Publications by authors named "Zheng Cao"

353 Publications

Is robotic-assisted technology still accurate in total hip arthroplasty for fibrous-fused hips?

J Arthroplasty 2022 Aug 5. Epub 2022 Aug 5.

Senior Department of Orthopedics, the Forth Medical Center of Chinese PLA General Hospital, No.51 Fucheng Road, Haidian District, Beijing, China; National Clinical Research Center for Orthopedics, Sports Medicine and Rehabilitation, No.28 Fuxing Road, Haidian District, Beijing, China. Electronic address:

Background: Total hip arthroplasty (THA) for fibrous-fused hips is technically demanding. This study aimed to evaluate the precision and accuracy, as well as rate of conversion of robotic-assisted THA in such difficult patients.

Methods: We retrospectively analyzed 67 patients (84 hips) who underwent THA with fibrous-fused hips between August 2018 and June 2021 at our institution. Demographics, acetabular cup positionings, leg length discrepancies (LLDs), and postoperative Harris Hip Scores (HHS) were recorded for all patients. Thirty-six patients (44 hips) who underwent robotic-assisted THA and 31 patients (40 hips) who underwent manual THA were enrolled in this study.

Results: The robot accurately executed the preoperative plan, and there were no statistically significant differences between the preoperative planned anteversion, inclination, and the postoperative measurements. In the robotic group, the percentage of acetabular cups in the safety zone was significantly higher than in the manual group (87.2 vs 55%, respectively, p=0.042). The rate of conversion to manual THA for various reasons in the robotic-assisted THA group was 11.4% (5/44). Compared with manual THA, the mean increase in operative time for conversion from robotic-assisted to manual THA was 24 minutes (p<0.001).

Conclusions: In patients who have fibrous-fused hips, preoperative planning can be accurately executed by robotic-assisted technology. Compared with manual THA, robotic-assisted THA had a significant advantage in improving the frequency of achieving cup positioning within the target zone. Overall, robotic-assisted technology was helpful in such difficult cases, and the approximately 11.4% of cases converted to manual THA are reminders that surgeons should be thoroughly prepared preoperatively.
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http://dx.doi.org/10.1016/j.arth.2022.07.023DOI Listing
August 2022

Empagliflozin inhibits macrophage inflammation through AMPK signaling pathway and plays an anti-atherosclerosis role.

Int J Cardiol 2022 Aug 2. Epub 2022 Aug 2.

Hubei University of Medicine, Shiyan 442000, Hubei, China; Taihe Hospital, Shiyan 442000, Hubei, China. Electronic address:

Objective: In recent years, some authoritative clinical studies have found that SGLT2 inhibitor can reduce cardiovascular risk in patients with diabetes, which may imply that SGLT2 inhibitor can play a role beyond lowering blood glucose. In this study, we explored the effect of empagliflozin on vascular atherosclerosis after removing the effect of diabetes.

Methods: The interaction between SGLT2 inhibitor and the AMPK(Adenosine 5'-monophosphate-activated protein kinase) signal pathway to attenuate atherosclerosis was studied in both spontaneously atherosclerotic mice in vivo and oxidized low-density lipoprotein(ox-LDL) induced macrophage inflammation model in vitro. In vivo experiment the aorta tree and aortic valve area were stained with oil red, and the level of inflammatory factors in the diseased tissue was evaluated by immunohistochemistry. Meanwhile, serum was collected to detect the levels of inflammatory factors. In vitro experiment, the RAW264.7 cell line was selected and ox-LDL was used to induce the release of proinflammatory factors, and different doses of empagliflozin were added. The phagocytosis of macrophages to ox-LDL density lipoprotein, and the expression of inflammatory factors at the protein and RNA levels were measured.

Results: Empagliflozin reduced the area of atherosclerotic plaque and macrophage infiltration in atherosclerotic plaques, decreased the expression of inflammatory factors in local plaque tissues and serum of APOE mice fed with high-fat diet. Empagliflozin can improve the protein expression level of p-AMPK affected by ox-LDL in cell and reduce the gene expression level of inflammatory factors and protein expression level of NF-κB, thus playing an anti-atherosclerosis role.

Conclusions: Empagliflozin improves energy metabolism and reduces the expression of inflammatory factors by activating AMPK. As empagliflozin inhibits atherosclerosis progression, it may be of use in prevention of cardiovascular diseases.
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http://dx.doi.org/10.1016/j.ijcard.2022.07.048DOI Listing
August 2022

Robotic-assisted versus manual total hip arthroplasty in obese patients: a retrospective case-control study.

J Orthop Surg Res 2022 Jul 30;17(1):368. Epub 2022 Jul 30.

Senior Department of Orthopedics, the Forth Medical Center of Chinese PLA General Hospital, No. 51 Fucheng Road, Haidian District, Beijing, 100853, China.

Aims: The objective of this study was to evaluate the accuracy of acetabular cup positioning in the obese patients when using robotic-assisted technology during total hip arthroplasty (THA).

Methods: Data were retrospectively collected from patients who underwent primary (THA) with a body mass index (BMI) ≥ 28 kg/m and ≥ 1 year of follow-up between January 2018 and December 2019. Their demographics, diagnosis, acetabular cup positioning, American Society of Anesthesiologists (ASA) score, Harris Hip Score (HHS), and Forgotten Joint Score (FJS) at the final follow-up were recorded for analysis.

Results: There were no statistically significant differences between the two groups in height, weight, BMI, ASA score, or preoperative Harris Hip Score (HHS). Also, there was no difference in inclination angle between the two groups (R-THA: 41.29° ± 3.04°; manual THA (M-THA): 40.47° ± 5.46°; P = 0.312). However, the mean anteversion angle was greater in the R-THA group (20.71° ± 1.98° vs. 19.08° ± 4.04°; P < 0.001). Compared to M-THA, R-THA more frequently achieved an acetabular cup angle within 5° of the target (anteversion, 98.1% vs. 78.1% P = 0.001; inclination, 88.5% vs. 53.1%, P < 0.001). The R-THA group was more advantageous in restoring the hip center of rotation (COR) and leg length difference (LLD). There was no statistical difference in postoperative HHS (P = 0.404) or FJS (P = 0.497) between the two groups.

Conclusions: Compared to manual technique, robotic-assisted technique provided more precise acetabular cup positioning and better leg length restoration for obese patients. The robotic-assisted technique was more advantageous in recovering the center of rotation position and achieved a higher proportion of the acetabular cup placed in the target safety zone. Further studies are needed to confirm the clinical outcomes of surgeries in obese patients using robotic-assisted technology.
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http://dx.doi.org/10.1186/s13018-022-03263-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338672PMC
July 2022

The Relationship between Intimate Partner Violence and Online Help-Seeking: A Moderated Mediation Model of Emotion Dysregulation and Perceived Anonymity.

Int J Environ Res Public Health 2022 Jul 7;19(14). Epub 2022 Jul 7.

Faculty of Agribusiness and Commerce, Lincoln University, Lincoln 7647, New Zealand.

