Publications by authors named "Zhen Xu"

893 Publications

Comparative Aroma Profile Analysis and Development of a Sensory Aroma Lexicon of Seven Different Varieties of .

Front Nutr 2022 28;9:827825. Epub 2022 Apr 28.

School of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai, China.

() is widely planted all over the world and is rich in nutrients, which is of great benefit to the human body. However, the research on the aroma of is relatively rare, which limits the application of in deep processing, resulting in a single product and edible method of . The purpose of this study was to find out the aroma compounds contributing to the sensory properties of to promote the application of different varieties of in deep processing. Aromas of 7 species of were described and evaluated by sensory evaluation experiment. The volatile compounds in seven kinds of were detected by headspace solid-phase microextraction combined with gas chromatography-mass spectrometry (GC-MS). A total of 74 volatile compounds were found, including 23 alcohols, 5 aldehydes, 2 phenols, 1 acid, 16 esters, 7 ketones, 1 ether, 13 hydrocarbons, 1 sulfide, 1 acyl compound, and 4 heterocyclic compounds. It was also found that the sensory evaluation results of sample F, C, and E had a high correlation with the content of compound, and the correlation between sample B and sample A was also high. A lexicon for describing aroma attributes of was developed and they could be grouped into categories, such as fruity (apple-like, banana-like, cucumber-like, citrus-like and berry-like), alcoholic (whisky-like, fermented fruit-like), milky (creamy-like), floral (hyacinth-like, phoenix-like, iris-like and mint-like), sulfurous (onion-like), and musty (mud-like). This research will provide a theoretical basis for the future study of aroma and the development and application of products.
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http://dx.doi.org/10.3389/fnut.2022.827825DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9097501PMC
April 2022

Genome-wide association study reveals the genetic basis of growth trait in yellow catfish with sexual size dimorphism.

Genomics 2022 May 6;114(3):110380. Epub 2022 May 6.

College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China; Hubei Hongshan laboratory, Wuhan 430070, China. Electronic address:

Sexual size dimorphism has been widely observed in a large number of animals including fish species. Genome-wide association study (GWAS) is a powerful tool to dissect the genetic basis of complex traits, whereas the sex-differences in the genomics of animal complex traits have been ignored in the GWAS analysis. Yellow catfish (Pelteobagrus fulvidraco) is an important aquaculture fish in China with significant sexual size dimorphism. In this study, GWAS was conducted to identify candidate SNPs and genes related to body length (BL) and body weight (BW) in 125 female yellow catfish from a breeding population. In total, one BL-related SNP and three BW-related SNPs were identified to be significantly associated with the traits. Besides, one of these SNPs (Chr15:19195072) was shared in both the BW and BL traits in female yellow catfish, which was further validated in 185 male individuals and located on the exon of stat5b gene. Transgenic yellow catfish and zebrafish that expressed yellow catfish stat5b showed increased growth rate and reduction of sexual size dimorphism. These results not only reveal the genetic basis of growth trait and sexual size dimorphism in fish species, but also provide useful information for the marker-assisted breeding in yellow catfish.
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http://dx.doi.org/10.1016/j.ygeno.2022.110380DOI Listing
May 2022

Two-dimensional Topology-Seeded Graphitization for Highly Thermally Conductive Carbon Fibers.

Adv Mater 2022 May 5:e2201867. Epub 2022 May 5.

MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Key Laboratory of Adsorption and Separation Materials & Technologies of Zhejiang Province, Zhejiang University, 38 Zheda Road, Hangzhou, 310027, China.

Highly thermally conductive carbon fibers (CFs) have become an important material to meet the increasing demand for efficient heat dissipation. To date, the high thermal conductivity has been only achieved in the specific Pitch-based CFs with high crystallinity. However, obtaining high graphitic crystallinity and high thermal conductivity beyond Pitch-CFs remains a grand challenge. Here, we present a 2D topology-seeded graphitization method to mediate the topological incompatibility in graphitization by seeding 2D graphene oxide (GO) sheets into the polyacrylonitrile (PAN) precursor. Strong mechanical strength and high thermal conductivity up to 850 W/mK are simultaneously realized, which are one order of magnitude higher in conductivity than commercial PAN-based CFs. The self-oxidation and seeded graphitization effect generate large crystallite size and high orientation to far exceed those of conventional CFs. Topologically seeded graphitization, verified in experiments and simulations, allows converting the non-graphitizable into graphitizable materials by incorporating 2D seeds. This method extends the preparation of highly thermally conductive CFs, which has great potential for lightweight thermal management materials. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/adma.202201867DOI Listing
May 2022

Biomedical polymers: synthesis, properties, and applications.

Sci China Chem 2022 Apr 24:1-66. Epub 2022 Apr 24.

State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science and Laboratory of Advanced Materials, Fudan University, Shanghai, 200438 China.

Biomedical polymers have been extensively developed for promising applications in a lot of biomedical fields, such as therapeutic medicine delivery, disease detection and diagnosis, biosensing, regenerative medicine, and disease treatment. In this review, we summarize the most recent advances in the synthesis and application of biomedical polymers, and discuss the comprehensive understanding of their property-function relationship for corresponding biomedical applications. In particular, a few burgeoning bioactive polymers, such as peptide/biomembrane/microorganism/cell-based biomedical polymers, are also introduced and highlighted as the emerging biomaterials for cancer precision therapy. Furthermore, the foreseeable challenges and outlook of the development of more efficient, healthier and safer biomedical polymers are discussed. We wish this systemic and comprehensive review on highlighting frontier progress of biomedical polymers could inspire and promote new breakthrough in fundamental research and clinical translation.
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http://dx.doi.org/10.1007/s11426-022-1243-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9050484PMC
April 2022

The Influence of Growth Mindset on the Mental Health and Life Events of College Students.

