Zhaohui Ye

Zhaohui Ye

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Zhaohui Ye

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Human-relevant preclinical in vitro models for studying hepatobiliary development and liver diseases using induced pluripotent stem cells.

Exp Biol Med (Maywood) 2019 May 26;244(8):702-708. Epub 2019 Feb 26.

1 Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center, Baltimore, MD 21205, USA.

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http://dx.doi.org/10.1177/1535370219834895DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6552401PMC
May 2019

Transient c-Src Suppression During Endodermal Commitment of Human Induced Pluripotent Stem Cells Results in Abnormal Profibrotic Cholangiocyte-Like Cells.

Stem Cells 2019 03 17;37(3):306-317. Epub 2018 Dec 17.

Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.

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http://dx.doi.org/10.1002/stem.2950DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6435276PMC
March 2019

Derivation of a disease-specific human induced pluripotent stem cell line from a biliary atresia patient.

Stem Cell Res 2017 10 8;24:25-28. Epub 2017 Aug 8.

Department of Oncology, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, USA; Cellular and Molecular Medicine Graduate Program, Johns Hopkins University School of Medicine, USA; Institute for Cell Engineering, Johns Hopkins University School of Medicine, USA. Electronic address:

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http://dx.doi.org/10.1016/j.scr.2017.08.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5881114PMC
October 2017

Generation of human iPSCs from an essential thrombocythemia patient carrying a V501L mutation in the MPL gene.

Stem Cell Res 2017 01 11;18:57-59. Epub 2016 Dec 11.

Division of Hematology, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Stem Cell Program, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.

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http://dx.doi.org/10.1016/j.scr.2016.12.012DOI Listing
January 2017

Gene correction in patient-specific iPSCs for therapy development and disease modeling.

Hum Genet 2016 09 2;135(9):1041-58. Epub 2016 Jun 2.

Department of Medicine, Johns Hopkins University School of Medicine, Ross Research Building, Room 1032, 720 Rutland Ave., Baltimore, 21205, MD, USA.

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http://dx.doi.org/10.1007/s00439-016-1691-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5881117PMC
September 2016

Efficient and Controlled Generation of 2D and 3D Bile Duct Tissue from Human Pluripotent Stem Cell-Derived Spheroids.

Stem Cell Rev Rep 2016 Aug;12(4):500-8

Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA.

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http://dx.doi.org/10.1007/s12015-016-9657-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6186008PMC
August 2016

A Method for Genome Editing in Human Pluripotent Stem Cells.

Cold Spring Harb Protoc 2016 Apr 1;2016(4):pdb.prot090217. Epub 2016 Apr 1.

Division of Hematology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205; Stem Cell Program, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205; Predoctoral Training Program in Human Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.

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http://dx.doi.org/10.1101/pdb.prot090217DOI Listing
April 2016

Genome Editing in Human Pluripotent Stem Cells.

Cold Spring Harb Protoc 2016 Apr 1;2016(4):pdb.top086819. Epub 2016 Apr 1.

Division of Hematology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205; Stem Cell Program, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205; Predoctoral Training Program in Human Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.

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http://dx.doi.org/10.1101/pdb.top086819DOI Listing
April 2016

Genome editing systems in novel therapies.

Discov Med 2016 Jan;21(113):57-64

Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.

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January 2016

A facile method to establish human induced pluripotent stem cells from adult blood cells under feeder-free and xeno-free culture conditions: a clinically compliant approach.

Stem Cells Transl Med 2015 Apr 5;4(4):320-32. Epub 2015 Mar 5.

Division of Hematology, Department of Medicine, and Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA; State Key Laboratory of Experimental Hematology, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences, and Department of Stem Cells and Regenerative Medicine, Peking Union Medical College, Tianjin, People's Republic of China; Department of Transfusion, Changhai Hospital, Second Military Medical University, Shanghai, People's Republic of China; Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, Maryland, USA; Key Laboratory of Pediatric Hematology/Oncology of Ministry of Health, Pediatric Translational Medicine Institute, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China

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http://dx.doi.org/10.5966/sctm.2014-0214DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4367508PMC
April 2015

Efficient and allele-specific genome editing of disease loci in human iPSCs.

Mol Ther 2015 Mar 24;23(3):570-7. Epub 2014 Nov 24.

1] Division of Hematology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA [2] Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.

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http://dx.doi.org/10.1038/mt.2014.226DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4351458PMC
March 2015

Covalent modification of a cysteine residue in the XPB subunit of the general transcription factor TFIIH through single epoxide cleavage of the transcription inhibitor triptolide.

Angew Chem Int Ed Engl 2015 Feb 12;54(6):1859-63. Epub 2014 Dec 12.

