Publications by authors named "Zhe Chen"

1,279 Publications

  • Page 1 of 1

Cilia regulate meiotic recombination in zebrafish.

J Mol Cell Biol 2022 Aug 18. Epub 2022 Aug 18.

Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, China.

Meiosis is essential for evolution and genetic diversity in almost all sexual eukaryotic organisms. The mechanisms of meiotic recombination, such as synapsis, have been extensively investigated. However, it is still unclear whether signals from the cytoplasm or even from outside of the cell can regulate the meiosis process. Cilia are microtubule-based structures that protrude from cell surface and function as signaling hubs to sense extracellular signals. Here, we reported an unexpected and critical role of cilia during meiotic recombination. During gametogenesis of zebrafish, cilia were specifically present in the prophase stages of both primary spermatocytes and primary oocytes. By developing a germ cell-specific CRISPR/Cas9 system, we demonstrated that germ cell-specific depletion of ciliary genes resulted in compromised double-strand break repair, reduced crossover formation, and increased germ cell apoptosis. Our study reveals a previously undiscovered role for cilia during meiosis and suggests that extracellular signals may regulate meiotic recombination via this particular organelle.
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http://dx.doi.org/10.1093/jmcb/mjac049DOI Listing
August 2022

Skin dose distributions between Stanford and rotational techniques in total skin electron therapy (TSET).

Med Phys 2022 Aug 16. Epub 2022 Aug 16.

Dept. of Radiation Oncology, Perelman Center of Advanced Medicine, Univ of Pennsylvania.

Purpose: Total skin electron therapy (TSET) has proven to be one of the most effective treatments for advanced-stage cutaneous T-cell lymphoma. Two most used techniques are the Stanford six-field and rotational techniques. This study compares patient skin dose distributions as a function of depth between these two techniques.

Methods: The EGSnrc system was used to simulate electron beams and calculate patient dose distributions. The calculations assumed the same patient standing on a platform and the patient's different postures were ignored for the Stanford technique in the comparison of dose distributions. The skin doses were analyzed as a function of skin depth-dose coverage and evaluated using dose-volume-histograms (DVH). The comparisons were performed in three realistic clinical settings in which dual-field were used for patients treated at extended distances of 316 cm and 500 cm, and a single field was used at 700 cm. In all cases the realistic patient treatment beam delivery geometry was simulated.

Results: Although small dose differences were observed in some local areas, no clinically significant differences were found in the patient 3D dose distributions between the Stanford and rotational techniques. Virtually the same DVH curves between two the techniques were observed for mean dose to skin depth of 0-5 mm, 5-10 mm and 10-15 mm from the skin surface, respectively. It is found that the skin depth dose coverage is 2 mm shallower for patient treatment at 500 cm compared to at 316 cm due to the additional air attenuation. However, very similar dose coverage and uniformity can be achieved at these two different extended treatment distances by adjusting the thickness of acrylic scatter plate. Adequate thickness of a scattering plate improves the skin dose uniformity.

Conclusion: Both the Stanford and rotational techniques deliver very similar skin dose coverage in DVH plots and only small differences are seen in local areas. It is worth to emphasize that the dose-volume histogram (DVH) is a graphical representation of the distribution of dose within a structure and it does not contain spatial information. Therefore, comparison of entire skin dose using DVH may mask some variations at different locations of the surface area. In addition, the comparison did not consider different patient postures of the Stanford technique. Including the different patient postures in the calculation may affect the result of doses to the limbs. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/mp.15907DOI Listing
August 2022

P and Cu Dual Sites on Graphitic Carbon Nitride for Photocatalytic CO2 Reduction to Hydrocarbon Fuels with High C2H6 Evolution.

Angew Chem Int Ed Engl 2022 Aug 15. Epub 2022 Aug 15.

Anhui Normal University, College of Chemistry and Materials Science, CHINA.

The light-driven CO2 reduction to multi-carbon products is especially meaningful, while the low efficiency of multi-electron transfer and sluggish C-C coupling greatly hinder its development. Herein, we report a photocatalyst comprising of P and Cu dual sites anchored on graphitic carbon nitride (P/Cu [email protected]), which achieves a high C2H6 evolution rate of 616.6 μmol g-1 h-1 in reducing CO2 to hydrocarbons. The detailed spectroscopic characterizations identify the formation of charge-enriched Cu sites, where the isolated P atoms serve as hole capture sites during photocatalysis. Theoretical simulations combined with in-situ FTIR measurement reveal a kinetically feasible for the formation of C-C coupling intermediate (*OC-COH) and confirm the favorable production of C2H6 on the P/Cu [email protected] photocatalyst. This work offers new insights into the photocatalyst design with atomic precision toward highly efficient photocatalytic CO2 conversion to high value-added carbon products.
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http://dx.doi.org/10.1002/anie.202210789DOI Listing
August 2022

Transcervical Exploration via the Posterolateral Approach to Extract a Fishbone Embedded in the Paraglottic Space.

Ear Nose Throat J 2022 Aug 13:1455613221116334. Epub 2022 Aug 13.

Department of Otolaryngology, 71069The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, P.R. China.

Ingested foreign bodies occasionally migrate to the paraglottic space. The external transcervical approach is almost always required to extract completely embedded foreign bodies. We report a case of an ingested fishbone embedded in the paraglottic space, which was successfully removed through transcervical exploration of the paraglottic space via the posterolateral approach. The posterolateral approach is safe and effective for the removal of foreign bodies completely embedded in the paraglottic space.
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http://dx.doi.org/10.1177/01455613221116334DOI Listing
August 2022

Preparation of 3D Printing PLGA Scaffold with BMP-9 and P-15 Peptide Hydrogel and Its Application in the Treatment of Bone Defects in Rabbits.

Contrast Media Mol Imaging 2022 31;2022:1081957. Epub 2022 Jul 31.

Department of Orthopedics, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, China.

Objective: To prepare a three-dimensional (3D) printing polylactic acid glycolic acid (PLGA) scaffold with bone morphogenetic protein-9 (BMP-9) and P-15 peptide hydrogel and evaluate its application in treating bone defects in rabbits.

