Publications by authors named "Zhen Wu"

717 Publications

Effects of Breaking Methods on the Viscosity, Rheological Properties and Nutritional Value of Tomato Paste.

Foods 2021 Oct 9;10(10). Epub 2021 Oct 9.

College of Food Science, Southwest University, Chongqing 400715, China.

Ultrasound-assisted processing has potential application advantages as an emerging technology for preparing tomato paste. This work explored the influence of ultrasound break at 22 °C (US-Break-22) and 65 °C (US-Break-65) on the viscosity, rheological properties and nutritional values of newly prepared tomato paste, compared with traditional thermal break at 65 °C (Break-65) and 90 °C (Break-90). Results showed that the US-Break-65 paste had the largest apparent viscosity, yield stress, consistency coefficient, solid-like nature, and large amplitude oscillatory shear behavior, followed by the US-Break-22 paste, Break-90 paste, and Break-65 paste. Based on the results of the pectin-related enzymes, particle size, and serum pectin of the pastes, it was revealed that the above-mentioned properties were mainly determined by the particle size and pectin content in their serum. The level of ascorbic acid followed the order of US-Break-22 paste > US-Break-65 paste > Break-65 paste > Break-90 paste. The level of total carotenoids followed the order of US-Break-22 paste ≈ US-Break-65 paste > Break-90 paste ≈ Break-65 paste. The level of total -carotenoids followed the order of US-Break-65 paste > US-Break-22 paste > Break-90 paste > Break-65 paste. The level of phenolics and antioxidant activities followed the same order of US-Break-22 paste > US-Break-65 paste > Break-90 paste > Break-65 paste. Overall, the viscosity, rheological properties and nutritional values of the tomato pastes prepared by US-Break-65 and US-Break-22 were significantly higher than those prepared by Break-65 and Break-90. Therefore, ultrasound assisted processing can prepare high quality tomato paste and can be widely implemented in the tomato paste processing industry.
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http://dx.doi.org/10.3390/foods10102395DOI Listing
October 2021

Common Postzygotic Mutational Signatures in Healthy Adult Tissues Related to Embryonic Hypoxia.

Genomics Proteomics Bioinformatics 2021 Oct 5. Epub 2021 Oct 5.

CAS Key Laboratory of Genomic and Precision Medicine, Beijing Institute of Genomics, Chinese Academy of Sciences/China National Center for Bioinformation, Beijing 100101, China; Collaborative Innovation Center for Genetics and Development, Shanghai 200438, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

Postzygotic mutations are acquired in normal tissues throughout an individual's lifetime and hold clues for identifying mutagenic factors. We investigated postzygotic mutation spectra in healthy individuals using optimized ultra-deep exome sequencing of time-series samples from the same volunteer and samples from different individuals. In blood, sperm, and muscle cells, we resolved three common types of mutational signatures. Signatures A and B represent clock-like mutational processes, and polymorphisms of epigenetic regulation genes influence the proportion of signature B in mutation profiles. Notably, signature C, characterized by C>T transitions at GpCpN sites, tends to be a feature of diverse normal tissues. Mutations of this type are likely to occur early during embryonic development, supported by their relatively high allelic frequencies, presence in multiple tissues, and decrease in occurrence with age. Almost none of the public datasets for tumors feature this signature, except for 19.6% of samples of clear-cell renal-cell carcinoma with increased activation of the hypoxia-inducible factor 1 (HIF-1) signaling pathway. Moreover, the accumulation of signature C in the mutational profile was accelerated in a human embryonic stem cell line with drug-induced activation of HIF-1α. Thus, embryonic hypoxia may explain this novel signature across multiple normal tissues. Our study suggests that hypoxic condition in an early stage of embryonic development is a crucial factor inducing C>T transitions at GpCpN sites; and individuals' genetic background may also influence their postzygotic mutation profiles.
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http://dx.doi.org/10.1016/j.gpb.2021.09.005DOI Listing
October 2021

Correlation of genotype and phenotype in 32 patients with hereditary hemochromatosis in China.

Orphanet J Rare Dis 2021 09 28;16(1):398. Epub 2021 Sep 28.

Beijing Key Laboratory of Translational Medicine On Liver Cirrhosis , Liver Research Center, Beijing Friendship Hospital, Capital Medical University, 95 Yong-An Road, Beijing, 100050, China.

Background: Hereditary hemochromatosis (HH) is widely recognized and clinical manifestations of hemochromatosis-related (HFE-related) HH is well studied in European populations. Less is known about the clinical and laboratory characteristics of non-HFE related HH in Asian population. We aimed to explore the relationship between genotype and clinical phenotype in Chinese patients with non-HFE related hereditary hemochromatosis.

Methods: Peripheral blood samples and clinical data of patients with primary iron overload were collected from the China Registry of Genetic/Metabolic Liver Diseases. Sanger sequencing was performed in cases with primary iron overload, for 5 known HH related genes (HFE, HJV, HAMP, TFR2 and SLC40A1) and 2 novel iron homeostasis-related genes (DENND3 and SUGP2). The correlation of genotype and clinical phenotype in these patients was analyzed.

Results: Of the 32 patients with primary iron overload (23 were males and 9 were females), non-HFE variants were detected in 31 (31/32, 97%), including 8 pathogenic variants in HJV, 7 pathogenic variants in SLC40A1, 8 likely pathogenic variants in SUGP2 and 5 likely pathogenic variants in DENND3 cases. Among these 31 cases, 4 cases harbored homozygous variants, 2 cases harbored homozygous + heterozygous variants, 19 cases harbored heterozygous or combined heterozygous variants, and 6 cases harbored no any damaging variants. None of investigated cases carried damaging HAMP and TFR2 variants were found. 8 cases were classified as type 2A HH and 6 cases as type 4 HH, 10 cases as non-classical genotype, and 6 cases had no pathogenic variants from 31 cases. During the statistical analysis, we excluded one case (SLC40A1 IVS3 + 10delGTT + SUGP2 p. R639Q(homo)) with difficulty in grouping due to combined damaging variants. Cases with type 2A HH have an earlier age at diagnosis (p = 0.007). The iron index of cases in type 2A HH and type 4 HH was higher than that in other groups (p = 0.01). Arthropathy was relatively rare in all groups. None of cases with type 2A HH developed cirrhosis. Cirrhosis and diabetes are more prevalent in type 4 HH. The incidence of cirrhosis (p = 0.011), cardiac involvement (p = 0.042), diabetes (p = 0.035) and hypogonadism (p = 0.020) was statistically significant in the four groups. However, due to the limited sample size, the pairwise comparison showed no significant difference.

