Publications by authors named "Zhen Meng"

124 Publications

Establishing a Stable Anode-Electrolyte Interface in Mg Batteries by Electrolyte Additive.

ACS Appl Mater Interfaces 2021 Jul 6;13(28):33123-33132. Epub 2021 Jul 6.

Helmholtz Institute Ulm (HIU) Electrochemical Energy Storage, Helmholtzstraße 11, D-89081 Ulm, Germany.

Simple magnesium salts with high electrochemical and chemical stability and adequate ionic conductivity represent a new-generation electrolyte for magnesium (Mg) batteries. Similar to other Mg electrolytes, the simple-salt electrolyte also suffers from high charge-transfer resistance on the Mg surface due to the adsorbed species in the solution. In the current study, we built a model Mg cell system with the Mg[B(hfip)]/DME electrolyte and Chevrel phase MoS cathode, to demonstrate the effect of such anode-electrolyte interfacial properties on the full-cell performance. It was found that the cell required additional activation cycles to achieve its maximal capacity. The activation process is mainly attributed to the conditioning of the anode-electrolyte interface, which could be boosted by introducing an additive amount of Mg(BH) to the Mg[B(hfip)]/DME electrolyte. Electrochemical and spectroscopic analyses revealed that the Mg(BH) additive helps to remove the native oxide layer and promotes the formation of a solid electrolyte interphase layer on Mg. As a result, the full cell with the additive-containing electrolyte delivered a stable capacity from the second cycle onward. Further battery tests showed a reversible cycling for 600 cycles and an excellent rate capability, indicating good compatibility of the Mg(BH) additive. The current study not only provides fundamental insights into the interfacial phenomena in Mg batteries but also highlights the facile tunability of the simple-salt Mg electrolytes.
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http://dx.doi.org/10.1021/acsami.1c08476DOI Listing
July 2021

[Research progress in biosynthesis of podophyllotoxin and its derivatives].

Sheng Wu Gong Cheng Xue Bao 2021 Jun;37(6):2026-2038

State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, Shandong, China.

Podophyllotoxin (PTOX) is an aryl-tetralin lignan of plant origin found in some species of Podophyllum such as Dysosma versipellis, Diphylleia sinensis, and Sinopodophyllum hexandrum. Etoposide and teniposide are produced semisynthetically from PTOX and used clinically to treat several forms of cancer. As a typical representative of new drug discovery from natural products, the production of PTOX solely depends on extraction from plants, resulting in severe contradiction between supply and demand. With the advantages of unconstrained resources and eco-friendly reaction conditions, biosynthesis method has become a trend in the production of PTOX and its derivatives. In this review, we summarize the research progress of PTOX biosynthesis in plants and expound the functions of the key enzymes as well as their subcellular location. The synthetic biology for production of PTOX intermediates in a tobacco chassis is also introduced. Finally, the heterologous expression and biotransformation of PTOX in microorganisms is summarized, which sets the foundation for the efficient microbial production of PTOX using cell factories.
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http://dx.doi.org/10.13345/j.cjb.210258DOI Listing
June 2021

[Determination of trace -amanitin in urine of mushroom poisoning patient by online solid phase extraction-liquid chromatography-tandem mass spectrometry].

Se Pu 2020 Nov;38(11):1281-1287

Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China.

An analytical method was established for the determination of trace -amanitin in the urine of patients suffering from mushroom poisoning by online solid phase extraction-liquid chromatography-tandem mass spectrometry (online SPE-LC-MS/MS). The sample was protein precipitated with formic acid acidified acetonitrile-methanol (5:1, v/v). Reversed-phase liquid-liquid microextraction was used to remove the organic solvent from the sample extract. The toxin was purified by online SPE using an ODS micro column (5 mm×2.1 mm, 5 μm), and separated on an XBridge BEH C column (150 mm×3.0 mm, 2.5 μm). Finally, the toxin was measured by MS/MS in the negative electrospray ionization (ESI) mode. Multiple reaction monitoring (MRM) was used, and the conditions were 917.4>205.1 (quantitative ion transition) and 917.4>257.1. Collision energy for both transitions was 55 eV. A fast valve-switching technique with a quantitative loop was used as an interface between the online SPE and LC-MS/MS modules. The two modules were independent, neither the mobile phase nor the pressure would interfere with each other, thus ensuring the stability of the system. Precise purification by the online system could effectively eliminate the matrix effects in the subsequent MS detection. Weak matrix suppression effects were found, with results of 88.7%-96.5%. The linear range of -amanitin in urine was 0.1-50 μg/L with a correlation coefficient () of 0.9983. The limit of detection (LOD) and limit of quantification (LOQ) in the sample matrix were 0.03 μg/L and 0.1 μg/L, respectively. The average recoveries at three spiked levels (0.1, 2.0 and 20 μg/L) were 84.3%-91.7% with relative standard deviations (RSDs) of 3.8%-7.2%. The accuracy and precision were evaluated using quality control samples with toxin contents of 0.1 μg/L (LOQ), 0.2 μg/L (2-fold LOQ), 2.0 μg/L (medium level), and 20 μg/L (high level). The calculated average intra-day accuracy was 85.1%-96.0% with the precision of 4.1%-7.8%. The inter-day accuracy was 82.9%-94.8% with the precision of 5.0%-9.5%. The specificity of the method was verified by negative samples derived from patients who suffered only gastroenteritis poisoning, without hepatotoxic symptoms. -Amanitin was found in urine samples from nine mushroom poisoning patients with hepatotoxic symptoms. The sampling time ranged from 19 h to 92 h. The toxin contents were 0.11-53.1 μg/L. For patients with a high intake of poisonous mushrooms, the toxin content was 53.1 μg/L in a patient's urine sampled 19 h after accidental consumption and 0.19 μg/L in another patient's urine sampled 92 h after poisoning. The content of -amanitin was only 0.53 μg/L in the urine sample obtained 23 h after consumption for a patient with low intake and 0.11 μg/L in the urine sampled from another patient 40 h after poisoning. Amatoxins can metabolize rapidly in vivo. The laboratory identification of amatoxin poisoning requires a method for trace-level analysis in the biological matrix. It is proved that this method is simple, accurate and sensitive by the application to the analysis of actual samples. The protein precipitation and reversed-phase liquid-liquid microextraction steps are fast and simple. Hence, they can be used as a rapid and effective pre-treatment method for online SPE-LC-MS/MS analysis of water-soluble toxins in biomaterial matrix. Highly sensitive analysis of -amanitin in urine can be obtained using a precise purification technology via online SPE in this study. The problem of qualitative confirmation of the toxin at trace levels (0.03 μg/L) after poisoning can be solved. The laboratory identification time for amatoxin poisoning in some patients exceeds 90 h. The developed analytical method at trace level (0.1 μg/L of LOQ) can provide reliable technical support for establishing the dose-response relationship of -amanitin in vivo. It can satisfy for the determination of trace -amanitin in urine samples from patients with hepatotoxic mushroom poisoning.
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http://dx.doi.org/10.3724/SP.J.1123.2020.03010DOI Listing
November 2020

Directional-sum test for nonparametric Behrens-Fisher problem with applications to the dietary intervention trial.

