Publications by authors named "Zhen Zhang"

3,226 Publications

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Life cycle assessment of bioelectrochemical and integrated microbial fuel cell systems for sustainable wastewater treatment and resource recovery.

J Environ Manage 2022 Aug 8;320:115778. Epub 2022 Aug 8.

School of Materials and Environmental Engineering, Shenzhen Polytechnic, Guangdong, 518055, China.

Bioelectrochemical system (BES) is an emerging technology that can treat wastewater via microbial activity while producing energy simultaneously. The system can couple with conventional systems to improve system performance. This study aims to compare the environmental performance of BES and the integrated microbial fuel cell (MFC) systems via a life cycle assessment methodology and identify the major environmental hotspots of the system. Fifteen treatment options are assessed with the ReCiPe 2016 characterization method using SimaPro 9.2 software. The results show double chamber air-cathode microbial electrolysis cell (MEC1) and membrane distillation integrated MFC (MD + MFC) treatment options present as the most environmental favourable among the BES and integrated MFC systems, respectively, due to the offset of the environmental loads from the avoided impacts contributed by their value-added by-product, which is hydrogen fuel for MEC1 and tap water for MD + MFC. Electricity consumption dominates the environmental loads of all the BES options for up to 90% of the global warming impact category. The environmental benefits from the electricity generation of BES are minor (i.e., MFC: 0.01-2% while microbial desalination cell: 0.01-7% of the total environmental impact in a system) to offset the environmental loads incurred by the system. Platinum-based cathode incurs 2.5-24 times higher environmental burdens than non-platinum configurations in MFC under the human carcinogenic toxicity impact category. In line with Sustainable Development Goals 6 and 13, this study provides scientific references to wastewater treatment stakeholders in selecting suitable BES and integrated MFC systems to improve water sanitation and address climate change simultaneously.
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http://dx.doi.org/10.1016/j.jenvman.2022.115778DOI Listing
August 2022

Dual-Scale Integration Design of Sn-ZnO Catalyst Towards Efficient and Stable CO Electroreduction.

Adv Mater 2022 Aug 10:e2204637. Epub 2022 Aug 10.

Department of Chemical Engineering, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada.

Electrochemical CO reduction to CO is a potential sustainable strategy for alleviating CO emissions and producing valuable fuels. In the quest to resolve its current problems of low energy efficiency and insufficient durability, we propose a dual-scale design strategy by implanting non-noble active Sn-ZnO heterointerface inside the nanopores of high-surface-area carbon nanospheres ([email protected]). The metal d-bandwidth tuning of Sn and ZnO alters the extent of substrate-molecule orbital mixing, facilitating the break of the *COOH intermediate and the yield of CO. Furthermore, the confinement effect of tailored nanopores results in beneficial pH distribution in the local environment around the Sn-ZnO nanoparticles and protects them against leaching and aggregating. Through integrating electronic and nanopore scale control, [email protected] achieves a quite low potential of -0.53 V versus reversible hydrogen electrode with 91% Faradaic efficiency for CO and ultralong stability of 240 hours. This work provides proof of concept for the multiscale design of electrocatalysts. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/adma.202204637DOI Listing
August 2022

Clinical Results of Utilizing the Satellite Rod Technique in Treating Ankylosing Spondylitis Kyphosis.

Orthop Surg 2022 Aug 10. Epub 2022 Aug 10.

Department of Orthopaedics, Chinese People's Liberation Army General Hospital (301 Hospital), Beijing, China.

Objective: According to the literature, there are no clinical reports documenting the use of the satellite rod technique in the treatment of ankylosing spondylitis kyphosis. The purpose of this retrospective study was to compare the clinical outcome of patients with ankylosing spondylitis kyphosis who adopted satellite rods versus those who did not.

Methods: Patients with ankylosing spondylitis kyphosis who underwent one or two-level pedicle subtraction osteotomy (PSO) were reviewed, and total of 119 patients (112 males and seven females, average age 39.89 ± 6.61 years) were eligible and included in this present study. Anterior-posterior and lateral full-length spine X-ray films were performed preoperatively and at the two-year follow-up visit. Global kyphosis (GK), lumbar lordosis (LL), thoracolumbar kyphosis (TLK), thoracic kyphosis (TK), and osteotomy angle (OA) were measured. The complications of every group of patients were collected. Pre- and postoperative health-related quality of life instruments, including the Bath Ankylosing Spondylitis Functional Index (Basfi) and Scoliosis Research Society outcomes instrument-22 (SRS-22), were recorded. The patients were divided into three groups based on features of their osteotomy including PSO levels and whether the satellite rod technique was applied. Patients who underwent one-level PSO without the satellite rod technique were categorized in the one-level group. Patients who underwent one-level PSO with the satellite rod technique were classified in the satellite rod group. Patients who underwent two-level PSO without the satellite rod technique were included in the two-level group. The paired sample t test was used to compare pre- and postoperative parameters. One-way ANOVA was performed for multiple group comparisons.

Results: The average follow-up time is 29.31 ± 3.66 months. The patients' GK were significantly improved from 46.84 ± 20.37 degree to 3.31 ± 15.09 degree. OS achieved through each osteotomy segment of one-level group (39.78 ± 12.29 degree) and satellite rods group (42.23 ± 9.82 degree), was larger than that of two-level group (34.73 ± 7.54 and 28.85 ± 7.26 degree). There was no significant difference between the one-level group and the satellite rod group in achieving the OS. Thirteen patients experienced different complications (10.92%). Three patients experienced rod fracture in the one-level group. There was no rod fracture or screw failure in the satellite rod group or the two-level group.

Conclusion: The satellite rod technique is also recommended for patients who undergo PSO osteotomy to correct ankylosing spondylitis kyphosis deformities.
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http://dx.doi.org/10.1111/os.13427DOI Listing
August 2022

Radical annulation of a designed diene system: access to nitro-benzo[]azepines.

Chem Commun (Camb) 2022 Aug 9. Epub 2022 Aug 9.

College of Chemistry and Chemical Engineering, Yantai University, Yantai, 264005, Shandong, P. R. China.

Herein, we describe a novel ON˙-triggered ordered addition 7- cyclization reaction with excellent chemo- and regioselectivity. With such a strategy, structurally diverse nitro-benzo[]azepines were prepared with 28 examples. Large-scale operation and handy -Ts and -Cbz deprotection reveal the promising utility of this methodology. Mechanistic studies suggest that the reaction proceeds through a radical pathway.
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http://dx.doi.org/10.1039/d2cc02688kDOI Listing
August 2022

DNA compaction and dynamic observation in a nanopore gated sub-attoliter silicon nanocavity.

