Publications by authors named "Zhendong Liu"

113 Publications

An Improved Method for Stable Feature Points Selection in Structure-from-Motion Considering Image Semantic and Structural Characteristics.

Sensors (Basel) 2021 Apr 1;21(7). Epub 2021 Apr 1.

College of Geodesy and Geomatics, Shandong University of Science and Technology, Qingdao 266590, China.

Feature matching plays a crucial role in the process of 3D reconstruction based on the structure from motion (SfM) technique. For a large collection of oblique images, feature matching is one of the most time-consuming steps, and the matching result directly affects the accuracy of subsequent tasks. Therefore, how to extract the reasonable feature points robustly and efficiently to improve the matching speed and quality has received extensive attention from scholars worldwide. Most studies perform quantitative feature point selection based on image Difference-of-Gaussian (DoG) pyramids in practice. However, the stability and spatial distribution of feature points are not considered enough, resulting in selected feature points that may not adequately reflect the scene structures and cannot guarantee the matching rate and the aerial triangulation accuracy. To address these issues, an improved method for stable feature point selection in SfM considering image semantic and structural characteristics is proposed. First, the visible-band difference vegetation index is used to identify the vegetation areas from oblique images, and the line feature in the image is extracted by the optimized line segment detector algorithm. Second, the feature point two-tuple classification model is established, in which the vegetation area recognition result is used as the semantic constraint, the line feature extraction result is used as the structural constraint, and the feature points are divided into three types. Finally, a progressive selection algorithm for feature points is proposed, in which feature points in the DoG pyramid are selected by classes and levels until the number of feature points is satisfied. Oblique images of a 40-km area in Dongying city, China, were used for validation. The experimental results show that compared to the state-of-the-art method, the method proposed in this paper not only effectively reduces the number of feature points but also better reflects the scene structure. At the same time, the average reprojection error of the aerial triangulation decrease by 20%, the feature point matching rate increase by 3%, the selected feature points are more stable and reasonable.
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http://dx.doi.org/10.3390/s21072416DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8036694PMC
April 2021

Facile synthesis of Fe-baicalein nanoparticles for photothermal/chemodynamic therapy with accelerated Fe/Fe conversion.

J Mater Chem B 2021 Apr 12;9(15):3295-3299. Epub 2021 Apr 12.

State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Science, Changchun 130022, P. R. China.

Fe-baicalein-polyethylene glycol (Fe-BaP) nanoparticles were synthesized by a room temperature wet chemical method via coordination between Fe and baicalein. Fe-BaP possessed high photothermal conversion efficiency (η = 45.6%) and excellent antitumor efficacy was achieved with the synergistic photothermal/chemodynamic tumor therapy.
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http://dx.doi.org/10.1039/d1tb00200gDOI Listing
April 2021

Novel LncRNA OXCT1-AS1 indicates poor prognosis and contributes to tumorigenesis by regulating miR-195/CDC25A axis in glioblastoma.

J Exp Clin Cancer Res 2021 Apr 8;40(1):123. Epub 2021 Apr 8.

Department of Neurosurgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Jiefang Road 88th, Hangzhou, 310016, Zhejiang Province, People's Republic of China.

Background: Long noncoding RNAs (lncRNAs) contribute to multiple biological processes in human glioblastoma (GBM). However, identifying a specific lncRNA target remains a challenge. In this study, bioinformatics methods and competing endogenous RNA (ceRNA) network regulatory rules were used to identify GBM-related lncRNAs and revealed that OXCT1 antisense RNA 1 (OXCT1-AS1) is a potential therapeutic target for the treatment of glioma.

Methods: Based on the Gene Expression Omnibus (GEO) dataset, we identified differential lncRNAs, microRNAs and mRNAs and constructed an lncRNA-associated ceRNA network. The novel lncRNA OXCT1-AS1 was proposed to function as a ceRNA, and its potential target miRNAs were predicted through the database LncBase Predicted v.2. The expression patterns of OXCT1-AS1 in glioma and normal tissue samples were measured. The effect of OXCT1-AS1 on glioma cells was checked using the Cell Counting Kit 8 assay, cell colony formation assay, Transwell assay and flow cytometry in vitro. The dual-luciferase activity assay was performed to investigate the potential mechanism of the ceRNA network. Finally, orthotopic mouse models of glioma were created to evaluate the influence of OXCT1-AS1 on tumour growth in vivo.

Results: In this study, it was found that the expression of lncRNA OXCT1-AS1 was upregulated in both The Cancer Genome Atlas (TCGA) GBM patients and GBM tissue samples, and high expression of OXCT1-AS1 predicted a poor prognosis. Suppressing OXCT1-AS1 expression significantly decreased GBM cell proliferation and inhibited cell migration and invasion. We further investigated the potential mechanism and found that OXCT1-AS1 may act as a ceRNA of miR-195 to enhance CDC25A expression and promote glioma cell progression. Finally, knocking down OXCT1-AS1 notably attenuated the severity of glioma in vivo.

Conclusion: OXCT1-AS1 inhibits glioma progression by regulating the miR-195-5p/CDC25A axis and is a specific tumour marker and a novel potential therapeutic target for glioma treatment.
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http://dx.doi.org/10.1186/s13046-021-01928-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8028723PMC
April 2021

Displacement Identification by Computer Vision for Condition Monitoring of Rail Vehicle Bearings.

Sensors (Basel) 2021 Mar 17;21(6). Epub 2021 Mar 17.

State Key Laboratory of Traction Power, Southwest Jiaotong University, Chengdu 610036, China.

