Publications by authors named "Zhen Ma"

302 Publications

Design, Synthesis, and Bioactivity of Novel Bifunctional Small Molecules for Alzheimer's disease.

ACS Omega 2022 Aug 20;7(30):26308-26315. Epub 2022 Jul 20.

Affiliated Yongkang First People's Hospital and School of Pharmacy, Hangzhou Medical College, Hangzhou, Zhejiang 310013, China.

The abnormal phosphorylation of the τ-protein is a typical early pathological feature of Alzheimer's disease (AD). The excessive phosphorylation of the τ-protein in the brain causes the formation of neurofibrillary tangles (NFTs) and increases the neurotoxicity of amyloid-β (Aβ). Thus, targeting the τ-protein is considered a promising strategy for treating AD. Herein, we designed and synthesized a series of molecules containing bifunctional groups to recognize the τ-protein and the E3 ligase. The molecules were examined , and their effects were tested on PC12 cells. In addition, we further studied the pharmacokinetics of compound I3 in healthy rats. Our data showed that compound I3 could effectively degrade τ-protein, reduce Aβ-induced cytotoxicity, and regulate the uneven distribution of mitochondria, which may open a new therapeutic strategy for the treatment of AD.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsomega.2c02130DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9352321PMC
August 2022

Application of intraoperative photodynamic therapy in patients suspected of recurrence post radical surgery: A single center experience.

Photodiagnosis Photodyn Ther 2022 Aug 2:103047. Epub 2022 Aug 2.

The Second Clinical Medical College, Lanzhou University, Lanzhou 730030, China; The Department of Tumor Surgery, Lanzhou University Second Hospital, Lanzhou 730030, China. Electronic address:

Background: Difficult tumors can be treated with a combination of radical surgery and photodynamic therapy to reduce recurrences. The aim of this study is to present a combined application of radical surgery combined with intraoperative PDT approach in patients with various cancers suspected of post-operative recurrence from a single center.

Methods: Radical surgery combined with intraoperative PDT was performed in each and every patient under study at different time points from June 2020 to July 2021, and the PDT irradiation time ranged from 10, 20, 25 and 30 minutes. Hematoporphyrin, as a photo synthesizer, was administered intravenously 48 hours before surgery and during the operative period respectively, at a 3mg/kg dose. In addition, the mean and median survival times for each of these patients were also evaluated. Patient's overall disease-Free Survival (DFS) and survival (OS) were immensely evaluated.

Results: 12 patients (33.3% female and 66.7 % male) underwent radical surgery and PDT simultaneously. No photosensitivity events were reported in the included patients, except for one case with a moderate to severe erythema. Intraoperative PDT was tolerated in all included patients without serious liver and kidney damages. As from the time these patients underwent radical surgery and PDT, three mortalities were recorded and the remaining 9 patients had some remarkable outcomes with less or no recurrences.

Conclusions: Intraoperative PDT is a safe therapeutic strategy for various tumor patients who undergo operation. Intraoperative PDT combined with surgery may be a novel therapeutic option that need enrollment of more tumor patients to evaluate its efficacy in the future.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.pdpdt.2022.103047DOI Listing
August 2022

Advances in dietary proteins binding with co-existed anthocyanins in foods: Driving forces, structure-affinity relationship, and functional and nutritional properties.

Crit Rev Food Sci Nutr 2022 Jun 24:1-22. Epub 2022 Jun 24.

Shanghai Food Safety and Engineering Technology Research Center, Bor S. Luh Food Safety Research Center, Key Lab of Urban Agriculture (South), School of Agriculture & Biology, Shanghai Jiao Tong University, Shanghai, China.

Anthocyanins, which are the labile flavonoid pigments widely distributed in many fruits, vegetables, cereal grains, and flowers, are receiving intensive interest for their potential health benefits. Proteins are important food components from abundant sources and present high binding affinity for small dietary compounds, e.g., anthocyanins. Protein-anthocyanin interactions might occur during food processing, ingestion, digestion, and bioutilization, leading to significant changes in the structure and properties of proteins and anthocyanins. Current knowledge of protein-anthocyanin interactions and their contributions to functions and bioactivities of anthocyanin-containing foods were reviewed. Binding characterization of dietary protein-anthocyanins complexes is outlined. Advances in understanding the structure-affinity relationship of dietary protein-anthocyanin interaction are critically discussed. The associated properties of protein-anthocyanin complexes are considered in an evaluation of functional and nutritional values.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/10408398.2022.2086211DOI Listing
June 2022

Complement C3a activates astrocytes to promote medulloblastoma progression through TNF-α.

J Neuroinflammation 2022 Jun 20;19(1):159. Epub 2022 Jun 20.

Laboratory of Molecular Neuropathology, Pediatric Cancer Center, College of Pharmaceutical Sciences, Soochow University, Suzhou, China.

Background: Medulloblastoma (MB) is the most common malignant brain tumor in children. Approximately one-third of MB patients remain incurable. Understanding the molecular mechanism of MB tumorigenesis is, therefore, critical for developing specific and effective treatment strategies. Our previous work demonstrated that astrocytes constitute the tumor microenvironment (TME) of MB and play an indispensable role in MB progression. However, the underlying mechanisms by which astrocytes are regulated and activated to promote MB remain elusive.

Methods: By taking advantage of Math1-Cre/Ptch1 mice, which spontaneously develop MB, primary MB cells and astrocytes were isolated and then subjected to administration and coculture in vitro. Immunohistochemistry was utilized to determine the presence of C3a in MB sections. MB cell proliferation was evaluated by immunofluorescent staining. GFAP and cytokine expression levels in C3a-stimulated astrocytes were assessed by immunofluorescent staining, western blotting, q-PCR and ELISA. C3a receptor and TNF-α receptor expression was determined by PCR and immunofluorescent staining. p38 MAPK pathway activation was detected by western blotting. Transplanted MB mice were treated with a C3a receptor antagonist or TNF-α receptor antagonist to investigate their role in MB progression in vivo.

Results: We found that complement C3a, a fragment released from intact complement C3 following complement activation, was enriched in both human and murine MB tumor tissue, and its receptor was highly expressed on tumor-associated astrocytes (TAAs). We demonstrated that C3a activated astrocytes and promoted MB cell proliferation via the p38 MAPK pathway. Moreover, we discovered that C3a upregulated the production of proinflammatory cytokines, such as IL-6 and TNF-α in astrocytes. Application of the conditioned medium of C3a-stimulated astrocytes promoted MB cell proliferation, which was abolished by preincubation with a TNF-α receptor antagonist, indicating a TNF-α-dependent event. Indeed, we further demonstrated that administration of a selective C3a receptor or TNF-α receptor antagonist to mice subcutaneously transplanted with MB suppressed tumor progression in vivo.