During the COVID-19 pandemic, lockdowns and isolation have limited the availability of face-to-face support services for victims of intimate partner violence (IPV). Despite the growing need for online help in supporting IPV victims, far less is known about the underlying mechanisms between IPV and online help-seeking. We studied the mediating role of emotion dysregulation (ED) and the moderating role of perceived anonymity (PA) on the internet to explain IPV victims' willingness of online help-seeking (WOHS). Through a PROCESS analysis of the questionnaire data ( = 510, 318 female, 192 male, M = 22.41 years), the results demonstrate that: (1) ED has been linked with the experience of IPV, and IPV significantly induces ED. (2) When IPV victims realize the symptoms of ED, they have a strong willingness to seek external intervention to support themselves. ED mediates the relationship between IPV and online help-seeking. (3) For youth growing up in the era of social networking sites (SNS), personal privacy protection is an important factor when seeking online help. The anonymity of the internet has a positive effect on victims who experience IPV and ED, and it increases WOHS. This study introduces a new perspective on the psychological mechanism behind IPV victims' help-seeking behaviors, and it suggests that the improvement of anonymity in online support can be an effective strategy for assisting IPV victims.
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http://dx.doi.org/10.3390/ijerph19148330DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319494PMC
July 2022

Meis1 Regulates Nociceptor Development and Behavioral Response to Tactile Stimuli.

Front Mol Neurosci 2022 6;15:901466. Epub 2022 Jul 6.

Beijing Institute of Biotechnology, Beijing, China.

Nociceptors in the dorsal root ganglia (DRG) and trigeminal ganglia (TG) are necessary for transmitting pain and itch signals. However, the molecular mechanism regulating nociceptor development remains largely unknown. This study identifies that the transcription factor is generally expressed in two groups of sensory neurons in the developing DRG. During prenatal and neonatal stages, approximately 2/3 of Meis1+ neurons are Runx1+ nociceptors, while 1/3 of Meis1+ neurons are NF200+ myelinated neurons. At postnatal stages, Meis1 expression in nociceptors is gradually reduced. Here, we constructed a conditional knockout mouse line to selectively delete in Nav1.8 lineage nociceptors. Microarray analyses showed that differentially expressed genes in the mutant DRG were enriched in pathways related to sensory perception of pain and nervous system development. In addition, regulates the expression of some marker genes of Nppb+ neurons and C-LTMRs. Furthermore, mutant mice exhibit behavioral deficits in response to light mechanical pain, static touch and chemical itch. Therefore, this study reveals that is required to regulate the development of nociceptors.
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http://dx.doi.org/10.3389/fnmol.2022.901466DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9301487PMC
July 2022

Real-Time Quantification of Cell Internalization Kinetics by Bioluminescent Probes.

Methods Mol Biol 2022 ;2525:93-107

Department of Microbiology, Immunology and Molecular Genetics, David Geffen School of Medicine, UCLA AIDS Institute, University of California, Los Angeles, Los Angeles, CA, USA.

Alongside the intracellular transport of nutrients needed for cellular homeostasis, great efforts exist to effectively deliver substances such as proteins and genes into the cell for therapy, gene editing, disease diagnosis, and more. To evaluate the intracellular delivery of such substances, conventional methods impose semi-quantifications and discrete measures of the dynamic process of cellular internalization. Herein, we detail the methods to quantify cell internalization kinetics in real-time using individually nano-encapsulated bioluminescent Firefly Luciferase (FLuc) enzymes as probes. We include a comprehensive protocol to synthesize and characterize the encapsulated FLuc, assay the real-time bioluminescence (BL) in cells, and analyze the real-time BL profile to extract key parameters of cell internalization kinetics. Quantifying the kinetics of intracellular delivery offers the opportunity to resolve the underlying mechanisms governing membrane translocation and provide measures reflecting cellular state and metabolism while playing a critical role in the clinical development of effective vectors.
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http://dx.doi.org/10.1007/978-1-0716-2473-9_7DOI Listing
July 2022

Icariside I - A novel inhibitor of the kynurenine-AhR pathway with potential for cancer therapy by blocking tumor immune escape.

Biomed Pharmacother 2022 Jul 11;153:113387. Epub 2022 Jul 11.

State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Centre for Magnetic Resonance in Wuhan, Innovation Academy of Precision Measurement Science and Technology, CAS, Wuhan 430071, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

Background: Although therapeutic antibodies against immune checkpoints such as PD-1/PD-L1 have achieved unprecedented success in clinical tumor patients, there are still many patients who are ineffective or have limited responses to immune checkpoint blockade (ICB). Discovery of novel strategies for cancer immunotherapy including natural small molecules is needed.

Methods: Owing to its extremely low content in Epimedium genus, we firstly constructed a microbial cell factory to enzymatically biosynthesize icariside I, a natural flavonoid monosaccharide from Herbal Epimedium. Using a combination of targeted MS-based metabolomics, flow cytometric analysis, and biological assays, the therapeutic potentials of icariside I were subsequently investigated in vivo and in vitro.

Results: We find that icariside I markedly downregulates a series of intermediate metabolites such as kynurenine, kynurenic acid and xanthurenic acid and corresponding key enzymes involved in kynurenine-AhR pathway in both tumor cells and tumor-bearing mice. In vivo, oral administration of icariside I downregulates SLC7A8 and PAT4 transporters and AhR, thus inhibiting nuclear PD-1 in CTLs. Moreover, icariside I significantly upregulates CD8 + T cells in both peripheral blood and tumor tissues of tumor-bearing mice. Consequently, interferon-γ (IFN-γ) secreted by CD8 + T cells suppresses tumor growth through activation of JAK1-STAT1 signaling, thus inducing tumor cell apoptosis.

Conclusions: These results suggest that icariside I could be an effective small molecule drug for tumor immunotherapy by blocking kynurenine-AhR pathway and tumor immune escape.
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http://dx.doi.org/10.1016/j.biopha.2022.113387DOI Listing
July 2022

Curcumin mitigates deoxynivalenol-induced intestinal epithelial barrier disruption by regulating Nrf2/p53 and NF-κB/MLCK signaling in mice.

Food Chem Toxicol 2022 Sep 8;167:113281. Epub 2022 Jul 8.