Bearings of rail vehicles bear various dynamic forces. Any fault of the bearing seriously threatens running safety. For fault diagnosis, vibration and temperature measured from the bogie and acoustic signals measured from trackside are often used. However, installing additional sensing devices on the bogie increases manufacturing cost while trackside monitoring is susceptible to ambient noise. For other application, structural displacement based on computer vision is widely applied for deflection measurement and damage identification of bridges. This article proposes to monitor the health condition of the rail vehicle bearings by detecting the displacement of bolts on the end cap of the bearing box. This study is performed based on an experimental platform of bearing systems. The displacement is monitored by computer vision, which can image real-time displacement of the bolts. The health condition of bearings is reflected by the amplitude of the detected displacement by phase correlation method which is separately studied by simulation. To improve the calculation rate, the computer vision only locally focuses on three bolts rather than the whole image. The displacement amplitudes of the bearing system in the vertical direction are derived by comparing the correlations of the image's gray-level co-occurrence matrix (GLCM). For verification, the measured displacement is checked against the measurement from laser displacement sensors, which shows that the displacement accuracy is 0.05 mm while improving calculation rate by 68%. This study also found that the displacement of the bearing system increases with the increase in rotational speed while decreasing with static load.
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http://dx.doi.org/10.3390/s21062100DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8002472PMC
March 2021

rBPDL: Predicting RNA-binding proteins using deep learning.

IEEE J Biomed Health Inform 2021 Mar 29;PP. Epub 2021 Mar 29.

RNA-binding protein (RBP) is a powerful and wide-ranging regulator that plays an important role in cell development, differentiation, metabolism, health and disease. The prediction of RBPs provides valuable guidance for biologists; although the wet test RBP has made good progress, it is time-consuming and not flexible. Therefore, we developed a network model, rBPDL, by combining a convolutional neural network and long short-term memory for multilabel classification of RBPs. Moreover, to achieve better prediction results, we used a voting algorithm for ensemble learning of the model. We compared rBPDL with state-of-the-art methods and found that rBPDL significantly improved identification performance for the RBP68 dataset, with a macro-Area Under Curve (AUC), micro-AUC, and weighted AUC of 0.936, 0.962, and 0.946, respectively. Furthermore, we analyzed the performance of rBPDL on a single RBP and found, through AUC statistical analysis of the RBP domain, that the RBP identification performance in the same domain was similar. In addition, we analyzed the performance preferences and physicochemical properties of the binding protein amino acids and explored the characteristics that affect the binding by using the RBP86 dataset. The code and datasets can be found at the link: https://github.com/nmt315320/rBPDL.git.
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http://dx.doi.org/10.1109/JBHI.2021.3069259DOI Listing
March 2021

The increasing expression of GPX7 related to the malignant clinical features leading to poor prognosis of glioma patients.

Chin Neurosurg J 2021 Mar 10;7(1):21. Epub 2021 Mar 10.

Department of Neurosurgery, First Affiliated Hospital of Harbin Medical University, No. 23 Youzheng Street, Nangang District, Harbin, 150001, Heilongjiang Province, China.

Background: Glioma is the most common malignant brain tumor in adults. The standard treatment scheme of glioma is surgical resection combined alternative radio- and chemotherapy. However, the outcome of glioma patients was unsatisfied. Here, we aimed to explore the molecular and biological function characteristics of GPX7 in glioma.

Methods: The multidimensional data of glioma samples were downloaded from Chinese Glioma Genome Atlas (CGGA). RT-qPCR method was used to identify the expression status of GPX7. Kaplan-Meier curves and Cox regression analysis were used to explore the prognostic value of GPX7. Gene Set Enrichment Analysis (GSEA) was applied to investigate the GPX7-related functions in glioma.

Results: The results indicated that the expression of GPX7 in glioma was higher compared to that in normal brain tissue. Univariate and multivariate Cox regression analyses confirmed that the expression value of GPX7 was an independent prognostic factor in glioma. The GSEA analysis showed that GPX7 was significantly enriched in the cell cycle pathway, ECM pathway, focal adhesion pathway, and toll-like receptor pathway.

Conclusions: The GPX7 was recommended as an independent risk factor for patients diagnosed with glioma for the first time and GPX7 could be potentially used as the therapy target in future. Furthermore, we attempted to explore a potential biomarker for improving the diagnosis and prognosis of patients with glioma.
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http://dx.doi.org/10.1186/s41016-021-00235-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7945363PMC
March 2021

Covalent Organic Framework-Titanium Oxide Nanocomposite for Enhanced Sonodynamic Therapy.

Bioconjug Chem 2021 04 12;32(4):661-666. Epub 2021 Mar 12.

State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Science, Changchun 130022, P. R. China.

Sonodynamic therapy (SDT) has attracted wide attention for its high tissue-penetration depth capacity. However, developing new kinds of sonosensitizers that are capable of generating large amounts of reactive oxygen species (ROS) still remains a challenge. Herein, covalent organic framework-titanium oxide nanoparticles (COF-TiO NPs) were successfully synthesized by using COF as a template. Under ultrasound (US) irradiation, large quantities of ROS can be generated, and compared with pure TiO NPs, the SDT performance of COF-TiO nanoparticles was significantly improved due to the narrower band gap. Both in vitro and in vivo experiments demonstrated the great tumor inhibitory effect via COF-TiO-mediated SDT. This work broadens the biomedical applications of COF-based composites.
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http://dx.doi.org/10.1021/acs.bioconjchem.1c00039DOI Listing
April 2021

Interleukin-1 receptor-associated kinase 4 as a potential biomarker: Overexpression predicts poor prognosis in patients with glioma.

Oncol Lett 2021 Apr 3;21(4):254. Epub 2021 Feb 3.

Department of Orthopaedics, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, School of Clinical Medicine, Henan University, Zhengzhou, Henan 450001, P.R. China.

