Publications by authors named "Zhen Zhang"

2,695 Publications

  • Page 1 of 1

Metabolic responses of Chinese perch (Siniperca chuatsi) to different levels of dietary carbohydrate.

Fish Physiol Biochem 2021 Jul 29. Epub 2021 Jul 29.

College of Fisheries, Chinese Perch Research Center, Huazhong Agricultural University, No. 1, Shizishan Street, Hongshan District, Wuhan, 430070, Hubei Province, China.

There are great differences in metabolic responses to different levels of carbohydrate among different carnivorous fish species. To explore metabolic responses of Chinese perch to moderate and high level of dietary carbohydrates, three diets containing 7.3% (LC), 17.5% (MC), and 27.5% (HC) of carbohydrates were provided to Chinese perch for 56 days. The results showed that MC and HC groups exhibited an increase in weight gain (WG) and hepatic glycogen content, and a decrease in feed conversion efficiency, compared with the LC group. The MC and HC groups also showed the increase in mRNA levels of phosphofructokinase and citrate synthase related to the aerobic oxidation pathway, which might be responsible for the increase in WG. Moreover, compared with the LC group, the HC group exhibited high levels of plasma indices (glucose, pyruvic acid, lactic acid, total triglyceride, total cholesterol, and low-density lipoprotein) and liver lipid resulting from the increased mRNA levels of fatty acid synthesis-related genes (ATP citrate lyase, acetyl-CoA carboxylase α, and fatty acid synthase), low level of crude protein caused by inhibition of TOR pathway, and liver damage induced by low antioxidant capacity and infiltration of inflammatory cells, but the MC group did not. The above results indicated that 17.5% dietary carbohydrate might be utilized effectively in Chinese perch and part carbohydrates were converted into glycogen to maintain glucose homeostasis; 27.5% dietary carbohydrate could not be fully utilized. The 27.5% carbohydrate diet induced the up-regulation of aerobic oxidation, glycogen synthesis, and fat synthesis pathways which might not be sufficient to maintain glucose homeostasis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10695-021-00965-2DOI Listing
July 2021

Ecological rice-cropping systems mitigate global warming - A meta-analysis.

Sci Total Environ 2021 May 24;789:147900. Epub 2021 May 24.

College of Resources and Environment, Hunan Agricultural University, Changsha 410125, China. Electronic address:

Ecological rice-cropping systems (ERSs) are prosperous rice ecosystems that have a profound influence on global greenhouse (GHG) effects. However, the high variation in research results requires an accurate evaluation of the ERS effects. In this study, three typical ERS modes, rice-crayfish, rice-duck, and rice-fish were selected, and a meta-analysis was conducted using the data of 34 studies to comprehensively evaluate the effects of ERSs on GHG emissions, the global warming potential (GWP), and GHG intensity (GHGI). The results showed that the ERSs reduced CH4 emissions significantly (-12.5%), but increased N2O emissions by 11.3% as compared with traditional rice-cropping systems (TRSs). Further, ERSs have slightly lower GWP, rice yield, and GHGI values (6.5%, 5.5%, and 5.6%, respectively) than TRSs. The rice-crayfish and rice-duck modes significantly alleviated the GWP by 18.0% and 11.1%, respectively, whereas the rice-fish mode enhanced the GWP by 20.8%. Moreover, the rice-duck mode significantly reduced the GHGI by 17.2%, while the ricecrayfish and rice-fish modes increased the GHGI by 9.7% and 8.8%, respectively. Further, the ERSs significantly changed the dissolved oxygen concentration in the flood water as well as the Eh, dissolved organic carbon, and ammonium nitrogen in the soil, wherein the effect sizes of the ERSs on the GHG emissions were significantly correlated with their respective increase. Considering the net ecosystem economic budget and CO2 emissions equivalent/output, ERSs were found to be effective "green technologies". Further, we found that the rice-duck ERS was a good ecological ricecropping system for global warming mitigation. Our study provided new ideas for sustainable agriculture.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2021.147900DOI Listing
May 2021

Barren environment damages cognitive abilities in fish: Behavioral and transcriptome mechanisms.

Sci Total Environ 2021 Jul 1;794:148805. Epub 2021 Jul 1.

Fisheries College, Zhejiang Ocean University, Zhoushan 316022, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China. Electronic address:

The surrounding environments that animals inhabit shape their behavioral phenotypes, physiological status and molecular processes. As one of the driving forces for the adaptation and evolution of marine animals, environmental complexity has been shown to affect several behavioral characteristics in fish. However, little is known about the effects of environmental complexity on fish spatial cognition and about the relevant regulatory mechanisms. To address this theoretical gap, black rockfish Sebastes schlegelii, which is a typical rock fish species, were exposed to laboratory-based small-scale contrasting environments (i.e., spatially complex environment vs. spatially barren environment) for seven weeks. Subsequently, the spatial cognitive abilities and behavioral performance during captive period were determined, and transcriptome sequencing and analyses for fish telencephalon were conducted. In general, the fish from barren environment had significantly lower spatial learning and memory abilities compared with the fish from complex environment (i.e., the complex fish exited the maze faster). During the whole captive period, the frequency of aggressive behavior among barren fish was significantly higher than complex fish. And meanwhile, the group dispersion index of barren group was also significantly higher than complex group, which indicated that complex fish tended to distribute in a more homogeneous pattern than barren fish. Through transcriptomic analyses, a series of differentially expressed genes and pathways which may underpin the damaged effects of barren environment on fish spatial cognition were identified, and these genes mainly related to stress response, metabolism, organism systems and neural plasticity. However, no significant differences in growth performance, locomotor activity (indicated by swimming behavior and rotatory behavior) between treatments were detected. Based on these results, mechanisms in the levels of behavior and molecule were proposed to explain the environmental effects on fish cognition. This study may provide fundamental information for deeply understanding the environmental effects on marine animals.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2021.148805DOI Listing
July 2021

Update in version 2021 of CSCO guidelines for colorectal cancer from version 2020.

