Publications by authors named "Zhao Yang"

3,241 Publications

  • Page 1 of 1

Comparative transcriptomics reveals circadian and pluripotency networks as two pillars of longevity regulation.

Cell Metab 2022 May 10. Epub 2022 May 10.

Department of Biology, University of Rochester, Rochester, NY 14627, USA. Electronic address:

Mammals differ more than 100-fold in maximum lifespan. Here, we conducted comparative transcriptomics on 26 species with diverse lifespans. We identified thousands of genes with expression levels negatively or positively correlated with a species' maximum lifespan (Neg- or Pos-MLS genes). Neg-MLS genes are primarily involved in energy metabolism and inflammation. Pos-MLS genes show enrichment in DNA repair, microtubule organization, and RNA transport. Expression of Neg- and Pos-MLS genes is modulated by interventions, including mTOR and PI3K inhibition. Regulatory networks analysis showed that Neg-MLS genes are under circadian regulation possibly to avoid persistent high expression, whereas Pos-MLS genes are targets of master pluripotency regulators OCT4 and NANOG and are upregulated during somatic cell reprogramming. Pos-MLS genes are highly expressed during embryogenesis but significantly downregulated after birth. This work provides targets for anti-aging interventions by defining pathways correlating with longevity across mammals and uncovering circadian and pluripotency networks as central regulators of longevity.
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http://dx.doi.org/10.1016/j.cmet.2022.04.011DOI Listing
May 2022

MetaMP: Metalearning-Based Multipatch Image Aesthetics Assessment.

IEEE Trans Cybern 2022 May 17;PP. Epub 2022 May 17.

Image aesthetics assessment (IAA) is a subjective and complex task. The aesthetics of different themes vary greatly in content and aesthetic results, whether they are in the same aesthetic community or not. In aesthetic evaluation tasks, the pretrained network with direct fine-tune may not be able to quickly adapt to tasks on various themes. This article introduces a metalearning-based multipatch (MetaMP) IAA method to adapt to various thematic tasks quickly. The network is trained based on metalearning to obtain content-oriented aesthetic expression. In addition, we design a complete-information patch selection scheme and a multipatch (MP) network to make the fine details fit the overall impression. Experimental results demonstrate the superiority of the proposed method in comparison with the state-of-the-art models based on aesthetic visual analysis (AVA) benchmark datasets. In addition, the evaluation of the dataset shows the effectiveness of our metalearning training model, which not only improves MetaMP assessment accuracy but also provides valuable guidance for network initialization of IAA.
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http://dx.doi.org/10.1109/TCYB.2022.3169017DOI Listing
May 2022

Left Heart Chamber Volumetric Assessment by Automated Three-Dimensional Echocardiography in Heart Transplant Recipients.

Front Cardiovasc Med 2022 27;9:877051. Epub 2022 Apr 27.

Department of Ultrasound Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Background: Recently, a new automated software (Heart Model) was developed to obtain three-dimensional (3D) left heart chamber volumes. The aim of this study was to verify the feasibility and accuracy of the automated 3D echocardiographic algorithm in heart transplant (HTx) patients. Conventional manual 3D transthoracic echocardiographic (TTE) tracings and cardiac magnetic resonance (CMR) images were used as a reference for comparison.

Methods: This study enrolled 103 healthy HTx patients prospectively. In protocol 1, left ventricular end-diastolic volume (LVEDV), LV end-systolic volume (LVESV), left atrial max volume (LAVmax), LA minimum volume (LAVmin) and LV ejection fraction (LVEF) were obtained using the automated 3D echocardiography (3DE) and compared with corresponding values obtained through the manual 3DE. In protocol 2, 28 patients' automated 3DE measurements were compared with CMR reference values. The impacts of contour edit and surgical technique were also tested.

Results: Heart Model was feasible in 97.1% of the data sets. In protocol 1, there was strong correlation between 3DE and manual 3DE for all the parameters ( = 0.77 to 0.96, p<0.01). Compared to values obtained through manual measurements, LV volumes and LVEF were overestimated by the automated algorithm and LA volumes were underestimated. All the biases were small except for that of LAVmin. After contour adjustment, the biases reduced and all the limits of agreement were clinically acceptable. In protocol 2, the correlations for LV and LA volumes were strong between automated 3DE with contour edit and CMR ( = 0.74 to 0.93, p<0.01) but correlation for LVEF remained moderate ( = 0.65, < 0.01). Automated 3DE overestimated LV volumes but underestimated LVEF and LA volumes compared with CMR. The limits of agreement were clinically acceptable only for LVEDV and LAVmax.

Conclusion: Simultaneous quantification of left heart volumes and LVEF with the automated Heart Model program is rapid, feasible and to a great degree it is accurate in HTx recipients. Nevertheless, only LVEDV and LAVmax measured by automated 3DE with contour edit seem applicable for clinical practice when compared with CMR. Automated 3DE for HTx recipients is a worthy attempt, though further verification and optimization are needed.
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http://dx.doi.org/10.3389/fcvm.2022.877051DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9091562PMC
April 2022

Mapping the Research on Health Policy and Services in the Last Decade (2009-2018): A Bibliometric Analysis.

Front Public Health 2022 27;10:773668. Epub 2022 Apr 27.

School of Public Health and Management, Binzhou Medical University, Yantai, China.

Background: Health policy and services is a continuously evolving field of research that can inform prevention and control efforts for a variety of health conditions. The "Healthy China" strategy reflects the demand to formulate health policy that suits China's national needs and goals. Applying bibliometric analysis to grasp the general situation of health policy and services research globally will be conducive to informing China's designated health plans and initiatives.

Method: A bibliometric analysis of 58,065 articles on "Health Policy and Services" topics was conducted. The document type was restricted to journal articles that were published in the Web of Science database between the time parameter of January 1, 2009 to December 31, 2018. Data was collected on indicators such as the annual number of publications in the field of health policy and services, the country where the publication is issued, the publication organization, the source journal, the frequency of citations, research hotspots, and academic areas.

Results: The overall number of articles published in Web of Science on health policy and services research has increased over time. The United States has the largest number of articles in the field. The institution with the highest number of citations in the field is Harvard University and the journal with the most published articles in the field is Health Affairs. Research hotspots in the health policy and services field include topics such as "HIV Infections," "Primary Health Care," "Delivery of Health Care," and "Health Services Accessibility."