Conclusions: C3a was released during MB development. C3a triggered astrocyte activation and TNF-α production via the p38 pathway, which promoted MB cell proliferation. Our findings revealed the novel role of C3a-mediated TNF-α production by astrocytes in MB progression. These findings imply that targeting C3a and TNF-α may represent a potential novel therapeutic approach for human MB.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12974-022-02516-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9208237PMC
June 2022

High stability of autochthonous dissolved organic matter in karst aquatic ecosystems: Evidence from fluorescence.

Water Res 2022 Jul 7;220:118723. Epub 2022 Jun 7.

State Key Laboratory of Environmental Geochemistry, CAS, Institute of Geochemistry, Guiyang 550081, China.

Biological carbon pump (BCP) in karst areas has received intensive attention for years due to their significant contribution to the global missing carbon sink. The stability of autochthonous dissolved organic matter (Auto-DOM) produced by BCP in karst aquatic ecosystems may play a critical role in the missing carbon sink. However, the source of dissolved organic matter (DOM) in inland waters and its consumption by planktonic bacteria have not been thoroughly examined. Recalcitrant dissolved organic matter (RDOM) may exist in karst aquatic ecosystem as in the ocean. Through the study of the chromophoric dissolved organic matter (CDOM) and the interaction between CDOM and the planktonic bacterial community under different land uses at the Shawan Karst Water-carbon Cycle Test Site, SW China, we found that C2, as the fluorescence component of Auto-DOM mineralised by planktonic bacteria, may have some of the characteristics of RDOM and is an important DOM source in karst aquatic ecosystems. The stability ratio (Fmax) of Auto-DOM reached 89.6 ± 6.71% in winter and 64.1 ± 7.19% in spring. Moreover, correlation-based network analysis determined that the planktonic bacterial communities were controlled by different fluorescence types of CDOM, of which C1 (fresh Auto-DOM), C3 (conventional allochthonous DOM (Allo-DOM)) and C4 (the Allo-DOM mineralised by bacteria) were clustered in one module together with prevalent organic-degrading planktonic bacteria; C2 was clustered in another tightly combined module, suggesting specific microbial utilization strategies for the C2 component. In addition, some important planktonic bacterium and functional genes (including chemotrophic heterotrophs and photosynthetic bacteria) were found to be affected by high Ca and dissolved inorganic carbon (DIC) concentrations in karst aquatic ecosystems. Our research showed that Auto-DOM may be as an important carbon sink as the Allo-DOM in karst ecosystems, the former generally being neglected based on a posit that it is easily and first mineralized by planktonic bacteria.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.watres.2022.118723DOI Listing
July 2022

Integrating nonlinear analysis and machine learning for human induced pluripotent stem cell-based drug cardiotoxicity testing.

J Tissue Eng Regen Med 2022 Aug 27;16(8):732-743. Epub 2022 May 27.

Department of Biomedical & Chemical Engineering, Syracuse University, Syracuse, New York, USA.

Utilizing recent advances in human induced pluripotent stem cell (hiPSC) technology, nonlinear analysis and machine learning we can create novel tools to evaluate drug-induced cardiotoxicity on human cardiomyocytes. With cardiovascular disease remaining the leading cause of death globally it has become imperative to create effective and modern tools to test the efficacy and toxicity of drugs to combat heart disease. The calcium transient signals recorded from hiPSC-derived cardiomyocytes (hiPSC-CMs) are highly complex and dynamic with great degrees of response characteristics to various drug treatments. However, traditional linear methods often fail to capture the subtle variation in these signals generated by hiPSC-CMs. In this work, we integrated nonlinear analysis, dimensionality reduction techniques and machine learning algorithms for better classifying the contractile signals from hiPSC-CMs in response to different drug exposure. By utilizing extracted parameters from a commercially available high-throughput testing platform, we were able to distinguish the groups with drug treatment from baseline controls, determine the drug exposure relative to IC50 values, and classify the drugs by its unique cardiac responses. By incorporating nonlinear parameters computed by phase space reconstruction, we were able to improve our machine learning algorithm's ability to predict cardiotoxic levels and drug classifications. We also visualized the effects of drug treatment and dosages with dimensionality reduction techniques, t-distributed stochastic neighbor embedding (t-SNE). We have shown that integration of nonlinear analysis and artificial intelligence has proven to be a powerful tool for analyzing cardiotoxicity and classifying toxic compounds through their mechanistic action.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/term.3325DOI Listing
August 2022

N-acetylcysteine improves diabetic associated erectile dysfunction in streptozotocin-induced diabetic mice by inhibiting oxidative stress.

J Cell Mol Med 2022 06 20;26(12):3527-3537. Epub 2022 May 20.

Department of Urology, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China.

Oxidative stress appears to play a role in the pathogenesis of diabetes mellitus erectile dysfunction (DMED). This study aimed to investigate the effect of N-acetylcysteine (NAC) on DMED in streptozotocin-induced diabetic mice and to explore potential mechanisms. In the present study, we show that an erectile dysfunction is present in the streptozotocin-induced mouse model of diabetes as indicated by decreases in intracavernous pressure responses to electro-stimulation as well as from results of the apomorphine test of erectile function. After treatment of NAC, the intracavernous pressure was increased. In these DMED mice, oxidative stress and inflammatory responses were significantly reduced within the cavernous microenvironment, while activity of antioxidant enzymes in this cavernous tissue was enhanced after NAC treatment. These changes protected mitochondrial stress damage and a significant decreased in apoptosis within the cavernous tissue of DMED mice. This appears to involve activation of the nuclear factor erythroid 2-like-2 (Nrf2) signalling pathway, as well as suppression of the mitogen-activated protein kinase (MAPK) p38/ NF-κB pathway within cavernous tissue. In conclusion, NAC can improve erectile function through inhibiting oxidative stress via activating Nrf2 pathways and reducing apoptosis in streptozotocin-induced diabetic mice. NAC might provide a promising therapeutic strategy for individuals with DMED.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/jcmm.17394DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9189351PMC
June 2022

Identification of Enhancer RNA CDK6-AS1 as a Potential Novel Prognostic Biomarker in Gastric Cancer.

Front Genet 2022 29;13:854211. Epub 2022 Apr 29.

Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.