Conclusions: This is the first comprehensive analysis about the gene variant spectrum and phenotypic aspects of non-HFE HH in China. The results will be useful to the identification, diagnosis and management of HH in China.
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http://dx.doi.org/10.1186/s13023-021-02020-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8479922PMC
September 2021

Hyperfine-Induced Electron-Spin Dephasing in Negatively Charged Colloidal Quantum Dots: A Survey of Size Dependence.

J Phys Chem Lett 2021 Oct 24;12(39):9481-9487. Epub 2021 Sep 24.

State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai 200241, China.

The electron spin relaxation processes are complicated in semiconductor quantum dots. Different spin relaxation mechanisms may result in an increased or decreased spin relaxation rate with the size. The information on size-dependent spin dynamics helps to clarify and better understand the underlying spin relaxation processes. We investigate the size dependence of the electron spin dynamics in negatively photocharged CdSe and CdS colloidal quantum dots by time-resolved ellipticity spectroscopy. It is revealed that the electron spin dephasings of photodoped electron in zero or weak magnetic fields are dominated by the electron-nuclear hyperfine interaction for all measured samples. The hyperfine-induced electron spin dephasing time is ∼1-2 ns at room temperature and decreases with decreasing the size . In addition to a size-dependent dephasing time that is directly proportional to , our measurements also show a size-independent time component, likely due to the laser-induced nuclear spin ordering.
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http://dx.doi.org/10.1021/acs.jpclett.1c02754DOI Listing
October 2021

Visualizing Van der Waals Epitaxial Growth of 2D Heterostructures.

Adv Mater 2021 Sep 20:e2105079. Epub 2021 Sep 20.

Guangzhou Key Laboratory of Low-Dimensional Materials and Energy Storage Devices, School of Materials and Energy, Guangdong University of Technology, Guangzhou, 510006, China.

Understanding the growth mechanisms of 2D van der Waals (vdW) heterostructures is of great importance in exploring their functionalities and device applications. A custom-built system integrating physical vapor deposition and optical microscopy/Raman spectroscopy is employed to study the dynamic growth processes of 2D vdW heterostructures in situ. This allows the identification of a new growth mode with a distinctly different growth rate and morphology from those of the conventional linear growth mode. A model that explains the difference in morphologies and quantifies the growth rates of the two modes by taking the role of surface diffusion into account is proposed. A range of material combinations including CdI /WS , CdI /MoS , CdI /WSe , PbI /WS , PbI /MoS , PbI /WSe , and Bi Se /WS is systematically investigated. These findings may be generalized to the synthesis of many other 2D heterostructures with controlled morphologies and physical properties, benefiting future device applications.
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http://dx.doi.org/10.1002/adma.202105079DOI Listing
September 2021

Lack of MOF Decreases Susceptibility to Hypoxia and Promotes Multidrug Resistance in Hepatocellular Carcinoma via HIF-1α.

Front Cell Dev Biol 2021 1;9:718707. Epub 2021 Sep 1.

Shandong Provincial Key Laboratory of Animal Cell and Developmental Biology, School of Life Sciences, Advanced Medical Research Institute, Shandong University, Qingdao, China.

Hypoxia-inducible factor-1α (HIF-1α) promotes oncogenesis in hepatocellular carcinoma and is functionally linked to cell proliferation, chemoresistance, metastasis and angiogenesis. It has been confirmed that the low expression level of Males absent on the first (MOF) in hepatocellular carcinoma leads to poor prognosis of patients. However, potential regulatory mechanisms of MOF in response to hypoxia remain elusive. Our results demonstrate that MOF expression is negatively associated with HIF-1α expression in hepatocellular carcinoma tissues and in response to chloride-mimicked hypoxia in hepatocellular carcinoma cell lines. MOF regulates HIF-1α mRNA expression and also directly binds to HIF-1α to mediate HIF-1α N-terminal lysine acetylation, ubiquitination and degradation, with downstream effects on MDR1 levels. Functional inactivation of MOF enhances HIF-1α stability and causes cell tolerance to hypoxia that is insensitive to histone deacetylase inhibitor treatment. Dysfunction of MOF in hepatocellular carcinoma cells also results in chemoresistance to trichostatin A, sorafenib and 5-fluorouracil via HIF-1α. Our results suggest that MOF regulates hypoxia tolerance and drug resistance in hepatocellular carcinoma cells by modulating both HIF-1α mRNA expression and N-terminal acetylation of HIF-1α, providing molecular insight into MOF-dependent oncogenic function of hepatocellular carcinoma cells.
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http://dx.doi.org/10.3389/fcell.2021.718707DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8440882PMC
September 2021

Basic pH reversed-phase liquid chromatography (bRPLC) in combination with tip-based strong cation exchange (SCX-Tip), ReST, an efficient approach for large-scale cross-linked peptide analysis.

Anal Chim Acta 2021 Sep 7;1179:338838. Epub 2021 Jul 7.

State Key Laboratory of Genetic Engineering, Department of Biochemistry, School of Life Sciences, Fudan University, Shanghai, 200438, China. Electronic address:

Chemical cross-linking in combination with mass spectrometry (XL-MS) has emerged as a useful method for structural elucidation of proteins and protein complexes. Due to the low stoichiometry of cross-linked peptides, a specific enrichment method is always necessary prior to LC-MS/MS analysis, especially for complex samples. Currently, strong cation exchange chromatography (SCX), size exclusion chromatography (SEC), and affinity tag-based enrichment are among the widely used enrichment strategies. Herein, we present a two-dimensional strategy combining basic pH reversed-phase liquid chromatography (bRPLC) fractionation and tip-based SCX (SCX-Tip) enrichment, termed ReST, for the characterization of cross-linked peptides. We revealed the unbiased separation effects of the bRPLC in the cross-linked peptide fractionation. We optimized the enrichment conditions of SCX-Tip using well-designed cross-linked peptides. Taking advantage of the high resolution of bRPLC separation and the high enrichment efficiency of SCX-Tip, we were able to identify 43.6% more cross-linked peptides than the conventional SCX approach. The presented ReST is a simple and efficient approach for proteome-scale protein-protein interaction studies.
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http://dx.doi.org/10.1016/j.aca.2021.338838DOI Listing
September 2021

Photocatalytic Fuel Cell-Assisted Molecularly Imprinted Self-Powered Sensor: A Flexible and Sensitive Tool for Detecting Aflatoxin B1.

Anal Chem 2021 10 16;93(39):13204-13211. Epub 2021 Sep 16.

Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, PR China.