Conclusion: Experts in the field of health policy and services globally are dedicated to researching the most effective ways to improve people's health and living standards. There is a certain gap in the depth of health policy and services research between China and developed countries and regions such as Europe or America. China must learn from foreign experience to conduct meaningful and informative research that can aid in the formulation of multi-dimensional health policies in specific areas such as environmental infectious diseases, where attention is needed in areas beyond the medical and health system.
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http://dx.doi.org/10.3389/fpubh.2022.773668DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9092023PMC
April 2022

Locally secreted BiTEs complement CAR T cells by enhancing killing of antigen heterogeneous solid tumors.

Mol Ther 2022 May 14. Epub 2022 May 14.

Center for Cellular Immunotherapies, University of Pennsylvania Philadelphia, PA, 19104, USA; GBM Translational Center of Excellence, Abramson Cancer Center, University of Pennsylvania Philadelphia, PA, 19104, USA; Department of Neurosurgery, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA. Electronic address:

Bispecific T-cell engagers (BiTEs) are bispecific antibodies that redirect T cells to target antigen-expressing tumors. We hypothesized that BiTE-secreting T cells could be a valuable therapy in solid tumors, with distinct properties in mono- or multi-valent strategies incorporating chimeric antigen receptor (CAR) T cells. Glioblastomas represent a good model for solid tumor heterogeneity, representing a significant therapeutic challenge. We detected expression of tumor-associated epidermal growth factor receptor (EGFR), EGFR variant III (EGFRvIII), and interleukin-13 receptor alpha 2 (IL13Rα2) on glioma tissues and cancer stem cells. These antigens formed the basis of a multivalent approach, using a conformation-specific tumor-related EGFR targeting antibody (806) and Hu08, an IL13Rα2-targeting antibody, as the scFvs to generate new BiTE molecules. Compared with CAR T cells, BiTE T cells demonstrated prominent activation, cytokine production, and cytotoxicity in response to target-positive gliomas. Superior response activity was also demonstrated in BiTE secreting bivalent T cells compared with bivalent CAR T cells in a glioma mouse model at early phase, but not in the long-term. In summary, BiTEs secreted by mono- or multi- valent T cells have potent anti-tumor activity in vitro and in vivo with significant sensitivity and specificity, demonstrating a promising strategy in solid tumor therapy.
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http://dx.doi.org/10.1016/j.ymthe.2022.05.011DOI Listing
May 2022

NSrp70 suppresses metastasis in triple-negative breast cancer by modulating Numb/TβR1/EMT axis.

Oncogene 2022 May 14. Epub 2022 May 14.

Department of Breast Surgery, Key Laboratory of Breast Cancer in Shanghai, Fudan University Shanghai Cancer Center, Shanghai, 200032, China.

Alternative splicing of mRNA precursors allows cancer cells to create different protein isoforms that promote growth and survival. Compared to normal cells, cancer cells frequently exhibit a higher diversity of their transcriptomes. A comprehensive understanding of splicing regulation is required to correct the splicing alterations for the future precision oncology. A quantitative proteomic screen was performed to identify the regulators associated the metastasis in triple-negative breast cancer. Multiple in vitro and in vivo functional analyses were used to study the effects of NSrp70 on breast cancer metastasis. Next, transcriptomic sequencing (RNA-seq) and alternative splicing bioinformatics analysis was applied to screen the potential targets of NSrp70. Moreover, in vitro splicing assays, RNA pull-down, and RNA immunoprecipitation assay were used to confirm the specific binding between NSrp70 and downstream target genes. Furthermore, the prognostic value of NSrp70 was analyzed in a cohort of patients by performing IHC. We uncovered NSrp70 as a novel suppressor of breast cancer metastasis. We discovered that NSrp70 inhibited the skipped exon alternative splicing of NUMB, promoted the degradation of transforming growth factor receptor 1 through lysosome pathway, and regulated TGFβ/SMAD-mediated epithelial-mesenchymal transition phenotype in breast cancer cells. Furthermore, high NSrp70 expression correlated with a better prognosis in breast cancer patients. Our findings revealed that splicing regulator NSrp70 serves as a metastasis suppressor.
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http://dx.doi.org/10.1038/s41388-022-02349-zDOI Listing
May 2022

A 90-Day Prognostic Model Based on the Early Brain Injury Indicators after Aneurysmal Subarachnoid Hemorrhage: the TAPS Score.

Transl Stroke Res 2022 May 14. Epub 2022 May 14.

Department of Neurosurgery, Fengtai District, Beijing Tiantan Hospital, Capital Medical University, No. 119 South Fourth Ring West Road, Beijing, 100070, China.

This study aimed to establish a new scoring model based on the early brain injury (EBI) indicators to predict the 90-day functional outcomes in patients with aneurysmal subarachnoid hemorrhage (aSAH). We retrospectively enrolled 825 patients and prospectively enrolled 108 patients with aSAH who underwent surgical clipping or endovascular coiling (derivation cohort = 640; validation cohort = 185; prospective cohort = 108) in our institute. We established a logistic regression model based on independent risk factors associated with 90-day unfavorable outcomes. The discrimination of the prognostic model was assessed by the area under the curve in a receiver operating characteristic curve analysis. The Hosmer-Lemeshow goodness-of-fit test and a calibration plot were used to evaluate the calibration of the prediction model. The developed scoring model named "TAPS" (total score, 0-7 points) included the following admission variables: age > 55 years old, WFNS grade of 4-5, mFS grade of 3-4, Graeb score of 5-12, white blood cell count > 11.28 × 10/L, and surgical clipping. The model showed good discrimination with the area under the curve in the derivation, validation, and prospective cohorts which were 0.816 (p < 0.001, 95%CI = 0.77-0.86), 0.810 (p < 0.001, 95%CI = 0.73-0.90), and 0.803 (p < 0.001, 95%CI = 0.70-0.91), respectively. The model also demonstrated good calibration (Hosmer-Lemeshow goodness-of-fit test: X = 1.75, df = 8, p = 0.988). Compared with other predictive models, TAPS is an easy handle tool for predicting the 90-day unfavorable outcomes of aSAH patients, which can help clinicians better understand the concept of EBI and quickly identify those patients at risk of poor prognosis, providing more positive treatment strategies. Trial registration: NCT04785976. Registered 5 March 2021-retrospectively registered, http://www.clinicaltrials.gov .
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http://dx.doi.org/10.1007/s12975-022-01033-4DOI Listing
May 2022

Research Progress in the Field of Gambogic Acid and Its Derivatives as Antineoplastic Drugs.