Department of Pharmacology, Johns Hopkins School of Medicine, 725 North Wolfe Street, Hunterian Building, Room 516, Baltimore, MD 21205 (USA).

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http://dx.doi.org/10.1002/anie.201408817DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4314353PMC
February 2015

Effectiveness of exome and genome sequencing guided by acuity of illness for diagnosis of neurodevelopmental disorders.

Sci Transl Med 2014 Dec;6(265):265ra168

Center for Pediatric Genomic Medicine, Children's Mercy-Kansas City, Kansas City, MO 64108, USA. Department of Pediatrics, Children's Mercy-Kansas City, Kansas City, MO 64108, USA. School of Medicine, University of Missouri-Kansas City, Kansas City, MO 64108, USA. Department of Pathology, Children's Mercy-Kansas City, Kansas City, MO 64108, USA.

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http://dx.doi.org/10.1126/scitranslmed.3010076DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4286868PMC
December 2014

Whole-genome sequencing analysis reveals high specificity of CRISPR/Cas9 and TALEN-based genome editing in human iPSCs.

Cell Stem Cell 2014 Jul;15(1):12-3

Division of Hematology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Stem Cell Program, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA. Electronic address:

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http://dx.doi.org/10.1016/j.stem.2014.06.011DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4338993PMC
July 2014

Roles of reactive oxygen species in the fate of stem cells.

Antioxid Redox Signal 2014 Apr 19;20(12):1881-90. Epub 2012 Nov 19.

Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine , Baltimore, Maryland.

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http://dx.doi.org/10.1089/ars.2012.4963DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3967382PMC
April 2014

Early frameshift mutation in PIGA identified in a large XLID family without neonatal lethality.

Hum Mutat 2014 Mar 13;35(3):350-5. Epub 2014 Jan 13.

Human Genome Laboratory, VIB Center for the Biology of Disease, Leuven, Belgium; Human Genome Laboratory, Department of Human Genetics, KU Leuven, Leuven, Belgium.

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http://dx.doi.org/10.1002/humu.22498DOI Listing
March 2014

Extensive ex vivo expansion of functional human erythroid precursors established from umbilical cord blood cells by defined factors.

Mol Ther 2014 Feb 3;22(2):451-463. Epub 2013 Sep 3.

Division of Hematology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA; Stem Cell Program, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA. Electronic address:

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http://dx.doi.org/10.1038/mt.2013.201DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3916033PMC
February 2014

Differential sensitivity to JAK inhibitory drugs by isogenic human erythroblasts and hematopoietic progenitors generated from patient-specific induced pluripotent stem cells.

Stem Cells 2014 Jan;32(1):269-78

Division of Hematology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA; Stem Cell Program, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.

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http://dx.doi.org/10.1002/stem.1545DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4096297PMC
January 2014

Generation of glycosylphosphatidylinositol anchor protein-deficient blood cells from human induced pluripotent stem cells.

Stem Cells Transl Med 2013 Nov 10;2(11):819-29. Epub 2013 Oct 10.

Division of Hematology, Department of Medicine, School of Medicine, and.

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http://dx.doi.org/10.5966/sctm.2013-0069DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3808197PMC
November 2013

Generation and homing of iPSC-derived hematopoietic cells in vivo.

Mol Ther 2013 Jul;21(7):1292-3

Division of Hematology, Department of Medicine and Stem Cell Program, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.

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http://dx.doi.org/10.1038/mt.2013.129DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3702102PMC
July 2013

Efficient drug screening and gene correction for treating liver disease using patient-specific stem cells.

Hepatology 2013 Jun;57(6):2458-68

Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

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http://doi.wiley.com/10.1002/hep.26237
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http://dx.doi.org/10.1002/hep.26237DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3633649PMC
June 2013

Generation of integration-free human induced pluripotent stem cells from postnatal blood mononuclear cells by plasmid vector expression.

Nat Protoc 2012 Nov 18;7(11):2013-21. Epub 2012 Oct 18.

Stem Cell Program, Institute for Cell Engineering, Division of Hematology, Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.

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http://dx.doi.org/10.1038/nprot.2012.121DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3756815PMC
November 2012

Promise and challenges of human iPSC-based hematologic disease modeling and treatment.

Int J Hematol 2012 Jun 23;95(6):601-9. Epub 2012 May 23.

Division of Hematology in Department of Medicine, Johns Hopkins University School of Medicine, 720 Rutland Ave., Ross Research Building, Room 1032, Baltimore, MD 21205, USA.

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http://dx.doi.org/10.1007/s12185-012-1095-9DOI Listing
June 2012

The phenotype of a germline mutation in PIGA: the gene somatically mutated in paroxysmal nocturnal hemoglobinuria.