The self-powered electrochemical sensor has gained big achievements in energy and devices, but it is challenging in analytical application owing to its low energy conversion efficiency and limited selectivity caused by the plentiful interference in actual samples. Herein, a new self-powered biosensor was constructed by the integration of a photocatalytic fuel cell (PFC) with a molecular imprinting polymer (MIP) to achieve sensitive and specific detection of aflatoxin B1 (AFB). Compared with other fuel cells, the PFC owns the advantages of low cost, high energy, good stability, and friendly environment by using light as the excitation source. MoS-TiCT MXene (MoS-MX) served as the photoanode material for the first time by forming a heterojunction structure, which can enhance the photocurrent by about 3-fold and greatly improve the photoelectric conversion efficiency. Aiming at the poor selectivity of the self-powered sensor, the MIP was introduced to achieve the specific capture and separation of targets without sample pretreatment. Using the MIP and PFC as recognition and signal conversion elements, respectively, the proposed self-powered biosensor showed a wide dynamic range of 0.01-1000 ng/mL with a detection limit of 0.73 pg/mL, which opened opportunities to design more novel self-powered biosensors and promoted its application in food safety and environmental monitoring.
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http://dx.doi.org/10.1021/acs.analchem.1c02074DOI Listing
October 2021

To Punish or to Restore: How Children Evaluate Victims' Responses to Immorality.

Front Psychol 2021 13;12:696160. Epub 2021 Aug 13.

Department of Psychology, Tsinghua University, Beijing, China.

Punishment is important for deterring transgressions and maintaining cooperation, while restoration is also an effective way to resolve conflicts and undo harm. Which way do children prefer when evaluating others' reactions to immorality? Across four experiments, Chinese preschoolers (aged 4-6, = 184) evaluated victims' different reactions to possession violations (i.e., punishing the perpetrator or restoring the belongings). Children evaluated restorative reactions more positively than punitive ones. This tendency to favor restoration over punishment was influenced by the degree of punishment, with more pronounced patterns observed when punishment was harsher (Experiments 1-3). Indeed, when different degrees of punishment were directly contrasted (Experiment 4), children viewed victims who imposed milder punishment ("steal one object, remove one or two objects") more positively than those who imposed harsh punishment ("steal one object, remove three objects"). These patterns were especially manifested in preschoolers who chose restoration when being put in the victim's situation, suggesting a consistency between evaluations and behaviors. Taken together, the current study showed that children prioritize protecting the victim over harshly punishing the perpetrator, which suggests an early take on the preferred way to uphold justice.
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http://dx.doi.org/10.3389/fpsyg.2021.696160DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8414137PMC
August 2021

Photoregulated Morphological Transformation of Spiropyran Derivatives Achieving the Tunability of Interfacial Hydrophilicity.

Langmuir 2021 Sep 3;37(37):11170-11175. Epub 2021 Sep 3.

State Key Laboratory of Chemical Resource Engineering, Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, China.

Regulation of self-assembly morphology is an effective strategy to obtain advanced functional materials with expected properties. However, achieving remarkable morphological transformation by light irradiation is still a challenge. Herein, three simple spiropyran derivatives (, , and ) are constructed, achieving different degrees of morphological transformation from nanospheres to hollow tadpole-like structures (), tubular structures (), and microsheets () after ultraviolet light irradiation. Interestingly, the hollow tadpole-like structures () can further extend to Y-shaped or T-shaped tubular morphology. In the process, , , and can be isomerized from a closed-ring form (hydrophobicity) to an open-ring form (hydrophilicity) in different degrees, interacting differently with methanol solvent molecules. The formation of hollow structures or microsheets along with the isomerization of spiropyran derivatives contributes to the adjustment of the hydrophilicity of the interface. Therefore, , , and with photoregulated morphological transformation show promising applications in tunable interface materials.
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http://dx.doi.org/10.1021/acs.langmuir.1c02053DOI Listing
September 2021

Modeling of cellular response after FLASH irradiation: a quantitative analysis based on the radiolytic oxygen depletion hypothesis.

Phys Med Biol 2021 Sep 13;66(18). Epub 2021 Sep 13.

Department of Engineering Physics, Tsinghua University, Beijing 100084, People's Republic of China.

Recent studies suggest ultra-high dose rate (FLASH) irradiation can spare normal tissues from radiotoxicity, while efficiently controlling the tumor, and this is known as the 'FLASH effect'. This study performed theoretical analyses about the impact of radiolytic oxygen depletion (ROD) on the cellular responses after FLASH irradiation.Monte Carlo simulation was used to model the ROD process, determine the DNA damage, and calculate the amount of oxygen depleted () during FLASH exposure. A mathematical model was applied to analyze oxygen tension (pO) distribution in human tissues and the recovery of pOafter FLASH irradiation. DNA damage and cell survival fractions (SFs) after FLASH irradiation were calculated. The impact of initial cellular pO, FLASH pulse number, pulse interval, and radiation quality of the source particles on ROD and subsequent cellular responses were systematically evaluated.The simulated electronrange was 0.38-0.43M Gywhen pOranged from 7.5 to 160 mmHg. The calculated DNA damage and SFs show that the radioprotective effect is only evident in cells with a low pO. Different irradiation setups alter the cellular responses by modifying the pO. Single pulse delivery or multi-pulse delivery with pulse intervals shorter than 10-50 ms resulted in fewer DNA damages and higher SFs. Source particles with a low linear energy transfer (LET) have a higher capacity to deplete oxygen, and thus, lead to a more conspicuous radioprotective effect.. A systematic analysis of the cellular response following FLASH irradiation was performed to provided suggestions for future FLASH applications. The FLASH radioprotective effect due to ROD may only be observed in cells with a low pO. Single pulse delivery or multi-pulse delivery with short pulse intervals are suggested for FLASH irradiation to avoid oxygen tension recovery during pulse intervals. Source particles with low LET are preferred for their conspicuous radioprotective effects.
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http://dx.doi.org/10.1088/1361-6560/ac226dDOI Listing
September 2021

Effects of novel flavonoid-enriched yogurt on the diversity of intestinal microbiota in mice.

Braz J Microbiol 2021 Aug 27. Epub 2021 Aug 27.

State Key Laboratory for Managing Biotic and Chemical Threats To the Quality and Safety of Agro-Products, Ningbo University, 315211, Ningbo, Zhejiang, People's Republic of China.