The undetectable onset of glioma and the difficulty of surgery lead to a poor prognosis. Appropriate biomarkers for diagnosis and treatment need to be identified. Interleukin-1 receptor-associated kinase 4 (IRAK4) is involved in the initiation and progression of cancer. However, up until now, no report has revealed the relationship between IRAK4 and glioma. The present study aimed to examine the expression of IRAK4 in glioma, and to determine if there was a relationship between IRAK4 expression and clinical outcomes or survival prognosis. Thousands of glioma tissue samples and corresponding clinical information were obtained from various databases. Then a series of bioinformatics methods were used to reveal the role of IRAK4 in glioma. Finally, reverse transcription-quantitative PCR technology was used to verify the bioinformatics results. The study found that the expression of IRAK4 was significantly increased in glioma compared with the control brain tissue samples, and IRAK4, as an independent prognostic factor, shortened the overall survival time of patients with glioma. Gene Set Enrichment Analysis showed that IRAK4 promoted the activation of cell signalling pathways, such as NOD-like and Toll-like receptor signalling pathways. Co-expression analysis showed that the expression of IRAK4 was correlated with CMTM6, MOB1A and other genes. The present study demonstrated the role of IRAK4 as an oncogene in the pathological process of glioma for the first time, and highlights the potential of IRAK4 as a biomarker for prognostic evaluation and treatment of glioma.
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http://dx.doi.org/10.3892/ol.2021.12516DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7882878PMC
April 2021

High-Density Genetic Linkage Map Construction and White Rot Resistance Quantitative Trait Loci Mapping for Genus Based on Restriction Site-Associated DNA Sequencing.

Phytopathology 2021 Apr 26;111(4):659-670. Epub 2021 Feb 26.

College of Horticulture, Shenyang Agricultural University, Shenyang 110866, People's Republic of China.

Grape white rot () is a major fungal disease affecting grape yield and quality. Quantitative trait locus (QTL) analysis is an important method for studying important horticultural traits of grapevine. This study was conducted to construct a high-density map and conduct QTL mapping for grapevine white rot resistance. A mapping population with 177 genotypes was developed from interspecific hybridization of a white rot-resistant cultivar ( × 'Zhuosexiang') and white rot-susceptible cultivar ( 'Victoria'). Single-nucleotide polymorphism (SNP) markers were developed by restriction site-associated DNA sequencing. The female, male, and integrated maps contained 2,501, 4,110, and 6,249 SNP markers with average genetic distances of adjacent markers of 1.25, 0.77, and 0.50 cM, respectively. QTL mapping was conducted based on white rot resistance identification of 177 individuals in July and August of 2017 and 2018. Notably, one stable QTL related to white rot resistance was detected and located on linkage group LG14. The phenotypic variance ranged from 12.93 to 13.43%. An SNP marker (chr14_3929380), which cosegregated with white rot resistance, was discovered and shows potential for use in marker-assisted selection to generate new grapevine cultivars with resistance to white rot.
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http://dx.doi.org/10.1094/PHYTO-12-19-0480-RDOI Listing
April 2021

Abnormal expression of promotes the malignant behavior of glioma cells and leads to poor prognosis of glioma patients.

PeerJ 2021 8;9:e10820. Epub 2021 Feb 8.

Department of Surgery of Spine and Spinal Cord, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, School of Clinical Medicine, Henan University, Zhengzhou, Henan, China.

Background: Homeobox D11 () plays an important role in a variety of cancers, but its precise role in gliomas remains unclear. This study aimed to explore the relationship between and gliomas by combining bioinformatics methods with basic experimental validation.

Materials And Methods: Obtain gene expression information and clinical information of glioma and non-tumor brain tissue samples from multiple public databases such as TCGA (666 glioma samples), CGGA (749 glioma samples), GEPIA(163 glioblastoma samples and 207 normal control samples), GEO (GSE4290 and GSE15824). Nine cases of glioma tissue and five cases of normal control brain tissue were collected from the clinical department of Henan Provincial People's Hospital for further verification. A series of bioinformatic analysis methods were used to confirm the relationship between expression and overall survival and clinical molecular characteristics of patients with glioma. RT-qPCR was used to verify the change of expression level of in glioma cells and tissues. MTT assay, colony formation assay, wound-healing assay, immunofluorescence staining, flow cytometry and western blotting were used to detect the effect of on the biological behavior of glioma cell line U251.

Results: The high expression of was significantly related to age, World Health Organization (WHO) grade, chemotherapy status, histological type, and even 1p19q codeletion data and isocitrate dehydrogenase (IDH) mutation. , as an independent risk factor, reduces the overall survival of glioma patients and has diagnostic value for the prognosis of glioma. Gene Set Enrichment Analysis (GSEA) showed that was significantly enriched in cell signaling pathway such as cell cycle, DNA replication and so on. Finally, we confirmed that the knockout of can inhibit the proliferation and invasion of U251 glioma cells, and change the biological behavior of tumor cells by preventing the progression of cell cycle.

Conclusions: may be used as a candidate biomarker for the clinical application of targeted drug and prognostic assessment treatment of glioma. In addition, This study will help to explore the pathological mechanism of glioma.
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http://dx.doi.org/10.7717/peerj.10820DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7877241PMC
February 2021

Total flavonoids of Radix Tetrastigma suppress inflammation-related hepatocellular carcinoma cell metastasis.

Mol Genet Genomics 2021 Feb 12. Epub 2021 Feb 12.

Department of Oncology, Xijing Hospital, Air Force Military Medical University, 15 Changle West Road, Xian, 710032, Shaanxi, China.

This study aimed to investigate the effects of the total flavonoids of Radix Tetrastigma (RTF) on inflammation-related hepatocellular carcinoma (HCC) development. Extracted RTF was diluted to different concentrations for subsequent experiments. HCC cells were cotreated with lipopolysaccharide (LPS) and RTF to investigate the effects of RTF on LPS-stimulated HCC cells. A CCK-8 kit was used to measure cell proliferation. Apoptosis was detected with a flow cytometer. Cell migration and invasion were quantified by wound healing and Transwell assays, respectively. The expression of TLR4 and COX-2 and activation of the NF-κB pathway were determined by Western blotting. Treatment with LPS significantly enhanced cell proliferation and decreased the apoptosis rate, while cell migration and invasion were notably upregulated. RTF suppressed the proliferation and invasion induced by LPS stimulation and promoted HCC cell apoptosis. The protein levels of Bax and cleaved caspase-3 were decreased and that of Bcl-2 was increased by LPS in HCC cells, which could be rescued by RTF. RTF significantly inhibited the LPS-induced expression of the proinflammatory mediators IL-6 and IL-8 in HCC cells. Mechanistically, with RTF treatment, the upregulated expression of TLR4 and COX-2 induced by LPS was obviously downregulated. Furthermore, the phosphorylation of NF-κB/p65 was significantly decreased in LPS-stimulated cells after supplementation with RTF. Our study suggests that RTF exerts a significant inhibitory effect on the LPS-induced enhancement of the malignant behaviors of HCC cells via inactivation of TLR4/NF-κB signaling. RTF may be a promising chemotherapeutic agent to limit HCC development and inflammation-mediated metastasis.
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http://dx.doi.org/10.1007/s00438-020-01759-6DOI Listing
February 2021

Urchin-Shaped Metal Organic/Hydrogen-Bonded Framework Nanocomposite as a Multifunctional Nanoreactor for Catalysis-Enhanced Synergetic Therapy.