Am J Hum Genet 2012 Feb 2;90(2):295-300. Epub 2012 Feb 2.

Genetic Disease Research Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, USA.

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http://dx.doi.org/10.1016/j.ajhg.2011.11.031DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3276655PMC
February 2012

Hematopoietic cells as sources for patient-specific iPSCs and disease modeling.

Cell Cycle 2011 Sep 1;10(17):2840-4. Epub 2011 Sep 1.

Division of Hematology, Department of Medicine, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3218597PMC
http://dx.doi.org/10.4161/cc.10.17.17180DOI Listing
September 2011

Reprogramming of EBV-immortalized B-lymphocyte cell lines into induced pluripotent stem cells.

Blood 2011 Aug 31;118(7):1801-5. Epub 2011 May 31.

Stem Cell Biology Laboratory, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA.

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http://dx.doi.org/10.1182/blood-2011-03-340620DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3158714PMC
August 2011

Liver engraftment potential of hepatic cells derived from patient-specific induced pluripotent stem cells.

Cell Cycle 2011 Aug 1;10(15):2423-7. Epub 2011 Aug 1.

Stem Cell Biology Laboratory, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3180184PMC
http://dx.doi.org/10.4161/cc.10.15.16869DOI Listing
August 2011

Molecular imaging and stem cell research.

Mol Imaging 2011 Apr;10(2):111-22

Sidney Kimmel Comprehensive Cancer Center, JohnsHopkins University School of Medicine, Baltimore, MD 21231, USA.

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April 2011

Efficient human iPS cell derivation by a non-integrating plasmid from blood cells with unique epigenetic and gene expression signatures.

Cell Res 2011 Mar 18;21(3):518-29. Epub 2011 Jan 18.

Stem Cell Program, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.

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http://dx.doi.org/10.1038/cr.2011.12DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3193421PMC
March 2011

Potential of human induced pluripotent stem cells derived from blood and other postnatal cell types.

Regen Med 2010 Jul;5(4):521-30

Division of Hematology & Stem Cell Program, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Broadway Research Building, Room 747, 733 N. Broadway, Baltimore, MD 21205, USA.

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http://dx.doi.org/10.2217/rme.10.38DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4028827PMC
July 2010

An improved method for generating and identifying human induced pluripotent stem cells.

Methods Mol Biol 2010 ;636:191-205

Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

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http://dx.doi.org/10.1007/978-1-60761-691-7_12DOI Listing
June 2010

Human-induced pluripotent stem cells from blood cells of healthy donors and patients with acquired blood disorders.

Blood 2009 Dec 1;114(27):5473-80. Epub 2009 Oct 1.

Stem Cell Program, Institute for Cell Engineering, and Department of Gynecology & Obstetrics, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

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http://www.bloodjournal.org/cgi/doi/10.1182/blood-2009-04-21
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http://dx.doi.org/10.1182/blood-2009-04-217406DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2798863PMC
December 2009

Serial imaging of human embryonic stem-cell engraftment and teratoma formation in live mouse models.

Cell Res 2009 Mar;19(3):370-9

Department of Radiology and Radiological Sciences, Johns Hopkins School of Medicine, Baltimore, MD 21231, USA.

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http://dx.doi.org/10.1038/cr.2008.329DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6109972PMC
March 2009

In vivo functional efficacy of tumor-specific T cells expanded using HLA-Ig based artificial antigen presenting cells (aAPC).

Cancer Immunol Immunother 2009 Feb 18;58(2):209-20. Epub 2008 Jun 18.

Department of Pathology, Johns Hopkins School of Medicine, Ross 664G, 720 Rutland Avenue, Baltimore, MD 21205, USA.

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http://dx.doi.org/10.1007/s00262-008-0542-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2680120PMC
February 2009

In vivo commitment and functional tissue regeneration using human embryonic stem cell-derived mesenchymal cells.

Proc Natl Acad Sci U S A 2008 Dec 18;105(52):20641-6. Epub 2008 Dec 18.

Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.

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http://dx.doi.org/10.1073/pnas.0809680106DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2634917PMC
December 2008

Improved efficiency and pace of generating induced pluripotent stem cells from human adult and fetal fibroblasts.

Stem Cells 2008 Aug 29;26(8):1998-2005. Epub 2008 May 29.

Stem Cell Program, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Broadway Research Building, Room 747, 733 North Broadway, Baltimore, Maryland 21205, USA.

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http://dx.doi.org/10.1634/stemcells.2008-0346DOI Listing
August 2008

Lentiviral gene transduction of mouse and human stem cells.

Methods Mol Biol 2008 ;430:243-53

Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD.

