Publications by authors named "Zhe Sun"

493 Publications

To improve the predictions of binding residues with DNA, RNA, carbohydrate, and peptide via multi-task deep neural networks.

IEEE/ACM Trans Comput Biol Bioinform 2021 Oct 12;PP. Epub 2021 Oct 12.

Motivation: The interactions of proteins with DNA, RNA, peptide, and carbohydrate play key roles in various biological processes. The studies of uncharacterized proteinmolecules interactions could be aided by accurate predictions of residues that bind with partner molecules. However, the existing methods for predicting binding residues on proteins remain of relatively low accuracies due to the limited number of complex structures in databases. As different types of molecules partially share chemical mechanisms, the predictions for each molecular type should benefit from the binding information with other molecules types.

Results: In this study, we employed a multiple task deep learning strategy to develop a new sequence-based method for simultaneously predicting binding residues/sites with multiple important molecule types named MTDsite. By combining four training sets for DNA, RNA, peptide, and carbohydrate-binding proteins, our method yielded accurate and robust predictions with AUC values of 0.852, 0836, 0.758, and 0.776 on their respective independent test sets, which are 0.52 to 6.6% better than other state-of-the-art methods. To my best knowledge, this is the first method using multi-task framework to predict multiple molecular binding sites simultaneously.

Availability: http://biomed.nscc-gz.cn/server/MTDsite/ Contact: [email protected]
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http://dx.doi.org/10.1109/TCBB.2021.3118916DOI Listing
October 2021

Revealing photon transmission in an ultraviolet reactor: Advanced approaches for measuring fluence rate distribution in water for model validation.

J Environ Sci (China) 2021 Dec 21;110:169-177. Epub 2021 Apr 21.

Key Laboratory of Drinking Water Science and Technology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

Fluence rate (FR) distribution (optical field) is of great significance in the optimal design of ultraviolet (UV) reactors for disinfection or oxidation processes in water treatment. Since the 1970s, various simulation models have been developed, which can be combined with computational fluidic dynamic software to calculate the fluence delivered in a UV reactor. These models strive for experimental validation and further improvement, which is a major challenge for UV technology in water treatment. Herein, a review of the simulation models of the FR distribution in a UV reactor and the applications of the current main experimental measurement approaches including conventional flat-type UV detector, spherical actinometer, and micro-fluorescent silica detector (MFSD), is presented. Moreover, FR distributions in a UV reactor are compared between various simulation models and MFSD measurements. In addition, the main influential factors on the FR distribution, including inner-wall reflection, refraction and shadowing effects of adjacent lamps, and turbidity effect are discussed, which is helpful for improving the accuracy of the simulation models and avoiding dark regions in the reactor design. This paper provides an overview on the simulation models and measurement approaches for the FR distribution, which is helpful for the model selection in fluence calculations and gives high confidence on the optimal design of UV reactors in regard to present methods.
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http://dx.doi.org/10.1016/j.jes.2021.03.038DOI Listing
December 2021

A Fast Approach to Removing Muscle Artifacts for EEG with Signal Serialization Based Ensemble Empirical Mode Decomposition.

Entropy (Basel) 2021 Sep 6;23(9). Epub 2021 Sep 6.

College of Artificial Intelligence, Nankai University, Tianjin 300350, China.

An electroencephalogram (EEG) is an electrophysiological signal reflecting the functional state of the brain. As the control signal of the brain-computer interface (BCI), EEG may build a bridge between humans and computers to improve the life quality for patients with movement disorders. The collected EEG signals are extremely susceptible to the contamination of electromyography (EMG) artifacts, affecting their original characteristics. Therefore, EEG denoising is an essential preprocessing step in any BCI system. Previous studies have confirmed that the combination of ensemble empirical mode decomposition (EEMD) and canonical correlation analysis (CCA) can effectively suppress EMG artifacts. However, the time-consuming iterative process of EEMD may limit the application of the EEMD-CCA method in real-time monitoring of BCI. Compared with the existing EEMD, the recently proposed signal serialization based EEMD (sEEMD) is a good choice to provide effective signal analysis and fast mode decomposition. In this study, an EMG denoising method based on sEEMD and CCA is discussed. All of the analyses are carried out on semi-simulated data. The results show that, in terms of frequency and amplitude, the intrinsic mode functions (IMFs) decomposed by sEEMD are consistent with the IMFs obtained by EEMD. There is no significant difference in the ability to separate EMG artifacts from EEG signals between the sEEMD-CCA method and the EEMD-CCA method ( > 0.05). Even in the case of heavy contamination (signal-to-noise ratio is less than 2 dB), the relative root mean squared error is about 0.3, and the average correlation coefficient remains above 0.9. The running speed of the sEEMD-CCA method to remove EMG artifacts is significantly improved in comparison with that of EEMD-CCA method ( < 0.05). The running time of the sEEMD-CCA method for three lengths of semi-simulated data is shortened by more than 50%. This indicates that sEEMD-CCA is a promising tool for EMG artifact removal in real-time BCI systems.
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http://dx.doi.org/10.3390/e23091170DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8465074PMC
September 2021

Effects of Different Combinations of Sugar and Starch Concentrations on Ruminal Fermentation and Bacterial-Community Composition .

Front Nutr 2021 3;8:727714. Epub 2021 Sep 3.

Key Laboratory of Animal Nutrition and Feed Science of Jilin Province, Key Laboratory of Animal Production Product Quality and Security Ministry of Education, JLAU-Borui Dairy Science and Technology R&D Center, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.

High levels of starch is known to have positive effects on both energy supply and milk yield but increases the risk of rumen acidosis. The use of sugar as a non-structural carbohydrate could circumvent this risk while maintaining the benefits, but its effects and that of the simultaneous use of both sugar and starch are not as well-understood. This study aimed to evaluate the effects of different combinations of sugar and starch concentrations on ruminal fermentation and bacterial community composition in a 4 ×4 factorial experiment. Sixteen dietary treatments were formulated with 4 levels of sugar (6, 8, 10, and 12% of dietary dry matter), and 4 levels of starch (21, 23, 25, and 27% of dietary dry matter). Samples were taken at 0.5, 1, 3, 6, 12, and 24 h after cultivation to determine the disappearance rate of dry matter, rumen fermentation parameters and bacterial community composition. Butyric acid, gas production, and abundance were significantly influenced by the sugar level. The pH, acetic acid, and propionic acid levels were significantly influenced by starch levels. However, the interactive effect of sugar and starch was only observed on the rate of dry matter disappearance. Furthermore, different combinations of starch and sugar had different effects on volatile fatty acid production rate, gas production rate, and dry matter disappearance rate. The production rate of rumen fermentation parameters in the high sugar group was higher. Additionally, increasing the sugar content in the diet did not change the main phylum composition in the rumen, but significantly increased the relative abundance of Bacteroidetes and Firmicutes phyla, while the relative abundance of Proteobacteria was reduced. At the genus level, the high glucose group showed significantly higher relative abundance of Treponema ( < 0.05) and significantly lower relative abundance of Ruminobacter, Ruminococcus, and Streptococcus ( < 0.05). In conclusion, different combinations of sugar and starch concentrations have inconsistent effects on rumen fermentation characteristics, suggesting that the starch in diets cannot be simply replaced with sugar; the combined effects of sugar and starch should be considered to improve the feed utilization rate.
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http://dx.doi.org/10.3389/fnut.2021.727714DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8446659PMC
September 2021