Methods: 3D printing PLGA scaffolds were formed and scanned by electron microscopy. Their X-ray diffraction (XRD), degradation, and compressive strength were characterized. BMP-9 and P-15 hydrogels were prepared. Flow cytometry was used to detect apoptosis, and an electron microscope was used to evaluate cell adhesion to scaffolds. Alkaline phosphatase (ALP), type 1 collagen (Col-I), osteocalcin (OCN), runt-related transcription factor 2 (RUNX2), and osterix (SP7) were detected by western blotting. MicroCT was used to detect new bone formation, and bone tissue-related protein expressions were determined in the rabbit model with bone defects.

Results: The 3D printing scaffolds were cylindrical, and the inner diameter of the scaffolds was about 1 mm. The bread peak with wide distribution showed that the 3D printing only involved a physical change, which did not change the properties of the materials. The degradation rate of scaffolds was 9.38%, which met the requirements of properties of biological scaffolds. The water absorption of the support was about 9.09%, and the compressive strength was 15.83 N/mm. In the coculture of bone marrow mesenchymal stem cells (BMSCs) with scaffolds, the 2% polypeptide hydrogel showed the most obvious activity in promoting the differentiation of BMSCs. Flow cytometry showed that the 0% and 2% groups did not cause obvious apoptosis compared with the control group. Scaffolds with 2% and 4% polypeptide promoted the expression of ALP, COL-1, OCN, RUNX2, and Sp7 in BMSCs. experiments showed that the expression of ALP, COL-1, OCN, RUNX2, and Sp7 protein in the 2% polypeptide scaffold group increased significantly compared with the model group. MicroCT detection demonstrated that the 2% polypeptide scaffold had good bone repair ability.

Conclusion: The PLGA scaffolds combined with BMP-9 and P-15 peptide hydrogels had good biological and mechanical properties and could repair bone defects in rabbits.
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http://dx.doi.org/10.1155/2022/1081957DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9357721PMC
August 2022

Current status of microplastics and nanoplastics removal methods: Summary, comparison and prospect.

Sci Total Environ 2022 Aug 11:157991. Epub 2022 Aug 11.

State Key Laboratory of Food Science and Technology, Jiangnan University, No. 1800 Lihu Avenue, Wuxi 214122, Jiangsu Province, China; School of Food Science and Technology, Jiangnan University, No. 1800 Lihu Avenue, Wuxi 214122, Jiangsu Province, China; Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, No. 1800 Lihu Avenue, Wuxi 214122, Jiangsu Province, China. Electronic address:

In modern society, plastics also play an indispensable role in people's lives due to their various excellent properties. However, when these plastic products are discarded after being used, after being subjected to external influences, they will continue to be worn, damaged and degraded into micro- and nano-scale plastics, which are microplastics and nanoplastics (M/NPs). Although people's attention has been paid to M/NPs at present, the focus is still mainly on the detection and hazard of M/NPs, and how to remove M/NPs is relatively less popular. This review was written in order to draw the attention of more researchers to remove M/NPs. This review first briefly introduces the research background of M/NPs, and also shows the main analytical methods currently used for qualitative and quantitative M/NPs. Then, most of the current literature on the removal of M/NPs was collected, and they were classified, summarized, and introduced according to the classification of physical, physicochemical, and biological methods. The advantages and disadvantages of various methods are summarized, and they are also compared, which can help more researchers choose the appropriate method for research. In addition, the application scenarios of these methods are briefly introduced. Finally, some future research directions are proposed for the current research status of M/NPs removal. It is hoped that this will further promote the development on the method of removing M/NPs.
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http://dx.doi.org/10.1016/j.scitotenv.2022.157991DOI Listing
August 2022

The Anti-inflammation Property of Olfactory Ensheathing Cells in Neural Regeneration After Spinal Cord Injury.

Mol Neurobiol 2022 Aug 13. Epub 2022 Aug 13.

Translational Medicine Center, Hong Hui Hospital, Xi'an Jiaotong University, Xi'an, 710054, China.

Neural regeneration has troubled investigators worldwide in the past decades. Currently, cell transplantation emerged as a breakthrough targeted therapy for spinal cord injury (SCI) in the neurotrauma field, which provides a promising strategy in neural regeneration. Olfactory ensheathing cells (OECs), a specialized type of glial cells, is considered as the excellent candidate due to its unique variable and intrinsic regeneration-supportive properties. In fact, OECs could support olfactory receptor neuron turnover and axonal extension, which is essential to maintain the function of olfactory nervous system. Hitherto, an increasing number of literatures demonstrate that transplantation of OECs exerts vital roles in neural regeneration and functional recovery after neural injury, including central and peripheral nervous system. It is common knowledge that the deteriorating microenvironment (ischemia, hypoxia, scar, acute and chronic inflammation, etc.) resulting from injured nervous system is adverse for neural regeneration. Interestingly, recent studies indicated that OECs could promote neural repair through improvement of the disastrous microenvironments, especially to the overwhelmed inflammatory responses. Although OECs possess unusual advantages over other cells for neural repair, particularly in SCI, the mechanisms of OEC-mediated neural repair are still controversial with regard to anti-inflammation. Therefore, it is significant to summarize the anti-inflammation property of OECs, which is helpful to understand the biological characteristics of OECs and drive future studies. Here, we mainly focus on the anti-inflammatory role of OECs to make systematic review and discuss OEC-based therapy for CNS injury.
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http://dx.doi.org/10.1007/s12035-022-02983-4DOI Listing
August 2022

Characteristics of Living Systematic Review for COVID-19.

Clin Epidemiol 2022 4;14:925-935. Epub 2022 Aug 4.

Department of Pharmacy, West China Second University Hospital, Sichuan University, Chengdu, People's Republic of China.

Purpose: The systematic review aims to analyze and summarize the characteristics of living systematic review (LSR) for coronavirus disease 2019 (COVID-19).

Methods: Six databases including Medline, Excerpta Medica (Embase), Cochrane Library, China National Knowledge Infrastructure (CNKI), Wanfang Database and China Science, and Technology Journal Database (VIP), were searched as the source of basic information and methodology of LSR. Descriptive analytical methods were used to analyze the included COVID-19 LSRs, and the study characteristics of COVID-19 LSRs were further assessed.