Key Laboratory of the Provincial Education, Department of Heilongjiang for Common Animal Disease Prevention and Treatment, College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China. Electronic address:

Deoxynivalenol (DON) induces intestinal epithelial barrier disruption, posing a threat to the body. Curcumin (Cur) possesses pharmacological bioactivities such as antioxidant and anti-inflammatory effects that help maintain intestinal health. Here, the protective effects of Cur against DON-induced intestinal epithelial barrier disruption were explored. Cur (75 or 150 mg/kg body weight [B.W.]) alleviated DON-induced (2.4 mg/kg B.W.) inhibition of growth performance and morphological damage to intestinal epithelium in mice. Cur also significantly attenuated DON-induced intestinal epithelial barrier disruption and structural damage to the tight junctions (TJs), as assessed by ultrastructure observation, serum FITC-dextran concentrations and diamine oxidase activity. Cur mitigated the DON-induced increase in reactive oxygen species, malondialdehyde and 8-hydroxy-2'-deoxyguanosine levels; p53, cytoplasmic cytochrome c, Bax, and Bcl-2 expression; and TUNEL-positive cell rate and caspase-3 activity. It decreased the total antioxidant capacity and expression of nuclear Nrf2 and its downstream target genes. Lastly, Cur attenuated the DON-induced increase in MLCK, p-MLC, nuclear NF-κB p65 expression, and the NF-κB downstream target genes; decrease in the expression of TJs proteins (claudin-1, occludin, and zonula occludens-1 [ZO-1]); and abnormal ZO-1 distribution. Overall, Cur mitigated the DON-induced disruption of the intestinal epithelial barrier by regulating the Nrf2/p53-mediated apoptotic pathway and NF-κB/MLCK-mediated TJs pathway in mice.
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http://dx.doi.org/10.1016/j.fct.2022.113281DOI Listing
September 2022

KRAS-G12D mutation drives immune suppression and the primary resistance of anti-PD-1/PD-L1 immunotherapy in non-small cell lung cancer.

Cancer Commun (Lond) 2022 Jul 11. Epub 2022 Jul 11.

Department of Thoracic Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, P. R. China.

Background: Although immune checkpoint inhibitors (ICIs) against programmed cell death protein 1 (PD-1) and its ligand PD-L1 have demonstrated potency towards treating patients with non-small cell lung carcinoma (NSCLC), the potential association between Kirsten rat sarcoma viral oncogene homolog (KRAS) oncogene substitutions and the efficacy of ICIs remains unclear. In this study, we aimed to find point mutations in the KRAS gene resistant to ICIs and elucidate resistance mechanism.

Methods: The association between KRAS variant status and the efficacy of ICIs was explored with a clinical cohort (n = 74), and confirmed with a mouse model. In addition, the tumor immune microenvironment (TIME) of KRAS-mutant NSCLC, such as CD8 tumor-infiltrating lymphocytes (TILs) and PD-L1 level, was investigated. Cell lines expressing classic KRAS substitutions were used to explore signaling pathway activation involved in the formation of TIME. Furthermore, interventions that improved TIME were developed to increase responsiveness to ICIs.

Results: We observed the inferior efficacy of ICIs in KRAS-G12D-mutant NSCLC. Based upon transcriptome data and immunostaining results from KRAS-mutant NSCLC, KRAS-G12D point mutation negatively correlated with PD-L1 level and secretion of chemokines CXCL10/CXCL11 that led to a decrease in CD8 TILs, which in turn yielded an immunosuppressive TIME. The analysis of cell lines overexpressing classic KRAS substitutions further revealed that KRAS-G12D mutation suppressed PD-L1 level via the P70S6K/PI3K/AKT axis and reduced CXCL10/CXCL11 levels by down-regulating high mobility group protein A2 (HMGA2) level. Notably, paclitaxel, a chemotherapeutic agent, upregulated HMGA2 level, and in turn, stimulated the secretion of CXCL10/CXCL11. Moreover, PD-L1 blockade combined with paclitaxel significantly suppressed tumor growth compared with PD-L1 inhibitor monotherapy in a mouse model with KRAS-G12D-mutant lung adenocarcinoma. Further analyses revealed that the combined treatment significantly enhanced the recruitment of CD8 TILs via the up-regulation of CXCL10/CXCL11 levels. Results of clinical study also revealed the superior efficacy of chemo-immunotherapy in patients with KRAS-G12D-mutant NSCLC compared with ICI monotherapy.

Conclusions: Our study elucidated the molecular mechanism by which KRAS-G12D mutation drives immunosuppression and enhances resistance of ICIs in NSCLC. Importantly, our findings demonstrate that ICIs in combination with chemotherapy may be more effective in patients with KRAS-G12D-mutant NSCLC.
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http://dx.doi.org/10.1002/cac2.12327DOI Listing
July 2022

PINK1/Parkin-mediated mitophagy is activated to protect against AFB-induced immunosuppression in mice spleen.

Toxicol Lett 2022 Aug 8;366:33-44. Epub 2022 Jul 8.

Key Laboratory of the Provincial Education, Department of Heilongjiang for Common Animal Disease Prevention and Treatment, College of Veterinary Medicine, Northeast Agricultural. University, Harbin 150030, China. Electronic address:

Aflatoxin B (AFB) can cause mitochondrial malfunction and immunosuppression in spleen. Mitochondrial damage can lead to oxidative stress and aggravate immune cell dysfunction. Phosphatase and tensin homolog (PTEN)-induced putative kinase1 (PINK1)/ E3 ubiquitin ligase PARK2 (Parkin)-mediated mitophagy can scavenge damaged mitochondria and alleviate oxidative stress to maintain cellular homeostasis. However, the role of PINK1/Parkin-mediated mitophagy in AFB-induced immunosuppression in spleen is unclear. In this study, sixty male mice were sensibilized orally with AFB at different concentrations [0, 0.5, 0.75, and 1 mg/kg body weight (BW)] for 28 days, and AFB caused splenic structure injury and immunosuppression, also led to upregulation of PINK1/Parkin-mediated mitophagy in a dose-dependent manner. Subsequently, thirty male WT C57BL/6 N mice and thirty male Parkin knockout (Parkin) C57BL/6 N mice were sensibilized orally with AFB at 0 or 1 mg/kg BW for 28 days, and Parkin inhibited mitophagy and further aggravated AFB-induced splenic structure injury, immunosuppression, mitochondrial damage and oxidative stress. Collectively, these results indicate that AFB exposure activates PINK1/Parkin-mediated mitophagy, which protects against immunosuppression in spleen.
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http://dx.doi.org/10.1016/j.toxlet.2022.07.001DOI Listing
August 2022

Revision Total Hip Arthroplasty with Severe Acetabular Defect: A Preliminary Exploration and Attempt of Robotic-Assisted Technology.

Orthop Surg 2022 Jul 6. Epub 2022 Jul 6.

Senior Department of Orthopedics, The Forth Medical Center of Chinese PLA General Hospital, Beijing, China.

Background: Robotic-assisted technology may be useful in hip revision cases with acetabular defects. However, data on the use of robotic-assisted technology for such complex diseases is lacking.

Case Presentation: This case study described the adoption of MAKO robotic-assisted treatment of revision total hip arthroplasty (THA) combined with severe acetabular defect (Paprosky type IIIB). Robotic-assisted technology accurately achieved preoperative planning; the acetabular component and augment were placed in the original position and angle as planned. Robotic-assisted acetabular reaming was successful in a single pass, preserving the remaining acetabular bone mass very well with no procedure-related complications. The Harris Hip Score (HHS) at 6 months postoperatively was 84 and the Western Ontario and McMaster Universities (WOMAC) Osteoarthritis Index was 24.