Stat Methods Med Res 2021 Jun 17:9622802211002864. Epub 2021 Jun 17.

School of Statistics, Capital University of Economics and Business, Beijing, China.

For a nonparametric Behrens-Fisher problem, a directional-sum test is proposed based on division-combination strategy. A one-layer wild bootstrap procedure is given to calculate its statistical significance. We conduct simulation studies with data generated from lognormal, and Laplace distributions to show that the proposed test can control the type I error rates properly and is more powerful than the existing rank-sum and maximum-type tests under most of the considered scenarios. Applications to the dietary intervention trial further show the performance of the proposed test.
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http://dx.doi.org/10.1177/09622802211002864DOI Listing
June 2021

Effects of curcumin on the bioavailability of dioxin-like pollutants in rats.

Sci Rep 2021 Jun 14;11(1):12461. Epub 2021 Jun 14.

Zhejiang Provincial Center for Disease Control and Prevention, Binsheng Road 3399, Hangzhou, 310051, China.

The effects of curcumin on the bioavailability of polychlorinated dibenzo-p-dioxins/furans (PCDD/Fs) and dioxin-like polychlorinated biphenyls (DL-PCBs) were investigated in Sprague-Dawley rats. Tetra- and penta-chlorinated PCDFs had the lowest bioavailability and hexa-chlorinated PCDD/Fs had the highest, while there was no obvious change in that of DL-PCBs. Curcumin markedly reduced the toxic equivalent (TEQ) of PCDD/Fs in rats, illustrating the potential to competitively inhibit absorption of PCDD/Fs by the epithelial cells of the small intestine due to the similar chemical structure (diphenyl) between curcumin and PCDD/Fs. Moreover, curcumin lowered the TEQ of DL-PCBs in the liver of male rats, but not female rats. The significant decrease in the bioavailability of PCDD/Fs and DL-PCBs demonstrates the potential detoxification mechanisms of curcumin.
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http://dx.doi.org/10.1038/s41598-021-92085-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8203761PMC
June 2021

A ROC-based test for evaluating the group difference with an application to neonatal audiology screening.

Stat Med 2021 May 29. Epub 2021 May 29.

LSC, NCMIS, Academy of Mathematics and Systems Science, Chinese Academy of Sciences, Beijing, China.

This article proposes a powerful method to compare two samples. The proposed method handles comparison of data by drawing inference from ROC curve model parameters. The method estimates parameters from a linear model framework on the empirical sensitivities and specificities. The consistent ROC parameters are then used to give a more powerful test than existing methods in several situations. In addition, we present a comprehensive statistic based on the Cauchy combination, which works well in all scenarios considered in this article. We also offer an efficient one-layer wild permutation procedure to calculate the P-value of our statistic. The method is particularly useful when the underlying continuous biomarker results are non-normal. We illustrate the proposed methods in a neonatal audiology diagnostic example.
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http://dx.doi.org/10.1002/sim.9082DOI Listing
May 2021

Ectopic tooth in maxillary sinus compressing the nasolacrimal canal: A case report.

Medicine (Baltimore) 2021 May;100(18):e25514

Department of Stomatology.

Rationale: Ectopic tooth is tooth erupting out of normal anatomical position. Ectopic tooth can occur in different positions, such as maxillary sinus and nasal cavity. In this article, we present a rare case of an ectopic tooth with a dentigerous cyst in the maxillary sinus compressing the nasolacrimal canal.

Patient Concerns: An 8-year-old girl presented with a 2-month history of spontaneous lacrimation in her right eye. When she wept, more tear shed from her right eye than that from the left one. Computed tomographic (CT) imaging showed a huge low-density image containing a tooth in the maxillary sinus in her right maxilla; the right nasolacrimal canal vanished due to the compression of the ectopic tooth.

Diagnoses: Ectopic tooth with dentigerous cyst of right maxilla, and obstruction of nasolacrimal duct.

Interventions: The patient underwent nasal endoscopic maxillary sinus cystectomy.

Outcomes: The patient recovered well after cystectomy and has been symptom-free.

Lessons: The unique finding is that this is the first report about ectopic tooth compressing the nasolacrimal canal and inducing spontaneous lacrimation. Treatment: aspect: surgery under endoscope is a minimally invasive approach to ectopic tooth.
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http://dx.doi.org/10.1097/MD.0000000000025514DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8104135PMC
May 2021

Multi-walled carbon nanotube polysaccharide modified Hericium erinaceus polysaccharide as an adjuvant to extend immune responses.

Int J Biol Macromol 2021 Jul 30;182:574-582. Epub 2021 Mar 30.

Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health in Fujian Province, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China. Electronic address:

In recent years, the utilization of CS-MWCNT as targeted drug carriers has attracted considerable attention. Hericium erinaceus polysaccharide (HEP) has been reported as an immunostimulant to improve immune responses. This study was focussed on developing CS-MWCNT encapsulating HEP (CS-MWCNT-HEP). Using in mice peritoneal macrophages, we found the immune response could be effectively regulated by CS-MWCNT-HEP, promoted the expression of the MHCII, CD86, F4/80 and gp38. Moreover, the mice immunized with CS-MWCNT-HEP nanoparticles significantly extended PCV-specific IgG immune response and the levels of cytokines. The results demonstrated that CS-MWCNT-HEP may be a promising drug delivery system for immuno-enhancement.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.03.180DOI Listing
July 2021

Aldosterone alleviates lipopolysaccharide-induced acute lung injury by regulating epithelial sodium channel through PI3K/Akt/SGK1 signaling pathway.