Results: Sixty-four COVID-19 LSRs were included. Eighty-nine point one percent of LSRs were published on Science Citation Index (SCI) journals, and 64.1% publication with an impact factor (IF) >5 and 17.2% with an IF >15 among SCI journals. The first unit of the published LSRs for COVID-19 came from 19 countries, with the largest contribution from the UK (17.2%, 11/64). Forty point six percent of LSRs for COVID-19 were related to therapeutics topic which was considered the most concerned perspective for LSRs for COVID-19. Seventy-six point six percent of LSRs focused on the general population, with less attention to children, pregnant women and the elderly. However, the LSR for COVID-19 was reported incomplete on "living" process, including 40.6% of studies without search frequency, 79.7% of studies without screening frequency, 20.3% of studies without update frequency, and 65.6% of studies without the timing or criteria of transitioning LSR out of living mode.

Conclusion: Although researchers in many countries have applied LSRs to COVID-19, most of the LSRs for COVID-19 were incomplete in reporting on the "living" process and less focused on special populations. This could reduce the confidence of health-care providers and policy makers in the results of COVID-19 LSR, thereby hindering the translation of evidence on COVID-19 LSR into clinical practice. It was necessary to explicitly enact preferred reporting items for systematic reviews and meta-analyses (PRISMA) to improve the reporting quality of LSR and support ongoing efforts of therapeutics research for special patients with COVID-19.
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http://dx.doi.org/10.2147/CLEP.S367339DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9359410PMC
August 2022

The mechanism of RNA capping by SARS-CoV-2.

Nature 2022 Aug 9. Epub 2022 Aug 9.

Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA.

The SARS-CoV-2 RNA genome contains a 5'-cap that facilitates translation of viral proteins, protection from exonucleases and evasion of the host immune response. How this cap is made in SARS-CoV-2 is not completely understood. Here, we reconstitute the SARS-CoV-2 GpppA-RNA cap using virally encoded non-structural proteins (nsps). We show that the kinase-like NiRAN domain of nsp12 transfers RNA to the amino terminus of nsp9, forming a covalent RNA-protein intermediate (a process termed RNAylation). Subsequently, the NiRAN domain transfers RNA to GDP, forming the core cap structure GpppA-RNA. The nsp14 and nsp16 methyltransferases then add methyl groups to form functional cap structures. Structural analyses of the replication-transcription complex bound to nsp9 identified key interactions that mediate the capping reaction. Furthermore, we demonstrate in a reverse genetics system that the N-terminus of nsp9 and the kinase-like active site residues in the NiRAN domain are required for successful SARS-CoV-2 replication. Collectively, our results reveal an unconventional mechanism by which SARS-CoV-2 caps its RNA genome, thus exposing a new target in the development of antivirals to treat COVID-19.
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http://dx.doi.org/10.1038/s41586-022-05185-zDOI Listing
August 2022

Divergent kinase WNG1 is regulated by phosphorylation of an atypical activation sub-domain.

Biochem J 2022 Aug 8. Epub 2022 Aug 8.

The University of Texas Southwestern Medical Center, Dallas, Texas, United States.

Apicomplexan parasites like Toxoplasma gondii grow and replicate within a specialized organelle called the parasitophorous vacuole. The vacuole is decorated with parasite proteins that integrate into the membrane after trafficking through the parasite secretory system as soluble, chaperoned complexes. A regulator of this process is an atypical protein kinase called WNG1. Phosphorylation by WNG1 appears to serve as a switch for membrane integration. However, like its substrates, WNG1 is secreted from the parasite dense granules, and its activity must therefore be tightly regulated until the correct membrane is encountered. Here we demonstrate that, while another member of the WNG family can adopt multiple multimeric states, WNG1 is monomeric and therefore not regulated by multimerization. Instead, we identify two phosphosites on WNG1 that are required for its kinase activity. Using a combination of in vitro biochemistry and structural modeling, we identify basic residues that are also required for WNG1 activity and appear to recognize the activating phosphosites. Among these coordinating residues are the "HRD" Arg, which recognizes activation loop phosphorylation in canonical kinases. WNG1, however, is not phosphorylated on its activation loop, but rather on atypical phosphosites on its C-lobe. We propose a simple model in which WNG1 is activated by increasing ATP concentration above a critical threshold once the kinase traffics to the parasitophorous vacuole.
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http://dx.doi.org/10.1042/BCJ20220076DOI Listing
August 2022

Genome-wide identification of the pectin methylesterase inhibitor genes in and expression analysis of selected members.

Front Plant Sci 2022 22;13:940284. Epub 2022 Jul 22.

School of Life Sciences, Jiangsu University, Zhenjiang, China.

Pectin methylesterase inhibitors (PMEIs) modulate the status of pectin methylesterification by inhibiting the activity of pectin methylesterase (PME). Recent advances indicate PMEIs play an important role in regulating plant cell wall properties and defense responses. In this study, a genome-wide analysis of gene family in () was conducted and the expression patterns of genes in response to () was investigated. A total of 190 PMEI proteins were identified from the genome of . Chromosomal location, gene structure and properties of the PMEI family were analyzed, and these features were compared with . A total of 123 syntenic ortholog pairs were detected from family by synteny analysis. Results showed the expansion of genes was likely predominately from whole-genome duplication (WGD) or segmental duplications. Multiple -elements related to plant growth and development, environmental stress responses, hormone responses were detected in the promoters of genes, implying they were regulated by both internal and external factors. Furthermore, expression analysis of transcriptome data combined with quantitative RT-PCR (qRT-PCR) validation identified several candidates that were strongly responsive to infection. These genes are candidates for manipulation to breed novel and improved genotypes that are more resistant to sclerotinia stem rot (SSR). Extensive interactions were detected among 30 BnPMEI proteins, forming complex protein-protein interaction networks. Besides, 48 BnPMEIs showed interactions with other proteins including a range of cell wall structure-related enzymes. This study provides new insights into the evolution and function of PMEIs in and lays a foundation for breeding novel genotypes for crop improvement.
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http://dx.doi.org/10.3389/fpls.2022.940284DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354821PMC
July 2022

Traditional Chinese Medicine Injections Combined With Oseltamivir for Influenza: Systematic Review and Network Meta-Analysis.

Front Pharmacol 2022 22;13:848770. Epub 2022 Jul 22.

First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, China.

