Publications by authors named "Zhen Liu"

2,006 Publications

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Increased expression of cyclooxygenase-2 in synovium tissues and synovial fluid from patients with knee osteoarthritis is associated with downregulated microRNA-758-3p expression.

Exp Ther Med 2021 Sep 15;22(3):1001. Epub 2021 Jul 15.

Department of Hand and Foot Microsurgery, Heze Municipal Hospital, Heze, Shandong 274000, P.R. China.

Cyclooxygenase-2 (COX-2) is a common factor in inflammation, and its specific regulatory mechanism has not been fully elucidated. The present study aimed to investigate COX-2 mRNA and protein expression levels in synovium tissues and synovial fluid from patients with knee osteoarthritis (KOA), and determine the molecular mechanism by which microRNA (miRNA/miR)-758 regulates KOA via COX-2. A total of 37 patients with KOA and 29 patients with acute knee trauma (control group) were enrolled in the present study. Reverse transcription-quantitative PCR analysis was performed to detect miR-758-3p and COX-2 mRNA expression, while western blotting and ELISA were performed to detect COX-2 protein expression in synovium and synovial fluid, respectively. The dual-luciferase reporter assay was performed to verify the interaction between miR-758-3p and the 3'-untraslated region (UTR) of COX-2 mRNA. Synovial cells were transfected with agomiR-758-3p, and the MTT assay was performed to assess cell proliferation. The results demonstrated that COX-2 expression was higher in patients with KOA than those with acute knee trauma. Conversely, miR-758-3p expression was lower in patients with KOA than those with acute knee trauma. Notably, miR-758-3p interacted with the 3'-UTR of COX-2 mRNA to regulate its expression. Overexpression of miR-758-3p inhibited the expression and release of COX-2, as well as the proliferation of human KOA synovial cells. Taken together, these results suggest that COX-2 expression is upregulated in synovium tissues and synovial fluid from patients with KOA, which is associated with downregulated miR-758-3p expression. In addition, miR-758-3p affects the proliferation of synovial cells and the expression of relevant proteins in these cells, thus promoting the occurrence and development of KOA.
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http://dx.doi.org/10.3892/etm.2021.10433DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8311242PMC
September 2021

RUNX3 Expression Level Is Correlated with the Clinical and Pathological Characteristics in Endometrial Cancer: A Systematic Review and Meta-analysis.

Biomed Res Int 2021 14;2021:9995384. Epub 2021 Jul 14.

Geneis (Beijing) Co. Ltd., Beijing 100102, China.

Human Runt-associated transcription factor 3 (RUNX3) plays an important role in the development and progression of endometrial cancer (EC). However, the clinical and pathological significance of RUNX3 in EC needs to be further studied. In order to clarify the clinical and pathological significance of RUNX3, a systematic review and meta-analysis was conducted in EC patients. Keywords RUNX3, endometrial cancer, and uterine cancer were searched in Cochrane Library, Web of Knowledge, PubMed, CBM, MEDLINE, and Chinese CNKI database for data up to Dec 31, 2018. References, abstracts, and meeting proceedings were manually searched in supplementary. Outcomes were various clinical and pathological features. The two reviewers performed the literature searching, data extracting, and method assessing independently. Meta-analysis was performed by RevMan5.3.0. A total of 563 EC patients were enrolled from eight studies. Meta-analysis results showed that the expression of RUNX3 has significant differences in these comparisons: lymph node (LN) metastasis vs. non-LN metastasis ( = 0.26), EC tissues vs. normal tissues ( < 0.00001), clinical stages I/II vs. II/IV ( < 0.00001), muscular infiltration < 1/2 vs. muscular infiltration ≥ 1/2 ( < 0.00001), and G1 vs. G2/G3 ( < 0.00001). The decreasing expression of RUNX3 is associated with poor TNM stage and muscular infiltration. It is indicated that RUNX3 was involved in the suppression effect of EC. However, further multicenter randomized controlled trials are needed considering the small sample size of the included trials.
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http://dx.doi.org/10.1155/2021/9995384DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8298141PMC
July 2021

CCDC65 as a new potential tumor suppressor induced by metformin inhibits activation of AKT1 via ubiquitination of ENO1 in gastric cancer.

Theranostics 2021 13;11(16):8112-8128. Epub 2021 Jul 13.

Cancer Center, Integrated Hospital of Traditional Chinese Medicine, Southern Medical University, 510315 Guangzhou, China.

The coiled-coil domain containing protein members have been well documented for their roles in many diseases including cancers. However, the function of the coiled-coil domain containing 65 (CCDC65) remains unknown in tumorigenesis including gastric cancer. CCDC65 expression and its correlation with clinical features and prognosis of gastric cancer were analyzed in tissue. The biological role and molecular basis of CCDC65 were performed via in vitro and in vivo assays and a various of experimental methods including co-immunoprecipitation (Co-IP), GST-pull down and ubiquitination analysis et al. Finally, whether metformin affects the pathogenesis of gastric cancer by regulating CCDC65 and its-mediated signaling was investigated. : Here, we found that downregulated CCDC65 level was showed as an unfavourable factor in gastric cancer patients. Subsequently, CCDC65 or its domain (a.a. 130-484) was identified as a significant suppressor in GC growth and metastasis in vitro and in vivo. Molecular basis showed that CCDC65 bound to ENO1, an oncogenic factor has been widely reported to promote the tumor pathogenesis, by its domain (a.a. 130-484) and further promoted ubiquitylation and degradation of ENO1 by recruiting E3 ubiquitin ligase FBXW7. The downregulated ENO1 decreased the binding with AKT1 and further inactivated AKT1, which led to the loss of cell proliferation and EMT signal. Finally, we observed that metformin, a new anti-cancer drug, can significantly induce CCDC65 to suppress ENO1-AKT1 complex-mediated cell proliferation and EMT signals and finally suppresses the malignant phenotypes of gastric cancer cells. These results firstly highlight a critical role of CCDC65 in suppressing ENO1-AKT1 pathway to reduce the progression of gastric cancer and reveals a new molecular mechanism for metformin in suppressing gastric cancer. Our present study provides a new insight into the mechanism and therapy for gastric cancer.
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http://dx.doi.org/10.7150/thno.54961DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8315052PMC
July 2021

The effects of miR-26b-5p on fibroblast-like synovial cells in rheumatoid arthritis (RA-FLS) via targeting EZH2.

Tissue Cell 2021 Jul 15;72:101591. Epub 2021 Jul 15.