Conclusion: Robotic-assisted technology can help in the accurate reconstruction of acetabular defect in complex hip revision surgery.
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http://dx.doi.org/10.1111/os.13368DOI Listing
July 2022

Transcriptome analysis of IHHNV infection in Penaeus vannamei at different developmental stages.

Fish Shellfish Immunol 2022 Jun 26;127:329-339. Epub 2022 Jun 26.

School of Agriculture, Ludong University, Yantai, 264025, PR China. Electronic address:

Infectious hypodermal and haematopoietic necrosis virus (IHHNV) is the smallest known virus in shrimp, which causes runt-deformity syndrome (RDS) and leads to huge economic loss every year in penaeid shrimp farming. Previous studies have shown that the juvenile Penaeus vannamei is more susceptible to IHHNV infection than the adults, but the mechanism is still unclear. In order to investigate the mechanism of pathogenic differences in IHHNV infection of P. vannamei at different developmental stages, the juvenile and adult P. vannamei were studied by transcriptome high-throughput sequencing to analyze their response to IHHNV infection. GO and KEGG enrichment were analyzed to search for differentially expressed genes (DEGs) related to immunity, growth and metabolism. The results showed that many immune-related genes of the juvenile and adult P. vannamei responded differently to IHHNV infection. For the adult P. vannamei, the expression of most immune-related genes was significantly up-regulated, which means that a cellular defense response was triggered after IHHNV infection. However, most immune-related genes in juvenile P. vannamei were inhibited, indicating that the immune system of juvenile the P. vannamei is imperfect and makes it to be more susceptible to IHHNV. Similarly, the growth-related genes of P. vannamei were changed during IHHNV infection. For the juvenile P. vannamei, the growth-related genes were significantly down-regulated, which resulted in a growth hormone disorder and prevented the juvenile P. vannamei from growth. In the adult P. vannamei, most molting-related genes were significantly up-regulated, indicating that IHHNV infection leads the adult P. vannamei to early molting to eliminate pathogen in the body. Metabolic process data showed that energy metabolism pathway was affected when P. vannamei infected with IHHNV. The adult P. vannamei infected with IHHNV can cause energetically costly and lead to the disturbance of the metabolism, activate complex immune systems to resist the invasion of pathogens. The results of this study clarified the response mechanism of P. vannamei at different developmental stages to IHHNV infection, which can provide new insights to IHHNV effective control and a reference for the study of sensitive period of different shrimp virus to host infection.
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http://dx.doi.org/10.1016/j.fsi.2022.06.038DOI Listing
June 2022

Divergent Associations Between Serum Androgens and Ovarian Reserve Markers Revealed in Patients With Polycystic Ovary Syndrome.

Front Endocrinol (Lausanne) 2022 9;13:881740. Epub 2022 Jun 9.

Department of Laboratory Medicine, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing Maternal and Child Health Care Hospital, Beijing, China.

Background: The role of excess androgen in ovarian reserve remains unclear in patients with polycystic ovary syndrome (PCOS). Our study highlights the associations of serum androgen levels and ovarian reserve markers in PCOS and non-PCOS women.

Methods: Totally 584 menstrual abnormalities women of 20-45 years were retrospectively evaluated at the Beijing Obstetrics and Gynecology Hospital between January 2021 to October 2021. The enrolled patients were classified into two groups: the PCOS group (n=288) and the non-PCOS group (n=296) based on the Rotterdam consensus for PCOS diagnosis. The serum androgens, including testosterone (T), free testosterone (FT, calculated), bioavailable testosterone (Bio-T, calculated), androstenedione (A2), dihydrotestosterone (DHT), dehydroepiandrosterone (DHEA), and dehydroepiandrosterone sulfate (DHEAS) were assessed with an in-house developed liquid chromatography tandem mass spectrometry (LC-MS/MS) method. The associations between the serum androgens and the hormone markers commonly used for evaluating ovarian reserve function, such as anti-mullerian hormone (AMH) and the ratio of luteinizing hormone (LH)/follicle stimulating hormone (FSH) were explored.

Results: The serum T, FT, Bio-T, A2, DHT, DHEA, DHEAS, AMH and LH/FSH of the PCOS group were 51.7 ± 23.2 ng/dL/mL, 8.5 ± 5.0 pg/mL, 210.1 ± 127.7 pg/mL, 1.9 ± 0.8 ng/mL, 0.2 ± 0.1 ng/mL, 6.4 ± 4.2 ng/mL, 2431.0 ± 1030.7 ng/mL, 6.7 ± 3.8 ng/mL, and 1.8 ± 1.4 respectively, which were significantly higher than those in the non-PCOS group (<0.05). In the group of PCOS patients, T and A2 levels were positively associated with AMH in both multivariate linear regression analysis and Pearson's correlation analysis. Similar but weaker associations were observed in the non-PCOS patients. In the PCOS patients with hyperandrogenemia (HA), the AMH level was significantly higher in the subjects with T increased than in the subjects with non-T androgen(s) increased (A2, DHT, DHEA or DHEAS).

Conclusions: The serum androgen levels are positively associated with ovarian reserve markers in both of the PCOS and the non-PCOS patients in our study. In the PCOS group, the highest AMH level was observed in the subjects with the T elevation subgroup, suggesting that T is more closely related with the increase of AMH when compared with other androgens investigated.
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http://dx.doi.org/10.3389/fendo.2022.881740DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9218193PMC
June 2022

Full-Endoscopic Lumbar Interbody Fusion Versus Minimally Invasive Transforaminal Lumbar Interbody Fusion with a Tubular Retractor System: A Retrospective Controlled Study.

World Neurosurg 2022 Jun 22. Epub 2022 Jun 22.

Department of Orthopedic Surgery, the Fourth Medical Center of Chinese PLA General Hospital, Beijing, China.

Objective: The objective of this study was to compare the safety and clinical efficacy of full-endoscopic lumbar interbody fusion (FE-LIF) and minimally invasive transforaminal lumbar interbody fusion (MIS-TLIF).

Methods: A total of 70 patients with single-level lumbar degenerative diseases underwent FE-LIF or MIS-TLIF with a tubular retractor system from August 2018 to August 2020. Postoperatively, the efficacy and safety were compared using several clinical and radiological indices.

Results: A total of 32 patients underwent FE-LIF and 38 received MIS-TLIF with a tubular retractor system, and all patients had no apparent complications. The FE-LIF group had higher radiation exposure, longer operation time, and less bleeding than the MIS-TLIF group (P < 0.05). Postoperative lumbar magnetic resonance imaging showed that the nerve decompression was sufficient. The pain in the lower back and legs was significantly relieved, and the Oswestry Disability Index (ODI) score was greatly improved after surgery (P < 0.01) in both the groups. The sensory and motor functions of nerve roots were remarkably recovered in both the groups at the 1-year follow-up (P < 0.05), and there was no significant difference in MacNab scores between the 2 groups. As per Mannion's fusion classification, the interbody fusion rate was significantly better in the FE-LIF group than in the MIS-TLIF group.