Development and validation of a novel classification scheme for combining pathological T stage and log odds of positive lymph nodes for colon cancer.

Eur J Surg Oncol 2021 Sep 9. Epub 2021 Sep 9.

Department of Gastrointestinal Surgery, The Fourth Affiliated Hospital of China Medical University, Shenyang, 110032, China; Department of Gastrointestinal Surgery, Graduate School of Medicine, The University of Tokyo, Tokyo, 113-8655, Japan. Electronic address:

Aim: Log Odds of Positive Lymph Nodes (LODDS) have a better predictive ability than N stage for colon cancer. However, the prognostic value of developing a novel prognostic classification by combining T stage and LODDS (TLODDS) for colon cancer remains unknown. Therefore, in the present study, we aimed to develop a TLODDS classification for colon cancer, and assess whether or not the novel TLODDS classification could improve survival stratification by comparing its discrimination, model-fitting, and net benefits, with the American Joint Committee on Cancer (AJCC) Tumor/Node/Metastasis (TNM) classification.

Methods: 45,558 Western colon cancers were identified in the Surveillance, Epidemiology, and End Results database as a training set. A novel LODDS stage was established and patients with similar survival rates were grouped by combining T and LODDS stages to develop a novel TLODDS classification. The TLODDS classification was further assessed in a Chinese validation set of 3,515 colon cancers and an application set of 3,053 rectal cancers.

Results: We developed a novel TLODDS classification that incorporated 7 stages: stage I (T1LODDS1), IIA (T2LODDS1, T1LODDS2, T1LODDS3), IIB (T2LODDS2-3, T3LODDS1, T1LODDS4), IIC (T3LODDS2, T2LODDS4, T4aLODDS1), IIIA (T3LODDS3, T1-2LODDS5, T4bLODDS1, T4aLODDS2), IIIB (T3LODDS4-5, T4aLODDS3-4, T4bLODDS2) and IIIC (T4bLODDS3-5, T4aLODDS5). In the training set, it showed significantly better discrimination (area under the receiver operating characteristic (ROC) curve, 0.691 vs. 0.664, P < 0.001), better model-fitting (Akaike information criteria, 265,644 vs. 267,410), and superior net benefits, than the latest AJCC TNM classification. The predictive performance of the TLODDS classification was further validated in colon cancers and was successfully applied in rectal cancers with regards to both overall and disease-free survival.

Conclusions: The TLODDS classification has better discriminatory ability, model-fitting, and net benefits than the existing TNM classification, and represents an alternative to the current TNM classifications for colon and rectal cancers.
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http://dx.doi.org/10.1016/j.ejso.2021.09.005DOI Listing
September 2021

An optical sensing system with ratiometric and turn-off dual-mode of [email protected] nanosheets for the determination of HO and glucose based on a combination of first-order scattering, fluorescence, and second-order scattering.

Spectrochim Acta A Mol Biomol Spectrosc 2022 Jan 21;264:120299. Epub 2021 Aug 21.

Key Laboratory of Luminescence Analysis and Molecular Sensing, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China. Electronic address:

The optical sensor with ratiometric and turn-off dual modes is constructed to detect HO and glucose based on blue fluorescent carbon dots (CDs) and MnO nanosheets with great ability of fluorescence quenching and scattering. Employing [email protected] nanosheets nanocomposite as the probe, HO is detected by simultaneously collecting first-order scattering (FOS, 353.5 nm), fluorescence (440 nm), and second-order scattering (SOS, 710 nm) under the excitation of 350 nm. HO with strong oxidation property can etch the lamellar structure of MnO nanosheets into nano-fragments, which made the fluorescence of CDs in the system recover and the scattering intensity (FOS and SOS) of the system decrease significantly. Therefore, the optical sensor combined FOS and fluorescence signals in ratiometric mode, and SOS signal in turn-off mode to realize sensitive determination of HO. The linear ranges of ratiometric mode and turn-off mode for HO detection were 0.2-40 and 0.2-15 μM, respectively. And the limits of detection (LODs) of two modes were 73 and 104 nM, respectively. Furthermore, the sensor was also successfully applied to the detection of glucose which can react to produce HO. Satisfactorily, the LODs of this sensor for glucose detection were 95 and 113 nM for ratiometric mode and turn-off mode, respectively. This work not only provides a new method for the accurate detection of HO and glucose, but also extends a new idea for the study of the combination of scattering and fluorescence.
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http://dx.doi.org/10.1016/j.saa.2021.120299DOI Listing
January 2022

An optical sensing system with ratiometric and turn-off dual-mode of [email protected] nanosheets for the determination of HO and glucose based on a combination of first-order scattering, fluorescence, and second-order scattering.

Spectrochim Acta A Mol Biomol Spectrosc 2022 Jan 21;264:120299. Epub 2021 Aug 21.

Key Laboratory of Luminescence Analysis and Molecular Sensing, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China. Electronic address:

The optical sensor with ratiometric and turn-off dual modes is constructed to detect HO and glucose based on blue fluorescent carbon dots (CDs) and MnO nanosheets with great ability of fluorescence quenching and scattering. Employing [email protected] nanosheets nanocomposite as the probe, HO is detected by simultaneously collecting first-order scattering (FOS, 353.5 nm), fluorescence (440 nm), and second-order scattering (SOS, 710 nm) under the excitation of 350 nm. HO with strong oxidation property can etch the lamellar structure of MnO nanosheets into nano-fragments, which made the fluorescence of CDs in the system recover and the scattering intensity (FOS and SOS) of the system decrease significantly. Therefore, the optical sensor combined FOS and fluorescence signals in ratiometric mode, and SOS signal in turn-off mode to realize sensitive determination of HO. The linear ranges of ratiometric mode and turn-off mode for HO detection were 0.2-40 and 0.2-15 μM, respectively. And the limits of detection (LODs) of two modes were 73 and 104 nM, respectively. Furthermore, the sensor was also successfully applied to the detection of glucose which can react to produce HO. Satisfactorily, the LODs of this sensor for glucose detection were 95 and 113 nM for ratiometric mode and turn-off mode, respectively. This work not only provides a new method for the accurate detection of HO and glucose, but also extends a new idea for the study of the combination of scattering and fluorescence.
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http://dx.doi.org/10.1016/j.saa.2021.120299DOI Listing
January 2022

Stable and twisted 5,6:12,13-dinaphthozethrene from angular π-extension.

