Publications by authors named "Zhao-Hui Wang"

132 Publications

Treatment of primary cardiac angiosarcoma in a 35 weeks pregnant woman.

J Card Surg 2021 Jul 3. Epub 2021 Jul 3.

Department of Cardiac Surgery, People's Hospital of Dongyang, Dongyang, Zhejiang, China.

Background: Cardiac angiosarcoma is a rare but highly malignant cardiac tumor. It is characterized by poor prognosis, and current treatment approaches are not effective.

Case Presentation: A 37-year-old female with 35 weeks pregnancy experienced chest tightness and shortness of breath for 1 month. She was diagnosed with primary cardiac angiosarcoma. Delivery of fetus was performed early to treat the mother. The patient underwent resection of the tumor then she was treated with chemotherapy. However, the tumor recurred 11 months after surgery.

Conclusion: Angiosarcoma is a highly malignant tumor explaining recurrence of the tumor recurred after surgery. Cardiac angiosarcoma should be treated through a comprehensive treatment plan, comprising surgery, radiotherapy, and chemotherapy approaches.
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http://dx.doi.org/10.1111/jocs.15751DOI Listing
July 2021

Preparing Adipogenic Hydrogel with Neo-Mechanical Isolated Adipose-Derived Extracellular Vesicles for Adipose Tissue Engineering.

Plast Reconstr Surg 2021 Jun 21. Epub 2021 Jun 21.

From the Department of Plastic Surgery, The Second Affiliated Hospital of Nanchang University.

Background: Adipose tissue engineering greatly reduce the burden of reconstruction surgery. Subcutaneous transplantation of decellularized adipose tissue was capable of recellularization. However, further improvements are required to promote angiogenesis and adipogenesis. Thus, the authors proposed a neo-mechanical protocol to isolate adipose tissue-derived extracellular vesicles (ATEVs) from liposuction as a factor for both angiogenesis and adipogenesis, and prepared ATEV-rich decellularized adipose tissue hydrogel for adipose tissue engineering.

Methods: Adipose liquid extract and lipid-devoid adipose tissue were extracted by means of homogenization and repeated freezing and thawing. ATEVs were isolated from adipose liquid extract by ultracentrifugation. Decellularized adipose tissue hydrogel was prepared by optimized decellular technique. The optimum dose of ATEVs for promoting angiogenesis and adipogenesis was screened out by co-culture with vascular endothelial cells and 3T3-L1 cells, then mixed with the hydrogel at the optimal dose. ATEV-enriched hydrogel was injected subcutaneously into the right back of severe combined immunodeficiency mice, and hydrogel was injected as a control on the contralateral side. ATEVs were supplementarily injected into the newborn tissue on the right back at postoperative day 14, and the same amount of phosphate-buffered saline was injected into the left as a control. The samples of both sides were harvested at postoperative weeks 2, 4, and 8 and subjected to volume measure, hematoxylin and eosin staining, and immunofluorescence (CD31 and perilipin) staining.

Results: The optimum dose of ATEVs for promoting angiogenesis and adipogenesis was 50 μg/ml. In vivo, supplementing ATEVs increased the volume of decellularized adipose tissue hydrogel specimens compared with control specimens at postoperative week 4 (99.4 ± 22.5 μl versus 126.1 ± 16.7 μl; n = 8; p < 0.05) and week 8 (61.2 ± 25.5 μl versus 80.9 ± 23.1 μl; n = 8; p < 0.05); promoted angiogenesis at postoperative week 2 (1243.8 ± 418.7 μm versus 702.2 ± 283.3 μm; n = 8; p < 0.05), week 4 (1975.2 ± 476.3 μm versus 1459.2 ± 398.6 μm; n = 8; p < 0.05), and week 8 (2068.9 ± 407.1 μm versus 1593.9 ± 320.3 μm; n = 8; p < 0.05); and improved the adipogenesis at postoperative week 4 (15.1 ± 7.4 percent versus 2.9 ± 1.9 percent; n = 8; p < 0.05) and week 8 (45.5 ± 13.1 percent versus 20.5 ± 6.5 percent; n = 8; p < 0.05).

Conclusion: ATEV-enriched adipogenic hydrogel encourages enhanced angiogenesis and adipogenesis and could serve as a promising biomaterial for adipose tissue engineering.
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http://dx.doi.org/10.1097/PRS.0000000000008186DOI Listing
June 2021

[Acupuncture preconditioning at "Zusanli"(ST36) and "Zhongwan"(CV12) prevents stress gastric ulcer by regulating the TLR4/MyD88/IκB signaling pathway].

Zhen Ci Yan Jiu 2021 Mar;46(3):173-9

School of Acupuncture-moxibustion and Tuina, Changchun University of Chinese Medicine, Changchun 130117, China.

Objective: To observe the effect of electroacupuncture preconditioning at "Zusanli"(ST36,Lower Confluent point) and "Zhongwan"(CV12,Front-Mu point) combination on oxidative stress and inflammation-related indicators, Toll-like receptor 4 (TLR4), myeloid differentiation factor 88 (MyD88) and inhibitor-α of nuclear transcription factor κB (IκB-α) in serum and gastric tissue of rats with stress gastric ulcer(SGU),so as to explore its mechanisms underlying prevention of SGU.

Methods: A total of 36 Wistar rats were randomly divided into blank control, model, positive drug and He-Sea-Front-Mu point combination groups (=9 in each group). A rat model of SGU was established by restraint water-immersion stress method. Ten days before mode-ling, rats in the He-Sea-Front-Mu point combination group received electroacupuncture (2 Hz, 0.6 mA)at ST36 and CV12 for 10 min once every other day for 10 days, and those in the positive drug group was treated by gavage of omeprazole (20 mg/kg) once every other day for 10 days. The morphology of the gastric mucosa was observed by naked eyes and hematoxylin-eosin staining, and the ulcer index (UI) and lesion score were calculated. TBA and colorimetric methods, ELISA and Western blot were used to detect malondialdehyde (MDA), myeloperoxidase (MPO), glutathione peroxidase (GSH-Px), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) levels and the relative expressions of TLR4, MyD88, and IκB-α protein, separately.

Results: The gastric mucosa of rats in the blank control group was smooth and intact, the cells were arranged neatly, and there was no telangiec-tasia, hyperemia and inflammatory cell infiltration. The gastric mucosal epithelial structure of rats in the model group was destroyed, and a large number of mucosal epithelial cell death and inflammatory cell infiltration were seen. The degree of gastric mucosal injury and inflammatory cell infiltration in the positive drug group and the combined point group was less than that in the model group. Compared with the blank control group, the UI and lesion score of rats in the model group were significantly increased (<0.05), the levels of MDA and MPO in the serum and gastric tissues were significantly increased (<0.05), GSH-Px was significantly reduced (<0.05), the contents of TNF-α and IL-6 in serum were markedly increased (<0.05), the expression levels of TLR4 and MyD88 proteins in gastric tissue were significantly increased (<0.05), IκB-α was significantly reduced (<0.05). After intervention and in comparison with the model group showed that, the UI and lesion score, the levels of MDA and MPO, contents of serum TNF-α and IL-6, expression levels of TLR4 and MyD88 proteins in positive drug and He-Sea-Front-Mu point combination groups were significantly decreased (<0.05), while GSH-Px and IκB-α were significantly increased (<0.05); There were no significant differences in the above indicators between the positive drug and the He-Sea-Front -Mu point combination groups (except TNF-α).

