Publications by authors named "Zhen Dong"

339 Publications

Association of dietary sulfur amino acid intake with mortality from diabetes and other causes.

Eur J Nutr 2021 Jul 29. Epub 2021 Jul 29.

Department of Public Health Sciences, Penn State Cancer Institute, The Pennsylvania State University College of Medicine, 500 University Drive, Mail Code CH69, Hershey, PA, 17033, USA.

Purpose: Sulfur amino acid (SAA) consumption in Western countries is far greater than recommended levels. In preclinical studies, reduced SAA intake enhanced longevity and reduced risk for numerous chronic diseases. The current objective was to examine for associations between the intake of total SAA, including methionine (Met) and cysteine (Cys), and all-cause and disease-specific mortality US adults.

Methods: This prospective analysis included 15,083 US adult participants (mean age = 46.7 years) from the Third National Examination and Nutritional Health Survey (NHANES III, 1988-1994) with available mortality status (National Death Registry, 1988-2011). Dietary SAA intake was obtained from 24-h recall data. Associations between quintile (Q) of SAA intake (expressed as absolute intake or protein density) and mortality were assessed using Cox proportional hazard models and expressed as hazard ratio (HR).

Results: During follow-up (mean = 16.9 years), 4636 deaths occurred. After multivariable adjustment (including demographics and traditional risk factors, such as fat and other micronutrients intake), diabetes-caused mortality rates were nearly threefold higher in the highest compared to lowest SAA intake quintiles [HR total SAA 2.68 (1.46-4.90); HR methionine, 2.45 (1.37-4.38); HR cysteine, 2.91 (1.57-5.37)] (P < 0.01)]. Higher total SAA protein density was also associated with diabetes-caused mortality [HR 1.75 (1.31-2.35)]. Associations between SAA intake and all-cause mortality, and mortality caused by other major diseases were not detected.

Conclusion: Results suggest that high-SAA diets are associated with increased risk for diabetes mortality and that lowering intake towards to Recommended Dietary Allowance levels could lead to reductions in lifetime risk.
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http://dx.doi.org/10.1007/s00394-021-02641-wDOI Listing
July 2021

Identification of Early Diagnostic and Prognostic Biomarkers WGCNA in Stomach Adenocarcinoma.

Front Oncol 2021 18;11:636461. Epub 2021 Jun 18.

State Key Laboratory of Silkworm Genome Biology, Institute of Sericulture and Systems Biology, College of Sericulture, Textile and Biomass Sciences, Southwest University, Chongqing, China.

Stomach adenocarcinoma (STAD) is a leading cause of cancer deaths, and the outcome of the patients remains dismal for the lack of effective biomarkers of early detection. Recent studies have elucidated the landscape of genomic alterations of gastric cancer and reveal some biomarkers of advanced-stage gastric cancer, however, information about early-stage biomarkers is limited. Here, we adopt Weighted Gene Co-expression Network Analysis (WGCNA) to screen potential biomarkers for early-stage STAD using RNA-Seq and clinical data from TCGA database. We find six gene clusters (or modules) are significantly correlated with the stage-I STADs. Among these, five hub genes, i.e., , , , , and are identified and significantly de-regulated in the stage-I STADs compared with the normal stomach gland tissues, which suggests they can serve as potential early diagnostic biomarkers. Moreover, we show that high expression of and is associated with poor prognosis of STAD. encodes a large chondroitin sulfate proteoglycan that is the main component of the extracellular matrix, and encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor (PDGF) family. Consistently, Gene Ontology (GO) analysis of differentially expressed genes in the STADs indicates terms associated with extracellular matrix and receptor ligand activity are significantly enriched. Protein-protein network interaction analysis (PPI) and Gene Set Enrichment Analysis (GSEA) further support the core role of and in the tumorigenesis. Collectively, our study identifies the potential biomarkers for early detection and prognosis of STAD.
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http://dx.doi.org/10.3389/fonc.2021.636461DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8249817PMC
June 2021

Circular RNA circ_0084927 regulates proliferation, apoptosis, and invasion of breast cancer cells via miR-142-3p/ERC1 pathway.

Am J Transl Res 2021 15;13(5):4120-4136. Epub 2021 May 15.

Inner Mongolia Key Laboratory of Mongolian Medicine Pharmacology for Cardio-Cerebral Vascular System Tongliao, Inner Mongolia Autonomous Region, China.

Objective: We aimed to investigate the mechanism of circular RNA circ_0084927 in the progression of breast cancer (BC).

Methods: The levels of circ_0084927, miR-142-3p, and ELKS/RAB6-interacting/CAST family member-1 (ERC1) mRNA in the BC tissues and cells were detected by qRT-PCR. CCK8, colony formation, Transwell, and flow cytometry assays were performed to examine the cell proliferation, colony formation, cell invasion, and apoptosis, respectively, in the BC cells with regulated expressions of circ_0084927, miR-142-3p, and ERC1. RNase R treatment was employed to verify the circular structure of circ_0084927. Nucleocytoplasmic separation experiment, bioinformatics analysis, dual-luciferase reporter assay, and RNA immunoprecipitation were performed to investigate the ceRNA mechanism of circ_0084927.

Results: High levels of circ_0084927 and ERC1 and low levels of miR-142-3p were detected in the BC tissues and cells. Knockdown of circ_0084927 promoted apoptosis and inhibited proliferation, colony formation, and invasion of BC cells (all P<0.05), whereas overexpression of circ_0084927 in the BC cells achieved the opposite effects. miR-142-3p is the target of circ_0084927. Overexpression of miR-142-3p could inhibit BC cell proliferation, colony formation, and cell invasion and induce apoptosis of the BC cells (all P<0.05), and the effects of miR-142-3p knockout on the BC cells could be reversed by silencing circ_0084927. miR-142-3p could target ERC1. Both ERC1 silencing and circ_0084927 knockout in the BC cells could achieve the tumor-suppressing effect, and this effect could be more remarkable under simultaneous ERC1 silencing and circ_0084927 knockout (all P<0.05).

Conclusion: Circ_0084927 can promote the progression of BC by regulating the miR-142-3p/ERC1 axis.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8205726PMC
May 2021

Alterations of bacterial communities of vocal cord mucous membrane increases the risk for glottic laryngeal squamous cell carcinoma.