Chin J Cancer Res 2021 Jun;33(3):302-307

Department of Medical Oncology, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.21147/j.issn.1000-9604.2021.03.02DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8286893PMC
June 2021

Assessment of EGFP-Q74 degradation for the measurement of autophagic flux.

Methods Cell Biol 2021 27;165:31-38. Epub 2021 Apr 27.

Centre de Recherche des Cordeliers, Équipe 11 Labellisée par la Ligue Contre le Cancer, Université de Paris, Sorbonne Université, Inserm U1138, Institut Universitaire de France, Paris, France; Metabolomics and Cell Biology Platforms, Gustave Roussy Cancer Campus, Villejuif, France. Electronic address:

Autophagy plays a major role in physiological and pathological processes. The quantitation of the abundance of autophagy-specific substrates constitutes an efficient strategy for assessing autophagic activity. Here, we provide a detailed protocol for quantifying the decay of a fusion protein composed by enhanced green fluorescent protein (EGFP) and glutamine repeats (Q74) using regular or high-throughput fluorescence microscopy. This method provides a direct measurement of autophagic flux in a Huntington's disease model.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/bs.mcb.2020.10.018DOI Listing
April 2021

Rapidly Thermoreversible and Biodegradable Polypeptide Hydrogels with Sol-Gel-Sol Transition Dependent on Subtle Manipulation of Side Groups.

Biomacromolecules 2021 Jul 23. Epub 2021 Jul 23.

CAS Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, P. R. China.

Thermoreversible hydrogels are attractive materials for biomedical applications, but their applications are still limited by nonbiodegradability and/or slow temperature-dependent gel-to-sol transition rates. In this research, we prepared a range of amphiphilic diblock, triblock, and four-armed star block copolymers composed of poly(ethylene glycol) (PEG) and poly(γ-(2-(2-ethoxyethoxy)ethyl)-l-glutamate) (P(EEOLG)) segments, which can form rapidly thermoreversible hydrogels at physiological temperature. Intriguingly, the obtained hydrogels can transform from gel to sol within 10-70 s in response to the temperature decrease from 37 to 0 °C. The thermosensitive sol-gel-sol transitions are markedly faster than previously reported thermoreversible PEG-poly(l-glutamate) derivative hydrogels with subtle differences in the side groups and a widely studied poly(d,l-lactide--glycolide)--PEG--poly(d,l-lactide--glycolide) (PLGA-PEG-PLGA) hydrogel that required a much longer time of 40∼150 min. Further investigation of the relationship between the hydrogel property and polymer structure is performed, and the self-assembly mechanisms of different copolymers are proposed. Cytotoxicity assays and subcutaneous degradation experiments reveal that the PEG/P(EEOLG) block copolymers are biocompatible and biodegradable. The polypeptide hydrogel can therefore be used as a three-dimensional platform for facile cell culture and collection by regulating the temperature.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.biomac.1c00583DOI Listing
July 2021

Localized Chemotherapy Based on Injectable Hydrogel Boosts the Antitumor Activity of Adoptively Transferred T Lymphocytes In Vivo.

Adv Healthc Mater 2021 Jul 23:e2100814. Epub 2021 Jul 23.

Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun, 130022, P. R. China.

The adoptive transfer of antigen-specific T cells has been successfully applied in the treatment of hematological malignancies. However, its application in the treatment of solid tumors has been overshadowed by the immunosuppressive tumor microenvironment. In this context, a preprocessing strategy is developed to reprogram the immunosuppressive tumor microenvironment using a thermoresponsive hydrogel loaded with doxorubicin ([email protected]). Compared with hydrogel-based chemotherapy alone or adoptive T cell therapy alone, this combination exhibits enhanced anti-tumor efficacy. In addition to the direct killing of tumor cells, the local chemotherapy releases tumor-associated antigens which enhance the proliferation and effector function of endogenous and adoptively transferred T cells. Moreover, [email protected] significantly reduces the numbers of both myeloid derived suppressor cells and Tregs in tumor microenvironment. It is suggested that [email protected] promotes the efficacy of adoptively transferred T cells against solid tumors, overcoming the key limitations of adoptive T cell therapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/adhm.202100814DOI Listing
July 2021

Factors associated with persistence of early-onset atopic dermatitis up to the age of 12 years: a prospective cohort study in China.

Eur J Dermatol 2021 Jun;31(3):403-408

Department of Dermatology, Xinhua Hospital,, Institute of Dermatology, Shanghai Jiaotong University School of Medicine, Shanghai, China.