Nanoscale 2022 Aug 9. Epub 2022 Aug 9.

Division of Solid-State Electronics, Department of Electrical Engineering, Uppsala University, SE-751 03 Uppsala, Sweden.

Confinement of biopolymers inside volumes with micro- or nanoscale lateral dimensions is ubiquitous in nature. Investigating the behavior of biopolymers in a confined environment is essential to improve our basic understanding in life sciences. In this work, we present a nanopore gated sub-attoliter silicon nanocavity device, which allows DNA compaction similar to that in virus capsids. Single DNA molecules can be electrically driven into the nanocavity, and then get compacted inside the nanocavity under certain conditions. The dynamic fluctuations of the compacted DNA can be monitored ionic current measurements. The mechanism for the DNA compaction is elucidated by varying the DNA length or concentration, voltage polarity, nanocavity dimensions and ionic strength. Furthermore, Brownian dynamics simulations reveal the dynamic fluctuations of the compacted DNA, which are reflected in the measured ionic current. Our nanocavity device is anticipated to provide a controlled environment in extremely small volumes for investigating the physics of confined biopolymers.
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http://dx.doi.org/10.1039/d2nr02260eDOI Listing
August 2022

An All-Inside Repair Technique for Unstable Type-IV Lateral Meniscal Posterior Root Tear.

Arthrosc Tech 2022 Jul 21;11(7):e1317-e1320. Epub 2022 Jun 21.

Sports Medicine Department, Beijing Key Laboratory of Sports Injuries, Peking University Third Hospital, Beijing, China.

Lateral meniscal posterior root tears are defined as tears of meniscus within 9 mm from the bony root attachment. Unrepaired root tears significantly compromise the biomechanical functions of the meniscus, resulting in early and accelerated osteoarthritis. Several authors have described various techniques to reattach the posterior root of the lateral meniscus, and decent short-term results have been observed. Although most previous techniques are capable of repairing radial root tears, difficulties are encountered when repairing oblique type IV tears. In this technique note, the author describes an efficient side-to-side repair technique using the FAST-FIX system, which is practical in repairing the type IV tears of the lateral meniscus. This technique includes certain sequence of the stitches and skills of controlling the root remnant, which makes it easy to penetrate the meniscus and to achieve anatomic repair.
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http://dx.doi.org/10.1016/j.eats.2022.03.016DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9353532PMC
July 2022

Adjustment of the GRACE score by the triglyceride glucose index improves the prediction of clinical outcomes in patients with acute coronary syndrome undergoing percutaneous coronary intervention.

Cardiovasc Diabetol 2022 Aug 5;21(1):145. Epub 2022 Aug 5.

Department of Cardiology, The Third People's Hospital of Chengdu, Affiliated Hospital of Southwest Jiaotong University, Chengdu, 610014, Sichuan, China.

Background: The Global Registry of Acute Coronary Events (GRACE) score derived from clinical parameters at the time of hospital discharge is a powerful predictor of long-term mortality and reinfarction after acute coronary syndrome (ACS). The triglyceride glucose (TyG) index, which is a simple and reliable surrogate marker of insulin resistance, has been demonstrated to be an independent predictor of long-term adverse major adverse cardiac events, irrespective of diabetes mellitus. We investigate whether the addition of the TyG index improves the predictive ability of the GRACE score after percutaneous coronary intervention (PCI) in ACS patients regardless of diabetes mellitus.

Method: A retrospective cohort of 986 ACS patients undergoing PCI was enrolled in the present analyses. The GRACE score for discharge to 6 months and the TyG index were calculated. The primary endpoint was the composite of MACEs, including all-cause death and nonfatal myocardial infarction. Patients were stratified according to the primary endpoint and the tertiles of the TyG index. Cumulative curves were calculated using the Kaplan-Meier method. Multivariate Cox regression was adopted to identify predictors of MACEs. The predictive value of the GRACE score alone and combined with the TyG index or fasting blood glucose (FBG) was estimated by the area under the receiver‑operating characteristic curve, likelihood ratio test, Akaike's information criteria, continuous net reclassification improvement (NRI), and integrated discrimination improvement (IDI). Internal validation was assessed using the means of bootstrap method with 1000 bootstrapped samples.

Results: During a median follow-up of 30.72 months ((interquartile range, 26.13 to 35.07 months), 90 patients developed MACEs, more frequently in the patients with a higher TyG index. Multivariate Cox hazards regression analysis found that the TyG index, but not FBG was an independent predictor of MACEs (hazard ratio 1.6542; 95% CI 1.1555-2.3681; P = 0.006) in all types of ACS regardless of diabetes mellitus when included in the same model as GRACE score. Furthermore, Kaplan-Meier analysis revealed that the incidence of the primary endpoint rose with increasing TyG index tertiles (log-rank, P < 0.01). Adjustment the GRACE score by the TyG index improved the predictive ability for MACEs (increase in C-statistic value from 0.735 to 0.744; NRI, 0.282, 95% CI 0.028-0.426, P = 0.02; IDI, 0.019, 95% CI 0.004-0.046, P = 0.01). Likelihood ratio test showed that the TyG index significantly improved the prognostic ability of the GRACE score (χ = 12.37, 1 df; P < 0.001). The results remained consistent when the models were confirmed by internal bootstrap validation method.

Conclusion: The TyG index, but not FBG is an independent predictor of long-term MACEs after PCI in all types of ACS patients regardless of diabetes mellitus after adjusting for the GRACE score, and improves the ability of the GRACE score to stratify risk and predict prognosis of ACS patients undergoing PCI.
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http://dx.doi.org/10.1186/s12933-022-01582-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9356419PMC
August 2022

Prevalence and antimicrobial-resistant characterization of isolated from ready-to-eat rice products in Eastern China.

Front Microbiol 2022 15;13:964823. Epub 2022 Jul 15.