Department of Ultrasound, Renmin Hospital, Hubei University of Medicine, Shiyan, Hubei, 442000, China. Electronic address:

Objective: To study the possible effects of miR-26b-5p on fibroblast-like synovial cells in rheumatoid arthritis (RA-FLS) through targeting enhancer of zeste homolog 2 (EZH2).

Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect miR-26b-5p and EZH2 expressions in synovial tissues of RA patients and healthy controls. Dual luciferase reporter assay was adopted to verify the targeting relationship between miR-26b-5p and EZH2. RA-FLS was divided into Blank, mimics NC, mimics, NC siRNA, EZH2 siRNA and inhibitors + EZH2 siRNA groups, followed by the assessment of proliferation, apoptosis, migration and invasion. The expression of genes and proteins in RA-FLS was tested by qRT-PCR and western blotting, respectively.

Results: MiR-26b-5p expression was lower, while EZH2 expression was higher in synovial tissue of RA patients than healthy controls; and miR-26b-5p was negatively correlated with the EZH2 in synovial tissue of RA patients, which were both related with disease activities. MiR-26b-5p can target EZH2 in RA-FLS. In vitro, miR-26b-5p mimics down-regulated EZH2 expression in RA-FLS. Compared with EZH2 siRNA group, the miR-26b-5p expression in inhibitors + EZH2 siRNA group was reduced, but EZH2 expression was increased. EZH2 siRNA inhibited the proliferation, invasion and migration of RA-FLS, promoted cell apoptosis, and inhibited the expression of TNF-α, IL-1β, IL-6, IL-17, MMP-2, MMP-9, which were reversed by miR-26b-5p inhibitor.

Conclusion: MiR-26b-5p may affect the biological characteristics of RA-FLS via targeting EZH2, including proliferation, apoptosis, invasion and migration, as well as the secretion of cytokines, thus playing a potential therapeutic role in RA.
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http://dx.doi.org/10.1016/j.tice.2021.101591DOI Listing
July 2021

Salivary High-Risk Human Papillomavirus DNA as a Biomarker for Human Papillomavirus-Driven Head and Neck Cancers.

J Mol Diagn 2021 Jul 26. Epub 2021 Jul 26.

Saliva & Liquid Biopsy Translational Laboratory, School of Biomedical Science, Faculty of Health, Queensland University of Technology (QUT), Queensland, Australia; Translational Research Institute, Queensland, Australia. Electronic address:

High-risk human papillomavirus (HR-HPV) infection is one of the major risk factors for head and neck cancers (HNC). Despite the rising incidence, biomarkers for detection, prognostication and disease monitoring of HPV-driven HNC (HPV-HNC) are lacking. To evaluate the capacity of salivary HR-HPV DNA as a biomarker for HPV-HNC, salivary HR-HPV status of 491 primary HNC patients and 10 recurrent HNC patients was investigated at diagnosis using qPCR and MassARRAY. Tumor Cyclin-dependent kinase inhibitor 2A (p16) expression was determined by immunohistochemistry. Oropharyngeal cancer (OPC) patients (N=215) were followed-up up to 5 years and survival characteristics were evaluated in terms of event-free survival (EFS) and cause-specific survival (CSS). In the primary HNC patient cohort, 43.2% were positive for salivary HR-HPV DNA and the vast majority of them had OPC. Salivary HR-HPV DNA could be detected in 81.4% of tumor p16 positive OPC patients at diagnosis. Furthermore, salivary HR-HPV positive OPC patients had a favorable prognosis over salivary HR-HPV negative patients (EFS; HR: 0.42; 95%CI, 0.21-0.81, p=0.010, CSS; HR: 0.39; 95%CI, 0.18-0.86, p=0.019). In the recurrent HNC cohort, salivary HR-HPV DNA was detected in 83.3% (5/6) of the patients who previously had tumor p16 positive HNC. Findings of this study indicate that this liquid biopsy-based, non-invasive biomarker can play an essential role in the detection and management of HPV-HNC.
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http://dx.doi.org/10.1016/j.jmoldx.2021.07.005DOI Listing
July 2021

Real-time confocal microscopy imaging of corneal cytoarchitectural changes induced by different stresses.

Exp Eye Res 2021 Jul 26;210:108706. Epub 2021 Jul 26.

Eye Institute & Affiliated Xiamen Eye Center, School of Pharmaceutical Sciences & School of Medicine, Xiamen University, Xiamen, China; Fujian Provincial Key Laboratory of Ophthalmology and Visual Science, Xiamen, China. Electronic address:

Maintenance of the corneal refractive power and tissue transparency is essential for normal vision. Real-time characterization of changes in corneal cells during suffering stresses or wound healing may provide a way to identify novel targets, whose therapeutic manipulation can improve the outcome of this response induced by injury. Here we describe a novel user friendly and effective confocal real-time confocal microscopy attachment that monitors the effects of anisoosmotic stress on cell morphology and corneal thickness in situ. Corneal epithelial nuclei gradually became highly reflective in the isotonic group and the corneal stroma was slightly thickened as compared with that seen prior to 60 min exposure to a hypotonic solution. After 30 min of exposure to hypertonic stress, the corneal stromal cells became crenate and shriveled. The hyper-reflective area of the corneal stroma in the hypo-osmotic group was significantly larger than that in the other two groups, as demonstrated by 3D reconstruction imaging. The hypotonic fresh chlorinated pool water was observed to cause atrophy of corneal epithelial nuclei, while the isosmotic bee venom solution caused high reflection of the corneal stroma layer and corneal endothelial cell damage. With the microscopic attachment, the inward movement of corneal epithelial cells toward the denuded central region was detected in the serum-treated group. The microscopy attachment is an effective system for obtaining a more detailed understanding of the time dependent losses in the corneal cell structure and tissue architecture of full thickness corneas induced by osmotic stress or cytotoxic agents.
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http://dx.doi.org/10.1016/j.exer.2021.108706DOI Listing
July 2021

SIMPLE is an endosomal regulator that protects against non-alcoholic fatty liver disease by targeting the lysosomal degradation of EGFR.

Hepatology 2021 Jul 28. Epub 2021 Jul 28.

Department of Cardiology, State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Background & Aims: Nonalcoholic fatty liver disease (NAFLD) has become a tremendous burden for public health, however, there is no drug for NAFLD therapy at present. Impaired endo-lysosome-mediated protein degradation is observed in a variety of metabolic disorders, such as atherosclerosis, type 2 diabetes mellitus and NAFLD. Small integral membrane protein of lysosome/late endosome (SIMPLE) is a regulator of endosome-to-lysosome trafficking and cell signaling. But the role that SIMPLE plays in NAFLD progression remains unknown. Here we investigated SIMPLE function in NAFLD development and sophisticated mechanism therein.