Mol Cell Probes 2021 Jun 10;57:101709. Epub 2021 Mar 10.

Procesion Biomedical Lab, Liaocheng People's Hospital, Medical College of Liaocheng University, #67 Dongchangxi Road, Liaocheng, 252000, China. Electronic address:

Reduced alveolar fluid clearance (AFC) is a major pathological feature of acute lung injury (ALI). Epithelial sodium channel (ENaC) plays a key role in regulating the transport of Na and clearing alveolar edema fluid effectively. ENaC has been reported to be regulated by aldosterone in the distal collecting tube of the kidney. We hypothesized whether aldosterone regulated ENaC in alveolar epithelium and correspondingly played a role in ALI. In this study we found that the expression of aldosterone synthesis encoding gene, CYP11B2, and ENaC were decreased in the lung tissue of LPS-induced ALI mice. Furthermore, aldosterone alleviated ALI by increasing the expression of ENaC-α and relieving pulmonary edema. Besides, we found that aldosterone upregulated ENaC-α through PI3K/Akt/SGK1 pathway. In conclusion, our study demonstrated that aldosterone attenuated pulmonary edema by upregulating ENaC-α through the PI3K/Akt/SGK1 pathway in LPS-induced ALI, indicating that aldosterone might be a promising adjuvant drug for ALI treatment.
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http://dx.doi.org/10.1016/j.mcp.2021.101709DOI Listing
June 2021

Hybrid Deep Recurrent Neural Networks for Noise Reduction of MEMS-IMU with Static and Dynamic Conditions.

Micromachines (Basel) 2021 Feb 20;12(2). Epub 2021 Feb 20.

Institute of Microelectronics, Chinese Academy of Sciences, Beijing 100029, China.

Micro-electro-mechanical system inertial measurement unit (MEMS-IMU), a core component in many navigation systems, directly determines the accuracy of inertial navigation system; however, MEMS-IMU system is often affected by various factors such as environmental noise, electronic noise, mechanical noise and manufacturing error. These can seriously affect the application of MEMS-IMU used in different fields. Focus has been on MEMS gyro since it is an essential and, yet, complex sensor in MEMS-IMU which is very sensitive to noises and errors from the random sources. In this study, recurrent neural networks are hybridized in four different ways for noise reduction and accuracy improvement in MEMS gyro. These are two-layer homogenous recurrent networks built on long short term memory (LSTM-LSTM) and gated recurrent unit (GRU-GRU), respectively; and another two-layer but heterogeneous deep networks built on long short term memory-gated recurrent unit (LSTM-GRU) and a gated recurrent unit-long short term memory (GRU-LSTM). Practical implementation with static and dynamic experiments was carried out for a custom MEMS-IMU to validate the proposed networks, and the results show that GRU-LSTM seems to be overfitting large amount data testing for three-dimensional axis gyro in the static test. However, for -axis and -axis gyro, LSTM-GRU had the best noise reduction effect with over 90% improvement in the three axes. For -axis gyroscope, LSTM-GRU performed better than LSTM-LSTM and GRU-GRU in quantization noise and angular random walk, while LSTM-LSTM shows better improvement than both GRU-GRU and LSTM-GRU networks in terms of zero bias stability. In the dynamic experiments, the Hilbert spectrum carried out revealed that time-frequency energy of the LSTM-LSTM, GRU-GRU, and GRU-LSTM denoising are higher compared to LSTM-GRU in terms of the whole frequency domain. Similarly, Allan variance analysis also shows that LSTM-GRU has a better denoising effect than the other networks in the dynamic experiments. Overall, the experimental results demonstrate the effectiveness of deep learning algorithms in MEMS gyro noise reduction, among which LSTM-GRU network shows the best noise reduction effect and great potential for application in the MEMS gyroscope area.
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http://dx.doi.org/10.3390/mi12020214DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7923423PMC
February 2021

The value of microRNA-203 as a biomarker for the prognosis of esophageal cancer: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2020 Dec;99(50):e23599

Department of Clinical Laboratory, Liaocheng people's Hospital, Liaocheng, Shandong Province, China.

Background: Previous studies have reported that microRNA-203 has an effect on the prognosis of with esophageal cancer (EC). However, the conclusion is remains controversial. Therefore, this study will try to explore the effect of high expression of microRNA-203 on the prognosis of EC patients.

Methods: Eligible studies were searched from Google Scholar, Embase, PubMed, Medline, Web of Science, Cochrane Library, China National Knowledge Infrastructure, China Scientific Journal Database, Chinese BioMedical Database and Wanfang Database. Papers in English or Chinese published from their inception to November 2020 will be included without any restrictions. Stata 14.0 and Review Manager 5.3 software were used for data analysis. Hazard ratios (HRs) and its 95% confidence intervals (CIs) were used to assess the prognostic effect of microRNA-203 on overall survival (OS) and disease-free survival (DFS). Methodological quality for each eligible trial will be assessed by using the Newcastle-Ottawa Quality Assessment Scale (NOS).

Results: This study will provide a high-quality evidence-based medical evidence of the correlations between microRNA-203 expression and OS and DFS.

Conclusion: The findings of this meta-analysis will show the effect of high expression of microRNA-203 on the prognosis of EC patients, and may find a new prognostic marker for EC.

Inplasy Registration Number: INPLASY2020110022.
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http://dx.doi.org/10.1097/MD.0000000000023599DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7738148PMC
December 2020

Modulating Magnetism in Ferroelectric Polymer-Gated Perovskite Manganite Films with Moderate Gate Pulse Chains.

ACS Appl Mater Interfaces 2020 Dec 6;12(50):56541-56548. Epub 2020 Dec 6.

Department of Applied Physics, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China.