Soy isoflavone glycoside cannot be effectively absorbed by the human intestinal tract, but probiotics with related hydrolases can transform it into aglycone to promote its absorption. In this study, a novel flavonoid-enriched yogurt was developed using an isolated β-glucosidase-producing strain (Lactiplantibacillus plantarum GY). The flavonoid aglycone-enhanced yogurt was fed to ICR mice for 21 days, and its effects were observed. The yogurt can affect the gut microbial diversity of mice, especially increasing the abundance of Parasutterella, the Bacteroidales S24-7 group, and Phascolarctobacterium in the intestinal tract of mice. Meanwhile, the ratio of Bacteroidetes/Firmicutes in the intestinal tract of mice fed with the flavonoid aglycone-enriched yogurt increased. The difference in the content of butyric acid between the L-GY + IS and the control groups was significant (P < 0.05). Therefore, milk fermentation with β-glucosidase-producing strains is a promising approach for developing flavonoid glycoside-enriched yogurt products.
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http://dx.doi.org/10.1007/s42770-021-00598-wDOI Listing
August 2021

Elevated serum lipoprotein(a) is significantly associated with angiographic progression of coronary artery disease.

Clin Cardiol 2021 Aug 25. Epub 2021 Aug 25.

Department of Cardiovascular Medicine, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.

Background: Lipoprotein(a)[Lp(a)] has been considered as an independent risk factor for coronary artery disease (CAD). The present study aimed to evaluate the association between baseline serum Lp(a) and CAD progression determined by angiographic score.

Methods: A total of 814 patients who had undergone two or more coronary computed tomography angiography at least 6 months apart were consecutively enrolled and the coronary severity was determined by the Gensini score system. Patients were stratified into two groups according to Lp(a)>300 mg/L and Lp(a) ≤ 300 mg/L or classified as "progressors" and "non-progressors" based on the Gensini score rate of change per year. The association of continuous Lp(a) and Lp(a)>300 mg/L with CAD progression were respectively assessed by logistic regression analysis. Moreover, further evaluation of those association was performed in subgroups of the study population.

Results: Patients in the "progressors" group had significant higher Lp(a) levels. Furthermore, the multivariate logistic regression analysis showed that elevated Lp(a) (odds ratio [OR]: 1.451, 95% confidence interval [CI]: 1.177-1.789, p<.001) and Lp(a)>300 mg/L (OR:1.642, 95% CI:1.018-2.649, p = .042) were positively associated with CAD progression after adjusting for confounding factors. In addition, those relation seemed to be more prominent in subjects with lower body mass index (OR: 1.880, 95% CI: 1.224-2.888, p for interaction = .060).

Conclusions: Elevated baseline serum Lp(a) is positively and independently associated with angiographic progression of CAD, particularly in participants with relatively low body mass index. Therefore, Lp(a) could be a potent risk factor for CAD progression, assisting in early risk stratification in cardiovascular patients.
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http://dx.doi.org/10.1002/clc.23718DOI Listing
August 2021

Production of a Class IIb Bacteriocin with Broad-spectrum Antimicrobial Activity in Lactiplantibacillus plantarum RUB1.

Probiotics Antimicrob Proteins 2021 Aug 23. Epub 2021 Aug 23.

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo, 315211, China.

Bacteriocins produced by lactic acid bacteria have potential use as natural food preservatives, which may alleviate current problems associated with the overuse of antibiotics and emerging multi-drug-resistant microbes. In this work, Lactiplantibacillus plantarum RUB1 was found to produce a class IIb bacteriocin with strong antibacterial activity. Except for plnXY encoding putative proteins, L. plantarum RUB1 contains most genes in five operons (plnABCD, plnGHSTUVW, plnMNOP, plnIEF, and plnRLJK) related to bacteriocin synthesis. Adding low (100 and 500 ng/mL) and medium (1 μg/mL) concentrations of PlnA to broth promoted bacteriocin production and upregulated bacteriocin gene plnA, while high concentrations (50 and 200 μg/mL) inhibited expression of these genes. Co-culturing L. plantarum RUB1 with Enterococcus hirae 1003, Enterococcus hirae LWS, Limosilactobacillus fermentum RC4, L. plantarum B6, and even Listeria monocytogenes ATCC 19111 and Staphylococcus aureus ATCC 6538 enhanced bacteriocin activity and expression of bacteriocin-related genes. This study verifies that PlnA can indeed upregulate the expression of bacteriocin genes, and also bacteriocin production can be induced by co-culture with some specific bacteria or their cell-free supernatants. Bacteriocin production by L. plantarum RUB1 is mediated by a quorum sensing mechanism, directly influenced by autoinducing peptide or specific strains. The findings provide new methods and insight into bacteriocin production mechanisms.
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http://dx.doi.org/10.1007/s12602-021-09815-2DOI Listing
August 2021

Embedding Cobalt Atom Clusters in CNT-Wired MoS Tube-in-Tube Nanostructures with Enhanced Sulfur Immobilization and Catalyzation for Li-S Batteries.

Small 2021 Oct 21;17(39):e2102710. Epub 2021 Aug 21.

School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, Jiangsu, 225002, P. R. China.

Lithium-sulfur batteries are one of the most promising next-generation energy storage systems. The efficient interconversion between sulfur/lithium polysulfides and lithium sulfide is a performance-determining factor for lithium-sulfur batteries. Herein, a novel strategy to synthesize a unique tube-in-tube CNT-wired sulfur-deficient MoS nanostructure embedding cobalt atom clusters as an efficient polysulfide regulator is successfully conducted in Li-S batteries. It is confirmed that encapsulating MWCNTs into hollow porous sulfur-deficient MoS nanotubes embedded with metal cobalt clusters not only can accelerate electron transport and confine the dissolution of lithium polysulfide by physical/chemical adsorption, but also can catalyze the kinetics of polysulfide redox reactions. Based on DFT calculations, in situ spectroscopic techniques, and various electrochemical studies, catalytic effects of CNT/MoS -Co nanocomposite in Li-S battery are deeply investigated for the first time. The CNT/MoS -Co composite cathode exhibits a very remarkable rate capability (641 mAh g at 5.0 C) and excellent cycling stability (capacity decay rate of 0.050% per cycle at 5.0 C) even at high sulfur mass loading of 3.6 mg cm . More crucially, CNT/MoS -Co tube-in-tube nanostructures present a superior specific capacity of 650 mAh g in a Li-S pouch cell at 0.2 C (4.0 mg cm ).
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http://dx.doi.org/10.1002/smll.202102710DOI Listing
October 2021

Development of Chinese mesh-type pediatric reference phantom series and application in dose assessment of Chinese undergoing computed tomography scanning.

Phys Med Biol 2021 Sep 20;66(19). Epub 2021 Sep 20.

Department of Engineering Physics, Tsinghua University, Beijing, People's Republic of China.