Conclusion: Electroacupuncture preconditioning at ST36 and CV12 can prevent SGU, which may be related to its effects in anti-oxidant, anti-inflammatory and regulating TLR4/MyD88/IκB signaling pathway.
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http://dx.doi.org/10.13702/j.1000-0607.200963DOI Listing
March 2021

Astilbin Protects Against Carbon Tetrachloride-Induced Liver Fibrosis in Rats.

Pharmacology 2021 29;106(5-6):323-331. Epub 2021 Mar 29.

Department of liver disease, Qingdao No.6 People's Hospital, Shandong Province, China.

Background: Hepatic fibrosis is an inflammatory liver disease, and there is no effective therapy at present. Astilbin is a bioactive ingredient found in many medicinal and food plants, with antioxidative, anti-inflammatory, and antitumor properties.

Objectives: This study aimed to investigate the protective effect and related molecular mechanism of astilbin against carbon tetrachloride (CCl4)-induced liver fibrosis in rats.

Methods: Liver fibrosis was induced by injection of CCl4 in male Sprague-Dawley rats, and those rats were then treated with astilbin at different concentrations. Pathological changes, collagen production, inflammatory cytokine, and oxidative stress were evaluated to evaluate the effects of astilbin on CCl4-induced hepatic fibrosis. Real-time PCR and western blot were performed to detect the mRNA and protein expression of indicated genes.

Results: We discovered that CCl4 caused significant fibrosis damage in rat liver, and astilbin dose-dependently improved the liver functions and fibrosis degree. Astilbin treatment significantly decreased collagen production, inflammatory response, and oxidative stress in vivo. Mechanically, administration of astilbin obviously elevated the hepatic levels of Nrf2 and its downstream components, including NAD(P)H:quinone oxidoreductase 1 (Nqo1), heme oxygenase (HO-1), glutamate-cysteine ligase catalytic subunit, and glutamate cysteine ligase modifier.

Conclusions: Taken together, these findings demonstrate that astilbin could protect against CCL4 induced-liver fibrosis in rats.
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http://dx.doi.org/10.1159/000514594DOI Listing
July 2021

Ruthenium(II)-Catalyzed C-C/C-N Coupling of 2-Arylquinazolinones with Vinylene Carbonate: Access to Fused Quinazolinones.

Org Lett 2021 Feb 27;23(3):995-999. Epub 2021 Jan 27.

School of Chemistry and Materials Science, Liaoning Shihua University, Fushun 113001, P. R. China.

In this work, ruthenium(II)-catalyzed C-C/C-N annulation of 2-arylquinazolinones with vinylene carbonate is reported to synthesize fused quinazolinones. This catalytic system tolerates a wide range of substrates with excellent functional-group compatibility. In this transformation, the vinylene carbonate acts as an ethynol surrogate without any external oxidant involved. Furthermore, preliminary mechanistic studies were conducted, and a plausible catalytic cycle was also proposed.
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http://dx.doi.org/10.1021/acs.orglett.0c04200DOI Listing
February 2021

[Medication rules of traditional Chinese medicine for the treatment of premature ejaculation: An analysis based on data mining].

Zhonghua Nan Ke Xue 2020 Jul;26(7):650-655

The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510405, China.

Objective: To study the medication rules of traditional Chinese medicine (TCM) in the treatment of premature ejaculation.

Methods: We searched the databases of CNKI, Wan Fang, VIP, SinoMed (CBM) and PubMed for studies on the treatment of PE with TCM prescriptions and performed statistical analyses on the data obtained using the TCM inheritance auxiliary platform software.

Results: Totally 180 prescriptions were identified, involving 209 TCM drugs. The results of statistical analysis showed that the TCM drugs for the treatment of premature ejaculation were mostly warm, flat or cold in nature, sweet or spicy in taste, and with the kidney, liver and spleen meridian tropisms. The single TCM drugs most commonly used included Lycium barbarum L (Gouqizi), followed by Epimedium brevicornum Maxim (Yinyanghuo), Os draconis (Longgu), Fructus rosae laevigatae (Jinyingzi), and the drugs most frequently used in combination with others in a prescription were Os draconis (Longgu) and Concha ostreae (Muli). Seven newly derived prescriptions were identified in addition.

Conclusions: The compatibility of TCM in the treatment of premature ejaculation is characterized by the combination of the drugs for tonifying the kidney and arresting seminal emission as the main medication strategy, with those for soothing the liver and invigorating the spleen as the adjuvant agents, which has a certain clinical application value.
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July 2020

Analysis of Risk Factors for Lymph Node Metastases in Elderly Patients with Papillary Thyroid Micro-Carcinoma.

Cancer Manag Res 2020 11;12:7143-7149. Epub 2020 Aug 11.

Head and Neck Surgery Center, Sichuan Cancer Hospital and Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu 610041, People's Republic of China.

Background: With guidance from the American Joint Committee on Cancer (AJCC) Cancer Staging Manual, 8th edition, we explored the characteristics of central lymph node metastasis (CLNM) of papillary thyroid micro-carcinoma (PTMC) in elderly patients ≥55 years of age. Our goal was to provide references for establishing a lymph node dissection scheme in such patients.

Methods: We retrospectively analyzed the clinical data of thyroid cancer patients admitted to the Head and Neck Surgery Center of Sichuan Cancer Hospital, Chengdu, China, from January 2015 to September 2018. Then, we screened and analyzed eligible PTMC cases in strict accordance with our inclusion and exclusion criteria.

Results: The study included 107 patients, including 24 men and 83 women. Median age was 59.99 ± 4.58 years. The maximum diameter range of the cancer foci was 4-10 mm, and the median was 7.59 ± 1.78 mm. Unilateral lobectomy had been performed in 32 cases, total thyroidectomy in 75 cases and lateral cervical lymph node dissection in 21 cases. There were 60 cases of CLNM (56.07%) and 13 cases of lateral cervical lymph node metastasis (12.10%). The sensitivity of preoperative ultrasound in predicting CLNM was 100%, but its accuracy was only 50.47%. Multivariate logistic regression analysis showed that multiple cancer foci (area under the curve [AUC] = 0.632), extra-thyroidal expansion of cancer focus (AUC = 0.721), and irregular nodules (AUC = 0.603) were independent risk factors for CLNM of PTMC in elderly patients (P < 0.05). Overall predictability for PTMC-CLNM was 80.30%.