Hainan Institute, Zhejiang University, Hangzhou, China.

is a major food-borne bacterial pathogen in the world, which can cause diarrhea and emetic syndrome. This study aimed to reveal the quantitative prevalence of in ready-to-eat (RTE) rice products in Eastern China and to gain essential information on the characteristics of isolates. A total of 91 out of the 1071 samples were positive for . The contamination level of in 0.5 % of RTE rice product samples outnumbered 10 CFU/g. The number of attained 10-10 CFU/g in one sample. The distribution patterns of virulence genes in isolates were identified. 84.6% of the isolates had at least one enterotoxin or emetic toxin gene. The predominant pattern was XXV. 9.9% of isolates belonged to it and possessed one enterotoxin gene . The occurrence rate of and was 36.3% and 47.3%, respectively. Antimicrobial susceptibility tests revealed a high resistance rate toward penicillin, and 23.1% of the isolates were multi-drug resistant. isolates were genotyped by using ERIC-PCR. 89 genotypes were determined. The Hunter Gaston Discriminatory Index (HGDI) attained 0.9995. Relationships analysis revealed that Group A isolates tended to carry , , , , and show resistance to penicillin/trimethoprim/sulfamethoxazole. This study was useful for updating the knowledge of the contamination status of in RTE rice products in China.
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http://dx.doi.org/10.3389/fmicb.2022.964823DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9344136PMC
July 2022

Circulating osteocalcin is associated with time in range and other metrics assessed by continuous glucose monitoring in type 2 diabetes.

Diabetol Metab Syndr 2022 Aug 4;14(1):109. Epub 2022 Aug 4.

Department of Endocrinology, Jinling Hospital, Southern Medical University, Nanjing, China.

Background: Osteocalcin, a protein secreted mainly by mature osteoblasts, has been shown to be involved in glucose metabolism through various pathways. However, few studies has explored the association between osteocalcin and Time in range (TIR). Continuous glucose monitoring (CGM) -derived metrics, such as TIR and other indexes have been gradually and widely used in clinical practice to assess glucose fluctuations. The main purpose of this study was to investigate the correlation between osteocalcin and indexes from CGM in patients with type 2 diabetes mellitus (T2DM).

Method: The total number of 376 patients with T2D were enrolled, all of them performed three consecutive days of monitoring. They were divided into four groups on account of the quartile of osteocalcin. Time in range, Time below range (TBR), Time above range(TAR) and measures of glycemic variability (GV) were assessed for analysing. After a 100 g standard steamed bread meal, blood glucose (Glu0h Glu0.5 h, Glu1h, Glu2h, GLu3h), C-peptide (Cp0h, Cp0.5 h, Cp1h, Cp2h, Cp3h), serum insulin (INS0h, INS0.5 h, INS1h, INS2h, INS3h) concentrations at different time points were obtained. HOMA-IS, HOMA-βwas calculated to evaluate insulin sensitivity and insulin secreting of the participants.

Results: Patients with higher osteocalcin level had higher TIR (P < 0.05). Spearman correlation analysis showed that osteocalcin was positively correlated with TBR (although the P value for TBR was greater than 0.05) (r = 0.227, P < 0.001 r = 0.068, P = 0.189) and negatively correlated with TAR (- 0.229, P < 0.001). Similarly, there was a negative correlation between osteocalcin and glycemic variability (GV) indicators, including SD, MBG, MODD, ADDR, and MAGE (P value of MAGE > 0.05). Multiple stepwise regression showed that osteocalcin was an independent contributor to TIR, TAR and HOMA-IS.

Conclusion: Circulating osteocalcin is positively correlated with TIR and negatively correlated with MODD, ADDR, and MAGE. Osteocalcin may have a beneficial impact on glucose homeostasis in T2DM patients.
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http://dx.doi.org/10.1186/s13098-022-00863-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9351112PMC
August 2022

Spray drift evaluation with point clouds data of 3D LiDAR as a potential alternative to the sampling method.

Front Plant Sci 2022 18;13:939733. Epub 2022 Jul 18.

Centre for Pesticide Application and Safety, The University of Queensland, Brisbane, QLD, Australia.

Spray drift is an inescapable consequence of agricultural plant protection operation, which has always been one of the major concerns in the spray application industry. Spray drift evaluation is essential to provide a basis for the rational selection of spray technique and working surroundings. Nowadays, conventional sampling methods with passive collectors used in drift evaluation are complex, time-consuming, and labor-intensive. The aim of this paper is to present a method to evaluate spray drift based on 3D LiDAR sensor and to test the feasibility of alternatives to passive collectors. Firstly, a drift measurement algorithm was established based on point clouds data of 3D LiDAR. Wind tunnel tests included three types of agricultural nozzles, three pressure settings, and five wind speed settings were conducted. LiDAR sensor and passive collectors (polyethylene lines) were placed downwind from the nozzle to measure drift droplets in a vertical plane. Drift deposition volume on each line and the number of LiDAR droplet points in the corresponding height of the collecting line were calculated, and the influencing factors of this new method were analyzed. The results show that 3D LiDAR measurements provide a rich spatial information, such as the height and width of the drift droplet distribution, etc. High coefficients of determination ( > 0.75) were observed for drift points measured by 3D LiDAR compared to the deposition volume captured by passive collectors, and the anti-drift IDK12002 nozzle at 0.2 MPa spray pressure has the largest value, which is 0.9583. Drift assessment with 3D LiDAR is sensitive to droplet density or drift mass in space and nozzle initial droplet spectrum; in general, larger droplet density or drift mass and smaller droplet size are not conducive to LiDAR detection, while the appropriate threshold range still needs further study. This study demonstrates that 3D LiDAR has the potential to be used as an alternative tool for rapid assessment of spray drift.
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http://dx.doi.org/10.3389/fpls.2022.939733DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9340218PMC
July 2022

Reconfigurable hyperbolic polaritonics with correlated oxide metasurfaces.

Nat Commun 2022 Aug 3;13(1):4511. Epub 2022 Aug 3.

Department of Physics and Astronomy, University of Georgia, Athens, GA, 30602, USA.

Polaritons enable subwavelength confinement and highly anisotropic flows of light over a wide spectral range, holding the promise for applications in modern nanophotonic and optoelectronic devices. However, to fully realize their practical application potential, facile methods enabling nanoscale active control of polaritons are needed. Here, we introduce a hybrid polaritonic-oxide heterostructure platform consisting of van der Waals crystals, such as hexagonal boron nitride (hBN) or alpha-phase molybdenum trioxide (α-MoO), transferred on nanoscale oxygen vacancy patterns on the surface of prototypical correlated perovskite oxide, samarium nickel oxide, SmNiO (SNO). Using a combination of scanning probe microscopy and infrared nanoimaging techniques, we demonstrate nanoscale reconfigurability of complex hyperbolic phonon polaritons patterned at the nanoscale with high resolution. Hydrogenation and temperature modulation allow spatially localized conductivity modulation of SNO nanoscale patterns, enabling robust real-time modulation and nanoscale reconfiguration of hyperbolic polaritons. Our work paves the way towards nanoscale programmable metasurface engineering for reconfigurable nanophotonic applications.
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http://dx.doi.org/10.1038/s41467-022-32287-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9349304PMC
August 2022

Efficacy of ultrasound-guided quadratus lumborum block for postoperative analgesia after hip arthroplasty: a meta-analysis of randomized controlled trials.