J Cancer 2021 13;12(13):4049-4063. Epub 2021 May 13.

Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, First Hospital of Shanxi Medical University, Taiyuan 030001, China.

Bacteria are among the important factors that play a role in the balance of human health, and their relationship with some tumors has been well established. However, the association between bacteria colonizing the vocal cords and glottic laryngeal squamous cell carcinoma (GLSCC) remains unclear. Here, we investigated whether bacterial communities of the vocal cord mucous membrane play a role in the development of GLSCC. We collected tumor tissue and normal adjacent tissue (NAT) samples from 19 GLSCC patients, and the bacterial communities were compared with control samples (control) from 21 vocal cord polyps using 16S rRNA high-throughput pyrosequencing. We detected 41 phyla, 93 classes, 188 orders, 373 families, and 829 genera in the vocal cord mucous membrane. A comparison of the bacterial communities in the NAT samples showed higher α-diversity than in the tumor samples. In the tumor samples, seven groups of bacteria, i.e., the phylum Fusobacteria, the class Fusobacteriia, the order Fusobacteriales, the family Fusobacteriaceae, and the genera , , and , were significantly enriched, as revealed by linear discriminant analysis coupled with effect size measurements (LEfSe). However, bacteria from the phylum Firmicutes were most significantly enriched in the vocal cord polyp tissues. These findings suggest alterations in the bacterial community structure of the vocal cord mucous membrane of GLSCC patients and that seven groups of bacteria are related to GLSCC, indicating that imbalances in bacterial communities increase the risk for the development of GLSCC.
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http://dx.doi.org/10.7150/jca.54221DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176248PMC
May 2021

GSDMD-Mediated Cardiomyocyte Pyroptosis Promotes Myocardial I/R Injury.

Circ Res 2021 Jul 21;129(3):383-396. Epub 2021 May 21.

Department of Cardiology, Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai, China (H.S., Y.G., Z.D., J.Y., X.L., S.Z., L.M., F.Z., K.H., A.S., J.G.).

[Figure: see text].
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http://dx.doi.org/10.1161/CIRCRESAHA.120.318629DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8291144PMC
July 2021

Efficient separation and recovery of Re(VII) from Re/U bearing acidic solutions using aminotriazole modified cellulose microsphere adsorbents.

Environ Sci Pollut Res Int 2021 May 18. Epub 2021 May 18.

State Key Laboratory of Advanced Electromagnetic Engineering and Technology, School of Electrical and Electronic Engineering, Huazhong University of Science and Technology, Wuhan, 430074, China.

In this work, aminotriazole-modified microcrystalline cellulose microsphere (3-ATAR) containing an abundant nitrogen content as promising adsorbent was prepared via a radiation grafting method for the selective recovery ReO in the presence of UO in acidic solution. A series of batch and column adsorption experiments including monocomponent and binary systems were designed for evaluating the adsorption and separation performance of Re(VII) onto 3-ATAR. The 3-ATAR exhibited a good adsorption capacity (max 146.4 mg·g) of Re(VII) and a rapid adsorption rate, with equilibrium time of 45 min. In binary solution, the high selectivity coefficients (β) indicated that 3-ATAR could separate and recover Re(VII) from U(VI) and other metal ions (Cu(II), Cr(III), Ni(II), Zn(II)). In particular, it was found that the adsorption of Re was almost unaffected in U/Re-bearing solutions no matter how much the U(VI) was changed. In the column experiment, when the concentration of U(VI) was 40 times higher than that of Re(VII), 3-ATAR manifested high Re(VII) selectivity over U(VI) from a synthetic uranium ore leachate. This work demonstrated that 3-ATAR could provide an efficient, selectively, sustainable, and industrially feasible way for Re(VII) to be recovered from uranium ore leachate and other prospective sources.Graphical abstract.
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http://dx.doi.org/10.1007/s11356-021-14356-wDOI Listing
May 2021

Deficiency of mitochondrial aldehyde dehydrogenase increases type 2 diabetes risk in males via autophagy dysregulation.

Chin Med J (Engl) 2021 Apr 1. Epub 2021 Apr 1.

Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China Xiangya Hospital, Central South University, Changsha, Hunan 410008, China Huashan Hospital, Fudan University, Shanghai 200040, China Institute of Biomedical Science, Fudan University, Shanghai 200032, China.

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http://dx.doi.org/10.1097/CM9.0000000000001408DOI Listing
April 2021

Bioactive Molecular Discovery Using Deer Antlers as a Model of Mammalian Regeneration.

J Proteome Res 2021 05 26;20(5):2167-2181. Epub 2021 Mar 26.

Sir John Walsh Research Institute, Faculty of Dentistry, University of Otago, Dunedin 9016, New Zealand.

The ability to activate and regulate stem cells during wound healing and tissue regeneration is a promising field that is resulting in innovative approaches in the field of regenerative medicine. The regenerative capacity of invertebrates has been well documented; however, in mammals, stem cells that drive organ regeneration are rare. Deer antlers are the only known mammalian structure that can annually regenerate to produce a tissue containing dermis, blood vessels, nerves, cartilage, and bone. The neural crest derived stem cells that drive this process result in antlers growing at up to 2 cm/day. Deer antlers thus provide superior attributes compared to lower-order animal models, when investigating the regulation of stem cell-based regeneration. Antler stem cells can therefore be used as a model to investigate the bioactive molecules, biological processes, and pathways involved in the maintenance of a stem cell niche, and their activation and differentiation during organ formation. This review examines stem cell-based regeneration with a focus on deer antlers, a neural crest stem cell-based mammalian regenerative structure. It then discusses the omics technical platforms highlighting the proteomics approaches used for investigating the molecular mechanisms underlying stem cell regulation in antler tissues.
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http://dx.doi.org/10.1021/acs.jproteome.1c00003DOI Listing
May 2021

A prediction model of delayed graft function in deceased donor for renal transplant: a multi-center study from China.

Ren Fail 2021 Dec;43(1):520-529

Tianjin First Central Hospital, Tianjin, China.

Background: Kidneys obtained from deceased donors increase the incidence of delayed graft function (DGF) after renal transplantation. Here we investigated the influence of the risk factors of donors with DGF, and developed a donor risk scoring system for DGF prediction.

