Publications by authors named "Zhen Zheng"

171 Publications

Acupuncture as an Independent or Adjuvant Management to Standard Care for Perimenopausal Depression: A Systematic Review and Meta-Analysis.

Front Psychiatry 2021 28;12:666988. Epub 2021 May 28.

School of Health and Biomedical Sciences, RMIT University, Bundoora, VIC, Australia.

Many women with perimenopausal depression (PMD) have sought alternative therapies such as acupuncture because of concerns about risks associated with antidepressant and hormone replacement therapy (HRT). This systematic review aimed to clarify if acupuncture is effective for PMD compared with waitlist control or placebo/sham acupuncture, and if acupuncture alone or combined with standard care (antidepressant and/or HRT) is more effective in ameliorating PMD in comparison with standard care alone. Randomized controlled trials (RCTs) of PMD treatment acupuncture vs. waitlist control or placebo/sham acupuncture, and RCTs of PMD treatment acupuncture alone or combined with Western pharmacotherapy vs. Western pharmacotherapy were searched for from seven databases from inception to December 2020. Cochrane criteria were followed. Twenty-five studies involving 2,213 women were analyzed. Meta-analyses indicated that acupuncture significantly reduced the global scores of Hamilton Depression Scale (HAMD) [standardized mean difference (SMD) = -0.54, 95% CI (-0.91, -0.16), < 0.01], compared with standard care. The therapeutic effect of acupuncture maintained at 2-, 4-, and 12-week follow-ups. Acupuncture combined with standard care was more effective than standard care alone in decreasing HAMD scores [SMD = -0.82, 95% CI (-1.07, -0.58), < 0.01]. Too few RCTs were available to assess the clinical efficacy differences between acupuncture and placebo/sham acupuncture or HRT alone. Acupuncture also showed better effects in decreasing Kupperman index (KI) scores, whether compared with antidepressant alone [MD = -4.55, 95% CI (-8.46, -0.65), = 0.02] or antidepressant combined with HRT [MD = -0.89, 95% CI (-1.34, -0.43), < 0.01]. In comparison with standard care, acupuncture alone or combined with standard care was associated with significant improvements in PMD and reductions of other menopausal symptoms. This finding suggests that acupuncture may be a useful addition to treatment for PMD.
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http://dx.doi.org/10.3389/fpsyt.2021.666988DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8192720PMC
May 2021

Baseline Serum Cholesterol Levels Predict the Response of Patients with Advanced Non-Small Cell Lung Cancer to Immune Checkpoint Inhibitor-Based Treatment.

Cancer Manag Res 2021 18;13:4041-4053. Epub 2021 May 18.

Department of Radiation Oncology, Ningbo Medical Treatment Center Li Huili Hospital, Ningbo, 315040, People's Republic of China.

Purpose: Although predictive markers of immune checkpoint inhibitor (ICI)-based treatments have been extensively studied, with the exception of programmed death ligand 1 (PD-L1), most are not widely used in the clinic due to poor effects or defective practicability. The aim of this study was to identify those patients with high baseline serum cholesterol who benefit from ICI-based treatments.

Patients And Methods: Patients with advanced non-small cell lung cancer (NSCLC) treated at Ningbo Medical Center, Li Huili Hospital between August 2017 and December 2019 were enrolled in this retrospective study. The Response Evaluation Criteria in Solid Tumors (RECIST, version 1.1) were used to evaluate the efficacy of the ICI-based treatment. Progression-free survival (PFS) and overall survival (OS) were calculated using the Kaplan-Meier survival curves and compared using the log rank test. Univariate and multivariate analyses were conducted using the logistic regression analysis and Cox proportional hazards model. A receiver operating characteristic curve was created, and the area under the curve (AUC) was calculated to compare the predictive value of baseline serum cholesterol with PD-L1 expression for patient response to ICI-based treatment.

Results: In our cohort of 169 NSCLC patients, the objective response rate (ORR) and disease control rate (DCR) of the treatment were significantly higher in patients with hypercholesterolemia (>5.18 mmol/L) than in those with hypocholesterolemia (ORR: 33.67% vs 14.08%, P=0.004; DCR: 68.37% vs 42.25%, P=0.001). The median PFS was 7.9 months in the hypercholesterolemia group, significantly longer than in the hypocholesterolemia group (4.4 months, 95% CI: 4.620-7.380, P<0.001). The median OS in the two groups were 11 months and 8 months, with 95% CIs of 8.980-10.420 (P<0.001). The AUC for the baseline level of cholesterol was 0.706 (P<0.001), while it was 0.643 (P=0.001) for PD-L1 expression.

Conclusion: The baseline serum cholesterol level is predictive of a clinical benefit for advanced NSCLC patients who undergo ICI-based treatment, and hence it is a promising prognostic indicator for ICI-based treatment of NSCLC.
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http://dx.doi.org/10.2147/CMAR.S304022DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8140899PMC
May 2021

Comparative Utility of Acupuncture and Western Medication in the Management of Perimenopausal Insomnia: A Systematic Review and Meta-Analysis.

Evid Based Complement Alternat Med 2021 26;2021:5566742. Epub 2021 Apr 26.

School of Health and Biomedical Sciences, RMIT University, Bundoora, Victoria 3083, Australia.

Background: Many women with perimenopausal insomnia (PMI) have sought alternative therapies such as acupuncture because of concerns about risks associated with hormone replacement therapy (HRT) and/or psychotropic drugs. This systematic review aimed to clarify if acupuncture alone or combined with standard Western pharmacotherapy (HRT and/or psychotropic drugs) is more effective in ameliorating PMI in comparison to pharmacotherapy alone.

Methods: Randomized controlled trials (RCTs) of PMI treatment via acupuncture alone or combined with Western pharmacotherapy versus Western pharmacotherapy were searched for from eleven databases from inception to March 2020. Cochrane criteria were followed.

Results: Fifteen studies involving 1410 women were analyzed. Meta-analysis indicated that acupuncture significantly reduced the global scores of Pittsburgh Sleep Quality Index (PSQI) [MD = -2.38, 95% CI (-3.38, -1.37),  < 0.01] and Kupperman Index [MD = -5.95, 95% CI (-10.68, -1.21),  = 0.01], compared with hypnotics. Acupuncture combined with hypnotics was more effective than hypnotics alone in decreasing PSQI scores [MD = -3.13, 95% CI (-5.43, -0.83),  < 0.01]. Too few RCTs were available to investigate the clinical efficacy differences between acupuncture and HRT/psychotropic drugs other than hypnotics.

Conclusions: Despite limited evidence, in comparison to hypnotics, acupuncture was associated with significant improvements in PMI, and reductions of other menopausal symptoms. This finding suggests that acupuncture may be a useful addition to treatment for PMI.
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http://dx.doi.org/10.1155/2021/5566742DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8093060PMC
April 2021

Advances and Insights of APC-Asef Inhibitors for Metastatic Colorectal Cancer Therapy.