Front Psychol 2022 14;13:821206. Epub 2022 Apr 14.

Department of Psychology, Neuroscience & Behaviour, McMaster University, Hamilton, ON, Canada.

Growth mindset refers to our core belief that our talents can be developed through practice, which may influence our thoughts and behaviors. Growth mindset has been studied in a variety of fields, including education, sports, and management. However, few studies have explored whether differences in individuals' growth mindsets influence college students' self-reported mental health. Using the Growth Mindset Scale, Adolescent Self-rating Life Events Checklist, and SCL-90 Scale, data was collected from 2,505 freshmen in a University in China. Findings revealed that the students within the growth mindset group scored significantly lower on "mental health issues" and "stress due to life events" than the students in the fixed mindset group. Our findings suggest that individuals with a growth mindset are less prone to mental health problems than individuals with a fixed mindset.
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http://dx.doi.org/10.3389/fpsyg.2022.821206DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9046553PMC
April 2022

Single-Cation Catalyst: Ni Cation in Monolayered CuO for CO Oxidation.

J Am Chem Soc 2022 Apr 25. Epub 2022 Apr 25.

BNLMS, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.

It is vital to differentiate catalytic properties between cationic and metallic single atoms at the atomic level. To achieve this, we fabricated well-defined cationic Ni atoms snugged in and metallic Ni atoms supported on monolayered CuO. The Ni cations are chemically inert for CO adsorption even at 70 K but highly active toward O dissociation at room temperature. The adsorbed O atoms are active to oxidize incoming CO molecules from the gas phase into CO, which follows the Eley-Rideal mechanism, in contrast to the Mars-van Krevelen mechanism on CuO-monolayer-supported metallic Ni atoms as well as our previously reported Au and Pt model catalysts. This study helps understand the chemistry of a supported single-metal cation, which is of great importance in heterogeneous catalysis.
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http://dx.doi.org/10.1021/jacs.1c12785DOI Listing
April 2022

A Sub-6 nm MnFeO-dichloroacetic acid nanocomposite modulates tumor metabolism and catabolism for reversing tumor immunosuppressive microenvironment and boosting immunotherapy.

Biomaterials 2022 May 18;284:121533. Epub 2022 Apr 18.

Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia, Brisbane, QLD, 4072, Australia; School of Chemistry and Molecular Engineering, East China Normal University, Shanghai, 200241, PR China. Electronic address:

Adenosine and lactate accumulated in tumor microenvironment are two major causes of immunosuppression, their concurrent downregulation holds promise in effective cancer immunotherapy, but remains challenging. Here, a sub-6 nm MnFeO conjugated with dichloroacetic acid (DCA) nanoparticle is developed to modulate tumor glucose metabolism and ATP catabolism for reversing the tumor immunosuppressive microenvironment. The ultrasmall MnFeO-DCA nanoparticle can efficiently enter mitochondria and supply oxygen, improving the bioactivity of DCA to regulate glucose metabolism and reduce lactate production ca. 100 times higher than free DCA itself. Moreover, this design significantly downregulates CD39 and CD73 expression than DCA or MnFeO alone, which consequently decreases the extracellular ATP catabolism. The concurrent regulation of glucose metabolism and ATP catabolism leads to increased immunostimulatory ATP level and decreased immunosuppressive adenosine and lactate levels in tumor microenvironment, eventually amplified dendritic cells maturation, enhanced cytotoxic T lymphocyte response, and improved cancer immunotherapy efficacy.
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http://dx.doi.org/10.1016/j.biomaterials.2022.121533DOI Listing
May 2022

Prevalence and Molecular Characterization of Methicillin-Resistant Staphylococci Recovered from Public Shared Bicycles in China.

Int J Environ Res Public Health 2022 04 8;19(8). Epub 2022 Apr 8.

Department of Toxicology and Sanitary Chemistry, School of Public Health, Tianjin Medical University, Qixiang Road No. 22, Tianjin 300070, China.

Millions of public shared bicycles (PSBs) have been launched in China, and PSBs are a potential reservoir of antimicrobial-resistant staphylococci. However, no national data to elucidate the dissemination, antimicrobial resistance and genotypes of staphylococci has been recovered from public shared bicycles located in different cities in China. Antimicrobial susceptibility, SCC types and sequence types of staphylococci were determined. A total of 146 staphylococci were recovered in this study, and 87% staphylococcal isolates were resistant to at least one antibiotic. In total, 29 (20%) staphylococcal isolates harbored gene, and SCC types were determined as follows: SCC type II ( = 1), IV( = 3), V ( = 4), VI ( = 1), VIII ( = 2), A/1 ( = 6), A/5 ( = 2), C/1 ( = 2), C/2 ( = 1), C/3 ( = 1), ( = 5) and Pseudo (ψ)-SCC ( = 1). Sequence types of 16 were determined, including ST10, ST17, ST59, ST60, ST65, ST130, ST184, ST262, ST283, ST337, ST360, ST454, ST567, ST820, ST878 and ST934. PSBs are a reservoir of diverse antimicrobial-resistant staphylococci, and staphylococcal species differences were observed in isolates that were recovered from public shared bicycles in the south and north of China. PSBs are a source of antimicrobial resistance and genetic diverse staphylococci.
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http://dx.doi.org/10.3390/ijerph19084492DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9027712PMC
April 2022

Genome-Wide Identification of Gene Family and Their Roles in Sarg in Response to .