Pediatric patients are in a growing stage with more dividing cells than adults. Therefore, they are more sensitive to the radiation dose when undergoing computed tomography (CT) scanning. It is necessary and essential to assess the organ absorbed dose and effective dose to children. Monte Carlo simulation with computational phantoms is one of the most used methods for dose calculation in medical imaging and radiotherapy. Because of the vast change of the pediatric body with age increasing, many research groups developed series pediatric phantoms for various ages. However, most of the existing pediatric reference phantoms were developed based on Caucasian populations, which is not conformable to Chinese pediatric patients. The use of different phantoms can contribute to a difference in the dose calculation. To assess the CT dose of Chinese pediatric patients more accurately, we developed the Chinese pediatric reference phantoms series, including the 3-month (CRC3m), 1-year-old (CRC01), 5-year-old (CRC05), 10-year-old (CRC10), 15-year-old male (CRCM15), and a 15-year-old female (CRCF15) phantoms. Furthermore, we applied them to dose assessment of patients undergoing CT scanning. The GE LightSpeed 16 CT scanner was simulated and the paper presents the detailed process of phantoms development and the establishment of the CT dose database (with x-ray tube voltages of 120, 100 and 80 kVp, with collimators of 20, 10, and 5 mm width, with filters for head and body), compares for the 1-year-old results with other results based on different phantoms and analyzes the CT dose calculation results. It was found that the difference in phantoms' characteristics, organ masses and positions had a significant impact on the CT dose calculation outcomes. For the 1-year-old phantom, the dose results of organs fully covered by the x-ray beam were within 10% difference from the results of other studies. For organs partially covered and not covered by the scan range, the maximum differences came up to 84% (stomach dose, chest examinations) and 463% (gonads dose, chest examinations) respectively. The findings are helpful for the dose optimization of Chinese pediatric patients undergoing CT scanning. The developed phantoms could be applied in dose estimation of other medical modalities.
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http://dx.doi.org/10.1088/1361-6560/ac1ef1DOI Listing
September 2021

Enhanced electrochemical properties of potassium-doped lithium-rich [email protected] as cathode material for lithium-ion batteries.

J Colloid Interface Sci 2022 Jan 31;605:718-726. Epub 2021 Jul 31.

State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an 710049, PR China. Electronic address:

Lithium-rich layered oxides are believed to be the most competitive cathode materials for next-generation lithium-ion batteries (LIBs) due to their high specific capacity, but the poor cycle stability and voltage attenuation severely limit their commercial applications. In this paper, a simple method combining surface treatment via pyrolysis of polyvinyl alcohol (PVA) and potassium ions (K) doping, is designed to improve the above defects of the cobalt-free Lithium-rich material LiMnNiO (LMR). The insoluble surface byproduct LiCO and amorphous carbon nanolayer derived from the pyrolysis process of PVA alleviate the corrosion of acidic species with a favorable conductivity, while a large radius of K can enlarge the space of the lithium (Li) layer to facilitate the diffusion of Li, suppress voltage polarization, and synchronously restrain the transformation from a layered structure to a spinel-like structure. After modification, the LMR material exhibits a great initial discharge capacity of 266.0 mAh g at 0.1C, a remarkable rate capability of 159.1 mAh g at 5C and an extremely high capacity retention of 98.5% over 200 cycles at 0.5C with a small voltage drop.
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http://dx.doi.org/10.1016/j.jcis.2021.07.141DOI Listing
January 2022

Effects of music therapy on anxiety: A meta-analysis of randomized controlled trials.

Psychiatry Res 2021 Oct 25;304:114137. Epub 2021 Jul 25.

Institute of Nursing and Health, College of Nursing and Health, Henan University, Jinming Avenue, Kaifeng, Henan, 475004, China. Electronic address:

This study aims to evaluate the efficacy of music therapy on anxiety from randomized controlled trials (RCTs). The following electronic databases were utilized for selecting eligible studies that were published from inception to March 2021: PubMed, Cochrane Library, PsycINFO, Medline, Web of Science, and Embase. Standard mean difference (SMD) with 95% confidence interval (CI) values were used to evaluate the efficacy of music therapy on anxiety. Thirty-two studies with 1,924 participants were included in the meta-analysis. Music therapy lasted an average of 7.5 sessions (range, 1-24 sessions), while the average follow-up duration was 7.75 weeks (range, 1-16 weeks). Music therapy significantly reduced anxiety compared to the control group at post-intervention (SMD = -0.36, 95% CI: -0.54 to -0.17, p < 0.05), but not at follow-up (SMD = -0.23, 95% CI: -0.53 to 0.08, p >0.05). Subgroup analysis found a significantly positive effect of music therapy on anxiety in < 60 and ≥ 60 age-group (SMD = -0.31, 95% CI: -0.52 to -0.09, p < 0.05; SMD = -0.45, 95% CI: -0.85 to -0. 05, p < 0.05), developed and developing country group (SMD = -0.28, 95% CI: -0.51 to -0.06, p < 0.05; SMD = -0.49, 95% CI: -0.80 to -0.17, p < 0.05), < 12 and ≥ 12 sessions group (SMD = -0.24, 95% CI: = -0.44 to -0.03, p < 0.05; SMD = -0.59, 95% CI: -0.95 to -0.22, p < 0.05), respectively. Our study indicated that music therapy can significantly improve anxiety during treatment. But given that only eight RCTs reported the effects of music therapy at follow-up and the duration of follow-up was inconsistent, further researches are needed on the lasting effects after the intervention is discontinued.
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http://dx.doi.org/10.1016/j.psychres.2021.114137DOI Listing
October 2021

Study of comparative surgical exposure to the petroclival region using patient-specific, petroclival meningioma virtual reality models.

Neurosurg Focus 2021 08;51(2):E13

2Beijing Tiantan Hospital, Capital Medical University, Beijing, People's Republic of China; and.

Objective: Despite advancement of surgical techniques, the attachments of petroclival meningiomas near the central clival depression (CCD) remain difficult to visualize. With existing methods, the amount of tumor near the CCD that is inaccessible through various approaches cannot be compared. Tumors distort the brainstem, changing the size of the operative corridor for some but not all approaches; therefore, using cadavers with normal posterior fossae makes it impossible to compare different approaches to the tumor. The authors used virtual reality (VR) models created from the imaging data of patients to compare various surgical approaches that have otherwise been incomparable in previous studies.

Methods: CT and MRI data obtained in 15 patients with petroclival meningiomas were used to create anatomically accurate 3D VR models. For each model, various surgical approaches were performed, and the surgical freedom to 6 targets of the regions were measured. Furthermore, portions of the tumor that were visually blocked by the brainstem or bony structures were segmented and recorded as blinded volumes for comparison.