Atopic dermatitis (AD) can remit as age increases. However, long-term follow-up studies evaluating disease evolution and related factors of persistence of early-onset AD are sparse. This study aimed to identify factors associated with the persistence of early-onset AD. In this prospective cohort study, 260 patients with onset of AD before age two years old were enrolled. Clinical examination was performed and a questionnaire survey completed at enrolment. In addition, the filaggrin gene (FLG) of all participants was sequenced to identify mutations within this gene. Patients were followed at age six and 12. The remission rate was 50.8% at age six and 70.3% at age 12. Persistent AD was associated with a higher SCORAD index at baseline (p < 0.001), a family history of asthma (p = 0.003) and food allergen sensitization (p = 0.033). However, the presence or absence of FLG mutation did not show any significant association with persistent AD. Prognostic factors for persistence of AD were analysed by logistic regression analysis. Disease severity according to SCORAD index at baseline (OR: 1.039; 95% CI: 1.018-1.059; p < 0.001) and family history of asthma (OR: 3.008; 95% CI: 1.297-7.007; p = 0.011) were risk factors that may predict persistent AD based on multivariate regression analysis. It is important to stratify early-onset AD according to severity and investigate family allergic history in order to establish appropriate individual management. Moreover, genetic factors other than FLG mutation may play more important roles in persistent early-onset AD.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1684/ejd.2021.4045DOI Listing
June 2021

Advances in Biological Application of and Research on Low-Frequency Ultrasound.

Ultrasound Med Biol 2021 Jul 22. Epub 2021 Jul 22.

Department of Ultrasound, First Affiliated Hospital of China Medical University, Shenyang City, Liaoning Province, China. Electronic address:

In recent years, the in-depth study of low-frequency sonophoresis (LFS) has greatly elucidated its biological effects in various therapeutic applications, including drug delivery, enhanced healing, thrombolytic technology, anti-inflammatory effects and tumor treatment. Specifically, numerous studies have reported its use in drug delivery and synergistic antitumor activity, indicating a new treatment direction for cancer. However, there are significant gaps in the understanding of LFS in terms of frequency and sound intensity safety; these issues are becoming increasingly important in understanding the biological effects of LFS ultrasound. This article reviews the treatment mechanism and current applications of LFS technology and discusses and summarizes its safety and application prospects.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ultrasmedbio.2021.06.005DOI Listing
July 2021

Reconstruction of the Evolutionary Biogeography Reveal the origins and diversification of Oysters (Bivalvia: Ostreidae).

Mol Phylogenet Evol 2021 Jul 21:107268. Epub 2021 Jul 21.

Department of Marine Organism Taxonomy and Phylogeny, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, Shandong 266071, China; Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, Shandong 266071, China. Electronic address:

Oysters (Bivalvia: Ostreidae Rafinesque, 1815) live in the intertidal and shallow subtidal areas worldwide. Despite their long evolutionary histories, abundant fossil records, global distribution, and ecological significance, a systematic time-dependent biogeographical analysis of this family is still lacking. Using combined mitochondrial (COI and 16S rRNA) and nuclear (18S rRNA, 28S rRNA, H3 and ITS2) gene makers for 80% (70/88) of the recognized extant Ostreidae, we reconstructed the global phylogenetic and biogeographical relationships throughout the evolutionary history of oysters. The result provided a holistic view of the origin, migration and dispersal patterns of Ostreidae. The phylogenetic results and fossil evidence indicated that Ostreidae originated from the circum-Arctic region in the Early Jurassic. The widening of the Atlantic Ocean and changes in the Tethys Ocean further facilitated their subsequent diversification during the Cretaceous and the Palaeogene periods. In particular, Crassostrea and Saccostrea exhibited relatively low dispersal abilities and their major diversifications were consistent with the tectonic events. Environmental adaptations and reproductive patterns, therefore, should play key roles in the formation of oyster distribution patterners, rather than the dispersal ability of their planktonic larvae. The diversity dynamics inferred by standard phylogenetic are consistent with the fossil record, however, further systematic classification, especially for fossil genus Ostrea, would enhance our understanding on extant and fossil oysters. The present study of the historical biogeography of oysters provides new insights into the evolution and speciation of oysters. Our findings also provide a foundation for the assessment of evolutionary patterns and ecological processes in intertidal and inshore life.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ympev.2021.107268DOI Listing
July 2021

γ-Linolenic Acid Prevents Lipid Metabolism Disorder in Palmitic Acid-Treated Alpha Mouse Liver-12 Cells by Balancing Autophagy and Apoptosis via the LKB1-AMPK-mTOR Pathway.

J Agric Food Chem 2021 Jul 19;69(29):8257-8267. Epub 2021 Jul 19.

Institute of Goats and Sheep Science, Nanjing Agricultural University, No. 1 Weigang, Nanjing, Jiangsu 210095, PR China.

