carried out on 1,009 nurses from Qiqihar City, Heilongjiang Province of China. Professional Identity Scale, Psychological Reward Satisfaction Scale and General Well-Being Schedule were used to assess professional identity, psychological reward satisfaction and subjective well-being, respectively. Associations were explored by using structural equation modelling.

Results: The subjective well-being of 436 (43.2%) nurses was at low and moderate levels. After the adjustment of potential confounding factors, professional identity was still associated with subjective well-being (B = 3.035, β = 0.215, p < .001). Professional identity (r = .308) and psychological reward satisfaction (r = .309) were positively correlated with subjective well-being. Psychological reward satisfaction mediated the association between professional identity and subjective well-being (effect = 0.114, p < .001).

Conclusion: This study suggested that the subjective well-being of nurses should be improved by paying special attention to them and taking targeted support measures.

Implications For Nursing Management: Nursing managers can help enhance the professional identity of nurses by organising nursing education activities, and pay more attention to psychological reward satisfaction to improve the subjective well-being of nurses.
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http://dx.doi.org/10.1111/jonm.13276DOI Listing
January 2021

Genetic structure and forensic characterization of 36 Y-chromosomal STR loci in Tibeto-Burman-speaking Yi population.

Mol Genet Genomic Med 2021 02 15;9(2):e1572. Epub 2021 Jan 15.

Department of Forensic Medicine, Guizhou Medical University, Guiyang, China.

Background: Male-specifically inherited Y-STRs have been widely used in population genetics and forensic investigations.

Methods: We genotyped and analyzed Y chromosome haplotypes of 408 unrelated Tibeto-Burman-speaking Yi male individuals from Guizhou using Goldeneye Y-PLUS kit. Population comparisons between the Guizhou Yi and 67 reference groups were performed via the AMOVA, MDS, and phylogenetic relationship reconstruction.

Results: A total of 389 alleles and 396 haplotypes could be detected, and the allelic frequencies ranged from 0.0025 to 0.9875. The haplotype diversity, random match probability, and discrimination capacity values were 0.9999, 0.0026, and 0.9900, respectively. The gene diversity (GD) of 36 Y-STR loci in the studied group ranged from 0.0248 (DYS645) to 0.9601 (DYS385a/b). Our newly genotyped Yi samples show a close affinity with other Tibeto-Burman speaking groups in China and Southeast Asia.

Conclusions: The population stratification was almost consistent with the geographic distribution and language-family, both among Chinese and worldwide ethnic groups. Our data may provide useful information for paternal lineage in the forensic application and population genetics, as well as evidence for archaeological and historical research.
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http://dx.doi.org/10.1002/mgg3.1572DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8077142PMC
February 2021

Nanoscale decoupling of electronic nematicity and structural anisotropy in FeSe thin films.

Nat Commun 2021 Jan 4;12(1):10. Epub 2021 Jan 4.

Department of Physics, Boston College, 140 Commonwealth Ave, Chestnut Hill, MA, 02467, USA.

In a material prone to a nematic instability, anisotropic strain in principle provides a preferred symmetry-breaking direction for the electronic nematic state to follow. This is consistent with experimental observations, where electronic nematicity and structural anisotropy typically appear hand-in-hand. In this work, we discover that electronic nematicity can be locally decoupled from the underlying structural anisotropy in strain-engineered iron-selenide (FeSe) thin films. We use heteroepitaxial molecular beam epitaxy to grow FeSe with a nanoscale network of modulations that give rise to spatially varying strain. We map local anisotropic strain by analyzing scanning tunneling microscopy topographs, and visualize electronic nematic domains from concomitant spectroscopic maps. While the domains form so that the energy of nemato-elastic coupling is minimized, we observe distinct regions where electronic nematic ordering fails to flip direction, even though the underlying structural anisotropy is locally reversed. The findings point towards a nanometer-scale stiffness of the nematic order parameter.
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http://dx.doi.org/10.1038/s41467-020-20150-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7782804PMC
January 2021

Association between lifestyle factors and depressive symptoms among Chinese middle school students: a cross-sectional study.