Most previous attempts on achieving electric-field manipulation of ferromagnetism in complex oxides, such as LaSrMnO (LSMO), are based on electrostatically induced charge carrier changes through high- dielectrics or ferroelectrics. Here, the use of a ferroelectric copolymer, polyvinylidene fluoride with trifluoroethylene [P(VDF-TrFE)], as a gate dielectric to successfully modulate the ferromagnetism of the LSMO thin film in a field-effect device geometry is demonstrated. Specifically, through the application of low-voltage pulse chains inadequate to switch the electric dipoles of the copolymer, enhanced tunability of the oxide magnetic response is obtained, compared to that induced by ferroelectric polarization. Such observations have been attributed to electric field-induced oxygen vacancy accumulation/depletion in the LSMO layer upon the application of pulse chains, which is supported by surface-sensitive-characterization techniques, including X-ray photoelectron spectroscopy and X-ray magnetic circular dichroism. These techniques not only unveil the electrochemical nature of the mechanism but also establish a direct correlation between the oxygen vacancies created and subsequent changes to the valence states of Mn ions in LSMO. These demonstrations based on the pulsing strategy can be a viable route equally applicable to other functional oxides for the construction of electric field-controlled magnetic devices.
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http://dx.doi.org/10.1021/acsami.0c14172DOI Listing
December 2020

Random Error Reduction Algorithms for MEMS Inertial Sensor Accuracy Improvement-A Review.

Micromachines (Basel) 2020 Nov 21;11(11). Epub 2020 Nov 21.

Institute of Microelectronics, Chinese Academy of Sciences, Beijing 100029, China.

Research and industrial studies have indicated that small size, low cost, high precision, and ease of integration are vital features that characterize microelectromechanical systems (MEMS) inertial sensors for mass production and diverse applications. In recent times, sensors like MEMS accelerometers and MEMS gyroscopes have been sought in an increased application range such as medical devices for health care to defense and military weapons. An important limitation of MEMS inertial sensors is repeatedly documented as the ease of being influenced by environmental noise from random sources, along with mechanical and electronic artifacts in the underlying systems, and other random noise. Thus, random error processing is essential for proper elimination of artifact signals and improvement of the accuracy and reliability from such sensors. In this paper, a systematic review is carried out by investigating different random error signal processing models that have been recently developed for MEMS inertial sensor precision improvement. For this purpose, an in-depth literature search was performed on several databases viz., Web of Science, IEEE Xplore, Science Direct, and Association for Computing Machinery Digital Library. Forty-nine representative papers that focused on the processing of signals from MEMS accelerometers, MEMS gyroscopes, and MEMS inertial measuring units, published in journal or conference formats, and indexed on the databases within the last 10 years, were downloaded and carefully reviewed. From this literature overview, 30 mainstream algorithms were extracted and categorized into seven groups, which were analyzed to present the contributions, strengths, and weaknesses of the literature. Additionally, a summary of the models developed in the studies was presented, along with their working principles viz., application domain, and the conclusions made in the studies. Finally, the development trend of MEMS inertial sensor technology and its application prospects were presented.
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http://dx.doi.org/10.3390/mi11111021DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7700668PMC
November 2020

External beam radiation therapy in a centenarian with primary liver cancer: A case report.

Medicine (Baltimore) 2020 Nov;99(47):e22473

Department of Radiation Oncology, The First Affiliated Hospital of Guangxi Medical University.

Rationale: Due to unprecedented global aging, the number of elderly and super-elderly patients with cancer is increasing. However, restricted by comorbidities or fragility, many elderly patients are considered ineligible to receive invasive therapies. A centenarian with primary liver cancer (PLC) was treated by external beam radiation therapy (EBRT). This rare case deserves our attention.

Patient Concerns: We present a rare case of a centenarian with PLC. The super-elderly male patient complained that 2 liver lesions were found by abdominal ultrasonography in June 2016.

Diagnoses: The Segment 7 (S7) lesion and the Segment 5/8 (S5/8) lesion were clinically diagnosed as PLC successively.

Interventions: The S7 lesion was considered PLC initially and treated by EBRT in October 2016. In the 1-year follow-up after EBRT, the S7 lesion was well controlled. Unfortunately, the S5/8 lesion had increased in size, was diagnosed as PLC and subsequently treated by CyberKnife in another hospital. However, local failure of the S5/8 lesion was suggested 15 months after CyberKnife. At the age of 102 years, the patient received re-irradiation for the S5/8 lesion.

Outcomes: Three months after re-irradiation, des-γ-carboxy-prothrombin decreased to normal; no significant change in the S5/8 lesion was found in Magnetic Resonance Imaging. No severe acute or late toxicities were reported after each course of EBRT. Unfortunately, the patient died of respiratory failure caused by severe pneumonia in mid-March 2020.

Conclusion: Advanced age is not a contraindication for elderly patients with cancer to receive radiotherapy and even re-irradiation.
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http://dx.doi.org/10.1097/MD.0000000000022473DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7676518PMC
November 2020

[Retracted] Epigenetic silencing of miR‑130a ameliorates hemangioma by targeting tissue factor pathway inhibitor 2 through FAK/PI3K/Rac1/mdm2 signaling.

Int J Oncol 2020 12 20;57(6):1383. Epub 2020 Oct 20.

Department of Aesthetic, Plastic and Burn Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shangdong 250021, P.R. China.

Following the publication of this paper, it was drawn to the authors' attention by an interested reader that some tumours featured in Fig. 6A of the above paper were strikingly similar to those featured in Fig. 8A of an article appearing in the same journal [Fan F‑Y, Deng R, Yi H, Sun H‑P, Zeng Y, He G‑C and Su Y: The inhibitory effect of MEG3/miR‑214/AIFM2 axis on the growth of T‑cell lymphoblastic lymphoma. Int J Oncol 51: 316‑326, 2017]. Furthermore, flow cytometric images featured in Fig. 2G of the above paper were strikingly similar to data featured in the following article [Zhang H‑j, Wei Q‑f, Wang S‑j, Zhang H‑j, Zhang X‑y, Geng Q, Cui Y‑h and Wang X‑h: LncRNA HOTAIR alleviates rheumatoid arthritis by targeting miR‑138 and inactivating NF‑κB pathway. Int Immunopharmacol 50: 283‑290, 2017]. The Editor asked the authors for an explanation to account for the appearance of strikingly similar data in their paper independently, and they responded to request that the paper be retracted from International Journal of Oncology. All the authors agreed that the article should be retracted. The Editor apologizes to the readership for any inconvenience caused. [the original article was published in International Journal of Oncology 50: 1821‑1831, 2017; DOI: 10.3892/ijo.2017.3943].
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http://dx.doi.org/10.3892/ijo.2020.5136DOI Listing
December 2020

Structural characterization of enzymatic modification of Hericium erinaceus polysaccharide and its immune-enhancement activity.