Excessive fat deposition is the main character in nonalcoholic fatty liver disease (NAFLD), while γ-linolenic acid (GLA) is a polyunsaturated fatty acid that can reduce lipid deposition. This study investigated the effect and regulatory mechanism of GLA (100 μM) on lipid metabolism in alpha mouse liver 12 (AML-12) cells treated by 400 μM palmitic acid (PA). GLA reduced lipid content and increased fatty acid β oxidation, as indicated by decreasing triglyceride and cholesterol contents and increasing mRNA and protein expressions of CPT1α and PPARα. GLA relieved oxidative stress caused by PA, upregulated mRNA levels of superoxide dismutase and glutathione peroxidase, and decreased reactive oxygen species content. GLA reduced apoptosis, as indicated by decreases in the BAX/BCL2 expression level and apoptosis percentage. GLA activated autophagy, autophagosome-lysosome fusion, and LKB1-AMPK-mTOR signaling and upregulated mRNA and protein expressions of Beclin-1, autophagy-related 5, and liver kinase B1 (LKB1). These effects of GLA on lipid metabolism disorders of PA-treated hepatocytes were reversed by autophagy inhibitor 3MA and AMPK inhibitor compound C, confirming our conclusions. Overall, GLA can protect AML-12 cells from lipid metabolism disorder caused by PA via balancing autophagy and apoptosis mediated by the LKB1-AMPK-mTOR pathway. Consequently, GLA, as a dietary supplement, can help to prevent and treat NAFLD by regulating lipid metabolism and autophagy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.jafc.1c02596DOI Listing
July 2021

Efficient pullulan production by Aureobasidium pullulans using cost-effective substrates.

Int J Biol Macromol 2021 Jul 14;186:544-553. Epub 2021 Jul 14.

School of Biology and Basic Medical Sciences, Soochow University, Suzhou 215123, PR China. Electronic address:

In this study, cost-effective substrates such as cassava starch, corn steep liquor (CSL) and soybean meal hydrolysate (SMH) were used for pullulan production by Aureobasidium pullulans CCTCC M 2012259. The medium was optimized using response surface methodology (RSM) and artificial neural network (ANN) approaches, and analysis of variance indicated that the ANN model achieved higher prediction accuracy. The optimal medium predicted by ANN was used to produce high molecular weight pullulan in high yield. SMH substrates increased both biomass and pullulan titer, while CSL substrates maintained higher pullulan molecular weight. Results of kinetic parameters, key enzyme activities and intracellular uridine diphosphate glucose contents revealed the physiological mechanism of changes in pullulan titer and molecular weight using different substrates. Economic analysis of batch pullulan production using different substrates was performed, and the cost of nutrimental materials for CSL and SMH substrates was decreased by 46.1% and 49.9%, respectively, compared to the control using glucose and yeast extract as substrates, which could improve the competitiveness of pullulan against other polysaccharides in industrial applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijbiomac.2021.07.068DOI Listing
July 2021

Electrolyte Design for Lithium Metal Anode-Based Batteries Toward Extreme Temperature Application.

Adv Sci (Weinh) 2021 Jul 17:e2101051. Epub 2021 Jul 17.

Department of Chemical Engineering, Waterloo Institute of Nanotechnology, University of Waterloo, Waterloo, N2L 3G1, Canada.

Lithium anode-based batteries (LBs) are highly demanded in society owing to the high theoretical capacity and low reduction potential of metallic lithium. They are expected to see increasing deployment in performance critical areas including electric vehicles, grid storage, space, and sea vehicle operations. Unfortunately, competitive performance cannot be achieved when LBs operating under extreme temperature conditions where the lithium-ion chemistry fail to perform optimally. In this review, a brief overview of the challenges in developing LBs for low temperature (<0 °C) and high temperature (>60 °C) operation are provided followed by electrolyte design strategies involving Li salt modification, solvation structure optimization, additive introduction, and solid-state electrolyte utilization for LBs are introduced. Specifically, the prospects of using lithium metal batteries (LMBs), lithium sulfur (Li-S) batteries, and lithium oxygen (Li-O ) batteries for performance under low and high temperature applications are evaluated. These three chemistries are presented as prototypical examples of how the conventional low temperature charge transfer resistances and high temperature side reactions can be overcome. This review also points out the research direction of extreme temperature electrolyte design toward practical applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/advs.202101051DOI Listing
July 2021

A phosphatidic acid-binding lncRNA SNHG9 facilitates LATS1 liquid-liquid phase separation to promote oncogenic YAP signaling.

Cell Res 2021 Jul 15. Epub 2021 Jul 15.

MOE Laboratory of Biosystem Homeostasis and Protection, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China.

Long noncoding RNAs (lncRNAs) are emerging as a new class of important regulators of signal transduction in tissue homeostasis and cancer development. Liquid-liquid phase separation (LLPS) occurs in a wide range of biological processes, while its role in signal transduction remains largely undeciphered. In this study, we uncovered a lipid-associated lncRNA, small nucleolar RNA host gene 9 (SNHG9) as a tumor-promoting lncRNA driving liquid droplet formation of Large Tumor Suppressor Kinase 1 (LATS1) and inhibiting the Hippo pathway. Mechanistically, SNHG9 and its associated phosphatidic acids (PA) interact with the C-terminal domain of LATS1, promoting LATS1 phase separation and inhibiting LATS1-mediated YAP phosphorylation. Loss of SNHG9 suppresses xenograft breast tumor growth. Clinically, expression of SNHG9 positively correlates with YAP activity and breast cancer progression. Taken together, our results uncover a novel regulatory role of a tumor-promoting lncRNA (i.e., SNHG9) in signal transduction and cancer development by facilitating the LLPS of a signaling kinase (i.e., LATS1).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41422-021-00530-9DOI Listing
July 2021

Inactivation of the tumor suppressor p53 by long noncoding RNA RMRP.