Conclusion: 1) Preoperative color Doppler ultrasound is not recommended as the basis for cervical lymph node dissection in PTMC patients. 2) For multiple cancer foci, irregular nodules, and elderly patients with PTMC extra-thyroidal expansion, we recommend a prophylactic central lymph node dissecting. 3) Nonsurgical observation of PTMC in elderly patients with low risk should be carefully selected.
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http://dx.doi.org/10.2147/CMAR.S248374DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7429112PMC
August 2020

Application of Computer-Aided Design (CAD) and Three-Dimensional (3D) Visualization Technologies in the Diagnosis and Treatment of Refractory Thyroid Tumors.

Cancer Manag Res 2020 5;12:6887-6894. Epub 2020 Aug 5.

Department of Head and Neck Oncology, Sichuan Cancer Hospital, Chengdu 610041, People's Republic of China.

Background: To evaluate the application of computer-aided design (CAD) and three-dimensional (3D) visualization techniques in the diagnosis and treatment of refractory thyroid tumors.

Materials And Methods: The clinical data from 12 cases of refractory thyroid tumors treated with CAD and 3D visualization techniques from September 2016 to January 2018 were analyzed retrospectively. Ten cases were malignant, while two cases were benign. All tracheas in the 12 cases were invaded or oppressed by the thyroid tumors. Six of the cases experienced type III dyspnea, while the other six cases had type II dyspnea. All patients underwent contrast-enhanced computed tomography (CT) imaging.

Results: CAD and 3D visualization technologies were used to guide the surgeries. Ten cases of malignant thyroid tumors underwent total thyroidectomy, cervical lymph node dissection, and invaded organ resection. Among the ten cases, five underwent sleeve resections and anastomosis reconstructions, one underwent a tracheal sleeve resection and total laryngectomy, two underwent tracheal window resections, one case underwent a sternotomy, and one case underwent a laryngopharyngectomy, invaded skin resection, and pectoralis major muscle flap reconstruction. The two cases of benign thyroid tumors underwent subtotal thyroidectomies. Using CAD and 3D visualization techniques, surgeons can visually observe the relationship of the tumor with the respiratory tract and essential blood vessels, which can lead to optimized surgical plans and improved surgical outcomes.

Conclusion: CAD and 3D visualization technologies have an important role in the personalized surgical intervention of thyroid cancer, which will likely have important clinical implications.
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http://dx.doi.org/10.2147/CMAR.S246576DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7415464PMC
August 2020

A Cross-Reference Line Method Based Multiobjective Evolutionary Algorithm to Enhance Population Diversity.

Comput Intell Neurosci 2020 18;2020:7179647. Epub 2020 Jul 18.

Department of Mechanical Engineering, Institute of Manufacturing Engineering, Tsinghua University, Beijing 100084, China.

Multiobjective evolutionary algorithms (MOEAs) with higher population diversity have been extensively presented in literature studies and shown great potential in the approximate Pareto front (PF). Especially, in the recent development of MOEAs, the reference line method is increasingly favored due to its diversity enhancement nature and auxiliary selection mechanism based on the uniformly distributed reference line. However, the existing reference line method ignores the nadir point and consequently causes the Pareto incompatibility problem, which makes the algorithm convergence worse. To address this issue, a multiobjective evolutionary algorithm based on the adaptive cross-reference line method, called MOEA-CRL, is proposed under the framework of the indicator-based MOEAs. Based on the dominant penalty distance (DPD) indicator, the cross-reference line method can not only solve the Pareto incompatibility problem but also enhance the population diversity on the convex PF and improve the performances of MOEA-CRL for irregular PF. In addition, the MOEA-CRL adjusts the distribution of the cross-reference lines directly defined by the DPD indicator according to the contributing solutions. Therefore, the adaptation of cross-reference lines will not be affected by the population size and the uniform distribution of cross-reference lines can be maintained. The MOEA-CRL is examined and compared with other MOEAs on several benchmark problems. The experimental results show that the MOEA-CRL is superior to several advanced MOEAs, especially on the convex PF. The MOEA-CRL exhibits the flexibility in population size setting and the great versatility in various multiobjective optimization problems (MOPs) and many-objective optimization problems (MaOPs).
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http://dx.doi.org/10.1155/2020/7179647DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7388677PMC
July 2021

Surface configuration of CO adsorbed on nanostructured Pt electrodes probed using broadband sum frequency generation spectroscopy.

Chem Commun (Camb) 2020 Aug;56(67):9723-9726

Collaborative Innovation Center of Chemistry for Energy Materials, State Key Laboratory for Physical Chemistry of Solid Surface, Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.

Using broadband sum frequency generation (BB-SFG) spectroscopy, the effect of surface structure on the adsorption states of linearly bonded CO (COL) on a Pt electrode was thoroughly analyzed. Two overlapped SFG peaks with different linewidths and electrochemical Stark slopes were identified, which correspond to COL in different surface configurations.
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http://dx.doi.org/10.1039/d0cc02469dDOI Listing
August 2020

Familial Atrial Enlargement, Conduction Disorder and Symmetric Cardiac Hypertrophy Are Early Signs of PRKAG2 R302Q.

Curr Med Sci 2020 Jun 17;40(3):486-492. Epub 2020 Jul 17.

Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.

PRKAG2 cardiac syndrome (PS) is a rare inherited disease due to PRKAG2 gene mutation and characterized by Wolff-Parkinson-White syndrome (WPWs), conduction system lesions and myocardial hypertrophy. It can also lead to serious consequences, such as sudden death. But the genetic and clinical heterogeneity makes the early diagnosis of PS difficult. Here we studied a family with familial hypertrophic cardiomyopathy and other diverse manifestations. Gene analysis identified a missense mutation (Arg302Gln) in the five affected subjects of the family. The electrocardiograph performance of the five was composed of sinus bradycardia (SB), WPWs, right bundle branch block (RBBB), atrioventricular block (AVB), left bundle branch block (LBBB), supraventricular tachycardia (SVT) and atrial premature beat (APB). Among them, the youngest one began to show paroxysmal palpitation at the age of nine and was confirmed to have WPWs at 17 years old; two members progressed over time to serious conduction damage, and the proband received a pacemaker at the age of 27 due to AVB. Besides, according to cardiac magnetic resonance and echocardiography, the youngest one showed symmetric hypertrophy; three older members showed asymmetric myocardial hypertrophy characterized with a diffuse pattern of middle-anterior-lateral-inferior wall hypertrophy and especially interventricular septal hypertrophy; all five affected patients showed atrial enlargement regardless of myocardial hypertrophy at an earlier stage. In conclusion, the conduction system disorder, familial atrial enlargement and symmetric cardiac hypertrophy may occur in the early stage of PRKAG2 R302Q mutation.
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http://dx.doi.org/10.1007/s11596-020-2207-zDOI Listing
June 2020

Cardiac Troponin I Is an Independent Predictor for Mortality in Hospitalized Patients With COVID-19.