Front Mol Biosci 2021 22;8:662579. Epub 2021 Apr 22.

Department of Pathophysiology, Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

In Colorectal cancer (CRC), adenomatous polyposis coli (APC) directly interacts with the Rho guanine nucleotide exchange factor 4 (Asef) and releases its GEF activity. Activated Asef promotes the aberrant migration and invasion of CRC cell through a CDC42-mediated pathway. Knockdown of either APC or Asef significantly decreases the migration of CRC cells. Therefore, disrupting the APC-Asef interaction is a promising strategy for the treatment of invasive CRC. With the growth of structural information, APC-Asef inhibitors have been designed, providing hope for CRC therapy. Here, we will review the APC-Asef interaction in cancer biology, the structural complex of APC-Asef, two generations of peptide inhibitors of APC-Asef, and small molecule inhibitors of APC-Asef, focusing on research articles over the past 30 years. We posit that these advances in the discovery of APC-Asef inhibitors establish the protein-protein interaction (PPI) as targetable and provide a framework for other PPI programs.
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http://dx.doi.org/10.3389/fmolb.2021.662579DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100458PMC
April 2021

Healthcare Professionals' Attitudes to Integration of Acupuncture in Western Medicine: A Mixed-Method Systematic Review.

Pain Manag Nurs 2021 May 4. Epub 2021 May 4.

School of Health and Biomedical Sciences, RMIT University, Bundoora West Campus, Australia.

Background: Acupuncture is practiced in many Western countries with increasing evidence supporting its use across the healthcare system. However, this nonpharmacological intervention is yet to be widely integrated into hospitals. Fundamental to the integration of any innovation into healthcare systems is the attitudes of healthcare professionals.

Aims: To explore healthcare professionals' attitudes towards acupuncture and identify the enablers and barriers to integration in the hospital setting.

Design: This review was reported according to the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guidelines; we conducted a mixed-method systematic review following Joanna Briggs Institute Reviewer's Manual 2017.

Data Sources: PubMed, CINAHL, AMED, EMBASE, Cochrane Library, and SCOPUS were searched from their inception to December 2019 using different combination of keywords.

Methods: Attitude and perception data were extracted from the articles and constructs were categorized as barriers and enablers. A convergent integrated approach was adopted whereby qualitative data themes were integrated with quantitative data constructs to generate descriptive codes around enablers and barriers at intrapersonal, interpersonal, and environmental levels according to the framework proposed by Manias et al. (2014).

Results: Twenty-six studies comprising 19 survey and seven interview studies were identified, with 24 articles (92%) reporting positive attitudes, supporting the integration of acupuncture into Western medicine. Enablers identified were prior positive experience, belief in the treatment's efficacy, and patient demand. Barriers were lack of evidentiary knowledge or experience; lack of resources such as time, providers, and funding; and paradigm differences. Twenty studies (77%) focused on medical doctors or medical student perceptions. A conceptual framework for implementing and integrating acupuncture into Western medicine is proposed.

Conclusion: Healthcare professionals' attitudes to acupuncture are positive. Barriers to integrating acupuncture into the hospital system exist at intrapersonal, interpersonal, and external levels, with lack of resources being the key barrier. Nurses' role in facilitating integration into Western medical pain management practice provides an avenue for future research. The proposed conceptual framework provides guidance for nursing researchers interested in the role of acupuncture and integrated medicine in patient-centric, value-based healthcare.
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http://dx.doi.org/10.1016/j.pmn.2021.03.010DOI Listing
May 2021

Nucleation and growth dynamics of graphene grown by radio frequency plasma-enhanced chemical vapor deposition.

Sci Rep 2021 Mar 16;11(1):6007. Epub 2021 Mar 16.

Key Laboratory of Advanced Corrosion and Protection for Aviation Materials, Beijing Institute of Aeronautical Materials, Aero Engine Corporation of China, Beijing, 10095, China.

We investigated the nucleation and grain growth of graphene grown on Cu through radio frequency plasma-enhanced chemical vapor deposition (RF-PECVD) at different temperatures. A reasonable shielding method for the placement of copper was employed to achieve graphene by RF-PECVD. The nucleation and growth of graphene grains during PECVD were strongly temperature dependent. A high growth temperature facilitated the growth of polycrystalline graphene grains with a large size (~ 2 μm), whereas low temperature induced the formation of nanocrystalline grains. At a moderate temperature (790 to 850 °C), both nanocrystalline and micron-scale polycrystalline graphene grew simultaneously on Cu within 60 s with 50 W RF plasma power. As the growth time increased, the large graphene grains preferentially nucleated and grew rapidly, followed by the nucleation and growth of nanograins. There was competition between the growth of the two grain sizes. In addition, a model of graphene nucleation and grain growth during PECVD at different temperatures was established.
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http://dx.doi.org/10.1038/s41598-021-85537-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7966375PMC
March 2021

Bio-based visual optical pressure-responsive sensor.

Carbohydr Polym 2021 May 16;260:117823. Epub 2021 Feb 16.

School of Chemistry and Chemical Engineering, State Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University, Shanghai, 200240, PR China. Electronic address:

A bio-based pressure-responsive sensor with adjustable structural color is prepared by combining aerogel skeleton of cellulose nanocrystals (CNCs)/poly(ethylene glycol) (PEG) obtained via the ice-templating method with flexible polyacrylamide (PAAM) elastomer. The white aerogel is composed of consecutive ribbons, demonstrating chiral nematic structure. These ribbons are rearranged to be vertical to the force direction, leading to immediate appearance of the structural color when the 3D aerogel transforms to a 2D plane. Helical pitches are regulated by the PEG content that the wavelength of structural color covers up to 178 nm. There is an excellent linear correlation between pressure and transmittance of reflectance peak, and the sensitivity to pressure can be regulated by changing solid content of PAAM. Furthermore, the pressure-responsive color is still vivid after 16 cycles of compression. This flexible material with pressure-responsive structural color is promising in sensing, intelligent display, information transmission, and etc.
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http://dx.doi.org/10.1016/j.carbpol.2021.117823DOI Listing
May 2021

Can acupuncture improve objective sleep indices in patients with primary insomnia? A systematic review and meta-analysis.

Sleep Med 2021 Apr 2;80:244-259. Epub 2021 Feb 2.

School of Health and Biomedical Sciences, RMIT University, Bundoora, Victoria, 3083, Australia. Electronic address:

Background: The efficacy of acupuncture for treating primary insomnia (PI) has been explored in several meta-analyses where the outcomes measures were based on subjective self-reported sleep rating scales. Sleep state misperception is common in PI and self-reports underestimate sleep duration and quality. No systematic reviews or meta-analyses have investigated the efficacy of acupuncture in improving objective sleep measures in PI.