Proc Natl Acad Sci U S A 2021 Jul;118(29)

Fudan University Shanghai Cancer Center, Fudan University, Shanghai 200032, China;

p53 inactivation is highly associated with tumorigenesis and drug resistance. Here, we identify a long noncoding RNA, the RNA component of mitochondrial RNA-processing endoribonuclease (RMRP), as an inhibitor of p53. RMRP is overexpressed and associated with an unfavorable prognosis in colorectal cancer. Ectopic RMRP suppresses p53 activity by promoting MDM2-induced p53 ubiquitination and degradation, while depletion of RMRP activates the p53 pathway. RMRP also promotes colorectal cancer growth and proliferation in a p53-dependent fashion in vitro and in vivo. This anti-p53 action of RMRP is executed through an identified partner protein, SNRPA1. RMRP can interact with SNRPA1 and sequester it in the nucleus, consequently blocking its lysosomal proteolysis via chaperone-mediated autophagy. The nuclear SNRPA1 then interacts with p53 and enhances MDM2-induced proteasomal degradation of p53. Remarkably, ablation of SNRPA1 completely abrogates RMRP regulation of p53 and tumor cell growth, indicating that SNRPA1 is indispensable for the anti-p53 function of RMRP. Interestingly and significantly, poly (ADP-ribose) polymerase (PARP) inhibitors induce RMRP expression through the transcription factor C/EBPβ, and RMRP confers tumor resistance to PARP inhibition by preventing p53 activation. Altogether, our study demonstrates that RMRP plays an oncogenic role by inactivating p53 via SNRPA1 in colorectal cancer.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1073/pnas.2026813118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8307289PMC
July 2021

Synthesis of Spirolactones via a BF·EtO-Promoted Cascade Annulation of α-Keto Acids and 1,3-Enynes.

Org Lett 2021 Jul 15. Epub 2021 Jul 15.

Department of Chemistry, Key Laboratory of Green and Precise Synthetic Chemistry, Ministry of Education, Huaibei Normal University, Huaibei, Anhui 235000, P. R. China.

A novel and effective method for the synthesis of spirolactones from readily available α-keto acids and 1,3-enynes is developed via a BF·EtO-promoted cascade annulation. This sequential process is conducted at room temperature, and it provides the functionalized spirolactones in good to excellent yield under metal-free conditions.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.orglett.1c01827DOI Listing
July 2021

Distinct roles of Fto and Mettl3 in controlling development of the cerebral cortex through transcriptional and translational regulations.

Cell Death Dis 2021 Jul 14;12(7):700. Epub 2021 Jul 14.

Center for Precision Medicine, School of Medicine and School of Biomedical Sciences, Huaqiao University, Xiamen, Fujian, China.

Proper development of the mammalian cerebral cortex relies on precise gene expression regulation, which is controlled by genetic, epigenetic, and epitranscriptomic factors. Here we generate RNA demethylase Fto and methyltransferase Mettl3 cortical-specific conditional knockout mice, and detect severe brain defects caused by Mettl3 deletion but not Fto knockout. Transcriptomic profiles using RNA sequencing indicate that knockout of Mettl3 causes a more dramatic alteration on gene transcription than that of Fto. Interestingly, we conduct ribosome profiling sequencing, and find that knockout of Mettl3 leads to a more severe disruption of translational regulation of mRNAs than deletion of Fto and results in altered translation of crucial genes in cortical radial glial cells and intermediate progenitors. Moreover, Mettl3 deletion causes elevated translation of a significant number of mRNAs, in particular major components in mA methylation. Our findings indicate distinct functions of Mettl3 and Fto in brain development, and uncover a profound role of Mettl3 in regulating translation of major mRNAs that control proper cortical development.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41419-021-03992-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8280107PMC
July 2021

Early Experience with the HeartMate Percutaneous Heart Pump from the SHIELD II Trial.

ASAIO J 2021 Jul 8. Epub 2021 Jul 8.

From the Tufts Medical Center, Cardiology Department, Boston, Massachusetts Montefiore Medical Center, Cardiology Department, Bronx, New York Piedmont Heart Institute, Cardiology Department, Atlanta, Georgia Mount Sinai Hospital, Cardiology Department, New York, New York Cardiology Department, University of Arizona, Tucson, Arizona Abbott Vascular, Santa Clara, California.

The HeartMate Percutaneous Heart Pump (PHP) is a novel circulatory support catheter delivering a self-expanding 24 French impeller across the aortic valve. The SHIELD II trial compares outcomes among heart failure patients undergoing high-risk percutaneous coronary intervention (HR-PCI) with the PHP versus Impella systems. The trial was halted in 2017 due to device malfunctions. We aimed to describe procedural, hemodynamic, and clinical outcomes among HR-PCI patients treated with PHP as part of the SHIELD II trial roll-in phase. Procedural, hemodynamic, and 90 day outcomes were assessed among patients undergoing HR-PCI with a left ventricular ejection fraction ≤35% and last patent coronary conduit, unprotected left main disease, or significant three vessel disease. The primary endpoint was the 90 day composite of cardiovascular death, myocardial infarction, stroke, repeat revascularization, major bleeding, new/worsening aortic regurgitation, and severe hypotension. Among 75 roll-in phase patients, PHP support duration was 101 ± 53 minutes with 2.5 ± 1.4 coronary lesions treated per patient. Compared with predevice values, the PHP system increased cardiac power and mean arterial pressure. Maximum recorded device flows were 0.4-6.2 L/minute with 26% (n = 19/73) and 9.6% (n = 7/73) of patients achieving peak flows above 3.5 or 5.0 L/minute, respectively. Five PHP device malfunction events (6.7%) were observed. At 90 days, the composite endpoint occurred in 24.3% (18/74) of patients. Early PHP experience demonstrated successful device performance in the majority of enrolled patients; however, unexpected malfunctions led to device revision. Completion of the SHIELD II trial will be required to confirm the safety and efficacy of this iteration of the PHP system in HR-PCI.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/MAT.0000000000001517DOI Listing
July 2021