Approach & Results: This study found in vitro knockdown of SIMPLE significantly aggravated lipid accumulation, inflammation in hepatocytes treated with metabolic stimulation. Consistently, in vivo experiments showed that liver-specific Simple-knockout (Simple-HKO) mice exhibited more severe high-fat diet (HFD)-, high-fat-high-cholesterol diet (HFHC)-, and methionine-choline-deficient diet (MCD)- induced steatosis, glucose intolerance, inflammation, and fibrosis than those fed with normal-chow diet. Meanwhile, RNA-sequencing demonstrated the up-regulated signaling pathways and signature genes involved in lipid metabolism, inflammation and fibrosis in Simple-HKO mice compared to control mice under metabolic stress. Mechanically, we found SIMPLE directly interact with epidermal growth factor receptor (EGFR). SIMPLE deficiency results in dysregulated degradation of EGFR, subsequently hyperactivated EGFR phosphorylation, exaggerating NAFLD development. Moreover, we further demonstrated that using EGFR inhibitor or silencing EGFR expression could ameliorate lipid accumulation induced by the knockdown of SIMPLE.

Conclusions: SIMPLE ameliorated NASH by prompting EGFR degradation and can also be a potential therapeutic candidate for NASH.
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http://dx.doi.org/10.1002/hep.32075DOI Listing
July 2021

Effects of Electroacupuncture on Sleep via the Dopamine System of the HPA Axis in Rats after Cage Change.

Evid Based Complement Alternat Med 2021 1;2021:5527060. Epub 2021 Jul 1.

Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China.

Background: Insomnia is often related to stressful events. The hypothalamus-pituitary-adrenal (HPA) axis is related to stress, and dopamine (DA) and DA receptors are involved in the regulation of HPA axis. Electroacupuncture (EA) can improve sleep in individuals with insomnia, but the mechanism is unclear. We demonstrated that EA can improve sleep in rats after cage change through DA and the DA receptors in the HPA axis.

Methods: A rat model of insomnia was established by cage change to a dirty cage. The rats in treatment groups were intervened by EA and D1R (or D2R) antagonists. Electroencephalography (EEG) and electromyogram (EMG) were recorded to compare the changes in sleep. The DA, corticotropin-releasing hormone (CRH), adrenocorticotropic hormone (ACTH), and cortisol (CORT) levels in the plasma and hypothalamus were measured by ELISAs, and the D1R and D2R levels were measured by RT-PCR and immunohistochemistry.

Results: The dirty group showed a significant increase in the amount of wakefulness and decrease in the amount of NREM sleep, with decreased numbers of long NREM sleep bouts and REM sleep bouts and increased mean duration of wakefulness during the light period. EA and D1R (or D2R) antagonists intervention could improve sleep disturbance by decreasing wakefulness in the light period after cage change, EA and D1R (or D2R) antagonists could increase the hypothalamus DA, CRH, ACTH, CORT level, and the D1R and D2R mRNA levels in the HPA axis, and the effect of EA plus D1R (or D2R) antagonist was not superior to that of EA or D1R (or D2R) antagonists alone.

Conclusions: EA can improve the sleep of rats after cage change, and the mechanism may be related to the regulation of DA and D1R or D2R in the HPA axis.
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http://dx.doi.org/10.1155/2021/5527060DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8270700PMC
July 2021

Real-Time Structural Monitoring of the Multi-Point Hoisting of a Long-Span Converter Station Steel Structure.

Sensors (Basel) 2021 Jul 11;21(14). Epub 2021 Jul 11.

School of Civil Engineering, Sun Yat-sen University, No.135 XinGangXiLu, Guangzhou 510275, China.

In the process of using a long-span converter station steel structure, engineering disasters can easily occur. Structural monitoring is an important method to reduce hoisting risk. In previous engineering cases, the structural monitoring of long-span converter station steel structure hoisting is rare. Thus, no relevant hoisting experience can be referenced. Traditional monitoring methods have a small scope of application, making it difficult to coordinate monitoring and construction control. In the monitoring process, many problems arise, such as complicated installation processes, large-scale data processing, and large-scale installation errors. With a real-time structural monitoring system, the mechanical changes in the long-span converter station steel structure during the hoisting process can be monitored in real-time in order to achieve real-time warning of engineering disasters, timely identification of engineering issues, and allow for rapid decision-making, thus avoiding the occurrence of engineering disasters. Based on this concept, automatic monitoring and manual measurement of the mechanical changes in the longest long-span converter station steel structure in the world is carried out, and the monitoring results were compared with the corresponding numerical simulation results in order to develop a real-time structural monitoring system for the whole long-span converter station steel structure's multi-point lifting process. This approach collects the monitoring data and outputs the deflection, stress, strain, wind force, and temperature of the long-span converter station steel structure in real-time, enabling real-time monitoring to ensure the safety of the lifting process. This research offers a new method and basis for the structural monitoring of the multi-point hoisting of a long-span converter station steel structure.
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http://dx.doi.org/10.3390/s21144737DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8309508PMC
July 2021

Noggin, an inhibitor of bone morphogenetic protein signaling, antagonizes TGF-β1 in a mouse model of osteoarthritis.

Biochem Biophys Res Commun 2021 Jul 20;570:199-205. Epub 2021 Jul 20.

Department of Orthopaedic Surgery, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, China; Guangdong Provincial Key Laboratory of Orthopedics and Traumatology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China. Electronic address:

Osteoarthritis (OA) is the most common joint disease worldwide; however, disease-modifying treatments are lacking because of the complicated pathological mechanisms. As a breakthrough, aberrant activation of transforming growth factor-β 1 (TGF-β1)in subchondral bone has been confirmed as an essential pathomechanism for OA progression, and has become a potential therapeutic target. In addition to R&D on neutralizing antibodies, small-molecule antagonists and chemical medicines, native antagonists of TGF-β1 could be exploited as another promising approach. Noggin (NOG) is an antagonist of bone morphogenetic proteins (BMPs) and was reported to effectively attenuate OA by protecting cartilage and preventing pathological subchondral bone remodeling. However, the underlying mechanisms have not been fully clarified. We first detected the distribution of NOG in knee joints of an OA mouse model, which showed upregulation at early stage of OA but downregulation later in the subchondral bone and no significant change in the articular cartilage. Furthermore, the interaction between NOG and TGF-β1 was verified, which in turn suppressed the downstream SMAD2/3 activity of TGF-β1. Moreover, the proliferation and chondrogenesis of mesenchymal stem cells (MSCs) were not significantly influenced by NOG. Taken together, the results showed that NOG antagonized TGF-β1 but did not repress MSC proliferation and chondrogenesis; thus, it seems promising for OA treatment.
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http://dx.doi.org/10.1016/j.bbrc.2021.07.044DOI Listing
July 2021

Gastrodin improves nonalcoholic fatty liver disease via activation of the AMPK signaling pathway.