ACS Appl Mater Interfaces 2021 Feb 26;13(4):4825-4834. Epub 2021 Jan 26.

State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Science, Changchun 130022, P. R. China.

Ultrasound (US)-induced sonodynamic therapy (SDT) is an efficient and precise method against tumor, and the integration of multiple cancer therapies has been proved as a promising strategy for better therapeutic effects. Herein, for the first time, a multifunctional nanoreactor has been fabricated by integrating Fe-MIL-88B-NH, PFC-1, and glucose oxidase (GO) to form urchin-like Fe-MIL-88B-NH@PFC-1-GO (MPG) nanoparticles as Fenton's reagent, a sonosensitizer, and a tumor microenvironment (TME) modulator. In detail, MPG can generate OH for chemodynamic therapy (CDT) and deplete glutathione (GSH) to alleviate the antioxidant ability of cancer cells. Moreover, catalase (CAT)-like MPG can react with HO to generate O for relieving hypoxia in TME, enhancing GO-catalyzed glucose oxidation to produce HO and gluconic acid. Then, the regenerated HO can promote the Fenton reaction to achieve GO catalysis-enhanced CDT. Owing to its large π-electron conjugated system, MPG also serves as an ideal sonosensitizer, realizing a burst generation of O under US irradiation for efficient SDT. Therefore, the tumor treatment will be notably enhanced by MPG-based synergetic CDT/SDT/starvation therapy via a series of cascade reactions. Overall, this work develops a versatile nanoreactor with improved tumor treatment effectiveness and broadens the application prospects of porous materials in the field of biomedical research.
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http://dx.doi.org/10.1021/acsami.0c19584DOI Listing
February 2021

Synthetic and natural MOR zeolites as high-capacity adsorbents for the removal of nitrous oxide.

Chem Commun (Camb) 2021 Feb 22;57(11):1312-1315. Epub 2021 Jan 22.

Department of Chemical System Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.

NO is typically present as a trace gas in chemical processes, but its emission causes serious environmental issues. We herein demonstrate that ion-exchanged mordenite zeolites (framework code: MOR) can exhibit high capacities for NO adsorption under ambient conditions. In particular, a natural MOR zeolite gives an adsorption capacity as high as 0.34 mmol-NO per g-zeolite (1 atm, 25 °C), representing the best performing material among all zeolite-based adsorbents reported so far. The results contribute toward a comprehensive understanding of the structure-activity relationship and offer insights to establishing a zeolite-based adsorption system for enriching or removing NO.
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http://dx.doi.org/10.1039/d0cc07511fDOI Listing
February 2021

A Review on Linking the Medicinal Functions of Mushroom Prebiotics with Gut Microbiota.

Int J Med Mushrooms 2020 ;22(10):943-951

National Engineering Research Center of Edible Fungi, Key Laboratory of Applied Mycological Resources and Utilization of Ministry of Agriculture, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.

In recent years, gut microbiota have been linked to prevention and treatment of human diseases. Mushrooms are a source of potentially useful prebiotics because they contain polysaccharides, terpenoids, and other bioactive compounds. In the present review, we have summarized the prebiotic effects of mushrooms on gut microbiota in the context of immunological, metabolic, neurological, and cancer-related diseases in the last five years. We propose that mushrooms can not only change the composition of gut microbiota, but also promote secretion of beneficial metabolites. In addition, we point to the effects of host mRNA expression in gut microbiota as a direction of further study. Overall, these provide a background for further studies on the mechanisms of regulation of gut microbiota by mushrooms.
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http://dx.doi.org/10.1615/IntJMedMushrooms.2020035799DOI Listing
January 2020

COVID-19-related myocarditis and cholinergic anti-inflammatory pathways.

Hellenic J Cardiol 2020 Dec 8. Epub 2020 Dec 8.

Department of Cardiology, Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325000, China. Electronic address:

Coronavirus disease-2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus-2, is currently in a pandemic outbreak and has become a global health issue. In addition to the primarily involvement of the respiratory system, myocarditis is considered an important and fatal lesion in patients with COVID-19. However, effective therapeutic methods are currently lacking. The cholinergic anti-inflammatory pathway (CAP) has been demonstrated to suppress pro-inflammatory cytokine production and control inflammation in sepsis and other medical conditions. Therefore, the CAP may be a potential and effective therapeutic method for COVID-19-related myocarditis. This article reviews the relationship between COVID-19-related myocarditis and the CAP and discusses the CAP as a potential therapeutic modality in the treatment of COVID-19-related myocarditis.
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http://dx.doi.org/10.1016/j.hjc.2020.12.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7722500PMC
December 2020

Discrimination of Thermophilic Proteins and Non-thermophilic Proteins Using Feature Dimension Reduction.

Front Bioeng Biotechnol 2020 22;8:584807. Epub 2020 Oct 22.

Information and Computer Engineering College, Northeast Forestry University, Harbin, China.

Thermophilicity is a very important property of proteins, as it sometimes determines denaturation and cell death. Thus, methods for predicting thermophilic proteins and non-thermophilic proteins are of interest and can contribute to the design and engineering of proteins. In this article, we describe the use of feature dimension reduction technology and LIBSVM to identify thermophilic proteins. The highest accuracy obtained by cross-validation was 96.02% with 119 parameters. When using only 16 features, we obtained an accuracy of 93.33%. We discuss the importance of the different characteristics in identification and report a comparison of the performance of support vector machine to that of other methods.
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http://dx.doi.org/10.3389/fbioe.2020.584807DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7642589PMC
October 2020

Assessing the effectiveness of statin therapy for alleviating cerebral small vessel disease progression in people ≥75 years of age.