Pre-invasion assessment of exotic bark beetle-vectored fungi to detect tree-killing pathogens.

Phytopathology 2021 Jul 14. Epub 2021 Jul 14.

University of Florida, 3463, School of Forest, Fisheries and Geomatics Sciences, Gainesville, Florida, United States;

Exotic diseases and pests of trees have caused continental-scale disturbances in forest ecosystems and industries, and their invasions are considered largely unpredictable. We tested the concept of pre-invasion assessment of not-yet invasive organisms, which enables empirical risk assessment of potential invasion and impact. Our example assesses fungi associated with Old World bark and ambrosia beetles and their potential to impact North American trees. We selected 55 Asian and European scolytine beetle species using host-use, economic, and regulatory criteria. We isolated 111 of their most consistent fungal associates and tested their effect on four important Southeastern American pine and oak species. Our test dataset found no highly virulent pathogens that should be classified as an imminent threat. Twenty-two fungal species were minor pathogens, which may require context-dependent response for their vectors at North American borders, while most of the tested fungi displayed no significant impact. Our results are significant in three ways: they ease the concerns over multiple overseas pests suspected of heightened potential risk; they provide basis for focus on the prevention of introduction and establishment of species that may be of consequence; and they demonstrate that pre-invasion assessment, if scaled up, can support practical risk assessment of exotic pathogens.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1094/PHYTO-01-21-0041-RDOI Listing
July 2021

Discordance in ER, PR, HER2, and Ki-67 Expression Between Primary and Recurrent/Metastatic Lesions in Patients with Primary Early Stage Breast Cancer and the Clinical Significance: Retrospective Analysis of 75 Cases.

Pathol Oncol Res 2021 9;27:599894. Epub 2021 Apr 9.

Department of Breast Surgery, Peking Union Medical College Hospital, Beijing, China.

The objective was to explore the discordance in the expression of the estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor 2 (HER2), and Ki-67 between primary and recurrent/metastatic lesions in patients with early stage breast cancer as well as the prognostic impact. Patients with early-stage primary breast cancer and confirmed recurrence/metastasis at Peking Union Medical College Hospital between January 2005 and August 2018 were screened. The details of discordance in each parameter between primary and recurrent/metastatic lesions and progression were recorded. Regression and survival analysis were applied to determine the association and clinical impact of the discordance. We evaluated 75 patients. The discordance rate of ER, PR, HER2, and Ki-67 expression was 9.3, 14.7, 14.7, and 21.5%, respectively. Additionally, 66.7, 11.8, 14.3, and 0% of patients with Luminal A, Luminal B, HER2, and triple-negative primary tumors presented with a different subtype for the recurrent/metastatic tumors, respectively. No statistical difference in progression-free survival was observed according to the subtype of the recurrent or metastatic breast cancer ( > 0.05). Among 69 patients for whom treatment was adjusted after recurrence or metastasis, 66 patients remained recurrence-free during the follow-up period. For patients with early-stage breast cancer, the ER, PR, HER2, and Ki-67 expression profile for recurrent/metastatic tumors does not always match that of the primary tumor. After adjusting treatment according to the receptor expression in recurrent/metastatic lesions, most patients remained progression-free during the follow-up period.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/pore.2021.599894DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8262146PMC
April 2021

Natural bioactive compounds from marine fungi (2017-2020).

J Asian Nat Prod Res 2021 Jul 12:1-28. Epub 2021 Jul 12.

School of Pharmacy, Jining Medical University, Rizhao 276800, China.

Secondary metabolites generated by marine fungi have relatively small molecular weights and excellent activities and have become an important source for developing drug lead compounds. The review summarizes the structures of novel small-molecule compounds derived from marine fungi in recent years; introduces representative monomers in antimicrobial, antitumor, anti-viral, and anti-neuritis aspects; and discusses their biological activities and molecular mechanisms. This review will act as a guide for further discovering marine-derived drugs with novel chemical structures and specific targeting mechanisms.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/10286020.2021.1947254DOI Listing
July 2021

Meta-Analysis of the Effect of Traditional Chinese Medicine Compounds Combined with Standard Western Medicine for the Treatment of Diabetes Mellitus Complicated by Coronary Heart Disease.

Evid Based Complement Alternat Med 2021 18;2021:5515142. Epub 2021 Jun 18.

Shaanxi University of Chinese Medicine, Xianyang 712046, China.