J Arthroplasty 2022 Jul 31. Epub 2022 Jul 31.

Department of Orthopedics, The First Affiliated Hospital of Dalian Medical University, Dalian, 116011, China. Electronic address:

Background: Ultrasound-guided quadratus lumborum (QL) block as a novel regional anesthetic technique was proposed in 2007 that can be applied in patients following hip arthroplasty. This study aimed to evaluate the efficacy of the QL block for pain control in patients undergoing hip arthroplasty.

Methods: We performed a comprehensive search of PubMed, Web of Science, Scopus, Cochrane Library, Embase databases, Google Scholar, and CNKI for randomized controlled trials (RCTs) up to December 2021. According to the inclusion and exclusion criteria established in advance, "QL block" and "hip arthroplasty" related MesH terms and free-text words were used.

Results: Our meta-analysis included 11 RCTs involving a total of 830 patients between 2018 and 2021. The results indicated that compared to the non-QL block group, VAS score at mobilization in the OL block group demonstrated statistical and clinical significance at all time points (12, 24, and 48 hours), but VAS score at rest failed to reach the MCID (minimal clinically important difference). Meanwhile, opioid consumption in the OL block group only demonstrated statistical and clinical significance at 48 hours postoperatively, but did not reach the MCID at 12 or 24 hours postoperatively. The QL block increased satisfaction scores. There was a statistically significant reduction in the incidence of PONV (postoperative nausea and vomiting), but no difference in the incidence of pruritus and urinary retention.

Conclusions: The QL block significantly reduced postoperative VAS score at mobilization, and opioid consumption at 48 hours in patients after hip arthroplasty compared to no block, which reached the MCID. The QL block also decreased PONV and increased satisfaction scores. Although these are promising results, the clinical relevance of the efficacy of the QL block remains to be further understood as larger studies are needed.
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http://dx.doi.org/10.1016/j.arth.2022.07.013DOI Listing
July 2022

Islet-1 Mesenchymal Stem Cells-Derived Exosome-Incorporated Angiogenin-1 Hydrogel for Enhanced Acute Myocardial Infarction Therapy.

ACS Appl Mater Interfaces 2022 Aug 3. Epub 2022 Aug 3.

Guangdong Provincial Key Laboratory of Shock and Microcirculation, Dongguan Hospital of Southern Medical University, Southern Medical University, Guangzhou, Guangdong 510080, China.

Although stem cell-derived exosomes have been recognized as new candidates for cell-free treatment in myocardial infarction (MI), the challenge to improve the exosome retention in ischemic tissue remains. Our previous research indicated that islet-1(ISL1) overexpression enhances the paracrine function of mesenchymal stem cells (MSCs) and promotes angiogenesis in a model of MI. In this study, genetically engineered ISL1-MSC-derived exosomes (ISL1-MSCs-Exo) were collected, and the contents were analyzed by exosomal RNA sequencing. Next, we investigated if ISL1-MSCs-Exo could exert therapeutic effects and their incorporation into a new angiogenin-1 hydrogel (Ang-1 gel) could boost the retention of exosomes and further enhance their protective effects. Our results demonstrated that ISL1-MSCs-Exo could play a therapeutic role in vitro and in vivo, which might be due to changed exosomal contents. Ang-1 gel increased the retention and enhanced the anti-apoptosis, proliferation, and angiogenic capacity of ISL1-MSCs-Exo in endothelial cells. Echocardiography revealed that Ang-1 gel significantly augment the therapeutic effects of ISL1-MSCs-Exo for MI. The main mechanism might result from increased retention of ISL1-MSCs-Exo, herein enhanced pro-angiogenetic effects in an ischemic heart. Taken together, our findings indicated that ISL1-MSCs-Exo had endothelium-protective and pro-angiogenic abilities alone and Ang-1 gel could notably retain ISL1-MSCs-Exo at ischemic sites, which improved the survival and angiogenesis of endothelial cells and accelerated the recovery of MI. These results not only shed light on the therapeutic mechanism of ISL1-MSCs-Exo incorporated with Ang-1 gel but also offer a promising therapeutic option for ischemic disease.
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http://dx.doi.org/10.1021/acsami.2c04686DOI Listing
August 2022

Context-guided entropy minimization for semi-supervised domain adaptation.

Neural Netw 2022 Jul 23;154:270-282. Epub 2022 Jul 23.

University of California, Santa Barbara, USA. Electronic address:

Semi-Supervised Domain Adaptation has been widely studied with various approaches to address domain shift with labeled source-domain data combined with scarcely labeled target-domain data. Model adaptation is becoming promising with a paradigm of source pre-training and target fine-tuning, which eliminates the simultaneous availability of data from both domains and makes for data privacy. Among the model adaptation methods, Entropy Minimization (EM) is popularly incorporated to encourage a low-density separation on target samples. However, EM tends to brutally force models to make over-confident predictions, which could make the models collapse with deteriorated performance. In this paper, we first study the over-confidence of EM with a quantitative analysis, which shows the importance of capturing the dependency among labels. To address this issue, we propose to guide EM via longitudinal self-distillation. Specifically, we produce a dynamic "teacher" label distribution during training by constructing a graph on target data and perform pseudo-label propagation to encourage the "teacher" distribution to capture context category dependency based on a global data structure. Then EM is guided longitudinally by distilling the learned label distribution to combat the brute-force over-confidence. Extensive experiments demonstrate the effectiveness of our methods.
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http://dx.doi.org/10.1016/j.neunet.2022.07.011DOI Listing
July 2022

Gonadotropin-releasing hormone agonists for ovarian protection during breast cancer chemotherapy: a systematic review and meta-analysis.

Menopause 2022 Aug 2. Epub 2022 Aug 2.

From the Department of Breast Surgery, Luoyang Central Hospital Affiliated to Zhengzhou University, Luoyang, China.

Importance: The increasing trend of delaying childbirth means that more women are being diagnosed with breast cancer before having given birth to their desired number of children. Although chemotherapy can significantly improve the prognosis of this population, it also causes ovarian damage, including premature ovarian insufficiency and infertility. Gonadotropin-releasing hormone agonists (GnRHa) have shown promising fertility protective activity in premenopausal women, but their clinical usage remains controversial.

Objective: Here, we conducted a meta-analysis to assess the efficacy of GnRHa when administered concurrently with chemotherapy that included cyclophosphamide in the prevention of chemotherapy-induced ovarian damage in premenopausal women.