Int J Biol Macromol 2021 Jan 6;166:1396-1408. Epub 2020 Nov 6.

Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health in Fujian province, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China. Electronic address:

In this study, the enzyme degradation of Hericium erinaceus polysaccharide (HEP) was successfully modified with endo-rhamnosidase to obtain the enzymatic hydrolysis of Hericium erinaceus polysaccharide product (EHEP). The gas chromatography-mass spectrometry (GC-MS), high performance gel permeation chromatography (HPGPC), Fourier transformed infrared spectrometry (FT-IR), scanning electron microscopy (SEM), atomic particle microscopy (AFM), nuclear magnetic resonance (NMR) and particle size distribution were used to characterize polysaccharides. In vitro, EHEP significantly enhanced the phagocytosis, NO, CD40 and CD86 by macrophage than HEP. In vivo, female Balb/c mice were injected respectively with EHEP and HEP after administrated with cyclophosphamide, once a day for 7 days. On days 11, the morphology and structure of jejunal sections, immunofluorescence of spleen and peritoneal macrophages were determined. These results indicated that the enzymatic hydrolysis product could enhance the activation of peritoneal macrophages, and enhance the immunomodulation function of HEP. This study demonstrated that enzymatic modification was an effective method to improve the activities of HEP, and could be developed as a potential technology for use in pharmaceutical and cosmeceutical industry.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.11.019DOI Listing
January 2021

Traditional Chinese medicine for oral squamous cell carcinoma: A Bayesian network meta-analysis protocol.

Medicine (Baltimore) 2020 Oct;99(43):e22955

Department of Stomatology, Liaocheng People's Hospital.

Background: Traditional Chinese medicine is frequently used for malignant tumors in China, but in clinical practice, most practitioners choose appropriate Chinese medicines based on personal experience. In our study, Bayesian network meta-analysis will be used to identify differences in efficacy and safety between diverse traditional Chinese drugs for oral squamous cell carcinoma (OSCC).

Methods: Relevant randomized controlled trials and prospective controlled clinical trials were searched from Medline, PubMed, Cochrane Library, Google Scholar, Excerpt Medica Database, Web of Science, China National Knowledge Infrastructure, China Scientific Journal Database, Chinese Biomedical Literature Database, and Wanfang Database from their establishment to September 2020. Study selection and data extraction will be performed independently by 2 researchers. Aggregate Data Drug Information System and R software were used for data synthesis. The evidentiary grade of the results will be also evaluated.

Results: The results of this study will be published in a peer-reviewed journal, and provide reliable evidence for different traditional Chinese drugs on OSCC.

Conclusions: The findings will provide reference for evaluating the efficacy and safety of different traditional Chinese medicine for OSCC, and provide a helpful evidence for clinicians to formulate the best adjuvant treatment strategy for OSCC patients.

Trial Registration Number: INPLASY202090082.
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http://dx.doi.org/10.1097/MD.0000000000022955DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7581022PMC
October 2020

gcType: a high-quality type strain genome database for microbial phylogenetic and functional research.

Nucleic Acids Res 2021 01;49(D1):D694-D705

Microbial Resource and Big Data Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.

Taxonomic and functional research of microorganisms has increasingly relied upon genome-based data and methods. As the depository of the Global Catalogue of Microorganisms (GCM) 10K prokaryotic type strain sequencing project, Global Catalogue of Type Strain (gcType) has published 1049 type strain genomes sequenced by the GCM 10K project which are preserved in global culture collections with a valid published status. Additionally, the information provided through gcType includes >12 000 publicly available type strain genome sequences from GenBank incorporated using quality control criteria and standard data annotation pipelines to form a high-quality reference database. This database integrates type strain sequences with their phenotypic information to facilitate phenotypic and genotypic analyses. Multiple formats of cross-genome searches and interactive interfaces have allowed extensive exploration of the database's resources. In this study, we describe web-based data analysis pipelines for genomic analyses and genome-based taxonomy, which could serve as a one-stop platform for the identification of prokaryotic species. The number of type strain genomes that are published will continue to increase as the GCM 10K project increases its collaboration with culture collections worldwide. Data of this project is shared with the International Nucleotide Sequence Database Collaboration. Access to gcType is free at http://gctype.wdcm.org/.
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http://dx.doi.org/10.1093/nar/gkaa957DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7778895PMC
January 2021

Carboxyl graphene oxide nanoparticles induce neurodevelopmental defects and locomotor disorders in zebrafish larvae.

Chemosphere 2021 May 15;270:128611. Epub 2020 Oct 15.

Jiangxi Engineering Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases, Jinggangshan University, Ji'an, 343009, Jiangxi, China; Jiangxi Key Laboratory of Developmental Biology of Organs, Jinggangshan University, Ji'an, 343009, Jiangxi, China; Affiliated Hospital of Jinggangshan University, College of Life Sciences, Jinggangshan University, Ji'an, 343009, Jiangxi, China; Jiangxi Engineering Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases, Ji'an, Jiangxi, China; Jiangxi Key Laboratory of Developmental Biology of Organs, Ji'an, 343009 Jiangxi, China. Electronic address:

Graphene-family nanomaterials (GFNs) have been widely used in various fields due to their excellent properties. However, GFNs safety and environmental health have attracted more and more attentions and their potential toxic effects on organisms and the underlying mechanisms are still poorly understood. In this study, we utilized zebrafish to evaluate the toxicity of Carboxyl graphene oxide (GO-COOH). Exposure of zebrafish embryos to 10, 50 and 100 mg/L GO-COOH specifically induced neurodevelopmental abnormalities and altered tendency of locomotor in larval fish. Furthermore, GO-COOH exposure led to increase of AchE and ATPase activities and oxidative stress upregulation, and disrupted the expression of genes involved in neurodevelopment and neurotransmitter pathway. Interestingly, we found that Parkinson's disease-related genes' expression were disordered after GO-COOH treatment. Fullerenes and astaxanthin rescued the neurodevelopmental defects, tendency of locomotor and expression of Parkinson's disease-related genes caused by GO-COOH through downregulating oxidative stress. Therefore, our results suggest that GO-COOH has the potential to induce neurotoxicity and Parkinson's disease-like symptoms in zebrafish larvae.
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http://dx.doi.org/10.1016/j.chemosphere.2020.128611DOI Listing
May 2021

Optically transparent coding metasurface with simultaneously low infrared emissivity and microwave scattering reduction.