This study aimed to systematically evaluate the clinical efficacy and safety of traditional Chinese medicine (TCM) compounds combined with standard treatments for diabetes mellitus (DM) complicated by coronary heart disease (CHD). We performed a systematic and comprehensive search of the China Knowledge Network, WanFang, WeiPu, PubMed, and Web of Science, including Chinese and English articles, for randomized controlled trials (RCTs) assessing the use of Chinese herbal compounds for the treatment of DM complicated by CHD published before June 1, 2020. The literature was screened according to standard criteria. Risk assessment, based on the Jadad scale, was performed using the Review Manager5.3 software for meta-analysis. In total, 23 articles were selected, including 2405 cases. The meta-analysis showed that the combination of standard treatments with TCM compounds significantly improved the overall treatment efficacy for DM complicated by CHD (OR(odds ratio) = 4.39; 95% confidence interval (95% CI), 3.30-5.84; < 0.0001), fasting blood glucose level (mean difference (MD) = -1.04; 95% CI, -1.96 to -0.13; =0.03), total cholesterol level (MD = -1.16; 95% CI, -1.48 to -0.83; < 0.0001), triglyceride (MD = -0.46; 95% CI, -0.62 to -0.29; < 0.0001), low-density lipoprotein level (MD = -0.57; 95% CI, -0.87 to -0.27; =0.0002), high-density lipoprotein level (MD = 0.19; 95% CI, 0.12 to 0.26; =0.02), and electrocardiogram (OR = 4.20; 95% CI, 3.15 to 8.18; < 0.0001). In contrast, there was no improvement of 2-hour postprandial glucose level (MD = -1.03; 95% CI, -2.14 to 0.08; =0.07), or adverse reactions (OR = 0.53; 95% CI, 0.19 to 5.50; =0.21). We concluded that the combined therapy has some benefits in treating DM complicated by CHD. However, these results should be confirmed by further referenced evidence, high risk assessment, and lower publication bias.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2021/5515142DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8238557PMC
June 2021

Long Non-Coding RNA TP53TG1 Upregulates SHCBP1 to Promote Retinoblastoma Progression by Sponging miR-33b.

Cell Transplant 2021 Jan-Dec;30:9636897211025223

Department of Ophthalmology, 162798The First Affiliated Hospital of Xian Jiaotong University, Xi'an 710000, China.

Long non-coding RNA (lncRNA) TP53 target 1 (TP53TG1) is known to be strongly associated with tumor and cancer progression. However, its expression profile, unique role, and regulatory pathways in retinoblastoma (RB) are not known. Here, we revealed a large expression of TP53TG1 in RB tissues and cell lines. Conversely, we showed marked suppression of cell proliferation, migration, and invasion in TP53TG1 knocked down RB cells. Mechanistically, we established that TP53TG1 directly interacted with microRNA (miR)-33b in RB cells. Furthermore, TP53TG1 transcripts were found to be inversely correlated with miR-33b in RB tissues. We also showed that miR-33b suppression partly reversed the TP53TG1 knockdown mediated effects on tumor biology. Finally, TP53TG1 was shown to modulate the levels of SHC Binding and Spindle Associated 1 (SHCBP1), a direct target of miR-33b in RB cells. Based on the above data, we propose that TP53TG1 regulates RB progression via its modulation of the miR-33b/SHCBP1 pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/09636897211025223DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8278459PMC
July 2021

Design and operation of a transportable Rb atomic fountain clock.

Rev Sci Instrum 2021 May;92(5):054702

Key Laboratory of Quantum Optics, Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201811, China.

A transportable fountain clock with high reliability is important for high-precision time-frequency measurements. Because of its relatively small cold atoms' collision frequency shift and ease of attaining high quantum state preparation efficiency, the rubidium atomic fountain clock has an indicated higher stability and reliability. This paper reports the design and operation of a transportable rubidium atomic fountain clock developed by the Shanghai Institute of Optical and Fine Mechanics, Chinese Academy of Science. After being transported more than 1000 km from Shanghai to the Changping Campus of the National Institute of Metrology, China, the optical platform and other hardware of the fountain clock did not need to be adjusted. The rubidium fountain clock maintained a stability of 4.0 × 10τ, reaching 5.0 × 10 at 300 000 s. After transportation, the rubidium fountain clock and a cesium fountain clock (NIM5) were operated together against the reference frequency of a hydrogen maser. In three separate operating periods, over a total of nearly three months, the average frequency repeatability of the rubidium fountain was less than 3.8 × 10.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1063/5.0047715DOI Listing
May 2021

Increased miR-6875-5p inhibits plasmacytoid dendritic cell differentiation via the STAT3/E2-2 pathway in recurrent spontaneous abortion.

Mol Hum Reprod 2021 Jul 7. Epub 2021 Jul 7.

Department of Obstetrics and Gynecology, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Jinan, Shandong, China.