Evidence Review: An extensive literature search was performed using the PubMed, Embase, and Cochrane databases. Pooled odds ratios (ORs) with 95% confidence intervals (CIs) were determined.

Findings: Eleven randomized controlled trials with a total of 1,219 participants were included in the analyses. A significantly higher number of women treated with GnRHa experienced the resumption of ovarian function after chemotherapy than those who did not receive this treatment (OR, 3.04; 95% CI, 1.87-4.94; P < 0.001). Regarding spontaneous pregnancy, a statistically significant difference was observed only in hormone receptor-negative participants (OR, 2.06; 95% CI, 1.03-4.11; P = 0.04).

Conclusions And Relevance: When treating premenopausal women with breast cancer, the administration of GnRHa concurrently with chemotherapy appeared to improve the resumption rate of ovarian function; however, the spontaneous pregnancy rate only improved in hormone receptor-negative patients. Thus, the use of GnRHa during chemotherapy may represent a feasible strategy for preserving ovarian function in women with breast cancer.
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http://dx.doi.org/10.1097/GME.0000000000002019DOI Listing
August 2022

Effect of Tranexamic Acid on Blood Management during a High Tibial Osteotomy: A Systematic Review and Meta-analysis.

Orthop Surg 2022 Aug 1. Epub 2022 Aug 1.

West China Hospital, Sichuan University, Chengdu, China.

This study aimed to evaluate the efficiency and safety of tranexamic acid for blood management during high tibial osteotomy (HTO). A systematic search was conducted in Medline, Embase, and the Cochrane library database. Six studies and 208 patients were included in this meta-analysis using Review Manager V.5.3 and Stata 15.1 software. For primary outcomes, tranexamic acid lowered the total blood loss (WMD = -219.47, 95% CI [-355.61, -83.33], P = 0.002). For secondary outcomes, a significant reduction was found for decreased hemoglobin (POD1: WMD = -9.86, 95% CI [-13.45, -6.28], P < 0.05; POD2: WMD = -8.41, 95% CI [-11.50, -5.32], P < 0.05; POD5: WMD = -11.48, 95% CI [-14.56, -8.39], P < 0.05) and drainage (total: WMD = -105.93, 95% CI [-187.08, -24.78], P < 0.05; POD1: WMD = -122.195, 95% CI [-168.902, -75.488], P < 0.05). The sex difference (male/female ratio) was determined (total blood loss: P = 0.025; total drainage amount: p = 0.018) using meta-regression analysis. Females benefited more from tranexamic acid in terms of total blood loss (M/F > 40%: WMD = -53.11, 95% CI [-100.16, -6.05], P = 0.03; 40% ≥ M/F ≥ 20%: WMD = -362.20, 95% CI [-423.96, -300.45], P < 0.05; M/F < 20%: WMD = -263.00, 95% CI [-277.17, -248.83], P < 0.05) and total drainage (M/F > 40%: WMD = -7.11, 95% CI [-10.75, -3.47], P < 0.05; 40% ≥ M/F ≥ 20%: WMD = -104.72, 95% CI [-155.36, -54.08], P < 0.05; M/F < 20%: WMD = -222.00, 95% CI [-297.42, -146.58], P < 0.05). No significant differences were found for drainage on POD2 and POD3, wound complications, orthromboembolic events. In conclusion, tranexamic acid is effective and safe for blood management during HTO. Females appeared to benefit more from it, and an additional postoperative dose is suggested fora better effect.
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http://dx.doi.org/10.1111/os.13407DOI Listing
August 2022

Oral Immunization With a M Cell-Targeting Recombinant Vaccine LL-plSAM-FVpE Stimulate Protective Immunity Against in Mice.

Front Immunol 2022 7;13:918160. Epub 2022 Jul 7.

Department of Medical Laboratory, School of Clinical Medicine, Ningxia Medical University, Yinchuan, China.

There are many virulence factors of that contribute in diverse ways to gastric disease. Therefore, designing multivalent epitope vaccines against many key virulence factors virulence factors of is a promising strategy to control infection. In previous studies, we constructed a multivalent epitope vaccine FVpE against four key virulence factors of (Urease, CagA, VacA, and NAP), and oral immunization with the FVpE vaccine plus a polysaccharide adjuvant (PA) containing polysaccharide and chitosan could provide protection against infection in the Mongolian gerbil model. Oral vaccines have many advantages over injected vaccines, such as improved safety and compliance, and easier manufacturing and administration. However, the harsh gastrointestinal (GI) environment, such as gastric acid and proteolytic enzymes, limits the development of oral vaccines to some extent. Oral vaccines need a gastrointestinal delivery system with high safety, low price and promoting vaccine antigen to stimulate immune response in the gastrointestinal mucosa. Lactic acid bacteria are gastrointestinal probiotics that have unique advantages as a delivery system for oral vaccines. In this study, a M cell-targeting surface display system for named plSAM was designed to help vaccine antigens to stimulate effective immune responses in the gastrointestinal tract, and a M cell-targeting recombinant vaccine LL-plSAM-FVpE was constructed by using the surface display system plSAM. recombinant vaccine LL-plSAM-FVpE could secretively express the SAM-FVpE protein and display it on the bacterial surface. Moreover, experimental results confirmed that LL-plSAM-FVpE had an enhanced M cell-targeting property. In addition, LL-plSAM-FVpE had excellent M cell-targeting property to promote the phagocytosis and transport of the antigen SAM-FVpE by gastrointestinal M cells. More importantly, oral immunization of LL-plSAM-FVpE or SAM-FVpE plus PA can stimulate IgG and sIgA antibodies and CD4 T cell immune responses against four virulence factors of (Urease, CagA, VacA, and NAP), thus providing protective immunity against infection in mice. The M cell-targeting recombinant vaccine against various key virulence factors could be a promising vaccine candidate for controlling infection.
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http://dx.doi.org/10.3389/fimmu.2022.918160DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9336465PMC
August 2022

ACSL4 as a Potential Target and Biomarker for Anticancer: From Molecular Mechanisms to Clinical Therapeutics.

Front Pharmacol 2022 13;13:949863. Epub 2022 Jul 13.

Department of Cardiology, Chengdu Third People's Hospital/Affiliated Hospital of Southwest Jiao Tong University, Chengdu, China.