Conclusions: FE-LIF, which is safe, effective, and minimally invasive, exhibits the same clinical efficacy as MIS-TLIF but with longer operation time and increased radiation exposure.
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http://dx.doi.org/10.1016/j.wneu.2022.06.083DOI Listing
June 2022

Effect of thermal treatment at high temperature on phase stability and transformation of YbO and YO co-doped ZrO ceramics.

Sci Rep 2022 Jun 15;12(1):9955. Epub 2022 Jun 15.

Inner Mongolia Key Laboratory of Advanced Ceramic Materialss and Devices, Inner Mongolia University of Science and Technology, 7 Arding Street, Baotou, 014010, China.

YO doped ZrO (YSZ) ceramic material is used to protect alloy components worked in high-temperature. But its phase transformation between tetragonal phase and monoclinic phase occurred at 1150 °C leads to YSZ invalid. Therefore, enhancing the phase stability of YSZ is necessary for meeting the demands of the development of thermal barrier coatings (TBC). In this study, X-ray diffraction and Raman spectra were used to explore the phase stability and phase transformation of YbO and YO co-doped ZrO (YbYSZ) ceramics after heat treatment at 1300 °C with different times. The stability of tetragonal phase is improved by tetragonality decreasing with Yb doped. Simultaneously, the incorporation of Yb leads to O-O coupling, which is beneficial for increasing the concentration of oxygen vacancies near the substituted ions, thereby improving the stability of the crystal. The 6.5YbYSZ ceramic has the best stability after heat treatment at 1300 °C for different times.
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http://dx.doi.org/10.1038/s41598-022-13705-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9200844PMC
June 2022

Characterization of triclosan-induced hepatotoxicity and triclocarban-triggered enterotoxicity in mice by multiple omics screening.

Sci Total Environ 2022 Sep 9;838(Pt 4):156570. Epub 2022 Jun 9.

State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Centre for Magnetic Resonance in Wuhan, Innovation Academy of Precision Measurement Science and Technology, Chinese Academy of Sciences (CAS), Wuhan 430071, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China. Electronic address:

Triclosan (2,4,4'-trichloro-2'-hydroxydiphenyl ether, TCS) and triclocarban (3,4,4'-trichloro-carbanilide, TCC) are two antimicrobial agents commonly used for personal care products. Previous studies primarily focused on respective harmful effects of TCS and TCC. In terms of their structural similarities and differences, however, the structure-toxicity relationships on health effects of TCS and TCC exposure remain unclear. Herein, global H NMR-based metabolomics was employed to screen the changes of metabolic profiling in various biological matrices including liver, serum, urine, feces and intestine of mice exposed to TCS and TCC at chronic and acute dosages. Metagenomics was also applied to analyze the gut microbiota modulation by TCS and TCC exposure. Targeted MS-based metabolites quantification, histopathological examination and biological assays were subsequently conducted to supply confirmatory information on respective toxicity of TCS and TCC. We found that oral administration of TCS mainly induced significant liver injuries accompanied with inflammation and dysfunction, hepatic steatosis fatty acids and bile acids metabolism disorders; while TCC exposure caused marked intestine injuries leading to striking disruption of colonic morphology, inflammatory status and intestinal barrier integrity, intestinal bile acids metabolism and microbial community. These comparative results provide novel insights into structure-dependent mechanisms of TCS-induced hepatotoxicity and TCC-triggered enterotoxicity in mice.
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http://dx.doi.org/10.1016/j.scitotenv.2022.156570DOI Listing
September 2022

Characterization of the Immune Cell Infiltration Landscape Uncovers Prognostic and Immunogenic Characteristics in Lung Adenocarcinoma.

Front Genet 2022 23;13:902577. Epub 2022 May 23.

Department of Thoracic Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

The immune cell infiltration in TME has been reported to be associated with prognosis and immunotherapy efficiency of lung cancers. However, to date, the immune infiltrative landscape of lung adenocarcinoma (LUAD) has not been elucidated yet. Therefore, this study aimed to identify a new transcriptomic-based TME classification and develop a risk scoring system to predict the clinical outcomes of patients with LUAD. We applied "CIBERSORT" algorithm to analyze the transcriptomic data of LUAD samples and classified LUAD into four discrete subtypes according to the distinct immune cell infiltration patterns. Furthermore, we established a novel predictive tool (TMEscore) to quantify the immune infiltration patterns for each LUAD patient by principal component analysis. The TMEscore displayed as a reliable and independent prognostic biomarker for LUAD, with worse survival in TMEscrore-high patients and better survival in TMEscrore-low patients in both TCGA and other five GEO cohorts. In addition, enriched pathways and genomic alterations were also analyzed and compared in different TMEscore subgroups, and we observed that high TMEscore was significantly correlated with more aggressive molecular changes, while the low TMEscore subgroup enriched in immune active-related pathways. The TMEscore-low subtype showed overexpression of PD-1, CTLA4, and associations of other markers of sensitivity to immunotherapy, including TMB, immunophenoscore (IPS) analysis, and tumor immune dysfunction and exclusion (TIDE) algorithm. Conclusively, TMEscore is a promising and reliable biomarker to distinguish the prognosis, the molecular and immune characteristics, and the benefit from ICIs treatments in LUAD.
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http://dx.doi.org/10.3389/fgene.2022.902577DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9168373PMC
May 2022

SARS-CoV-2 N Protein Antagonizes Stress Granule Assembly and IFN Production by Interacting with G3BPs to Facilitate Viral Replication.

J Virol 2022 06 2;96(12):e0041222. Epub 2022 Jun 2.

Beijing Institute of Biotechnology, Beijing, China.

SARS-CoV-2 is the causative agent of the ongoing pandemic of coronavirus disease 2019 (COVID-19) and poses a significant threat to global health. N protein (NP), which is a major pathogenic protein among betacoronaviruses, binds to the viral RNA genome to allow viral genome packaging and viral particle release. Recent studies showed that NP antagonizes interferon (IFN) induction and mediates phase separation. Using live SARS-CoV-2 viruses, this study provides solid evidence showing that SARS-CoV-2 NP associates with G3BP1 and G3BP2 and . NP could efficiently suppress G3BP-mediated SG formation and potentiate viral infection by overcoming G3BP1-mediated antiviral innate immunity. G3BP1 conditional knockout mice (, -Cre) exhibit significantly higher lung viral loads after SARS-CoV-2 infection than wild-type mice. Our findings contribute to the growing body of knowledge regarding the pathogenicity of NP and provide insight into new therapeutics targeting NP. In this study, by assay and live SARS-CoV-2 virus infection, we provide solid evidence that the SARS-CoV-2 NP associates with G3BP1 and G3BP2 and . NP could efficiently suppress G3BP-mediated SG formation and potentiate viral infection by overcoming antiviral innate immunity mediated by G3BP1 in A549 cell lines and G3BP1 conditional knockout mice (-cKO) mice, which provide in-depth evidence showing the mechanism underlying NP-related SARS-CoV-2 pathogenesis through G3BPs.
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http://dx.doi.org/10.1128/jvi.00412-22DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9215227PMC
June 2022

Aramid textile with near-infrared laser-induced graphene for efficient adsorption materials.