Chem Commun (Camb) 2021 Sep 23;57(76):9712-9715. Epub 2021 Sep 23.

Institute of Molecular Plus, Tianjin Key Laboratory of Molecular Optoelectronic Sciences, Tianjin university, 92 Weijin Road, Tianjin 300072, China.

Herein, we describe a concise and efficient synthesis of an angularly extended stable zethrene derivative 1, designed to have more benzenoid rings in the closed-shell resonance form. This compound exhibited enantiomeric structures in the solid state derived from the benzo[4]helicene structure and rapid interconversion in solution. Its far-red absorption, near-infrared emission and amphoteric redox properties were also revealed.
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http://dx.doi.org/10.1039/d1cc04113dDOI Listing
September 2021

Coexistent Integer Charge Transfer and Charge Transfer Complex in F4-TCNQ-Doped PTAA for Efficient Flexible Organic Light-Emitting Diodes.

J Phys Chem Lett 2021 Sep 31;12(35):8533-8540. Epub 2021 Aug 31.

Key Laboratory of Display Materials and Photoelectric Devices, Ministry of Education; Tianjin Key Laboratory of Photoelectric Materials and Devices; National Demonstration Center for Experimental Function Materials Education; School of Materials Science and Engineering, Tianjin University of Technology, Tianjin 300384, P. R. China.

Understanding the mechanism of interaction between organic polymers and dopants is of great significance to further enhance the performances of flexible electronics. Here, the two doping mechanisms of charge transfer complex (CTC) and integer charge transfer (ICT) are found to coexist in p-π conjugated PTAA doped with the strong acceptor F4-TCNQ, and their correlation is affected by the HJ-aggregate state of the doped polymer. The growth of the J-aggregate caused by the increase of CTC would lead to a corresponding formation of ICT. The doping efficiency was dominated by the CTC/ICT ratio. On the basis of the analysis of the optical, electrical, and morphological properties of PTAA:F4-TCNQ films, we optimized the CTC/ICT ratio to achieve the efficient hole transport layers that are used in solution-processed flexible phosphorescent organic light-emitting diodes with p-i-n structure. The optimal device presents a very high current efficiency (CE) of 31.12 cd/A and a low turn-on voltage of 3.6 V.
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http://dx.doi.org/10.1021/acs.jpclett.1c02281DOI Listing
September 2021

Correlation of oxidative stress-related indicators with milk composition and metabolites in early lactating dairy cows.

Vet Med Sci 2021 Aug 29. Epub 2021 Aug 29.

College of Life Science, Jilin Agricultural University, Changchun, P. R. China.

Background: In highly intensive dairy farms, cows often suffer from metabolic disorders that cause severe oxidative stress.

Objectives: This study aimed to observe correlations and associations of oxidative stress-related indicators with milk compositions and metabolites.

Methods: Twenty-two multiparous Holstein dairy cows in early lactation were randomly selected from a commercial dairy farm. The morning milk was collected for composition and metabolites analysis. Blood was sampled via the tail vein to analyze oxidative stress-related indicators (reactive oxygen species, ROS; catalase, CAT; superoxide dismutase, SOD; glutathione peroxidase, GPX; malondialdehyde, MDA) and metabolites.

Results: Results showed that ROS were positively correlated with CAT, GPX, SOD, and MDA. However, the levels of CAT, GPX, and SOD were negatively related to milk fat (P  <  0.05). Nineteen serum and 7 milk metabolites were selected from detectable metabolites according to their correlations with ROS, CAT, GPX, and SOD (P  <  0.05). Metabolic pathway analysis and the Kyoto Encyclopedia of Genes and Genomes (KEGG) database revealed that these metabolites are primarily involved in the metabolic pathways of carbohydrates and amino acids.

Conclusions: This study gave us a better understanding on oxidative stress that ROS not only increased oxidative damage (MDA) in dairy cows, but also altered some metabolites involved in amino acid and carbohydrate metabolism.
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http://dx.doi.org/10.1002/vms3.615DOI Listing
August 2021

Effective Magnetic MOFs Adsorbent for the Removal of Bisphenol A, Tetracycline, Congo Red and Methylene Blue Pollutions.

Nanomaterials (Basel) 2021 Jul 26;11(8). Epub 2021 Jul 26.

National Special Superfine Powder Engineering Research Center of China, School of Chemistry and Chemical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.

A magnetic metal-organic frameworks adsorbent ([email protected](Al)) was prepared by a typical solvothermal method for the removal of bisphenol A (BPA), tetracycline (TC), congo red (CR), and methylene blue (MB). The prepared [email protected](Al) composite adsorbent was well characterized by scanning electron microscope (SEM), transmission electron microscope (TEM), X-ray diffraction (XRD), and fourier transform infrared spectrometer (FTIR). The influence of adsorbent quantity, adsorption time, pH and ionic strength on the adsorption of the mentioned pollutants were also studied by a UV/Vis spectrophotometer. The adsorption capacities were found to be 160.9 mg/g for BPA, 47.8 mg/g for TC, 234.4 mg/g for CR, 70.8 mg/g for MB, respectively, which is superior to the other reported adsorbents. The adsorption of BPA, TC, and CR were well-fitted by the Langmuir adsorption isotherm model, while MB followed the Freundlich model, while the adsorption kinetics data of all pollutants followed the pseudo-second-order kinetic models. The thermodynamic values, including the enthalpy change (Δ°), the Gibbs free energy change (Δ°), and entropy change (Δ°), showed that the adsorption processes were spontaneous and exothermic entropy-reduction process for BPA, but spontaneous and endothermic entropy-increasing processes for the others. The [email protected](Al) was also found to be easily separated after external magnetic field, can be a potential candidate for future water treatment.
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http://dx.doi.org/10.3390/nano11081917DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8398004PMC
July 2021

Amido-Functionalized Magnetic Metal-Organic Frameworks Adsorbent for the Removal of Bisphenol A and Tetracycline.