Molecules 2022 May 4;27(9). Epub 2022 May 4.

Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin 150040, China.

Gambogic acid (GA) is a natural product with a wide range of pharmacological properties. It plays an important role in inhibiting tumor growth. A large number of GA derivatives have been designed and prepared to improve its shortcomings, such as poor water solubility, low bioavailability, poor stability, and adverse drug effects. So far, GA has been utilized to develop a variety of active derivatives with improved water solubility and bioavailability through structural modification. This article summarized the progress in pharmaceutical chemistry of GA derivatives to provide a reference and basis for further study on structural modifications of GA and expansion of its clinical applications.
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http://dx.doi.org/10.3390/molecules27092937DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9102264PMC
May 2022

Comparative Transcriptomics Reveals the Molecular Mechanism of the Parental Lines of Maize Hybrid An'nong876 in Response to Salt Stress.

Int J Mol Sci 2022 May 7;23(9). Epub 2022 May 7.

National Engineering Laboratory of Crop Stress Resistance Breeding, School of Life Sciences, Anhui Agricultural University, Hefei 230036, China.

Maize ( L.) is an essential food crop worldwide, but it is highly susceptible to salt stress, especially at the seedling stage. In this study, we conducted physiological and comparative transcriptome analyses of seedlings of maize inbred lines An'nong876 paternal (cmh15) and An'nong876 maternal (CM37) under salt stress. The cmh15 seedlings were more salt-tolerant and had higher relative water content, lower electrolyte leakage, and lower malondialdehyde levels in the leaves than CM37. We identified 2559 upregulated and 1770 downregulated genes between salt-treated CM37 and the controls, and 2757 upregulated and 2634 downregulated genes between salt-treated cmh15 and the controls by RNA sequencing analysis. Gene ontology functional enrichment analysis of the differentially expressed genes showed that photosynthesis-related and oxidation-reduction processes were deeply involved in the responses of cmh15 and CM37 to salt stress. We also found differences in the hormone signaling pathway transduction and regulation patterns of transcription factors encoded by the differentially expressed genes in both cmh15 and CM37 under salt stress. Together, our findings provide insights into the molecular networks that mediate salt stress tolerance of maize at the seedling stage.
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http://dx.doi.org/10.3390/ijms23095231DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100555PMC
May 2022

Isolation of Potato Endophytes and Screening of Antimicrobial Genes.

Int J Mol Sci 2022 Apr 21;23(9). Epub 2022 Apr 21.

Department of Plant Pathology, College of Plant Science and Technology and the Key Lab of Crop Disease Monitoring & Safety Control in Hubei Province, Huazhong Agricultural University, Wuhan 430070, China.

Antimicrobial peptides (AMPs) have natural antibacterial activities that pathogens find difficult to overcome. As a result of this occurrence, AMPs can act as an important substitute against the microbial resistance. In this study, we used plate confrontation tests to screen out 20 potential endophytes from potato tubers. Among them, endophyte F5 was found to significantly inhibit the growth of five different pathogenic fungi. Following that, phylogenetic analysis revealed that the internal transcribed spacer (ITS) sequences were 99% identical to corresponding sequences. Thereafter, the expression system was used to create a cDNA library in order to isolate the resistance genes. Using this approach, the resistance gene screening technology in the indicator bacteria built-in library was used to identify two antimicrobial peptides, CgR2150 and CgR3101, with broad-spectrum antibacterial activities. Furthermore, the results showed that CgR2150 and CgR3101 have excellent UV, thermal, and enzyme stabilities. Also, these two peptides can significantly inhibit the growth of various bacteria ( pv. oryzae, pv. oryzicola, , and ) and fungi (, , and ). Scanning electron microscopy (SEM) observations revealed that CgR2150 and CgR3101 peptides act against bacteria by disrupting bacterial cell membranes. Moreover, hemolytic activity assay showed that neither of the two peptides exhibited significant hemolytic activity. To conclude, the antimicrobial peptides CgR2150 and CgR3101 are promising in the development of a new antibacterial agent and for application in plant production.
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http://dx.doi.org/10.3390/ijms23094611DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9099842PMC
April 2022

High-resolution detection of quantitative trait loci for seven important yield-related traits in wheat (Triticum aestivum L.) using a high-density SLAF-seq genetic map.

BMC Genom Data 2022 May 13;23(1):37. Epub 2022 May 13.

Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China.

Background: Yield-related traits including thousand grain weight (TGW), grain number per spike (GNS), grain width (GW), grain length (GL), plant height (PH), spike length (SL), and spikelet number per spike (SNS) are greatly associated with grain yield of wheat (Triticum aestivum L.). To detect quantitative trait loci (QTL) associated with them, 193 recombinant inbred lines derived from two elite winter wheat varieties Chuanmai42 and Chuanmai39 were employed to perform QTL mapping in six/eight environments.

Results: A total of 30 QTLs on chromosomes 1A, 1B, 1D, 2A, 2B, 2D, 3A, 4A, 5A, 5B, 6A, 6D, 7A, 7B and 7D were identified. Among them, six major QTLs QTgw.cib-6A.1, QTgw.cib-6A.2, QGw.cib-6A, QGl.cib-3A, QGl.cib-6A, and QSl.cib-2D explaining 5.96-23.75% of the phenotypic variance were detected in multi-environments and showed strong and stable effects on corresponding traits. Three QTL clusters on chromosomes 2D and 6A containing 10 QTLs were also detected, which showed significant pleiotropic effects on multiple traits. Additionally, three Kompetitive Allele Specific PCR (KASP) markers linked with five of these major QTLs were developed. Candidate genes of QTgw.cib-6A.1/QGl.cib-6A and QGl.cib-3A were analyzed based on the spatiotemporal expression patterns, gene annotation, and orthologous search.