J Hazard Mater 2022 Aug 16;436:129150. Epub 2022 May 16.

Jiangsu Key Laboratory of Environmentally Friendly Polymeric Materials, School of Materials Science and Engineering, Jiangsu Collaborative Innovation Center of Photovoltaic Science and Engineering, Changzhou University, Changzhou 213164, China. Electronic address:

Porous carbon materials show great application potential in the field of adsorption. However, the preparation process of carbon adsorption materials relies on high temperature, high energy consumption, many steps, and long time. Most of them exist in the form of powder or block, and the practical application scenarios are limited and difficult to recycle. In this study, based on in-situ carbonization of polymer precursor, we directly generated laser-induced graphene (LIG) on the surface of commercial aramid textile using a low-energy near-infrared laser in air, and prospected the application prospect of the prepared aramid/graphene textile in the field of adsorption. Under a certain laser energy, the photothermal reaction promotes the breaking of the CO and CN bonds in the surface layer of the aramid fiber, and reorganizes into a graphene structure at an instantaneous high temperature, while the overall flexible structure of the textile was not destroyed. Further, adsorption materials based on the as-prepared aramid/graphene textiles were also designed, including VOC-adsorbing textile in air and dye-adsorbing textile in water. Using low-energy near-infrared laser to directly achieve LIG writing in commercial textiles under air condition will provide an efficient, environmentally friendly, and designable direction for the large-scale fabrication of textile adsorption products.
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http://dx.doi.org/10.1016/j.jhazmat.2022.129150DOI Listing
August 2022

Gold nanocluster-based ratiometric fluorescent probe for biosensing of Hg ions in living organisms.

Analyst 2022 Jun 13;147(12):2773-2778. Epub 2022 Jun 13.

China Light Industry Key Laboratory of Meat Microbial Control and Utilization, School of Food and Biological Engineering, Hefei University of Technology, Hefei,230009, China.

Gold nanoclusters (Au NCs) have become a new alternative to conventional fluorescent probes in biosensing and imaging. Herein, a gold nanocluster-based nanocomplex displaying single-excitation and dual-emission fluorescence property was fabricated by the conjugation of red-emitting glutathione-protected gold nanoclusters (Au-GSH NCs) and green-emitting fluorescein isothiocyanate (FITC) molecules. The inorganic-organic nanocomplex possesses good ratiometric fluorescence sensing ability with one emission peak showing a sensitive fluorescence response towards Hg ions and the other acting as the internal reference. The nanocomplex was demonstrated to have high stability, excellent biocompatibility, high intracellular penetrability and good biological imaging ability. It was employed as a sensitive nanosensor for rapid sensing and imaging of Hg ions in living cells and zebrafish with high contrast.
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http://dx.doi.org/10.1039/d2an00369dDOI Listing
June 2022

Acute pancreatitis in pregnancy: a 10-year, multi-center, retrospective study in Beijing.

BMC Pregnancy Childbirth 2022 May 17;22(1):414. Epub 2022 May 17.

Department of Internal Medicine, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, 251 Yaojiayuan Road, Chaoyang District, Beijing, 100026, People's Republic of China.

Objective: Acute pancreatitis in pregnancy (APIP) is a rare and serious complication during pregnancy. It has acute onset and is difficult to diagnose and treat. The aim of the present study was to describe the etiology, clinical manifestations, and maternofetal outcomes of APIP.

Methods: We retrospectively reviewed 32 pregnant women who were treated at three tertiary care hospitals in Beijing, China. The correlation between the causes of APIP, severity, laboratory indices, and outcomes was analyzed.

Results: The most common causes of APIP were hypertriglyceridemia (56.2%,18/32) and gallstones (28.1%, 9/32). Hypertriglyceridemia-induced APIP was associated with a higher rate of severe acute pancreatitis (P = 0.025). Serum level of triglycerides showed a positive correlation with the severity of APIP (P = 0.039). The most frequent presentation of APIP was abdominal pain (93.7%, 30/32). There were no maternal or fetal deaths in our study. Apgar scores at 1 min, 5 min, and 10 min of the premature neonates was correlated with the severity of APIP of the mother (P = 0.022; 0.002; 0.002).

Conclusion: High level of triglycerides may serve as a useful marker of the severity of APIP. The severity of APIP was associated with higher risk of neonate asphyxia. Appropriate timing of termination of pregnancy is a key imperative for APIP patients.
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http://dx.doi.org/10.1186/s12884-022-04742-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9115992PMC
May 2022

[Study on the accuracy of automatic segmentation of knee CT images based on deep learning].

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2022 May;36(5):534-539

Senior Department of Orthopedics, the Fourth Medical Center of Chinese PLA General Hospital, Beijing, 100048, P. R. China.

Objective: To develop a neural network architecture based on deep learning to assist knee CT images automatic segmentation, and validate its accuracy.

Methods: A knee CT scans database was established, and the bony structure was manually annotated. A deep learning neural network architecture was developed independently, and the labeled database was used to train and test the neural network. Metrics of Dice coefficient, average surface distance (ASD), and Hausdorff distance (HD) were calculated to evaluate the accuracy of the neural network. The time of automatic segmentation and manual segmentation was compared. Five orthopedic experts were invited to score the automatic and manual segmentation results using Likert scale and the scores of the two methods were compared.

Results: The automatic segmentation achieved a high accuracy. The Dice coefficient, ASD, and HD of the femur were 0.953±0.037, (0.076±0.048) mm, and (3.101±0.726) mm, respectively; and those of the tibia were 0.950±0.092, (0.083±0.101) mm, and (2.984±0.740) mm, respectively. The time of automatic segmentation was significantly shorter than that of manual segmentation [(2.46±0.45) minutes (64.73±17.07) minutes; =36.474, <0.001). The clinical scores of the femur were 4.3±0.3 in the automatic segmentation group and 4.4±0.2 in the manual segmentation group, and the scores of the tibia were 4.5±0.2 and 4.5±0.3, respectively. There was no significant difference between the two groups ( =1.753, =0.085; =0.318, =0.752).

Conclusion: The automatic segmentation of knee CT images based on deep learning has high accuracy and can achieve rapid segmentation and three-dimensional reconstruction. This method will promote the development of new technology-assisted techniques in total knee arthroplasty.
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http://dx.doi.org/10.7507/1002-1892.202201072DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9108645PMC
May 2022

White matter regeneration induced by aligned fibrin nanofiber hydrogel contributes to motor functional recovery in canine T12 spinal cord injury.

Regen Biomater 2022 29;9:rbab069. Epub 2021 Nov 29.

State Key Laboratory of New Ceramics and Fine Processing, Key Laboratory of Advanced Materials, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China.