This study aimed to confirm the role of enhancer RNAs (eRNAs) in gastric cancer and their clinical utility. We used Cox survival and relevance analysis to identify the candidate eRNAs in gastric cancer and performed Gene Ontology and Reactome pathway enrichment to determine the potential functions of eRNAs. Correlation between eRNA, tumor-infiltrating immune cells, and drug sensitivity was then analyzed. , a long non-coding RNA cyclin-dependent kinase 6, may serve as a poor potential prognostic biomarker candidate in gastric cancer with a positive correlation with its target gene . The low expression group showed more frequent mutated driver genes than the high expression group. Moreover, is involved in a key oncogenic pathway of the cell cycle and RNA transcription. also shows dysregulations and associations with prognosis at the pan-cancer level. This eRNA may also be associated with immune cell infiltration and drug sensitivity. may be a potential prognostic biomarker and chemotherapeutic drug sensitivity predictor in gastric cancer.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fgene.2022.854211DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100412PMC
April 2022

Sex Differences in Mate Choice Preference Characteristics of .

Animals (Basel) 2022 May 7;12(9). Epub 2022 May 7.

Key Laboratory of Environment Controlled Aquaculture, Ministry of Education, Dalian 116023, China.

The mating roles of males and females, to a certain extent, are dynamic and variable. Several factors influence the mate choice process. Nonetheless, the main preference features have not yet been fully understood in . In this study, because of its natural pairing characteristics, was selected to explore the mate choice preferences of different sexes. Specifically, male and female behavioral performances were described and quantified through a "no-choice paradigm" during mate choice. A total of 12 behavioral performances were defined in male mate choice (experiment 1), whereas 14 behavioral performances were defined in female mate choice (experiment 2). According to the obtained results, unselected females did not display any proactive behaviors in experiment 1, whereas unselected males exhibited proactive behaviors in experiment 2, including quivering, nipping, tail beating, swimming up and down, and aggression. It was also found that both male and female individuals tend to express dislike rather than like. Those behaviors with higher frequencies (e.g., quivering) often mean less energy expenditure, thus easier repeatability. Moreover, principal component analysis (PCA) was employed to extract and identify mate choice preference features. Preliminary results indicated that male preferences for a mate were mainly associated with body size, behavioral intention, and appearance, whereas the intensity of female preferences was in the order of body size, appearance, and behavioral intention. In addition, sex hormone levels were associated with mate choices.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ani12091205DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9101118PMC
May 2022

Inhibitors of the ubiquitin proteasome system block myofibril assembly in cardiomyocytes derived from chick embryos and human pluripotent stem cells.

Cytoskeleton (Hoboken) 2021 10 24;78(10-12):461-491. Epub 2022 May 24.

Department of Cell and Developmental Biology, SUNY Upstate Medical University, Syracuse, New York, USA.

Details of sarcomeric protein assembly during de novo myofibril formation closely resemble myofibrillogenesis in skeletal and cardiac myocytes in birds, rodents, and zebrafish. The arrangement of proteins during myofibrillogenesis follows a three-step process: beginning with premyofibrils, followed by nascent myofibrils, and concluding with mature myofibrils (reviewed in Sanger et al., 2017). Assembly and maintenance of myofibrils in living muscle cells. In: Handbook of experimental pharmacology, 2017 [pp. 39-75]. Our aim is to determine if the same pathway is followed in human cardiomyocytes derived from human inducible pluripotent stem cells. We found that the human cardiomyocytes developed patterns of protein organization identical to the three-step series seen in the model organisms cited above. Further experiments showed that myofibril assembly can be blocked at the nascent myofibril by five different inhibitors of the Ubiquitin Proteasome System (UPS) stage in both avian and human cardiomyocytes. With the exception of Carfilzomib, removal of the UPS inhibitors allows nascent myofibrils to proceed to mature myofibrils. Some proteasomal inhibitors, such as Bortezomib and Carfilzomib, used to treat multiple myeloma patients, have off-target effects of damage to hearts in three to 6 % of these patients. These cardiovascular adverse events may result from prevention of mature myofibril formation in the cardiomyocytes. In summary, our results support a common three-step model for the formation of myofibrils ranging from avian to human cardiomyocytes. The Ubiquitin Proteasome System is required for progression from nascent myofibrils to mature myofibrils. Our experiments suggest a possible explanation for the cardiac and skeletal muscle off-target effects reported in multiple myeloma patients treated with proteasome inhibitors.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/cm.21697DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9329232PMC
October 2021

Abatement of Nitrogen Oxides via Selective Catalytic Reduction over Ce-W Atom-Pair Sites.

Environ Sci Technol 2022 05 2;56(10):6631-6638. Epub 2022 May 2.

Department of Environmental Science & Engineering, Fudan University, 2005 Songhu Road, Shanghai 200438, China.

Environmentally benign CeO-WO/TiO catalysts are promising alternatives to commercial toxic VO-WO/TiO for controlling NO emission via selective catalytic reduction (SCR), but the insufficient catalytic activity of CeO-WO/TiO catalysts is one of the obstacles in their applications because of a lack of an in-depth understanding of the CeO-WO interactions. Herein, we design a Ce-W/TiO model catalyst by anchoring Ce-W atom pairs on anatase TiO(001) to investigate the synergy between Ce and W in SCR. A series of characterizations combined with density functional theory calculations and diffuse-reflectance infrared Fourier-transform experiments reveal that there exists a strong electronic interaction within Ce-W atom pairs, leading to a much better SCR performance of Ce-W/TiO compared with that of Ce/TiO and W/TiO. The Ce-W synergy not only shifts down the lowest unoccupied states of Ce near the Fermi level, thus enhancing the abilities in adsorbing and oxidizing NH but also makes the frontier orbital electrons of W delocalized, thus accelerating the activation of O. The deep insight of the Ce-W synergy may assist in the design and development of efficient catalysts with an SCR activity as high as or even higher than VO-WO/TiO.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.est.2c00482DOI Listing
May 2022

Snapback-Free Reverse-Conducting SOI LIGBT with an Integrated Self-Biased MOSFET.

Nanoscale Res Lett 2022 Apr 18;17(1):46. Epub 2022 Apr 18.

The State Key Laboratory of Electronic Thin Films and Integrated Devices, University of Electronic Science and Technology of China, Chengdu, 610054, China.

A novel snapback-free RC-LIGBT with integrated self-biased N-MOSFET is proposed and investigated by simulation. The device features an integrated self-biased N-MOSFET(ISM) on the anode active region. One side of the ISM is shorted to the P + anode electrode of RC-LIGBT and the other side is connected to the N + anode via a floating ohmic contact. The adaptively turn-on/off of the ISM contributes to improve the static and dynamic performance of the ISM RC-LIGBT. In the forward-state, due to the off-state of the ISM, the snapback could be effectively suppressed without requiring extra device area compared to the SSA (separated shorted anode) and STA (segmented trenches in the anode) LIGBTs. In the reverse conduction, the ISM is turned on and the parasitic NPN in the ISM is punched through, which provides a current path for the reverse current. Meanwhile, during the turn-off and reverse recovery states, the ISM turns on, providing a rapid electron extraction path. Thus, a superior tradeoff between the on-state voltage drop (V) and turnoff loss (E) as well as an improved reverse recovery characteristic can be obtained. Compared to the STA device, the proposed ISM RC-LIGBT reduces E by 21.5% without snapback. Its reverse recovery charge is reduced by 53.7%/58.6% compared to that of the SSA LIGBT with L = 40/60 μm at the same V. Due to the prominent static and dynamic characteristic, the power loss of ISM RC-LIGBT in a completed switching cycle is reduced.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s11671-022-03685-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9016105PMC
April 2022

CircTMEM59 Serves as miR-410-3p Sponge to Inhibit the Proliferation and Metastasis of Colorectal Cancer by Regulating HOXD8.