Circulation 2020 08 15;142(6):608-610. Epub 2020 Jun 15.

Departments of Cardiology (S.-F.N., M.Y., T.X., F.Y., Z.-H.W., M.L., X.-L.G., B.-J.L., S.-J.W., X.-B.Z., S.-O.H., Y.-H.L., Z.-H.Z., X.C.), Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

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http://dx.doi.org/10.1161/CIRCULATIONAHA.120.048789DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7418761PMC
August 2020

Supplementation with Extracellular Vesicles Derived from Adipose-Derived Stem Cells Increases Fat Graft Survival and Browning in Mice: A Cell-Free Approach to Construct Beige Fat from White Fat Grafting.

Plast Reconstr Surg 2020 05;145(5):1183-1195

From the Department of Plastic Surgery, The Second Affiliated Hospital of Nanchang University.

Background: Growing evidence has demonstrated that adipose-derived stem cell-derived extracellular vesicles enhance the survival of fat grafts and the browning of white adipose tissue. We evaluated whether supplementation with adipose-derived stem cell-derived extracellular vesicles promotes the survival and browning of fat grafts.

Methods: Extracellular vesicles derived from adipose-derived stem cells were injected into fat grafts of C57BL/6 mice once per week until postgraft week 12. The grafts were collected and weighed after postgraft weeks 2, 4, and 12. The histological morphology, neovascularization, and the proportion of M2 macrophages of grafts were evaluated. The ability of extracellular vesicles to promote macrophage polarization and catecholamine secretion was detected. Whether the inducement of browning adipose differentiation is extracellular vesicles or the paracrine effect of M2 macrophages polarized by extracellular vesicles was also verified.

Results: Grafts treated by extracellular vesicles derived from adipose-derived stem cells showed enhanced beige adipose regeneration with increased neovascularization, M2 macrophage proportion, and norepinephrine secretion at postgraft week 4. Increased retention and decreased fibrosis and necrosis were noted at postgraft week 12. The extracellular vesicles uptake by macrophages promoted M2 type polarization and catecholamine secretion while suppressing M1 type polarization. Of note, browning adipose differentiation with enhanced energy expenditure could be promoted only by the conditioned medium from extracellular vesicle-polarized M2 macrophages but not by extracellular vesicles themselves.

Conclusions: Supplementation with extracellular vesicles derived from adipose-derived stem cells increases fat graft survival and browning by which extracellular vesicles-polarized M2 macrophages secrete catecholamines to promote beige adipose regeneration.
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http://dx.doi.org/10.1097/PRS.0000000000006740DOI Listing
May 2020

Extracellular Vesicles Derived From Human Adipose-Derived Stem Cell Prevent the Formation of Hypertrophic Scar in a Rabbit Model.

Ann Plast Surg 2020 05;84(5):602-607

From the Department of Plastic Surgery, The Second Affiliated Hospital of Nanchang University, Jiangxi, P.R. China.

Background: Preventing scar formation during wound healing has important clinical implications. Numerous studies have indicated that adipose-derived stem cell culture mediums, which are rich in cytokines and extracellular vesicles (EVs), regulate matrix remodeling and prevent scar formation after wound healing. Therefore, using a rabbit scar model, we tried to demonstrate which factor in adipose-derived stem cell culture mediums plays a major role in preventing scar formation (EVs or cytokines), as well as revealing the underlying mechanism.

Methods: Human adipose-derived stem cells (hASCs) were isolated from the subcutaneous adipose tissue of a healthy female donor. The surface CD markers of third-passage hASCs were analyzed by flow cytometry. The adipogenic differentiation capacity of the hASCs was detected using Oil O staining. A cultured medium of third- to five-passage hASCs was collected for EV and EV-free medium isolations. Extracellular vesicles were characterized using transmission electron microscopy, NanoSight, and the Western blotting for surface markers CD63, TSG101, and Alix. The EV-free medium was characterized by Western blotting for vascular endothelial growth factor A (VEGFA), platelet derived growth factor B (PDGFB), and transforming growth factor β 1 (TGFβ1). Eight-millimeter-diameter wounds were created on the ventral side of both ears of 16 New Zealand rabbits. A total of 0.1 mL of the human adipose-derived stem cell-extracellular vesicle (hASC-EV) or EV-free medium was locally injected into wounds made on the right ears during wound healing. Meanwhile, equal amounts of phosphate buffer saline were injected into the left ears as a control. Biopsies of the wounded skin and surrounding tissue were excised on postoperative day 28 and subjected to hematoxylin and eosin (H&E), Masson, and α-SMA immunofluorescence staining. The protein expression of α-SMA and collagen I in both scar tissues and the normal skin were evaluated via Western blotting.

Results: The hASCs expressed high levels of 49d, CD90, CD105, and CD73 but did not express CD34 or CD45. The hASCs differentiated into adipocytes under an adipogenic induction medium. Under transmission electron microscopy, the hASC-EVs were circular, bilayer membrane vesicles and approximately 95% of the particles were between 50 and 200 nm in size. The hASC-EVs expressed the same surface markers as EVs, including CD63, TSG101, and Alix and displayed little expression of VEGFA, PDGFB, and TGFβ1. The EV-free medium had a high expression of VEGFA, PDGFB, and TGFβ1 but displayed no expression of CD63, TSG101, and Alix. In vivo, the hASC-EV treatment prevented the formation of hypertrophic scars on postoperative day 28 and suppressed collagen deposition and myofibroblast aggregation. However, the EV-free medium did not prevent the formation of hypertrophic scars on the same time point and had little effect on collagen deposition and myofibroblast aggregation when compared with the control group.

Conclusions: Our study suggests that hASCs are associated with preventive scar formation therapy because of paracrine EVs rather than cytokines. A local injection of hASC-EVs during wound healing efficiently prevented hypertrophic scar formation, which may have a clinically beneficial antiscarring effect.
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http://dx.doi.org/10.1097/SAP.0000000000002357DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7357540PMC
May 2020

Factors influencing left ventricular ejection fraction in patients with coronary microvascular disease and obstructive coronary artery disease.

BMC Res Notes 2020 Mar 16;13(1):157. Epub 2020 Mar 16.

Tongji Medical College, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan, 43000, Hubei, China.

Objective: The aim of our research was to evaluate the relationship involving left ventricular ejection fraction, low density lipoprotein, B-type natriuretic peptide, Troponin I and coronary flow reserve, and to determine the predictors of left ventricular ejection fraction in patients with coronary microvascular disease and obstructive coronary artery disease, and in patients with coronary microvascular disease.