Front Chem 2021 6;9:707559. Epub 2021 Aug 6.

National Special Superfine Powder Engineering Research Center of China, School of Chemistry and Chemical Engineering, Nanjing University of Science and Technology, Nanjing, China.

In this paper, amido-functionalized MOFs with core/shell magnetic particles ([email protected](Al)-NH) was prepared by the solvothermal method and characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM), Fourier transform infrared (FT-IR), Vibrating Sample Magnetometer (VSM) and UV/VIS spectrophotometer. The influence of different factors on the adsorption effect of the pollutant, including adsorbent amounts, adsorption time, ionic strength and pH, were explored. It was found that the amine-decorated [email protected](Al)-NH were efficient for removal of contaminant, with the adsorption capacity for bisphenol A (234.1 mg/g) and tetracycline (84.8 mg/g) under the optimized conditions. The adsorption kinetics and the equilibrium adsorption data indicated that the adsorption process of BPA and TC was more compatible with the pseudo-second-order kinetic model and the Langmuir model, respectively. The thermodynamic values show the adsorption of the mentioned contaminant was spontaneous and endothermic. Moreover, the [email protected](Al)-NH adsorbent had good regeneration and reusability capacity after five cyclic utilization. All these results show [email protected](Al)-NH adsorbent could be a potential candidate for future water purification.
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http://dx.doi.org/10.3389/fchem.2021.707559DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8377470PMC
August 2021

K-80003 Inhibition of Macrophage Apoptosis and Necrotic Core Development in Atherosclerotic Vulnerable Plaques.

Cardiovasc Drugs Ther 2021 Aug 19. Epub 2021 Aug 19.

Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiao Tong University, Xuhui Distinct, 241 West Huaihai Road, Shanghai, China.

Purpose: Macrophage apoptosis coupled with a defective phagocytic clearance of the apoptotic cells promotes plaque necrosis in advanced atherosclerosis, which causes acute atherothrombotic vascular disease. Nonsteroidal anti-inflammatory drug sulindac derivative K-80003 treatment was previously reported to dramatically attenuate atherosclerotic plaque progression and destabilization. However, the underlying mechanisms are not fully understood. This study aimed to determine the role of K-80003 on macrophage apoptosis and elucidate the underlying mechanism.

Methods: The mouse model of vulnerable carotid plaque in ApoE mice was developed in vivo. Consequently, mice were randomly grouped into two study groups: the control group and the K-80003 group (30 mg/kg/day). Samples of carotid arteries were collected to determine atherosclerotic necrotic core area, cellular apoptosis, and oxidative stress. The effects of K-80003 on RAW264.7 macrophage apoptosis, oxidative stress, and autophagic flux were also examined in vitro.

Results: K-80003 significantly suppressed necrotic core formation and inhibited cellular apoptosis of vulnerable plaques. K-80003 can also inhibit 7-ketocholesterol-induced macrophage apoptosis in vitro. Furthermore, K-80003 inhibited intraplaque cellular apoptosis mainly through the suppression of oxidative stress, which is a key cause of advanced lesional macrophage apoptosis. Mechanistically, K-80003 prevented 7-ketocholesterol-induced impairment of autophagic flux in macrophages, evidenced by the decreased LC3II and SQSTM1/p62 expression, GFP-RFP-LC3 cancellation upon K-80003 treatment.

Conclusion: Inhibition of macrophage apoptosis and necrotic core formation by autophagy-mediated reduction of oxidative stress is one mechanism of the suppression of plaque progression and destabilization by K-80003.
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http://dx.doi.org/10.1007/s10557-021-07237-4DOI Listing
August 2021

Interaction Regulation Between Ionomer Binder and Catalyst: Active Triple-Phase Boundary and High Performance Catalyst Layer for Anion Exchange Membrane Fuel Cells.

Adv Sci (Weinh) 2021 Oct 2;8(19):e2101744. Epub 2021 Aug 2.

College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou, 215123, China.

As one of the most crucial components, the catalyst layer (CL) plays a critical role in the performance of anion exchange membrane fuel cells (AEMFCs). However, the effect of the structural evolution of ionomer binder on the micromorphology and catalytic activity of CL is yet to be clarified. In this study, pyrrolidinum and quaternary ammonium cations are attached to the polyphenylene oxide (PPO) backbone through flexible spacer units (five, seven, or nine carbon atoms) with different terminal alkyl groups. The Van der Waals force and electrostatic repulsion between the ionomer binder and catalyst are regulated through the flexible spacer units and terminal alkyl groups to alleviate the agglomeration of catalyst particles and acquire a high catalytic activity. To evaluate the electrochemical stability of the cationic groups, the alkaline stability of the ionomer binder is tested under a constant voltage to simulate the true operational environment of the fuel cells. The results reveal that the degradation of the cation groups of ionomer binder is accelerated under a constant voltage condition. This phenomenon in neglect earlier, may serve as a useful reference for the synthesis and performance enhancement of ionomer binders.
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http://dx.doi.org/10.1002/advs.202101744DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8498875PMC
October 2021

Intraperitoneal Chemotherapy Using Fluorouracil Implants Combined With Radical Resection and Postoperative Adjuvant Chemotherapy for Stage III Gastric Cancer: A Multi-Center, Randomized, Open-Label, Controlled Clinical Study.

Front Oncol 2021 8;11:670651. Epub 2021 Jul 8.

Department of Surgical Oncology and General Surgery, The First Hospital of China Medical University, Shenyang, China.

Background: Reducing peritoneal recurrence after radical surgery is an important choice to improve the prognosis of patients with advanced gastric cancer. Intraoperative intraperitoneal chemotherapy has the potential to be a promising treatment strategy. In the present study, we conducted a multi-center, randomized, controlled clinical study to evaluate the efficacy and safety of intraoperative intraperitoneal chemotherapy using sustained-release fluorouracil implants plus radical gastrectomy and adjuvant chemotherapy for cTNM stage III gastric cancer.

Methods: The patients were randomized into intraperitoneal chemotherapy group (sustained-release fluorouracil implants administration after standard D2 radical gastrectomy, and followed by XELOX adjuvant chemotherapy) and control group (standard D2 radical gastrectomy, and followed by XELOX adjuvant chemotherapy). A total of 122 patients from three centers were enrolled from September 2015 to February 2017.