Biochem Genet 2022 Apr 15. Epub 2022 Apr 15.

Department of Inspection, 4th Affiliated Hospital of China Medical University, No. 4, Chongshan East Road, Huanggu District, Shenyang, 110032, China.

Circular RNAs (circRNAs) have been confirmed to be involved in the regulation of malignant progression of colorectal cancer (CRC). Our research aims to explore the role and mechanism of circTMEM59 (hsa_circ_0012634) in CRC progression. The expression of circTMEM59, miR-410-3p and Homeobox D8 (HOXD8) was measured by quantitative real-time PCR. Cell proliferation, migration and invasion were assessed by EdU assay, colony formation assay, wound healing assay and transwell assay. Marker protein expression was evaluated by western blot analysis. In addition, RNA interaction was confirmed by dual-luciferase reporter assay, RIP assay and RNA pull-down assay. CircTMEM59 was lowly expressed in CRC tumor tissues and cells. Upregulation of circTMEM59 repressed CRC cell proliferation, migration and invasion. MiR-410-3p could be sponged by circTMEM59, and its mimic also reversed the inhibition effect of circTMEM59 on CRC cell proliferation and metastasis. Also, HOXD8 was targeted by miR-410-3p, and its knockdown abolished the negative regulation of miR-410-3p inhibitor on CRC cell proliferation and metastasis. Furthermore, circTMEM59 inhibited CRC tumor growth by overexpressing HOXD8 through sponging miR-410-3p. In summary, our results demonstrated that circTMEM59 participated in the regulation of CRC progression through the miR-410-3p/HOXD8 axis, showing that circTMEM59 might be a potential target for the treatment of CRC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10528-022-10224-5DOI Listing
April 2022

Anti-hyperlipidemic and ameliorative effects of chickpea starch and resistant starch in mice with high fat diet induced obesity are associated with their multi-scale structural characteristics.

Food Funct 2022 May 10;13(9):5135-5152. Epub 2022 May 10.

College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an, Shaanxi, 710062, China.

Chickpea starches were isolated from both untreated (UC-S) and conventionally cooked seeds (CC-S), and their multi-scale structural characteristics and physiological effects on controlling hyperlipidemia in high fat diet induced obese mice were compared with their corresponding resistant starch (RS) fractions obtained by an enzymatic isolation method (UC-RS and CC-RS). The degree of order/degree of double helix in Fourier transform infrared spectroscopy was in the following order: CC-RS > UC-RS > CC-S > UC-S, which was consistent with the trend observed for relative crystallinity and double helix contents monitored by X-ray diffractometer and solid-state C cross-polarization and magic angle spinning NMR analyses. The influence of different types of chickpea starch and their corresponding resistant starch fractions on regulating the serum lipid profile, antioxidant status, and histopathological changes in liver, colon and cecal tissues, and gene expressions associated with lipid metabolism, gut microbiota, as well as short-chain fatty acid metabolites in mice with high fat diet induced obesity was investigated. The results showed that the chickpea RS diet group exhibited overall better anti-hyperlipidemic and ameliorative effects than those of the starch group, and such effects were most pronounced in the CC-RS intervention group. After a six-week period of administration with chickpea starch and RS diets, mice in the UC-RS and CC-RS groups tended to have relatively significantly higher levels ( < 0.05) of butyric acid in their fecal contents. The 16S rRNA sequencing results revealed that mice fed with CC-RS showed the greatest abundance of and compared with the other groups.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1fo04354dDOI Listing
May 2022

Significance of hyperhomocysteinaemia as an effective marker for vasculogenic erectile dysfunction: a cross-sectional study.

Transl Androl Urol 2022 Mar;11(3):397-406

Department of Urology and Andrology, Center for Reproductive Medicine, Cheeloo College of Medicine, Shandong University, Jinan, China.

Background: The primary objective of this cross-sectional study aimed at evaluating serum levels of homocysteinemia (Hcy) in a cohort of erectile dysfunction (ED) patients, and at assessing the correlation between Hcy and vasculogenic ED.

Methods: A total of 119 Chinese patients presenting with ED [International Index of Erectile Function (IIEF)-5≤21] were enrolled in this study. The whole cohort was asked to complete the IIEF-5 questionnaire and blood analysis. A detailed medical history, physical examination, nocturnal penile tumescence (NPT) tests and penile color Doppler ultrasonography (pDUS) were performed by a urologist. Based on the pDUS result, they were further categorized into 2 main groups: non-vasculogenic ED (n=79) and vasculogenic ED (n=40).

Results: Age and Hcy levels were significantly higher in the vasculogenic ED group compared to the non-vasculogenic ED group (38.45±8.51 32.29±7.57 and 22.91±5.85 16.31±5.23 µmol/L, respectively, P<0.01). Univariate and multivariable regression analyses showed that the incidence of vasculogenic ED raised significantly with the increase of Hcy level. After multivariable adjustment, ED patients with hyperhomocysteinaemia (HHcy) had 13.42 times the odds of vasculogenic ED compared with patients without HHcy (OR: 13.42, 95% CI: 3.78 to 47.64). Moreover, the risk of vasculogenic ED was 1.24 (95% CI: 1.04 to 1.48, P=0.01) per-unit increase in Hyc concentration for ED with HHcy. In addition, the smoothing curve revealed that Hcy levels were inversely correlated with peak-systolic velocity (PSV) (β: -0.48, 95% CI: -0.91 to -0.05, P=0.04) in vasculogenic ED patients by multivariate regression analysis. Furthermore, in the analysis of receiver operating characteristic curve, the area under curve for Hcy to predict vasculogenic ED was 0.81 (95% CI: 0.73 to 89) in patients with ED.

Conclusions: These findings suggest a dose-dependent association between Hcy and vasculogenic ED. HHcy was a risk factor for vasculogenic erectile dysfunction. Therefore, HHcy is likely to be a potential indicator to predict and diagnose vasculogenic ED when using pDUS.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.21037/tau-21-953DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8984975PMC
March 2022

LAMC1 is a Novel Prognostic Factor and a Potential Therapeutic Target in Gastric Cancer.