Front Plant Sci 2022 1;13:849043. Epub 2022 Apr 1.

State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou, China.

Hickory () is a critical tree species of the genus from the Juglandaceae family that contains nutrient-rich nuts. Due to large-scale soil degradation, the pests and diseases of hickory are becoming more and more serious. Thaumatin-like proteins (TLPs) are vital proteins involved in the complex defense process of plant pathogens. In this study, 40 genes were identified genome-widely and phylogenetically grouped into three subfamilies. The sequence of had a conservative pattern, such as eight stable disulfide bonds, REDDD, and G-X-[GF]-X-C-X-T-[GA]-D-C-X(1,2)-G-X-(2,3)-C structure. In total, 57 -elements related to stress-responsive, light-responsive, phytohormone-responsive, and plant-responsive were discovered. Under salicylate (SA), methyl jasmonate (MeJA), and ethephon (ETH) treatments, the expressions of , and had different patterns. This is an indication that most of the genes were upregulated by SA and downregulated by MeJA. Notably, seven genes were significantly upregulated under the inoculation, especially , with an over 20-fold change. Nine genes were shown by subcellular localization analysis to be located at the plasma membrane and cytoplasm. The knowledge of the disease-resistant function of the family in hickory is promoted by these results. A foundation reference for the molecular breeding of this plant in the future is provided by our findings.
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http://dx.doi.org/10.3389/fpls.2022.849043DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9010463PMC
April 2022

Impact of Histotripsy on Development of Intrahepatic Metastases in a Rodent Liver Tumor Model.

Cancers (Basel) 2022 Mar 22;14(7). Epub 2022 Mar 22.

Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.

Histotripsy has been used for tumor ablation, through controlled, non-invasive acoustic cavitation. This is the first study to evaluate the impact of partial histotripsy ablation on immune infiltration, survival outcomes, and metastasis development, in an in vivo orthotopic, immunocompetent rat HCC model (McA-RH7777). At 7-9 days post-tumor inoculation, the tumor grew to 5-10 mm, and ~50-75% tumor volume was treated by ultrasound-guided histotripsy, by delivering 1-2 cycle histotripsy pulses at 100 Hz PRF (focal peak negative pressure P- >30 MPa), using a custom 1 MHz transducer. Complete local tumor regression was observed on MRI in 9/11 histotripsy-treated rats, with no local recurrence or metastasis up to the 12-week study end point, and only a <1 mm residual scar tissue observed on histology. In comparison, 100% of untreated control animals demonstrated local tumor progression, developed intrahepatic metastases, and were euthanized at 1-3 weeks. Survival outcomes in histotripsy-treated animals were significantly improved compared to controls (-value < 0.0001). There was evidence of potentially epithelial-to-mesenchymal transition (EMT) in control tumor and tissue healing in histotripsy-treated tumors. At 2- and 7-days post-histotripsy, increased immune infiltration of CD11b, CD8 and NK cells was observed, as compared to controls, which may have contributed to the eventual regression of the untargeted tumor region in histotripsy-treated tumors.
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http://dx.doi.org/10.3390/cancers14071612DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8996987PMC
March 2022

Teleost swim bladder, an ancient air-filled organ that elicits mucosal immune responses.

Cell Discov 2022 Apr 5;8(1):31. Epub 2022 Apr 5.

Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei, China.

The air-filled organs (AOs) of vertebrates (lungs and swim bladders) have evolved unique functions (air-breathing or buoyancy control in water) to adapt to different environments. Thus far, immune responses to microbes in AOs have been described exclusively in the lungs of tetrapods. Similar to lungs, swim bladders (SBs) represent a mucosal surface, a feature that leads us to hypothesize a role for SB in immunity. In this study, we demonstrate that secretory IgT (sIgT) is the key SB immunoglobulin (Ig) responding to the viral challenge, and the only Ig involved in viral neutralization in that organ. In support of these findings, we found that the viral load of the SB from fish devoid of sIgT was much higher than that of control fish. Interestingly, similar to the lungs in mammals, the SB represents the mucosal surface in fish with the lowest content of microbiota. Moreover, sIgT is the main Ig class found coating their surface, suggesting a key role of this Ig in the homeostasis of the SB microbiota. In addition to the well-established role of SB in buoyancy control, our findings reveal a previously unrecognized function of teleost SB in adaptive mucosal immune responses upon pathogenic challenge, as well as a previously unidentified role of sIgT in antiviral defense. Overall, our findings indicate that despite the phylogenetic distance and physiological roles of teleost SB and mammalian lungs, they both have evolved analogous mucosal immune responses against microbes which likely originated independently through a process of convergent evolution.
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http://dx.doi.org/10.1038/s41421-022-00393-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8979957PMC
April 2022

Morphology, genetic characterization and molecular phylogeny of the poorly known nematode parasite Cissophyllus leytensis Tubangui & Villaamil, 1933 (Nematoda: Ascaridida) from the Philippine sailfin lizard Hydrosaurus pustulatus (Eschscholtz, 1829) (Reptilia: Squamata).

Parasit Vectors 2022 Apr 1;15(1):116. Epub 2022 Apr 1.

Key Laboratory of Molecular Cell Biology, Ministry of Education of the People's Republic of China, Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Sciences, Hebei Normal University, Shijiazhuang, 050024, Hebei, People's Republic of China.

Background: The genus Cissophyllus (Cosmocercoidea: Kathlaniidae) is a rare group of nematodes parasitic in turtles and lizards. To date, only four species have been reported in Asia and North America. However, most of them are inadequately described. The species Cissophyllus leytensis has never been reported since it was originally described by Tubangui and Villaamil in 1933 from the Philippine sailfin lizard Hydrosaurus pustulatus (Eschscholtz, 1829) (Reptilia: Squamata). Furthermore, the systematic status of Cissophyllus/Cissophyllinae in the family Kathlaniidae of the superfamily Cosmocercoidea remains under debate.