Psychol Health Med 2020 Sep 21:1-8. Epub 2020 Sep 21.

Department of Social Medicine and Health Management, School of Public Health, Jilin University, Changchun, Jilin, China.

This study aimed to identify the independent contributions of lifestyle factors to depressive symptoms among Chinese middle school students, with a focus on gender differences. A cross-sectional study of 3081 middle school students was conducted in Ganzhou City, Jiangxi Province, China. Students were asked to complete a questionnaire including socio-demographics, lifestyle factors, the Pittsburgh Sleep Quality Index and the Chinese Secondary School Students Depression Scale. The total prevalence of depressive symptoms was 19.9%. Poor quality of sleep, smoking, drinking and longer mobile phone use time were related to increased prevalence of depressive symptoms after adjusting for potential confounders. A significant interaction between gender and quality of sleep on the depressive symptoms was found ( = 0.014). The gender-stratified analysis showed that quality of sleep was significantly associated with depressive symptoms in both genders. However, the effect in males was greater than that in females.
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http://dx.doi.org/10.1080/13548506.2020.1823441DOI Listing
September 2020

Genetic structure and forensic characterisation of 36 Y-chromosomal STR loci in Hmong-Mien-speaking Miao population.

Ann Hum Biol 2020 Sep 9;47(6):541-548. Epub 2020 Jul 9.

Department of Forensic Medicine, Guizhou Medical University, Guiyang, Guizhou, China.

Background: Y-chromosomal short tandem repeats (Y-STRs) are widely used in paternity identification, pedigree investigation, and human population genetic history.

Aim: To investigate the Y-STR polymorphisms in a typical Miao population, and explore the genetic differentiation between the Miao population and reference groups.

Subjects And Methods: We detected 36 Y-STRs genotyping in 455 unrelated Miao individuals from Guizhou province, and analysed genetic differentiation between the Miao population and 76 reference groups.

Results: A total of 369 alleles were obtained, and the allele frequencies ranged from 0.0022 to 0.9802. In addition, the haplotype diversity, random match probability, and discrimination capacity values were 0.99997, 0.0022, and 0.9934, respectively. Moreover, the genetic relationships between Guizhou Miao and 76 ethnic populations showed that the population stratification was almost consistent with geographic distribution and language-family.

Conclusions: The 36 Y-STR loci in this study have good polymorphism distributions in the Guizhou Miao population, and therefore would be a useful tool in forensic identification and male parentage testing and even pedigree investigation.
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http://dx.doi.org/10.1080/03014460.2020.1788159DOI Listing
September 2020

Genome-wide analysis of unrecognised ethnic group Chuanqing people revealing a close affinity with Southern Han Chinese.

Ann Hum Biol 2020 Aug 3;47(5):465-471. Epub 2020 Jul 3.

Department of Anthropology and Ethnology, Institute of Anthropology, School of Sociology and Anthropology, and National Institute for Data Science in Health and Medicine, School of Life Sciences, Xiamen University, Xiamen, China.

Background: Chuanqing is an unrecognised ethnic group in Guizhou, southwest China. The genetic history of the Chuanqing people is hotly debated due to a lack of available genetic data.

Aim: To infer the genetic structure and population history of the Chuanqing people and genetic relationships of the Chuanqing with other East Asians.

Subjects And Methods: We collected samples from 14 Chuanqing individuals from Guizhou and genotyped about 690,000 genome-wide single nucleotide polymorphisms (SNPs). We used Principal Component Analysis (PCA), ADMIXTURE analysis, and statistics to infer the population genetic structure and admixture.

Results: Chuanqing people show a distinct genetic profile from indigenous Tai-Kadai and Tibeto-Burman speaking populations in southwest China, but they are genetically similar to southern Han Chinese, Miao, She and Tujia populations. The Han Chinese characteristic Y chromosomal lineages reach high frequencies in the Chuanqing.