Results: The extended retrosigmoid approach generated excellent exposure of the petroclival region, but for most specimens, there was inaccessible tumor volume adjacent to the brainstem (mean 641.3 mm3, SE 161.8). In contrast, the brainstem sides of the tumors were well-visualized by all the transpetrosal approaches. The blinded volume of the tumor was largest for the retrolabyrinthine approach, and this was statistically significant compared with all other approaches (mean 2381.3 mm3, SE 185.4).

Conclusions: The authors performed a novel laboratory study by using patient CT and MRI data to generate 3D virtual models to compare surgical approaches. Since it is impossible to perform various approaches in separate surgeries in patients for comparison, VR represents a viable alternative for such comparative investigations.
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http://dx.doi.org/10.3171/2021.5.FOCUS201036DOI Listing
August 2021

DEPDC1B regulates the progression of human chordoma through UBE2T-mediated ubiquitination of BIRC5.

Cell Death Dis 2021 07 30;12(8):753. Epub 2021 Jul 30.

Department of Orthopaedic Surgery, Renji Hospital, Shanghai Jiao Tong University School of Medicine, No. 2000 Jiangyue Road, Shanghai, 200127, China.

Chordoma is a rare bone malignancy with a high rate of local recurrence and distant metastasis. Although DEP domain-containing protein 1B (DEPDC1B) is implicated in a variety of malignancies, its relationship with chordoma is unclear. In this study, the biological role and molecular mechanism of DEPDC1B in chordoma were explored. The function of DEPDC1B in chordoma cells was clarified through loss-of-function assays in vitro and in vivo. Furthermore, molecular mechanism of DEPDC1B in chordoma cells was recognized by RNA sequencing and Co-Immunoprecipitation (Co-IP) assay. The malignant behaviors of DEPDC1B knockdown chordoma cells was significantly inhibited, which was characterized by reduced proliferation, enhanced apoptosis, and hindered migration. Consistently, decreased expression of DEPDC1B suppressed tumor growth in xenograft mice. Mechanically, DEPDC1B affected the ubiquitination of baculoviral inhibitor of apoptosis repeat-containing 5 (BIRC5) through ubiquitin-conjugating enzyme E2T (UBE2T). Simultaneous downregulation of BIRC5 and DEPDC1B may exacerbate the inhibitory effects of chordoma. Moreover, BIRC5 overexpression reduced the inhibitory effects of DEPDC1B knockdown in chordoma cells. In conclusion, DEPDC1B regulates the progression of human chordoma through UBE2T-mediated ubiquitination of BIRC5, suggesting that it may be a promising candidate target with potential therapeutic value.
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http://dx.doi.org/10.1038/s41419-021-04026-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8324777PMC
July 2021

Construct interesting CuS/TiO architectures for effective removal of Cr(VI) in simulated wastewater via the strong synergistic adsorption and photocatalytic process.

Sci Total Environ 2021 Nov 15;796:148941. Epub 2021 Jul 15.

School of Chemical Engineering, School of Environmental Science and Engineering, Guangdong Provincial Key Laboratory of Petrochemical Pollution Process and Control, Guangdong University of Petrochemical Technology, Maoming 525000, Guangdong, China. Electronic address:

Most of the reduction processes for Cr (VI) removal tend to be available only at the acidic condition and the capable extent of pH is limited. Here, we developed a facile strategy for constructing CuS/TiO architectures via a facile precipitation process. The as-prepared urchin-like CuS microspheres possessed hierarchical/large porous structure and unique electrical structure, which provided a strong ability to capture the Cr(VI) ions in water. Once CuS microspheres were combined with TiO crystals (P25), a surprised high removal efficiency for Cr(VI) was obtained. With optimal molar ratio of CuS:TiO (0.72:1), 4.4 and 1.3 times in Cr(VI) removal rate were obtained with respect to pure TiO and CuS. The high removal efficiency was induced by the distinct synergistic role of strong adsorption and photocatalytic reduction originated from unique electrical structure in CuS/TiO hetero-structure. Moreover, these novel CuS/TiO architectures possess promising application for Cr effluents remediation in a wide range of pH and with co-existing anions and cations.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148941DOI Listing
November 2021

Are dietary factors involved in the association of methylation and breast cancer risk?

Br J Nutr 2021 Jul 26:1-10. Epub 2021 Jul 26.

Department of Epidemiology, School of Public Health, Harbin Medical University, Harbin, Heilongjiang Province, People's Republic of China.

DNA methylation is one of the most important epigenetic modifications in breast cancer (BC) development, and long-term dietary habits can alter DNA methylation. Cadherin-4 (CDH4, a member of the cadherin family) encodes Ca2+-dependent cell-cell adhesion glycoproteins. We conducted a case-control study (380 newly diagnosed BC and 439 cancer-free controls) to explore the relationship of CDH4 methylation in peripheral blood leukocyte DNA (PBL DNA), as well as its combined and interactive effects with dietary factors on BC risk. A case-only study (335 newly diagnosed BC) was conducted to analyse the association between CDH4 methylation in breast tissue DNA and dietary factors. CDH4 methylation was detected using quantitative methylation-specific PCR. Unconditional logistic regressions were used to analyse the association of CDH4 methylation in PBL DNA and BC risk. Cross-over analysis and unconditional logistic regression were used to calculate the combined and interactive effects between CDH4 methylation in PBL DNA and dietary factors in BC. CDH4 hypermethylation was significantly associated with increased BC risk in PBL DNA (ORadjusted (ORadj) = 2·70, (95 % CI 1·90, 3·83), P < 0·001). CDH4 hypermethylation also showed significant combined effects with the consumption of vegetables (ORadj = 4·33, (95 % CI 2·63, 7·10)), allium vegetables (ORadj = 7·00, (95 % CI 4·17, 11·77)), fish (ORadj = 7·92, (95 % CI 3·79, 16·53)), milk (ORadj = 6·30, (95 % CI 3·41, 11·66)), overnight food (ORadj = 4·63, (95 % CI 2·69, 7·99)), pork (ORadj = 5·59, (95 % CI 2·94, 10·62)) and physical activity (ORadj = 4·72, (95 % CI 2·87, 7·76)). Moreover, consuming milk was significantly related with decreased risk of CDH4 methylation (OR = 0·61, (95 % CI 0·38, 0·99)) in breast tissue. Our findings may provide direct guidance on the dietary intake for specific methylated carriers to decrease their risk for developing BC.
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http://dx.doi.org/10.1017/S0007114521002804DOI Listing
July 2021

Lacidipine Ameliorates the Endothelial Senescence and Inflammatory Injury Through CXCR7/P38/C/EBP-β Signaling Pathway.