Objective: To perform a systematic review to determine whether objective sleep parameters are improved by acupuncture in patients with PI.

Methods: Randomized controlled trials treating PI via verum-acupuncture versus sham-/placebo-acupuncture or waitlist control were searched for in English [MEDLINE (via PubMed), Sciverse ScienceDirect, Cochrane Central Register of Controlled Trials (CENTRAL), AMED, Springer, EMBASE (Elsevier), Ebsco Medline, and PsycINFO (ProQuest)] and Chinese (SinoMed, CNKI, Wanfang and CQVIP) databases, from the dates of the databases' inception to January 2020. The parameters of polysomnography (PSG), actigraphy, or micromovement sensitive mattress/pillow sleep monitoring systems were considered as the primary outcome measures. Revman 5.3 and Stata 16.0 software were used to conduct the meta-analysis. Heterogeneity was examined by using I statistics and publication bias was assessed via Egger's test.

Results: Eleven studies involving 775 patients met the inclusion criteria. The results of meta-analysis suggested that acupuncture can increase total sleep time [MD = 55.29, 95%CI (29.16, 81.42), p < 0.01], increase sleep efficiency [MD = 8.96, 95%CI (3.97, 13.95), p < 0.01], decrease wake after sleep onset [MD = -49.54, 95%CI (-82.98, -16.09), p < 0.01], and reduce number of awakening times [MD = -6.29, 95%CI (-10.75, -1.82), p < 0.01] compared with either sham-/placebo-acupuncture or waitlist control. Subsequent analysis indicated a superior effect for verum-acupuncture in comparison with sham-/placebo-acupuncture or waitlist-control when the recommended lowest threshold dosage (12 sessions) was met. Despite positive outcomes, most studies reviewed were heterogeneous and at risk of bias due to methodological issues.

Conclusions: Despite limited evidence, acupuncture was significantly associated with improvements in several objective sleep parameters (increases in total sleep time and sleep efficiency, and reductions in wake after sleep onset and number of awakening times) as well as subjective sleep quantity and quality in patients with PI. A minimum therapeutic threshold dosage (≥12 sessions) is recommended. Well-designed RCTs using PSG are required to clarify the influence of acupuncture on sleep architecture/structure and to promote better application of acupuncture as a treatment for PI.
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http://dx.doi.org/10.1016/j.sleep.2021.01.053DOI Listing
April 2021

Optimization of Micafungin Dosage for Chinese Patients with Sepsis in the Intensive Care Unit Based on a Population Pharmacokinetic-Pharmacodynamic Analysis.

Pharm Res 2021 Jan 6;38(1):67-77. Epub 2021 Jan 6.

Department of Critical Care Medicine, Shengjing Hospital of China Medical University, Shenyang, Liaoning, China.

Purpose: This study aimed to identify parameters that influence micafungin pharmacokinetics in Chinese patients with sepsis in the intensive care unit and optimize micafungin dosage by determining the probability of reaching pharmacodynamic targets.

Methods: Blood samples were collected from 32 Chinese patients with sepsis who were treated with micafungin. The samples were analyzed and used to build a population pharmacokinetic model. Monte Carlo simulations were performed to estimate the probability of achieving adequate plasma levels of micafungin against Candida species.

Results: Alanine aminotransferase and sequential organ failure assessment score were found to significantly influence the clearance and peripheral distribution volume of micafungin, respectively. Monte Carlo simulations based on area under the plasma concentration-time curve over 24 h showed that patients must be administered at least 200 and 250 mg micafungin daily to reach minimum inhibitory concentration breakpoints of 0.032 and 0.064 mg/L for Candida glabrata and Candida tropicalis, respectively. Additionally, a probability of target attainment of ≥ 90% could not be achieved for Candida krusei or Candida parapsilosis with a 300 mg daily dose.

Conclusions: The recommended daily dose of micafungin (100 mg) may produce low clinical success ratios in non-Candida albicans infections; therefore, higher doses should be administered to improve clinical outcomes.
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http://dx.doi.org/10.1007/s11095-020-02980-zDOI Listing
January 2021

Evaluating influential factors of acupuncture for tension-type headache: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2020 Nov;99(46):e23118

School of Health and Biomedical Sciences, RMIT University, Melbourne, Australia.

Background: Acupuncture has been recommended for the treatment of tension-type headache (TTH). However inconsistent findings on the efficacy of acupuncture impacts on the uptake of this effective therapy for TTH. This systematic review aims to 1) Evaluate the efficacy of acupuncture for TTH; and 2) Clarify the factors contributing to conflicting findings through conducting a meta-analysis and meta-regression of randomized controlled trials.

Methods: Comprehensive literature search will be performed on PubMed, EMBASE, CINAHL, ProQuest, Cochrane Central Register of Controlled Trials, Acubriefs, ScienceDirect, Scopus, AMED, and 4 leading Chinese databases of the China National Knowledge Infrastructure, China Science and Technology Journal Database, Wanfang Data, and CBM (SinoMed). We will include Randomized controlled Trials or controlled trials of patients with TTH that compared acupuncture with sham interventions. The primary outcome is the number of days on headache (within 4 weeks) at the end of the treatment and follow-ups. Secondary outcomes include intensity of pain, frequency of attack, and the adverse effects resulting from the intervention. We will use pre-defined sub-group analysis and meta-regression to explore the influential factors of acupuncture effects. Heterogeneity assessment will be performed before carrying out meta-analysis, whereas the subgroup analyses and meta-regression will be used in verifying the possible factors of heterogeneity when significant heterogeneity detected.

Results: Review Manager 5.3 software will be used for meta-analysis. The synthesis will be performed by generating forest plots. Meta-regression will be used to understand influential factors for acupuncture in patients with TTH.

Conclusion: By utilizing techniques of meta-regression, this study will provide evidence toward to a more focused understanding of influential factors for acupuncture in patients with TTH. This systematic review will provide quality evidence for the optimization of acupuncture therapeutic regimen. It will facilitate the development of clinical practice guideline on acupuncture for TTH.
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http://dx.doi.org/10.1097/MD.0000000000023118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7668429PMC
November 2020

Directly observing intracellular nanoparticle formation with nanocomputed tomography.

Sci Adv 2020 Oct 23;6(43). Epub 2020 Oct 23.

Hefei National Laboratory of Physical Sciences at Microscale, Department of Chemistry, University of Science and Technology of China, 96 Jinzhai Road, Hefei, Anhui 230026, China.