Conclusions: The genetic formation of the Chuanqing people has been greatly influenced by Han Chinese related populations.
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http://dx.doi.org/10.1080/03014460.2020.1782470DOI Listing
August 2020

The relationship between job stress and job burnout: the mediating effects of perceived social support and job satisfaction.

Psychol Health Med 2021 02 10;26(2):204-211. Epub 2020 Jun 10.

Department of Social Medicine and Health Management, School of Public Health, Jilin University , Changchun, China.

Job stress, perceived social support, and job satisfaction are important predictors of job burnout. However, few studies have explored the mechanism of job stress on different dimensions of job burnout through perceived social support and job satisfaction among bank employees. In this study, a total of 1464 Chinese bank employees completed the Job Stress Assessment Scale, Perceived Social Support Scale, Minnesota Job Satisfaction Questionnaire and Maslach Burnout Inventory-General Survey. The results showed that job burnout was positively associated with job stress but negatively associated with perceived social support and job satisfaction. Mediating analyses showed that the mediation of perceived social support was different for job burnout than for different job burnout dimensions. Job satisfaction mediated the relationship between job stress and different job burnout dimensions. In addition, the association between job stress and job burnout was sequentially mediated by perceived social support and job satisfaction.
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http://dx.doi.org/10.1080/13548506.2020.1778750DOI Listing
February 2021

Artificial Tactile Perceptual Neuron with Nociceptive and Pressure Decoding Abilities.

ACS Appl Mater Interfaces 2020 Jun 29;12(23):26258-26266. Epub 2020 May 29.

School of Materials Science and Engineering, University of New South Wales (UNSW), Sydney, New South Wales 2052, Australia.

The neural system is a multifunctional perceptual learning system. Our brain can perceive different kinds of information to form senses, including touch, sight, hearing, and so on. Mimicking such perceptual learning systems is critical for neuromorphic platform applications. Here, an artificial tactile perceptual neuron is realized by utilizing electronic skins (E-skin) with oxide neuromorphic transistors, and this artificial tactile perceptual neuron successfully simulates biological tactile afferent nerves. First, the E-skin device is constructed using microstructured polydimethylsiloxane membranes coated with Ag/indium tin oxide (ITO) layers, exhibiting good sensitivities of ∼2.1 kPa and fast response time of tens of milliseconds. Then, the chitosan-based electrolyte-gated ITO neuromorphic transistor is fabricated and exhibits high performance and synaptic responses. Finally, the integrated artificial tactile perceptual neuron demonstrates pressure excitatory postsynaptic current and paired-pulse facilitation. The artificial tactile perceptual neuron is featured with low energy consumption as low as ∼0.7 nJ. Moreover, it can mimic acute and chronic pain and nociceptive characteristics of allodynia and hyperalgesia in biological nociceptors. Interestingly, the artificial tactile perceptual neuron can employ "Morse code" pressure-interpreting scheme. This simple and low-cost approach has excellent potential for applications including but not limited to intelligent humanoid robots and replacement neuroprosthetics.
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http://dx.doi.org/10.1021/acsami.0c04718DOI Listing
June 2020

Forensic Features and Population Genetic Structure of Dong, Yi, Han, and Chuanqing Human Populations in Southwest China Inferred From Insertion/Deletion Markers.

Front Genet 2020 30;11:360. Epub 2020 Apr 30.

Department of Forensic Medicine, Guizhou Medical University, Guiyang, China.

Guizhou province in southwest China has abundant genetic and cultural diversities, but the forensic features and genetic structure of Guizhou populations remain poorly understood due to the sparse sampling of present-day populations. Here, we present 30 insertion/deletion polymorphisms (InDels) data of 591 human individuals collected from four populations, Dong, Yi, Han, and Chuanqing residing in Guizhou. We calculated the forensic parameters of 30 InDel loci and found that this panel meets the efficiency of forensic personal identification based on the high combined power of discrimination, but it could only be used as a complementary tool in the parentage testing because of the lower combined probability of exclusion values. The studied populations are genetically closer related to geographically adjacent or linguistically related populations in southern China, such as the Tai-Kadai and Hmong-Mien speaking groups. The unrecognized ethnic Chuanqing people show an additional genetic affinity with Han Chinese, highlighting the role of possible military immigrations in their origin.
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http://dx.doi.org/10.3389/fgene.2020.00360DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7205039PMC
April 2020

Gender difference in the association of coping styles and social support with psychological distress among patients with end-stage renal disease.