Conclusions: Six major QTLs for TGW, GL, GW and SL were detected. Three KASP markers linked with five of these major QTLs were developed. These QTLs and KASP markers will be useful for elucidating the genetic architecture of grain yield and developing new wheat varieties with high and stable yield in wheat.
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http://dx.doi.org/10.1186/s12863-022-01050-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9107147PMC
May 2022

Enhanced trace-amount terahertz vibrational absorption spectroscopy using surface spoof polarization in metasurface structures.

Opt Lett 2022 May;47(10):2446-2449

Terahertz (THz) absorption spectroscopy is a powerful tool for molecular label-free fingerprinting, but it faces a formidable hurdle in enhancing the broadband spectral signals in trace-amount analysis. In this paper, we propose a sensing method based on the geometry scanning of metal metasurfaces with spoof surface polarization sharp resonances by numerical simulation. This scheme shows a significant absorption enhancement factor of about 200 times in an ultra-wide terahertz band to enable the explicit identification of various analytes, such as a trace-amount thin lactose film samples. The proposed method provides a new, to the best of our knowledge, choice for the enhancement of wide terahertz absorption spectra, and paves the way for the detection of trace-amount chemical, organic, or biomedical materials in the terahertz regime.
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http://dx.doi.org/10.1364/OL.452131DOI Listing
May 2022

Assessing the Potential Prognostic and Immunological Role of TK1 in Prostate Cancer.

Front Genet 2022 26;13:778850. Epub 2022 Apr 26.

Department of Urology, Tianjin Institute of Urology, the Second Hospital of Tianjin Medical University, Tianjin, China.

It has been reported that thymidine kinase 1 (TK1) was up-regulated in multiple malignancies and participated in the regulation of tumor malignant behavior. However, its specific role in prostate cancer (PCa) remains unclear. TK1 expression in PCa patients and cell lines was identified via crossover analysis of the public datasets. A series of experiments and models was applied to investigate the function of TK1 in PCa. Functional enrichment analyses were further conducted to explore the underlying mechanism. Additionally, TISIDB was applied to explore the correlation between TK1 expression and tumor-infiltrating lymphocytes, immune subtypes, and immune regulatory factors. TK1 expression was significantly up-regulated in PCa patients and cell lines. TK1 ablation inhibited tumor cell proliferation and migration potential, and experiments showed that TK1 inactivation can significantly restrain tumor growth. Functional enrichment analysis revealed TK1-related hub genes (AURKB, CCNB2, CDC20, CDCA5, CDK1, CENPA, CENPM, KIF2C, NDC80, NUF2, PLK1, SKA1, SPC25, ZWINT), and found that TK1 was closely involved in the regulation of cell cycle. Moreover, elevated mRNA expression of TK1 was related with higher Gleason score, higher clinical stage, higher pathological stage, higher lymph node stage, shorter overall survival, and DFS in PCa patients. Particularly, TK1 represented attenuated expression in C3 PCa and was related with infiltration of CD4, CD8 T cells, and dendritic cells as well as immunomodulator expression. Our study indicates that TK1 is a prognostic predictor correlated with poor outcomes of PCa patients, and for the first time represented that TK1 can promote the progression of PCa. Therefore, TK1 may be a potential diagnostic and prognostic biomarker, as well as a therapeutic target for PCa.
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http://dx.doi.org/10.3389/fgene.2022.778850DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9086852PMC
April 2022

Morphological and dimensional variations of the frontal air sinuses in a group of adolescent Caucasians and Chinese in different skeletal malocclusions: a cross-sectional cephalometric study.

Clin Oral Investig 2022 May 12. Epub 2022 May 12.

School and Hospital of Stomatology, China Medical University, Liaoning Provincial Key Laboratory of Oral Diseases, Shenyang, China.

Objective: This study aimed to evaluate the morphological and dimensional variations of the frontal air sinuses in a group of adolescent Caucasians and Chinese with different skeletal malocclusions in both genders.

Materials And Methods: This retrospective study included 290 adolescent Caucasians and Chinese patients with 145 each. Each sample included 90 females and 55 males and was categorized based on ANB angle in reference to each population norms into 65 skeletal class I, 50 skeletal class II, and 30 skeletal class III malocclusions. All linear, angular, and surface area measurements of the frontal air sinuses were evaluated using lateral cephalometric radiographs and calculated using Winceph version 8 software. The frontal air sinus parameters were compared between genders and the two ethnic groups using an independent sample t-test. ANOVA with Tukey's post hoc tests were used to compare the frontal air sinus parameters between the three skeletal malocclusions.

Result: The frontal air sinus width and surface area were found to be significantly greater in Caucasians when compared with Chinese patients. According to gender, the frontal air sinus length, width, and surface area, as well as the glabella convexity, were greater in males than females, while the frontal air sinus index (length/width) was significantly greater in females than males in both ethnic groups. In both ethnic groups, the frontal air sinus surface area was significantly greater in skeletal class III malocclusion when compared to skeletal class I and class II malocclusions in Caucasians (P = 0.0022) and Chinese (P = 0.0097). There was a weak-to-moderate correlation between the frontal air sinus parameters and the nasio, sella, and glabella positions (R = -0.56 to 0.62).

Conclusion: The frontal air sinus dimensions and surface area varied greatly in between ethnic groups, genders, and malocclusion types. The frontal air sinus parameters were correlated with nasion, sella, and glabella positions.

Clinical Relevance: These findings could assist orthodontists, ENT specialist, and forensic medical investigators to focus on the size of frontal sinus during treatment planning, the relationship between the size of frontal air sinus and malocclusions, and age determination.
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http://dx.doi.org/10.1007/s00784-022-04527-5DOI Listing
May 2022

snoRNAs: functions and mechanisms in biological processes, and roles in tumor pathophysiology.

Cell Death Discov 2022 May 12;8(1):259. Epub 2022 May 12.

Department of Obstetrics and Gynecology, Department of Gynecologic Oncology Research Office, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.

