Publications by authors named "Zhenfa Li"

4 Publications

  • Page 1 of 1

circALPL Sponges miR-127 to Promote Gastric Cancer Progression by Enhancing MTDH Expression.

J Cancer 2021 11;12(16):4924-4932. Epub 2021 Jun 11.

Affiliated Hengyang Hospital, Southern Medical University (Hengyang Central Hospital), 12# Yancheng road, Hengyang 421001, Hunan province, China.

CircRNA plays an important role in cancer progression. However, the potential mechanism of circRNA in gastric cancer remains unknown. In this study, we aimed to investigate the specific mechanism of circALPL in gastric cancer. : Using a high-throughput microarray, we found that circALPL was upregulated in gastric cancer cell lines. RT-qPCR was used to measure the circALPL expression level in gastric cell lines and tissue. Transwell, CCK-8, and metastasis assays were performed to learn the function after circALPL was inhibited. : circALPL downregulation suppresses the invasion and proliferation ability of gastric cancer cells. Additionally, the underlying pathway of circALPL was studied using luciferase reporter assays and RNA immunoprecipitation assays. The results showed that circALPL promotes gastric cancer progression by sponging miR-127, thus upregulating MTDH. The circALPL-miR-127-MTDH pathway plays a vital role in gastric cancer proliferation and metastasis. circALPL might be a new therapeutic target in gastric cancer.
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http://dx.doi.org/10.7150/jca.49942DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8247368PMC
June 2021

Additive-Free Copper(I)-Mediated Synthesis of 5- or 6-Brominated 2-Aryl-1-Indole-3-Carboxylates from α,α-Dibromo β-Iminoesters.

J Org Chem 2021 01 4;86(2):1964-1971. Epub 2021 Jan 4.

College of Pharmaceutical Engineering of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, P. R. China.

Additive-free copper(I)-bromide-mediated radical cyclization reactions of α,α-dibromo β-iminoesters were investigated, enabling the synthesis of a series of 5- or 6-brominated 2-aryl-1-indole-3-carboxylates in moderate to good yields. The mechanistic study showed that (i) the bromine atom originated from the substrates and (ii) the bromination might be related to a 3-bromo-3 indole intermediate via an electrophilic bromine atom transfer. Furthermore, the practicality of this method was demonstrated by gram-scale synthesis and the potential for product derivatization toward other valuable multisubstituted indoles.
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http://dx.doi.org/10.1021/acs.joc.0c02497DOI Listing
January 2021

Ten New Dammarane-Type Saponins with Hypolipidemia Activity from a Functional Herbal Tea-.

Molecules 2020 Aug 15;25(16). Epub 2020 Aug 15.

College of Pharmaceutical Engineering of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China.

(thumb.) Makino is a functional herbal tea commonly used in Asian countries and regions to reduce blood lipid levels. saponin is the main component, but there are still a large number of components with lipid-lowering activity that have not been found. In this study, 10 novel dammarane-type saponins, (-) and a known one () were isolated from . Ten new compounds were identified and named as yunnangypenosides A-J (-), and another known one () was also obtained. Their chemical structures were determined by MS, NMR spectroscopic analyses. Moreover, the cytotoxicities on human HepG-2 hepatocellular carcinoma cells of these isolates were evaluated, and the results showed that compounds - had no obvious cytotoxicity. Finally, all these compounds were evaluated for their lipid-lowering effect by means of the oil red O staining method. Ten compounds could significantly reduce lipid levels except of , especially exhibite the strongest hypolipidemia activity.
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http://dx.doi.org/10.3390/molecules25163737DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7464191PMC
August 2020

Medium- and long-term effects of endovascular treatments for severely stenotic basilar arteries supported by multimodal imaging.

BMC Neurol 2020 Jul 31;20(1):289. Epub 2020 Jul 31.

Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou, 510282, China.

Background: To evaluate the medium-and long-term effect of intravascular interventional therapy for symptomatic severe basilar artery stenosis supported by multimodal imaging.

Method: After strict screening of 67 patients with symptomatic severe basilar artery stenosis (70-99%) with atherosclerotic stenosis, 67 patients with symptomatic recurrence after intensive drug treatment were treated with intravascular balloon dilatation and Enterprise stent implantation. Any stroke or death within 30 days after operation and any stroke and restenosis during medium-and long-term follow-up were recorded.

Results: ①The mean age of 67 patients (67lesions) was 57 ± 8 years old, and the technical success rate was 100%; ②Preoperative angiography showed that the collateral circulation was poor, and TICI was 1-2a while postoperative angiography showed that TICI was significantly improved to 2b-3; ③The average preoperative stenosis rate was 82 ± 9%, and the postoperative stenosis rate was reduced to 17 ± 10%; ④Before surgery, abnormal perfusion was found in the posterior circulation CTP; After the postoperative re-examination, the posterior circulation of CTP perfusion was significantly improved; ⑤Postoperative symptoms and neurological conditions improved significantly; ⑥Complications of perforating branch event occurred in 1 case after operation, and symptoms were relieved after more than 1 month of medication treatment, and mild neurological dysfunction remained. 1 case developed subacute thrombosis in the stent, which improved after active intra-arterial thrombolysis, and there was no residual neurological dysfunction; and 1 case of micro-guide wire being trapped by the distal vasospasm. ⑦67 patients were followed up by telephone, WeChat or imaging for 36-66 months.

Conclusions: In summary intravascular balloon dilation + Enterprise stent implantation is safe and effective for the treatment of symptomatic severe atherosclerotic stenosis of the basilar artery, with high technical success rate, low perioperative complications, and good mid-term and long-term effects.
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http://dx.doi.org/10.1186/s12883-020-01863-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7393701PMC
July 2020
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