Opt Express 2020 Sep;28(19):27774-27784

In this paper, an optically transparent coding metasurface structure based on indium tin oxide (ITO) thin films with simultaneously low infrared (IR) emissivity and microwave scattering reduction is proposed. To this end, two ITO coding elements which can reflect 0° and 180° phase responses are firstly designed. Based on these two elements, four coding sequences with different scattering patterns are designed. Three of them can realize anomalous reflections and the fourth can realize random diffusion of normal incident electromagnetic (EM) waves. A prototype of the random diffusion coding metasurface was fabricated and measured. The experimental results show that for normal incident EM waves, at least 10dB backward scattering reduction from 3.8GHz to 6.8GHz can be achieved, and the structure is polarization insensitive. The averaged transmittance of visible light through the coding metasurface reaches up to 72.2%. In addition, due to the high occupation ratio of ITO on the outside of the coding metasurface, a low IR emissivity of about 0.275 is obtained. Good consistency between the experiment and simulation results convincingly verifies the coding metasurface. Due to its multispectral compatibility, the proposed coding metasurface may find potential applications in multi-spectral stealth, camouflage, etc.
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http://dx.doi.org/10.1364/OE.402326DOI Listing
September 2020

Protocol for a systematic review and meta-analysis of Kang-ai injection for patients with oesophageal cancer.

Medicine (Baltimore) 2020 Sep;99(36):e22148

Department of Gastroenterology, Liaocheng People's Hospital, Liaocheng, Shandong Province, P.R. China.

Background: Oesophageal cancer (OC) is the sixth leading cause of cancer death worldwide. Despite the improvement of therapeutic methods in recent years, the prognosis of OC remains unsatisfactory. Kang-ai injection, a kind of traditional Chinese herbal medicine, has been widely applied as a promising adjunctive drug for OC. In this study, we aimed to summarize the efficacy and safety of Kang-ai injection for patients with advanced OC through the meta-analysis, in order to provide scientific reference for the design of future clinical trials.

Methods: Relevant randomized controlled trials and high-quality prospective cohort studies were searched from PubMed, Web of Science, Medline, Cochrane Library, Google Scholar, Excerpt Medica Database, Chinese Biomedical Literature Database, China National Knowledge Infrastructure, China Scientific Journal Database and Wanfang Database. Papers in English or Chinese published from their inception to August 2020 will be included without any restrictions.Study selection and data extraction will be performed independently by 2 investigators. The clinical outcomes including overall response rate, disease control rate, overall survival, disease-free survival, quality of life, immune function and adverse events, were systematically evaluated. Stata 14.0 and Review Manager 5.3 were used for data synthesis, subgroup analysis, sensitivity analysis, meta regression, and risk of bias assessment.

Results: The results of this study will be published in a peer-reviewed journal, or presented the findings at a relevant conference.

Conclusion: Our study will draw an objective conclusion of the effects of Kang-ai injection combined with conventional treatment for advanced OC and provide a helpful evidence for clinicians to formulate the best postoperative adjuvant treatment strategy for OC patients.

Inplasy Registration Number: INPLASY202080019.
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http://dx.doi.org/10.1097/MD.0000000000022148DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7478825PMC
September 2020

Analytical method development for α-amanitin and β-amanitin in plasma at ultra-trace level by online solid phase extraction-high performance liquid chromatography-triple quadrupole mass spectrometry and its application in poisoning events.

J Pharm Biomed Anal 2020 Oct 8;190:113523. Epub 2020 Aug 8.

Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, 310051, China. Electronic address:

α-Amanitin and β-amanitin are the main fatal mushroom toxins. The toxins metabolize rapidly in blood and are reported hard to be detected 24 h after poisoning. The main challenge is that of developing a highly sensitive method at sub-pg mL level in blood to diagnose intoxication cause and to study the poisoning mechanism and blood toxicity kinetics. An analytical method for α-amanitin and β-amanitin at ultra-trace level was developed in this study by online solid phase extraction-high performance liquid chromatography-triple quadrupole mass spectrometry (online SPE-LC-MS/MS). Simple protein precipitation and liquid-liquid extraction were introduced to resolve the sample preparation problem of the online SPE-LC-MS/MS system with large-volume injection. A quick valve-switching technique with a quantitative loop as interface was used in the online system. This design can ensure the independence of flow path and pressure between the SPE and LC-MS/MS modules and can obtain the precise cleanup of the toxins. The limits of detection for α-amanitin and β-amanitin in plasma were both 0.02 ng mL. The linear ranges were 0.05-20 ng mL with a correlation coefficient r >0.99. The average recoveries at three spiking levels were 82.9 %-92.2 % with the relative standard deviations (RSD) of 5.4 %-8.0 % for α-amanitin and 84.5 %-93.9 % with RSDs of 4.5 %-7.8 % for β-amanitin. The composition and concentration of the toxins in plasma from 18 patients in 5 mushroom poisoning events caused by aminitins were studied. The developed method has high positive confirmation ability and can identify toxins in plasma 40 h after poisoning.
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http://dx.doi.org/10.1016/j.jpba.2020.113523DOI Listing
October 2020

[Clinical Efficacy and Prognosis of Double-Hit Multiple Myeloma Patients with Deletion P53 Treated with Regimen Based on Bortezomib].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2020 Aug;28(4):1221-1227

Department of Hematology, Harrison International Peace Hospital, Hengshui 053000, Hebei Province,

Objective: To investigate the clinical efficacy and prognosis of double-hit multiple myeloma patients with deletion P53 treated with regimen based on bortezomib.

Methods: The ethnical data from 186 newly diagnosed MM patients hospitalized in the Department of Hematology of Harrison International Peace hospital from January 2012 to January 2019 were analyzed retrospectively. The fluorescent in situ hybridization (FISH) and G-binding staining were used to detect cytogenetic abnormalities (P53 deletion, lq21 amplification and IgH rearranagement) for analyses of complete remission (CR), overall response rate (ORR), progression-free survival (PFS) and overall survival (OS) of patients treated with bortezomib for 4 circles.