Methods: The detailed morphology of C. leytensis was studied using light microscopy (LM) and, for the first time, scanning electron microscopy (SEM), based on newly collected specimens from the type host H. pustulatus. Six different genetic markers, including nuclear sequences [small ribosomal subunit (18S), internal transcribed spacer (ITS) and large ribosomal subunit (28S)], plus mitochondrial genes [cytochrome c oxidase subunit 1 (cox1), cytochrome c oxidase subunit 2 (cox2) and 12S small subunit ribosomal RNA gene] of C. leytensis were sequenced. Additionally, in order to test the validity of the subfamily Cissophyllinae and clarify the phylogenetic relationships of Cissophyllus and the other genera in the family Kathlaniidae, phylogenetic analyses based on 18S + 28S and ITS sequence data were performed using maximum likelihood (ML) and Bayesian inference (BI) analyses, respectively.

Results: Our observations using LM and SEM revealed some previously unreported morphological features, necessitating the redescription of this poorly known species. The presence of remarkable morphological variation in the isthmus and the position of excretory pore among different individuals was found. Molecular analysis showed no intraspecific nucleotide divergence detected in the 18S, ITS, 28S, cox2 and 12S regions among different individuals of C. leytensis, but a low level of intraspecific genetic variation was found in the cox1 (0.52%). Our phylogenetic results showed the representatives of the Cosmocercoidea divided into four large clades (Cosmocerca + Aplectana + Cosmocercoides representing the family Cosmocercidae, Cruzia representing the subfamily Cruzinae of Kathlaniidae, Falcaustra + Cissophyllus + Megalobatrachonema representing the subfamily Kathlaniinae of Kathlaniidae, and Orientatractis + Rondonia representing the family Atractidae). The genus Cissophyllus clustered together with the genus Megalobatrachonema in both the ML and BI trees using ITS sequence data, but displayed a sister relationship to the genus Falcaustra in the ML tree and to the genera Falcaustra + Megalobatrachonema in the BI tree using 18S + 28S sequence data.

Conclusions: Molecular phylogenetic results further confirmed that the family Kathlaniidae is not a monophyletic group. The subfamily Cruziinae should be moved from the hitherto-defined family Kathlaniidae and elevated as a separate family Cruziidae. The present phylogenetic results also negated the validity of the subfamily Cissophyllinae and supported the genus Cissophyllus assigned in the subfamily Kathlaniinae. Molecular analysis indicated that the morphological variation in the isthmus and position of excretory pore among different individuals should be considered as intraspecific variation. Moreover, some characters important for the specific diagnosis of C. leytensis are reported for the first time: the number of acuminate denticles (lamellae) on each lip, the chitinized pharynx with three flabellate pharyngeal plates, the presence of single medioventral precloacal papilla and the detailed morphology of caudal papillae. The present study is only the second record of C. leytensis.
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http://dx.doi.org/10.1186/s13071-022-05224-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8973900PMC
April 2022

Correction: MicroRNA-22 represses glioma development via activation of macrophage-mediated innate and adaptive immune responses.

Oncogene 2022 Apr;41(17):2526-2527

Institute of Clinical Pharmacology, Anhui Medical University, Key Laboratory of Anti-Inflammatory and Immune Medicine, Ministry of Education, Anhui Collaborative Innovation Center of Anti-Inflammatory and Immune Medicine, Hefei, China.

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http://dx.doi.org/10.1038/s41388-022-02282-1DOI Listing
April 2022

Protein Engineering of PhUGT, a Donor Promiscuous Glycosyltransferase, for the Improved Enzymatic Synthesis of Antioxidant Quercetin 3---Acetylgalactosamine.

J Agric Food Chem 2022 Apr 24;70(13):4076-4085. Epub 2022 Mar 24.

Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College (State Key Laboratory of Bioactive Substance and Function of Natural Medicines & NHC Key Laboratory of Biosynthesis of Natural Products), Beijing 100050, China.

Quercetin 3---acetylgalactosamine (Q3GalNAc), a derivative of dietary hyperoside, had never been enzymatically synthesized due to the lack of well-identified -acetylgalactosamine-transferase (GalNAc-T). Herein, PhUGT, an identified flavonoid 3--galactosyltransferase from , was demonstrated to display quercetin GalNAc-T activity, transferring a -acetylgalactosamine (GalNAc) from UDP--acetylgalactosamine (UDP-GalNAc) to the 3-OH of quercetin to form Q3GalNAc with a low conversion of 11.7% at 40 °C for 2 h. Protein engineering was thus performed, and the resultant PhUGT variant F368T got an enhanced conversion of 75.5% toward UDP-GalNAc. The enzymatically synthesized Q3GalNAc exhibited a comparable antioxidant activity with other quercetin 3--glycosides. Further studies revealed that PhUGT was a donor promiscuous glycosyltransferase (GT), recognizing seven sugar donors. This finding overturned a previous notion that PhUGT exclusively recognized UDP-galactose (UDP-Gal). The reason why PhUGT was mistaken for a UDP-Gal-specific GT was demonstrated to be a shorter reaction time, in which many quercetin 3--glycosides, except hyperoside, could not be effectively synthesized. The fact that the microbial cell factory expressing PhUGT could yield an array of Q3Gs further confirmed the donor promiscuity of PhUGT. This study laid a foundation for the scale production of Q3GalNAc and provided a potent biocatalyst capable of glycodiversifying quercetin as well.
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http://dx.doi.org/10.1021/acs.jafc.2c01029DOI Listing
April 2022

MicroRNA-10b promotes arthritis development by disrupting CD4 T cell subtypes.