Results: One hundred and two eligible patients completed the treatment course. The median follow-up time was 41.7 months (36.1-52.9 months). The 3-year progression-free survival rate and overall survival of patients in the intraperitoneal chemotherapy group were 43.9% and 49.1%, respectively, which were significantly better than those of the control group, 31.0% and 38.4%. In the intraperitoneal chemotherapy group, the number of cases with peritoneal recurrence was significantly less than that of the control group, 9 cases (17.3%) 19 cases (44.2%). There were neither significant differences between the groups in the incidence of hematogenous metastasis, lymph node metastasis, nor local metastasis.

Conclusion: For cTNM stage III gastric cancer, intraoperative sustained-release fluorouracil implants after radical resection combined with postoperative adjuvant chemotherapy, could significantly reduce the risk of peritoneal recurrence and prolong PFS. https://clinicaltrials.gov/, identifier (NCT02269904).
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http://dx.doi.org/10.3389/fonc.2021.670651DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8298064PMC
July 2021

A Study of the Brain Network Connectivity in Visual-Word Pairing Associative Learning and Episodic Memory Reactivating Task.

Comput Intell Neurosci 2021 8;2021:5579888. Epub 2021 Jul 8.

Computational Engineering Applications Unit, Head Office for Information Systems and Cybersecurity, RIKEN, Wako-Shi, Japan.

Episodic memory allows a person to recall and mentally reexperience specific episodes from one's personal past. Studies of episodic memory are of great significance for the diagnosis and the exploration of the mechanism of memory generation. Most of the current studies focus on certain brain regions and pay less attention to the interrelationship between multiple brain regions. To explore the interrelationship in the brain network, we use an open fMRI dataset to construct the brain functional connectivity and effective connectivity network. We establish a binary directed network of the memory when it is reactivated. The binary directed network shows that the occipital lobe and parietal lobe have the most causal connections. The number of edges starting from the superior parietal lobule is the highest, with 49 edges, and 31 of which are connected to the occipital cortex. This means that the interaction between the superior parietal lobule and the occipital lobe plays the most important role in episodic memory, and the superior parietal lobule plays a more causal role in causality. In addition, memory regions such as the precuneus and fusiform also have some edges. The results show that the posterior parietal cortex plays an important role of hub node in the episodic memory network. From the brain network model, more information can be obtained, which is conducive to exploring the brain's changing pattern in the whole memory process.
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http://dx.doi.org/10.1155/2021/5579888DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8285180PMC
July 2021

A review of the fluence determination methods for UV reactors: Ensuring the reliability of UV disinfection.

Chemosphere 2021 Jul 9;286(Pt 1):131488. Epub 2021 Jul 9.

Key Laboratory of Drinking Water Science and Technology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 18 Shuang-qing Road, Beijing, 100085, China; University of Chinese Academy of Sciences, 19 Yu-quan Road, Beijing, 100049, China. Electronic address:

Ultraviolet (UV) is a green and effective technique that has been widely applied in water disinfection. The reliability of UV disinfection is an important issue, in which the aim is to ensure the delivery of adequate real-time fluence in a UV reactor. Unlike chemical disinfection systems whose disinfection dose can be directly measured with disinfectant residuals, UV is a physical process and the determination of fluence is complicated in practical reactors. To date, several fluence determination methods have been developed, including conventional methods such as biodosimetry and model simulation, as well as emerging methods such as dyed microsphere method and the model-detector method. However, a systematic and comprehensive review of these methods is still needed to discuss the attributes and application scenarios of each method. In this review, we summarized the principal theories, procedures, applications, and pros/cons of these fluence determination methods. Further, the selection and application of appropriate fluence determination methods were discussed based on different purposes (e.g., feedbacks for reactor design, evidence for third-party validation, as well as on-site determination and long-term monitoring of fluence). Overall, this review could provide useful information and new insights regarding the application of current fluence determination methods to ensure the reliability of UV disinfection.
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http://dx.doi.org/10.1016/j.chemosphere.2021.131488DOI Listing
July 2021

LOG-CONTRAST REGRESSION WITH FUNCTIONAL COMPOSITIONAL PREDICTORS: LINKING PRETERM INFANT'S GUT MICROBIOME TRAJECTORIES TO NEUROBEHAVIORAL OUTCOME.

Ann Appl Stat 2020 Sep 18;14(3):1535-1556. Epub 2020 Sep 18.

University of Connecticut.

The neonatal intensive care unit (NICU) experience is known to be one of the most crucial factors that drive preterm infant's neurodevelopmental and health outcome. It is hypothesized that stressful early life experience of very preterm neonate is imprinting gut microbiome by the regulation of the so-called brain-gut axis, and consequently, certain microbiome markers are predictive of later infant neurodevelopment. To investigate, a preterm infant study was conducted; infant fecal samples were collected during the infants' first month of postnatal age, resulting in functional compositional microbiome data, and neurobehavioral outcomes were measured when infants reached 36-38 weeks of post-menstrual age. To identify potential microbiome markers and estimate how the trajectories of gut microbiome compositions during early postnatal stage impact later neurobehavioral outcomes of the preterm infants, we innovate a sparse log-contrast regression with functional compositional predictors. The functional simplex structure is strictly preserved, and the functional compositional predictors are allowed to have sparse, smoothly varying, and accumulating effects on the outcome through time. Through a pragmatic basis expansion step, the problem boils down to a linearly constrained sparse group regression, for which we develop an efficient algorithm and obtain theoretical performance guarantees. Our approach yields insightful results in the preterm infant study. The identified microbiome markers and the estimated time dynamics of their impact on the neurobehavioral outcome shed lights on the linkage between stress accumulation in early postnatal stage and neurodevelpomental process of infants.
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http://dx.doi.org/10.1214/20-aoas1357DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8218926PMC
September 2020

Spatiotemporal variations and regional differences in air temperature in the permafrost regions in the Northern Hemisphere during 1980-2018.

Sci Total Environ 2021 Oct 8;791:148358. Epub 2021 Jun 8.

Cryosphere Research Station on Qinghai-Xizang Plateau, State Key Laboratory of Cryospheric Sciences, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou 730000, China.