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http://dx.doi.org/10.1007/978-1-59745-182-6_17DOI Listing
April 2008

How reproducible is bioluminescent imaging of tumor cell growth? Single time point versus the dynamic measurement approach.

Mol Imaging 2007 Sep-Oct;6(5):315-22

Division of Nuclear Medicine, The Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins Medical Institutions, Baltimore, MD 21287, USA.

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February 2008

FLT3/ITD expression increases expansion, survival and entry into cell cycle of human haematopoietic stem/progenitor cells.

Br J Haematol 2007 Apr;137(1):64-75

Department of Oncology, Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD, USA.

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http://dx.doi.org/10.1111/j.1365-2141.2007.06525.xDOI Listing
April 2007

Inducible and reversible transgene expression in human stem cells after efficient and stable gene transfer.

Stem Cells 2007 Mar 7;25(3):779-89. Epub 2006 Dec 7.

Stem Cell Program, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Broadway Research Building, Room 747, 733 North Broadway, Baltimore, MD 21205, USA.

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http://doi.wiley.com/10.1634/stemcells.2006-0128
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http://dx.doi.org/10.1634/stemcells.2006-0128DOI Listing
March 2007

Promoting human embryonic stem cell renewal or differentiation by modulating Wnt signal and culture conditions.

Cell Res 2007 Jan 9;17(1):62-72. Epub 2007 Jan 9.

Institute for Cell Engineering, Department of Gynecology & Obstetrics, the Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.

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http://dx.doi.org/10.1038/sj.cr.7310138DOI Listing
January 2007

Defining the role of Wnt/beta-catenin signaling in the survival, proliferation, and self-renewal of human embryonic stem cells.

Stem Cells 2005 Nov-Dec;23(10):1489-501. Epub 2005 Jul 7.

The Institute for Cell Engineering, Department of Gynecology & Obstetrics, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.

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http://doi.wiley.com/10.1634/stemcells.2005-0034
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http://dx.doi.org/10.1634/stemcells.2005-0034DOI Listing
March 2006

Electrophysiological properties of pluripotent human and mouse embryonic stem cells.

Stem Cells 2005 Nov-Dec;23(10):1526-34. Epub 2005 Aug 9.

Department of Medicine, University of Hong Kong, Hong Kong, China.

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http://dx.doi.org/10.1634/stemcells.2004-0299DOI Listing
March 2006

[Content determination of loganic acid and gentiopicroside in Tebitan herbal medicines Gentiana macrophylla and G. straminea by HPLC].

Zhong Yao Cai 2004 Nov;27(11):819-21

Qinghai Nationalities Institutes, Xining.

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November 2004

Functional antigen-presenting leucocytes derived from human embryonic stem cells in vitro.

Lancet 2004 Jul 10-16;364(9429):163-71

Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.

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http://dx.doi.org/10.1016/S0140-6736(04)16629-4DOI Listing
July 2004

Myocyte enhancer factor 2 mediates calcium-dependent transcription of the interleukin-2 gene in T lymphocytes: a calcium signaling module that is distinct from but collaborates with the nuclear factor of activated T cells (NFAT).

J Biol Chem 2004 Apr 13;279(15):14477-80. Epub 2004 Jan 13.

Department of Pharmacology and Molecular Science and Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.

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http://www.jbc.org/lookup/doi/10.1074/jbc.C300487200
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http://dx.doi.org/10.1074/jbc.C300487200DOI Listing
April 2004

Making lentiviral vectors more powerful and universal.

Discov Med 2003 Oct;3(18):48-9

Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University School of Medicine, 1650 Orleans Street, Baltimore, MD 21231, USA.

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October 2003

Human adult marrow cells support prolonged expansion of human embryonic stem cells in culture.

Stem Cells 2003 ;21(2):131-42

The Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA.

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http://dx.doi.org/10.1634/stemcells.21-2-131DOI Listing
September 2003

Lentivirus-mediated gene transfer and expression in established human tumor antigen-specific cytotoxic T cells and primary unstimulated T cells.

Hum Gene Ther 2003 Jul;14(11):1089-105

Division of Immunology and Hematppoiesis, The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA.

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http://dx.doi.org/10.1089/104303403322124800DOI Listing
July 2003

Targeting transgene expression to antigen-presenting cells derived from lentivirus-transduced engrafting human hematopoietic stem/progenitor cells.

Blood 2002 Jan;99(2):399-408

Division of Immunology and Hematopoiesis, Johns Hopkins Oncology Center, Johns Hopkins University, Baltimore, MD 21231, USA.

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http://dx.doi.org/10.1182/blood.v99.2.399DOI Listing
January 2002