Methods: This retrospective study was conducted in 1807 deceased kidney donors and 3599 recipients who received donor kidneys transplants in 29 centers in China. We quantified DGF associations with donor clinical characteristics. A donor risk scoring system was developed and validated using an independent sample set.

Results: The incidence of DGF from donors was 19.0%. Six of the donor characteristics analyzed, i.e., age, cause of death, history of hypertension, terminal serum creatinine, persistence of hypotension, and cardiopulmonary resuscitation (CPR) time were risk factors for DGF. A 49-point scoring system of donor risk was established for DGF prediction and exhibited a superior degree of discrimination. External validation of DGF prediction revealed area under the receiver-operating characteristic (AUC) curves of 0.7552.

Conclusions: Our study determined the deceased donor risk factors related to DGF after renal transplantation pertinent to the Chinese cohort. The scoring system developed here had superior diagnostic significance and consistency and can be used by clinicians to make evidence-based decisions on the quality of kidneys from deceased donors and guide renal transplantation therapy.
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http://dx.doi.org/10.1080/0886022X.2021.1895838DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7971200PMC
December 2021

Transient impulses enhancement based on adaptive multi-scale improved differential filter and its application in rotating machines fault diagnosis.

ISA Trans 2021 Mar 6. Epub 2021 Mar 6.

Centre for Efficiency and Performance Engineering, University of Huddersfield, Huddersfield, HD1 3DH, UK.

Transient impulses caused by local defects are critical for the fault detection of rotating machines. However, they are extremely weak and overwhelmed in the strong noise and harmonic components, making the transient features are very difficult to be extracted. This paper proposes an adaptive multi-scale improved differential filter (AMIDIF) to enhance the identification of transient impulses for rotating machine fault diagnosis. In this scheme, firstly, the AMIDIF is performed to decompose the measured signal of rotating machine into a series of multi-scale improved differential filter (MIDIF) filtered signals. Subsequently, in view of the MIDIF filtered signals exhibit varying extents of validity in revealing fault features, a weighted reconstruction method using correlation analysis is proposed in which the weighted coefficients are counted and distributed to the corresponding MIDIF filtered signals to highlight the effective MIDIF filtered signals and weaken the invalid ones. Finally, the transient impulse components of rotating machinery are obtained by multiplying the weighted coefficients and the MIDIF filtered signals under different scales. Furthermore, the fault types of rotating machines are inferred from the fault defect frequencies in the envelope spectrum of the transient impulses. Simulation analysis and experimental studies are implemented to verify the performance of the AMIDIF compared with the state-of-the-art methods including spectral kurtosis (SK), multi-scale average combination different morphological filter (ACDIF) and multi-scale morphology gradient product operation (MGPO). The results prove that the AMIDIF has excellent performance in extracting transient features for rotating machines fault diagnosis.
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http://dx.doi.org/10.1016/j.isatra.2021.03.005DOI Listing
March 2021

IL-32 Promotes the Radiosensitivity of Esophageal Squamous Cell Carcinoma Cell through STAT3 Pathway.

Biomed Res Int 2021 17;2021:6653747. Epub 2021 Feb 17.

Department of Radiotherapy, Lianyungang Municipal Oriental Hospital Affiliated to Bengbu Medical Collage, Lianyungang, 222042 Jiangsu, China.

Objective: This study is set out to determine the relationship between IL-32 and radiosensitivity of esophageal squamous cell carcinoma (ESCC).

Methods: Western blot was adopted for measuring IL-32 expression in Eca-109 and TE-10 cells. Eca-109 and TE-10 cells with interference or overexpression of IL-32 were treated with the presence or absence of X-ray irradiation. Then, the use of CCK8 assay was to detect proliferation ability, and effects of IL-32 expression on radiosensitivity of ESCC were tested by colony formation assay. The cell apoptosis was detected using flow cytometry. STAT3 and p-STAT expression, and apoptotic protein Bax were detected by western blot.

Results: Colony formation assay and CCK8 assay showed that compared with the NC group without treatment, the growth of the ESCC cells, that is Eca-109 and TE-10, was significantly inhibited in the OE+IR group with highly expressed IL-32 and irradiation. In flow cytometry analysis, in Eca-109 and TE-10 cells, highly expressed IL-32 combined with irradiation significantly increased apoptosis compared with the control group. Highly expressed IL-32 has a synergistic effect with irradiation, inhibiting STAT3 and p-STAT3 expression and increasing apoptotic protein Bax expression.

Conclusion: IL-32 can improve the radiosensitivity of ESCC cells by inhibiting the STAT3 pathway. Therefore, IL-32 can be used as a new therapeutic target to provide a new attempt for radiotherapy of ESCC.
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http://dx.doi.org/10.1155/2021/6653747DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7904356PMC
May 2021

Silenced long non-coding RNA activated by DNA damage elevates microRNA-495-3p to suppress atherosclerotic plaque formation via reducing Krüppel-like factor 5.

Exp Cell Res 2021 Apr 23;401(2):112519. Epub 2021 Feb 23.

Medicinal Chemistry and Pharmacology Institute, Inner Mongolia University for Nationalities, Tongliao, Inner Mongolia Autonomous Region, 028002, PR China; Inner Mongolia Key Laboratory of Mongolian Medicine Pharmacology for Cardio-Cerebral Vascular System, Tongliao, Inner Mongolia, PR China. Electronic address:

Objective: Atherosclerosis (AS) is an inflammatory disease and the formation of atherosclerotic plaque plays a critical role in AS progression. We aimed to investigate the effect of long non-coding RNA (lncRNA) activated by DNA damage (NORAD)/microRNA-495-3p (miR-495-3p)/Krüppel-like factor 5 (KLF5) axis on atherosclerotic plaque formation.

Methods: The ApoE mice were fed a high-fat diet to construct AS mouse models and the modeled mice were treated with altered NORAD, miR-495-3p or KLF5. NORAD, miR-495-3p and KLF5 expression in mouse aorta tissues were evaluated, and the levels of inflammatory factors, oxidative stress factors, endothelial function indices and blood lipid in mice were all determined. The atherosclerotic plaque area, lipid deposition area, collagen fibers and CD68 expression in mouse aorta tissues were assessed. The regulatory relation between NORAD and miR-495-3p, and the target relation between miR-495-3p and KLF5 were confirmed.