Surface air temperature is an important factor for the permafrost thermal state in the Northern Hemisphere. It is therefore necessary to understand the variations and regional differences in air temperature to determine the interactions between permafrost degradation and climate change. In this study, we used observational data from the National Centers for Environmental Information, the China Meteorological Administration, and the World Data Centre for Meteorology to quantitatively analyze the variations and regional differences in air temperature from 1980 to 2018. The results demonstrated that the annual mean air temperatures were low in continuous permafrost regions and high in sporadic and isolated permafrost regions, with a significant warming rate of 0.371 ± 0.086 °C/decade. Air temperatures warmed the slowest during the winter and fastest during the spring, and no "warming hiatus" was observed in the permafrost regions of the Northern Hemisphere. The spatial patterns of freezing degree-days (FDDs) and thawing degree-days (TDDs) had different spatial characteristics. The decreasing rate of FDDs was -6.97 °C·days/year, while the increasing rate of TDDs was 6.4 °C·days/year. The air temperatures and warming trends had largely regional differences with respect to high latitude, transitional, and high altitude permafrost regions. Air temperature and its warming trend was the highest in high altitude regions. In addition, air temperature warming trends gradually decreased from the continuous permafrost zone to the island permafrost zone. The FDDs had a significant decreasing trend from the continuous permafrost zone to the island permafrost zone, whereas TDDs exhibited the opposite trend. The results indicate that the air temperature warming rate in the permafrost regions was approximately 2.0 times that of the global warming rate, and 1.3 times the global land warming rate from 1980 to 2018. These findings offer a perspective on the differences in permafrost and its thermal state across different regions under climate change.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148358DOI Listing
October 2021

Removal of disinfection by-product precursors in drinking water treatment processes: Is fluorescence parallel factor analysis a promising indicator?

J Hazard Mater 2021 09 4;418:126298. Epub 2021 Jun 4.

Key Laboratory of Drinking Water Science and Technology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 18 Shuang-qing Road, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

This work investigated the removal efficiency of disinfection by-product (DBP) precursors by different drinking water treatment processes and evaluated the feasibility of using fluorescence components removal as an indicator. A four-component (including tryptophan-like, protein-bound, tyrosine-like, and humic-like components) parallel factor analysis model was developed basing on 288 fluorescence excitation-emission matrices. Among all treatment processes, coagulation-sedimentation process showed the best performance, with mean removal ratios of 30% in total fluorescence intensity and 31% in total formation potential (FP) of DBPs, respectively. It preferentially removed humic-like component C4 (43%). Advanced treatment processes were less effective in comparison. Ozone and biological activated carbon (BAC) combined process reduced 20% of total fluorescence intensity, while ultrafiltration process reduced < 3%. Ozonation and BAC filtration preferentially removed free amino acids (i.e., C1 and C3) and protein-bound (i.e., C2) components, with mean removal ratios of 12% and 17%, respectively. Significant correlations (p < 0.01, double-tailed) were observed between four fluorescence components removal and FPs reduction of three trihalomethanes, dichloroacetonitrile (DCAN), and 1,1-dichloropropanone (1,1-DCP). Specifically, the correlation coefficients for three trihalomethanes and 1,1-DCP followed the order of C4 > C1 > C2 > C3, while the order for DCAN was C2 > C4 > C1 > C3.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126298DOI Listing
September 2021

Silencing lncRNA GAS5 alleviates apoptosis and fibrosis in diabetic cardiomyopathy by targeting miR-26a/b-5p.

Acta Diabetol 2021 Nov 6;58(11):1491-1501. Epub 2021 Jun 6.

The Second Department of Endocrinology, The First Hospital of Qiqihar & Affiliated Qiqihar Hospital, Southern Medical University, No. 30 Park Road, Longsha, Qiqihar, 161005, Heilongjiang, People's Republic of China.

Background: LncRNA GAS5 is associated with high glucose-induced cardiomyocyte injury, but its role in diabetic cardiomyopathy (DCM) remains unclear.

Methods: Mice were administered with streptozotocin to construct the diabetic model (DM). Primary mouse cardiomyocytes were isolated and treated with 30 mmol/L high glucose to mimic the diabetic condition in vitro. GAS5 expression was detected by quantitative reverse transcription polymerase chain reaction. The relationship between GAS5 and miR-26a/b-5p was determined by bioinformatic prediction, luciferase reporter assay and RNA immunoprecipitation assay. The cardiac function of diabetic mice was evaluated by two-dimensional echocardiography.

Results: GAS5 was significantly upregulated in diabetic cardiomyopathy both in vitro and in vivo. GAS5 knockdown and miR-26a/b-5p overexpression not only effectively attenuated myocardial fibrosis of diabetic mice in vivo but also inhibited high glucose-induced cardiomyocyte injury in vitro. miR-26a/b-5p was identified as a target of GAS5. GAS5 knockdown efficiently attenuated myocardial fibrosis and high glucose-induced cardiomyocyte injury through negatively regulating miR-26a/b-p.

Conclusion: Our study showed that GAS5 promotes DCM progression by regulating miR-26a/b-5p, suggesting that GAS5 might be a potential therapeutic target for DCM.
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http://dx.doi.org/10.1007/s00592-021-01745-3DOI Listing
November 2021

Effects of CeO and SbO on the Nonlinear Photochemical Process in Ultrashort Laser Gaussian-Bessel Beams Irradiated Photo-Thermo-Refractive Glass.

Micromachines (Basel) 2021 May 26;12(6). Epub 2021 May 26.

Electronic Information College, Northwestern Polytechnical University, Xi'an 710072, China.

Microfluidic chips and optical elements can be fabricated based on the nonlinear photosensitivity in photo-thermo-refractive (PTR) glass by controlling the growth of nanocrystals in the femtosecond (fs) laser-irradiated region. Here, we focus on CeO and SbO that play important roles in UV irradiation, experimentally investigate the effects of the dopants on the nonlinear photochemical process in PTR glass triggered by fs Gaussian-Bessel beams. The results show that the generation of Ag atoms and the Ag nanoparticles can be improved by CeO and SbO co-doping. Besides, each multivalent ion in PTR glass possibly participates in the electron transfer processes and contributes to the generation of Ag atoms. Finally, X-ray diffraction analysis reveals the precipitation of NaF nanocrystals with an average size of 10 to 12 nm after laser irradiation and thermal treatment, which is unrelated to the dopants.
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http://dx.doi.org/10.3390/mi12060615DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8229932PMC
May 2021

Chimeric HP-PRRSV2 containing an ORF2-6 consensus sequence induces antibodies with broadly neutralizing activity and confers cross protection against virulent NADC30-like isolate.

Vet Res 2021 May 27;52(1):74. Epub 2021 May 27.

College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, Jiangsu, China.