Hepatology 2021 Jul 23. Epub 2021 Jul 23.

College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.

Background & Aims: Nonalcoholic steatohepatitis (NASH) is currently one of the most common causes of liver transplantation and hepatocellular carcinoma. Thus far, there is still no effective pharmacological therapy for this disease. Recently, Gastrodin has demonstrated hepatoprotective effects in a variety of liver diseases. The aim of this study is to investigate the function of Gastrodin in NASH.

Approach And Result: In our study, Gastrodin showed potent therapeutic effects on NASH both in vivo and in vitro. In high-fat diet (HFD)- or high-fat and high-cholesterol (HFHC) diet-fed mice, the liver weight, hepatic and serum triglyceride and cholesterol contents, the serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activity levels were markedly reduced by Gastrodin treatment as compared to the corresponding vehicle groups. Notably, Gastrodin showed minimal effects on the function and histological characteristics of other major organs in mice. We further examined the effects of Gastrodin on lipid accumulation in primary mouse hepatocytes and human hepatocyte cell line, and observed that Gastrodin showed a significant decrease in lipid accumulation and inflammatory response in hepatocytes under metabolic stress. Furthermore, RNA-Seq analysis systemically indicated that Gastrodin suppressed the pathway and key regulators related to lipid accumulation, inflammation and fibrosis in the pathogenesis of NASH. Mechanistically, we found that Gastrodin protected against NASH by activating the AMPK pathway, which was supported by the result that the AMPK inhibitor compound C or AMPK knockdown blocked the Gastrodin-mediated hepatoprotective effect.

Conclusion: Gastrodin attenuates steatohepatitis by activating the AMPK pathway and represents a novel therapeutic for the treatment of NASH.
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http://dx.doi.org/10.1002/hep.32068DOI Listing
July 2021

Identification of microRNA-like RNAs from Trichoderma asperellum DQ-1 during its interaction with tomato roots using bioinformatic analysis and high-throughput sequencing.

PLoS One 2021 22;16(7):e0254808. Epub 2021 Jul 22.

Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests (Hainan University), Ministry of Education, Haikou, Hainan, PR China.

MicroRNA-like small RNAs (milRNAs) and their regulatory roles in the interaction between plant and fungus have recently aroused keen interest of plant pathologists. Trichoderma spp., one of the widespread biocontrol fungi, can promote plant growth and induce plant disease resistance. To investigate milRNAs potentially involved in the interaction between Trichoderma and tomato roots, a small RNA (sRNA) library expressed during the interaction of T. asperellum DQ-1 and tomato roots was constructed and sequenced using the Illumina HiSeqTM 2500 sequencing platform. From 13,464,142 sRNA reads, we identified 21 milRNA candidates that were similar to other known microRNAs in the miRBase database and 22 novel milRNA candidates that possessed a stable microRNA precursor hairpin structure. Among them, three milRNA candidates showed different expression level in the interaction according to the result of stem-loop RT-PCR indicating that these milRNAs may play a distinct regulatory role in the interaction between Trichoderma and tomato roots. The potential transboundary milRNAs from T. asperellum and their target genes in tomato were predicted by bioinformatics analysis. The results revealed that several interesting proteins involved in plant growth and development, disease resistance, seed maturation, and osmotic stress signal transduction might be regulated by the transboundary milRNAs. To our knowledge, this is the first report of milRNAs taking part in the process of interaction of T. asperellum and tomato roots and associated with plant promotion and disease resistance. The results might be useful to unravel the mechanism of interaction between Trichoderma and tomato.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0254808PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8297844PMC
July 2021

Mutation spectrum of amyotrophic lateral sclerosis in Central South China.

Neurobiol Aging 2021 Jun 19. Epub 2021 Jun 19.

Department of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China; Laboratory of Medical Genetics, Central South University, Changsha, Hunan, People's Republic of China; Key Laboratory of Hunan Province in Neurodegenerative Disorders, Central South University, Changsha, Hunan, People's Republic of China; National Clinical Research Center for Geriatric Diseases, Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China. Electronic address:

To analyze the mutational spectrum of known ALS causative genes in China ALS patients. We comprehensively analyzed 51 ALS causative genes by combining different sequencing technologies in 753 unrelated ALS patients from Central South China. The mean age at onset (AAO) was 53.7±11.4 years. The AAO was earlier in the autosomal dominant (AD) ALS patients than in the sporadic ALS (sALS) patients. Bulbar onset was more frequent in females than in males. SOD1 was the most frequently mutated gene in the AD-ALS and the sALS patients, followed by the ATXN2 and FUS genes in the AD-ALS patients and the NEK1 and CACNA1H genes in the sALS patients. Patients with RDVs in the SOD1 or FUS genes had an earlier AAO than the mean AAO of all the patients, while the patients with RDVs in the NEK1 gene showed later onset. SOD1 gene was the most commonly mutated gene in ALS patients in China, followed by ATXN2 and NEK1. The phenotype might be determined synergistically by sex and genetic variants.
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http://dx.doi.org/10.1016/j.neurobiolaging.2021.06.008DOI Listing
June 2021

Cellular automata based framework for evaluating mitigation strategies of sponge city.

Sci Total Environ 2021 Jul 13;796:148991. Epub 2021 Jul 13.

School of Engineering and Technology, Asian Institute of Technology, Thailand.

Urban surface water flooding is increasing because of climate change and urbanization, and brings great challenges to urban sustainable development. It is, therefore, most important to develop urban flood management approaches to alleviate the consequences of floods. China is implementing a "sponge city" initiative to tackle urban surface water flooding and improve urban water management. There is, however, limited cost-effectiveness evaluation to support the choice of economically efficient mitigation strategies. To address this gap, this study developed an evaluation framework based on cellular automata and cost-benefit analysis to assess the performance of mitigation strategies in sponge city construction. This approach is demonstrated with a case study of Siergou (Dalian, China), which has a total area of 10.1 km. The mitigation measures of green roofs, permeable pavements and bio-retention were used to generate mitigation scenarios. A two-dimensional cellular automata-based model was used to simulate urban surface water flooding. The results obtained from the case study indicate that the framework can achieve cost-effective mitigation strategies for sponge city construction, which can support robust decision making. The distribution of mitigation strategies has great impacts on the effectiveness of alleviating urban flood risk. This study provides new insight into the development of cost-effective mitigation strategies for sponge city construction.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148991DOI Listing
July 2021

An Injectable Multifunctional Dual-Phase Bead-Reinforced Gelatin Matrix Permissive of Mesenchymal Stem Cell Infiltration for Musculoskeletal Soft Tissue Repair.