Small nucleolar RNAs (snoRNAs), a type of non-coding RNA, are widely present in the nucleoli of eukaryotic cells and play an important role in rRNA modification. With the recent increase in research on snoRNAs, new evidence has emerged indicating that snoRNAs also participate in tRNA and mRNA modification. Studies suggest that numerous snoRNAs, including tumor-promoting and tumor-suppressing snoRNAs, are not only dysregulated in tumors but also show associations with clinical prognosis. In this review, we summarize the reported functions of snoRNAs and the possible mechanisms underlying their role in tumorigenesis and cancer development to guide the snoRNA-based clinical diagnosis and treatment of cancer in the future.
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http://dx.doi.org/10.1038/s41420-022-01056-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9098889PMC
May 2022

Cyanobacterial Community Structure and Isolates From Representative Hot Springs of Yunnan Province, China Using an Integrative Approach.

Front Microbiol 2022 25;13:872598. Epub 2022 Apr 25.

CAS Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, China.

Cyanobacteria from the representative hot springs of Yunnan Province, China are explored for their diversity and community composition following an integrative approach of cultivation-independent and -dependent studies and further isolation of potential taxa for future biotechnological perspective. 16S rRNA amplicon sequencing of microbial mats in these hot springs with temperature ranging from 38 to 90°C revealed Cyanobacteria and Proteobacteria constituting a bounteous portion of the bacterial community. The combined approach of 16S rRNA amplicon sequencing and phenotypic analysis revealed the diversity of cyanobacteria (a total of 45 genera). Out of these, a total of 19 cyanobacterial taxa belonging to 6 genera and 10 species were isolated as individuals with the possibility of biotechnological utilization. These isolates were subjected to a thorough morphological study and molecular characterization using 16S rRNA gene sequencing for identification and understanding their phylogeny. The identity and phenotypic and genotypic characteristics of 7 cyanobacterial isolates are not identical to any known cyanobacterial species, generating scope for future taxonomic novelties. Preliminary experiments based on high-temperature (50°C) cultivation showed that most of the isolates were thermotolerant and suggested for their high biotechnological usage potential.
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http://dx.doi.org/10.3389/fmicb.2022.872598DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9083006PMC
April 2022

Corrigendum: Microbiome Crosstalk in Immunotherapy and Anti-Angiogenesis Therapy.

Front Immunol 2022 21;13:887775. Epub 2022 Apr 21.

Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medica, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China.

[This corrects the article DOI: 10.3389/fimmu.2021.747914.].
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http://dx.doi.org/10.3389/fimmu.2022.887775DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9070099PMC
April 2022

Association of Egg Consumption with Risk of All-Cause and Cardiovascular Disease Mortality: A Systematic Review and Dose-Response Meta-analysis of Observational Studies.

J Nutr 2022 May 7. Epub 2022 May 7.

Department of Epidemiology and Health Statistics, School of Public Health, Shenzhen University Health Science Center.

Background: Recent studies have reported conflicting associations between egg consumption and the risk of all-cause or cardiovascular disease (CVD) mortality, including ischemic heart disease (IHD) mortality and stroke mortality. With accumulating evidence, up-to-date evidence about the association should be synthesized.

Objective: To assess the association of the risk of all-cause and CVD mortality with egg consumption.

Methods: We searched PubMed, EMBASE, and Web of Science databases through November 3, 2021 for observational studies conducted in participants ≥18 y of age and provided ORs or RRs or HRs and 95% CIs for ≥3 egg consumption categories or for increased intake of egg with the associations of interest. Random-effects model was used to pool the reported risk estimates. Restricted cubic splines were used to examine the dose-response association.

Results: Twenty-four articles with 48 reports (25 for all-cause mortality, 11 for CVD mortality, 6 for IHD mortality, and 6 for stroke mortality) involving 11,890,695 participants were included. Intake of each 1 egg/day increment was associated with increased risk of all-cause mortality (RR 1.06; 95% CI 1.02, 1.10; P = 0.008), but the association was restricted to women, Americans, and studies with adjustments for hyperlipidemia. Egg consumption was linearly associated with CVD mortality only in participants >60 y of age, Americans, studies with follow-up duration≥15 years, and adjustments for hyperlipidemia (P ≤0.018). No significant association was found between egg consumption and IHD or stroke mortality (P ≥0.080).

Conclusions: Egg consumption was linearly associated with a modestly risk of all-cause mortality and, in older participants, Americans, studies with longer follow-up or adjustments for hyperlipidemia, CVD mortality. These findings suggest that it may be prudent to avoid high egg consumption.
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http://dx.doi.org/10.1093/jn/nxac105DOI Listing
May 2022

Dose-response Association of the Dietary Inflammatory Potential With All-Cause and Cause-Specific Mortality.

Adv Nutr 2022 May 7. Epub 2022 May 7.

Department of Epidemiology and Biostatistics, College of Public Health, Zhengzhou University, Zhengzhou, Henan, People's Republic of China.

Although the association of dietary inflammatory potential, evaluated by the dietary inflammatory index (DII), with all-cause and cause-specific mortality has been reported, evidence remains equivocal, no relevant dose-response meta-analysis having been conducted. To examine the dose-response association of dietary inflammatory potential with risk of all-cause, cancer, and cardiovascular disease (CVD) mortality, PubMed, EMBASE and Web of Science were systematically searched up to 9 August 2021. Cohort studies were included if DII was reported as at least three levels or per incremental increase, and if the associations of DII with all-cause, cancer, and CVD mortality were assessed. Generalized least squares regression was used to estimate study-specific dose-response associations, and the random effect model was used to pool the relative risks (RRs) and 95% confidence intervals (95% CIs) of all cause, cancer, and CVD mortality per 1-unit increase in DII. Restricted cubic splines were used to intuitively display the dose-response association between dietary inflammatory potential and mortality. Among the 1,415 studies retrieved, 15 articles (17 cohort studies involving 397,641 participants) were included in this meta-analysis. With per 1-unit increase in DII, the risks were significantly increased for all-cause mortality (RR: 1.04; 95% CI: 1.03, 1.05, I2 = 51.8%; P-heterogeneity = 0.009), cancer mortality (RR: 1.02; 95% CI: 1.00, 1.04, I2 = 58.6%; P-heterogeneity = 0.013), and CVD mortality (RR: 1.04; 95% CI: 1.02, 1.06, I2 = 85.7%; P-heterogeneity <0.001), respectively. Restricted cubic splines showed significant positive linear associations between DII and the above three outcomes. Our study indicated that pro-inflammatory diets may increase the risk of all-cause, cancer, and CVD mortality in a linear manner.
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http://dx.doi.org/10.1093/advances/nmac049DOI Listing
May 2022

Accurate Simulation of Spectroscopic Signatures of Cavity-Assisted, Conical-Intersection-Controlled Singlet Fission Processes.