PeerJ 2020 26;8:e8713. Epub 2020 Mar 26.

Department of Social Medicine and Health Management, School of Public Health, Jilin University, Changchun, China.

Objectives: The study aimed to explore the association of coping styles and social support with psychological distress among patients with end-stage renal disease (ESRD) as well as gender differences in these associations.

Methods: A cross-sectional study of 769 ESRD patients was conducted at 25 hospitals in Qiqihar City, China. All information was collected using structured questionnaires that were self-administered by the patients. Patients' psychological health status, coping styles and perceived social support were measured using the 12-item General Health Questionnaire, the Medical Coping Modes Questionnaire and the Multidimensional Scale of Perceived Social Support. Student's test, analysis of variance (ANOVA) and binary logistic regression analysis were used for data analysis.

Results: A total of 72.3% of participants reported psychological distress, and the prevalence of distress was higher in females (77.0%) than in males (68.8%). The usage of the acceptance-resignation coping style was positively related to psychological distress (B = 0.459,  < 0.001). Social support level was negatively associated with psychological distress (B = -0.049,  < 0.001). The use of the confrontation style was negatively related to psychological distress in females (OR = 0.87, 95% CI [0.78-0.96]) but not in males (OR = 0.98, 95% CI [0.91-1.06]) ( for interaction term = 0.007).

Conclusions: Greater use of the acceptance-resignation style and lower social support are related to a higher risk of psychological distress. Greater use of the confrontation style is related to a lower risk of psychological distress in females but not in males.
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http://dx.doi.org/10.7717/peerj.8713DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7103200PMC
March 2020

The Associations of Family Functioning, General Well-Being, and Exercise with Mental Health among End-Stage Renal Disease Patients.

Psychiatry Investig 2020 Apr 8;17(4):356-365. Epub 2020 Apr 8.

Department of Social Medicine and Health Management, School of Public Health, Jilin University, Changchun, China.

Objective: This study aims to explore the relationships of family functioning, general well-being, and exercise with psychological distress. Furthermore, we investigated the special roles of general well-being and exercise on the association between family functioning and psychological distress.

Methods: Of 769 end-stage renal disease (ESRD) patients participated in the cross-sectional study which consisted of the 12-item General Health Questionnaire (GHQ-12), the Family APGAR Scales, and the General Well-Being Schedule. The collected data were analyzed using multiple linear regression analysis and path analysis.

Results: The prevalence of psychological distress was 72.3%. Family functioning, general well-being and exercise were associated factors of psychological distress (p<0.05). The indirect effect of family functioning on psychological distress was partially mediated by general well-being (Effect=-0.08, 95% CI=-0.11, -0.04). In addition, the effect of family functioning on general well-being was moderated by exercise (Index=-0.092, SE=0.033, 95% CI=-0.159, -0.029).

Conclusion: The prevalence of psychological distress among ESRD patients was high. Family functioning, general well-being and exercise were associated with psychological distress. Family functioning could affect psychological distress partially by affecting general well-being. Furthermore, exercise had a significant moderating effect on the relationship between family functioning and general well-being.
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http://dx.doi.org/10.30773/pi.2019.0204DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7176562PMC
April 2020

Long non-coding RNA DDX11-AS1 facilitates gastric cancer progression by regulating miR-873-5p/SPC18 axis.

Artif Cells Nanomed Biotechnol 2020 Dec;48(1):572-583

Department of Digestive Diseases, Henan University Huaihe Hospital, Kaifeng, China.