Results: NORAD and KLF5 were increased whereas miR-495-3p was decreased in atherosclerotic mouse aortas. Inhibited NORAD or elevated miR-495-3p suppressed inflammation, oxidative stress, endothelial dysfunction, blood lipid level, atherosclerotic plaque area, collagen fibers and CD68 expression in atherosclerotic mouse aortas. Effects of elevated miR-495-3p on atherosclerotic mice could be reversed by up-regulation of KLF5. NORAD served as a sponge of miR-495-3p and miR-495-3p directly targeted KLF5.

Conclusion: Silenced NORAD elevated miR-495-3p to suppress atherosclerotic plaque formation via reducing KLF5. Findings in our research may be helpful for exploring molecular mechanisms of AS.
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http://dx.doi.org/10.1016/j.yexcr.2021.112519DOI Listing
April 2021

Detection of Infectious Agents Causing Neonatal Calf Diarrhea on Two Large Dairy Farms in Yangxin County, Shandong Province, China.

Front Vet Sci 2020 5;7:589126. Epub 2021 Feb 5.

Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences (CAAS), Lanzhou, China.

Neonatal calf diarrhea (NCD) is one of the most serious health challenges facing the livestock industry and has caused substantial economic losses due to increased morbidity and mortality rates. The present study investigated the main infectious pathogens causing NCD among cattle in Yangxin County, China. Sixty-nine fecal samples were collected from diarrheic newborn cattle and tested for infectious agents, including bovine rotavirus, bovine coronavirus, K99, , and , that cause NCD, as determined by rapid kit analysis and polymerase chain reaction (PCR) amplification. The PCR results showed that the percentages of samples that were positive for , bovine rotavirus A, bovine coronavirus, and were 44.93, 36.23, 17.39, and 13.04%, respectively. The rapid kit analysis results showed that the prevalence of , rotavirus, coronavirus, and was 52.17, 31.88, 28.98, and 18.84%, respectively. No K99 was detected by either method. The total positivity of the samples, as determined by PCR and rapid kit analysis, was 80.00 and 81.16%, respectively. No significant difference between the two methods was observed. The results of this study may help to establish a foundation for future research investigating the epidemiology of NCD in cattle and may facilitate the implementation of measures to control NCD transmission to cattle in Yangxin County, Shandong Province, China.
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http://dx.doi.org/10.3389/fvets.2020.589126DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7892430PMC
February 2021

Aminotriazole isomers modified cellulose microspheres for selective adsorption of U(VI): Performance and mechanism investigation.

Carbohydr Polym 2021 Apr 16;257:117666. Epub 2021 Jan 16.

State Key Laboratory of Advanced Electromagnetic Engineering and Technology, School of Electrical and Electronic Engineering, Huazhong University of Science and Technology, Wuhan 430074, China. Electronic address:

Aminotriazole isomers with high nitrogen content show potential affinity for U(VI). Combining the advantages of both aminotriazole and cellulose, two n-aminotriazole isomers modified cellulose microspheres (n-ATARs, n = 3, 4) were newly prepared as adsorbents via radiation technology. Batch adsorption experiments showed that n-ATARs possessed good adsorption capacity of U(VI) in the order of 3-ATAR > 4-ATAR, and the adsorption process followed the Langmuir-Freundlich isotherm model and the pseudo-second-order kinetic model. Both n-ATARs exhibited good selectivity and reusability. Besides, 3-ATAR and 4-ATAR were more suitable for the extraction of trace amount of U(VI) from the contaminated groundwater and the simulated seawater, respectively. The effect of amino position on triazole ring on adsorption behavior of n-ATAR was studied and explained by DFT calculation. Finally, the adsorption mechanism of U(VI) onto n-ATARs was revealed to be inner-sphere complexation via different 1:1 or 1:2 coordination models by FTIR, MS, XPS analysis and DFT calculation.
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http://dx.doi.org/10.1016/j.carbpol.2021.117666DOI Listing
April 2021

Alda-1 treatment promotes the therapeutic effect of mitochondrial transplantation for myocardial ischemia-reperfusion injury.

Bioact Mater 2021 Jul 8;6(7):2058-2069. Epub 2021 Jan 8.

Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.

Mitochondrial damage is a critical driver in myocardial ischemia-reperfusion (I/R) injury and can be alleviated via the mitochondrial transplantation. The efficiency of mitochondrial transplantation is determined by mitochondrial vitality. Because aldehyde dehydrogenase 2 (ALDH2) has a key role in regulating mitochondrial homeostasis, we aimed to investigate its potential therapeutic effects on mitochondrial transplantation via the use of ALDH2 activator, Alda-1. Our present study demonstrated that time-dependent internalization of exogenous mitochondria by cardiomyocytes along with ATP production were significantly increased in response to mitochondrial transplantation. Furthermore, Alda-1 treatment remarkably promoted the oxygen consumption rate and baseline mechanical function of cardiomyocytes caused by mitochondrial transplantation. Mitochondrial transplantation inhibited cardiomyocyte apoptosis induced by the hypoxia-reoxygenation exposure, independent of Alda-1 treatment. However, promotion of the mechanical function of cardiomyocytes exposed to hypoxia-reoxygenation treatment was only observed after mitochondrial Alda-1 treatment and transplantation. By using a myocardial I/R mouse model, our results revealed that transplantation of Alda-1-treated mitochondria into mouse myocardial tissues limited the infarction size after I/R injury, which was at least in part due to increased mitochondrial potential-mediated fusion. In conclusion, ALDH2 activation in mitochondrial transplantation shows great potential for the treatment of myocardial I/R injury.
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http://dx.doi.org/10.1016/j.bioactmat.2020.12.024DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7809100PMC
July 2021

Deletion of MicroRNA-144/451 Cluster Aggravated Brain Injury in Intracerebral Hemorrhage Mice by Targeting 14-3-3ζ.

Front Neurol 2020 12;11:551411. Epub 2021 Jan 12.

Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

This study aims at evaluating the importance and its underlying mechanism of the cluster of microRNA-144/451 (miR-144/451) in the models with intracerebral hemorrhage (ICH). A model of collagenase-induced mice with ICH and a model of mice with simple miR-144/451 gene knockout (KO) were used in this study. Neurodeficits and the water content of the brain of the mice in each group were detected 3 days after collagenase injection. The secretion of proinflammatory cytokines, such as tumor necrosis factor α (TNF-α) and interleukin 1β (IL-1β), as well as certain biomarkers of oxidative stress, was determined in this study. The results revealed that the expression of miR-451 significantly decreased in the mice with ICH, whereas miR-144 showed no significant changes. KO of the cluster of miR-144/451 exacerbated the neurological deficits and brain edema in the mice with ICH. Further analyses demonstrated that the KO of the cluster of miR-144/451 significantly promoted the secretion of TNF-α and IL-1β and the oxidative stress in the perihematomal region of the mice with ICH. In addition, the miR-144/451's depletion inhibited the regulatory axis' activities of miR-451-14-3-3ζ-FoxO3 in the mice with ICH. In conclusion, these data demonstrated that miR-144/451 might protect the mice with ICH against neuroinflammation and oxidative stress by targeting the pathway of miR-451-14-3-3ζ-FoxO3.
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http://dx.doi.org/10.3389/fneur.2020.551411DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7835478PMC
January 2021

Immunodiagnosis and Immunotherapeutics Based on Human Papillomavirus for HPV-Induced Cancers.

Front Immunol 2020 8;11:586796. Epub 2021 Jan 8.

State Key Laboratory of Silkworm Genome Biology, Institute of Sericulture and Systems Biology, College of Sericulture & Textile & Biomass Science, Southwest University, Chongqing, China.

Infection with human papillomavirus (HPV) is one of the main causes of malignant neoplasms, especially cervical, anogenital, and oropharyngeal cancers. Although we have developed preventive vaccines that can protect from HPV infection, there are still many new cases of HPV-related cancers worldwide. Early diagnosis and therapy are therefore important for the treatment of these diseases. As HPVs are the major contributors to these cancers, it is reasonable to develop reagents, kits, or devices to detect and eliminate HPVs for early diagnosis and therapeutics. Immunological methods are precise strategies that are promising for the accurate detection and blockade of HPVs. During the last decades, the mechanism of how HPVs induce neoplasms has been extensively elucidated, and several oncogenic HPV early proteins, including E5, E6, and E7, have been shown to be positively related to the oncogenesis and malignancy of HPV-induced cancers. These oncoproteins are promising biomarkers for diagnosis and as targets for the therapeutics of HPV-related cancers. Importantly, many specific monoclonal antibodies (mAbs), or newly designed antibody mimics, as well as new immunological kits, devices, and reagents have been developed for both the immunodiagnosis and immunotherapeutics of HPV-induced cancers. In the current review, we summarize the research progress in the immunodiagnosis and immunotherapeutics based on HPV for HPV-induced cancers. In particular, we depict the most promising serological methods for the detection of HPV infection and several therapeutical immunotherapeutics based on HPV, using immunological tools, including native mAbs, radio-labelled mAbs, affitoxins (affibody-linked toxins), intracellular single-chain antibodies (scFvs), nanobodies, therapeutical vaccines, and T-cell-based therapies. Our review aims to provide new clues for researchers to develop novel strategies and methods for the diagnosis and treatment of HPV-induced tumors.
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http://dx.doi.org/10.3389/fimmu.2020.586796DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7820759PMC
June 2021

A comparative study of immobilizing ammonium molybdophosphate onto cellulose microsphere by radiation post-grafting and hybrid grafting for cesium removal.

Environ Pollut 2021 Jan 5;273:116432. Epub 2021 Jan 5.

State Key Laboratory of Advanced Electromagnetic Engineering and Technology, School of Electric and Electronic Engineering, Huazhong University of Science and Technology, 430074, Wuhan, China. Electronic address:

Ammonium molybdophosphate (AMP) exhibits high selectivity towards Cs but it cannot be directly applied in column packing, so it is necessary to prepare AMP-based adsorbents into an available form to improve its practicality. This work provided two AMP immobilized cellulose microspheres ([email protected] and MCC-g-AMP) as adsorbents for Cs removal by radiation grafting technique. MCC-g-AMP was prepared by radiation graft polymerization of GMA on microcrystalline cellulose microspheres (MCC) followed by reaction with AMP suspension, and [email protected] was synthesized by radiation hybrid grafting of AMP and GMA onto MCC through one step. The different structures and morphologies of two adsorbents were characterized by FTIR and SEM. The adsorption properties of two adsorbents against Cs were investigated and compared in batch and column experiments under different conditions. Both adsorbents were better obeyed pseudo-second-order kinetic model and Langmuir model. MCC-g-AMP presented faster adsorption kinetic and more stable structure, whereas [email protected] presented more facile synthesis and higher adsorption capacity. [email protected] was pH independent in the range of pH 1-12 but MCC-g-AMP was sensitive to pH for Cs removal. The saturated column adsorption capacities of [email protected] and MCC-g-AMP were 5.4 g-Cs/L-ad and 0.75 g-Cs/L-ad in column adsorption experiments at SV 10 h. Both adsorbents exhibited very high radiation stability and can maintain an adsorption capacity of >85% even after 1000 kGy γ-irradiation. On the basis, two AMP-loaded adsorbents possessed promising application in removal of Cs from actual contaminated groundwater. These findings provided two efficient adsorbents for treatment of Cs in radioactive waste disposal.
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http://dx.doi.org/10.1016/j.envpol.2021.116432DOI Listing
January 2021

Structurally modified glycyrrhetinic acid derivatives as anti-inflammatory agents.

Bioorg Chem 2021 02 29;107:104598. Epub 2020 Dec 29.

College of Pharmacy, Yanbian University, Yanji City, Jilin, China. Electronic address:

With the aim of finding new anti-inflammatory drugs, a series of new Glycyrrhetinic acid derivatives (1-34) have been designed and synthesized by structural modification, and their anti-inflammatory activities in vitro have been evaluated. The anti-inflammatory activities assay demonstrated that compound 5b suppressed the expression of pro-inflammatory cytokines including IL-6, TNF-α and NO, it also suppressed the expression of iNOS and COX-2 in LPS-induced RAW264.7 cells in a dose-dependent manner. Additionally, western blot results indicated that the suppressing effect of compound 5b on pro-inflammatory cytokines were correlated with the suppression of NF-κB and MAPK signaling pathways. The results attained in this study indicated that compound 5b had the potential to be developed into an anti-inflammation agent and it may be applied to the prevention and treatment of inflammatory diseases.
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http://dx.doi.org/10.1016/j.bioorg.2020.104598DOI Listing
February 2021

Recent advances in microencapsulation of drugs for veterinary applications.