J Phys Chem Lett 2022 May 6:4280-4288. Epub 2022 May 6.

School of Materials Science and Engineering, Nanyang Technological University, Singapore 639798.

A numerically accurate, fully quantum methodology has been developed for the simulation of the dynamics and nonlinear spectroscopic signals of cavity-assisted, conical-intersection-controlled singlet fission systems. The methodology is capable of handling several molecular systems strongly coupled to the photonic mode of the cavity and treats the intrinsic conical intersection and cavity-induced polaritonic conical intersections in a numerically exact manner. Contributions of higher-lying molecular electronic states are accounted for comprehensively. The intriguing process of cavity-modified fission dynamics, including all of its electronic, vibrational, and photonic degrees of freedom, together with its two-dimensional spectroscopic manifestation, is simulated for two rubrene dimers strongly coupled to the cavity mode.
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http://dx.doi.org/10.1021/acs.jpclett.2c00989DOI Listing
May 2022

Knockdown of HIPK2 attenuates angiotensin II-induced cardiac fibrosis in cardiac fibroblasts.

J Cardiovasc Pharmacol 2022 May 6. Epub 2022 May 6.

Department of Cardiology, Weinan Central Hospital, Weinan 714000, China.

Abstract: Homeodomain-interacting protein kinase-2 (HIPK2), a member of an evolutionary conserved family of serine/threonine kinases, has been observed to be involved in the pathogenesis of fibrotic diseases. However, its role in cardiac fibrosis remains unclear. In the current study, we assessed the effect of HIPK2 on cardiac fibroblasts (CFs) in response to angiotensin II (Ang II) stimulation. The results indicated that HIPK2 expression was significantly increased in Ang II-induced CFs in a dose-dependent manner. Then, HIPK2 was knocked down in CFs to evaluate the roles of HIPK2. Knockdown of HIPK2 suppressed cell proliferation and migration in Ang II-induced CFs. The Ang II-caused increase in expression of α-SMA, a hallmark of myofibroblast differentiation, was decreased by knockdown of HIPK2. HIPK2 knockdown also reduced ECM production including type I collagen (Col I) and connective tissue growth factor (CTGF). Furthermore, knockdown of HIPK2 blocked the activation of TGF-β1/Smad pathway in Ang II-induced CFs. These data suggested that HIPK2 knockdown prevented the Ang II-induced activation of CFs via inhibiting TGF-β1/Smad pathway, indicating HIPK2 might be an anti-fibrosis target for the treatment of cardiac fibrosis.
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http://dx.doi.org/10.1097/FJC.0000000000001292DOI Listing
May 2022

Adenoviral Vector for Enhanced Prostate Cancer Specific Transferrin Conjugated Drug Targeted Therapy.

Nano Lett 2022 May 6. Epub 2022 May 6.

Biochemistry and Molecular Biotechnology, University of Massachusetts Chan Medical School, 364 Plantation Street, LRB 806, Worcester, Massachusetts 01605, United States.

Prostate cancer (PCa) is one of the leading causes of death for men worldwide. Unlike some other types of cancer, there is a lack of targeted therapy for prostate cancer patients that can kill cancer cells but do much less damage to the normal tissue. In this paper, we report on an adenoviral vector enhanced prostate cancer specific transferrin conjugated drug targeted therapy. In particular, a functional PCa-specific gene probe is introduced to drive and up-regulate the transferrin receptor expression on the PCa via adenoviral vector. As a result, significantly enhanced accumulation of nanoscale transferrin-doxorubicin (Tf-DOX) protein drug conjugates and concomitant notably elevated PCa tumor inhibition are observed. This conceptual strategy provides the proof-of-concept for the targeted therapy of PCa that is highly desired but not yet developed.
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http://dx.doi.org/10.1021/acs.nanolett.2c00931DOI Listing
May 2022

Association of Salt-Reduction Knowledge and Behaviors and Salt Intake in Chinese Population.

Front Public Health 2022 18;10:872299. Epub 2022 Apr 18.

Department of Social Medicine and Health Management, School of Public Health, Zhengzhou University, Zhengzhou, China.

Objective: Excessive salt intake is causally associated with an increased risk of cardiovascular disease. Salt-reduction strategies have been rapidly deployed across China since 2017. This study aimed to investigate the association of salt-reduction knowledge and behaviors and salt intake in Chinese population.

Study Design: This study was a national cross-sectional study in China.

Methods: This cross-sectional study was based on data collected during a Chinese adult chronic disease and nutrition surveillance program in 2018 with 7,665 study participants. Salt intake was assessed by calculating 24 h urine sodium from morning urine samples. Logistic regression and mean impact value (MIV) based on the back propagation (BP) artificial neural network were used to screen the potential influencing factors.

Results: A total of 7,665 participants were included in the analysis, with an average age of 54.64 ± 13.26 years, and with men accounting for 42.6%. Only 19.3% of the participants were aware of the Chinese Dietary Guidelines, and only 7.3% of them could accurately identify the level of salt intake recommended in the Chinese Dietary Guidelines. Approximately 41% of the participants adopted salt-reduction behaviors, among whom the number of participants who used less salt when cooking was the highest, and the number of participants who used low sodium salt was the lowest. In the logistic regression, only "No extra salt was added at the table" group showed the effect of salt-reduction, the odds ratio (OR) being 0.78 (95% confidence interval [CI]: 0.64-0.95). The MIV result based on the BP neural network showed that the most important salt-reduction behavior was using less salt when cooking, while reducing eating-out behavior and using salt-limiting tools were the least important.

Conclusion: The research shows that the popularization of salt-reduction knowledge and behaviors can reduce the population's salt intake. However, there is still considerable scope for promoting salt-reduction knowledge and behaviors, while the promotion of salt-reduction tools and low-sodium salt still needs to be strengthened.
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http://dx.doi.org/10.3389/fpubh.2022.872299DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9058069PMC
May 2022

Homogeneous Differentiation of Functional Hepatocytes from Human Induced Pluripotent Stem Cells.