Mol Ther Nucleic Acids 2022 Mar 18;27:733-750. Epub 2021 Dec 18.

Institute of Clinical Pharmacology, Anhui Medical University, Key Laboratory of Anti-Inflammatory and Immune Medicine, Ministry of Education, Anhui Collaborative Innovation Center of Anti-Inflammatory and Immune Medicine, Hefei, China.

Rheumatoid arthritis (RA) is an inflammation-involved disorder and features the disruption of CD4 T lymphocytes. Herein, we describe that microRNA-10b-5p (miR-10b) promotes RA progression by disrupting the balance between subsets of CD4 T cells. MiR-10b-deficient mice protected against collagen antibody-induced arthritis (CAIA) model. RNA sequencing results indicated that disordered genes in miR-10b CAIA model are closely associated with CD4 T cells differentiation. Moreover, miR-10b mimics promoted Th1/Th17 and suppressed Th2/Treg cells differentiation, whereas miR-10b inhibitor induced contrary effects. In addition, GATA3 and PTEN was confirmed as two targets of miR-10b, and GATA3 siRNA could increase Th1 and reduce Th2 cells meanwhile PTEN siRNA could increase Th17 and decrease Treg cells. Furthermore, miR-10b inhibitor significantly ameliorated collagen-induced arthritis (CIA) development by attenuating the dysfunctional CD4 T cell subpopulations. The present findings suggest that miR-10b could disrupt the balance of CD4 T subsets, while suppressed miR-10b could attenuate the severity of experimental arthritis, which provided us a novel mechanistic and therapeutic insight into the RA.
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http://dx.doi.org/10.1016/j.omtn.2021.12.022DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8905251PMC
March 2022

Effects of role overload, work engagement and perceived organisational support on nurses' job performance during the COVID-19 pandemic.

J Nurs Manag 2022 May 28;30(4):901-912. Epub 2022 Mar 28.

Medical College, Hebei University of Engineering, Handan, China.

Aims: We aim to study the effect of role overload, work engagement and perceived organisational support on nurses' job performance, including task performance, interpersonal facilitation and job dedication.

Background: Many nurses have suffered from role overload at work during the COVID-19 pandemic. However, the investigations of the influence mechanisms and boundary conditions through and under which role overload is associated with job performance have shown inconsistent results.

Methods: A total of 595 Chinese nurses were studied from November 2020 to February 2021. Confirmatory factor analysis, maximum likelihood estimation and bootstrapping analysis were used to test the mediating process and the moderating effect.

Results: Work engagement partly mediated the relationships of role overload with task performance (β = -.253, p < .001, 95% CI: [-.315, -.204]) and interpersonal facilitation (β = -.202, p < .001, 95% CI: [-.261, -.145]); work engagement also fully mediated the relationship between role overload and job dedication (β = -.239, p < .001, 95% CI: [-.302, -.186]). Perceived organisational support moderated the relationships of role overload with task performance, interpersonal facilitation and work dedication (β = -.171, p < .001, β = -.154, p < .001 and β = -.175, p < .001, respectively).

Conclusions: Work engagement is the linchpin linking role overload to distal outcomes of job performance. Perceived organisational support mitigates the ways in which role overload undermines job performance.

Implications For Nursing Management: Hospital administrators can minimize the effects of role overload and create a more supportive organisational environment to promote the job performance of nurses.
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http://dx.doi.org/10.1111/jonm.13598DOI Listing
May 2022

MicroRNA-22 represses glioma development via activation of macrophage-mediated innate and adaptive immune responses.

Oncogene 2022 04 12;41(17):2444-2457. Epub 2022 Mar 12.

Institute of Clinical Pharmacology, Anhui Medical University, Key Laboratory of Anti-Inflammatory and Immune Medicine, Ministry of Education, Anhui Collaborative Innovation Center of Anti-Inflammatory and Immune Medicine, Hefei, China.

Macrophage-mediated tumor cell phagocytosis and subsequent neoantigen presentation are critical for generating anti-tumor immunity. This study aimed to uncover the potential clinical value and molecular mechanisms of miRNA-22 (miR-22) in tumor cell phagocytosis via macrophages and more efficient T cell priming. We found that miR-22 expression was markedly downregulated in primary macrophages from glioma tissue samples compared to adjacent tissues. miR-22-overexpressing macrophages inhibited glioma cell proliferation and migration, respectively. miR-22 upregulation stimulated the phagocytic ability of macrophages, enhanced tumor cell phagocytosis, antigen presentation, and efficient T cell priming. Additionally, our data revealed that miR-22-overexpressing macrophages inhibited glioma formation in vivo, HDAC6 was a target, and NF-κB signaling was a pathway closely associated with miR-22 in tumor-associated macrophages (TAMs) of glioma. Our findings revealed the essential roles of miR-22 in tumor cell phagocytosis by macrophages and more efficient T cell priming, facilitating further research on phagocytic regulation to enhance the response to tumor immunotherapy.
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http://dx.doi.org/10.1038/s41388-022-02236-7DOI Listing
April 2022

Visualization of on-surface ethylene polymerization through ethylene insertion.

Science 2022 03 10;375(6585):1188-1191. Epub 2022 Mar 10.

[email protected], Synfuels China Technology Co., Ltd., Beijing 101407, China.