Results: The mean age was 58.5 ± 12.5 years. In patients with obstructive coronary disease and coronary microvascular disease we found low density lipoprotein-c had significant inverse relationship with left ventricular ejection fraction, left ventricular ejection fraction also had significant negative relationship with B-type natriuretic peptide, and Troponin-I. While a significant direct relationship turned out to be observed linking left ventricular ejection fraction with coronary flow reserve. Left ventricular ejection fraction had significant negative relationship with low density lipoprotein, and B-type natriuretic peptide in patients with obstructive coronary artery disease only. Age, blood pressure, lipid levels, red cell distribution width, glycated hemoglobin, symptoms, New York heart association classification, alcohol drinking, hypertension, diabetes mellitus, troponin levels and B-type natriuretic peptide were the predictors for left ventricular ejection fraction in coronary microvascular disease patients.
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http://dx.doi.org/10.1186/s13104-020-05008-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7077157PMC
March 2020

Synthesis, self-assembly and nonlinear optical activity of selenium-annulated perylene diimide.

Chem Commun (Camb) 2020 Mar;56(21):3123-3126

School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, P. R. China.

A novel Se-annulated perylene diimide derivative tethered with polyhedral oligosilsesquioxane (POSS) nanoparticles (POSS-2SePDI-POSS) was designed and prepared. The introduction of selenium atoms endows POSS-2SePDI-POSS with significant fluorescence quenching but enhanced excited-state absorption. As a result, POSS-2SePDI-POSS exhibits a distinct reverse saturable absorption characteristic, suggesting its potential application in optical limiting.
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http://dx.doi.org/10.1039/c9cc10006gDOI Listing
March 2020

Bolus norepinephrine and phenylephrine for maternal hypotension during elective cesarean section with spinal anesthesia: a randomized, double-blinded study.

Chin Med J (Engl) 2020 Mar;133(5):509-516

Department of Anesthesiology, Women's Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, Jiangsu 210004, China.

Background: In recent years, norepinephrine has attracted increasing attention for the management of maternal hypotension during elective cesarean section with spinal anesthesia. Intermittent bolus is a widely used administration paradigm for vasopressors in obstetric anesthesia in China. Thus, in this randomized, double-blinded study, we compared the efficacy and safety of equivalent bolus norepinephrine and phenylephrine for rescuing maternal post-spinal hypotension.

Methods: In a tertiary women's hospital in Nanjing, China, 102 women were allocated with computer derived randomized number to receive prophylactic 8 μg norepinephrine (group N; n = 52) or 100 μg phenylephrine (group P; n = 50) immediately post-spinal anesthesia, followed by an extra bolus of the same dosage until delivery whenever maternal systolic blood pressure became lower than 80% of the baseline. Our primary outcome was standardized maternal cardiac output (CO) reading from spinal anesthesia until delivery analyzed by a two-step method. Other hemodynamic parameters related to vasopressor efficacy and safety were considered as secondary outcomes. Maternal side effects and neonatal outcomes were collected as well.

Results: Compared to group P, women in group N had a higher CO (standardized CO 5.8 ± 0.9 vs. 5.3 ± 1.0 L/min, t = 2.37, P = 0.02) and stroke volume (SV, standardized SV 73.6 ± 17.2 vs. 60.0 ± 13.3 mL, t = 4.52, P < 0.001), and a lower total peripheral resistance (875 ± 174 vs. 996 ± 182 dyne·s/cm, t = 3.44, P < 0.001). Furthermore, the incidence of bradycardia was lower in group N than in group P (2% vs. 14%, P = 0.023), along with an overall higher standardized heart rate (78.8 ± 11.6 vs. 75.0 ± 7.3 beats/min, P = 0.049). Other hemodynamics, as well as maternal side effects and neonatal outcomes, were similar in two groups (P > 0.05).

Conclusions: Compared to equivalent phenylephrine, intermittent bolus norepinephrine provides a greater CO for management of maternal hypotension during elective cesarean section with spinal anesthesia; however, no obvious maternal or neonatal clinical advantages were observed for norepinephrine.
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http://dx.doi.org/10.1097/CM9.0000000000000621DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7065858PMC
March 2020

[Effects of Warming and Straw Application on Soil Microbial Biomass Carbon and Nitrogen and Bacterial Community Structure].

Huan Jing Ke Xue 2019 Oct;40(10):4718-4724

School of Applied Meteorology, Nanjing University of Information Science and Technology, Nanjing 210044, China.

In order to investigate the effects of warming and straw application on soil microbial biomass carbon and nitrogen and bacterial community structure, a randomized block experiment was performed. Four treatments were included, namely a control (CK), warming (WA), straw application (SA), and warming and straw application (WS) treatments. The soils were sampled during the soybean (Sep. 23, 2017) and winter wheat (April 21, 2018) growing seasons. The soils were used to determine the microbial biomass C and N content using chloroform fumigation methods, and the bacterial community structure was evaluated using high-throughput sequencing (Illumina HiSeq). Results indicated that there was no significant difference in microbial biomass C between different warming and straw application treatments (>0.05). The microbial biomass N of the warming treatment was significantly higher than that of control in the soybean field (<0.01). There were significant differences in the most dominant soil bacteria between treatments in the soybean growing season (<0.05) at the class, order, family, and genus levels, while there was no difference in the winter wheat growing season. The percentages of dominant Gemmatimonadales, Gemmatimonadaceae, and in the CK and WA (or SA) treatments were significantly different (<0.05) in the soybean growing season. There was a significant (<0.05) difference in the dominant Gammaproteobacteria between the CK and WA treatments in the winter wheat growing season. The observed number of species, Shannon index, Simpson index, and Chao1 index were lowest in the warming plots in the soybean growing season and highest in the warming and straw application plots in the winter wheat growing season. The Shannon index for the WA plots was significantly higher than in the WS plots in the soybean growing season (<0.05). The observed number of species, Shannon index, Simpson index, and Chao1 index were significantly higher in the soybean plots than in the winter wheat plots (<0.05), while the abundance was significantly higher in the winter wheat plots than in the soybean plots (<0.05). The soybean growing season had significantly higher diversity than the winter wheat growing season. The indexes of diversity were highly significantly correlated with soil microbial biomass C and N in the soybean growing season (<0.001), while there was no such correlation in the winter wheat growing season. The indices of diversity were significantly correlated in both the soybean growing season and winter wheat growing season (<0.05).
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http://dx.doi.org/10.13227/j.hjkx.201902035DOI Listing
October 2019

Alkaline phosphatase activity in the phosphorus-limited southern Chinese coastal waters.

J Environ Sci (China) 2019 Dec 7;86:38-49. Epub 2019 May 7.

College of Life Science and Technology, Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, Jinan University, Guangzhou 510632, China.