BMC Geriatr 2020 08 17;20(1):292. Epub 2020 Aug 17.

Basic Medical College, Shandong First Medical University, Jinan, 250062, Shandong, China.

Background: Statins have been recommended by several guidelines as the primary prevention medication for cardiovascular diseases. However, the benefits of statin therapy for cerebral small vessel disease (CSVD), particularly in adults ≥75 years of age, have not been fully evaluated.

Methods: We analyzed the data from a prospective population-based cohort study and a randomized, double-blind, placebo-controlled clinical trial to determine whether statin therapy might aid in slowing the progression of CSVD in adults ≥75 years of age. For the cohort study, 827 participants were considered eligible and were included in the baseline analysis. Subsequently, 781 participants were included in follow-up analysis. For the clinical trial, 227 participants were considered eligible and were used in the baseline and follow-up analyses.

Results: The white matter hyperintensities (WMH) volume, the WMH-to-intracranial volume (ICV) ratio, the prevalence of a Fazekas scale score ≥ 2, lacunes, enlarged perivascular spaces (EPVS), and microbleeds were significantly lower in the statin group than the non-statin group at baseline in the cohort study (all P < 0.05). During the follow-up period, in both the cohort and clinical trial studies, the WMH volume and WMH-to-ICV ratio were significantly lower in the statin/rosuvastatin group than the non-statin/placebo group (all P < 0.001). Statin therapy was associated with lower risk of WMH, lacunes, and EPVS progression than the non-statin therapy group after adjustment for confounders (all P < 0.05). There was no statistically significant difference in the risk of microbleeds between the statin and non-statin therapy groups (all, P > 0.05).

Conclusions: Our findings indicated that statin therapy alleviated the progression of WMH, lacunes, and EPVS without elevating the risk of microbleeds. On the basis of the observed results, we concluded that statin therapy is an efficient and safe intervention for CSVD in adults ≥75 years of age.

Trial Registration: Chictr.org.cn: ChiCTR-IOR-17013557 , date of trial retrospective registration November 27, 2017 and ChiCTR-EOC-017013598 , date of trial retrospective registration November 29, 2017.
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http://dx.doi.org/10.1186/s12877-020-01682-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7430010PMC
August 2020

Transcriptome analysis of table grapes (Vitis vinifera L.) identified a gene network module associated with berry firmness.

PLoS One 2020 17;15(8):e0237526. Epub 2020 Aug 17.

College of Horticulture, Shenyang Agricultural University, Shenyang, Liaoning, P.R. China.

Berry firmness is one of the main selection criteria for table grape breeding. However, the underlying genetic determinants and mechanisms involved in gene expression during berry development are still poorly understood. In this study, eighteen libraries sampled from Vitis vinifera L. cv. 'Red Globe' and 'Muscat Hamburg' at three developmental stages (preveraison, veraison and maturation) were analyzed by RNA sequencing (RNA-Seq). The firmness of 'Red Globe' was significantly higher than that of 'Muscat Hamburg' at the three developmental stages. In total, a set of 4,559 differentially expressed genes (DEGs) was identified between 'Red Globe' and 'Muscat Hamburg' in the preveraison (2,259), veraison (2030) and maturation stages (2682), including 302 transcription factors (TFs). Weighted gene coexpression network analysis (WGCNA) showed that 23 TFs were predicted to be highly correlated with fruit firmness and propectin content. In addition, the differential expression of the PE, PL, PG, β-GAL, GATL, WAK, XTH and EXP genes might be the reason for the differences in firmness between 'Red Globe' and 'Muscat Hamburg'. The results will provide new information for analysis of grape berry firmness and softening.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0237526PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7430731PMC
October 2020

Role of TNF-α and FGF-2 in the Fracture Healing Disorder of Type 2 Diabetes Model Induced by High Fat Diet Followed by Streptozotocin.

Diabetes Metab Syndr Obes 2020 30;13:2279-2288. Epub 2020 Jun 30.

Department of Orthopaedics and Traumatology, Third Xiangya Hospital of Central South University, Changsha 410013, Hunan Province, People's Republic of China.

Purpose: To investigate the effect of TNF-α and FGF-2 in the fracture healing disorder of type 2 diabetes.

Design/methodology/approach: Rat diabetes-bone traction model was established to investigate the effect of type 2 diabetes on the fracture healing and the association of TNF-α and FGF-2 with the process. Serological examination was performed to detect the related diabetes indexes. The proliferation activity of the cells was detected by MTT assay. The expressions of FGF-2 and TNF-α of osteoblasts in high glucose culture environment were detected by histochemistry and Western blotting.

Findings: Serological examination showed that in rats fed with high fat and sugar diet for 8 weeks, the serum total cholesterol (TC), triglyceride (TG), fasting insulin (FINs) significantly increased, but fasting blood glucose (FBG) had no significant change. Two weeks after intraperitoneal injection of STZ, rat serum TG, TC, and FBG increased significantly, while FINs did not change obviously. Two weeks after traction osteogenesis, X-ray examination and HE staining showed that the area of osteotylus in the diabetes group was significantly smaller than that in the control group. The number of PCNA positive cells in the osteotylus of diabetes group was significantly decreased. In the osteotylus of diabetes group, the expression of TNF-α was significantly increased and the expression of FGF-2 was significantly decreased. MTT assay showed that the proliferation activity of MC3T3-E1 cells in high glucose culture medium groups was significantly decreased at 24th hour of the culture, compared with the normal culture medium group. qPCR results showed that the expression of FGF-2 was significantly decreased while the expression of TNF-α was significantly increased in high glucose culture medium groups.