Adv Healthc Mater 2021 Jul 14:e2100626. Epub 2021 Jul 14.

Tissue Engineering, VA Boston Healthcare System, Boston, MA, 02130, USA.

This study develops a novel strategy for regenerative therapy of musculoskeletal soft tissue defects using a dual-phase multifunctional injectable gelatin-hydroxyphenyl propionic acid (Gtn-HPA) composite. The dual-phase gel consists of stiff, degradation-resistant, ≈2-mm diameter spherical beads made from 8 wt% Gtn-HPA in a 2 wt% Gtn-HPA matrix. The results of a 3D migration assay show that both the cell number and migration distance in the dual-phase gel system are comparable with the 2 wt% mono-phase Gtn-HPA, but notably significantly higher than for 8 wt% mono-phase Gtn-HPA (into which few cells migrated). The results also show that the dual phase gel system has degradation resistance and a prolonged growth factor release profile comparable with 8 wt% mono-phase Gtn-HPA. In addition, the compressive modulus of the 2 wt% dual-phase gel system incorporating the 8 wt% bead phase is nearly four-fold higher than the 2 wt% mono-phase gel (5.3 ± 0.4 kPa versus 1.5 ± 0.06 kPa). This novel injectable dual-phase Gtn-HPA composite thus combines the advantages of low-concentration Gtn-HPA (cell migration) with high-concentration Gtn-HPA (stiffness, degradation resistance, slower chemical release kinetics) to facilitate effective reparative/regenerative processes in musculoskeletal soft tissue.
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http://dx.doi.org/10.1002/adhm.202100626DOI Listing
July 2021

Canonical Wnt Signaling Drives Myopia Development and Can Be Pharmacologically Modulated.

Invest Ophthalmol Vis Sci 2021 Jul;62(9):21

Eye Institute and Affiliated Xiamen Eye Center of Xiamen University, Xiamen University, Xiamen, Fujian, China.

Purpose: The purpose of this study was to evaluate the role of the canonical Wnt signaling in the development of the myopia.

Methods: Plasma from adult patients with myopia, myopic animal models including the adenomatous polyposis coli (APC) gene mutation mouse model, and the form deprivation (FD) induced mouse model of myopia were used. Niclosamide, a canonical Wnt pathway inhibitor, was orally administrated in animal models. Plasma levels of DKK-1 were determined by using enzyme-linked immunosorbent assay. Refraction, vitreous chamber depth (VCD), axial length (AL), and other parameters, were measured at the end of the FD treatment. Canonical Wnt signaling changes were evaluated by Western blot analysis and immunostaining analysis.

Results: Plasma level of Wnt inhibitor DKK-1 was markedly decreased in patients with myopia. Meanwhile, the canonical Wnt pathway was progressively activated during myopia development in mice. Moreover, inhibition of canonical Wnt signaling by niclosamide in mouse models markedly reduced lens thickness (LT), VCD, and AL elongation, resulting in myopia inhibition.

Conclusions: Dysregulation of canonical Wnt signaling is a characteristic of myopia and targeting Wnt signaling pathways has potential as a therapeutic strategy for myopia.
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http://dx.doi.org/10.1167/iovs.62.9.21DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8288060PMC
July 2021

Regulating the morphology and size of homopolypeptide self-assemblies selective solvents.

Soft Matter 2021 Aug;17(30):7118-7123

School of Chemistry and Molecular Engineering, East China Normal University, No. 500 Dongchuan Road, Shanghai 200241, China.

It remains a great challenge to control the morphology and size of self-assembled homopolypeptide aggregates. In this work, rod-like micelles including spindles and cylinders were prepared by a solution self-assembly of poly(γ-benzyl-l-glutamate) (PBLG) homopolypeptides with different degrees of polymerization, in which their size was controlled precisely by tuning the ratio of water/methanol in selective cosolvents. The length of the rod-like micelles increased with an increasing amount of methanol in the selective cosolvents, which was confirmed using the combination of SEM, TEM and AFM. The self-assembly mechanism of PBLG in selective cosolvents was investigated by using complementary Fourier transform infrared (FT-IR), circular dichroism (CD) and low-field NMR analyses. It was found that the shrinkage and swelling of PBLG chains play important roles in the self-assembly process. The obtained results may provide a guideline for the study of regulating the assembled aggregate sizes.
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http://dx.doi.org/10.1039/d1sm00679gDOI Listing
August 2021

Fabrication of a novel one-step coating hyper-hydrophobic fluorine-free TiO decorated hollow composite membrane for use in longer-term VMD with enhanced flux, rejection, anti-wetting and anti-fouling performances.

Nanoscale 2021 Jul 13;13(28):12342-12355. Epub 2021 Jul 13.

State Key Laboratory of Separation Membranes and Membrane Processes, Tiangong University, Tianjin 300387, P. R. China.

Despite recent efforts, there are still significant challenges in preparing hyper-hydrophobic membranes using environmental-friendly materials and simple methods. In this work, using phase separation theory, we prepared a fluorine-free hyper-hydrophobic porous hollow composite membrane using one-step ultrasound dip-coating. Then, fluorine-free modified titanium dioxide, polydimethylsilane and polypropylene was used to construct the porous membrane with a water contact angle of 161°. The distribution of surface elements, morphology, wetting and the scale of titanium on the membranes was characterized using X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), scanning electron microscopy (SEM), atomic force microscopy (AFM), the water contact angle and acid-alkali stability, wetting resistance, and so on. The membrane was evaluated for desalination in the presence of organic-pollutants. Under longer-term vacuum membrane distillation, compared with the general polypropylene membrane, the flux of the hyper-hydrophobic membrane increased to 12.17 kg (m h), and the rejection rate reached 99.99%. These results indicated that the free-fluorine hyper-hydrophobic membrane could be used for seawater desalination. Finally, our results indicate that the hyper-hydrophobic modified membrane has good potential for use in industrial desalination.
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http://dx.doi.org/10.1039/d1nr02192cDOI Listing
July 2021

Clinicopathological and prognostic values of PD-L1 expression in oesophageal squamous cell carcinoma: a meta-analysis of 31 studies with 5368 patients.