Directly observing intracellular nanostructure formation remains challenging. In this work, using a rationally designed small-molecule 4-nitrobenzyl carbamate-Cys(SEt)-Asp-Asp-Phe(iodine)-2-cyano-benzothiazole (NBC-Iod-CBT), we directly observed intracellular nanoparticle formation with nanocomputed tomography (nano-CT). In vitro, upon glutathione reduction and nitroreductase (NTR) cleavage, NBC-Iod-CBT undergoes a CBT-Cys click condensation reaction to self-assemble nanoparticles Iod-CBT-NPs with an average linear absorption coefficient (LAC) value of 0.182 ± 0.078 μm to x-ray. Nano-CT imaging of the NBC-Iod-CBT-treated, NTR-overexpressing HeLa cells showed the existence of Iod-CBT-NPs in their cytoplasm with an average LAC value of 0.172 ± 0.032 μm We anticipate that our strategy could help people to deeply understand the formation mechanism of intracellular nanostructures in the near future.
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http://dx.doi.org/10.1126/sciadv.aba3190DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7608822PMC
October 2020

Peripheral Blood S100B Levels in Autism Spectrum Disorder: A Systematic Review and Meta-Analysis.

J Autism Dev Disord 2020 Oct 2. Epub 2020 Oct 2.

Department of Pediatrics, The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou, 510630, Guangdong, China.

The S100 calcium-binding protein beta subunit (S100B) protein, which mostly exists in the central nervous system, is commonly noted as a marker of neuronal damage. We conducted the first systematic review with meta-analysis to compare peripheral blood S100B levels in individuals with ASD with those in healthy controls. A systematic search was carried out for studies published before May 5, 2020. In total, this meta-analysis involved ten studies with 822 participants and 451 cases. The meta-analysis revealed that individuals with ASD had higher peripheral blood S100B levels than healthy controls [standardized mean difference (SMD) = 0.97, 95% confidence interval (95% CI) = 0.41-1.53; p < 0.001]. Peripheral blood S100B levels may have potential as a useful biomarker for ASD.
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http://dx.doi.org/10.1007/s10803-020-04710-1DOI Listing
October 2020

Effects of Tai Chi Yunshou on upper limb function and balance in stroke survivors: A protocol for systematic review and meta analysis.

Medicine (Baltimore) 2020 Jul;99(29):e21040

Hospital of Chengdu University of Traditional Chinese Medicine.

Background: Functional disability is the most common disorder that occurs after stroke and seriously affects the quality of life of stroke survivors. Tai Chi Yunshou (TCY), a fundamental form of Tai Chi, is a simple, convenient, and economical exercise therapy from ancient China. Some clinical trials have reported that it improves upper limb function and balance during stroke rehabilitation. Thus, we plan to conduct a systematic review to investigate the effects of TCY in stroke survivors.

Methods: This review will follow the Preferred Reporting Items for Systematic Reviews and Meta-analyses statement. We will search English and Chinese databases for randomized controlled trials on TCY for stroke survivors from the dates when the databases were established to 1 July 2020. The English databases will include MEDLINE (PubMed), EMBASE (embase.com), and the Cochrane Central Register of Controlled Trials (Cochrane Library). In addition, the Chinese databases will include the Chinese National Knowledge Infrastructure, the Chinese Biomedical Literature Database, the Chinese Science and Technology Periodical Database, the Wanfang database, and the Chinese Dissertation Database. The primary outcomes will include upper limb function and balance function, as measured by the Fugl-Meyer assessment and Berg balance scale, respectively. Two reviewers will independently screen the studies on the basis of the inclusion criteria and extract data. Review Manager (v5.3) will be used for data synthesis, and Cochrane Collaboration's tool will be used to assess the risk of bias. A fixed effects model or a random effects model will be selected based on the level of heterogeneity. The grading of recommendations assessment, development, and evaluation system will be used to evaluate the quality of the outcomes.

Results: This systematic review results will be carried out after the completion of the protocol.

Conclusions: This protocol aims to guide a systematic review and meta-analysis investigating the effects of JCY on upper limb function and body balance in stroke survivors, which will provide evidence for post-stroke rehabilitation training.

Prospero Registration Number: CRD42020169549.
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http://dx.doi.org/10.1097/MD.0000000000021040DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7373622PMC
July 2020

Unfractionated heparin attenuates endothelial barrier dysfunction via the phosphatidylinositol-3 kinase/serine/threonine kinase/nuclear factor kappa-B pathway.

Chin Med J (Engl) 2020 Aug;133(15):1815-1823

Department of Intensive Care Unit, Cancer Hospital of China Medical University, Liaoning Cancer Hospital and Institute, Shenyang, Liaoning 110042, China.

Background: Vascular endothelial dysfunction is considered a key pathophysiologic process for the development of acute lung injury. In this study, we aimed at investigating the effects of unfractionated heparin (UFH) on the lipopolysaccharide (LPS)-induced changes of vascular endothelial-cadherin (VE-cadherin) and the potential underlying mechanisms.

Methods: Male C57BL/6 J mice were randomized into three groups: vehicle, LPS, and LPS + UFH groups. Intraperitoneal injection of 30 mg/kg LPS was used to induce sepsis. Mice in the LPS + UFH group received subcutaneous injection of 8 U UFH 0.5 h before LPS injection. The lung tissue of the mice was collected for assessing lung injury by measuring the lung wet/dry (W/D) weight ratio and observing histological changes. Human pulmonary microvascular endothelial cells (HPMECs) were cultured and used to analyze the effects of UFH on LPS- or tumor necrosis factor-alpha (TNF-α)-induced vascular hyperpermeability, membrane expression of VE-cadherin, p120-catenin, and phosphorylated myosin light chain (p-MLC), and F-actin remodeling, and on the LPS-induced activation of the phosphatidylinositol-3 kinase (PI3K)/serine/threonine kinase (Akt)/nuclear factor kappa-B (NF-κB) signaling pathway.