Originality/value: It was concluded that type 2 diabetes mellitus affected the fracture healing by causing osteoblast proliferation disorder. TNF-α and FGF-2 were important related factors for the process.
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http://dx.doi.org/10.2147/DMSO.S231735DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7335275PMC
June 2020

Ultrafast Encapsulation of Metal Nanoclusters into MFI Zeolite in the Course of Its Crystallization: Catalytic Application for Propane Dehydrogenation.

Angew Chem Int Ed Engl 2020 Oct 13;59(44):19669-19674. Epub 2020 Aug 13.

Department of Chemical System Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan.

Encapsulating metal nanoclusters into zeolites combines the superior catalytic activity of the nanoclusters with high stability and unique shape selectivity of the crystalline microporous materials. The preparation of such bifunctional catalysts, however, is often restricted by the mismatching in time scale between the fast formation of nanoclusters and the slow crystallization of zeolites. We herein demonstrate a novel strategy to overcome the mismatching issue, in which the crystallization of zeolites is expedited so as to synchronize it with the rapid formation of nanoclusters. The concept was demonstrated by confining Pt and Sn nanoclusters into a ZSM-5 (MFI) zeolite in the course of its crystallization, leading to an ultrafast, in situ encapsulation within just 5 min. The Pt/Sn-ZSM-5 exhibited exceptional activity and selectivity with stability in the dehydrogenation of propane to propene. This method of ultrafast encapsulation opens up a new avenue for designing and synthesizing composite zeolitic materials with structural and compositional complexity.
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http://dx.doi.org/10.1002/anie.202007044DOI Listing
October 2020

Telmisartan and Rosuvastatin Synergistically Ameliorate Dementia and Cognitive Impairment in Older Hypertensive Patients With Apolipoprotein E Genotype.

Front Aging Neurosci 2020 9;12:154. Epub 2020 Jun 9.

Institute of Basic Medicine, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China.

: To investigate the effect of telmisartan, rosuvastatin, or their combination on dementia and to understand the impact of () genotype on the effect of the medications in older patients with hypertension. : This is a double-blind, randomized, and placebo-controlled trial using a 2 × 2 factorial design. Between April 2008 and November 2010, 1,244 hypertensive patients aged ≥60 years without cognitive impairment were recruited from communities in six cities in Shandong area, China. Patients were randomized into telmisartan and rosuvastatin administration after a 2-week washout period. genotype was identified at the baseline. Possible dementia was determined using the combination of the global cognitive function and Assessment of the Informant Questionnaire on Cognitive Decline in the Elderly (IQCODE). : Over an average follow-up of 7 [interquartile range (IQR): 6.7-7.2] years, telmisartan and rosuvastatin significantly reduced the cognitive impairment progression and the incidence of dementia. There was a synergistic interaction between telmisartan and rosuvastatin to reduce the cognitive impairment and the incidence of dementia ( < 0.001). The cognitive impairment progression and the risk of dementia were higher in the hypertensive patients with allele than in those without allele. Rosuvastatin medication significantly alleviated the cognitive impairment progression and the risks of dementia in patients with allele. : The combination of telmisartan and rosuvastatin might be an effective prevention and/or treatment strategy for cognitive impairment and dementia, especially in hypertensive patients with the allele. www.ClinicalTrials.gov, ChiCTR.org.cn, identifier ChiCTR-IOR-17013557. Registered on April 12, 2017 - Retrospectively registered, http://www.chictr.org.cn/showproj.aspx?proj=23121.
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http://dx.doi.org/10.3389/fnagi.2020.00154DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7296075PMC
June 2020

High-density genetic linkage map construction and cane cold hardiness QTL mapping for Vitis based on restriction site-associated DNA sequencing.

BMC Genomics 2020 Jun 22;21(1):419. Epub 2020 Jun 22.

College of Horticulture, Shenyang Agricultural University, Shenyang, P.R. China.

Background: Cold hardiness is an important agronomic trait and can significantly affect grape production and quality. Until now, there are no reports focusing on cold hardiness quantitative trait loci (QTL) mapping. In this study, grapevine interspecific hybridisation was carried out with the maternal parent 'Cabernet sauvignon' and paternal parent 'Zuoyouhong'. A total of 181 hybrid offspring and their parents were used as samples for restriction-site associated DNA sequencing (RAD). Grapevine cane phloem and xylem cold hardiness of the experimental material was detected using the low-temperature exotherm method in 2016, 2017 and 2018. QTL mapping was then conducted based on the integrated map.

Results: We constructed a high-density genetic linkage map with 16,076, 11,643, and 25,917 single-nucleotide polymorphism (SNP) markers anchored in the maternal, paternal, and integrated maps, respectively. The average genetic distances of adjacent markers in the maps were 0.65 cM, 0.77 cM, and 0.41 cM, respectively. Colinearity analysis was conducted by comparison with the grape reference genome and showed good performance. Six QTLs were identified based on the phenotypic data of 3 years and they were mapped on linkage group (LG) 2, LG3, and LG15. Based on QTL results, candidate genes which may be involved in grapevine cold hardiness were selected.

Conclusions: High-density linkage maps can facilitate grapevine fine QTL mapping, genome comparison, and sequence assembly. The cold hardiness QTL mapping and candidate gene discovery performed in this study provide an important reference for molecular-assisted selection in grapevine cold hardiness breeding.
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http://dx.doi.org/10.1186/s12864-020-06836-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7310074PMC
June 2020

Abnormally high expression of HOXA2 as an independent factor for poor prognosis in glioma patients.

Cell Cycle 2020 07 21;19(13):1632-1640. Epub 2020 May 21.

Department of Orthopaedics, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, School of Clinical Medicine, Henan University , Zhengzhou, Henan, China.