Polyethylene production through catalytic ethylene polymerization is one of the most common processes in the chemical industry. The popular Cossee-Arlman mechanism hypothesizes that the ethylene be directly inserted into the metal-carbon bond during chain growth, which has been awaiting microscopic and spatiotemporal experimental confirmation. Here, we report an in situ visualization of ethylene polymerization by scanning tunneling microscopy on a carburized iron single-crystal surface. We observed that ethylene polymerization proceeds on a specific triangular iron site at the boundary between two carbide domains. Without an activator, an intermediate, attributed to surface-anchored ethylidene (CHCH), serves as the chain initiator (self-initiation), which subsequently grows by ethylene insertion. Our finding provides direct experimental evidence of the ethylene polymerization pathway at the molecular level.
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http://dx.doi.org/10.1126/science.abi4407DOI Listing
March 2022

Mobile Phone Flashlight-Excited Red Afterglow Bioimaging.

Adv Mater 2022 May 25;34(18):e2201280. Epub 2022 Mar 25.

Hubei Key Lab on Organic and Polymeric Opto-Electronic Materials, Sauvage Center for Molecular Sciences, Department of Chemistry, Wuhan University, Wuhan, 430072, China.

Organic room temperature phosphorescence (RTP) materials with ultralong lifetime possess the remarkable advantage in bioimaging for elimination of background noise by characteristic time scale. However, most of RTP luminogens need to be excited by the harmful ultraviolet (UV) lamp, and exhibit green or yellow emission with shallow tissue penetration, constraining the in vivo bioimaging for further application in clinical diagnosis and pathological study. In this text, the much safer excitation process by sunlight and mobile phone flashlight is realized by organic luminogens with various electronic pull-push systems. Moreover, the bright red RTP emission with lifetime up to 344 ms is achieved by optimizing molecular geometry and electronic property. Especially, the mobile phone flashlight-excited red afterglow imaging of lymph nodes in living mice has been realized for the first time, affording a safe and conventional approach to achieve the afterglow imaging of living mice with deep issue penetration and high signal-to-noise ratios.
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http://dx.doi.org/10.1002/adma.202201280DOI Listing
May 2022

Long-distance mobile mRNA CAX3 modulates iron uptake and zinc compartmentalization.

EMBO Rep 2022 05 7;23(5):e53698. Epub 2022 Mar 7.

State Key Laboratory of Agrobiotechnology, College of Horticulture, China Agricultural University, Beijing, China.

Iron deficiency in plants can lead to excessive absorption of zinc; however, important details of this mechanism have yet to be elucidated. Here, we report that MdCAX3 mRNA is transported from the leaf to the root, and that MdCAX3 is then activated by MdCXIP1. Suppression of MdCAX3 expression leads to an increase in the root apoplastic pH, which is associated with the iron deficiency response. Notably, overexpression of MdCAX3 does not affect the apoplastic pH in a MdCXIP1 loss-of-function Malus baccata (Mb) mutant that has a deletion in the MdCXIP1 promoter. This deletion in Mb weakens MdCXIP1 expression. Co-expression of MdCAX3 and MdCXIP1 in Mb causes a decrease in the root apoplastic pH. Furthermore, suppressing MdCAX3 in Malus significantly reduces zinc vacuole compartmentalization. We also show that MdCAX3 activated by MdCXIP1 is not only involved in iron uptake, but also in regulating zinc detoxification by compartmentalizing zinc in vacuoles to avoid iron starvation-induced zinc toxicity. Thus, mobile MdCAX3 mRNA is involved in the regulation of iron and zinc homeostasis in response to iron starvation.
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http://dx.doi.org/10.15252/embr.202153698DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9066076PMC
May 2022

Exerts Maternal and Sexually Dimorphic Effects on Genomic Imprinting.

Front Cell Dev Biol 2022 2;10:784128. Epub 2022 Feb 2.

School of Life Science and Technology, ShanghaiTech University, Shanghai China.

has both maternal and zygotic functions in mouse. It maintains genomic imprinting at most known imprinted regions and controls allelic expression of the target imprinted genes in mouse embryos. The DNA methylation imprint at many imprinting control regions (ICRs) is lost when both maternal and zygotic are absent in maternal-zygotic mutant mouse embryos. Interestingly, we found that DNA methylation at a few ICRs was partially lost without maternal in heterozygous mouse embryos derived from homozygous female mice. This suggests that maternal is essential for the maintenance of DNA methylation at a small subset of imprinted regions in mouse embryos. This maternal effect of was applied to allelic expression switch as well as expression levels of the corresponding imprinted genes. It is rather surprising that DNA methylation imprint was affected differently at and imprinted regions in the female or male maternal-zygotic mutant embryos, with more significant loss of DNA methylation observed in the male mutant embryos. Loss of ZFP57 resulted in gender-specific differences in allelic expression switch and expression level changes of some imprinted genes in female or male mutant embryos. These results indicate maternal and sexually dimorphic effects of ZFP57 on genomic imprinting in mouse.
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http://dx.doi.org/10.3389/fcell.2022.784128DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8895500PMC
February 2022

Advances in Nanoliposomes for the Diagnosis and Treatment of Liver Cancer.

Int J Nanomedicine 2022 26;17:909-925. Epub 2022 Feb 26.

NHC Key Laboratory of Radiobiology (Jilin University), School of Public Health, Jilin University, Changchun, 130021, Jilin, People's Republic of China.