Postgrad Med J 2021 Jul 12. Epub 2021 Jul 12.

Digestive Diseases Center, Wuxi Mingci Hospital, Wuxi, Jiangsu, People's Republic of China

Several immune checkpoint inhibitors targeting programmed death ligand 1 (PD-L1)/programmed death 1 have successfully improved the prognosis of oesophageal squamous cell carcinoma (ESCC) with approval in certain countries. However, whether the expression of PD-L1 is associated with the degree of benefit is unclear yet and a unified standard of antibody and cut-off value of PD-L1 detection is also lacking. The current meta-analysis then aimed to explore the association between PD-L1 expression and clinicopathological features as well as prognosis in ESCC.A systematic search on PubMed, Embase, Cochrane Library and Web of Science databases was performed up to 30 March 2021. The correlation between PD-L1 expression and clinicopathological features, as well as prognosis in ESCC, was estimated with the random-effects model.A total of 5368 patients from 31 retrospective studies were enrolled. The overexpression of PD-L1 was significantly associated with lymph node metastasis (OR 1.342, 95% CI 0.995 to 1.809, p=0.050) and distant metastasis (OR 1.516, 95% CI 1.001 to 2.294, p=0.050). The pooled HR showed that PD-L1 overexpression was significantly correlated with poor overall survival (OS) of patients with ESCC (HR 1.306, 95% CI 1.108 to 1.539, p<0.010) but not disease-free survival (DFS) (HR 1.180, 95% CI 0.937 to 1.487, p=0.160). Heterogeneity decreased significantly in subgroup analyses. The overexpression of PD-L1 was associated with poor DFS at the cut-off point of ≥1% (HR 1.642, 95% CI 1.367 to 1.973, p<0.010; I=0%) and worse OS at the cut-off point of ≥10% (HR 1.575, 95% CI 1.175 to 2.111, p<0.010; I=0%).The overexpression of PD-L1 was correlated with lymph node and distant metastasis as well as poor survival of ESCC.
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http://dx.doi.org/10.1136/postgradmedj-2021-140029DOI Listing
July 2021

Low urinary iodine is a protective factor of central lymph node metastasis in papillary thyroid cancer: a cross-sectional study.

World J Surg Oncol 2021 Jul 12;19(1):208. Epub 2021 Jul 12.

Department of General Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 1 Shuaifuyuan Wangfujing Dongcheng District, Beijing, 100730, China.

Background: An abrupt increase of thyroid cancer has been witnessed paralleling the supplemented iodine intake in formerly iodine-deficient countries. And increased iodine intake has been linked to the rising incidence rate of papillary thyroid cancer (PTC). However, the correlation between iodine and clinicopathological features of PTC has not been well-characterized. This study aimed to investigate the associations between iodine intake and the clinicopathological features of PTC patients.

Methods: Three hundred and fifty-nine PTC patients who received surgical treatment in Peking Union Medical College Hospital from May 2015 to November 2020 were retrospectively reviewed. The associations between urinary iodine (UI), urinary iodine/creatinine ratio (UI/U-Cr), and the clinicopathological features of PTC were analyzed. Univariate and multivariate analysis were performed to investigate the relationship between UI level and central lymph node metastasis (CLNM).

Results: There were no significant differences in UI in different groups according to the variables studied, except that patients with CLNM had higher UI level than CLNM(-) patients. No associations were found between UI/U-Cr and clinicopathological features except variant subtypes (classic/follicular). After dividing patients into high-iodine group and low-iodine group, more patients were found to have CLNM in the high-iodine group (p = 0.02). In addition, younger age, larger tumor size, and classic variant were positively correlated with CLNM (p < 0.05). Univariate analysis showed that insufficient iodine intake (≤ 99 μg/L) was associated with decreased CLNM risk in PTC. And after defining insufficient iodine intake as ≤ 109 μg/L and above requirements as ≥ 190 μg/L, multivariate analysis showed that lower iodine was associated with CLNM in total population of PTC (OR 0.53, 95% CI 0.31-0.91) and in PTC < 1 cm (papillary thyroid microcarcinoma, PTMC) (OR 0.43, 95% CI 0.21-0.87).

Conclusions: Low iodine was a protective factor for CLNM in papillary thyroid cancer, particularly in those < 1 cm. These results indicated that iodine may not only be an initiator of tumorigenesis, but also a promoter of the development of PTC.
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http://dx.doi.org/10.1186/s12957-021-02302-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8276512PMC
July 2021

Comparison of 3D and 2D characterization of spinal geometry from biplanar X-rays: a large cohort study.

Quant Imaging Med Surg 2021 Jul;11(7):3306-3313

Arts et Métiers Institute of Technology, Université Sorbonne Paris Nord, Institut de Biomécanique Humaine Georges Charpak (IBHGC), HESAM Université, Paris, France.

Background: Biplanar X-ray system providing anteroposterior and sagittal plane with an ultra-low radiation dose and in weight-bearing position is increasingly used for spine imaging. The original three-dimensional (3D) reconstruction method from biplanar X-rays has been widely used for clinical parameters, however, the main issue is that manual adjustments of the 3D model was quite time-consuming and limited to thoracolumbar spine. A quasi-automated 3D reconstruction method of the spine from cervical vertebra to pelvis was proposed, which proved fast and accurate in 57 patients with adolescent idiopathic scoliosis. The aim of this study was to compare the newly developed technique of quasi-automatic 3D measurement with classical 2D measurements in a large cohort.

Methods: A total of 494 adults with biplanar EOS X-ray scanning were included in this study and divided into health and deformity group according to the presence of spinal deformity. The proposed method of quasi-automatic 3D measurement was applied to all these subjects. The radiographic parameters included: thoracic kyphosis (TK), lumbar lordosis (LL), pelvic incidence (PI), pelvic tilt (PT), sagittal vertical axis (SVA), T1 pelvic angle (TPA) in sagittal plane, and cobb angle in coronal plane. Comparison was made between quasi-automatic and manual measurement.