Gastric cancer (GC) is a malignant tumour with high lethality. Accruing evidence elucidates the critical adjusting role of long non-coding RNA (lncRNAs) in human cancers. DDX11 antisense RNA 1 (DDX11-AS1) was previously found to be involved in GC pathogenesis. However, the precise molecular mechanisms of DDX11-AS1 need to be further investigated. In this study, we found that DDX11-AS1 expression was up-regulated in GC tumour tissues and cells. Increased DDX11-AS1 expression was associated with advanced TNM stage and lymph node metastasis. Functionally, knockdown of DDX11-AS1 repressed cell proliferation and clone formation, while induced cell cycle arrest and apoptosis. As expected, DDX11-AS1 overexpression displayed the opposite effect. Mechanically, DDX11-AS1 enhanced SPC18 expression through acting as a ceRNA for miR-873-5p. Furthermore, the inhibitory effect of DDX11-AS1 silencing on malignant biological behaviour of GC cells was attenuated by either miR-873-5p inhibitor or SEC11A up-regulation. Moreover, suppression of DDX11-AS1 also decreased GC tumorigenesis . In conclusion, DDX11-AS1 may serve as an oncogene in GC progression by sponging miR-873-5p and promoting SPC18 expression, providing a new insight into the mechanisms of DDX11-AS1 and elucidating a promising therapy target in GC.
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http://dx.doi.org/10.1080/21691401.2020.1726937DOI Listing
December 2020

Threshold-Tunable, Spike-Rate-Dependent Plasticity Originating from Interfacial Proton Gating for Pattern Learning and Memory.

ACS Appl Mater Interfaces 2020 Feb 29;12(6):7833-7839. Epub 2020 Jan 29.

State Key Laboratory of ASIC and System, Shanghai Institute of Intelligent Electronics & Systems, School of Microelectronics , Fudan University , Shanghai 200433 , People's Republic of China.

Recently, neuromorphic devices have been receiving increasing interest in the field of artificial intelligence (AI). Realization of fundamental synaptic plasticities on hard-ware devices would endow new intensions for neuromorphic devices. Spike-rate-dependent plasticity (SRDP) is one of the most important synaptic learning mechanisms in brain cognitive behaviors. It is thus interesting to mimic the SRDP behaviors on solid-state neuromorphic devices. In the present work, nanogranular phosphorus silicate glass (PSG)-based proton conductive electrolyte-gated oxide neuromorphic transistors have been proposed. The oxide neuromorphic transistors have good transistor performances and frequency-dependent synaptic plasticity behavior. Moreover, the neuromorphic transistor exhibits SRDP activities. Interestingly, by introducing priming synaptic stimuli, the modulation of threshold frequency value distinguishing synaptic potentiation from synaptic depression is realized for the first time on an electrolyte-gated neuromorphic transistor. Such a mechanism can be well understood with interfacial proton gating effects of the nanogranular PSG-based electrolyte. Furthermore, the effects of SRDP learning rules on pattern learning and memory behaviors have been conceptually demonstrated. The proposed neuromorphic transistors have potential applications in neuromorphic engineering.
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http://dx.doi.org/10.1021/acsami.9b22369DOI Listing
February 2020

Forensic genetic polymorphisms and population structure of the Guizhou Bouyei people based on 19 X-STR loci.

Ann Hum Biol 2019 Dec 3;46(7-8):574-580. Epub 2019 Dec 3.

Department of History, Department of Anthropology and Ethnology, Institute of Anthropology, National Institute for Data Science in Health and Medicine, Xiamen University, Xiamen, China.