A hierarchically aligned fibrin hydrogel (AFG) that possesses soft stiffness and aligned nanofiber structure has been successfully proven to facilitate neuroregeneration and . However, its potential in promoting nerve regeneration in large animal models that is critical for clinical translation has not been sufficiently specified. Here, the effects of AFG on directing neuroregeneration in canine hemisected T12 spinal cord injuries were explored. Histologically obvious white matter regeneration consisting of a large area of consecutive, compact and aligned nerve fibers is induced by AFG, leading to a significant motor functional restoration. The canines with AFG implantation start to stand well with their defective legs from 3 to 4 weeks postoperatively and even effortlessly climb the steps from 7 to 8 weeks. Moreover, high-resolution multi-shot diffusion tensor imaging illustrates the spatiotemporal dynamics of nerve regeneration rapidly crossing the lesion within 4 weeks in the AFG group. Our findings indicate that AFG could be a potential therapeutic vehicle for spinal cord injury by inducing rapid white matter regeneration and restoring locomotion, pointing out its promising prospect in clinic practice.
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http://dx.doi.org/10.1093/rb/rbab069DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9089163PMC
November 2021

Delivery prediction by quantitative analysis of four steroid metabolites with liquid chromatography tandem mass spectrometry in asymptomatic pregnant women.

Ann Med 2022 12;54(1):1150-1159

Department of Laboratory Medicine, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing Maternal and Child Health Care Hospital, Beijing, China.

Background: Prediction of delivery is important for assessing due dates, providing adequate prenatal care, and suggesting appropriate interventions in preterm and post-term pregnancies. Recent metabolomic findings suggested that the temporal abundance information of metabolome can be used to predict delivery timing with high accuracy in a cohort of healthy women. However, a targeted and quantitative assay is required to further validate the clinical performance and utility of this group of metabolomic candidates in delivery prediction with a larger and independent cohort.

Method: LC-MS/MS quantitative assays were applied to determine the plasma concentrations of four steroid metabolites, including oestriol-16-glucuronide (E3-16-Gluc), 17-alpha-hydroxyprogesterone (17-OHP), tetrahydrodeoxycorticosterone (THDOC), and androstane-3,17-diol (A-3,17-Diol) in asymptomatic women of singleton pregnancies (≥30 gestational weeks). Subsequent statistical analysis was conducted to assess the performance of the above candidates in delivery prediction.

Result: Using LC-MS/MS, four steroids were separated and quantified in 5.5 min. The coefficients of variation (CVs) of the four analytes at the lower limit of quantification ranged from 7.9% to 14.6%, with the values greater than 0.990 in the calibration curves. Of the 585 recruited pregnant women who ended up with spontaneous delivery, 17.1% and 82.9% of the subjects delivered within and after 7 days since plasma collection, respectively. In the receiver operator curve analysis, the gestational age-adjusted area under the curve of the combined measurements of E3-16-Gluc and 17-OHP was 0.69 (95% CI: 0.60-0.76), with the sensitivity of 87.0% (95% CI: 78.8%-92.9%) and specificity of 60.2% (95% CI: 55.7%-64.6%). Moreover, the positive and the negative predictive values were 28.3%-34.0% and 93.1%-97.4% respectively for this combined panel.

Conclusion: We performed analytical and clinical validation of a quantitation LC-MS/MS panel for the four steroids in the plasma of pregnant women. The steroid metabolites panel of E3-16-Gluc and 17-OHP was potentially useful for predicting delivery within one week in asymptomatic women of singleton pregnancies. Key messagesA quantitative LC-MS/MS assay for determining the plasma levels of 17-OHP, THDOC, A-3,17-Diol and E3-16-Gluc was developed and validated, in order to evaluate their predictive performance in asymptomatic delivery of singleton pregnancy. The levels of E3-16-Gluc and 17-OHP were found to be significantly elevated at the time of sampling in women that delivered within one week and their combinational testing may be potentially useful in delivery prediction.
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http://dx.doi.org/10.1080/07853890.2022.2067895DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9045778PMC
December 2022

PINK1/Parkin-mediated mitophagy as a protective mechanism against AFB-induced liver injury in mice.

Food Chem Toxicol 2022 Jun 18;164:113043. Epub 2022 Apr 18.

Key Laboratory of the Provincial Education, Department of Heilongjiang for Common Animal Disease Prevention and Treatment, College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China. Electronic address:

Aflatoxin B (AFB) can cause oxidative stress leading to mitochondrial damage and subsequent liver injury. Although it is well-known that damaged mitochondria are eliminated by PINK1/Parkin-mediated mitophagy, this mechanism has not yet been characterized in the context of AFB-induced liver injury. In this study, male wild-type C57BL/6N mice were divided into groups 1-4, which were then orally administered 0, 0.5, 0.75, and 1 mg/kg body weight AFB for 28 d, respectively. Our results demonstrated that oxidative stress, NLRP3-inflammasome activation, and mitochondrial damage were dose-dependently augmented in AFB-induced liver injury. Additionally, PINK1/Parkin-mediated mitophagy peaked in the groups that had received a mid-dose of AFB (0.75 mg/kg), which was attenuated slightly in high-dose groups. Afterward, we further characterized AFB-induced liver injury by comparing wild-type C57BL/6N mice with Parkin knockout (Parkin) mice. We found that the restricted mitophagy in Parkin mice was associated with increased oxidative stress, NLRP3-inflammasome activation, mitochondrial damage, and liver injury. Taken together, these results indicate that PINK1/Parkin-mediated mitophagy plays an important role in attenuating AFB-induced liver injury in mice.
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http://dx.doi.org/10.1016/j.fct.2022.113043DOI Listing
June 2022

What Affects Emotional Well-Being during Travel? Identifying the Factors by Maximal Information Coefficient.

Int J Environ Res Public Health 2022 04 4;19(7). Epub 2022 Apr 4.

Jiangsu Key Laboratory of Urban ITS, School of Transportation, Southeast University, Nanjing 211189, China.

Traveler emotional well-being as a specific domain of subjective well-being has attracted attention across the field of transportation. Studies on identifying factors of travel-related emotional well-being can help policy makers to formulate concrete strategies to improve travelers' experiences and public health. This research used the Maximal Information Coefficient (MIC) to select important factors which have much influence on emotional well-being during travel. American Time Use Survey data collected in 2010, 2012, and 2013 were used in this study and 10 factors have been selected to illustrate the relationship with emotional well-being, including rest, weekly earnings, activity time for well-being, health, self-evaluation of activities, pain medication taken yesterday, travel purpose, travel duration, weekly working hours and age based on MIC values in Descending sort. Among these 10 selected features, 2 factors, travel purpose and travel duration, are related to travel contexts; the other factors are related to personal and social characteristics. It is found that an individual's physical condition and self-evaluation of activities have much influence on travel-related emotional well-being, while traveling mode and interaction during travel have a relatively small impact on emotional well-being compared to other identified factors. This finding is different from previous research findings. The paper presents traffic strategies related to improving emotional well-being of travelers while traveling based on the findings from this research.
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http://dx.doi.org/10.3390/ijerph19074326DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8998290PMC
April 2022

Comprehensive analysis of the differential cellular and EBV miRNA expression profiles in mature T and NK cell lymphomas.