As a cause of respiratory tract infections in humans, influenza remains with high morbidity and mortality, with associated significant healthcare burden and increased financial burden. Traditional Chinese medicine injections (TCMIs) combined with oseltamivir (TCMIs + oseltamivir) are the representative therapeutic strategies for influenza, which is a compliant with clinical applications in China. The aim of this study was to describe the comparative efficacy and safety of TCMIs + oseltamivir in patients with influenza, based on the current evidence. PubMed, Embase, Cochrane Library, Web of Science, China National Knowledge Infrastructure, Wanfang Data Knowledge Service Platform, VIP information resource integration service platform databases, and the Chinese biomedical literature service system were searched to find randomized controlled trials where TCMIs + oseltamivir are the representative therapeutic strategies for influenza, from inception until October 2021, without language restriction. Two investigators independently screened eligibility criteria, extracted data, and appraised the risk of bias with the same criteria. We conducted a network meta-analysis using the Bayesian random method for each outcome and performed the sensitivity analysis, meta-regression, and Egger's and Begg's tests for the reliability and robustness of our results. Thirty-one trials including 2,893 participants proved eligible and reported on four TCMIs + oseltamivir oseltamivir. Network meta-analysis showed Yanhuning (YHN) +oseltamivir (MD = -1.7, 95% CrI: -2.5 to -0.88; SUCRA = 0.89; low certainty of evidence) in fever disappearance time, Tanreqing (TRQ) +oseltamivir (MD = -1.9, 95% CrI: -2.8 to -1; SUCRA = 0.97; low certainty of evidence) in cough disappearance time, and Xiyanping (XYP) +oseltamivir (OR = 5.9, 95% CrI: 3.1 to 11; SUCRA = 0.82; very low certainty of evidence) in the response rate to be more efficacious than oseltamivir alone with the best SUCRA. Based on the combined SUCRA value for primary outcomes, TRQ + oseltamivir is probably better in cough disappearance time, and XYP + oseltamivir and YHN + oseltamivir may be better in fever disappearance time than others. No significant difference in safety between the treatments. In patients with influenza, TCMIs + oseltamivir only partially improve flu symptoms. Overall therapeutic efficacy and safety are inconclusive, based on low to very low certainty of evidence. However, the safety remains uncertain, and TCMI treatments for influenza should be considered with caution. More high-quality studies examining the efficacy and safety of TCMIs are needed. https://www.crd.york.ac.uk/PROSPERO/, identifier CRD42021286994.
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http://dx.doi.org/10.3389/fphar.2022.848770DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355026PMC
July 2022

Microplastics contamination in eggs: Detection, occurrence and status.

Food Chem 2022 Jul 25;397:133771. Epub 2022 Jul 25.

State Key Laboratory of Food Science and Technology, Jiangnan University, No. 1800 Lihu Avenue, Wuxi 214122, Jiangsu Province, China; School of Food Science and Technology, Jiangnan University, No. 1800 Lihu Avenue, Wuxi 214122, Jiangsu Province, China; Joint International Research Laboratory of Food Safety, Jiangnan University, No. 1800 Lihu Avenue, Wuxi 214122, Jiangsu Province, China. Electronic address:

Nowadays, the widespread distribution of microplastics (MPs) in various foods has received much attention. In this study, eggs were purchased as samples from local supermarkets to detect and analyze the presence of MPs. The digestion method was optimized for eggs to better isolate MPs, which proved MPs indeed exist in eggs, and the average content was 11.67 ± 3.98 particles/egg. The shape and size range of most MPs were spherical and 50-100 μm. The infrared results showed that the main type of MPs in eggs was polyethylene. The number of MPs in egg yolk was higher than that in egg white, and there was no significant change after cooking. In addition, the daily human intake of MPs was estimated. Therefore, the results of this study provided a background for the current status and pollution of MPs in eggs, and proposed the necessary control and preventive measures to avoid this situation.
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http://dx.doi.org/10.1016/j.foodchem.2022.133771DOI Listing
July 2022

Neuroprotective Effects and Mechanisms of Senegenin, an Effective Compound Originated From the Roots of Polygala Tenuifolia.

Front Pharmacol 2022 18;13:937333. Epub 2022 Jul 18.

National Chinmedomics Research Center, National TCM Key Laboratory of Serum Pharmacochemistry, Metabolomics Laboratory, Department of Pharmaceutical Analysis, Heilongjiang University of Chinese Medicine, Harbin, China.

Senegenin is the main bioactive ingredient isolated from the dried roots of Willd. In recent years, senegenin has been proved to possess a variety of pharmacological activities, such as anti-oxidation, anti-inflammation, anti-apoptosis, enhancement of cognitive function. Besides, it has a good development prospect for the treatment of neurodegenerative diseases, depression, osteoporosis, cognitive dysfunction, ischemia-reperfusion injury and other diseases. However, there is no systematic literature that fully demonstrates the pharmacological effects of senegenin. In order to meet the needs of new drug research and precise medication, this review summarized the neuroprotective effects, mechanisms and gastrointestinal toxicity of senegenin based on the literatures published from the past 2 decades. In addition, an in-depth analysis of the existing problems in the current research as well as the future research directions have been conducted in order to provide a basis for the clinical application of this important plant extract.
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http://dx.doi.org/10.3389/fphar.2022.937333DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9341472PMC
July 2022

A customized early warning score enhanced emergency department patient flow process and clinical outcomes in a COVID-19 pandemic.

J Am Coll Emerg Physicians Open 2022 Aug 30;3(4):e12783. Epub 2022 Jul 30.

Lehigh Valley Health Network Department of Emergency and Hospital Medicine/USF Morsani College of Medicine Bethlehem Pennsylvania USA.

Objective: Patient crowding and boarding in the emergency department (ED) is associated with adverse outcomes and has become increasingly problematic in recent years. We investigated the impact of an ED patient flow countermeasure using an early warning score.

Methods: We conducted a cross-sectional analysis of observational data from patients who presented to the ED of a Level 1 Trauma Center in Pennsylvania. We implemented a modified version of the Modified Early Warning Score (MEWS), called mMEWS, to address patient flow. Patients aged ≥18 years old admitted to the adult hospital medicine service were included in the study. We compared the pre-mMEWS (February 19, 2017-February 18, 2019) to the post-mMEWS implementation period (February 19, 2019-June 30, 2020). During the intervention, low MEWS (0-1) scoring admissions went directly to the inpatient floor with expedited orders, the remainder waited in the ED until the hospital medicine admitting team evaluated the patient and then placed orders. We investigated the association between mMEWS, ED length of stay (LOS), and 24-hour rapid response team (24 hour-RRT) activation. RRT activation rates were used as a measure of adverse outcome for the new process and are a network team response for admitted patients who are rapidly decompensating. The association between mMEWS and the outcomes of ED length of stay in minutes and 24 hour-RRT activation was assessed using linear and logistic regression adjusting for a priori selected confounders, respectively.