Due to the substantial genetic diversity of porcine reproductive and respiratory syndrome virus (PRRSV), commercial PRRS vaccines fail to provide sufficient cross protection. Previous studies have confirmed the existence of PRRSV broadly neutralizing antibodies (bnAbs). However, bnAbs are rarely induced by either natural infection or vaccination. In this study, we designed and synthesized a consensus sequence of PRRSV2 ORF2-6 genes (ORF2-6-CON) encoding all envelope proteins based on 30 representative Chinese PRRSV isolates. The ORF2-6-CON sequence shared > 90% nucleotide identities to all four lineages of PRRSV2 isolates in China. A chimeric virus (rJS-ORF2-6-CON) containing the ORF2-6-CON was generated using the avirulent HP-PRRSV2 JSTZ1712-12 infectious clone as a backbone. The rJS-ORF2-6-CON has similar replication efficiency as the backbone virus in vitro. Furthermore, pig inoculation and challenge studies showed that rJS-ORF2-6-CON is not pathogenic to piglets and confers better cross protection against the virulent NADC30-like isolate than a commercial HP-PRRS modified live virus (MLV) vaccine. Noticeably, the rJS-ORF2-6-CON strain could induce bnAbs while the MLV strain only induced homologous nAbs. In addition, the lineages of VDJ repertoires potentially associated with distinct nAbs were also characterized. Overall, our results demonstrate that rJS-ORF2-6-CON is a promising candidate for the development of a PRRS genetic engineered vaccine conferring cross protection.
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http://dx.doi.org/10.1186/s13567-021-00944-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8161975PMC
May 2021

The E2F1/USP11 positive feedback loop promotes hepatocellular carcinoma metastasis and inhibits autophagy by activating ERK/mTOR pathway.

Cancer Lett 2021 Aug 24;514:63-78. Epub 2021 May 24.

Department of Hepatobiliary and Pancreas Surgery, The Second Clinical Medical College, Jinan University (Shenzhen People's Hospital), Shenzhen, 518020, Guangdong, China; Department of Hepatobiliary and Pancreas Surgery, The First Affiliated Hospital, Southern University of Science and Technology, Shenzhen, 518020, Guangdong, China. Electronic address:

Deubiquitinase ubiquitin-specific protease 11 (USP11), a member of the deubiquitinating family, plays an important but still controversial role in cancer development. Namely, USP11 has been shown to promote the proliferation and metastasis of hepatocellular carcinoma (HCC), but the underlying molecular basis is poorly understood. This study aimed to unravel novel functions of USP11 in HCC, especially those related to autophagy. Here, EdU, migration and colony formation assays, and mouse models showed that USP11 played a crucial role in HCC cell proliferation and metastasis in vitro and in vivo. Results from co-immunoprecipitation and ubiquitination assays demonstrated that USP11 interacted with E2F1 and maintained E2F1 protein stability by removing its ubiquitin. Notably, E2F1 regulated USP11 expression at the transcriptional level. Thus, the E2F1/USP11 formed a positive feedback loop to promote the proliferation and migration of HCC cells. Moreover, E2F1/USP11 inhibited autophagy by regulating ERK/mTOR pathway. In addition, the combination treatment inhibition of USP11 and autophagy enhanced the apoptosis of HCC cells and inhibited the tumor growth in mice more effective than either treatment alone. Taken together, these results indicate that the E2F1/USP11 signal axis promotes HCC proliferation and metastasis and inhibits autophagy, which provides an experimental basis for the treatment of HCC.
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http://dx.doi.org/10.1016/j.canlet.2021.05.015DOI Listing
August 2021

Isomeric Dibenzoheptazethrenes for Air-Stable Organic Field-Effect Transistors.

Angew Chem Int Ed Engl 2021 Jul 15;60(29):16230-16236. Epub 2021 Jun 15.

Institute of Molecular Plus, Tianjin Key Laboratory of Molecular Optoelectronic Sciences, Tianjin university, 92 Weijin Road, Tianjin, 300072, China.

Singlet diradicaloids hold great potential as semiconductors for organic field-effect transistors (OFETs). However, their relative low material and device stabilities impede the practical applications. Here, to achieve balanced stability and performance, two isomeric dibenzoheptazethrene derivatives with singlet diradical character were synthesized in a concise manner. Benefitting from the aromatic stabilization, both compounds display a small diradical character and large singlet-triplet gap, as corroborated by variable-temperature electron paramagnetic resonance spectra, single-crystal analysis, and theoretical calculations. OFET devices based on single crystals showed a high hole mobility of 0.15 cm  V  s , which is the highest for zethrene-based semiconductors. Both isomers exhibited remarkable material stability in air-saturated solutions as well as excellent bias-stress and storage stability in device under ambient air.
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http://dx.doi.org/10.1002/anie.202105872DOI Listing
July 2021

Universal and Programmable Rolling Circle Amplification-CRISPR/Cas12a-Mediated Immobilization-Free Electrochemical Biosensor.

Anal Chem 2021 05 13;93(20):7499-7507. Epub 2021 May 13.

Key Laboratory of Luminescence Analysis and Molecular Sensing, School of Chemistry and Chemical Engineering, Southwest University, Tiansheng Road, BeiBei District, Chongqing 400715, P. R. China.

The development of a sensing platform with high sensitivity and specificity, especially programmability and universal applicability, for the detection of clinically relevant molecules is highly valuable for disease monitoring and confirmation but remains a challenge. Here, for the first time, we introduce the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system into an immobilization-free electrochemical biosensing platform for sensitively and specifically detecting the disease-related nucleic acids and small molecules. In this strategy, a modular rolling circle amplification (RCA) is designed to transform and amplify the target recognition event into the universal trigger DNA strand that is used as the trigger to activate the deoxyribonuclease activity of CRISPR/Cas12a for further signal amplification. The cleavage of the target-activated blocker probe allows the methylene blue-labeled reporter probes to be captured by the reduced graphene oxide-modified electrode, leading to an obviously increased electrochemical signal. We only need to simply tune the sequence for target recognition in RCA components, and this strategy can be flexibly applied to the highly sensitive and specific detection of microRNAs, Parvovirus B19 DNA, and adenosine-5'-triphosphate and the calculated limit of detection is 0.83 aM, 0.52 aM, and 0.46 pM, respectively. In addition, we construct DNA logic circuits (YES, NOT, OR, AND) of DNA inputs to experimentally demonstrate the modularity and programmability of the stimuli-responsive RCA-CRISPR/Cas12a system. This work broadens the application of the CRISPR/Cas12a system to the immobilization-free electrochemical biosensing platform and provides a new thinking for developing a robust tool for clinical diagnosis.
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http://dx.doi.org/10.1021/acs.analchem.1c00805DOI Listing
May 2021

The need for immunotherapy when neoadjuvant chemoradiotherapy achieves pathologic complete response in stage IIIB non-small cell lung cancer: a case report.