Results: In vivo, UFH pretreatment significantly attenuated LPS-induced pulmonary histopathological changes (neutrophil infiltration and erythrocyte effusion, alveolus pulmonis collapse, and thicker septum), decreased the lung W/D, and increased protein concentration (LPS vs. LPS + UFH: 0.57 ± 0.04 vs. 0.32 ± 0.04 mg/mL, P = 0.0092), total cell count (LPS vs. LPS + UFH: 9.57 ± 1.23 vs. 3.65 ± 0.78 × 10/mL, P = 0.0155), polymorphonuclear neutrophil percentage (LPS vs. LPS + UFH: 88.05% ± 2.88% vs. 22.20% ± 3.92%, P = 0.0002), and TNF-α (460.33 ± 23.48 vs. 189.33 ± 14.19 pg/mL, P = 0.0006) in the bronchoalveolar lavage fluid. In vitro, UFH pre-treatment prevented the LPS-induced decrease in the membrane expression of VE-cadherin (LPS vs. LPS + UFH: 0.368 ± 0.044 vs. 0.716 ± 0.064, P = 0.0114) and p120-catenin (LPS vs. LPS + UFH: 0.208 ± 0.018 vs. 0.924 ± 0.092, P = 0.0016), and the LPS-induced increase in the expression of p-MLC (LPS vs. LPS + UFH: 0.972 ± 0.092 vs. 0.293 ± 0.025, P = 0.0021). Furthermore, UFH attenuated LPS- and TNF-α-induced hyperpermeability of HPMECs (LPS vs. LPS + UFH: 8.90 ± 0.66 vs. 15.84 ± 1.09 Ω·cm, P = 0.0056; TNF-α vs. TNF-α + UFH: 11.28 ± 0.64 vs. 18.15 ± 0.98 Ω·cm, P = 0.0042) and F-actin remodeling (LPS vs. LPS + UFH: 56.25 ± 1.51 vs. 39.70 ± 1.98, P = 0.0027; TNF-α vs. TNF-α + UFH: 55.42 ± 1.42 vs. 36.51 ± 1.20, P = 0.0005) in vitro. Additionally, UFH decreased the phosphorylation of Akt (LPS vs. LPS + UFH: 0.977 ± 0.081 vs. 0.466 ± 0.035, P = 0.0045) and I kappa B Kinase (IKK) (LPS vs. LPS + UFH: 1.023 ± 0.070 vs. 0.578 ± 0.044, P = 0.0060), and the nuclear translocation of NF-κB (LPS vs. LPS + UFH: 1.003 ± 0.077 vs. 0.503 ± 0.065, P = 0.0078) in HPMECs, which was similar to the effect of the PI3K inhibitor, wortmannin.

Conclusions: The protective effect of UFH against LPS-induced pulmonary endothelial barrier dysfunction involves VE-cadherin stabilization and PI3K/Akt/NF-κB signaling.
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http://dx.doi.org/10.1097/CM9.0000000000000905DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7470014PMC
August 2020

Effect of Electroacupuncture on Postoperative Gastrointestinal Recovery in Patients Undergoing Thoracoscopic Surgery: A Feasibility Study.

Med Sci Monit 2020 May 23;26:e920648. Epub 2020 May 23.

Department of Anesthesiology, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China (mainland).

BACKGROUND The aim of this study was to study the feasibility and acceptability of electroacupuncture (EA) for preventing postoperative gastrointestinal complications in patients undergoing thoracoscopic segmentectomy/lobectomy. MATERIAL AND METHODS Sixty patients who underwent video-assisted thoracoscopic (VATS) segmentectomy/lobectomy received either EA treatments plus usual care (EA group) or usual care alone (UC group). Patients in the EA group were given 30 minutes of bilateral electroacupuncture on 3 acupoints [Neiguan (PC6), Zusanli (ST36), and Shangjuxu (ST37)] at 3 time points (24 hours before surgery, and 4 hours and 24 hours after surgery). The primary outcomes were recruitment, retention, acceptability of the EA intervention, incidence and severity of abdominal distension (AD), and time to first flatus and defecation. Secondary outcomes included postoperative nausea and vomiting (PONV), pain intensity, and duration of hospital stay. RESULTS We recruited 60 participants and 59 were randomized into 2 groups for this study: 30 in the EA group and 29 in the UC group. In total, 57 participants completed the study. With the exception of one participant in the EA group, all participants completed all three sessions of EA. The one exclusion was a case where a paravertebral block was not used during the surgery. Qualitative findings from the acceptability questionnaire indicated that participants viewed the EA treatment as acceptable. After EA treatment, there was a small but statistically significant improvement in participants' acceptance of EA for alleviating postoperative gastrointestinal discomfort (P=0.001). The EA group showed improved outcomes compared to the UC group in terms of time to first flatus (20.8±4.6 versus 24.1±6.2 hours, P=0.026) and defecation (53.9±6.0 versus 57.5±7.2 hours, P=0.046). No significant differences appeared regarding AD, rescue medication, or duration of hospitalization. PONV and pain intensity were similar in both groups at the recorded time periods. CONCLUSIONS EA is feasible and acceptable to patients undergoing VATS surgery. Our preliminary findings of EA promoting postoperative recovery of gastrointestinal function warrants large randomized controlled trials.
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http://dx.doi.org/10.12659/MSM.920648DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7260999PMC
May 2020

Bioinspired Hydrophobic Cellulose Nanocrystal Composite Films as Organic-Solvent-Responsive Structural-Color Rewritable Papers.

ACS Appl Mater Interfaces 2020 Jun 28;12(23):26455-26463. Epub 2020 May 28.

School of Chemistry and Chemical Engineering, State Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University, Shanghai 200240, P. R. China.

Lots of beetles, moths, and birds in the natural world present stunning unique structural colors as well as excellent hydrophobic performances. Herein, a novel bioinspired variable structural-color film with organic-solvent responsiveness and surface hydrophobicity was fabricated. Cellulose nanocrystals (CNCs) provided structural color with left-handed helicity. PEG-PPG-PEG triblock copolymers (PPPTCs) were blended with CNCs, giving rise to the organic-solvent-responsive structural color and wider red-shift window of the reflectance peak. The color of the film could be regulated repeatedly under the stimulus of cyclohexanone with an obvious red shift up to 107 nm, corresponding to a macroscopic color change from blue to yellow. Low-surface-energy compound hexadecyltrimethoxysilane (HDTMS) was covalently grafted on the surface in a one-step method to introduce hydrophobicity, successfully preventing the effect of water on the ordered nanostructure. Based on the bionics principle, the as-prepared CNC/PPPTC nanocomposite films with variable structural colors and hydrophobicity are beneficial to their prospective applications in display screens, rewritable hydrophobic structural-color-changing paper, biomimetic sensors, and so forth.
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http://dx.doi.org/10.1021/acsami.0c04785DOI Listing
June 2020

Verum- versus Sham-Acupuncture on Alzheimer's Disease (AD) in Animal Models: A Preclinical Systematic Review and Meta-Analysis.

Biomed Res Int 2020 31;2020:5901573. Epub 2020 Mar 31.

Human Nutrition Department, College of Health Science, QU Health, Qatar University, Doha, Qatar.

Background: Alzheimer's disease (AD) is a common health condition affecting senile people and leads to severe cognitive dysfunctions. Acupuncture has been shown to be a possible alternative natural remedy for AD in some animal studies.

Objective: To perform a systematic review to identify the effect of verum-acupuncture compared with sham-acupuncture on learning and memory performance among animal models of AD.