Guizhou province is located in southwest China with abundant genetic, linguistic and cultural diversity. The Bouyei is one of the 18 officially recognised minority groups in Guizhou, accounting for about 97% of the total Bouyei population in China. However, the genetic history and forensic characterisation of the Bouyei people is largely unknown due to a lack of genetic data. We aim to investigate genetic polymorphisms and forensic characterisation of the Guizhou Bouyei population, as well as the relationships between the Bouyei and other East Asian populations. We genotyped 19 X-STRs in 188 males and 165 females of Guizhou Bouyei using the AGCU X19 STR Kit. We estimated allele frequencies, forensic parameters and genetic distances between the Bouyei and other East Asian populations. We presented the genetic distances in a phylogenetic tree, an MDS plot and a PCA plot. In Guizhou Bouyei individuals, we observed 216 alleles with corresponding frequencies ranging from 0.0019 to 0.6757. All of the six combined powers of PD, PD, MEC Krüger, MEC Kishida, MEC Desmarais and MEC Desmarais in allele diversity and haplotype diversity are larger than 0.99999995. We found genetic affinities among the Bouyei people and their geographical neighbouring populations in Guizhou, such as the Sui, Miao and Han. The highly polymorphic and informative forensic parameters of the 19 X-STRs in Bouyei people show the powerful potential of those markers in forensic identification and parentage tests. The genetic relationships of the Bouyei with other East Asian populations correspond well with geographic affiliations as well as linguistic classifications.
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http://dx.doi.org/10.1080/03014460.2019.1697362DOI Listing
December 2019

The mitochondrial DNA control region sequences from the Chinese Miao population of southeastern China.

Ann Hum Biol 2019 Dec 27;46(7-8):606-609. Epub 2019 Nov 27.

Department of Forensic Medicine, Guizhou Medical University, Guiyang, PR China.

Miao people are an officially recognised ethnic group living in southwest China, but have seldom been studied genetically, especially with respect to mtDNA data. To investigate the sequences and haplogroups of the mtDNA control region in a typical Miao population, with the aim of providing a good start for the expansion of the East Asian mtDNA reference database for forensic DNA analysis. We analysed 203 Miao individuals, looking at mtDNA control region sequences. We calculated and illustrated the haplotype frequencies, haplogroup distribution and pairwise Fst values between the Miao and six other worldwide populations to explore genetic polymorphisms and population relationships. We observed 121 haplotypes with corresponding frequencies ranging from 0.0049 to 0.0690 in the Miao population. All the samples were assigned to 71 different haplogroups. The haplotype diversity and the random match probability were estimated to be 0.9844 and 0.0204, respectively. The pairwise Fst values and associated values among seven populations suggest that the Miao population has significant differences to the other six populations, and is relatively isolated compared with them. Our results suggest that frequency estimates for mtDNA haplotypes in Miao ethnic groups should be determined independently rather than being pooled with other populations.
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http://dx.doi.org/10.1080/03014460.2019.1694701DOI Listing
December 2019

Population genetic analysis of 36 Y-chromosomal STRs yields comprehensive insights into the forensic features and phylogenetic relationship of Chinese Tai-Kadai-speaking Bouyei.

PLoS One 2019 8;14(11):e0224601. Epub 2019 Nov 8.

Department of Forensic Medicine, Guizhou Medical University, Guiyang, Guizhou, China.

Male-specifically inherited Y-STRs, harboring the features of haploidy and lack of crossing over, have gained considerable attention in population genetics and forensic investigations. Goldeneye® Y-PLUS kit was a recently developed amplification system focused on the genetic diversity of 36 Y-chromosomal short tandem repeats (Y-STRs) in East Asians. However, no population data and corresponding forensic features were reported in China. Here, 36 Y-STRs were first genotyped in 400 unrelated healthy Tai-Kadai-speaking Bouyei male individuals. A total of 371 alleles and 396 haplotypes could be detected, and the allelic frequencies ranged from 0.0025 to 0.9875. The haplotype diversity, random match probability and discrimination capacity values were 0.9999, 0.0026 and 0.9900, respectively. The gene diversity (GD) of 36 Y-STR loci in the studied group ranged from 0.0248 (DYS645) to 0.9601 (DYS385a/b). Population comparisons between the Guizhou Bouyei and 80 reference groups were performed via the AMOVA, MDS, and phylogenetic relationship reconstruction. The results showed that the population stratification was almost consistent with the geographic distribution and language-family, both among Chinese and worldwide ethnic groups. Our newly genotyped Bouyei samples show a close affinity with other Tai-Kadai-speaking groups in China and Southeast Asia. Our data may provide useful information for paternal lineage in the forensic application and population genetics, as well as evidence for archaeological and historical research.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0224601PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6839857PMC
March 2020

Comparison of Billroth I, Billroth II, and Roux-en-Y Reconstruction After Totally Laparoscopic Distal Gastrectomy: A Randomized Controlled Study.