In recent years, studies have revealed HOXA2 as a new oncogene, but its function is unknown in gliomas. We aimed to reveal the relationship between HOXA2 and glioma based on the Chinese Glioma Genome Atlas(CGGA) and the cancer genome atlas (TCGA). HOXA2 expression data and clinically relevant information of glioma patients were obtained from the CGGA and TCGA containing 1447 glioma tissues and five non-tumor brain tissues. The Wilcox or Kruskal tests were used to detect the correlation between the HOXA2 expression level and clinical data of glioma patients. the Kaplan-Meier method were used to examine the relationship between HOXA2 and overall patient survival. Gene set enrichment analysis (GSEA) was conducted to indirectly reveal the signaling pathways involved in HOXA2, and RT-PCR was used to detect HOXA2 expression in gliomas and non-tumor brain tissues. High HOXA2 expression was found to be positively correlated with clinical grade, histological type, age, and tumor recurrence, but negatively correlated with 1p19 codeletion and isocitrate dehydrogenase mutation status.RT-PCR results showed that HOXA2 expression levels were significantly higher in tumor tissues than in non-tumor brain tissues. GSEA showed that HOXA2 promoted the activation of the activation of the JAK-STAT-signaling pathway, focal adhesion, cell-adhesion-molecules-CAMS pathway, cytosolic DNA sensing pathway, and natural killer cell-mediated cytotoxicity. This study revealed for the first time that the novel oncogene,HOXA2, leads to poor prognosis in gliomas, and can be used as a biomarker for the diagnosis and treatment of gliomas.
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http://dx.doi.org/10.1080/15384101.2020.1762038DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7469623PMC
July 2020

Identification of hub genes and small-molecule compounds in medulloblastoma by integrated bioinformatic analyses.

PeerJ 2020 14;8:e8670. Epub 2020 Apr 14.

Department of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, People's Republic of China.

Background: Medulloblastoma (MB) is the most common intracranial malignant tumor in children. The genes and pathways involved in the pathogenesis of MB are relatively unknown. We aimed to identify potential biomarkers and small-molecule drugs for MB.

Methods: Gene expression profile data sets were obtained from the Gene Expression Omnibus (GEO) database and the differentially expressed genes (DEGs) were identified using the Limma package in R. Functional annotation, and cell signaling pathway analysis of DEGs was carried out using DAVID and Kobas. A protein-protein interaction network was generated using STRING. Potential small-molecule drugs were identified using CMap.

Result: We identified 104 DEGs (29 upregulated; 75 downregulated). Gene ontology analysis showed enrichment in the mitotic cell cycle, cell cycle, spindle, and DNA binding. Cell signaling pathway analysis identified cell cycle, HIF-1 signaling pathway, and phospholipase D signaling pathway as key pathways. SYN1, CNTN2, FAIM2, MT3, and SH3GL2 were the prominent hub genes and their expression level were verified by RT-qPCR. Vorinostat, resveratrol, trichostatin A, pyrvinium, and prochlorperazine were identified as potential drugs for MB. The five hub genes may be targets for diagnosis and treatment of MB, and the small-molecule compounds are promising drugs for effective treatment of MB.

Conclusion: In this study we obtained five hub genes of MB, SYN1, CNTN2, FAIM2, MT3, and SH3GL2 were confirmed as hub genes. Meanwhile, Vorinostat, resveratrol, trichostatin A, pyrvinium, and prochlorperazine were identified as potential drugs for MB.
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http://dx.doi.org/10.7717/peerj.8670DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7164431PMC
April 2020

Construction of lncRNA-associated ceRNA networks to identify prognostic lncRNA biomarkers for glioblastoma.

J Cell Biochem 2020 07 10;121(7):3502-3515. Epub 2020 Apr 10.

Department of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China.

Long noncoding RNAs (lncRNAs) serve as competitive endogenous RNAs (ceRNAs) that play significant regulatory roles in the pathogenesis of tumors. However, the role of lncRNAs, especially the lncRNA-related ceRNA regulatory network, in glioblastoma (GBM) has not been fully elucidated. The goal of the current study was to construct lncRNA-microRNA-mRNA-related ceRNA networks for further investigation of their mechanism of action in GBM. We downloaded data from The Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus (GEO) databases and identified differential lncRNAs, microRNAs (miRNAs), and messenger RNAs (mRNAs) associated with GBM. A ceRNA network was constructed and analyzed to examine the relationship between lncRNAs and patients' overall survival. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGGs) were used to analyze the related mRNAs to indirectly explain the mechanism of action of lncRNAs. The potential effective drugs for the treatment of GBM were identified using the connectivity map (CMap). After integrated analysis, we obtained a total of 210 differentially expressed lncRNAs, 90 differentially expressed miRNAs, and 2508 differentially expressed mRNAs (DEmRNAs) from the TCGA and GEO databases. Using these differential genes, we constructed a lncRNA-associated ceRNA network. Six lncRNAs in the ceRNA network were associated with the overall survival of patients with GBM. Through KEGG analysis, it was found that the DEmRNAs involved in the network are related to cancer-associated pathways, for instance, mitogen-activated protein kinase and Ras signaling pathways. CMap analysis revealed four small-molecule compounds that could be used as drugs for the treatment of GBM. In this study, a multi-database joint analysis was used to construct a lncRNA-related ceRNA network to help identify the regulatory functions of lncRNAs in the pathogenesis of GBM.
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http://dx.doi.org/10.1002/jcb.29625DOI Listing
July 2020

Home-measured orthostatic hypotension associated with cerebral small vessel disease in a community-based older population.

Hypertens Res 2020 08 18;43(8):798-807. Epub 2020 Mar 18.

Shandong Provincial Hospital Affiliated with Shandong First Medical University, Jinan, Shandong, 250062, China.