The mortality rate of liver cancer is gradually increasing worldwide due to the increasing risk factors such as fatty liver, diabetes, and alcoholic cirrhosis. The diagnostic methods of liver cancer include ultrasound (US), computed tomography (CT), and magnetic resonance imaging (MRI), among others. The treatment of liver cancer includes surgical resection, transplantation, ablation, and chemoembolization; however, treatment still faces multiple challenges due to its insidious development, high rate of recurrence after surgical resection, and high failure rate of transplantation. The emergence of liposomes has provided new insights into the treatment of liver cancer. Due to their excellent carrier properties and maneuverability, liposomes can be used to perform a variety of functions such as aiding in imaging diagnoses, combinatorial therapies, and integrating disease diagnosis and treatment. In this paper, we further discuss such advantages.
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http://dx.doi.org/10.2147/IJN.S349426DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8893038PMC
March 2022

The Curvilinear Relationship Between Start-Up Age and Host Growth on Sharing Accommodation Platforms.

Front Psychol 2022 9;13:811714. Epub 2022 Feb 9.

Graduate School of Technology Management, Ritsumeikan University, Osaka, Japan.

The tourism and accommodation industry has long been a fertile field for digital entrepreneurial activities. However, sharing accommodation entrepreneurs have been ignored, whether in digital entrepreneurship or the sharing economy. This empirical study explored the relationship between start-up age and host growth based on the entrepreneurship learning theory to bridge the gap. In total, 348 hosts' balanced panel data for 5 years were collected from the Airbnb platform. The results shown that (1) there was a curvilinear (inverted U-shaped) relationship between start-up age and host growth; (2) a critical primary growth strategy (product supply) significantly moderated the curvilinear relationship such that the inverted U-shaped relationship is less pronounced when the level of product supply is high. This study is helpful to understand digital entrepreneurs in the sharing accommodation and offers management suggestions for host growth.
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http://dx.doi.org/10.3389/fpsyg.2022.811714DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8864131PMC
February 2022

Kindlin-3 in Immune Cells Is Required to Suppress Prostate Cancer Tumor Growth in Mice.

Anticancer Res 2022 Mar;42(3):1217-1220

Versiti Blood Research Institute, Milwaukee, WI, U.S.A.;

Background/aim: Kindlins are essential integrin activators. Kindlin-1 and kindlin-2 are often concomitantly expressed in epithelial tumor cells and participate in regulating tumor malignancy. However, it remains unclear whether kindlin-3, the one expressed in immune cells, also plays a role in regulating tumor malignancy.

Materials And Methods: To examine the role of kindlin-3 in different immune cells in regulating solid tumor growth, a xenograft model of prostate cancer tumor growth in genetically modified kindlin-3 mice was employed.

Results: Disruption of crosstalk between kindlin-3 and integrins significantly promoted subcutaneous prostate cancer tumor growth in mice. Furthermore, deficiency of kindlin-3 in T cells and NK cells, but not myeloid cells and B cells, significantly enhanced prostate cancer tumor growth.

Conclusion: Tumor-killing leukocytes require Kindlin-3 for suppressing cancerous tumor growth, thus providing a novel anticancer mechanism.
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http://dx.doi.org/10.21873/anticanres.15588DOI Listing
March 2022

Osteocrin alleviates cardiac hypertrophy via attenuating oxidative stress.

Peptides 2022 Jun 24;152:170773. Epub 2022 Feb 24.

Intensive Care Unit, Shengli Oilfield Central Hospital, Dongying, China. Electronic address:

Osteocrin (OSTN) is a secretory peptide mainly derived from the skeletal muscles and bones. The present study aims to explore the role of OSTN in cardiac hypertrophy and its underlying mechanism. Experiments were carried out in mice receiving angiotensin (Ang) II to induce cardiac hypertrophy, and in neonatal rat cardiomyocytes (NRCMs) or human cardiac AC16 cells with Ang II-induced cardiomyocytes hypertrophy. The expression of OSTN was lower in Ang II-treated mouse heart of mice, NRCMs and AC16 cells. OSTN overexpression attenuated the hypertrophy and fibrosis of heart in mice induced by Ang II. Overexpression of OSTN inhibited hypertrophy of NRCMs and AC16 cells induced by Ang II. Increased oxidative stress was observed in the heart of mice, NRCMs and AC16 cells treated with Ang II. Overexpression of NADPH oxidase 1 (Nox1) reversed the attenuating effects of OSTN on the Ang II-induced hypertrophic cardiomyocytes. Treatment with NADPH oxidase inhibitor apocynin (APO) suppressed the hypertrophy of NRCMs and AC16 cells induced by Ang II. The above findings suggested OSTN upregulation could attenuate cardiac hypertrophy and fibrosis. The upregulation of OSTN could alleviate hypertrophy of cardiomyocytes via suppressing oxidative stress.
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http://dx.doi.org/10.1016/j.peptides.2022.170773DOI Listing
June 2022

725  nm watt-level injection-locked continuous-wave Ti:sapphire laser for a mercury optical lattice clock.

Appl Opt 2021 Dec;60(34):10750-10755

We develop a watt-level 725 nm continuous-wave tunable narrow linewidth injection-locked Ti:sapphire laser. The seed laser is frequency-locked on a Fabry-Perot cavity to narrow the linewidth and stabilize the frequency. Because the wavelength of the seed laser is located at the edge of the gain profile of the Ti:sapphire crystal, it is difficult to injection-lock the Ti:sapphire laser at 725 nm. A cavity mirror, which has a long-pass-filter coating with a sharp edge, is used in the Ti:sapphire cavity to suppress mode competition from the long wavelength side. This method effectively reduces the power requirement of the seed laser at 725 nm, and the Ti:sapphire laser can be injection-locked at higher output power. As a result, a 1 W output power with a 27% slope efficiency is achieved in the injection-locked laser, and a 210 mW UV laser is obtained in a subsequent second-harmonic generation stage. To the best of our knowledge, this is the shortest wavelength for the watt-level injection-locked continuous-wave Ti:sapphire laser. This laser will be used in a mercury optical lattice clock in the future.
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http://dx.doi.org/10.1364/AO.445703DOI Listing
December 2021

[Efficacy-related substances of blood-activating and stasis-resolving medicinals derived from Curcuma plants: a review].