Adv Ther 2019 11 12;36(11):2997-3006. Epub 2019 Oct 12.

Department of General Surgery, Renmin Hospital of Wuhan University, Wuhan, China.

Introduction: This study aimed to explore the efficacy of delta-shaped Billroth I anastomosis in totally laparoscopic distal gastrectomy for digestive tract reconstruction, and provide clinical data for determining the most appropriate digestive tract reconstruction method after distal gastrectomy.

Methods: This was a prospective randomized controlled study. A total of 180 patients were randomly and prospectively selected from Renmin Hospital of Wuhan University by random number table. These patients were randomly divided into three groups: Billroth I group, Billroth II group, and Roux-en-Y (RY) group.

Results: There were significant differences in resection margin, blood nutrition, and the number of postoperative complications among these three groups (P < 0.05). Furthermore, the resection margin, blood nutrition status, and immunization of patients in these three groups were determined. Compared to the other groups, the RY group was better in terms of hematologic status, immunological index, and postoperative complications.

Conclusion: Delta-shaped Billroth I anastomosis in totally laparoscopic distal gastrectomy for digestive tract reconstruction is simple and easy to perform, and has an advantage in postoperative gastrointestinal function recovery. RY reconstruction is superior to Billroth I and Billroth II in terms of postoperative complications.
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http://dx.doi.org/10.1007/s12325-019-01104-3DOI Listing
November 2019

Risk of natalizumab-associated PML in patients with MS is reduced with extended interval dosing.

Neurology 2019 10 12;93(15):e1452-e1462. Epub 2019 Sep 12.

From the Department of Neurology (L.Z.R., I.K.), NYU Langone Health, New York University, New York; Rocky Mountain MS Clinic (J.F., R.R.M.), Salt Lake City, UT; Biogen (I.C., E.R., K.S., R.K., Z.R., C.H., P-R.H., N.C.), Cambridge, MA; University of Alabama School of Public Health (G.C.), Birmingham; and Division of Biostatistics (J.D.G., X.L.), New York University School of Medicine, New York.

Objective: To use the large dataset from the Tysabri Outreach: Unified Commitment to Health (TOUCH) program to compare progressive multifocal leukoencephalopathy (PML) risk with natalizumab extended interval dosing (EID) vs standard interval dosing (SID) in patients with multiple sclerosis (MS).

Methods: This retrospective cohort study included anti-JC virus antibody-positive patients (n = 35,521) in the TOUCH database as of June 1, 2017. The effect of EID on PML risk was evaluated with 3 planned analyses using Kaplan-Meier methods stratified by prior immunosuppressant use. Risk of PML was analyzed by Cox regression adjusted for age, sex, prior immunosuppressants, time since natalizumab initiation, and cumulative number of infusions.

Results: This study included 35,521 patients (primary analysis: 1,988 EID, 13,132 SID; secondary analysis: 3,331 EID, 15,424 SID; tertiary analysis: 815 EID, 23,168 SID). Mean average dosing intervals were 35.0 to 43.0 and 29.8 to 30.5 days for the EID and SID cohorts, respectively. Hazard ratios (95% confidence intervals) of PML risk for EID vs SID were 0.06 (0.01-0.22, < 0.001) and 0.12 (0.05-0.29, < 0.001) for the primary and secondary analyses, respectively. Relative risk reductions were 94% and 88% in favor of EID for the primary and secondary analyses, respectively. The tertiary analysis included no cases of PML with EID.