Int J Gen Med 2022 19;15:3183-3198. Epub 2022 Mar 19.

The Second Clinical Medical College, Lanzhou University, Lanzhou, Gansu, People's Republic of China.

Purpose: To investigate the role of LAMC1 in gastric cancer (GC), if it is of great importance to identify tumour driver genes with prognostic value.

Patients And Methods: GC-related gene expression profile data were downloaded from TCGA. R-limma package and univariate Cox regression were used to identify the differentially expressed genes (DEGs) and survival-genes, respectively. Then, the ClusterProfiler package was used to analyse the Gene Ontology and pathway enrichment of DEGs. Cytoscape was used to build a protein interaction network (PPI) and identify key genes. The GEPIA2 and TIMER databases were used to validate the differential expression of LAMC1. The relationship between LAMC1 and the prognosis of GC was analysed by the KM. GSEA and GSVA were used to analyse the major activated and mutated pathways, respectively. Real-time fluorescence quantitative PCR (RT-qPCR) was used to reidentify the expression of LAMC1 in GES-1 and 5 GC cell lines. Finally, we explored the relationship between LAMC1 and FGFR1.

Results: A total of 266 DEGs were be selected, which were mainly enriched in extracellular structure organization. LAMC1 was identified as one of the hub genes. The expression of LAMC1 was significantly higher in GC tissue than in paracancerous tissues, and the prognosis of the GC patient with high expression of LAMC1 was relatively poor. Univariate and multivariate Cox analysis indicated that LAMC1 could be used as an independent prognostic indicator. The results of GSEA and GSVA showed that LAMC1 was mainly enriched in pathways such as MYOGENESIS and UV_RESPONSE_DN. The RT-qPCR results showed that the expression level in AGS cells was significantly higher than that in gastric epithelial cells. LAMC1 may play a role in the development of gastric cancer by influencing FGFR1.

Conclusion: LAMC1 may mediate the occurrence and development of GC and has potential as a biomarker for the prognosis and treatment of GC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/IJGM.S353289DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8943981PMC
March 2022

Light plasticity of germination on the eastern Tibetan Plateau: Phylogeny, trait, and environmental correlates.

J Plant Physiol 2022 May 17;272:153670. Epub 2022 Mar 17.

Qinghai Provincial Key Laboratory of Restoration Ecology in Cold Regions, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai, 810008, China; Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai, 810008, China. Electronic address:

Seeds often exhibit great plasticity of germination in response to environmental variability and uncertainty. The causes of this plasticity, however, remain poorly understood, and comparative phylogenic analyses of such plasticity are rare. Here, we analyzed a field germination dataset including 474 species exposed to three different levels of light availability, using comparative phylogenetic methods. We calculated the plasticity of germination in response to light availability (PG) based on the maximum germination proportion (GP), PG based on the time required to reach 50% of GP, PG based on the relative germination velocity (RGV), and PG based on all three of these germination traits. We found that closely related species shared similar light plasticity of germination behavior. Different aspects of germination plasticity in response to light availability were related to specific traits or local environment. PG was associated with adult longevity and local water habitat, while PG was related to seed mass and local water habitat, and PG was marginally significantly related to plant height. PG was significantly associated with adult longevity and water habitat. These results suggested that different aspects of germination plasticity were located at specific niche dimension, and local habitats with sufficient soil moisture induced great plasticity germination in response to light environment. As such, they can simplify our understanding of germination, promote the exploration of the general law of germination, and further increase our understanding of species diversity maintenance, adaptation, and evolution from the perspective of germination.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jplph.2022.153670DOI Listing
May 2022

Evaluating the association between MPDZ-NF1B rs1324183 and keratoconus in an independent northwestern Chinese population.

BMC Ophthalmol 2022 Mar 19;22(1):129. Epub 2022 Mar 19.

Ningxia Clinical Research Center of Blinding Eye Disease, People Hospital of Ningxia Hui Autonomous Region (People's Hospital of Autonomous Region Affiliated to Ningxia Medical University), No 936, Huanghe East Road, Jinfeng District, Yinchuan, 750001, Ningxia, China.

Background: Keratoconus (KC) is a complex, non-inflammatory corneal degenerative disease. Although numerous studies have analyzed the correlation of SNP rs1324183, which located in MPDZ-NF1B gene, and KC in different populations, only few findings were repeated. In this study, to evaluate the association between rs1324183 and KC in a new independent Chinese population, we performed a replication study of the significantly associated rs1324183.

Methods: In total of 114 unrelated KC patients and 88 unrelated controls were recruited from Ningxia, China. We detected the genotypes and alleles of rs1324183 using PCR technology and Sanger sequencing and also analyzed the association between this locus and KC, its clinical parameters by statistical methods.

Results: The frequency of genotype AA (11, 9.6%) and genotypes containing allele A (47, 41.2%) of rs1324183 in KC were both higher than those of the control group. And genotype AA of rs1324183 conferred a higher risk of KC (OR > 1). Moreover, corneal parameter Belin/Ambrósio enhanced ectasia display final D value (BAD-D) had significant correlation (p = 0.002) with AA genotype of rs1324183 in KC.

Conclusions: Our replication study indicates that the results of rs1324183 associated with KC in our population is robust and further better illustrates the significance of BAD-D as a diagnostic indicator for KC. rs1324183 should be considered as the first genetic mark of KC risk in its future diagnosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12886-022-02359-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8934471PMC
March 2022

Diagnostic performance and interreader agreement of CEUS LI-RADS in ≤ 30 mm liver nodules with different experienced radiologists.

Abdom Radiol (NY) 2022 05 8;47(5):1798-1805. Epub 2022 Mar 8.

Department of Medical Ultrasound, The First Affiliated Hospital of Guangxi Medical University, No.6 Shuangyong Road, Nanning, 530021, Guangxi Zhuang Autonomous Region, China.

Purpose: To explore the diagnostic performance and interreader agreement of CEUS LI-RADS in diagnosing ≤ 30 mm liver nodules with different experienced radiologists.

Methods: Between January 2018 and October 2020, 244 patients at high-risk for HCC who underwent CEUS were enrolled. Two novice radiologists and two expert radiologists independently evaluated LI-RADS categories and main features. Kappa (κ) and Kendall's tests were employed to evaluate the interreader agreement of CEUS LI-RADS. The diagnostic performance was determined based on sensitivity, specificity, accuracy, PPV and NPV.