Cancer is a major public health problem around the world and the key leading cause of death in the world. It is well-known that glucolipid metabolism, immunoreaction, and growth/death pattern of cancer cells are markedly different from normal cells. Recently, acyl-CoA synthetase long-chain family 4 (ACSL4) is found be participated in the activation of long chain fatty acids metabolism, immune signaling transduction, and ferroptosis, which can be a promising potential target and biomarker for anticancer. Specifically, ACSL4 inhibits the progress of lung cancer, estrogen receptor (ER) positive breast cancer, cervical cancer and the up-regulation of ACSL4 can improve the sensitivity of cancer cells to ferroptosis by enhancing the accumulation of lipid peroxidation products and lethal reactive oxygen species (ROS). However, it is undeniable that the high expression of ACSL4 in ER negative breast cancer, hepatocellular carcinoma, colorectal cancer, and prostate cancer can also be related with tumor cell proliferation, migration, and invasion. In the present review, we provide an update on understanding the controversial roles of ACSL4 in different cancer cells.
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http://dx.doi.org/10.3389/fphar.2022.949863DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9326356PMC
July 2022

Evaluating the role of rare genetic variation in sleep duration.

Sleep Health 2022 Jul 27. Epub 2022 Jul 27.

Key Laboratory of Trace Elements and Endemic Diseases of National Health and Family Planning Commission, School of Public Health, Health Science Center, Xi'an Jiaotong University, Xi'an, China. Electronic address:

Objectives: To explore the roles of rare and high-impact variants in sleep duration.

Design: Based on the recently released UK Biobank 200k exome dataset, an exome-wide association study was conducted to detect rare variants (minor allele frequency <0.01) contributing to sleep duration. Variant annotations were performed by the software tool ANNOVAR. Gene-based burden tests of sleep duration were conducted by the SKAT R-package. After quality control, 137,047 subjects were included in this study. CAUSALdb database was used to explore the related mental traits of identified genes.

Results: We detected 730,572 variants with MAF < 1%, including 3873 frameshift variants, 3977 nonframeshift variants, 449,632 nonsynonymous variants, 1293 startloss variants, 10,254 stopgain variants, 413 stoploss variants, 261,130 synonymous variants, and 3102 variants are annotated as unknown. The burden test of exonic variants detected two exome-wide significant associations for sleep duration including TMIE at 3p21.31 (P = 0.015) and ZIC2 at 13q32.3 (P = 0.047). There are only nonsynonymous contained in TMIE; as for ZIC2, we detected 2 annotations of variants: nonsynonymous (P =2.04 × 10) and nonframeshift (P =0.85). TMIE and ZIC2 were reported to be associated with several mental traits, such as chronotype, depression, and brain natriuretic peptide in published study.

Conclusion: This study reported 2 novel candidate genes for a sleep duration, supporting the roles of rare genetic variants in the regulation of sleep duration.
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http://dx.doi.org/10.1016/j.sleh.2022.05.007DOI Listing
July 2022

Molecular mechanism of two functional protein structure changes under 2,3-butanedione-induced oxidative stress and apoptosis effects in the hepatocytes.

Int J Biol Macromol 2022 Jul 28;218:969-980. Epub 2022 Jul 28.

College of Life Science, Institute of Life Science and Green Development, Hebei University, Baoding, Hebei 071002, PR China. Electronic address:

Food security has become closely watched with the occurrence of a series of food safety incidents in recent years. The widespread adoption of 2,3-butanedione (BUT), as a food additive, is an unpreventable significant risk factor to food security. Based on this, mouse hepatocyte AML-12 cells and two functional proteins (bovine serum albumin and lysozyme) were utilized as targeted receptors to study the adverse effects of BUT at the cellular and molecular levels. Results suggested that BUT could disrupt the redox balance of AML-12 cells, reducing glutathione (GSH) activity fell to 87.18 %, which cannot offset the production of reactive oxygen species (ROS). Meanwhile, the increasement of lipid peroxidation and malondialdehyde (MDA) levels were observed. The mitochondrial membrane function was also abnormal due to the excessive accumulation of ROS and eventually leads to cell apoptosis and death. At the molecular level, the exposure of BUT could alter the skeleton and secondary structure of bovine serum albumin (BSA) and lysozyme (LYZ), and it could statically quench the intrinsic fluorescence of proteins. The combined experiments confirmed proved the potentially toxic effects of BUT accumulation on the detoxification organ, providing theoretical support for the liver diseases caused by BUT exposure, and a reference for the risk assessment of occupational exposure of BUT.
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http://dx.doi.org/10.1016/j.ijbiomac.2022.07.171DOI Listing
July 2022

Inhibition of CSPG receptor PTPσ promotes migration of newly born neuroblasts, axonal sprouting, and recovery from stroke.

Cell Rep 2022 Jul;40(4):111137

Department of Molecular Genetics, Biochemistry, and Microbiology, College of Medicine, University of Cincinnati, Cincinnati, OH 45229, USA. Electronic address:

In addition to neuroprotective strategies, neuroregenerative processes could provide targets for stroke recovery. However, the upregulation of inhibitory chondroitin sulfate proteoglycans (CSPGs) impedes innate regenerative efforts. Here, we examine the regulatory role of PTPσ (a major proteoglycan receptor) in dampening post-stroke recovery. Use of a receptor modulatory peptide (ISP) or Ptprs gene deletion leads to increased neurite outgrowth and enhanced NSCs migration upon inhibitory CSPG substrates. Post-stroke ISP treatment results in increased axonal sprouting as well as neuroblast migration deeply into the lesion scar with a transcriptional signature reflective of repair. Lastly, peptide treatment post-stroke (initiated acutely or more chronically at 7 days) results in improved behavioral recovery in both motor and cognitive functions. Therefore, we propose that CSPGs induced by stroke play a predominant role in the regulation of neural repair and that blocking CSPG signaling pathways will lead to enhanced neurorepair and functional recovery in stroke.
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http://dx.doi.org/10.1016/j.celrep.2022.111137DOI Listing
July 2022

DNA circuits compatible encoder and demultiplexer based on a single biomolecular platform with DNA strands as outputs.

Nucleic Acids Res 2022 Jul 29. Epub 2022 Jul 29.

School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.

A series of multiple logic circuits based on a single biomolecular platform is constructed to perform nonarithmetic and arithmetic functions, including 4-to-2 encoder, 1-to-2 demultiplexer, 1-to-4 demultiplexer, and multi-input OR gate. The encoder to a DNA circuit is the equivalent of a sensory receptor to a reflex arc. They all function to encode information from outside the pathway (DNA circuit or reflex arc) into a form that subsequent pathways can recognize and utilize. Current molecular encoders are based on optical or electrical signals as outputs, while DNA circuits are based on DNA strands as transmission signals. The output of existing encoders cannot be recognized by subsequent DNA circuits. It is the first time the DNA-based encoder with DNA strands as outputs can be truly applied to the DNA circuit, enabling the application of DNA circuits in non-binary biological environments. Another novel feature of the designed system is that the developed nanodevices all have a simple structure, low leakage and low crosstalk, which allows them to implement higher-level encoders and demultiplexers easily. Our work is based on the idea of complex functionality in a simple form, which will also provide a new route for developing advanced molecular logic circuits.
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http://dx.doi.org/10.1093/nar/gkac650DOI Listing
July 2022

New prediction method of horizontal principal stress of deep tight reservoirs.