Results: The mean age was 53.7±19.9 years old. In the whole population, the mean differences between the two methods were 3.9° for TK (30.5°±9.9° 26.5°±9.3°, P<0.001), -5.2° for LL (-47.5°±11.2° -42.4°±11.0°, P<0.001), 3.6° for PI (46.9°±10.3° 43.9°±10.3°, P<0.001), -0.2° for PT (11.9°±7.7° 12.0°±8.2°, P=0.328), -2.1 mm for SVA (15.7±26.2 17.8±26.3 mm, P=0.221) and -1.1° for TPA (9.0°±7.6° 10.1°±7.8°, P=0.051). The deformity group had similar mean differences with the asymptomatic group with the values ranged from -4.1° to 3.8° for sagittal parameters. The mean differences of Cobb angle were 1.9° for patients with Cobb angle <30° and 2.3° for patients with Cobb angle >30°, respectively. Correlation analysis showed r2 for all clinical parameters ranged from 0.667 to 0.923. On average, the new method takes 5 minutes to compute all the parameters for one case.

Conclusions: In conclusion, this ergonomic and efficient quasi-automatic method for full spine proved fast and accurate measurement in a large population, which showed great potential in extensive clinical application.
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http://dx.doi.org/10.21037/qims-20-861DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8250026PMC
July 2021

Evaluation of Peripheral Immune Activation in Amyotrophic Lateral Sclerosis.

Front Neurol 2021 24;12:628710. Epub 2021 Jun 24.

Department of Neurology, Xiangya Hospital, Central South University, Changsha, China.

Accumulating evidence has revealed that immunity plays an important role in amyotrophic lateral sclerosis (ALS) progression. However, the results regarding the serum levels of immunoglobulin and complement are inconsistent in patients with ALS. Although immune dysfunctions have also been reported in patients with other neurodegenerative diseases, few studies have explored whether immune dysfunction in ALS is similar to that in other neurodegenerative diseases. Therefore, we performed this study to address these gaps. In the present study, serum levels of immunoglobulin and complement were measured in 245 patients with ALS, 65 patients with multiple system atrophy (MSA), 60 patients with Parkinson's disease (PD), and 82 healthy controls (HCs). Multiple comparisons revealed that no significant differences existed between patients with ALS and other neurodegenerative diseases in immunoglobulin and complement levels. Meta-analysis based on data from our cohort and eight published articles was performed to evaluate the serum immunoglobulin and complement between patients with ALS and HCs. The pooled results showed that patients with ALS had higher C4 levels than HCs. In addition, we found that the IgG levels were lower in early-onset ALS patients than in late-onset ALS patients and HCs, and the correlations between age at onset of ALS and IgG or IgA levels were significant positive. In conclusion, our data supplement existing literature on understanding the role of peripheral immunity in ALS.
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http://dx.doi.org/10.3389/fneur.2021.628710DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8264193PMC
June 2021

Genome-wide identification of the MIOX gene family and their expression profile in cotton development and response to abiotic stress.

PLoS One 2021 9;16(7):e0254111. Epub 2021 Jul 9.

Zhengzhou University, Zhengzhou, Henan, China.

The enzyme myo-inositol oxygenase (MIOX) catalyzes the myo-inositol into glucuronic acid. In this study, 6 MIOX genes were identified from all of the three diploid cotton species (Gossypium arboretum, Gossypium herbaceum and Gossypium raimondii) and Gossypioides kirkii, 12 MIOX genes were identified from two domesticated tetraploid cottons Gossypium hirsutum, Gossypium barbadense, and 11 MIOX genes were identified from three wild tetraploid cottons Gossypium tomentosum, Gossypium mustelinum and Gossypium darwinii. The number of MIOX genes in tetraploid cotton genome is roughly twice that of diploid cotton genome. Members of MIOX family were classified into six groups based on the phylogenetic analysis. Integrated analysis of collinearity events and chromosome locations suggested that both whole genome duplication and segmental duplication events contributed to the expansion of MIOX genes during cotton evolution. The ratios of non-synonymous (Ka) and synonymous (Ks) substitution rates revealed that purifying selection was the main force driving the evolution of MIOX genes. Numerous cis-acting elements related to light responsive element, defense and stress responsive element were identified in the promoter of the MIOX genes. Expression analyses of MIOX genes based on RNA-seq data and quantitative real time PCR showed that MIOX genes within the same group shared similar expression patterns with each other. All of these results provide the foundation for further study of the biological functions of MIOX genes in cotton environmental adaptability.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0254111PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8270170PMC
July 2021

Optimizing parenteral nutrition to achieve an adequate weight gain according to the current guidelines in preterm infants with birth weight less than 1500 g: a prospective observational study.

BMC Pediatr 2021 07 7;21(1):303. Epub 2021 Jul 7.

Department of Pediatric Gastroenterology, Hepatology and Nutrition, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, 7 Wei Wu Road, Zhengzhou, 450003, Henan, China.

Aim: European Society for Clinical Nutrition and Metabolism released the guidelines on pediatric parenteral nutrition in 2018. We aimed to compare the parenteral nutrition (PN) regimen with the current guidelines, evaluate weight gain and explore the correlation of parenteral macronutrient and energy intakes with weight gain outcome in preterm infants with birth weight less than 1500 g.

Methods: A prospective observational study was conducted. Parenteral macronutrients and energy intakes were described. Weight gain during PN was assessed. Nutritional factors associated with weight gain outcome after PN were identified using a cox proportional hazards model.

Results: A total of 163 infants were included in this study, in which 41 were extremely low birth weight (ELBW) infants and 122 were very low birth weight (VLBW) infants. Average glucose, amino acid, lipid, and energy during the first postnatal week were 7.5 g/kg/d, 2.4 g/kg/d, 0.8 g/kg/d, 48 kcal/kg/d. Median maximum glucose, amino acid, lipid, and energy were 11.1 g/kg/d, 3.5 g/kg/d, 3 g/kg/d, 78 kcal/kg/d. Median days to maximum glucose, amino acid, lipid, and energy were 10, 9, 12, 11 days. The proportion of appropriate for gestational age (AGA) infants was 76.9%. The ratio of infants without poor weight gain outcome after PN was 38%. With every 0.1 g/kg/d decrease of maximum amino acid and average lipid during the first postnatal week, the probability of appropriate weight gain outcome decreased by 77.6 and 74.4% respectively. With each additional day to maximum glucose and energy, the probability of appropriate weight gain outcome decreased by 5.6 and 6.1% respectively.

Conclusions: Most preterm infants with birth weight less than 1500 g remain below the latest recommended nutrition goals. The poor weight gain outcome of these infants after PN is related to insufficient parenteral macronutrient and energy intakes. PN strategies should be improved according to the latest evidence-based recommendations.
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http://dx.doi.org/10.1186/s12887-021-02782-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8260575PMC
July 2021

LncRNA HCG11/miR-579-3p/MDM2 axis modulates malignant biological properties in pancreatic carcinoma via Notch/Hes1 signaling pathway.