Zhongguo Zhong Yao Za Zhi 2022 Jan;47(1):24-35

School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China.

Derived from Curcuma plants, Curcumae Longae Rhizoma, Curcumae Rhizoma, Wenyujin Rhizoma Concisum, and Curcumae Radix are common blood-activating and stasis-resolving medicinals in clinical practice, which are mainly used to treat amenorrhea, dysmenorrhea, chest impediment and heart pain, and rheumatic arthralgia caused by blood stasis block. According to modern research, the typical components in medicinals derived from Curcuma plants, like curcumin, demethoxycurcumin, bisdemethoxycurcumin, curdione, germacrone, curcumol, and β-elemene, have the activities of hemorheology improvement, anti-platelet aggregation, anti-thrombosis, anti-inflammation, anti-tumor, and anti-fibrosis, thereby activating blood and resolving stasis. However, due to the difference in origin, medicinal part, processing, and other aspects, the efficacy and clinical application are different. The efficacy-related substances behind the difference have not yet been systematically studied. Thus, focusing on the efficacy-related substances, this study reviewed the background, efficacy and clinical application, efficacy-related substances, and "prediction-identification-verification" research method of blood-activating and stasis-resolving medicinals derived from Curcuma plants, which is expected to lay a theoretical basis for the future research on the "similarities and differences" of such medicinals based on integrated evidence chain and to guide the scientific and rational application of them in clinical practice.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20210817.603DOI Listing
January 2022

Cortisol safeguards oogenesis by promoting follicular cell survival.

Sci China Life Sci 2022 Feb 11. Epub 2022 Feb 11.

Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, School of Life Sciences, Southwest University, Chongqing, 400715, China.

The role of glucocorticoids in oogenesis remains to be elucidated. cyp11c1 encodes the key enzyme involved in the synthesis of cortisol, the major glucocorticoid in teleosts. In our previous study, we mutated cyp11c1 in tilapia and analyzed its role in spermatogenesis. In this study, we analyzed its role in oogenesis. cyp11c1 XX tilapia showed normal ovarian morphology but poor egg quality, as indicated by the mortality of embryos before 3 d post fertilization, which could be partially rescued by the supplement of exogenous cortisol to the mother fish. Transcriptome analyses revealed reduced expression of maternal genes in the eggs of the cyp11c1 XX fish. The cyp11c1 females showed impaired vitellogenesis and arrested oogenesis due to significantly decreased serum cortisol. Further analyses revealed decreased serum E2 level and expression of amh, an important regulator of follicular cell development, and increased follicular cell apoptosis in the ovaries of cyp11c1 XX fish, which could be rescued by supplement of either exogenous cortisol or E2. Luciferase assays revealed a direct regulation of cortisol and E2 on amh transcription via GRs or ESRs. Taken together, our results demonstrate that cortisol safeguards oogenesis by promoting follicular cell survival probably via Amh signaling.
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http://dx.doi.org/10.1007/s11427-021-2051-0DOI Listing
February 2022

Corrigendum to "MK2206 enhances cisplatin-induced cytotoxicity and apoptosis in testicular cancer through Akt signaling pathway inhibition" [Transl Oncol. 2020 Jul;13(7):100769].

Transl Oncol 2022 Apr 10;18:101353. Epub 2022 Feb 10.

Department of Urology, Shandong Provincial Hospital affiliated to Shandong First Medical University, Jinan, Shandong, People's Republic of China; Department of Urology, Shandong Provincial Hospital affiliated to Shandong University, Jinan, Shandong, People's Republic of China. Electronic address:

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http://dx.doi.org/10.1016/j.tranon.2022.101353DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8844722PMC
April 2022

An efficient electrochemical immunosensor for Alpha-Fetoprotein detection based on the CoFe prussian blue analog combined PdAg hybrid nanodendrites.

Bioelectrochemistry 2022 Jan 25;145:108080. Epub 2022 Jan 25.

School of Chemistry and Chemical Engineering, Shandong University of Technology, Zibo 255049, PR China. Electronic address:

A sandwich-type electrochemical immunosensor was developed using PdAg nanodendrites modified CoFe prussian blue analog (PdAg NDs/CoFe PBA) as the signal label for the alpha-fetoprotein (AFP) detection. The novel hollow porous CoFe PBA with KCoFe phase was synthesized via self-template epitaxial growth strategy. Benefiting from the filling of K ions and the hollow porous structure, the electron transfer rate and the mass transfer rate of CoFe PBA were improved, further enhancing the catalytic capacity. In addition, the special dendritic morphology of PdAg NDs can maximize the proportion of catalytic active sites, and stable Pd-N and Ag-N bonds can further fix more immune molecules to increase the sensitivity of electrochemical immunosensors. Under optimized parameters, the fabricated immunosensor demonstrated a wide linear range detection from 100.0 fg mL to 200.0 ng mL, and a low detection limit of 18.6 fg mL. Simultaneously, the immunosensor with acceptable reproducibility, specificity, stability and exhibited satisfactory performance in human serum analysis. This work provides a new line for the detection of other tumor markers, which means that it has great application potential in immune analysis.
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http://dx.doi.org/10.1016/j.bioelechem.2022.108080DOI Listing
January 2022
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