Methods: Experimental animal studies of treating AD via verum- and sham- acupuncture were searched in nine electronic databases, including Sciverse ScienceDirect, PubMed, Springer, Ebsco Medline, AMED, EMBASE (Elsevier), Scopus (Elsevier), PsycINFO (ProQuest), and OVID from the dates of the databases' inception to June 2019. The Morris water maze test was considered as an outcome measure. The software Revman 5.3 and Stata 16.0 were used to conduct the meta-analysis. Heterogeneity was examined by using I statistics. The publication bias was assessed via Begg's test by Stata 16.0.

Results: Twelve studies involving 229 animals met the inclusion criteria. Most of the studies had a moderate quality according to SYRCLE's risk of bias tool for animal studies. The results of the meta-analysis indicated that verum-acupuncture could reduce the escape latency (MD = -12.90, 95% CI (-17.08, -8.71), < 0.001) and increase the time spent in the original platform quadrant (MD = 7.28, 95% CI (4.23, 10.33), < 0.001) and frequency of the crossing former platform (MD = 2.01, 95% CI (1.53, 2.50), < 0.001) compared with the sham-acupuncture.

Conclusions: Acupuncture is effective in improving cognitive functions in AD animal models, and this benefit is more than just a placebo effect. Further clinical trials are needed to confirm the findings.
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http://dx.doi.org/10.1155/2020/5901573DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7150729PMC
January 2021

Topological supersolidity of dipolar Fermi gases in a spin-dependent optical lattice.

J Phys Condens Matter 2020 May;32(23):235701

Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, People's Republic of China. School of Physical Sciences, University of Chinese Academy of Sciences, Beijing100190, People's Republic of China.

We investigate the topological supersolid states of dipolar Fermi gases trapped in a spin-dependent 2D optical lattice. Our results show that topological supersolid states can be achieved via the combination of topological superfluid states with the stripe order. Different from the general held belief that supersolid state in fermionic system can only survive with simultaneous coexistence of the repulsive and attractive dipolar interaction. We demonstrate that it can be maintained when the dipolar interaction is attractive in both x and y direction. By adjusting the ratio of hopping amplitude between different directions and dipolar interaction strength U, the system will undergo a phase transition among p + ip superfluid state, p -wave superfluid state, and the topological supersolid state. The supersolid state in the attractive environment is proved to be stable by the positive sign of the inverse compressibility. We also design an experimental protocol to realize the staggered next-next-nearest-neighbor hopping via the laser assisted tunneling technique, which is the key to simulate the spin-dependent potential.
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http://dx.doi.org/10.1088/1361-648X/ab7871DOI Listing
May 2020

Experimental demonstration of a graphene-based hybrid plasmonic modulator: publisher's note.

Opt Lett 2020 Feb;45(4):827

This publisher's note contains corrections to Opt. Lett.44, 2586 (2019)OPLEDP0146-959210.1364/OL.44.002586.
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http://dx.doi.org/10.1364/OL.389201DOI Listing
February 2020

Cofilin-actin rod formation in experimental stroke is attenuated by therapeutic hypothermia and overexpression of the inducible 70 kD inducible heat shock protein (Hsp70).

Brain Circ 2019 Oct-Dec;5(4):225-233. Epub 2019 Dec 27.

University of California, San Francisco and the San Francisco Veterans Affairs Medical Center, San Francisco, CA, USA.

Background And Purpose: Cofilin-actin rods are covalently linked aggregates of cofilin-1 and actin. Under ischemic conditions, these rods have been observed in neuronal dendrites and axons and may contribute to the loss of these processes. Hypothermia (Hypo) and the 70 kD inducible heat shock protein (Hsp70) are both known to improve outcomes after stroke, but the mechanisms are uncertain. Here, we evaluated the effect of these factors on cofilin-actin rod formation in a mouse model of stroke.

Materials And Methods: Mice were subjected to distal middle cerebral artery occlusion (dMCAO) and treated with Hypo using a paradigm previously shown to be neuroprotective. We similarly studied mice that overexpressed transgenic (Tg) or were deficient knockout (Ko) in the inducible 70 kDa heat shock protein (Hsp70), also previously shown to be protective by our group and others. Cofilin-actin rod formation was assessed by histological analysis at 4 and 24 h after dMCAO. Its expression was analyzed in three different regions, namely, infarct core (the center of the infarct), middle cerebral artery (MCA) borderzone (the edge of the brain regions supplied by the MCA), and the ischemic borderzone (border of ischemic lesion). Ischemic lesion size and neurological deficits were also assessed.

Results: Both Hypo-treated and Hsp70 Tg mice had smaller lesion sizes and improved neurological outcomes, whereas Hsp70 Ko mice had larger lesion sizes and worsened neurological outcomes. Cofilin-actin rods were increased after stroke, but were reduced by therapeutic Hypo and in Hsp70 Tg mice. In contrast, cofilin-actin rods were increased in ischemic brains of Hsp70 Ko mice.

Conclusions: Cofilin-actin rod formation was suppressed under the conditions of neuroprotection and increased under circumstances where outcome was worsened. This suggests that cofilin-actin rods may act to participate in or exacerbate ischemic pathology and warrants further study as a potential therapeutic target.
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http://dx.doi.org/10.4103/bc.bc_52_19DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6950512PMC
December 2019

Polysaccharide-based recoverable double-network hydrogel with high strength and self-healing properties.

J Mater Chem B 2020 01 6;8(4):794-802. Epub 2020 Jan 6.

School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.

Polysaccharide-based hydrogels (PSBHs) have received significant attention for numerous bio-applications due to their biocompatibility and non-immunogenic performance. However, the construction of PSBH with superior mechanical properties by a simple method is rarely adequately researched. This study focuses on the construction of a novel PSBH with superior mechanical and recoverable properties by integrating the synergistic and complementary interactions of covalent bond-associated oxidized sodium alginate (SA-CHO) gel and hydrogen bond-associated agarose (Aga) gel. With the synergy and complementarity of the SA-CHO and Aga networks, the hydrogel exhibited 17 and 15 times (20 and 9 times) greater compressive stress and modulus, respectively, compared with the SA-CHO gel (Aga gel). The hydrogel also displayed excellent fatigue resistance, recurrent shapeability, acid resistance and recovery ability, as well as self-healing ability. This study provides a unique perspective for enhancing the mechanical properties of PSBH through the synergy and complementarity of different kinds of polysaccharides without sacrificing the functionality of the PSBH.
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http://dx.doi.org/10.1039/c9tb01679aDOI Listing
January 2020

Using patient self-checklist to improve the documentation of risk of postoperative nausea and vomiting: an implementation project.

Int J Evid Based Healthc 2020 Mar;18(1):65-74

School of Health and Biomedical Sciences, RMIT University, Bundoora, Victoria, Australia.