Results: The interreader agreement for arterial phase hyperenhancement, late and mild washout, early washout, and rim hyperenhancement was moderate to almost perfect (κ, 0.44-0.93) among the different levels of radiologists. The interreader agreement for the LI-RADS categories was substantial to almost perfect (κ, 0.78-0.88). However, the interreader agreement for marked washout was fair to moderate (κ, 0.28-0.50). When CEUS LR-5 was used as a diagnostic criterion for HCC, there were no statistical differences in sensitivity, specificity, accuracy, PPV and NPV among the radiologists (p > 0.05), except for the differences between Reader 4 and the remaining three radiologists in terms of accuracy and sensitivity (p < 0.05).

Conclusion: CEUS LI-RADS has good diagnostic agreement for ≤ 30 mm liver nodules among experienced radiologists.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00261-022-03468-7DOI Listing
May 2022

Characteristics of aerosol chemistry and acidity in Shanghai after PM satisfied national guideline: Insight into future emission control.

Sci Total Environ 2022 Jun 4;827:154319. Epub 2022 Mar 4.

Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP(3)), National Observations and Research Station for Wetland Ecosystems of the Yangtze Estuary, Department of Environmental Science and Engineering, Fudan University, Shanghai 200438, China; Institute of Eco-Chongming (IEC), Chongming District, Shanghai 202162, China.

With continuous endeavors to control air pollutant emissions, the average concentration of PM in Shanghai in 2019-2020 satisfied the national secondary standard (35 μg m) for the first time. In this study, the two-year dataset of hourly resolution PM compositions observed in downtown Shanghai was used to investigate the relative contribution of sulfate and nitrate as well as particulate acidity. The average concentration of SO was reduced to 7.7 μg m, while the concentration of NO remained above 40 μg m, indicating that the control of SO was more effective than that of NO during the 13th Five-Year Plan period. Thus, the sulfate pollution was significantly reduced whereas the nitrate loading remained almost constant. The monthly N/S ratio varied from below 0.6 to above 2.0, indicating that the contribution of automobile exhaust to PM is seasonally dependent. Contrary to sulfate, the nitrate fraction increased rapidly with the increase of PM mass, suggesting that the explosive growth of nitrate has become a major driver of haze formation. ISORROPIA simulations show that PM was moderately acidic with pH values following the trend of winter > spring > autumn > summer. The diurnal variation of nitrate was related to the changes in aerosol water content, indicating the effect of heterogeneous aqueous reactions on secondary aerosol formation. The effectiveness of emission control for reducing inorganic PM varied with different gas precursors and seasons. The abatement of NH emissions will increase particle acidity and acid rain pollution, although it is more effective than that of NO when the emission reduction is larger than 60%. This study suggests that the control of vehicle exhaust should be given priority in the Yangtze River Delta for coordinately mitigating PM and acid rain pollution.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2022.154319DOI Listing
June 2022

The fiber diameter traits of Tibetan cashmere goats are governed by the inherent differences in stress, hypoxic, and metabolic adaptations: an integrative study of proteome and transcriptome.

BMC Genomics 2022 Mar 7;23(1):191. Epub 2022 Mar 7.

Key Laboratory of Genetics Breeding and Reproduction of the Wool Sheep & Cashmere Goat in Xinjiang, Institute of Animal Science, Xinjiang Academy of Animal Sciences, Urumqi, China.

Background: Tibetan cashmere goats are served as a valuable model for high altitude adaptation and hypoxia complications related studies, while the cashmere produced by these goats is an important source of income for the herders. The aim of this study was to investigate the differences in protein abundance underlying the fine (average 12.20 ± 0.03 μm of mean fiber diameter) and coarse cashmere (average 14.67 ± 0.05 μm of mean fiber diameter) producing by Tibetan cashmere goats. We systematically investigated the genetic determinants of fiber diameter by integrated analysis with proteomic and transcriptomic datasets from skin tissues of Tibetan cashmere goats.

Results: We identified 1980 proteins using a label-free proteomics approach. They were annotated to three different databases, while 1730 proteins were mapped to the original protein coding genes (PCGs) of the transcriptomic study. Comparative analyses of cashmere with extremely fine vs. coarse phenotypes yielded 29 differentially expressed proteins (DEPs), for instance, APOH, GANAB, AEBP1, CP, CPB2, GPR142, VTN, IMPA1, CTSZ, GLB1, and HMCN1. Functional enrichment analysis of these DEPs revealed their involvement in oxidation-reduction process, cell redox homeostasis, metabolic, PI3K-Akt, MAPK, and Wnt signaling pathways. Transcription factors enrichment analysis revealed the proteins mainly belong to NF-YB family, HMG family, CSD family. We further validated the protein abundance of four DEPs (GC, VTN, AEBP1, and GPR142) through western blot, and considered they were the most potential candidate genes for cashmere traits in Tibetan cashmere goats.

Conclusions: These analyses indicated that the major biological variations underlying the difference of cashmere fiber diameter in Tibetan cashmere goats were attributed to the inherent adaptations related to metabolic, hypoxic, and stress response differences. This study provided novel insights into the breeding strategies for cashmere traits and enhance the understanding of the biological and genetic mechanisms of cashmere traits in Tibetan cashmere goats.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12864-022-08422-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8903710PMC
March 2022

Controlling Mesenchyme Tissue Remodeling via Spatial Arrangement of Mechanical Constraints.

Front Bioeng Biotechnol 2022 18;10:833595. Epub 2022 Feb 18.

Department of Biomedical and Chemical Engineering, Syracuse University, Syracuse, NY, United States.

Tissue morphogenetic remodeling plays an important role in tissue repair and homeostasis and is often governed by mechanical stresses. In this study, we integrated an mesenchymal tissue experimental model with a volumetric contraction-based computational model to investigate how geometrical designs of tissue mechanical constraints affect the tissue remodeling processes. Both experimental data and simulation results verified that the standing posts resisted the bulk contraction of the tissues, leading to tissue thinning around the posts as gap extension and inward remodeling at the edges as tissue compaction. We changed the geometrical designs for the engineered mesenchymal tissues with different shapes of posts arrangements (triangle vs. square), different side lengths (6 mm vs. 8 mm), and insertion of a center post. Both experimental data and simulation results showed similar trends of tissue morphological changes of significant increase of gap extension and deflection compaction with larger tissues. Additionally, insertion of center post changed the mechanical stress distribution within the tissues and stabilized the tissue remodeling. This experimental-computational integrated model can be considered as a promising initiative for future mechanistic understanding of the relationship between mechanical design and tissue remodeling, which could possibly provide design rationale for tissue stability and manufacturing.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fbioe.2022.833595DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8896258PMC
February 2022

Genome-wide comparative analysis of DNAJ genes and their co-expression patterns with HSP70s in aestivation of the sea cucumber Apostichopus japonicus.

Funct Integr Genomics 2022 Jun 23;22(3):317-330. Epub 2022 Feb 23.