This study aimed to investigate the relationship between cerebral small vessel disease (CSVD) and orthostatic hypotension (OH) using self-measured blood pressure at home in community-dwelling older subjects. Between May 2016 and October 2018, 663 community-dwelling adults aged ≥60 years were enrolled in Shandong, China. CSVD, including white matter hyperintensities (WMHs), lacunes, enlarged Virchow-Robin spaces (EVRS) and microbleeds, was assessed using brain magnetic resonance imaging. After receiving appropriate training, the subjects participated in "home-measured (H)OH" by themselves for three consecutive days. Participants were classified into no-HOH, 1 HOH, and ≥2 HOH episode groups according to the presence of HOH episodes. The WMH volume, WMH-to-total intracranial volume (TIV) ratio, total numbers of lacunes and EVRS, and prevalence of Fazekas scale score ≥2, lacunes, and EVRS were elevated in the 1 and ≥2 HOH episode groups compared with the no-HOH episode group (P < 0.05). The prevalence and total number of microbleeds were significantly higher in the ≥2 HOH episodes group than in the no-HOH and 1 HOH episode groups (P < 0.05). HOH episodes were significantly associated with WMH volume, WMH-to-TIV ratio, and the total numbers of lacunes, EVRS, and microbleeds after adjustment for confounders (P < 0.05). The risks of Fazekas scale score ≥2, lacunes, EVRS, and microbleeds were 2.123-, 1.893-, 2.162-, and 1.656-fold higher in the 1 HOH episode group and 4.910-, 5.359-, 3.048-, and 2.418-fold higher in the ≥2 HOH episodes group, respectively, than those in the no-HOH group. The presence of HOH episodes was an independent risk factor for CSVD in the community-based older population.
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http://dx.doi.org/10.1038/s41440-020-0429-xDOI Listing
August 2020

A Novel Shell Material-Highland Barley Starch for Microencapsulation of Cinnamon Essential Oil with Different Preparation Methods.

Materials (Basel) 2020 Mar 6;13(5). Epub 2020 Mar 6.

Food Science College, TAAHC-SWU Medicinal Plants Joint Research and Development Centre, Tibet Agriculture & Animal Husbandry University, Nyingchi 860000, China.

Highland barley starch (HBS), as a carbohydrate shell material with excellent performance in microcapsule applications, has rarely been reported. In the present study, three different microcapsules (CEO-SWSM, CEO-PM, and CEO-UM) were synthesized successfully via saturated aqueous solution method, molecular inclusion method and ultrasonic method, respectively, using HBS as shell material coupled with cinnamon essential oil (CEO) as the core material. The potential of HBS as a new shell material and the influence of synthetic methods on the performance of microcapsules, encapsulation efficiency (EE), yield, and release rate of CEO-SWSM, CEO-PM, and CEO-UM were determined, respectively. The results confirmed that CEO-PM had the most excellent EE (88.2%), yield (79.1%), as well as lowest release rate (11.5%, after 25 days of storage). Moreover, different kinetic models were applied to fit the release process of these three kinds of microcapsules: CEO-SWSM, CEO-PM, and CEO-UM had the uppermost R-squared value in the Higuchi model, the zero-order model, and the first-level model, respectively. Over all, this work put forward a novel perspective for the improved encapsulation effect of perishable core materials (e.g., essential oil) for the food industry.
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http://dx.doi.org/10.3390/ma13051192DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7085060PMC
March 2020

Unique crystallization behavior in zeolite synthesis under external high pressures.

Chem Commun (Camb) 2020 Mar 7;56(18):2811-2814. Epub 2020 Feb 7.

Department of Chemical System Engineering, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.

The function of pressure has long been overlooked in the hydrothermal synthesis of zeolites, which is typically carried out under an autogenous pressure (below 2 MPa). We herein report, the first of its kind, a detailed investigation on the hydrothermal synthesis of zeolites under external high pressures, where crystallization behaviors contradicting the common observations were generated.
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http://dx.doi.org/10.1039/c9cc09966bDOI Listing
March 2020

Preparation and electrochemical performance of P-doped LiTiO as anode material for lithium-ion batteries.

Nanotechnology 2020 May 27;31(20):205402. Epub 2020 Jan 27.

College of Materials and Chemistry & Chemical Engineering, Chengdu University of Technology, Chengdu 610059, People's Republic of China.

LiTi P O (x = 0, 0.1, 0.2, 0.3, 0.4, 0.5) as an anode material was synthesized using a sol-gel method with Ti(CHO), LiCO, and NHHPO, followed by a calcination treatment. The influences of P doping on the crystal structure, phase composition, and morphology of LiTi P O were analyzed by x-ray diffraction and scanning electron microscopy. The electrochemical properties of LiTi P O were characterized using galvanostatic charge-discharge cycles, cyclic voltammetry, and alternating current impedance experiments. The results indicate that when x = 0.2, the LiTiPO sample exhibits the best initial discharge specific capacity of 132.1 mAh g at first cycle, and the discharge specific capacity remains at 128.3 mAh g after 500 cycles.
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http://dx.doi.org/10.1088/1361-6528/ab7047DOI Listing
May 2020

Candidate genes for grape white rot resistance based on SMRT and Illumina sequencing.

BMC Plant Biol 2019 Nov 15;19(1):501. Epub 2019 Nov 15.

College of Horticulture, Shenyang Agricultural University, Shenyang, 110866, China.

Background: White rot is one of the most dangerous fungal diseases and can considerably affect grape berry production and quality. However, few studies have focused on this disease, and thus, finding candidate white rot resistance genes is of great importance for breeding resistant grapevine cultivars. Based on field observations and indoor experiments, the cultivars "Victoria" and "Zhuosexiang" showed significant differences in white rot resistance. For understanding the molecular mechanisms behind it, different phenotypes of grapevine leaves were used for RNA sequencing via Illumina and single-molecule real-time (SMRT) sequencing technology.

Results: A transcript library containing 53,906 reads, including known and novel transcripts, was constructed following the full-length transcriptome sequencing of the two grapevine cultivars. Genes involved in salicylic acid (SA) and jasmonic acid (JA) synthesis pathways showed different expression levels. Furthermore, four key transcription factors (TFs), NPR1, TGA4, Pti6, and MYC2, all involved in the SA and JA signal pathways were identified, and the expression profile revealed the different regulation of the pathogenesis related protein1 (PR1) resistance gene, as mediated by the four TFs.

Conclusions: Full-length transcript sequencing can substantially improve the accuracy and integrity of gene prediction and gene function research in grapevine. Our results contribute to identify candidate resistance genes and improve our understanding of the genes and regulatory mechanisms involved in grapevine resistance to white rot.
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http://dx.doi.org/10.1186/s12870-019-2119-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6858721PMC
November 2019