Background And Aims: Postoperative nausea and vomiting (PONV) is a common surgical complication, affecting 30-50% of patients and 80% in high risk populations. Successful prevention and management of PONV relies on accurately assessing individual risk prior to surgery. A valid and reliable Apfel score is commonly used to assess patients' risk. It is however challenging to translate this evidence into clinical practice. This evidence-based project aimed to identify the current practice of assessing and documenting the risk factors of PONV prior to surgery, and to develop strategies to improve the practice.

Methods: The project had three phrases, including forming a team and conducting the baseline audit; identifying problems and developing strategies; and conducting a follow-up tool to assess the impact on compliance with best practice. A research team was formed. A baseline audit was conducted at a public hospital in Victoria in June 2016 to examine PONV risk assessment practice through checking medical files of surgical patients. A getting research into practice audit and feedback tool was used to identify barriers, implementation strategies, stakeholders and resources. After implementation, a second audit was conducted between June and October 2017. Audit criteria were based on a reliable and valid Apfel score.

Results: At baseline, accurate PONV risk could only be calculated from 8% of patient files with no file formally recording the risk factors. The proportion of patients with three risk factors preoperatively, indicating high PONV risk, was 5.3%. Barriers identified were the perceived lack of necessity to record the risk, time constraint and too much paperwork. A self-checklist for risk assessment was developed to enable patients to check their own level of risk. Its face validity, construct validity and accuracy were examined. The checklist was then implemented for patients to complete prior to surgery. A number of strategies were used to improve the implementation. The second audit of 1308 files showed that at the end of audit period, 74% of patients had risk assessment conducted and documented postimplementation. 16.8% of the patients were identified as having high PONV risk, nearly triple the number identified at baseline.

Conclusion: A simple self-checklist of PONV risk was implemented. It greatly improved PONV risk assessment and documentation in a public hospital in Australia and enabled the identification of patients at high risk.
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http://dx.doi.org/10.1097/XEB.0000000000000213DOI Listing
March 2020

Carboxylesterase-Cleavable Biotinylated Nanoparticle for Tumor-Dual Targeted Imaging.

Theranostics 2019 25;9(24):7359-7369. Epub 2019 Sep 25.

Hefei National Laboratory of Physical Sciences at Microscale, Department of Chemistry, University of Science and Technology of China, 96 Jinzhai Road, Hefei, Anhui 230026, China.

Near-infrared (NIR) nanoprobes with fluorescence "Turn-On" property are advantageous in cancer diagnosis but, to the best of our knowledge, "smart" nanoprobe that simultaneously targets both biotin receptor and carboxylesterase (CES) for HepG2 tumor-dual targeted imaging has not been reported. : Using CBT-Cys click condensation reaction, we rationally designed a "smart" NIR fluorescence probe HN-Cys(StBu)-Lys(Biotin)-Ser(Cy5.5)-CBT () and used it to facilely prepare the fluorescence-quenched nanoparticle . : results indicated that, after was incubated with CES for 6 h, its fluorescence was turned "On" by 69 folds. Cell experiments verified that was uptaken by HepG2 cells biotin receptor-assisted endocytosis and its fluorescence was turned "On" by intracellular CES hydrolysis. Moreover, was successfully applied to image both biotin receptor- and CES-overexpressing HepG2 tumors. : Fluorescence-quenched nanoparticle was facilely prepared to actively target biotin receptor-overexpressing HepG2 cancer cells and turn the fluorescence "On" by intracellular CES hydrolysis for tumor-dual targeted imaging. We anticipate that our fluorescence "Turn-On" nanoparticle could be applied for liver cancer diagnosis in clinic in the near future.
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http://dx.doi.org/10.7150/thno.37625DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6831296PMC
September 2020

Does Electroacupuncture Have Different Effects on Peripheral and Central Sensitization in Humans: A Randomized Controlled Study.

Front Integr Neurosci 2019 15;13:61. Epub 2019 Oct 15.

School of Health and Biomedical Sciences, RMIT University, Melbourne, VIC, Australia.

Background: Acupuncture is used to reduce chronic musculoskeletal pain. The common mechanism underlying these types of pain are peripheral and/or central sensitization. In the clinical setting, it is difficult to separate the peripheral from the central component of pain. Heat/capsaicin 45°C/0.075%-induced hyperalgesia provides a stable, human central sensitization model in which the peripheral component is also assessed.

Aim: This randomized, sham-controlled study aimed to investigate the effect of electroacupuncture (EA) on the severity of heat (peripheral sensitization) and mechanical hyperalgesia (central sensitization) in a heat/capsaicin pain model in humans.

Methods: Twenty-six healthy young participants (24 ± 3.9 years) were recruited. After baseline assessment, heat/capsaicin 45°C/0.075% was applied to the non-dominant forearm to induce hyperalgesia. The primary outcome measures were the size of the area of mechanical hyperalgesia, intensity of pain to heat stimulation and heat pain thresholds. The intensity of pain was recorded using modified 10-cm visual analogues scales (VAS). Participants were assessed at 70 min after the initial application of capsaicin then randomly allocated to receive either real electroacupuncture (REA, = 14) or sham non-invasive EA (SEA, = 12) for 30 min. The main outcome measures were assessed again immediately and then 90 min following EA. Credibility of blinding was assessed. Data were analyzed with -tests or analysis of variance (ANOVA) where appropriate.

Results: After the model was established, the area of mechanical hyperalgesia was formed (55.64 cm), as was heat hyperalgesia, as the rating to heat stimulation, increased from 2/10 to 6/10. The REA and SEA groups were comparable. Immediately after the allocated acupuncture treatment, the rating to heat stimulation was statistically significantly lower in the REA group (2.94 ± 1.64) than in the SEA group (4.62 ± 2.26) ( < 0.05). The area of mechanical hyperalgesia reduced significantly without any group difference. No group difference was detected in heat pain threshold. Blinding of the participants was successful.

Conclusion: Peripheral and central sensitization in the heat/capsaicin 45°C/0.075% model responded to EA differently, suggesting that acupuncture analgesia could vary, depending on the types of pain. This observation may explain some inconsistent findings from clinical trials of acupuncture.
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http://dx.doi.org/10.3389/fnint.2019.00061DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6804574PMC
October 2019

Nanosecond photochemically promoted click chemistry for enhanced neuropeptide visualization and rapid protein labeling.

Nat Commun 2019 10 16;10(1):4697. Epub 2019 Oct 16.

School of Pharmacy, University of Wisconsin-Madison, Madison, WI, 53705, USA.

