Publications by authors named "Zhen-Wei Yang"

3 Publications

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A Stereological Study of Mouse Ovary Tissues for 3D Bioprinting Application.

Cell Mol Bioeng 2021 Jun 15;14(3):259-265. Epub 2021 Mar 15.

Department of Gynecology, Second Hospital, Hebei Medical University, No.215, He Ping Road (West), Xin Hua District, Shijiazhuang, Hebei Province China.

Introduction: The use of 3D-bioprinted ovaries has been proven to be a promising technique for preserving fertility. Stereology is an accurate method to obtain quantitative 3D information and the stereological data is the basis for 3D bioprinting ovaries.

Methods: In this study, six female mice were used to acquire the ovarian tissues. One of the two paraffin-embedded ovaries of each mouse was cut into 5 m sections, and the other was cut into 15 m sections and then subjected to haematoxylin and eosin staining and anti-follicle stimulating hormone receptor antibody immunohistochemistry. The volume and volume fractions of ovaries were measured by the Cavalieri method. Then, the numerical densities and total numbers of ovarian granulosa cells (OGCs) and primordial, preantral and antral follicles in serial sections were estimated using design-based stereology.

Results: The ovarian volume was 2.50 ± 0.32 mm. The volume fractions of the cortex, medulla, follicles and OGCs were 86.80% ± 2.82, 13.20% ± 2.82%, 5.60% ± 0.25% and 81.19% ± 2.57%, respectively. The numerical densities of OGCs, the primordial, preantral and antral follicles were 2.11 (± 0.28) × 10/mm, 719.57 ± 18.04/mm, 71.84 ± 3.93/mm and 17.29 ± 3.54/mm, respectively. The total number of OGCs and follicles per paraffin-embedded ovary were 5.26 (± 0.09) × 10 and 2013.66 ± 8.16.

Conclusions: The study had obtained the stereological data of the mice ovaries, which contribute to a deeper understanding of the structure of the ovaries. Meanwhile, the data will supply information for 3D bioprinting ovaries.
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June 2021

Rapid and Quantitative Detection of Aflatoxin B in Grain by Portable Raman Spectrometer.

Appl Spectrosc 2020 Nov 27;74(11):1365-1373. Epub 2020 Aug 27.

State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, 12466Xiamen University, Xiamen, China.

Many foodstuffs are extremely susceptible to contamination with aflatoxins, in which aflatoxin B is highly toxic and carcinogenic. Therefore, it is crucial to develop a rapid and effective analytical method for detecting and monitoring aflatoxin B in food. Herein, a surface-enhanced Raman spectroscopic (SERS) method combined with QuEChERS (quick, easy, cheap-effective, rugged, safe) sample pretreatment technique was used to detect aflatoxin B. Sample preparation was optimized into a one-step extraction method using an Au nanoparticle-based solution (Au sol) as the SERS detection substrate. An affordable portable Raman spectrometer was then used for rapid, label-free, quantitative detection of aflatoxin B levels in foodstuffs. This method showed a good linear log relationship between the Raman signal intensity of aflatoxin B in the 1-1000 µg L concentration range with a limit of detection of 0.85 µg kg and a correlation coefficient of 0.9836. Rapid aflatoxin B detection times of ∼10 min for wheat, corn, and protein feed powder samples were also achieved. This method has high sensitivity, strong specificity, excellent stability, is simple to use, economical, and is suitable for on-site detection, with good prospects for practical application in the field of food safety.
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November 2020

Multimode quantum states with single photons carrying orbital angular momentum.

Sci Rep 2017 06 15;7(1):3601. Epub 2017 Jun 15.

Beijing Key Laboratory of Nanophotonics & Ultrafine Optoelectronic Systems, School of Physics, Beijing Institute of Technology, Beijing, 100081, China.

We propose and experimentally demonstrate a scheme for generating multimode quantum states with single photons carrying orbital angular momentum (OAM). Various quantum states have been realized by superposing multiple OAM modes of single photons in two possible paths. These quantum states exhibit NOON-like "super-resolving" interference behavior for the multiple OAM modes of single photons. Compared with the NOON states using many photons, these states are not only easily prepared, but also robust to photon losses. They may find potential applications in quantum optical communication and recognizing defects or objects. The method to identify a particular kind of defect has been demonstrated both theoretically and experimentally.
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June 2017