J Vet Pharmacol Ther 2021 May 13;44(3):298-312. Epub 2021 Jan 13.

Key Laboratory of Veterinary Pharmaceutical Development, Ministry of Agriculture, Lanzhou, China.

Microencapsulation is a process where very minute droplets or particles of solid or liquid or gas are trapped with a polymer to isolate the internal core material from external environmental hazards. Microencapsulation is applied mostly for flavor masking, fortification, and sustained and control release. It improves palatability, absorption, and bioavailability of drugs with good conformity. Microencapsulation has been widely studied in numerous drug delivery systems for human health. The application of microcapsules in the veterinary pharmaceutical sciences is increasing day by day. The treatment systems for humans and animals are likely to be similar, but more complex in the veterinary field due to the diversity of the species, breeds, body size, biotransformation rate, and other factors associated with animal physiology. Commercially viable, economically profitable, and therapeutically effective microencapsulated vaccine, anthelmintic, antibacterial, and other therapeutics have a great demand for livestock and poultry production. Nowadays, researchers emphasize the controlled and sustained-release dosage form of drugs in the veterinary field. This paper has highlighted the microencapsulation materials, preparation techniques, characteristics, roles, and the application of microcapsules in veterinary medicine.
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http://dx.doi.org/10.1111/jvp.12946DOI Listing
May 2021

Radiation synthesis of ionic liquid-functionalized silica-based adsorbents: a preliminary investigation on its application for removal of ReO as an analog for TcO.

Environ Sci Pollut Res Int 2021 Apr 5;28(14):17752-17762. Epub 2021 Jan 5.

State Key Laboratory of Advanced Electromagnetic Engineering and Technology, School of Electrical and Electronic Engineering, Huazhong University of Science and Technology, Wuhan, 430074, China.

The decontamination of radioactive TcO from nuclear wastes is becoming increasingly crucial for spent nuclear fuel reprocessing and environmental remediation. In this work, a series of ionic liquid-immobilized silica-based adsorbents (SVIL-Cn, n = 1, 4, 8) were newly synthesized using the radiation-induced grafting of 4-vinylbenzyl chloride onto silanized silica and subsequent functionalization with 1-methylimidazole, 1-butylimidazole, or 1-octylimidazole. The synthesis conditions such as the solvent, absorbed dose, and monomer concentration were investigated in detail, and the resulting adsorbents were characterized by elemental analysis, FT-IR, SEM, and TGA. In batch experiments, the adsorbents exhibited a high ReO (a nonradioactive surrogate of TcO) removal efficiency over a wide pH range (3 ~ 8), and SVIL-C1 showed a maximum adsorption capacity of 70.62 mg g towards ReO. In addition, their adsorption performance barely changed after 800 kGy radiation. The column experiments for treating simulated radioactive wastewater showed that the SVIL-Cn adsorbents could selectively separate TcO/ReO from a variety of fission products, and they could be recycled four times with negligible capacity loss. Lastly, XPS and FT-IR analysis confirmed that the adsorption proceeded via an ion-exchange mechanism. The results showed that these adsorbents are suitable for the efficient removal of TcO/ReO from radioactive wastewater with complex compositions.
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http://dx.doi.org/10.1007/s11356-020-12078-zDOI Listing
April 2021

14-Day Repeated Intraperitoneal Toxicity Test of Ivermectin Microemulsion Injection in Wistar Rats.

Front Vet Sci 2020 16;7:598313. Epub 2020 Dec 16.

Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences (CAAS), Lanzhou, China.

To evaluate the safety of ivermectin microemulsion injection, 100 Wistar rats were injected intraperitoneally at 0.38 g/kg, 0.19 g/kg, and 0.1 g/kg for 14 days. The 14-day repeated toxicity test of ivermectin microemulsion injection was systematically evaluated by clinical observation, organ coefficient, hematological examination, clinical chemistry examination, and histopathological examination. The results showed that no rats died during the test. At the initial stage of treatment, the rats in the high dose group had mild clinical reaction, which disappeared after 4 days. Clinical chemistry showed that the high dose of ivermectin microemulsion could cause significant changes in ALT and LDH parameters in male rats; high and medium doses could increase the liver coefficients of male and female rats. The toxic target organ may be the liver as indicated by histopathological findings. No significant toxic injury was found in the heart, liver, spleen, lung, kidney, brain, ovary, and testes of all groups of rats. No drug-related toxic effects were found at low doses, and thus the NOVEL of ivermectin microemulsion injection was 0.19 g/kg.
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http://dx.doi.org/10.3389/fvets.2020.598313DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7772420PMC
December 2020

Tubeimoside I Inhibits Cell Proliferation and Induces a Partly Disrupted and Cytoprotective Autophagy Through Rapidly Hyperactivation of MEK1/2-ERK1/2 Cascade via Promoting PTP1B in Melanoma.

Front Cell Dev Biol 2020 17;8:607757. Epub 2020 Dec 17.

State Key Laboratory of Silkworm Genome Biology, Institute of Sericulture and Systems Biology, College of Sericulture and Textile and Biomass, Chongqing, China.