Dalian Ocean University, Dalian, 116023, Liaoning, China.

DNAJ proteins function as co-chaperones of HSP70 and play key roles in cell physiology to promote protein folding and degradation, especially under environmental stress. Based on our previous study on HSP70, a systematic study of DNAJ was performed in sea cucumber Apostichopus japonicus using the transcriptomic and genomic data, identifying 43 AjDNAJ genes, including six AjDNAJA genes, eight AjDNAJB genes, and 29 AjDNAJC genes. Slight expansion and conserved genomic structure were observed using the phylogenetic and syntenic analysis. Differential period-specific and tissue-specific expression patterns of AjDNAJs were observed between adult and juvenile individuals during aestivation. Strong tissue-specific expression correlations between AjDNAJ and AjHSP70 genes were found, indicating that the involvements of AjHSP70IVAs in the aestivation of sea cucumbers were regulated by AjDNAJs. Several key genes with significant expression correlations, such as AjDNAJB4L and AjHSP70IVAs, were suggested to function together under heat stress. Together, these findings provide early insight into the involvement of AjDNAJs in the aestivation and their roles as co-chaperones of AjHSP70s.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10142-022-00830-2DOI Listing
June 2022

Ni-Modified Ag/SiO Catalysts for Selective Hydrogenation of Dimethyl Oxalate to Methyl Glycolate.

Nanomaterials (Basel) 2022 Jan 26;12(3). Epub 2022 Jan 26.

Department of Environmental Science and Engineering, Fudan University, Shanghai 200438, China.

Ni-modified Ag/SiO catalysts containing 0~3 wt.% Ni were obtained by impregnating Ni species onto Ag/SiO followed by calcination and reduction. The catalysts' performance in the hydrogenation of dimethyl oxalate (DMO) to methyl glycolate (MG) was tested. Ag-0.5%Ni/SiO showed the highest catalytic activity among these catalysts and exhibited excellent catalytic stability. The effects of the Ni content on the structure and surface chemical states of catalysts were investigated by XRF, N-sorption, XRD, TEM, EDX-mapping, FT-IR, H-TPR, UV-vis, and XPS. The better catalytic activity and stability of Ni-modified Ag/SiO (versus Ag/SiO) are ascribed to the improved dispersion of active Ag species as well as the higher resistance to the growth of Ag particles due to the presence of Ni species.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/nano12030407DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8838820PMC
January 2022

Life cycle assessment of tiger puffer (Takifugu rubripes) farming: A case study in Dalian, China.

Sci Total Environ 2022 Jun 29;823:153522. Epub 2022 Jan 29.

Key Laboratory of Environment Controlled Aquaculture (Dalian Ocean University) Ministry of Education, 52 Heishijiao Street, Dalian 116023, PR China; College of Marine Technology and Environment, Dalian Ocean University, 52 Heishijiao Street, Dalian 116023, PR China; College of Biosystems Engineering and Food Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, PR China. Electronic address:

In China, energy consumption and carbon emission by the aquaculture industry have become major problems. The tiger puffer (Takifugu rubripes) is an emerging aquaculture species in China, but its environmental impact during the farming process has not yet been evaluated systematically. To the best of our knowledge, this is the first life cycle assessment (LCA) of tiger puffer land-sea relay strategy in Dalian, China. To analyze the environmental impact of the tiger puffer farming process, the following four stages were considered: seed rearing, deep-sea cage farming-1, industrial recirculating aquaculture, and deep-sea cage farming-2. The LCA software GaBi 10.5 academy version and CML-IA-Jan. 2016-world method were used to calculate the environmental impacts. According to the LCA results, marine aquatic ecotoxicity potential was the largest contributor to the environmental impact, and industrial recirculating aquaculture was the largest farming stage in the whole tiger puffer farming process. Energy in the form of electricity, coal, and gasoline was consumed to maintain the power supply in the tiger puffer farming process, and it was a key factor that influenced the environmental performance. Based on the sensitivity and energy analyses, energy consumption for equipment operation at the industrial recirculating aquaculture stage, feed consumption, and gasoline consumption for transportation at the deep-sea cage farming-2 stage need to be carefully considered. The following improvement measures were suggested to improve the environmental performance of tiger puffer farming and the aquaculture industry: establish electricity, wind power, and solar energy integrated management systems; ex-ante LCA for parameter optimization in future technology research and development; and new production strategies such as aquaponics and integrated multi-trophic aquaculture. Moreover, life cycle inventory (LCI) of tiger puffer land-sea relay farming was established to obtain essential information, enrich aquaculture LCI databases, and support aquaculture LCA research.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2022.153522DOI Listing
June 2022

Circ_0009910 Serves as miR-361-3p Sponge to Promote the Proliferation, Metastasis, and Glycolysis of Gastric Cancer via Regulating SNRPA.

Biochem Genet 2022 Jan 31. Epub 2022 Jan 31.

Department of Gastrointestinal Surgery, Hubei Xiantao First People's Hospital, Xiantao, Hubei, China.

Circular RNA (circRNA) has been proved to be a key regulator of gastric cancer (GC) progression. Circ_0009910 was found to be highly expressed in GC and related to GC progression, but its role and mechanism in GC progression need to be further improved. Our study aims to further reveal circ_0009910 roles in GC progression and elucidate its potential molecular mechanism. The expression of circ_0009910, microRNA (miR)-361-3p, and small nuclear ribonucleoprotein polypeptide A (SNRPA) mRNA was measured by quantitative real-time PCR. Protein expression was determined using western blot analysis. Cell proliferation, migration, invasion, and apoptosis were evaluated using EDU staining, transwell assay, and flow cytometry. Cell glycolysis were assessed by detecting glucose consumption, lactate production, and glycolysis-related markers protein expression. The relationship between miR-361-3p and circ_0009910 or SNRPA was confirmed by RNA pull-down assay and dual-luciferase reporter assay. In vivo experiments were performed to explore the effect of circ_0009910 silencing on GC tumorigenesis. Circ_0009910 and SNRPA were upregulated in GC tumor tissues and cells. Knockdown of circ_0009910 or SNRPA could inhibit GC cell proliferation, migration, invasion, glycolysis, and promote apoptosis. Circ_0009910 could sponge miR-361-3p, and miR-361-3p could target SNRPA. Further experiments confirmed that circ_0009910 positively regulated SNRPA by sponging miR-361-3p. Additionally, SNRPA overexpression abolished the negative regulation of circ_0009910 silencing on GC progression. Furthermore, silenced circ_0009910 also reduced GC tumorigenesis in vivo. Our data showed that circ_0009910 might be a target for GC treatment, which could promote GC proliferation, metastasis, and glycolysis by the miR-361-3p/SNRPA axis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10528-021-10168-2DOI Listing
January 2022

Spatiotemporal Dynamics of the Carbon Budget and the Response to Grazing in Qinghai Grasslands.