Aging (Albany NY) 2021 06 21;13(12):16471-16484. Epub 2021 Jun 21.

Department of Pancreatic and Thyroid Surgery, Shengjing Hospital, China Medical University, Shenyang, China.

Background: Increasing reports have revealed that dysregulated expression of long non-coding RNAs (lncRNAs) is involved in pancreatic carcinoma progression. This study intends to explore the function and molecular mechanism of lncRNA HLA complex group 11 (HCG11) in pancreatic carcinoma.

Methods: The expression profiles of HCG11 in pancreatic carcinoma samples were detected by qPCR. Bioinformatics analysis was applied to detect the associations among HCG11/miR-579-3p/MDM2. The malignant properties of pancreatic carcinoma cells were measured by numerous biological assays. Xenograft model was exploited to detect the effect of HCG11 on tumor growth.

Results: A significant increase of HCG11 was occurred in pancreatic carcinoma samples. Knockdown of HCG11 suppressed the progression of pancreatic carcinoma cells. Bioinformatics analysis revealed that HCG11 upregulated MDM2 expression by competitively targeting miR-579-3p. The rescue assays showed that miR-579-3p reversed cell behaviors caused by HCG11, and MDM2 reversed cell properties induced by miR-579-3p. The Notch1 intracellular domain (NICD) and Hes1 protein levels were increased by overexpression of HCG11/MDM2. The tumor growth was suppressed after depletion of HCG11, followed by suppressing Ki67, PCNA and Vimentin expression, increasing TUNEL-positive cells and E-cadherin expression.

Conclusions: Our observations highlighted that HCG11 contributed to the progression of pancreatic carcinoma by promoting growth and aggressiveness, and inhibiting apoptosis via miR-579-3p/MDM2/Notch/Hes1 axis.
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http://dx.doi.org/10.18632/aging.203167DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8266358PMC
June 2021

A psychological health support scheme for medical teams in COVID-19 outbreak and its effectiveness.

Gen Psychiatr 2020 30;33(5):e100288. Epub 2020 Aug 30.

Department of Nephrology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Background: Medical staff fighting the COVID-19 pandemic are experiencing stress from high occupational risk, panic in the community and the extreme workload. Maintaining the psychological health of a medical team is essential for efficient functioning, but psychological intervention models for emergency medical teams are rare.

Aims: To design a systematic, full-coverage psychological health support scheme for medical teams serving large-scale emergent situations, and demonstrate its effectiveness in a real-world study in Leishenshan Hospital during the COVID-19 epidemic in Wuhan, China.

Methods: The scheme integrates onsite and online mental health resources and features team-based psychosocial support and evidence-based interventions. It contained five modules, including a daily measurement of mood, a daily mood broadcast that promotes positive affirmation, a daily online peer-group activity with themes based on the challenges reported by the team, Balint groups and an after-work support team. The daily mood measurement provides information to the other modules. The scheme also respects the special psychological characteristics of medical staff by promoting their strengths.

Results: The scheme economically supported a special medical team of 156 members with only one onsite psychiatrist. Our data reflected that the entire medical team maintained an overall positive outlook (7-9 out of 10 in a Daily Mood Index, DMI) for nearly 6 weeks of continuous working. Since the scheme promoted self-strengths and positive self-affirmation, the number of self-reports of life-related gains were high and played a significant effect on the DMI. Our follow-up investigations also revealed that multiple modules of the scheme received high attention and evaluation levels.

Conclusion: Our quantitative data from Leishenshan hospital, Wuhan, China, show that the programme is adequate to support the continuous high workload of medical teams. This scheme could be applied to medical teams dealing with emergent situations.
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http://dx.doi.org/10.1136/gpsych-2020-100288DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7462042PMC
August 2020

A checklist of Scoliidae (Insecta: Hymenoptera) from China.

Zootaxa 2021 May 3;4966(2):101126. Epub 2021 May 3.

State Key Lab of Rice Biology, Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou, China.

A checklist of all confirmed species of the family Scoliidae (Hymenoptera) from China is made based on the Chinese specimens in the Naturalis Biodiversity Center (RMNH, Leiden) and on additional specimens from the Chinese Academy of Insect Science (IOZ, Beijing), the Zhejiang University (ZJUH, Hangzhou), and the Sun Yat-sen University (SYSUBM, Guangzhou). The Scoliid species from China belong to one subfamily, two tribes, 11 genera and 52 species. Two species are deleted from the fauna of China: Campsomeriella ilanensis (Tsuneki, 1972) and Scolia (Discolia) quadripustulata Fabricius, 1782. One subspecies, i.e., Carinoscolia vittifrons nipponensis Uchida, 1933, status nov., is elevated to species rank. Scolia (Discolia) formosicola Betrem, 1928, status nov. and S. (D.) inouyei Okamoto, 1924 are re-instated valid species.
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http://dx.doi.org/10.11646/zootaxa.4966.2.1DOI Listing
May 2021

Targeting HSPA8 inhibits proliferation via downregulating BCR-ABL and enhances chemosensitivity in imatinib-resistant chronic myeloid leukemia cells.

Exp Cell Res 2021 Aug 19;405(2):112708. Epub 2021 Jun 19.

Department of Clinical Laboratory, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Shanghai, PR China.

The resistance to tyrosine kinase inhibitors is currently a major problem for chronic myeloid leukemia (CML) treatment and HSPA8 is highly expressed and a hallmark of poor prognosis in several human cancers. However, its role in imatinib-resistant CML (IR-CML) cells remains undetermined. Here, we determined HSPA8 was overexpressed in IR-CML cells and associated with imatinib resistance. HSPA8 ablation could downregulate BCR-ABL/STAT5 and BCR-ABL/AKT signaling pathways, dramatically induce proliferation inhibition, autophagy, G0/G1 phase cell cycle arrest but not apoptosis in IR-CML cells. Significantly, HSPA8 ablation enhanced the antitumor activity of imatinib via promoting apoptosis in vitro and vivo. These findings unraveled that HSPA8 ablation inhibits proliferation via downregulating BCR-ABL and enhances chemosensitivity of imatinib in IR-CML cells, which investigate the role and molecular mechanism of HSPA8 in IR-CML cells and suggest that HSPA8 may be a potential target for IR-CML treatment.
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http://dx.doi.org/10.1016/j.yexcr.2021.112708DOI Listing
August 2021
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