Results: Of the total 43,892 patients admitted, 19,962 (45.5%) were in the pre-mMEWS and 23,930 (54.5%) in the post-mMEWS implementation period. The median post-mMEWS ED LOS was shorter than the pre-mMEWS (376 vs 415 minutes;  < 0.01). After accounting for potential confounders, there was a 4.57% decrease in the ED LOS after implementing mMEWS (95% confidence interval [CI], 4.20-4.94;  < 0.01). The proportion of 24 hour-RRT did not differ significantly when comparing pre- and post-mMEWS (33.5% vs 34.4%;  = 0.83).

Conclusion: The use of a modified MEWS enhanced admission process to the hospital medicine service, even during the COVID-19 pandemic, was associated with a significant decrease in ED LOS without a significant increase in 24 hour-RRT activation.
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http://dx.doi.org/10.1002/emp2.12783DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338822PMC
August 2022

Are Grid-Like Representations a Component of All Perception and Cognition?

Front Neural Circuits 2022 14;16:924016. Epub 2022 Jul 14.

Department of Neurosurgery, Xinqiao Hospital, Army Medical University, Chongqing, China.

Grid cells or grid-like responses have been reported in the rodent, bat and human brains during various spatial and non-spatial tasks. However, the functions of grid-like representations beyond the classical hippocampal formation remain elusive. Based on accumulating evidence from recent rodent recordings and human fMRI data, we make speculative accounts regarding the mechanisms and functional significance of the sensory cortical grid cells and further make theory-driven predictions. We argue and reason the rationale why grid responses may be universal in the brain for a wide range of perceptual and cognitive tasks that involve locomotion and mental navigation. Computational modeling may provide an alternative and complementary means to investigate the grid code or grid-like map. We hope that the new discussion will lead to experimentally testable hypotheses and drive future experimental data collection.
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http://dx.doi.org/10.3389/fncir.2022.924016DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9329517PMC
August 2022

Monocyte to High-Density lipoprotein and Apolipoprotein A1 Ratios: Novel Indicators for Metabolic Syndrome in Chinese Newly Diagnosed Type 2 Diabetes.

Front Endocrinol (Lausanne) 2022 14;13:935776. Epub 2022 Jul 14.

Longyan First Affiliated Hospital of Fujian Medical University, Longyan, China.

Objective: Increasing evidence highlighted that chronic inflammation involved in the development of metabolic syndrome (MetS) and Type 2 diabetes mellitus (T2DM). This prospective study was aimed to assess the association between MetS and novel pro-inflammatory indicators like monocyte-to-high-density lipoprotein and monocyte-to-apolipoprotein A1 ratios (MHR and MAR) in Chinese newly diagnosed T2DM.

Method: A total of 605 Chinese newly diagnosed T2DM with complete and available data were enrolled in this study. Demographic and anthropometric information were collected. Laboratory assessments were determined by standard methods. MetS was based on the Chinese Diabetes Society definition. Multiple binomial logistic regression model was used to estimate the independent variables of MHR and MAR for MetS. Receiver operating characteristic (ROC) curve was conducted to assess the optimal cutoff value of MHR and MAR in identifying MetS.

Results: Overall, the prevalence of MetS was 60.2%. The correlation analysis showed that MHR and MAR were closely correlated with metabolic risk factors like body mass index, waist circumference, triglycerides, high-density lipoprotein cholesterol, systolic blood pressure, diastolic blood pressure, uric acid, and insulin resistance. MHR and MAR were also significantly associated with higher odds of MetS after adjustment for other confounders, the odds ratios (ORs) (95%CI) were 1.50 (1.14-1.97) and 2.26(1.79-2.87) respectively. Furthermore, MHR and MAR were also seemed to have higher area under the curve (AUC) for MetS than ApoA1 and monocyte alone from the ROC curve analysis ( < 0.05). The AUCs of MHR and MAR identifying MetS were 0.804 (95% CI: 0.768-0.839) and 0.840 (95% CI: 0.806-0.873) respectively ( < 0.001). The optimal cutoff values of MHR and MAR were 3.57 × 10/mmol (sensitivity: 76.1%, specificity: 73.4%) and 3.95 × 10/g (sensitivity: 79.7%, specificity: 84.6%), respectively.

Conclusions: MHR and MAR were significantly associated with MetS. These two novel pro-inflammatory indicators may be useful markers for MetS in Chinese newly diagnosed T2DM.
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http://dx.doi.org/10.3389/fendo.2022.935776DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330493PMC
August 2022

The Application of Nimotuzumab Combined With Definitive Chemoradiotherapy Toward the Treatment of Locally Advanced Cervical Esophageal Carcinoma: A Retrospective Study.

Front Oncol 2022 6;12:905422. Epub 2022 Jul 6.

Department of Radiotherapy and Chemotherapy, Hwamei Hospital, University of Chinese Academy of Sciences, Ningbo, China.

Objective: To evaluate the safety and effectiveness of nimotuzumab in combination with chemoradiotherapy for locally advanced cervical esophageal squamous cell carcinoma.

Methods: Retrospective analysis was conducted from September 2012 to February 2017 among 50 locoregional-advanced cervical esophageal carcinoma (CEC) patients who received concurrent chemoradiotherapy (CRT) combined with or without nimotuzumab at Ningbo Medical Center Lihuili Hospital. Intensity-modulated radiotherapy (IMRT) was administrated on all patients. All patients were divided into two groups, of which 26 (Group A) received 200 mg (22 of 50) or 400 mg (4 of 50) of nimotuzumab per week with CRT and 24 (Group B) received definitive CRT.