Front Plant Sci 2021 7;12:775015. Epub 2022 Jan 7.

Key Laboratory of Restoration Ecology for Cold Regions Laboratory in Qinghai, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, China.

Estimating the grassland carbon budget is critically important for ensuring that grassland resources are used sustainably. However, the spatiotemporal dynamics of the carbon budget and the response to grazing have not yet been characterized in Qinghai grasslands. Here, we estimated the gross primary productivity (GPP) and net ecosystem exchange (NEE) in Qinghai grasslands using the improved Biome-BGCMuSo model to characterize the spatiotemporal dynamics of the carbon budget and the response to grazing in this region from 1979 to 2018. The GPP of Qinghai grasslands fluctuated during the study period, with an average annual value of 118.78 gC/m. The NEE of Qinghai grasslands fluctuated from 1979 to 2018, with an average value of -5.16 gC/m. After 2,000, GPP increased, and NEE decreased in a fluctuating manner. There were clear regional differences in GPP and NEE. GPP was low in most areas of Qinghai, and GPP was high in eastern and southern Qinghai. The southern, southeastern, and northeastern parts of Qinghai were mainly carbon sinks, and the northwestern part of Qinghai and the region between the southeastern and northeastern parts of Qinghai were mainly carbon sources. Grazing generally decreased GPP and increased NEE in Qinghai grasslands from 1979 to 2018. There was spatial heterogeneity in the effect of grazing on GPP and NEE. Under grazing, GPP and NEE were significantly decreased mainly in eastern Qinghai, and GPP and NEE were significantly increased mainly in southern and eastern Qinghai. NEE was most affected by grazing in eastern Qinghai. The results of this study aid our understanding of the mechanism driving variation in the grassland carbon budget and provide new data that could be used to support local grassland management.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpls.2021.775015DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8777210PMC
January 2022

Controlling Morphology and Functions of Cardiac Organoids by Two-Dimensional Geometrical Templates.

Cells Tissues Organs 2022 Jan 10. Epub 2022 Jan 10.

Traditionally, tissue-specific organoids are generated as 3D aggregates of stem cells embedded in Matrigel or hydrogels, and the aggregates eventually end up a spherical shape and suspended in the matrix. Lack of geometrical control of organoid formation makes these spherical organoids limited for modeling the tissues with complex shapes. To address this challenge, we developed a new method to generate 3D spatial-organized cardiac organoids from 2D micropatterned hiPSC colonies, instead of directly from 3D stem cell aggregates. This new approach opens the possibility to create cardiac organoids that are templated by 2D non-spherical geometries, which potentially provides us a deeper understanding of biophysical controls on developmental organogenesis. Here, we designed 2D geometrical templates with quadrilateral shapes and pentagram shapes that had same total area but different geometrical shapes. Using this templated substrate, we grew cardiac organoids from human induced pluripotent stem cells (hiPSCs) and collected a series of parameters to characterize morphological and functional properties of the cardiac organoids. In quadrilateral templates, we found that increasing the aspect ratio impaired cardiac tissue 3D self-assembly, but the elongated geometry improved the cardiac contractile functions. However, in pentagram templates, cardiac organoid structure and function were optimized with a specific geometry of an ideal star shape. This study will shed a light on "organogenesis-by-design" by increasing the intricacy of starting templates from external geometrical cues to improve the organoid morphogenesis and functionality.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1159/000521787DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9271134PMC
January 2022

Mucinous Ovarian Tumors With Anaplastic Mural Nodules: Case Report.

Front Med (Lausanne) 2021 14;8:753904. Epub 2021 Dec 14.

Department of Obstetrics and Gynecology, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China.

Ovarian mucinous cystic tumors may be associated with various types of mural nodules, which can be classified as benign or malignant (anaplastic carcinoma, sarcoma, carcinosarcoma). However, anaplastic malignant nodules have rarely been reported. Here, we present a case of a 35-year-old woman who presented with abdominal discomfort. Ultrasonography showed a large cystic mass in the pelvic and abdominal cavities measuring 337 × 242 mm. Abdominal computed tomography revealed upper anterior and posterior uterine pelvic cystic lesions based on multiple nodule partition walls and classes. During hospitalization, the patient underwent exploratory laparotomy, which revealed a poorly differentiated ovarian malignant tumor, and subsequent surgical excision was performed. The pathological analysis of the surgical samples of the right ovary revealed a mucinous ovarian tumor, while the mural nodules were classified as anaplastic carcinoma. After surgery, the patient started receiving chemotherapy. Unfortunately, the patient died 6 months later. Mucinous tumor occurring with an anaplastic carcinoma is rare, and the current diagnostic methods are not sufficient in providing an early and accurate diagnosis. Most patients are already in the advanced stage upon diagnosis and combined with poorly differentiated pathological features, the prognosis is extremely poor. Clinicians need to improve the clinical evaluation before surgery and conduct preoperative preparation and communication to improve the prognosis of patients as much as possible.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmed.2021.753904DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8712860PMC
December 2021

Genome-wide identification and analysis of TMT-based proteomes in longissimus dorsi tissue from Kazakh cattle and Xinjiang brown cattle.

Anim Biotechnol 2021 Dec 30:1-12. Epub 2021 Dec 30.

School of Animal Science and Technology, Shihezi University, Shihezi, China.

With the gradual completion of the human genome project, proteomes have gained extremely important value in the fields of human disease and biological process research. In our previous research, we performed transcriptomic analyses of longissimus dorsi tissue from Kazakh cattle and Xinjiang brown cattle and conducted in-depth studies on the muscles of both species through epigenetics. However, it is unclear whether differentially expressed proteins in Kazakh cattle and Xinjiang brown cattle regulate muscle production and development. In this study, a proteomic analysis was performed on Xinjiang brown cattle and Kazakh cattle by using TMT markers, HPLC classification, LC/MS and bioinformatics analysis. A total of 13,078 peptides were identified, including 11,258 unique peptides. We identified a total of 1874 proteins, among which 1565 were quantifiable. Compared to Kazakh cattle, Xinjiang brown cattle exhibited 75 upregulated proteins and 44 downregulated proteins. These differentially expressed proteins were enriched for the functions of adrenergic signaling in cardiomyocytes, fatty acid degradation and glutathione metabolism. In our research, we found differentially expressed proteins in longissimus dorsi tissue between Kazakh cattle and Xinjiang brown cattle. We predict that these proteins regulate muscle production and development through select enriched signaling pathways. This study provides novel insights into the roles of proteomes in cattle genetics and breeding.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/10495398.2021.2019756DOI Listing
December 2021
-->