Results: In 186 patients, simple P53 deletion was 14 cases, 1q21 amplification and P53 deletion were found in 11 cases (A group), t (14;16) and P53 deletion in 7 cases (B group), t (4;14) and P53 deletion in 9 cases (C group). The complete remission rate (CR%) of above-mentioned three groups was 27.27%, 28.57% and 33.33% respectively, and the ORR of the three groups was 54.54%, 57.14% and 55.56%, respectively, there was no statistically significant difference between the three groups (P>0.05). The patients with 1q21 amplification and P53 deletion had shorter OS and PFS time (P=0.041, P=0.046). The double-hit patients with 1q21 amplification showed shorter OS time, compared with the patients with P53 deletion (P=0.027). The double-hit patients with t(14;16) and t(4;14) showed shorter OS time (P=0.871, P=0.276) and PFS time (P=0.955, P=0.379) than those of the patients with P53 deletion.

Conclusion: P53 deletion and 1q21 amplification are an adverse prognostic factor of early recurrence and short lifetime in patients with newly diagnosed double-hit MM.
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http://dx.doi.org/10.19746/j.cnki.issn.1009-2137.2020.04.023DOI Listing
August 2020

Knockdown of Tcirg1 inhibits large-osteoclast generation by down-regulating NFATc1 and IP3R2 expression.

PLoS One 2020 13;15(8):e0237354. Epub 2020 Aug 13.

Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Shandong University & Shandong Provincial Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Jinan, Shandong, PR China.

The TCIRG1 gene encodes the a3 isoform of vacuolar H+-ATPase (V-ATPase), which forms a proton transport channel in osteoclasts. Defects in this gene lead to functional impairment of osteoclasts and increased bone mass; however, the molecular mechanisms of TCIRG1 loss have not been fully elucidated. In the current study, we transfected mouse bone marrow-derived monocytes with control or Tcirg1-knockdown lentiviruses to further investigate the mechanisms of TCIRG1. Our results demonstrate that knockdown of Tcirg1 inhibits large-osteoclast (>100 μm) generation by decreasing the expression of nuclear factor of activated T-cells 1 (NFATc1) and inositol-1,4,5-trisphosphate receptor 2 (IP3R2). The decreased IP3R2 reduces intracellular calcium levels, which limits the nuclear translocation of NFATc1 in RANKL-induced mouse bone marrow-derived monocytes. These findings provide a mechanism to explain the effects of TCIRG1 impairment, with potential implications for the development of therapies for osteopetrosis.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0237354PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7425954PMC
October 2020

Truncated tests for combining evidence of summary statistics.

Genet Epidemiol 2020 10 24;44(7):687-701. Epub 2020 Jun 24.

School of Mathematical Sciences, University of Chinese Academy of Sciences, Beijing, China.

To date, thousands of genetic variants to be associated with numerous human traits and diseases have been identified by genome-wide association studies (GWASs). The GWASs focus on testing the association between single trait and genetic variants. However, the analysis of multiple traits and single nucleotide polymorphisms (SNPs) might reflect physiological process of complex diseases and the corresponding study is called pleiotropy association analysis. Modern day GWASs report only summary statistics instead of individual-level phenotype and genotype data to avoid logistical and privacy issues. Existing methods for combining multiple phenotypes GWAS summary statistics mainly focus on low-dimensional phenotypes while lose power in high-dimensional cases. To overcome this defect, we propose two kinds of truncated tests to combine multiple phenotypes summary statistics. Extensive simulations show that the proposed methods are robust and powerful when the dimension of the phenotypes is high and only part of the phenotypes are associated with the SNPs. We apply the proposed methods to blood cytokines data collected from Finnish population. Results show that the proposed tests can identify additional genetic markers that are missed by single trait analysis.
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http://dx.doi.org/10.1002/gepi.22330DOI Listing
October 2020

[Clinical Efficacy of Low-Dose Bortezomib-Based Triple Combination Therapy in the Treatment of Elderly Multiple Myeloma].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2020 Apr;28(2):535-539

Department of Hematology, Harrison International Peace Hospital, Hengshui 053000, Hebei Province, China,E-mail:

Objective: To investigate the clinical efficacy and safety of low-dose bortezomib-based triple combination therapy in the treatment of elderly multiple myeloma(MM).

Methods: Fifty-eight patients with elderly multiple myeloma were selected. And according to the drug rigimen the patients were randomly divided into three groups. Group A: patients received low-dose bortezomib(0.7mg/m) on day 1, 4, 8 and 11; cyclophosphamide (0.5 g/m) d 1, d 8; dexamethasone d 1-2,d 4-5, d 8-11, d 11-12. Group B: patients received bortezomib (1.3 mg/m) on day 1, 4, 8 and 11; cyclophosphamide (0.5 g/m) d 1, d 8; dexamethasone d 1-2, d 4-5, d 8-11, d 11-12. Group C: patients received bortezomib(0.7 mg/m) on day 1, 4, 8 and 11; dexamethasone d 1-2, d 4-5, d 8-11, d 11-12. All patients in three groups were treated for 4 circles. The efficacy and response of the three groups were compared.

Results: The complete remission (CR) rate in the three groups was 31.58%, 38.09% and 27.78%. And the overall response (ORR) rate of the three groups was 68.42%, 66.67% and 55.56%, there was no statistical difference among the three groups (P>0.05). The Hb level in MM patients with anemia after treatment was higher than the basic value. There were no statistical differences in percapita red blood cell transfusion, the incidence of bone marrow suppression and infection during 4 courses treatment among 3 groups, but the incidence of peripheral neuropathy, gastrointestinal reaction and herper zoster in group A was significantly lower than that in group B and C, moreover there was statistical difference. After the follow-up for 1 year, there was no significant difference in the survival rate among 3 groups.