Ann Palliat Med 2021 Apr;10(4):4965-4969

Department of Thoracic Surgery, The First Affiliated Hospital of Dalian Medical University, Dalian, China.

The 5-year survival rate of patients with stage IIIB non-small cell lung cancer (NSCLC) range is 26%. Pathological complete response (pCR) is the best outcome after treatment for stage IIIB NSCLC. For patients with stage IIIB NSCLC, concurrent chemoradiotherapy with a curative intent is currently the standard treatment. For patients who respond to treatment, this is followed by consolidation immunotherapy with durvalumab. However, because of the complex and diverse nature of stage IIIB NSCLC, standard treatment is not necessarily suitable for all patients; rather, individualized and precise treatment can maximize the benefits of patients. Herein, we report a case of a patient with stage IIIB lung squamous cell carcinoma (SCC) treated with neoadjuvant chemoradiotherapy after receiving all 6 cycles of treatment, the patient underwent video-assisted thoracoscopic surgery (VATS) right upper lobectomy, right middle partial lobectomy, right lower partial lobectomy, and systematic mediastinal lymph node dissection. Postoperative pathological section results showed a pCR. The patient did not continue to use immunotherapy as a consolidation treatment after surgery. He remained disease free until the latest follow-up a half year later. This case has led us to doubt whether immunotherapy with durvalumab is still needed for patients with pCR. However, more clinical trials are needed to provide stronger evidence.
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http://dx.doi.org/10.21037/apm-21-708DOI Listing
April 2021

A highly virulent canine distemper virus strain isolated from vaccinated mink in China.

Virus Genes 2021 Jun 5;57(3):266-275. Epub 2021 May 5.

National Research Center for Veterinary Medicine, No.3 Cuiwei Road, High-Tech District, Luoyang, Henan, 471003, PR China.

An outbreak of canine distemper in 2017 in mink breeding farms (Shandong province, China) caused severe pneumonia, hardened footpads, and death in more than 5000 vaccinated animals. Sequencing of the hemagglutinin and fusion protein genes from the WH2 canine distemper virus (CDV) strain we isolated from the infected minks were clustered into the recently isolated CDV Asia-1 genotype group. The WH2 strain was distinct from the current vaccine strains, containing a novel potential N-glycosylation site in its hemagglutinin protein. It also contained amino acid mutations in the fusion protein gene (I87N, T110P and L386I), and the T110P mutation results in N-glycosylation site silencing. WH2 was highly virulent in both unvaccinated and vaccinated animals in our pathogenesis experiments. Immunohistochemistry results revealed positive staining of different organs in unvaccinated and vaccinated animals. The serum in vitro neutralizing antibody titers for the vaccinated mink group and a dog were higher for the WH2 strain than those of the HNly150520B strain (isolated from a dog). These findings indicate that the current commercial vaccines provide incomplete protection against WH2 challenge infections. Thus, a new vaccine strain is urgently needed to protect against variant CDV strains.
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http://dx.doi.org/10.1007/s11262-021-01837-wDOI Listing
June 2021

[The effect of low-magnitude high-frequency loading on peri-implant bone healing and implant osseointegration in Beagle dogs].

Shanghai Kou Qiang Yi Xue 2021 Feb;30(1):17-22

School of Stomatology, Zhejiang Chinese Medical University. Hangzhou 310053, Zhejiang Province, China.

Purpose: Low-magnitude, high-frequency(LMHF) loading has been confirmed to play an important role in bone healing. The present study aimed to evaluate the effect of LMHF loading applied directly to titanium dental implants on peri-implant bone healing and implant.

Methods: The mandibular premolars and molars were extracted from six male Beagle dogs. Three months post-extraction, six titanium implants (Aadva Standard Implant Narrow, 3.3 mm×8 mm) were inserted into the mandibular premolar and molar area (three implants per side) in each of the six dogs. In each animal, one side was randomly selected to undergo daily LMHF loading (experimental group), while the other side had no further intervention (control group). The loading was applied directly to the implant abutment using an individual jig and a custom-made loading device (8 μm,100 Hz). The implant stability quotient (ISQ) was tested every week. Three dogs were euthanized after 2 weeks, and three were euthanized after 8 weeks. Tissue samples were fixed and stained for micro-computed tomography(micro-CT) and histomorphometric analyses. The data were statistically analyzed using SPSS 13.0 software package.

Results: The experimental group had significantly increased peri-implant bone volume relative to tissue volume in region of interest 2 (100-500 μm) compared with the control group after 2 weeks of loading (P<0.05); however, there was no significant difference between the two groups after 8 weeks (P>0.05). The ISQ value and the micro-CT results did not differ between the two groups during the study period.

Conclusions: LMHF loading positively influences peri-implant bone healing and osseointegration in the early healing period.
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February 2021

Genetic Mutation Analysis in Small Cell Lung Cancer by a Novel NGS-Based Targeted Resequencing Gene Panel and Relation with Clinical Features.

Biomed Res Int 2021 5;2021:3609028. Epub 2021 Apr 5.

Department of Thoracic Surgery, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, China.

Background: Small cell lung cancer (SCLC) is an aggressive and invasive malignancy that presents at advanced clinical stage with no more effective treatments. Development of a method for its early detection would be useful, also new therapeutic target need to be discovered; however, there is a lack of information about its oncogenic driver gene mutations.

Objectives: We aim to identify the SCLC-related genomic variants that associate with clinical staging and serum protein biomarkers observed in other types of lung cancer.

Methods: We screened formalin-fixed paraffin-embedded (FFPE) biopsy tissues of 32 Chinese SCLC patients using the 303 oncogenic driver gene panel generated by Tiling PCR amplification sequencing (tPAS) and analyzed the patients' corresponding serum protein levels of CYFRA21-1 CEA, NSE, and SCCA.

Results: In total, we found 147 SCLC-related mutant genes, among these, three important genes (, , ) as well as five novel genes , , , , and that altogether occurred in 90% of patients. Furthermore, increased mutations to 6 genes (, , , , , ) significantly associated with higher serum NSE levels ( = 0.0016) and higher clinical stages II + III compared to stage I ( = 0.06).

Conclusions: Our panel is relatively reliable in detecting the oncogenic mutations of Chinese SCLC patients. Based on our findings, it may be possible to combine SCLC-related mutations and serum NSE for a simple detection of clinical staging.
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http://dx.doi.org/10.1155/2021/3609028DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8046547PMC
May 2021
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