Three fractions of alkaline phosphatase activity (APA), including phytoplankton APA (phyto-APA), bacterial APA (bact-APA), and free-APA, were examined in the sea surface microlayer (SML) and the subsurface water (SSW) from Daya Bay, Guishan Island, and Guanghai Bay of southern China. Relationships between APA and environmental parameters were analyzed. The growth of phytoplankton was significantly limited by dissolved inorganic phosphorus (DIP) in the three sea areas, especially in Daya Bay. Total-APA ranged between 1.41 and 35.26 nmol/L/hr, and the highest value was found in Daya Bay. The increased APA in Daya Bay was the result of the increase of phytoplankton biomass and the response of phytoplankton to P limitation. Phyto-APA was the main contributor in Daya Bay, while phyto- and free-APA co-dominated in Guishan Island and Guanghai Bay. Bact-, phyto-, and total-APA showed a significant inverse power function relationship with DIP, and 0.2 μmol/L was the threshold for DIP on particulates and total-APA. Pearson correlation analysis suggested that DIP limitation together with high N levels enhanced APA. High water temperature and freshwater input accelerated APA as well. Principal component analysis clearly separated samples from the three sea areas, as well as from the SML and the SSW, which indicated the differences in environmental parameters and APA levels. Our results highlight the influence of phosphorus limitation and environmental parameters on APA.
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http://dx.doi.org/10.1016/j.jes.2019.04.026DOI Listing
December 2019

Electrochemical CO reduction on Cu and Au electrodes studied using in situ sum frequency generation spectroscopy.

Phys Chem Chem Phys 2019 Dec 6;21(45):25047-25053. Epub 2019 Nov 6.

Collaborative Innovation Center of Chemistry for Energy Materials, State Key Laboratory for Physical Chemistry of Solid Surface, Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.

As an important pathway for energy storage and a key reaction in the carbon cycle, the CO electrochemical reduction reaction has recently gained significant interest. A variety of catalysts have been used to approach this topic experimentally and theoretically; however, the molecular level insight into the reaction mechanism is lacking due to the complexity of the surface processes and the challenges in probing the intermediate species. In this study, CO reduction reactions on polycrystalline Cu and Au electrodes were investigated in 0.1 M CO-saturated NaHCO solution. In situ sum frequency generation (SFG) spectroscopy has been adopted to access the intermediates and products on the metal electrodes. On the Au electrode, only linearly adsorbed CO could be detected, and the reduction produced no hydrocarbon species. On the Cu electrode, C-H stretching vibrations corresponding to surface-adsorbed ethoxy species were observed, but no CO vibrations can be detected with SFG. The results revealed that the CO randomly adsorbed on the Cu surface, and the multiple orientations of the adsorbed species may be the reason for the formation of C-C bonding. These results demonstrate direct molecular level evidence for different reaction pathways on the Cu and Au electrodes.
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http://dx.doi.org/10.1039/c9cp04346bDOI Listing
December 2019

Clinical characteristics and biomarkers of coronary microvascular dysfunction and obstructive coronary artery disease.

J Int Med Res 2019 Dec 9;47(12):6149-6159. Epub 2019 Aug 9.

Department of Cardiovascular Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

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http://dx.doi.org/10.1177/0300060519859134DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7045648PMC
December 2019

[Expression of MiR-155 in Tissue of Patients with Diffuse Large B-Cell Lymphoma and Its Effect on Cell Biological Characteristics].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2019 Apr;27(2):445-451

Department of Hemotology, The Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong Province ,

Objective: To investigate the expression of miR-155 in patients with diffuse large B-cell lymphoma (DLBCL), and to explore the effect of transfection of miR-155 inhibitor on the biological characteristics of DLBCL cells.

Methods: A total of 76 patients with DLBCL treated in our hospital were selected from April 2013 to December 2017. In the same time, 40 cases of lymph node reactive hyperplasia (LNRH) were selected as control group. DB cells were cultured and divided into miR-155 inhibitor, negative control and blank groups. The expressions of miR-155 in DLBCL, negative and blank control groups were detected by using real-time PCR, the cell proliferation was detected by MTT assay, the apoptosis was detected by flow cytometry, and the cell migration and invasion were detected by Transwell assay.

Results: The relative expression level of miR-155 in tissues of DLBCL patients was significantly higher than that in tissne of controls (1.93±0.16 vs 1.01±0.09) (t=33.991, P=0.000). The expression level of miR-155 increased (P<0.05) in DLBCL patients with LDH level abnormarity, BCL-2, MUM1, Ki-67≥50%, non-GC type, Ann Arbor stage III-IV, extranodal lesion number≥2 and IPI score 3-5. The relative expression level of miR-155 in the miR-155 inhibitor group was lower than that in the negative control group and the blank group (P<0.05). The absorbance (A) values at 24, 48, 72 and 96 h of culture in the miR-155 inhibitor group were lower than those in the negative control group and the blank group (P<0.05), while the apoptotic rate was higher than that in the negative control group and the blank group (P<0.05). Both the migrating cells and invading cell number in the miR-155 inhibitor group were lower than those in the negative control group and the blank group (P<0.05).

Conclusion: The miR-155 highly expresses in DLBCL tissue, which relates with tumor malignancy and invasion progression. The specific inhibition of miR-155 expression in DB cells can reduce cell proliferation, accelerate cell apoptosis, and inhibit cell migration and invasion.
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http://dx.doi.org/10.19746/j.cnki.issn.1009-2137.2019.02.022DOI Listing
April 2019

[Effects of cultivation patterns on wheat yield and soil fertility in dryland].

Ying Yong Sheng Tai Xue Bao 2019 Feb;30(2):573-582

College of Natural Resources and Environment, Northwest A&F University/Key Laboratory of Plant Nutrition and Agro-environment in Northwest China, Ministry of Agriculture, Yangling 712100, Shaanxi, China.

A field experiment was conducted to examine the effects of plastic film mulching (PM), straw retention (SR), and planting green manure (GM) on winter wheat grain yield and soil fertility. The results showed that PM did not have consistently positive effect on the grain yield, when compared to the traditional patterns (TP). No difference of average grain yield was observed between them over three years. Soil total nitrogen (N), available phosphorus (P), potassium (K), sulfur (S), zinc (Zn) and manganese (Mn) were decreased in 20-40 cm layer in PM than those in TP, while no difference was observed for soil organic matter, nitrate (NO-N), available iron (Fe) and copper (Cu). The mean grain yield of three years decreased by 12.1% in SR than that in TP treatment. At maturity stage of winter wheat, soil total N and available Cu was increased by 5.8% in 0-20 cm and 6.2% in 20-40 cm layer, respectively, while soil available P and Mn were decreased by 36.1% and 10.2%, respectively. No difference was observed between SR and TP treatments for soil organic matter, NO-N, available K, S, Zn and Fe at anthesis and maturity stages. Compared to the TP treatment, the mean grain yield was decreased by 12.1% in GM treatment. Soil pH, available P and S were decreased, while the soil organic matter, total N, NO-N, and available Zn and Mn were increased. No difference was observed for soil available K, Fe and Cu. In conclusion, the PM and SR were not beneficial for the improvement of soil fertility, and thus inhibited the grain yield increase in dryland with low soil fertility level. The GM has greater potential to increase soil nutrients, but it should be paid more attention to the risk of grain yield reduction due to insufficient annual precipitation.
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http://dx.doi.org/10.13287/j.1001-9332.201902.019DOI Listing
February 2019

FGL2 prothrombinase contributes to the early stage of coronary microvascular obstruction through a fibrin-dependent pathway.