Front Cardiovasc Med 2021 6;8:692540. Epub 2021 Jul 6.

Department of Hypertension and Vascular Disease, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.

Lacidipine, a third-generation calcium channel blocker, exerts beneficial effects on the endothelium of hypertensive patients in addition to blood pressure lowering. However, the detailed mechanism underlying Lacidipine-related endothelial protection is still elusive. Sixteen spontaneous hypertensive rats (SHRs) were randomly divided into two groups: Lacidipine-treated SHR group and saline-treated control group. Tail systolic blood pressure was monitored for four consecutive weeks. Endothelial cells (ECs) were pretreated with Lacidipine prior to being stimulated with HO, bleomycin, or Lipopolysaccharides (LPS) . Then, cell activity, migration, and senescence were measured by Cell Counting Kit-8 assay, transwell assay, and β-galactosidase staining, respectively. The fluorescent probe 2', 7'-dichlorofluorescein diacetate (DCFH-DA) was used to assess the intracellular reactive oxygen species (ROS). Related protein expression was detected by Western blotting and immunofluorescence. Our data showed that Lacidipine treatment lowered the blood pressure of SHRs accompanied by the elevation of CXCR7 expression and suppression of P38 and CCAAT/enhancer-binding protein beta (C/EBP-β) compared with the control group. experiments further demonstrated that Lacidipine increased the cell viability and function of ECs under oxidative stress, cell senescence, and inflammatory activation the CXCR7/P38/signaling pathway. Our results suggested that Lacidipine plays a protective role in EC senescence, oxidative stress, and inflammatory injury through the regulation of CXCR7/P38/C/EBP-β signaling pathway.
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http://dx.doi.org/10.3389/fcvm.2021.692540DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8290057PMC
July 2021

Development of a DNA damage model that accommodates different cellular oxygen concentrations and radiation qualities.

Med Phys 2021 Sep 12;48(9):5511-5521. Epub 2021 Aug 12.

Department of Engineering Physics, Tsinghua University, Beijing, China.

Purpose: Research regarding cellular responses at different oxygen concentrations (OCs) is of immense interest within the field of radiobiology. Therefore, this study aimed to develop a mechanistic model to analyze cellular responses at different OCs.

Methods: A DNA damage model (the different cell oxygen level DNA damage [DICOLDD] model) that examines the oxygen effect was developed based on the oxygen fixation hypothesis, which states that dissolved oxygen can modify the reaction kinetics of DNA-derived radicals generated by ionizing radiation. The generation of DNA-derived radicals was simulated using the Monte Carlo method. The decay of DNA-derived radicals due to the competing processes of chemical repair, oxygen fixation, and intrinsic damaging was described using differential equations. The DICOLDD model was fitted to the previous experimental data obtained under different irradiation configurations and validated by calculating the yields of DNA double-strand breaks (DSBs) after exposure to Cs as well as cell survival fractions (SFs) using a mechanistic model of cellular survival. Moreover, we used the DICOLDD model to calculate DNA DSB damage yields after irradiation with 0.5-50 MeV protons.

Results: Generally, DSB yields calculated after exposure to Cs at different OCs correspond to statistical uncertainties of previous experimental results. Calculated SFs of CHO and V79 cells exposed to photons, protons, and alpha particles at different OCs generally concur with those obtained in previous studies. Our results demonstrated that the variation in DSB yields was less than 10% when the cellular OC decreased from 21% to 5%. Additionally, DSB yields changed drastically when OC dropped below 1%.

Conclusions: We developed a DNA damage model to evaluate the oxygen effect and provide evidence that a reaction-kinetic model of DNA-derived radicals induced by ionizing radiation suffices to explain the observed oxygen effects. Therefore, the DICOLDD model is a powerful tool for the analysis of cellular responses at different OCs after exposure to different types of radiation.
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http://dx.doi.org/10.1002/mp.15111DOI Listing
September 2021

Substitutions at Loop Regions of TMUV E Protein Domain III Differentially Impair Viral Entry and Assembly.

Front Microbiol 2021 28;12:688172. Epub 2021 Jun 28.

Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.

Flavivirus envelope protein (E) plays an important role in cellular infection, especially in virulence and antigenicity. E domain III of Tembusu virus (TMUV) is highly conserved among flaviviruses and contains four loop regions. However, the functions of the loop regions of TMUV E domain III in the viral life cycle have not yet been discovered. In this study, using a reverse genetics system, we performed site-directed mutagenesis on loops I, II, III, and IV of TMUV E domain III. Mutant 6 (S388A.G389A.K390A) showed better proliferation than the wild-type virus, while mutants 1-5 exhibited decreased infectivity, as determined by immunofluorescence assay (IFA). Based on a TMUV replicon system, the mutations exhibited no apparent effect on TMUV RNA replication. Subcellular fractionation assays and packaging system assays indicated that mutations in loops II-IV (T332A, T332S, S365A.S366A.T367A, and S388A.G389A.K390A, respectively) disrupted virion assembly. Moreover, loops I-IV played an important role in virus binding and entry, while mutant 6 (S388A.G389A.K390A) exhibited robust activity in virus entry. Taken together, our findings indicated the critical role of the loop regions in TMUV E domain III in the virus entry and assembly process.
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http://dx.doi.org/10.3389/fmicb.2021.688172DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8273266PMC
June 2021

Comparative Study of the Adverse Events Associated With Adjuvant Use of Dexmedetomidine and Clonidine in Local Anesthesia.

Front Med (Lausanne) 2021 24;8:602966. Epub 2021 Jun 24.

Department of Anesthesiology, The Fourth Hospital of Shijiazhuang, Shijiazhuang, China.

Although clonidine and dexmedetomidine are used as alpha-2 agonists to improve the quality and duration of blockade induced by local anesthetics, no study has been reported to compare their associated adverse events in local anesthesia. The aim of this study is to compare the adverse events associated with the adjuvant use of dexmedetomidine and clonidine in local anesthesia. A comprehensive search was performed to retrieve any reported adverse event associated with adjuvant use of dexmedetomidine and clonidine in local anesthesia from published literature up to 1 July 2020. Assessment of the quality of included studies was performed by the Jadad score. A comparison of any reported adverse event was made between interventions by pooling data from studies using a direct meta-analysis technique. Dichotomous outcomes were summarized as risk ratios. The review was performed according to PRISMA guideline. From 121 articles retrieved from the search finally 14 articles including 1,120 patients had eligibility criteria for including in the meta-analysis. No significant difference was observed between bradycardia/hypotension (OR = 1.17; 95 % CI = 0.66-2.10; = 0.580; = 53.78 %, = 0.027), nausea/vomiting (OR = 0.91; 95% CI = 0.59-1.42; = 0.706; = 0.0 %, = 0.940) dizziness/headache (OR = 1.10; 95% CI = 0.44-2.75; = 0.831; = 0.0 %, = 0.882) shivering (OR = 0.95 % CI = 0.50-1.66; = 0.831; = 0.0 %, = 0.920) and dry mouth (OR = 1.00; 95 % CI = 0.50-1.96; = 0.996; = 0.0%, = 0.900). No significant difference was observed in subgroup comparison of adverse events in the intravenous or local adjuvant use of the study drugs ( > 0.05). There is no difference in adverse events associated with the intravenous or local adjuvant use of dexmedetomidine and clonidine in local anesthesia.
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http://dx.doi.org/10.3389/fmed.2021.602966DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8264190PMC
June 2021

The key amino acids of E protein involved in early flavivirus infection: viral entry.