Pathol Res Pract 2022 May 20;233:153846. Epub 2022 Mar 20.

Department of Pathology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, China. Electronic address:

Epstein-Barr virus (EBV) has been detected in several subtypes of Mature T and NK cell lymphomas. However, the impact of EBV status on the survival and the mechanism is still unknown. Numerous recent investigations have demonstrated that the EBV-encoded microRNAs (EBV-miRNAs) were associated with the development and prognosis of lymphoma. We used a miRNA microarray to identify the differentially expressed miRNAs in 175 Mature T and NK cell lymphomas including 5 subtypes. The result demonstrated that all the samples could be divided into three groups according to the relative expression of EBV-miRNAs, and the prognosis of the three groups was significantly different (P < 0.05). We also found that there were 72 differential cellular miRNAs with 11 up-regulated and 61 down-regulated miRNAs in the group with poorer prognosis compared with the group with better prognosis. GO and KEGG pathway analysis indicated that genes targeted by the upregulated miRNAs were significantly enriched in multiple tumor-related pathways including MAPK signaling pathway, Wnt signaling pathway, Focal adhesion and ErbB signaling pathway, while genes targeted by the downregulated miRNAs were significantly enriched in Axon guidance and VEGF signaling pathway. The current findings demonstrated that the higher expression of EBV-miRNA was related to shorter overall survival, and the signaling pathways enriched in the group with EBV infection could provide theoretical basis for the potential target therapy of the Mature T and NK cell lymphoma.
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http://dx.doi.org/10.1016/j.prp.2022.153846DOI Listing
May 2022

A two-stage deep learning architecture for radiographic staging of periodontal bone loss.

BMC Oral Health 2022 04 1;22(1):106. Epub 2022 Apr 1.

Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, Zhejiang Provincial Clinical Research Center for Oral Diseases, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Cancer Center of Zhejiang University, Hangzhou, 310006, China.

Background: Radiographic periodontal bone loss is one of the most important basis for periodontitis staging, with problems such as limited accuracy, inconsistency, and low efficiency in imaging diagnosis. Deep learning network may be a solution to improve the accuracy and efficiency of periodontitis imaging staging diagnosis. This study aims to establish a comprehensive and accurate radiological staging model of periodontal alveolar bone loss based on panoramic images.

Methods: A total of 640 panoramic images were included, and 3 experienced periodontal physicians marked the key points needed to calculate the degree of periodontal alveolar bone loss and the specific location and shape of the alveolar bone loss. A two-stage deep learning architecture based on UNet and YOLO-v4 was proposed to localize the tooth and key points, so that the percentage of periodontal alveolar bone loss was accurately calculated and periodontitis was staged. The ability of the model to recognize these features was evaluated and compared with that of general dental practitioners.

Results: The overall classification accuracy of the model was 0.77, and the performance of the model varied for different tooth positions and categories; model classification was generally more accurate than that of general practitioners.

Conclusions: It is feasible to establish deep learning model for assessment and staging radiographic periodontal alveolar bone loss using two-stage architecture based on UNet and YOLO-v4.
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http://dx.doi.org/10.1186/s12903-022-02119-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8973652PMC
April 2022

Automatic segmentation and detection of ectopic eruption of first permanent molars on panoramic radiographs based on nnU-Net.

Int J Paediatr Dent 2022 Mar 21. Epub 2022 Mar 21.

Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, Zhejiang Provincial Clinical Research Center for Oral Diseases, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Cancer Center of Zhejiang University, Hangzhou, China.

Aim: The purpose of this research was to present an artificial intelligence (AI) model, which can automatically segment and detect ectopic eruption of first permanent molars (EMMs) in early mixed dentition on panoramic radiographs using the no-new-Net (nnU-Net) model.

Design: A total of 438 EMMs obtained from 285 panoramic radiographs were included in this study. An AI model based on nnU-Net was trained to segment and detect EMMs. The performance of the model was evaluated by the intersection over union (IoU), precision, F1-score, accuracy and FROC. Furthermore, the detecting performance of nnU-Net was compared with that of three dentists with different years of experience using the McNemar chi-squared test. The reliability of different dentists was evaluated by intraclass correlation coefficients (ICCs).

Results: The nnU-Net yielded an IoU of 0.834, a precision of 0.845, an F1-score of 0.902 and an accuracy of 0.990, whereas the dentists yielded a mean IoU of 0.530, a mean precision of 0.539, a mean F1-score of 0.699 and a mean accuracy of 0.811. The ICC of different dentists was 0.776. The statistical analysis of the McNemar chi-squared test showed that the nnU-Net results were statistically significant and superior to those of dentists (p < .05).

Conclusion: This study validated an AI model based on nnU-Net for automatically segmenting and detecting EMMs more consistently and accurately on panoramic radiography.
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http://dx.doi.org/10.1111/ipd.12964DOI Listing
March 2022

PINK1/Parkin-mediated mitophagy is activated to protect against AFB-induced kidney damage in mice.

Chem Biol Interact 2022 May 15;358:109884. Epub 2022 Mar 15.

Key Laboratory of the Provincial Education, Department of Heilongjiang for Common Animal Disease Prevention and Treatment, College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China. Electronic address:

Aflatoxin B (AFB) is a toxic food pollutant that has extensive deleterious impacts on the kidney. Oxidative stress represents the primary mechanism of AFB nephrotoxicity and can also cause mitochondrial damage. Damaged mitochondria can trigger apoptosis leading to kidney injury. PINK1/Parkin-mediated mitophagy can alleviate mitochondrial injury to maintain cellular homeostasis, however, its role in AFB-induced kidney damage is unknown. To investigate the effect of PINK1/Parkin-mediated mitophagy on kidney impairment triggered by AFB, 40 male wild-type (WT) C57BL/6N mice were first assigned to 4 groups and orally exposed to AFB at 0, 0.5, 0.75, and 1 mg/kg body weight (BW) for 28 days. The results revealed that AFB induced kidney damage, oxidative stress, mitochondrial damage, apoptosis and activated PINK1/Parkin-mediated mitophagy with a dose-dependent effect. Then, 20 male WT C57BL/6N mice and 20 male Parkin knockout (Parkin) C57BL/6N mice were assigned to 4 groups and orally exposed to AFB at 0, 1, 0, and 1 mg/kg BW for 28 days. The results revealed that Parkin suppressed mitophagy and exacerbated kidney damage, oxidative stress, mitochondrial damage, and apoptosis under AFB exposure. The aforementioned evidences demonstrate that PINK1/Parkin-mediated mitophagy is activated by AFB and protects against kidney damage in mice.
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http://dx.doi.org/10.1016/j.cbi.2022.109884DOI Listing
May 2022
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