Sci Rep 2022 Jul 28;12(1):12908. Epub 2022 Jul 28.

Oilfield Technology Service Company of Xinjiang Oilfield Company, PetroChina, Karamay, Xinjiang, 834000, China.

As the tight reservoir of Lucaogou Formation in Jimsar sag is characterized as large burial depth and poor physical properties, hydraulic fracturing technology is greatly needed to increase the production. Firstly, based on elastic theory, the borehole displacement formula under stress is derived, then the borehole quasi-ellipse characteristic model is proposed, on this basis, the in-situ stress prediction model based on borehole deformation is constructed. Secondly, the force analysis of diamond bit during drilling is performed, and the continuous prediction formula of elastic modulus based on weight on bit (WOB) and torque parameters is derived. Combined with these two steps, a new model of continuous in-situ stress prediction based on borehole deformation and drilling parameters is established. Compared with the values measured by the acoustic emission method in the laboratory, the deviation between the predicted in-situ stress values and those measured by the acoustic emission method is less than 4%, which meets the accuracy requirements of the fracturing in the oil field. The prediction results show that the minimum horizontal principal stress of Lucaogou Formation is 51.1-62.7 MPa, and the maximum horizontal principal stress is 58.9-69.1 MPa, respectively. The maximum horizontal principal stress direction is NW-SE direction with an angle of about 159°. The results show that the fracture direction in the study area is in accordance with the present in-situ stress direction, which is beneficial to the secondary reconstruction of natural fractures and keeps good opening property of fractures. This study provides a theoretical basis for in-situ stress prediction and compressibility evaluation of tight reservoir.
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http://dx.doi.org/10.1038/s41598-022-16954-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334574PMC
July 2022

A Cross-Sectional, Multicenter Study Examining the Validation and Adaptation of the Chinese ROWAN Foot Pain Assessment Questionnaire.

Pain Physician 2022 Aug;25(5):401-408

Department of Orthopedics, the First Medical Center, Chinese PLA General Hospital, Beijing, China.

Background: The 39-item ROwan Foot Pain Assessment Questionnaire (ROFPAQ) has affective, cognitive, and sensory dimensions to evaluate chronic foot pain. However, to date, the ROFPAQ has only been validated in English and Spanish versions. A simplified Chinese version of ROFPAQ is still not available, even though China has a large population of patients with foot pain.

Objective: This study's aim was to translate the ROFPAQ into a Chinese version and assess its reliability and validity in Chinese patients with chronic foot pain.

Study Design: A cross-sectional, multicenter descriptive study.

Setting: This study took place at the Chinese PLA General Hospital, PLA Strategic Support Force Characteristic Medical Center and Wenzhou integrated traditional Chinese and Western Medicine Hospital Affiliated to Zhejiang traditional Chinese Medicine University.

Methods: The ROFPAQ-C (Chinese) was developed by a forward/backward translation protocol and cross-cultural adaptation from the United Kingdom to China, and from English to Chinese Putonghua. A total of 194 patients from 3 centers with chronic foot pain were recruited for test-retest measures from July 2020 though September 2021.

Results: Adequate internal consistencies (Cronbach's Alpha) in 3 domains ranged from 0.875 to 0.799 for the cognitive, from 0.795 to 0.629 for the affective, and from 0.801 to 0.811 for the sensory, as well as for the total score from 0.880 to 0.815. Adequate test-retest reliability by intraclass correlation coefficient (ICC) were shown in the cognitive 0.712 (95% CI 0.636 to 0.775), the affective 0.929 (95% CI 0.906 to 0.946), the sensory 0.753 (95% CI 0.685 to 0.808), and the total score 0.932 (95% CI 0.910 to 0.948). Adequate item-total correlations were shown for the cognitive from 0.848 to 0.825, the affective from 0.918 to 0.908, and the sensory from 0.943 to 0.855.

Limitations: The original ROFPAQ with 39 items was developed from a podiatry department of the health care national service of the United Kingdom.

Conclusions: The ROFPAQ-C can be used as a valid and reliable tool for chronic foot pain in the Chinese population.
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August 2022

Cuproptosis-Related Risk Score Predicts Prognosis and Characterizes the Tumor Microenvironment in Hepatocellular Carcinoma.

Front Immunol 2022 11;13:925618. Epub 2022 Jul 11.

Department of Oncology, The Third Xiangya Hospital of Central South University, Changsha, China.

Aims: Cuproptosis is a recently identified form of programmed cell death; however, its role in hepatocellular carcinoma (HCC) remains unclear.

Methods: A set of bioinformatic tools was integrated to analyze the expression and prognostic significance of ferredoxin 1 (), the key regulator of cuproptosis. A cuproptosis-related risk score (CRRS) was developed correlation analyses, least absolute shrinkage and selection operator (LASSO) Cox regression, and multivariate Cox regression. The metabolic features, mutation signatures, and immune profile of CRRS-classified HCC patients were investigated, and the role of CRRS in therapy guidance was analyzed.

Results: was significantly downregulated in HCC, and its high expression was associated with longer survival time. HCC patients in the high-CRRS group showed a significantly lower overall survival (OS) and enriched in cancer-related pathways. Mutation analyses revealed that the high-CRRS HCC patients had a high mutational frequency of some tumor suppressors such as tumor protein P53 () and Breast-cancer susceptibility gene 1 (BRCA1)-associated protein 1 () and a low frequency of catenin beta 1 (). Besides, HCC patients with high CRRS showed an increase of protumor immune infiltrates and a high expression of immune checkpoints. Moreover, the area under the curve (AUC) values of CRRS in predicting the efficiency of sorafenib and the non-responsiveness to transcatheter arterial chemoembolization (TACE) in HCC patients reached 0.877 and 0.764, respectively.

Significance: The cuproptosis-related signature is helpful in prognostic prediction and in guiding treatment for HCC patients.
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http://dx.doi.org/10.3389/fimmu.2022.925618DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9311491PMC
July 2022

Privacy-Preserving Distributed ADMM With Event-Triggered Communication.