Int J Cardiol 2019 Jan 13;274:27-34. Epub 2018 Sep 13.

Department of Geriatrics, Institute of Geriatrics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. Electronic address:

Background: Membrane-associated fibrinogen-like protein 2 (FGL2 prothrombinase, pFGL2) is abundantly expressed in activated microvascular endothelial cells (MVECs) and plays a crucial role in microthrombus formation in microcirculatory vasculature. It has been widely reported that coronary microvascular obstruction (CMVO) contributes to adverse outcomes following myocardial ischemia/reperfusion. However, the role of pFGL2 in CMVO is poorly understood.

Methods And Results: We aimed to identify the effect of MVECs-pFGL2 in CMVO using FGL2 knockout mice. As results, the MVECs-pFGL2 expression progresses significantly over 3 days and then gradually decreases, which is positively correlated with the extent of CMVO as detected by HE staining in wild type mice. Furthermore, FGL2 deficiency is correlated with decreased areas of no-reflow and necrosis as detected by Evans Blue and TTC staining and that it ameliorates cardiac dysfunction detected by hemodynamics in the early stage of CMVO. Moreover, fibrin deposition in microvasculature is significantly reduced in FGL2-deficient mice as evidenced by immunohistochemistry, MSB and Carstairs staining, along with the down-regulation of leukocyte adhesion and infiltration. Additionally, we observed that the FGL2 deficiency decreases macrophage infiltration and shifts the macrophage phenotype from pro-inflammatory (M1,) to anti-inflammatory (M2,) pattern in the early stage of CMVO.

Conclusion: These findings highlight the MVECs-pFGL2-fibrin pathway in the early stage of CMVO and provide insights into coagulation and inflammation for the coronary artery disease therapeutics.
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http://dx.doi.org/10.1016/j.ijcard.2018.09.051DOI Listing
January 2019

[Optimum nitrogen application rate to maximum yield and environment protection for winter wheat in Weibei dryland, China.]

Ying Yong Sheng Tai Xue Bao 2018 Aug;29(8):2509-2516

College of Natural Resources and Environment, Northwest A&F University, Yangling 712100, Shaanxi, China.

To get the optimum nitrogen (N) fertilization rate which could guarantee wheat yield and protect environment, we examined wheat yield, N use efficiency, apparent N loss and soil N balance in Weibei dryland with a 3-year field experiment. The results showed that annual wheat yield increased and then decreased as N application rate increased in all the years with different annual rainfall, but the cumulative apparent N use efficiency significantly decreased. Higher yield and N use efficiency were obtained at the fertilization rate of 150 kg·hm. Residual nitrate-N concentrations significantly increased with the increases of N application rate. When the N application rate was between 75 and 150 kg·hm, the apparent N loss and loss rate were nearly the same, but if N application rate was higher than 150 kg·hm, the apparent loss and loss rate significantly increased. In conclusion, N application rate at 150 kg·hm in Weibei dryland could guarantee high yield and N use efficiency, and simultaneously maintain residual nitrate-N concentration and reduce apparent N loss.
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http://dx.doi.org/10.13287/j.1001-9332.201808.024DOI Listing
August 2018

[Effects of Simulated Precipitation Reduction on Soil Respiration in a Soybean-Winter Wheat Rotation Cropland].

Huan Jing Ke Xue 2018 Apr;39(4):1943-1951

School of Applied Meteorology, Nanjing University of Information Science and Technology, Nanjing 210044, China.

To investigate the effects of precipitation reduction on soil respiration in rainfed croplands, a field experiment was performed in a soybean-winter wheat cropland. A randomized block design including three treatments, viz. control (CK), 20% precipitation reduction (P20%), and 40% precipitation reduction (P40%), was used. Seasonal variabilities in soil respiration, soil temperature, and soil moisture were measured. Rates of soil CO production, nitrification and denitrification, and harvested crop biomass were also measured. Results indicated that the seasonal mean soil respiration rates for CK, P20%, and P40% treatments in the soybean growing season were (4.91±0.67), (4.19±0.39), and (4.35±0.32) μmol·(m·s), respectively. There was no significant difference (>0.05) in the mean soil respiration rates between treatments during the soybean growing season. The seasonal mean soil respiration rates for CK, P20%, and P40% treatments during the winter wheat growing season were (2.39±0.17), (2.03±0.02), and (1.94±0.05) μmol·(m·s), respectively. There was a significant (<0.05) difference in the mean soil respiration rates between treatments during the winter wheat growing season. Precipitation reduction decreased the soil CO production rates, but had no obvious impacts on soil nitrification and denitrification rates. Precipitation reduction had no significant (>0.05) effects on the root, shoot, and seed biomass of soybean, but significantly (<0.05) decreased the root, shoot, and seed biomass of winter wheat. Soil temperature was the main driver of the seasonal variation in soil respiration. Soil respiration increased exponentially with the increase in soil temperature. There was no significant (>0.05) difference in the coefficient of temperature sensitivity () between different treatments. Based on the precipitation reduction experiments of duration longer than one year in previous studies and in our present study, a significant linear regression relationship between the amount of reduced soil respiration and the amount of precipitation reduction was found, indicating that substantial precipitation reduction showed more obvious inhibition effects on soil respiration. This study also suggested that the effects of precipitation reduction on soil respiration varied between crop growing seasons, which may be attributed to the different precipitation intensities in different growing seasons.
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http://dx.doi.org/10.13227/j.hjkx.201709009DOI Listing
April 2018

[Effects of nitrogen application on decomposition and nutrient release of returned maize straw in Guanzhong Plain, Northwest China].

Ying Yong Sheng Tai Xue Bao 2017 Jul;28(7):2261-2268

Key Laboratory of Plant Nutrition and Agro-environment in Northwest China, Ministry of Agriculture/College of Natural Resources and Environment, Northwest A&F University, Yangling 712100, Shaanxi, China.