Tubeimoside I (TBMS1), also referred to as tubeimoside A, is a natural compound extracted from the plant Tu Bei Mu (), which is a traditional Chinese herb used to treat multiple diseases for more than 1,000 years. Studies in recent years reported its anti-tumor activity in several cancers. However, whether it is effective in melanoma remains unknown. In the current study, we discovered that TBMS1 treatment inhibited melanoma cell proliferation and tumorigenecity . Besides, we also observed that TBMS1 treatment induced a partly disrupted autophagy, which still remained a protective role, disruption of which by chloroquine (CQ) or 3-methyladenine (3-MA) enhanced TBMS1-induced cell proliferation inhibition. CQ combined with TBMS1 even induced cellular apoptosis. BRAF(V600E) mutation and its continuously activated downstream MEK1/2-ERK1/2 cascade are found in 50% of melanomas and are important for malanomagenesis. However, hyperactivating MEK1/2-ERK1/2 cascade can also inhibit tumor growth. Intriguingly, we observed that TBMS1 rapidly hyperactivated MEK1/2-ERK1/2, inhibition of which by its inhibitor SL-327 rescued the anti-cancerous effects of TBMS1. Besides, the targets of TBMS1 were predicted by the ZINC Database based on its structure. It is revealed that protein-tyrosine phosphatase 1B (PTP1B) might be one of the targets of TBMS1. Inhibition of PTP1B by its selective inhibitor TCS401 or shRNA rescued the anti-cancerous effects of TBMS1 in melanoma cells. These results indicated that TBMS1 might activate PTP1B, which further hyperactivates MEK1/2-ERK1/2 cascade, thereby inhibiting cell proliferation in melanoma. Our results provided the potentiality of TBMS1 as a drug candidate for melanoma therapy and confirmed that rapidly hyperactivating an oncogenic signaling pathway may also be a promising strategy for cancer treatment.
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http://dx.doi.org/10.3389/fcell.2020.607757DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7773826PMC
December 2020

Bruceine D inhibits Cell Proliferation Through Downregulating LINC01667/MicroRNA-138-5p/Cyclin E1 Axis in Gastric Cancer.

Front Pharmacol 2020 24;11:584960. Epub 2020 Nov 24.

State Key Laboratory of Silkworm Genome Biology, Institute of Sericulture and Systems Biology, College of Sericulture and Textile and Biomass Science, Southwest University, Chongqing, China.

Gastric cancer is one of the most common malignant tumors. Bruceine D (BD) is one of the extracts of . In recent years, it has been reported that BD has anti-tumor activity in some human cancers through different mechanisms. Here, this study try to explore the effect of BD on gastric cancer and its regulatory mechanism. Cell proliferation ability was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assays, 5-bromo-2-deoxyuridine (BrdU) staining and soft agar colony formation assay, respectively. The tumor xenograft model was used to verify the effect of BD on the tumorigenicity of gastric cancer cells . Flow cytometry analysis and Western blot assay were performed to detect cell cycle and apoptosis. Gastric cancer cells were analyzed by transcriptome sequencing. The interaction between LINC01667, microRNA-138-5p (miR-138-5p) and Cyclin E1 was verified by dual luciferase experiment and RT-PCR assays. We found that BD significantly inhibited cell proliferation and induced cell cycle arrest at S phase in gastric cancer cells. Transcriptome analysis found that the expression of a long non-coding RNA, LINC01667, were significantly down-regulated after BD treatment. Mechanically, it was discovered that LINC01667 upregulated the expression of Cyclin E1 by sponging miR-138-5p. Furthermore, BD enhanced the chemosensitivity of gastric cancer cells to doxorubicin, a clinically used anti-cancer agent. BD inhibit the growth of gastric cancer cells by downregulating the LINC01667/miR-138-5p/Cyclin E1 axis. In addition, BD enhances the chemosensitivity of gastric cancer cells to doxorubicin. This study indicates that BD may be used as a candidate drug for the treatment of patients with gastric cancer.
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http://dx.doi.org/10.3389/fphar.2020.584960DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7774499PMC
November 2020

Enhancement of Interface between Lignocellulosic Fibers and Polypropylene Matrix via the Structure Alteration of Lignin at Elevated Temperatures.

Materials (Basel) 2020 Nov 28;13(23). Epub 2020 Nov 28.

College of Textiles & Apparel, Nantong University, Nantong 226019, China.

This paper investigated the feasibility of enhancing the interface between lignocellulosic fibers and a polypropylene matrix via structure alteration of lignin at elevated temperatures. Alkali treatment can remove gum substances from lignocellulose fibers effectively at elevated temperatures but easily causes damages to fiber strength. In previous studies on directional delignification of lignocellulosic fibers, loss of fiber strength is avoided but condensation and degradation of lignin are accelerated. So far, few reports have been available on the effect of lignin structures on the interface between fibers and a matrix. In this study, jute fibers with different lignin structures are produced at 100 and 130 °C for reinforcing a polypropylene matrix. The interface between the fibers and matrix is analyzed. The result shows that decrease in aliphatic hydroxyl concentration by 9.5% at 130 °C from 3 to 5 h contributes to a 14.2% decrease in the surface energy of jute fibers. Meanwhile, the polydispersity index of lignin decreases from 1.21 to 1.15. Centralized distribution of lignin molecule-weight and reduction in fiber surface energy improves the interface between the fibers and matrix, which manifests as a 30.8% increase in the impact strength of the composites. Similar improvement is not observed in the composites reinforced with jute fibers at 100 °C, due to the absence of lignin-structure changes. This paper provides a new strategy to improve the interface between lignocellulose fibers and a hydrophobic matrix.
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http://dx.doi.org/10.3390/ma13235428DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7730693PMC
November 2020

Alternation of EEG Characteristics During Transcutaneous Acupoint Electrical Stimulation-Induced Sedation.

Clin EEG Neurosci 2020 Dec 1:1550059420976303. Epub 2020 Dec 1.

Department of Anesthesiology, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

Recent studies have shown that applying acupuncture during general anesthesia can reduce the dosage of anesthetics. Hence, it is speculated that acupuncture may have a sedative effect. However, existing studies employed acupuncture in combination with anesthetics, which makes determine acupuncture's role in producing sedation difficult. In this work, we investigated the sedative effect of acupuncture by using transcutaneous acupoint electrical stimulation (TAES) at bilateral Zusanli (ST36), Shenmen (HT7) and Sanyinjiao (SP6). Using a cross-over design, 2 separate sessions, that are, the resting and TAES sessions, were conducted for each subject. The sedative effect was quantified by using the bispectral index (BIS). The difference in brain activities between resting and TAES sessions was investigated by analyzing the simultaneously recorded EEG signals. Our results showed that a statistically significant difference in BIS values existed between resting and TAES sessions, which suggested that TAES alone was capable of inducing observable sedation. Using power spectrum analysis, we showed that TAES-induced sedation was accompanied by a reduction in alpha band power and an increment in delta band power. Permutation entropy was lower during the TAES session, which suggested that TAES reduced the complexity of the EEG signal. Moreover, a significant reduction in the global strength of brain functional connections was observed during TAES. These findings suggest that TAES alone can induce observable sedative effects, and this sedation effect is accompanied by changes in brain activities that have shown to be correlated with consciousness.
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http://dx.doi.org/10.1177/1550059420976303DOI Listing
December 2020

Lycorine hydrochloride inhibits cell proliferation and induces apoptosis through promoting FBXW7-MCL1 axis in gastric cancer.