Virol J 2021 07 3;18(1):136. Epub 2021 Jul 3.

Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang District, Chengdu, 611130, Sichuan, China.

Flaviviruses are enveloped viruses that infect multiple hosts. Envelope proteins are the outermost proteins in the structure of flaviviruses and mediate viral infection. Studies indicate that flaviviruses mainly use envelope proteins to bind to cell attachment receptors and endocytic receptors for the entry step. Here, we present current findings regarding key envelope protein amino acids that participate in the flavivirus early infection process. Among these sites, most are located in special positions of the protein structure, such as the α-helix in the stem region and the hinge region between domains I and II, motifs that potentially affect the interaction between different domains. Some of these sites are located in positions involved in conformational changes in envelope proteins. In summary, we summarize and discuss the key envelope protein residues that affect the entry process of flaviviruses, including the process of their discovery and the mechanisms that affect early infection.
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http://dx.doi.org/10.1186/s12985-021-01611-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8254458PMC
July 2021

Source and Health Risk Assessment of Heavy Metals in Soil-Ginger System in the Jing River Basin of Shandong Province, North China.

Int J Environ Res Public Health 2021 06 23;18(13). Epub 2021 Jun 23.

College of Earth Science and Engineering, Shandong University of Science and Technology, Qingdao 266590, China.

This study investigated the characteristics and sources of heavy metals in a soil-ginger system and assessed their health risks. To this end, 321 topsoil samples and eight soil samples from a soil profile, and 18 ginger samples with root-soil were collected from a ginger-planting area in the Jing River Basin. The average concentration of heavy metals in the topsoil followed the order: Cr > Zn > Pb > Ni > Cu > As > Cd > Hg. In the soil profile, at depths greater than 80 cm, the contents of Cr, Ni, and Zn tended to increase with depth, which may be related to the parent materials, whereas As and Cu contents showed little change. In contrast, Pb content decreased sharply from top to bottom, which may be attributable to external environmental and anthropogenic factors. Multivariate statistical analysis showed that Cr, Ni, Cu, Zn, and Cd contents in soil are affected by natural sources, Pb and As contents are significantly affected by human activities, and Hg content is affected by farmland irrigation. Combined results of the single pollution index (), geo-accumulation index (), and potential ecological risk assessment ( and ) suggest that soil in the study area is generally not polluted by heavy metals. In ginger, Zn content was the highest (2.36 mg/kg) and Hg content was the lowest (0.0015 mg/kg). Based on the bioconcentration factor, Cd and Zn have high potential for enrichment in ginger. With reference to the limit of heavy metals in tubers, Cr content in ginger exceeds the standard in the study area. Although Cr does not accumulate in ginger, Cr enrichment in soil significantly increases the risk of excessive Cr content in ginger.
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http://dx.doi.org/10.3390/ijerph18136749DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8268833PMC
June 2021

Ultrasensitive photoelectrochemical platform with micro-emulsion-based p-type hollow silver iodide enabled by low solubility product () for HS sensing.

Nanotechnology 2021 Jul 19;32(41). Epub 2021 Jul 19.

Division of Engineering and Applied Science, California Institute of Technology, Pasadena, CA 91125, United States of America.

Since visible-light (VL) accounting for massive solar radiation energy, a large amount of attention has been paid to the development of highly efficient visible-light-driven (VLD) semiconductor materials. However, despite recent efforts to construct VL active material, hollow structure-based silver iodide (AgI) with appropriate band gap and a large surface area are limited because of lack of a proper synthesis method. Herein, hollow AgI with p-type semiconductor behavior is constructed on the basis of micro-emulsion strategy, which enables admirable cathode photoelectrochemical (PEC) response. The as-prepared hollow AgI is applied to fabricate the PEC sensing platform and reveals a low limit of detection of 0.04 fM and a wide dynamic range up to 5 orders of magnitude toward HS. The PEC sensing mechanism is supposed to the 'signal-off' pattern on account of the ultralow solubility product () of AgS, derived from the precipitation reaction due to the high affinity between sulfide ion and Ag. Besides, the hollow structure of AgI provides sufficient surface area forproducing AgS that serves as recombination center of carrier, thus causing the efficient quenching of photocurrent signals. This work broadens the horizon of structuring VLD semiconductor nanomaterials and-based HS sensing.
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http://dx.doi.org/10.1088/1361-6528/ac1094DOI Listing
July 2021

New Nanocarrier System for Liposomes Coated with S-Layer Protein to Improve Leu-Gln-Pro-Glu Absorption through the Intestinal Epithelium.

J Agric Food Chem 2021 Jul 30;69(27):7593-7602. Epub 2021 Jun 30.

Department of Food Science and Technology, School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing 210023, Jiangsu, China.

The present study describes the development of a novel liposome nanocarrier system. The liposome was coated with CICC 6074 S-layer protein (SLP) to improve the intestinal absorption of the cholesterol-lowering peptide Leu-Gln-Pro-Glu (LQPE). The SLP-coated liposomes were prepared and characterized with morphology, particle size, zeta potential, membrane stability, Fourier transform infrared spectroscopy, and dual-channel surface plasma resonance. The results showed that SLP could successfully self-assemble on liposomes. Then, LQPE liposomes and SLP-coated LQPE liposomes (SLP-L-LQPE) were prepared. SLP-L-LQPE not only showed better sustained release properties and gastrointestinal tolerance in vitro but also increased the retention time in mice intestine. Transepithelial transport experiment indicates that the transshipment of LQPE increased significantly after being embedded by liposomes and coated with SLP. The research provides a theoretical basis for the study of SLP-coated liposomes and a potential drug delivery system for improving the intestinal absorption of peptides.
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http://dx.doi.org/10.1021/acs.jafc.1c01498DOI Listing
July 2021
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