Recurrent spontaneous abortion (RSA) is a common complication of early pregnancy. Dendritic cells (DCs) are thought to confer fetal-maternal immunotolerance and play a crucial role in ensuring a successful pregnancy. A decrease of plasmacytoid dendritic cells (pDCs) was found to be involved in RSA, but the underlying mechanisms of decreased pDC in RSA remain unclear. MicroRNAs (miRNAs) play critical roles in RSA as well as the development, differentiation and functional regulation of pDCs; however, the regulatory effect of miRNAs on pDC in RSA has not been fully investigated. Here we demonstrated that both the proportion of pDC and signal transducer and activator of transcription (STAT3)/transcription factor 4 (Tcf4/E2-2) expression decreased in the peripheral blood mononuclear cells and decidua of patients with RSA compared to those with normal pregnancy (NP), and there was a significantly positive correlation between pDC and STAT3 mRNA. MiRNA microarray assay and quantitative reverse transcription PCR results showed that miR-6875-5p expression was markedly increased in women with RSA and negatively correlated with mRNA expression level of STAT3. Up-regulated miR-6875-5p could sensitively discriminate patients with RSA from NP subjects. Overexpression of miR-6875-5p significantly down-regulated the mRNA expression of STAT3 and E2-2 as well as the protein and phosphorylation level of STAT3, while miR-6875-5p knockdown showed opposite results. Dual luciferase reporter verified that miR-6875-5p regulated STAT3 expression by directly binding to its 3'untranslated region. Overall, our results suggested that increased miR-6875-5p is involved in RSA by decreasing the differentiation of pDCs via inhibition of the STAT3/E2-2 signaling pathway. miR-6875-5p may be explored as a promising diagnostic marker and therapeutic target for RSA.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/molehr/gaab044DOI Listing
July 2021

Phylogeny and Comparative Analysis for the Plastid Genomes of Five (Liliaceae).

Biomed Res Int 2021 18;2021:6648429. Epub 2021 Jun 18.

CAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization & Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041 Sichuan, China.

Species of (Liliaceae) are of great horticultural importance and are distributed across Europe, North Africa, and Asia. The Tien Shan Mountain is one of the primary diversity centres of , but the molecular studies of species from this location are lacking. In our study, we assembled four plastid genomes from the Tien Shan Mountains, , , , and , combined with the plastid genome of to compare against other Liliaceae plastid genomes. We focussed on the species diversity and evolution of their plastid genomes. The five plastid genomes proved highly similar in overall size (151,691-152,088 bp), structure, gene order, and content. With comparative analysis, we chose 7 mononucleotide SSRs from the species that could be used in further population studies. Phylogenetic analyses based on 24 plastid genomes robustly supported the monophyly of and the sister relationship between and , . , , and were clustered together, and was clustered with , with our results clearly demonstrating the relationships between these five species. Our results provide a more comprehensive understanding of the phylogenomics and comparative genomics of .
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2021/6648429DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8235973PMC
June 2021

An atlas-guided automatic planning approach for rectal cancer intensity-modulated radiotherapy.

Phys Med Biol 2021 Jul 23;66(15). Epub 2021 Jul 23.

Department of Radiation Oncology, Fudan University Shanghai Cancer Center, Shanghai, People's Republic of China.

We try to develop an atlas-guided automatic planning (AGAP) approach and evaluate its feasibility and performance in rectal cancer intensity-modulated radiotherapy. The developed AGAP approach consisted of four independent modules: patient atlas, similar patient retrieval, beam morphing (BM), and plan fine-tuning (PFT) modules. The atlas was setup using anatomy and plan data from Pinnacle auto-planning (P-auto) plans. Given a new patient, the retrieval function searched the top similar patient by a generic Fourier descriptor algorithm and retrieved its plan information. The BM function generated an initial plan for the new patient by morphing the beam aperture from the top similar patient plan. The beam aperture and calculated dose of the initial plan were used to guide the new plan optimization in the PFT function. The AGAP approach was tested on 96 patients by the leave-one-out validation and plan quality was compared with the P-auto plans. The AGAP and P-auto plans had no statistical difference for target coverage and dose homogeneity in terms of( = 0.76) and homogeneity index ( = 0.073), respectively. The CI index showed they had a statistically significant difference. But the ΔCI was both 0.02 compared to the perfect CI index of 1. The AGAP approach reduced the bladder mean dose by 152.1 cGy ( < 0.05) andby 0.9% ( < 0.05), and slightly increased the left and right femoral head mean dose by 70.1 cGy ( < 0.05) and 69.7 cGy ( < 0.05), respectively. This work developed an efficient and automatic approach that could fully automate the IMRT planning process in rectal cancer radiotherapy. It reduced the plan quality dependence on the planner experience and maintained the comparable plan quality with P-auto plans.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1088/1361-6560/ac127dDOI Listing
July 2021

A Computational Metabolic Model for Engineered Production of Resveratrol in .

ACS Synth Biol 2021 Jul 8. Epub 2021 Jul 8.

Department of Electrical and Computer Engineering, Utah State University, Logan, Utah 84322-4120, United States.

Although engineered microbial production of natural compounds provides a promising alternative method to plant production and extraction, the process can be inefficient and ineffective in terms of time and cost. To render microbial systems profitable and viable, the process must be optimized to produce as much product as possible. To this end, this work illustrates the construction of a new probabilistic computational model to simulate the microbial production of a well-known cardioprotective molecule, resveratrol, and the implementation of the model to enhance the yield of the product in . This model identified stilbene synthase as the limiting enzyme and informed the effects on changes in concentration and source of this enzyme. These parameters, when employed in a laboratory system, were able to improve the titer from 62.472 mg/L to 172.799 mg/L, demonstrating the model's ability to produce a useful simulation of a dynamic microbial resveratrol production system.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acssynbio.1c00163DOI Listing
July 2021
-->