Methods Mol Biol 2022 ;2429:127-142

Division of Toxicology, Office of Applied Research and Safety Assessment, Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, Laurel, MD, USA.

Hepatocyte-like cells (HLCs) generated from human induced pluripotent stem cells (iPSCs) could provide an unlimited source of liver cells for regenerative medicine, disease modeling, drug screening, and toxicology studies. Here we describe a stepwise improved protocol that enables highly efficient, homogeneous, and reproducible differentiation of human iPSCs into functional hepatocytes through controlling all three stages of hepatocyte differentiation, starting from a single cell (non-colony) culture of iPSCs, through homogeneous definitive endoderm induction and highly efficient hepatic specification, and finally arriving at matured HLCs. The final population of cells exhibits morphology closely resembling that of primary human hepatocytes, and expresses specific hepatic markers as evidenced by immunocytochemical staining. More importantly, these HLCs demonstrate key functional characteristics of mature hepatocytes, including major serum protein (e.g., albumin, fibronectin, and alpha-1 antitrypsin) secretion, urea synthesis, glycogen storage, and inducible cytochrome P450 activity.
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http://dx.doi.org/10.1007/978-1-0716-1979-7_9DOI Listing
May 2022

Distinct miRNAs associated with various clinical presentations of SARS-CoV-2 infection.

iScience 2022 May 27;25(5):104309. Epub 2022 Apr 27.

Precision Medicine Center, The First Affiliated Hospital of Xi'an Jiaotong University, 277 Yanta West Road, Xi'an 710061, China.

MicroRNAs (miRNAs) have been shown to play important roles in viral infections, but their associations with SARS-CoV-2 infection remain poorly understood. Here, we detected 85 differentially expressed miRNAs (DE-miRNAs) from 2,336 known and 361 novel miRNAs that were identified in 233 plasma samples from 61 healthy controls and 116 patients with COVID-19 using the high-throughput sequencing and computational analysis. These DE-miRNAs were associated with SASR-CoV-2 infection, disease severity, and viral persistence in the patients with COVID-19, respectively. Gene ontology and KEGG pathway analyses of the DE-miRNAs revealed their connections to viral infections, immune responses, and lung diseases. Finally, we established a machine learning model using the DE-miRNAs between various groups for classification of COVID-19 cases with different clinical presentations. Our findings may help understand the contribution of miRNAs to the pathogenesis of COVID-19 and identify potential biomarkers and molecular targets for diagnosis and treatment of SARS-CoV-2 infection.
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http://dx.doi.org/10.1016/j.isci.2022.104309DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9044631PMC
May 2022

Dynamic trajectory of platelet counts after the first cycle of induction chemotherapy in AML patients.

BMC Cancer 2022 May 1;22(1):477. Epub 2022 May 1.

Center for Big Data Research in Health and Medicine, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Jinan, Shandong, China.

Background: Platelet counts varied over time after induction chemotherapy. We aimed to investigate the different trajectories of platelet counts after the first cycle of induction chemotherapy in patients newly diagnosed with acute myeloid leukemia.

Methods And Results: In total, 149 individuals were included in this study. We identified four distinct trajectories using a group-based trajectory model: low- stability group (n = 27, 18.12%), low-level decrease-medium elevation group (n = 42, 28.19%), low-level decrease-high elevation group (n = 60, 40.27%), and high-level decrease-medium elevation group (n = 20, 13.42%). The baseline characteristics of the high-level decrease-medium elevation group included higher platelet count, lower white blood cell count, lower percentage of bone marrow blasts, and lower rates of complete remission after the first cycle of induction chemotherapy. Compared with the low-stability group, the hazard ratios were 0.32 (95% confidence interval, 0.15-0.68) for the low-level decrease-medium elevation group, 0.31 (95% confidence interval, 0.15-0.63) for the low-level decrease-high elevation group, and 0.35 (95% confidence interval, 0.13-0.89) for the high-level decrease-medium elevation group after adjustment for age and gender by Cox proportional hazard regression. Compared with the low-stability group, the hazard ratios were 0.33 (95% confidence interval, 0.14-0.77) for the low-level decrease-medium elevation group and 0.31 (95% confidence interval, 0.14-0.67) for the low-level decrease-high elevation group after adjustment for age, gender, white blood cell count, and bone marrow blasts. These associations persisted after adjusting for age, gender, white blood cell count, bone marrow blasts, and platelet count.

Conclusion: The dynamic trajectory of platelet counts after the first cycle of induction chemotherapy is a significant predictor of all-cause mortality in patients with acute myeloid leukemia. Timely intervention should be considered for the low-stability group. The low-level decrease-medium elevation and low-level decrease-high elevation groups were independent protective factors for all-cause mortality.
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http://dx.doi.org/10.1186/s12885-022-09601-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9059911PMC
May 2022

A large-scale genome-wide gene-gene interaction study of lung cancer susceptibility in Europeans with a trans-ethnic validation in Asians.

J Thorac Oncol 2022 Apr 29. Epub 2022 Apr 29.

Department of Molecular and Clinical Cancer Medicine, Institute of Translational Medicine, Liverpool University, UK.

Introduction: Although genome-wide association studies have been conducted to investigate genetic variation of lung tumorigenesis, little is known about gene-gene (G×G) interactions that may influence the risk of non-small cell lung cancer (NSCLC).

Methods: Leveraging a total of 445,221 European-descent participants from the International Lung Cancer Consortium OncoArray project, Transdisciplinary Research in Cancer of the Lung and UK Biobank, we performed a large-scale genome-wide G×G interaction study on European NSCLC risk via a series of analyses. First, we used BiForce to evaluate and rank over 58 billion G×G interactions from 340,958 SNPs. Then, the top interactions were further tested by demographically adjusted logistic regression models. Finally, we used the selected interactions to build lung cancer screening models of NSCLC, separately, for never and ever smokers.