Results: The median follow-up time was 23 months. The median overall survival (OS) and progression-free survival (PFS) were 40.6 and 21.1 months for all, respectively. The 1-, 2-, and 3-year OS rates on the whole were 79.6%, 62.1%, and 47.8%. There was no statistical difference in overall response rate and disease control rate between the two groups. Patients treated with nimotuzumab (group A) had better PFS than the definitive CRT group (group B) ( < 0.05). However, the median OS was 41.4 months in group A and 32.4 months in group B, respectively ( = 0.517). Multivariate analysis showed that PFS among those with lower Eastern Cooperative Oncology Group (ECOG) score (HR = 5.11; < 0.01), stage II (HR = 9.52; < 0.01) and the application of nimotuzumab combined with CRT (HR = 0.16; < 0.01) was much longer. Furthermore, ECOG, stage, C-reactive protein (CRP) baseline, and histological grade can also be used as independent predictors of OS. Grade >3 adverse reactions were not observed. The most common adverse event related to nimotuzumab was mild fever and the occurrence rate was 19% (5 of 26). The incidence of anemia was 65.4% in group A and 87.5% in group B ( < 0.05).

Conclusions: For locoregional-advanced CEC, nimotuzumab combined with IMRT and concomitant chemotherapy was tolerated and effective. In addition, patients with a normal pretherapeutic serum CRP level (CRP < 10 mg/L) can achieve better OS.
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http://dx.doi.org/10.3389/fonc.2022.905422DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9310542PMC
July 2022

Ciliogenesis requires sphingolipid-dependent membrane and axoneme interaction.

Proc Natl Acad Sci U S A 2022 Aug 27;119(31):e2201096119. Epub 2022 Jul 27.

Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing 100084, China.

Cilium formation and regeneration requires new protein synthesis, but the underlying cytosolic translational reprogramming remains largely unknown. Using ribosome footprinting, we performed global translatome profiling during cilia regeneration in and uncovered that flagellar genes undergo an early transcriptional activation but late translational repression. This pattern guided our identification of sphingolipid metabolism enzymes, including serine palmitoyltransferase (SPT), as essential regulators for ciliogenesis. Cryo-electron tomography showed that ceramide loss abnormally increased the membrane-axoneme distance and generated bulged cilia. We found that ceramides interact with intraflagellar transport (IFT) particle proteins that IFT motors transport along axoneme microtubules (MTs), suggesting that ceramide-IFT particle-IFT motor-MT interactions connect the ciliary membrane with the axoneme to form rod-shaped cilia. SPT-deficient vertebrate cells were defective in ciliogenesis, and SPT mutations from patients with hereditary sensory neuropathy disrupted cilia, which could be restored by sphingolipid supplementation. These results reveal a conserved role of sphingolipid in cilium formation and link compromised sphingolipid production with ciliopathies.
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http://dx.doi.org/10.1073/pnas.2201096119DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9351462PMC
August 2022

Visualizing MiRNA Regulation of Apoptosis for Investigating the Feasibility of MiRNA-Targeted Therapy Using a Fluorescent Nanoprobe.

Pharmaceutics 2022 Jun 25;14(7). Epub 2022 Jun 25.

Shandong Provincial Key Laboratory of Molecular Engineering, School of Chemistry and Chemical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, China.

MiRNA-targeted therapy is an active research field in precision cancer therapy. Studying the effect of miRNA expression changes on apoptosis is important for evaluating miRNA-targeted therapy and realizing personalized precision therapy for cancer patients. Here, a new fluorescent nanoprobe was designed for the simultaneous imaging of miRNA-21 and apoptotic protein caspase-3 in cancer cells by using gold nanoparticles as the core and polydopamine as the shell. Confocal imaging indicated that the nanoprobe could be successfully applied for in situ monitoring of miRNA regulation of apoptosis. This design strategy is critical for investigating the feasibility of miRNA-targeted therapy, screening new anti-cancer drugs targeting miRNA, and developing personalized treatment plans.
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http://dx.doi.org/10.3390/pharmaceutics14071349DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9323288PMC
June 2022

Simultaneous Visualization of MiRNA-221 and Caspase-3 in Cancer Cells for Investigating the Feasibility of MiRNA-Targeted Therapy with a Dual-Color Fluorescent Nanosensor.

Biosensors (Basel) 2022 Jun 23;12(7). Epub 2022 Jun 23.

Shandong Provincial Key Laboratory of Molecular Engineering, School of Chemistry and Chemical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, China.

MiRNA-targeted therapy holds great promise for precision cancer therapy. It is important to investigate the effect of changes in miRNA expression on apoptosis in order to evaluate miRNA-targeted therapy and achieve personalized therapy. In this study, we designed a dual-color fluorescent nanosensor consisting of grapheme oxide modified with a molecular beacon and peptide. The nanosensor can simultaneously detect and image miRNA-221 and apoptotic protein caspase-3 in living cells. Intracellular experiments showed that the nanosensor could be successfully applied for in situ monitoring of the effect of miRNA-221 expression changes on apoptosis by dual-color imaging. The current strategy could provide new avenues for investigating the feasibility of miRNA-targeted therapy, screening new anti-cancer drugs targeting miRNA and developing personalized treatment plans.
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http://dx.doi.org/10.3390/bios12070444DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9312853PMC
June 2022

Improving soil aggregates stability and soil organic carbon sequestration by no-till and legume-based crop rotations in the North China Plain.

Sci Total Environ 2022 Jul 22;847:157518. Epub 2022 Jul 22.

College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China; Innovation Center of Agricultural Technology for Lowland Plain of Hebei, Wuqiao, China.

Conservation agriculture (CA) has been adopted worldwide on about 200 Mha to enhance soil organic carbon (SOC) for mitigating climate change. However, as a crucial mechanism to sequester SOC, how the protection of aggregates responds to the interaction between no-till and crop rotations (two principles of CA) remains unknown. Thus, a field experiment with six treatments [e.g., no-till or rotary tillage under the maize-wheat-soybean-wheat system (NT-MWSW, RT-MWSW), no-till or rotary tillage under the maize-wheat system (NT-MW, RT-MW), and no-till or rotary tillage under the soybean-wheat system (NT-SW, RT-SW)] was conducted from June 2018 to June 2021 in the North China Plain (NCP) to assess their effects on aggregation and SOC. Results indicated that macroaggregates (> 0.25 mm) were the main contributors to the soil carbon (C) pool, comprised 64.7-87.3 % of aggregates, and encompassed 64.9-73.1 % of the SOC stock. NT increased not only the proportion of macroaggregates but also aggregate stability (i.e., mean weight diameter and geometric mean diameter). Significant positive effects from legumes were observed under NT. SW increased by 13.6 % macroaggregate-associated SOC under NT in 0-20 cm compared to that under MW. Additionally, the conversion rate of straw C input under NT-SW was higher than that in other treatments, augmenting it by 9.4-21.9 %. This may be attributed to the higher macroaggregate total nitrogen (increased by 1.7-15.9 %) in 0-10 cm under legume-based crop rotations compared to that under MW, resulting in lower C: N ratios, which promoted the decomposition of straw. Furthermore, the total potential mineralization of macroaggregates under NT legume-based crop rotations was 3.0-16.0 % higher than that of MW. Thus, a legume-based NT system can significantly improve soil macro-aggregation, increase the conversion rate of straw C input, and reduce C loss, which can be a viable practice to enhance SOC sequestration capacity under CA in the NCP.
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http://dx.doi.org/10.1016/j.scitotenv.2022.157518DOI Listing
July 2022