J Exp Clin Cancer Res 2020 Oct 30;39(1):230. Epub 2020 Oct 30.

State Key Laboratory of Silkworm Genome Biology, College of Biotechnology, Southwest University, #1, Tiansheng Rd., Beibei District, Chongqing, 400716, China.

Background: Lycorine hydrochloride (LH), an alkaloid extracted from the bulb of the Lycoris radiata, is considered to have anti-viral, anti-malarial, and anti-tumorous effects. At present, the underlying mechanisms of LH in gastric cancer remain unclear. MCL1, an anti-apoptotic protein of BCL2 family, is closely related to drug resistance of tumor. Therefore, MCL1 is considered as a potential target for cancer treatment.

Methods: The effect of LH on gastric cancer was assessed in vitro (by MTT, BrdU, western blotting…) and in vivo (by immunohistochemistry).

Results: In this study, we showed that LH has an anti-tumorous effect by down-regulating MCL1 in gastric cancer. Besides, we unveiled that LH reduced the protein stability of MCL1 by up-regulating ubiquitin E3 ligase FBXW7, arrested cell cycle at S phase and triggered apoptosis of gastric cancer cells. Meanwhile, we also demonstrated that LH could induce apoptosis of the BCL2-drug-resistant-cell-lines. Moreover, PDX (Patient-Derived tumor xenograft) model experiment proved that LH combined with HA14-1 (inhibitor of BCL2), had a more significant therapeutic effect on gastric cancer.

Conclusions: The efficacy showed in our data suggests that lycorine hydrochloride is a promising anti-tumor compound for gastric cancer.
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http://dx.doi.org/10.1186/s13046-020-01743-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7602321PMC
October 2020

PTN-PTPRZ signalling is involved in deer antler stem cell regulation during tissue regeneration.

J Cell Physiol 2021 May 28;236(5):3752-3769. Epub 2020 Oct 28.

Faculty of Dentistry, Sir John Walsh Research Institute, University of Otago, Dunedin, New Zealand.

A growing deer antler contains a stem cell niche that can drive endochondral bone regeneration at up to 2 cm/day. Pleiotrophin (PTN), as a multifunctional growth factor, is found highly expressed at the messenger RNA level within the active antler stem cell tissues. This study aims to map the expression patterns of PTN protein and its receptors in a growing antler and investigate the effects of PTN on antler stem cells in vitro. Immunohistochemistry was employed to localise PTN/midkine (MDK) and their functional receptors, protein tyrosine phosphatase receptor type Z (PTPRZ), anaplastic lymphoma kinase (ALK), NOTCH2, and integrin α β on serial slides of the antler growth centre. PTN was found to be the dominantly expressed growth factor in the PTN/MDK family. High expression of PTPRZ and ALK co-localised with PTN was found suggesting a potential interaction. The high levels of PTN and PTPRZ reflected the antler stem cell activation status during the regenerative process. When antler stem cells were cultured in vitro under the normoxic condition, no PTN protein was detected and exogenous PTN did not induce differentiation or proliferation but rather stem cell maintenance. Collectively, the antler stem cell niche appears to upregulate PTN and PTPRZ in vivo, and PTN-PTPRZ signalling may be involved in regulating antler stem cell behaviour during rapid antler regeneration.
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http://dx.doi.org/10.1002/jcp.30115DOI Listing
May 2021

LINC01087 is Highly Expressed in Breast Cancer and Regulates the Malignant Behavior of Cancer Cells Through miR-335-5p/Rock1.

Onco Targets Ther 2020 30;13:9771-9783. Epub 2020 Sep 30.

Inner Mongolia Key Laboratory of Mongolian Medicine Pharmacology for Cardio-Cerebral Vascular System, Tongliao, Inner Mongolia, People's Republic of China.

Purpose: Long non-coding RNA is involved in the genesis and development of various tumors, and it has been found through database screening that LINC01087 is highly expressed in breast cancer (BC), but mechanisms of LINC01087 in BC are still under investigation. Therefore, this study aimed to study relevant mechanisms of LINC01087 in BC to provide potential therapeutic targets for the disease in clinic practice.

Patients And Methods: The qRT-PCR assay was applied to determine the LINC01087 expression in BC, and the cell counting kit-8 (CCK8) assay, transwell assay, and flow cytometry were used to analyze the proliferation, apoptosis, and invasion of breast cancer cells (BCCs), respectively. The Western blot assay was used to determine the ROCK1 expression, and the luciferase reporter gene assay, RNA-binding protein immunoprecipitation (RIP), and RNA pull-down assays were applied to study the interaction between LINC01087 and miR-335-5p. Moreover, tumor xenotransplantation was conducted in nude mice to explore the effects of LINC01087 on BCCs.

Results: The qRT-PCR assay revealed that the LINC01087 expression in BC tissues was higher than that in corresponding tumor-adjacent tissues, and survival analysis revealed an unfavorable prognosis of patients with high expression of LINC01087. Down-regulation of LINC01087 could slow down the proliferation, invasion, and migration of BCCs and accelerate apoptosis of them in vitro. Luciferase reporter gene assay results revealed that LINC01087 enhanced the expression of ROCK1 by regulating miR-335-5p, and LINC01087 could be adopted as a miR-335-5p sponge to inhibit ROCK1 expression.

Conclusion: LINC01087 is overexpressed in cases with BC, and patients with high expression of it suffer a poor survival. Furthermore, LINC01087 can act as a miR-335-5p sponge to affect the expression of ROCK1 and affect the invasion and migration of BCCs.
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http://dx.doi.org/10.2147/OTT.S255994DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7533226PMC
September 2020
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