Conclusion: The efficacy of low dose bortezomib-based triple combination therapy for the patients with elderly multiple mycloma is same efficacy as of standard dose bortezomib-based triple combination therapy, but the incidence of perpheral neuropathy, gastrointestinal reaction and herper zoster is lower, the patients have better torelonce to low dose bortezomib-based triple combination therapy regimen, suggesting that this therapy possesses the efficacy of less adverse reactions and more high safety in treatment of patients with elderly muitipe myelome.
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http://dx.doi.org/10.19746/j.cnki.issn.1009-2137.2020.02.030DOI Listing
April 2020

Preoperative neoadjuvant chemotherapy combined with radical surgery in cervical cancer.

J BUON 2020 Jan-Feb;25(1):125-131

Department of Clinical Laboratory, The People's Hospital of Zhangqiu Area, Jinan 250200, P.R.China.

Purpose: To investigate the efficacy of preoperative neoadjuvant chemotherapy combined with radical surgery on cervical cancer patients and its effect on the prognosis of patients and serum squamous cell carcinoma antigen (SCC-Ag) level.

Methods: 163 patients with cervical cancer composed the research group. Among them, 72 patients treated with radical resection of cervical cancer were enrolled in the control group, while the remaining 91 patients were enrolled in the study group and treated with preoperative neoadjuvant chemotherapy combined with radical resection of cervical cancer. The clinical indicators during and after the surgery, as well as the efficacy, were compared between the two groups. Serum SCC-Ag level before and after the treatment was measured by enzyme-linked immunosorbent assay (ELISA).

Results: The total effective rate of the study group was statistically higher than that of the control group (p<0.05). Seven cases (9.72%) of adverse reactions occurred in the control group. Thirteen cases (12.09%) of adverse reactions occurred in the study group. The serum SCC-Ag levels of the two groups after the treatment were significantly lower than those before the treatment, with a sharper decrease in the study group (p<0.05).

Conclusion: Preoperative neoadjuvant chemotherapy combined with radical surgery has remarkable efficacy in cervical cancer patients, and can significantly reduce serum SCC-Ag levels in patients, which is worthy of clinical promotion.
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December 2020

Identification of a novel fusion gene, TRIM52-RACK1, in oral squamous cell carcinoma.

Mol Cell Probes 2020 08 3;52:101568. Epub 2020 Apr 3.

Key Lab of Precision Biomedicine & Department of Stomatology, Liaocheng People's Hospital, College of Stomatology, Shandong First Medical University, China; Medical College, Liaocheng University, China. Electronic address:

Gene fusion is caused by the linkage of previously separate genes or sequences. Recently, an increasing number of novel fusion genes have been identified and associated with tumor progression, and several of them have been suggested as promising targets for tumor therapy. However, there are hardly any studies reporting the association of fusion genes with the progression of oral squamous cell carcinoma (OSCC). In this study, we identified a total of 11 fused genes in OSCC cells. We further analyzed the structure of one fused gene, TRIM52-RACK1, and detected its function in tumor progression in vitro. We found that TRIM52-RACK1 was caused by a deletion of 181,257,187-181,247,386 at 5q35.3 and it promoted OSCC cell proliferation, migration, and invasion. Therefore, TRIM52-RACK1 can be a promising target for tumor therapy in OSCC.
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http://dx.doi.org/10.1016/j.mcp.2020.101568DOI Listing
August 2020

LncRNA PVT1 induces chondrocyte apoptosis through upregulation of TNF-α in synoviocytes by sponging miR-211-3p.

Mol Cell Probes 2020 08 21;52:101560. Epub 2020 Mar 21.

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST), Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, 237 Luoyu Rd, Wuhan, 430079, China; Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Wuhan University, Wuhan, 430079, China. Electronic address:

Temporomandibular joint osteoarthritis (TMJ OA) is an important subtype of temporomandibular disorders (TMD). Articular cartilage destruction is considered a common pathological feature of TMJ OA, which is reported to be mainly induced by chondrocyte apoptosis. Synovial sterile inflammation is an initial factor of TMJ OA-associated articular cartilage destruction. Therefore, determining the mechanism of synovial membrane inflammation-induced articular cartilage destruction in TMJ OA is important for the TMJ OA therapy. In this study, we detected the function of synoviocytes in chondrocyte apoptosis under lipopolysaccharide (LPS)-induced inflammatory conditions and explored the underlying mechanism. We found that synoviocytes in inflammatory conditions facilitated LPS-induced chondrocytes apoptosis by secreting increased Tumor Necrosis Factor α (TNF-α), which was induced by long non-coding RNA plasmacytoma variant translocation 1 (PVT1) upregulation. PVT1 served as a competing endogenous RNA that sponged the microRNA miR-211-3p and prevented the inhibition of TNF-α expression. In conclusion, our in vitro study revealed that PVT1 has a previously unknown role in chondrocyte apoptosis, which may also be a mechanism underlying synoviocyte involvement in TMJ OA.
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http://dx.doi.org/10.1016/j.mcp.2020.101560DOI Listing
August 2020

o-Phenylenediamine/gold nanocluster-based nanoplatform for ratiometric fluorescence detection of alkaline phosphatase activity.

Talanta 2020 May 21;212:120768. Epub 2020 Jan 21.

College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu Shandong, 273165, PR China. Electronic address:

This study demonstrates a novel and convenient ratiometric fluorescent method for the detection of alkaline phosphatase (ALP) activity. Amino-functionalized mesoporous silica nanoparticle-gold nanoclusters (MSN-AuNCs) nanocomposites were integrated with o-phenylenediamine (OPD) to form a ratiometric fluorescence nanoplatform. The presence of ALP induced the generation of quinoxaline (QX) derivative which called 3-(dihydroxyethyl)furo[3,4-b]quinoxaline-1-one (DFQ) with strong fluorescence emission at 450 nm, while the orange-red fluorescence of MSN-AuNCs at 580 nm was slightly quenched. Meanwhile, an obvious fluorescence color change from orange-red to purple and finally to blue can be observed by naked eyes with the increasing of ALP concentration. Therefore, employing the fluorescence emission of DFQ at 450 nm as the reporter signal and the fluorescence emission of MSN-AuNCs at 580 nm as a reference signal, a sensitive ratiometric detection method for ALP was developed. Quantitative detection of ALP activity in the linear range from 0.2 to 80 U/L with a detection limit of 0.1 U/L can be realized in this way, which endows the assay with high sensitivity enough for practical detection of ALP in human serum samples.
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http://dx.doi.org/10.1016/j.talanta.2020.120768DOI Listing
May 2020
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