With N isotope labeled maize straw in nylon net bags and buried in the wheat field at two N rates of 0 and 200 kg N·hm, the effects of nitrogen application on the decomposition of straw dry matter and the release dynamics of carbon, nitrogen, phosphorus and potassium (C, N, P and K) after maize straw retention were investigated in the winter wheat-summer maize rotation system in Guanzhong Plain, Shaanxi, China. Results showed that N application did not affect the decomposition of the returned straw C and dry matter, but promoted the release of P and inhibited the release of N and K from straw during sowing to wintering periods of winter wheat. From the grain filling to the harvest of winter wheat, the decomposition of the returned straw and the release of N, P and K were not affected, but the release of straw C was significantly enhanced by N application. The release dynamic of straw C was synchronized with the decomposition of the dry matter, and the C/N of straw declined gradually with the extension of wheat growing. Until the harvest of winter wheat, the accumulative decomposition rate of straw dry matter was less than 50%, and the total straw C release rate was around 47.9% to 51.1%. The C/N ratio of the returned straw was decreased from 32.2 to 20.2 and 17.9, respectively at N rates of 0 and 200 kg N·hm. From sowing to harvest of winter wheat, the net release of N, P and K from the straw was observed. The N release was 7.2-9.4 kg·hm and 12.7%-16.6% of the total straw N, and the P release was 1.29-1.44 kg·hm and 29.0%-32.4% of the total straw P, while a great deal of K was released quickly, with approximately 80% of the straw K released before wintering, 51.8-52.5 kg·hm and 90.5%-91.7% of the total straw K released at wheat harvest. It was suggested that the K fertilizer application should be decreased for the winter wheat due to the great amount K release from the returned maize straw, and an extra amount of N and P fertilizer should be applied under the straw retention cropping system.
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http://dx.doi.org/10.13287/j.1001-9332.201707.010DOI Listing
July 2017

[Soil moisture variation under different water and fertilization managements in apple orchard of Weibei dryland, China].

Ying Yong Sheng Tai Xue Bao 2018 Apr;29(4):1249-1258

College of Natural Resources and Environment,Northwest A&F University/Ministry of Agriculture Key Laboratory of Plant Nutrition and Agri-environment of Northwest, Yangling 712100, Shaanxi, China.

To evaluate the variations of soil moisture under different water and fertilizer treatments in apple orchard in the Weibei dryland, a field experiment was carried out in 2013-2016 at Tianjiawa Village, Baishui County, Shaanxi Province. There were three treatments, i.e., farmers traditional model (only addition of NPK chemical fertilizer, FM), extension model (swine manure and NPK chemical fertilizer combined with black plastic film in tree row space, EM), and optimized model (swine manure and NPK chemical fertilizer combined with black plastic film in tree row space and planting rape in the inter-row of apple trees, OM). The results showed that OM treatment significantly increased soil water storage capacity in 0-200 cm soil layer. Water content of 0-100 cm soil layer was increased by 5.6% and 15.3% in the dry season compared with FM and EM treatment, respectively. Moreover, the soil water relative deficit index of OM was lower than that of EM in 200-300 cm soil layer. The rainfall infiltration in the dry year could reach 300 cm depth under OM. Meanwhile, OM stabilized soil water content and efficiently alleviated the desiccation in deep soil layer. Compared with FM and EM, the 4-year average yield of OM was increased by 36.6% and 22.5%, respectively. In summary, OM could increase water use efficiency through increasing the contents of available soil water and improving the soil water condition in shallow and deep layers, which help alleviate the soil deficit in deep layer and increase yield.
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http://dx.doi.org/10.13287/j.1001-9332.201804.027DOI Listing
April 2018

[Effects of combined application of chemical fertilizer and organic manure on wheat yield and leaching of residual nitrate-N in dryland soil].

Ying Yong Sheng Tai Xue Bao 2018 Apr;29(4):1240-1248

College of Natural Resources and Environment, Northwest A&F University, Yangling 712100, Shaanxi, China.

The effects of optimum nitrogen (N) fertilization rate with and without adding manure on wheat yield and leaching of residual nitrate-N in soil profile were examined in Weibei dryland, Shaanxi with a field experiment combined different N fertilization rates (0, 75, 150, 225, 300 kg N·hm) and organic manure (0 and 30 t·hm). The results showed that, compared to chemical N fertilizer, combined application of inorganic fertilizer and organic manure increased winter wheat yield by 14.7% when N fertilization rate was reduced by 27.1%. The highest yield was obtained when 150 kg·hm of N rate was combined with the manure (N+M). The combination of N fertilizer and manure promoted N uptake of wheat grain and increased N use efficiency by 20.2%. The highest N use efficiency was recorded in the N+M treatment. In addition, the lea-ching of residual nitrate-N during the wheat growing season and the leaching of nitrate-N during summer fallow were decreased. When N application rate was lower than 115 kg·hm, N fertilizer combined with organic manure reduced the amount of nitrate-N leaching in summer fallow. We recommend the combined application of organic manure with about 150 kg·hm of N fertilizers in Weibei dryland to guarantee high winter wheat yield, N use efficiency, and reduce excessive residue of fertilizer N in the soil.
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http://dx.doi.org/10.13287/j.1001-9332.201804.023DOI Listing
April 2018

[Effects of cultivation patterns on wheat yield and grain nutrient concentration in dryland].

Ying Yong Sheng Tai Xue Bao 2018 Mar;29(3):818-826

College of Natural Resources and Environment, Northwest A&F University/Key Laboratory of Plant Nutrition and Agro-environment in Northwest China, Ministry of Agriculture, Yangling 712100, Shaanxi, China.

A field experiment was conducted to examine the effects of plastic film mulching (PM), straw retention (SR) and planting green manure (GM) on grain yield and nutrient concentrations of winter wheat. Compared to the traditional pattern (TP), plastic film mulching showed no significant effect on the average yield over the three years but increased the average phosphorus (P) uptake and concentration in grain by 8.4% and 13.0%, respectively. The average uptake of nitrogen (N), sulfur (S) and iron (Fe) was decreased by 12.6%, 15.0% and 11.1%, and the corresponding concentration was decreased by 12.1%, 12.9% and 10.1%, respectively. There was no significant effect on grain zinc (Zn) concentration. Straw retention decreased grain yield by 12.1%, reduced the average uptake of N, S and Fe decreased by 22.5%, 21.0% and 19.8%, and their corresponding concentration by 10.1%, 9.4% and 3.8%, respectively. The average uptake of P in grain was decreased by 9.8% with straw retention, while the P concentration was increased by 5.0%. There was no significant effect of straw retention on Zn concentration in grain. Planting green manure decreased the grain yield by 12.1%. It had no significant effect on the average uptake of N and Zn, but increased the grain N and Zn concentration by 12.1% and 12.6%, respectively. It showed no impact on P, S and Fe concentration in grain. The discordance between variation of grain yield and its nutrient uptake under different cultivations was the key reason for the changes of their nutrient concentration. Considering the potential adverse effects of plastic film mulching and straw retention on the quantity and quality of grain yield, suitable N fertilization should be applied to ensure the nutrient requirement for grain yield and regulate the uptake and utilization of N, S and Fe for improving the grain quality. Planting green manure could improve soil fertility and increase grain N and Zn concentration, but the yield reduction deserves more attention.
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http://dx.doi.org/10.13287/j.1001-9332.201803.027DOI Listing
March 2018
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