Rapid characterization of chemical constituents in Naoling Pian by LC-MS combined with data processing techniques.

J Sep Sci 2022 Jul 20. Epub 2022 Jul 20.

National Chinmedomics Research Center, National TCM Key Laboratory of Serum Pharmacochemistry, Functional Metabolomics Laboratory, Department of Pharmaceutical Analysis, Heilongjiang University of Chinese Medicine, Heping Road 24, Harbin, China.

Naoling Pian is a prescription composed of 15 herbs, which is mainly used for the treatment of insomnia in clinical practice. However, the chemical constituents in Naoling Pian are numerous and unclear, which hinders the interpretation of its bioactive constituents and the subsequent research on the material basis for pharmacodynamics. The purpose of this study is to develop a rapid method for identifying the chemical constituents of Naoling Pian using high-throughput ultra-performance liquid chromatography quadrupole time of flight coupled with mass spectrometry combined with a software platform for data processing. The whole composition of Naoling Pian was characterized in positive and negative ion modes. In this experiment, an overall total of 201 constituents were identified by using reference standards, online and self-built databases matching, fragmentation rules analysis of mass spectrometry peaks with a software platform. Meanwhile, Naoling Pian was analyzed for the first time using LC-MS method, the constituents could be identified in a quick and accurate manner, and the results could provide a scientific basis for the follow-up research on the pharmacodynamic material basis and quality control of Naoling Pian. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/jssc.202200244DOI Listing
July 2022

Modulation of the Gut Microbiota by Fufang-Zhenzhu-Tiaozhi Capsule Attenuates Hypertension Induced by a High-Fructose and High-Salt Diet.

Front Cell Infect Microbiol 2022 29;12:854849. Epub 2022 Jun 29.

Guangdong Metabolic Disease Research Center of Integrated Chinese and Western Medicine, Guangdong Pharmaceutical University, Guangzhou, China.

Hypertension is frequently comorbid with the disorders of glucose and lipid metabolism. The increased intakes of fructose and salt contribute to the development of hypertension and related metabolic disorders, which are closely associated with gut dysbiosis. Fufang-Zhenzhu-Tiaozhi capsule (FTZ), a traditional Chinese patent medicine commonly used in clinical practice, has recently emerged as a promising drug candidate for metabolic diseases. In this study, FTZ treatment is identified as attenuating blood pressure increase and improving the metabolism of lipid and uric acid in high-fructose and high-salt (HFS) diet-fed rats. FTZ also substantially alleviated renal fibrosis and the mRNA expression of inflammation cytokines, NADPH oxidases, and the renin-angiotensin system in the renal cortex. 16S rRNA sequencing of fecal samples revealed that FTZ restored HFS-induced gut dysbiosis, seen as increased intestinal microbial richness and diversity. Furthermore, fecal microbiota transplantation also achieved similar therapeutic effects and alterations in gut microbiota profile induced by FTZ. Taken together, this study highlights the efficacy of FTZ in attenuating HFS-induced hypertension and related metabolic disorders and renal injury. The antihypertensive effect is associated with the modulation of gut microbiota.
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http://dx.doi.org/10.3389/fcimb.2022.854849DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9277139PMC
June 2022

Exploring the potential targets of Sanshimao formula for hepatocellular carcinoma treatment by a method of network pharmacology combined with molecular biology.

J Ethnopharmacol 2022 Oct 15;297:115531. Epub 2022 Jul 15.

Department of Traditional Chinese Medicine, the First Affiliated Hospital of Naval Military Medical University, Shanghai, China. Electronic address:

Ethnopharmacological Relevance: The Sanshimao (SSM) formula is an effective prescription for hepatocellular carcinoma (HCC) therapy in the clinical setting. This prescription is made up of four herbals, Maorenshen, Shijianchuan, Shishangbai and Shidachuan, which are used for detoxification and removing blood stasis. However, its mechanism in the treatment of HCC remains ambiguous.

Aim Of The Study: To explore the potential targets of SSM against HCC by network pharmacology analysis and verify the data using molecular biological methods.

Materials And Methods: We screened active components and potential targets by data mining, constructed a network, and performed functional analysis and pathway enrichment to explore the therapeutic targets of SSM for HCC treatment. Then, the effects of SSM on HCC cells were studied to validate the data from network pharmacology analysis.

Results: Eighty-eight common targets were obtained by mapping 932 HCC-related genes, and 325 targets corresponded to 11 active components of SSM. They were enriched in various biological processes, such as the response to inorganic substances, response to toxic substances and apoptotic signalling pathway, and multi-pathways involved pathways in cancer, EGFR tyrosine kinase inhibitor resistance, and AGE-RAGE signalling pathway in diabetic complications, as evaluated by the analysis of advanced functions and pathways. TP53, JUN, HSP90AA1, EGFR, AR and MAPK1 might be the core targets closely related to the effects of SSM on HCC according to PPI analysis. Treatment with SSM decreased cell viability and migration, promoted apoptosis and inhibited the EGFR/FAK/AKT signalling pathway.

Conclusion: This research preliminarily indicates that SSM treats HCC via multiple components and pathways. EGFR/FAK/AKT are promising therapeutic targets of SSM for HCC treatment. This provides objective evidence for further mechanistic research and the future development and clinical application of SSM in HCC patients.
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http://dx.doi.org/10.1016/j.jep.2022.115531DOI Listing
October 2022

Whole-cell FRET monitoring of transcription factor activities enables functional annotation of signal transduction systems in living bacteria.