Comprehensive protein identification and concomitant structural probing of proteins are of great biological significance. However, this is challenging to accomplish simultaneously in one confined space. Here, we develop a nanosecond photochemical reaction (nsPCR)-based click chemistry, capable of structural probing of proteins and enhancing their identifications through on-demand removal of surrounding matrices within nanoseconds. The nsPCR is initiated using a photoactive compound, 2-nitrobenzaldehyde (NBA), and is examined by matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS). Benefiting from the on-demand matrix-removal effect, this nsPCR strategy enables enhanced neuropeptide identification and visualization from complex tissue samples such as mouse brain tissue. The design shows great promise for structural probing of proteins up to 155 kDa due to the exclusive accessibility of nsPCR to primary amine groups, as demonstrated by its general applicability using a series of proteins with various lysine residues from multiple sample sources, with accumulated labeling efficiencies greater than 90%.
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http://dx.doi.org/10.1038/s41467-019-12548-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6795811PMC
October 2019

In Vitro and in Vivo Characterization of a Foam-Like Polyurethane Bone Adhesive for Promoting Bone Tissue Growth.

ACS Biomater Sci Eng 2019 Oct 30;5(10):5489-5497. Epub 2019 Aug 30.

School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.

Biocompatible bone adhesive is a kind of promising biomaterial in orthopedic and trauma surgery. However, despite the urgent need, there is no appropriate system that can meet all the requirements for bone adhesive. In this study, a porous polyurethane adhesive (PUA) was fabricated, and the effects of water, polyisocyanate, and β-tricalcium phosphate (β-TCP) on the physicochemical and mechanical properties of PUAs were investigated. By varying the water content, PUAs with different surface morphologies and porosities were prepared. The adhesion strength and mechanical property of the adhesives could be enhanced by the addition of polyisocyanate and β-TCP. The adhesion strength of the PUA to bone was 2 times higher than that of clinical poly(methyl methacrylate) bone cement. The in vitro cell culture and attachment assays indicated good biocompatibility of the PUAs. In vivo analysis in a rabbit model demonstrated that the porous structure of the PUA could facilitate the growth of cells and bone tissues.
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http://dx.doi.org/10.1021/acsbiomaterials.9b00918DOI Listing
October 2019

Humidity and Heat Dual Response Cellulose Nanocrystals/Poly(-Isopropylacrylamide) Composite Films with Cyclic Performance.

ACS Appl Mater Interfaces 2019 Oct 14;11(42):39192-39200. Epub 2019 Oct 14.

School of Chemistry and Chemical Engineering, State Key Laboratory of Metal Matrix Composites , Shanghai Jiao Tong University , Dongchuan Road No. 800 , Shanghai 200240 , China.

It has been widely reported that cellulose nanocrystals (CNCs) demonstrate a special structural color, which stems from chiral nematic domains. Herein, the humidity and heat dual response nanocomposite films with multilayered helical structure were prepared by self-assembling of CNCs and hydrazone groups modified poly(-isopropylacrylamide) (PNIPAM) copolymers. Furthermore, glutaraldehyde was involved to act as a chemical linker to improve cyclic stability by forming acylhydrazone bonds. The structural color of the films could be easily regulated by humidity, heat, or the content of modified PNIPAM copolymers. The absorption of water in higher humidity led to volume expansion of the resin, resulting in a red shift for up to 145 nm. In contrast, the resin shrank under the temperature above the lower critical solution temperature of PNIPAM, leading to a blue shift for up to 87 nm. It was notable that the change of color can be easily captured by the naked eyes. Moreover, the films exhibited excellent stability and cyclicity in response to either vapor or liquid water due to the chemical linking between CNCs and resins. The as-prepared CNCs/PNIPAM nanocomposite films with humidity or heat responsibilities are promising in stimuli-responsive sensors, printing industry, surface decorations, and so forth.
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http://dx.doi.org/10.1021/acsami.9b14201DOI Listing
October 2019

Small-molecule inhibitor targeting the Hsp90-Cdc37 protein-protein interaction in colorectal cancer.

Sci Adv 2019 09 18;5(9):eaax2277. Epub 2019 Sep 18.

State Key Laboratory of Natural Medicines and Jiang Su Key Laboratory of Drug Design and Optimization, China Pharmaceutical University, Nanjing 210009, China.

Disrupting the interactions between Hsp90 and Cdc37 is emerging as an alternative and specific way to regulate the Hsp90 chaperone cycle in a manner not involving adenosine triphosphatase inhibition. Here, we identified DDO-5936 as a small-molecule inhibitor of the Hsp90-Cdc37 protein-protein interaction (PPI) in colorectal cancer. DDO-5936 disrupted the Hsp90-Cdc37 PPI both in vitro and in vivo via binding to a previously unknown site on Hsp90 involving Glu, one of the binding determinants for the Hsp90-Cdc37 PPI, leading to selective down-regulation of Hsp90 kinase clients in HCT116 cells. In addition, inhibition of Hsp90-Cdc37 complex formation by DDO-5936 resulted in a remarkable cyclin-dependent kinase 4 decrease and consequent inhibition of cell proliferation through Cdc37-dependent cell cycle arrest. Together, our results demonstrated DDO-5936 as an identified specific small-molecule inhibitor of the Hsp90-Cdc37 PPI that could be used to comprehensively investigate alternative approaches targeting Hsp90 chaperone cycles for cancer therapy.
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http://dx.doi.org/10.1126/sciadv.aax2277DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6750927PMC
September 2019

Interlock or Chemical Bond: Investigation on the Interface of Graphene Oxide and Styrenic Block Copolymers as Layer-by-Layer Films.

ACS Omega 2019 May 23;4(5):9120-9128. Epub 2019 May 23.

School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.

In the paper, graphene oxide (GO) and two kinds of styrenic resins, poly[styrene--(ethylene--butylene)--styrene] (SEBS) and maleic anhydride (MA) grafted SEBS (MA--SEBS), were utilized to explore the interfacial interaction of carbon-based materials and block copolymers as layer-by-layer (LBL) assembly films. The details of the interlayer interaction of the two kinds of composite films were investigated through the analysis of the mechanical properties and internal structure of the composites. For the SEBS/GO composite film, the "interlock" structure tended to form between the GO sheets and SEBS resin, and the physical "interlocking effect" could make full use of the excellent mechanical properties of GO nanosheets. As a result, both failure strength and elongation at break of the SEBS/GO composite film were enhanced by 50 and 25%, respectively. On the other hand, some different structures were found in the MA--SEBS/GO composite film, where the GO sheets stacked onto the resin closely because of the chemical interaction between them and no obvious "interlocks" was found within the interface, and the chemical interface interaction was strong enough to prevent the slide of GO nanosheets under tension after the graphene sheets were highly oxidized, so the mechanical properties of the MA--SEBS/GO composite film could be also enhanced. Based on an overall consideration of the research results of these LBL assembled composites, choosing more perfect materials and structures is needed, which should use physical and chemical interfacial interactions more efficiently, to obtain better mechanical properties of inorganic carbon-organic resin composites.
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http://dx.doi.org/10.1021/acsomega.9b00515DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6648628PMC
May 2019