Results: With the Bonferroni correction, we identified eight statistically significant pairs of SNPs, which predominantly appeared in the 6p21.32 and 5p15.33 regions (e.g., rs521828 and rs204999, OR = 1.17, P = 6.57×10; rs3135369 and rs2858859, OR = 1.17, P = 2.43×10; rs2858859 and rs9275572, OR = 1.15, P = 2.84×10; rs2853668 and rs62329694, OR = 0.73, P = 2.70×10). Notably, even with much genetic heterogeneity across ethnicities, three pairs of SNPs in the 6p21.32 region identified from the European-ancestry population remained significant among an Asian population from the Nanjing Medical University Global Screening Array project (rs521828 and rs204999, OR = 1.13, P = 0.008; rs3135369 and rs2858859, OR = 1.11, P = 5.23×10; rs3135369 and rs9271300, OR = 0.89, P = 0.006). The interaction empowered polygenetic risk score (iPRS) that integrated classical polygenetic risk score and G×G information score was remarkable in lung cancer risk stratification.

Conclusions: Significant G×G interactions were identified and enriched in the 5p15.33 and 6p21.32 regions, which may enhance lung cancer screening models.
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http://dx.doi.org/10.1016/j.jtho.2022.04.011DOI Listing
April 2022

Engineering Cavity Singlet Fission in Rubrene.

J Phys Chem Lett 2022 May 2;13(18):4090-4097. Epub 2022 May 2.

School of Materials Science and Engineering, Nanyang Technological University, Singapore 639798, Singapore.

By employing the numerically exact multiple Davydov D ansatz, we study cavity-manipulated singlet fission that is mediated by polaritonic conical intersections for both one- and two-molecule systems. The population evolution of the TT state and the cavity photons is carefully examined in search for a high fission efficiency via cavity engineering. Several interesting mechanisms have been uncovered, such as photon-assisted singlet fission, system localization via a displaced photon state, and collective enhancement of the fission efficiency for the two-molecule system. It is also found that the system localization process in the two-molecule system differs substantially from that in the one-molecule system because of the appearance of a novel central polaritonic conical intersection in the two-molecule system. It has been demonstrated that the cavity-controlled singlet fission process can be switched on and off by controlling the average pumping photon number.
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http://dx.doi.org/10.1021/acs.jpclett.2c00801DOI Listing
May 2022

Identification of a Tumor Immunological Phenotype-Related Gene Signature for Predicting Prognosis, Immunotherapy Efficacy, and Drug Candidates in Hepatocellular Carcinoma.

Front Immunol 2022 12;13:862527. Epub 2022 Apr 12.

State Key Laboratory of Southwestern Chinese Medicine Resources, School of Basic Medical Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu, China.

Hepatocellular carcinoma (HCC) is the predominant subtype of primary liver cancer and represents a highly heterogeneous disease, making it hard to predict the prognosis and therapy efficacy. Here, we established a novel tumor immunological phenotype-related gene index (TIPRGPI) consisting of 11 genes by Univariate Cox regression and the least absolute shrinkage and selection operator (LASSO) algorithm to predict HCC prognosis and immunotherapy response. TIPRGPI was validated in multiple datasets and exhibited outstanding performance in predicting the overall survival of HCC. Multivariate analysis verified it as an independent predictor and a TIPRGPI-integrated nomogram was constructed to provide a quantitative tool for clinical practice. Distinct mutation profiles, hallmark pathways, and infiltration of immune cells in tumor microenvironment were shown between the TIPRGPI high and low-risk groups. Notably, significant differences in tumor immunogenicity and tumor immune dysfunction and exclusion (TIDE) were observed between the two risk groups, suggesting a better response to immune checkpoint blockade (ICB) therapy of the low-risk group. Besides, six potential drugs binding to the core target of the TIPRGPI signature were predicted molecular docking. Taken together, our study shows that the proposed TIPRGPI was a reliable signature to predict the risk classification, immunotherapy response, and drugs candidate with potential application in the clinical decision and treatment of HCC. The novel "TIP genes"-guided strategy for predicting the survival and immunotherapy efficacy, we reported here, might be also applied to more cancers other than HCC.
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http://dx.doi.org/10.3389/fimmu.2022.862527DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9039265PMC
May 2022

CT-based radiomics analysis in the prediction of response to neoadjuvant chemotherapy in locally advanced gastric cancer: A dual-center study.

Radiother Oncol 2022 Apr 29;171:155-163. Epub 2022 Apr 29.

Department of Radiology, Shanxi Province Cancer Hospital; Shanxi Hospital Affiliated to Cancer Hospital, Chinese Academy of Medical Sciences; Cancer Hospital Affiliated to Shanxi Medical University; Taiyuan 030013, China. Electronic address:

Background: To investigate the ability of the CT-based radiomics models for pretreatment prediction of the response to neoadjuvant chemotherapy (NAC) in patients with locally advanced gastric cancer (LAGC).

Methods: This retrospective analysis included 279 consecutive LAGC patients from center I (training cohort, n = 196; internal validation cohort, n = 83) who were examined by contrast-enhanced CT before treatment and 211 consecutive patients from center II who were recruited as an external validation cohort. A total of 102 features were extracted from the portal venous phase CT images, and feature selection was further subjected to three-step procedures. Next, five classifications, including Logistic Regression (LR), Naive Bayes, Random forest (RF), Support Vector Machine (SVM), and Extreme Gradient Boosting (XGB) algorithms, were applied to construct radiomics models for predicting the good-responder (GR) to NAC in the training cohort. The prediction performances were evaluated using ROC and decision curve analysis (DCA).

Results: No statistically significant difference was detected for all clinicopathological characteristics. Additionally, allsix key features were significantly different between GR and poor-responder (PR). Compared to models from other classifiers, the model obtained with XGB showed promising prediction performance with the highest AUC of 0.790(95%CI: 0.700-0.880) in the training cohort. The corresponding AUCs were 0.784(95%CI, 0.659-0.908) and 0.803(95%CI, 0.717-0.888) in the internal and external validation cohorts, respectively. DCA confirmed the clinical utility.

Conclusions: The proposed pretreatment CT-based radiomics models revealed good performances in predicting response to NAC and thus may be used to improve clinical treatment in LAGC patients.
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http://dx.doi.org/10.1016/j.radonc.2022.04.023DOI Listing
April 2022
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