Conclusion: Natalizumab EID is associated with clinically and statistically significantly lower PML risk than SID.

Classification Of Evidence: This study provides Class III evidence that for patients with MS, natalizumab EID is associated with a lower PML risk than SID.
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http://dx.doi.org/10.1212/WNL.0000000000008243DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7010325PMC
October 2019

Long non-coding RNA Malat1 activated autophagy, hence promoting cell proliferation and inhibiting apoptosis by sponging miR-101 in colorectal cancer.

Cell Mol Biol Lett 2019 27;24:50. Epub 2019 Jul 27.

1Department of Gastroenterology, Huaihe Hospital, Henan University, Kaifeng, 475000 Henan China.

Background: Long non-coding RNA Malat1 has been widely identified as an oncogene which shows a significant relationship with tumorigenesis in colorectal cancer (CRC). Nonetheless, whether Malat1 participates in the autophagy of colorectal cancer remains unclear.

Materials And Methods: First, the expression level of Malat1 in 96 pairs of colorectal cancer tissues and four cell lines was detected by qRT-PCR. Subsequently, the autophagy activity in colorectal cancer tissues and cell lines was detected by western blot. Furthermore, the CCK-8 assay and flow cytometry (FCM) were performed to detect the role of autophagy activated by Malat1 in colorectal cancer cell lines.

Results: In this study, significantly increased Malat1 expression and autophagy activity were found in colorectal cancer tissues compared with the adjacent normal tissues. Also, the Malat1 level was positively correlated with the expression of LC3-II mRNA in vivo. Moreover, autophagy activation and cell proliferation were significantly facilitated by Malat1 in colorectal cancer cells, while apoptosis decreased. Above all, the inhibition of autophagy by 3-MA not only relieved the Malat1-induced cell proliferation but also promoted the Malat1-induced cell apoptosis. In addition, Malat1 was found to act as an endogenous sponge by directly binding to miR-101 to reduce miR-101. Furthermore, the suppressive effects of miR-101 on the autophagy, proliferation, and apoptosis of CRC were abolished by Malat1.

Conclusion: Long non-coding RNA Malat1 activated autophagy and promoted cell proliferation, yet inhibited apoptosis by sponging miR-101 in colorectal cancer cells.
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http://dx.doi.org/10.1186/s11658-019-0175-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6660674PMC
April 2020

Associations of family relationships and negative life events with depressive symptoms among Chinese adolescents: A cross-sectional study.

PLoS One 2019 18;14(7):e0219939. Epub 2019 Jul 18.

Department of Social Medicine and Health Management, School of Public Health, Jilin University, Changchun, China.

Objectives: The main objective of this study was to explore the associations of family relationships and negative life events with depressive symptoms among Chinese adolescents.

Methods: A cross-sectional study of 3081 middle school students was conducted in Ganzhou City, Jiangxi Province, China. Students were asked to complete questionnaires regarding family relationships, negative life events, and depressive symptoms. A mediation analysis was carried out using a multiple regression analysis and the PROCESS macro method.

Results: Of all participants, 19.9% reported experiencing depressive symptoms. The prevalence of depressive symptoms was 13.0% and 29.2% in participants with good and poor parental relationships, and the prevalence of depressive symptoms was 11.4% and 30.9% in participants with closed and alienated parental-child relationships, respectively. Parental relationships, parental-child relationships, and negative life events were positively correlated with depressive symptoms. The effect of parental relationships on depressive symptoms was fully mediated by negative life events (Effect = 0.052, 95% CI = [0.023, 0.082]), while the effect of parent-child relationships on adolescent depressive symptoms was partially mediated by negative life events (Effect = 0.075, 95% CI = [0.048, 0.104]).

Conclusions: Our results showed a high prevalence of depressive symptoms in Chinese adolescents. Poor family relationships may have the potential to increase the risk of depressive symptoms, and they could affect depressive symptoms through negative life events.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0219939PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6638951PMC
March 2020
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