J Biol Chem 2022 Jul 12:102258. Epub 2022 Jul 12.

School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong SAR, China. Electronic address:

Bacteria adapt to their constantly changing environments largely by transcriptional regulation through the activities of various transcription factors (TFs). However, techniques that monitor TF-promoter interactions in situ in living bacteria are lacking. Herein, we developed a whole-cell TF-promoter binding assay based on the intermolecular Förster resonance energy transfer (FRET) between an unnatural amino acid, L-(7-hydroxycoumarin-4-yl) ethylglycine (CouA), which labels TFs with bright fluorescence through genetic encoding (donor fluorophore) and the live cell nucleic acid stain SYTO 9 (acceptor fluorophore). We show that this new FRET pair monitors the intricate TF-promoter interactions elicited by various types of signal transduction systems, including one-component (CueR) and two-component systems (BasSR and PhoPQ), in bacteria with high specificity and sensitivity. We demonstrate that robust CouA incorporation and FRET occurrence is achieved in all regulatory systems tested based on either the crystal structures of TFs or their simulated structures, if 3D structures of the TFs were unavailable. Furthermore, using CueR and PhoPQ systems as models, we demonstrate that the whole-cell FRET assay is applicable for the identification and validation of complex regulatory circuit and novel modulators of regulatory systems of interest. Lastly, we show that the FRET system is applicable for single-cell analysis and for monitoring TF activities in E. coli colonizing a C. elegans host. In conclusion, we established a tractable and sensitive TF-promoter binding assay which not only complements currently available approaches for DNA-protein interactions, but also provides novel opportunities for functional annotation of bacterial signal transduction systems and studies of the bacteria-host interface.
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http://dx.doi.org/10.1016/j.jbc.2022.102258DOI Listing
July 2022

The role of processing efficiency and selection history in the limit of visual awareness in shape perception.

J Vis 2022 07;22(8)

School of Psychology, Speech, and Hearing, University of Canterbury, Christchurch, New Zealand.

A growing body of research indicates that the limit in instant conscious awareness, or the unit of access, for some object features such as color, orientation, and direction of motion is more than one. In four experiments we explored the roles of processing efficiency and selection history in shape perception. Two targets, which were geometric shapes (less efficient) or alphabet letters (more efficient), were shown simultaneously or sequentially. The task was to judge whether a test probe matched one of the targets. In different experiments, the two types of trials were presented in separate blocks, interleaved couplets, or randomly within the same block during testing but regrouped in data analyses such that the same type of trials was either repeated or not repeated. Accuracy was higher in the sequential than simultaneous trials for geometric shapes, but not for upright letters, when the same trial type was blocked or in the repeated condition. These results suggest that processing efficiency and selection history both play a role in the unit of access in shape perception. They also underscore the flexibility of the visual system, which uses different factors to maximize conscious visual perception at a given time.
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http://dx.doi.org/10.1167/jov.22.8.9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9296887PMC
July 2022

Author Correction: Steering surface reconstruction of copper with electrolyte additives for CO electroreduction.

Nat Commun 2022 Jul 13;13(1):4059. Epub 2022 Jul 13.

Nanoyang Group, State Key Laboratory of Chemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.

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http://dx.doi.org/10.1038/s41467-022-31696-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9279470PMC
July 2022

A clamp-free micro-stretching system for evaluating the viscoelastic response of cell-laden microfibers.

Biosens Bioelectron 2022 Oct 3;214:114517. Epub 2022 Jul 3.

Key Laboratory of Biomimetic Robots and Systems (Beijing Institute of Technology), Ministry of Education, 100081, People's Republic of China; Beijing Advanced Innovation Center for Intelligent Robots and Systems, Beijing Institute of Technology, 100081, People's Republic of China; School of Mechatronical Engineering, Beijing Institute of Technology, Beijing, 100081, People's Republic of China. Electronic address:

Viscoelastic hydrogel microfibers have extensive applications in tissue engineering and regenerative medicine, however, their viscoelasticity is still difficult to be directly characterized because microfiber-specific measuring system is lacking for quantitative studies. In this paper, we develop a two-probe micro-stretching system to quantitatively investigate viscoelasticity of the microfiber by evaluating the storage and loss modulus: E' and E″. A liquid bridge-based fixation method enables single microfiber to be easily fixed to be stably stretched by a two-probe actuator. Afterward, multi-frequency stretching force loading is automatically implemented based on real-time force control, and the resulting stress and strain in the frequency spectrum are measured to evaluate the E' and E″ of pure GelMA, alginate-GelMA composite and GelMA core-alginate shell microfibers. The measured E' and E″ are verified by the response of NIH/3T3 fibroblast cells to the composite microfibers with different alginate concentrations. Moreover, benefiting from the low-damaged stretching process, our system can also detect the difference of the E' and E″ between two cellular processes including growth and differentiation of the aligned mesenchymal stem cells in the same one core-shell microfiber. These results all show that our proposed system provides a valuable reference tool for biomaterials design, the study of cell-matrix interaction and disease etiology from the perspective of mechanics.
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http://dx.doi.org/10.1016/j.bios.2022.114517DOI Listing
October 2022

A polyaniline surface-modified Prussian blue analogue cathode for flexible aqueous Zn-ion batteries.

Chem Commun (Camb) 2022 Jul 21;58(59):8226-8229. Epub 2022 Jul 21.

Sauvage Laboratory for Smart Materials, School of Materials Science and Engineering, Harbin Institute of Technology, Shenzhen, 518055, China.

Aqueous Zn-ion batteries have gained popularity due to their low cost and high safety, but their low energy density limits their application scenarios. Although the Prussian blue analogue (PBA) has the characteristics of high redox potential, the poor capacity and cycling performance restrict its further development. Here, we propose a modification strategy of a polyaniline (PANI) coating on zinc ferricyanide (ZnHCF). The PANI coating inhibits the dissolution of ZnHCF and enables the Zn-ion battery to present two long-flat discharge voltage platforms as well as a high capacity of 150 mA h g, which provides a new idea for the development of high-performance PBA battery materials. Meanwhile, owing to the spring-like structure, the battery has a high stretchability of 600% and maintains stable electrochemical properties during stretching.
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http://dx.doi.org/10.1039/d2cc02724kDOI Listing
July 2022
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