IEEE Trans Neural Netw Learn Syst 2022 Jul 27;PP. Epub 2022 Jul 27.

This article addresses distributed optimization problems, in which a group of agents cooperatively minimize the sum of their private objective functions via information exchanging. Building on alternating direction method of multipliers (ADMM), we propose a privacy-preserving and communication-efficient decentralized quadratically approximated ADMM algorithm, termed PC-DQM, for solving such type of problems under the scenario of limited communication. In PC-DQM, an event-triggered mechanism is designed to schedule the communication instants for reducing communication cost. Simultaneously, for privacy preservation, a Hessian matrix with perturbed noise is introduced to quadratically approximate the objective function, which results in a closed form of primal vector update and then avoids solving a subproblem at each iteration with possible high computation cost. In addition, the triggered scheme is also utilized to schedule the update of Hessian, which can also reduce computation cost. We theoretically show that PC-DQM can protect privacy but without losing accuracy. In addition, we rigorously prove that PC-DQM converges linearly to the exact optimal solution for strongly convex and smooth objective functions. Finally, numerical simulation is presented to illustrate the effectiveness and efficiency of our algorithm.
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http://dx.doi.org/10.1109/TNNLS.2022.3192346DOI Listing
July 2022

Synthesis of stack plate covalent organic framework nanotubes using a self-assembled acid as a soft template.

Chem Commun (Camb) 2022 Aug 11;58(65):9148-9151. Epub 2022 Aug 11.

Univ. Lille, CNRS, Centrale Lille, ENSCL, Univ. Artois, UMR 8181-UCCS-Unité de Catalyse et Chimie du Solide, F-59000 Lille, France.

COF-LZU1 with nanotube-like morphology has been synthesized with high crystallinity and pore volume in the presence of trimesic acid as a template. The as-synthesized COF nanotubes consist of a stack of plates with a diameter of about 100 nm with a hollow channel inside of about 20 nm.
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http://dx.doi.org/10.1039/d2cc01735kDOI Listing
August 2022

Free-Surface Velocity Measurement Using Direct Sensor Orientation-Based STIV.

Micromachines (Basel) 2022 Jul 23;13(8). Epub 2022 Jul 23.

College of Computer and Information Engineering, Hohai University, Nanjing 211100, China.

Particle image velocimetry (PIV) is a quantitative flow visualization technique, which greatly improves the ability to characterize various complex flows in laboratory and field environments. However, the deployment of reference objects or ground control points (GCPs) for velocity calibration is still a challenge for in situ free-surface velocity measurements. By combining space-time image velocimetry (STIV) with direct sensor orientation (DSO) photogrammetry, a laser distance meter (LDM)-supported photogrammetric device is designed, to realize the GCPs-free surface velocity measurement under an oblique shooting angle. The velocity calibration with DSO is based on the collinear equation, while the lens distortion, oblique shooting angle, water level variation, and water surface slope are introduced to build an imaging measurement model with explicit physical meaning for parameters. To accurately obtain the in situ position and orientations of the camera utilizing the LDM and its embedded tilt sensor, the camera's intrinsic parameters and relative position within the LDM are previously calibrated with a planar chessboard. A flume experiment is designed to evaluate the uncertainty of optical flow estimation and velocity calibration. Results show that the proposed DSO-STIV has good transferability and operability for in situ measurements. It is superior to propeller current meters and surface velocity radars in characterizing shallow free-surface flows; this is attributed to its non-intrusive, whole-field, and high-resolution features. In addition, the combined uncertainty of free-surface velocity measurement is analyzed, which provides an alternative solution for error assessment when comparing measurement failures.
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http://dx.doi.org/10.3390/mi13081167DOI Listing
July 2022

Adenylsuccinate Synthetase MoADE12 Plays Important Roles in the Development and Pathogenicity of the Rice Blast Fungus.

J Fungi (Basel) 2022 Jul 26;8(8). Epub 2022 Jul 26.

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.

Purines are basic components of nucleotides in living organisms. In this study, we identified the ortholog of adenylosuccinate synthase in by screening for growth-defective T-DNA insertional mutants. Gene replacement was performed to investigate the biological role of . mutants were adenine auxotrophs that failed to produce conidia, and showed reduced perithecia formation and pathogenicity. Moreover, the mutant was hypersensitive to Congo red and oxidants, indicating that was required for cell wall integrity and oxidative stress resistance. Transcriptomic analysis identified the underlying mechanisms and indicated that several pathogenicity-related genes were regulated in the mutant. Therefore, our data suggest that the adenylosuccinate synthase is involved in the de novo AMP biosynthesis pathway and is important for conidiation and pathogenicity in the rice blast fungus.
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http://dx.doi.org/10.3390/jof8080780DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330342PMC
July 2022

Brain Proteome-Wide Association Study Identifies Candidate Genes that Regulate Protein Abundance Associated with Post-Traumatic Stress Disorder.

Genes (Basel) 2022 Jul 27;13(8). Epub 2022 Jul 27.

Key Laboratory of Trace Elements and Endemic Diseases of National Health and Family Planning Commission, School of Public Health, Health Science Center, Xi'an Jiaotong University, Xi'an 710000, China.

Although previous genome-wide association studies (GWASs) on post-traumatic stress disorder (PTSD) have identified multiple risk loci, how these loci confer risk of PTSD remains unclear. Through the FUSION pipeline, we integrated two human brain proteome reference datasets (ROS/MAP and Banner) with the PTSD GWAS dataset, respectively, to conduct a proteome-wide association study (PWAS) analysis. Then two transcriptome reference weights (Rnaseq and Splicing) were applied to a transcriptome-wide association study (TWAS) analysis. Finally, the PWAS and TWAS results were investigated through brain imaging analysis. In the PWAS analysis, 8 and 13 candidate genes were identified in the ROS/MAP and Banner reference weight groups, respectively. Examples included ( = 3.00 × 10) and ( = 7.07 × 10). Moreover, the TWAS also detected multiple candidate genes associated with PTSD in two different reference weight groups, including ( = 3.84 × 10), ( = 5.09 × 10), and ( = 4.81 × 10). Further comparison of the PWAS and TWAS results in different populations detected the overlapping genes: ( = 4.90 × 10, = 1.23 × 10) in the total population and ( = 4.89 × 10, = 1.41 × 10) in females. Brain imaging analysis revealed several different brain imaging phenotypes associated with and genes. Our study identified multiple candidate genes associated with PTSD in the proteome and transcriptome levels, which may provide new clues to the pathogenesis of PTSD.
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http://dx.doi.org/10.3390/genes13081341DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9332745PMC
July 2022
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