Publications by authors named "Zhanquan Yang"

31 Publications

[Research of cellular toxic effect to Hep-2 of recombinant toxin MSH-Ang].

Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2009 Mar;23(5):225-6

Key Laboratory of Otolaryngology Head and Neck Surgery, Ministry of Education, Capital Medical University Tongren Hospital, Beijing, 100730, China.

Objective: To study the cytotoxicity of recombinate toxin MSH-Ang to Hep-2.

Method: The depurated MSH-Ang were applied in cytotoxicity experiment, and the growth inhibiting action to laryngeal carcinoma cell Hep-2 were observed.

Result: Recombination protein inhibited the growth of laryngeal carcinoma cell Hep-2, and its inhibiting action enhanced and corpuscular mortality rate increased along with the concentration increasing.

Conclusion: Recombinant toxin MSH-Ang can not only take special effect in tumors with high MSHR, but also target to many other popular tumors.
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March 2009

[Experience in treating anterior-lateral benign tumor in middle cranial fossa base through standard facial translocation approach ].

Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2007 Apr;21(7):304-5, 308

Department of Otolaryngology-Head and Neck Surgery,China-Japan Union Hospital of Jilin University, ChanMchun, 130031, China.

Objective: To observe the feasibility and effect of improved standard facial translocation approach in treating anterior-lateral benign tumor in middle cranial fossa base.

Method: We excised 10 cases of benign tumor originating from pharynx nasals of pterygopalatine fossa and expanding toward the cavernous sinus of middle cranial fossa, infratemporal fossa or pterygo-maxillary space. We ameliorated, facial incision, maxilla disassembled, reestablishment, fixation,maxillary mucosal option and remained lateral wall of nasal cavity to some extent.

Result: The mean time of following-up was 38 month postoperatively without complications of bleeding, infection and necrosis or prolapse of displacing bones. Nine patients were treated successfully, one died.

Conclusion: Reforming facial translocation approach in treating anterior-lateral benign tumor in middle cranial fossa base was safe and obtained ideal curative effect in near future.
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April 2007

[Expression and distribution of aquaporin 1 in laryngeal carcinoma].

Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2007 Mar;21(6):269-72

Department of Otorhinolaryngology-Head and Neck Surgery, Clinical Hospital, Yangzhou University, Yangzhou, 225001, China.

Objective: To detect expression level and distribution of aquaporin 1 (AQP1) in laryngeal carcinoma tissues and try to find out the significance about the mechanism of AQP1 in the invasion of laryngeal carcinoma.

Method: Twenty cases of laryngeal carcinoma tissues and 15 cases of normal tissues beside tumors were acquired immediately after surgery. The expression and distribution of aquaporin 1 in laryngeal carcinoma were examined by RT-PCR, western blot analysis and immunohistochemical technique.

Result: AQP1 expresses on the capillary endothelia of laryngeal mucous lamina propria and laryngeal mucous glands of normal tissues and mainly on the capillary endothelia cells, tumor cells and cancer nests of laryngeal carcinoma tissues. AQP1 mRNA and protein expression level in laryngeal tumor tissues is remarkably stronger than that in normal tissues beside tumors.

Conclusion: The expression of AQP1 was increased in laryngeal carcinoma tissues over normal laryngeal tissues. It suggests that the mechanism of AQP1 in laryngeal carcinoma etiopathogenesis needs to be studied.
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March 2007

[Application of coblation assisted upper-airway procedure to obstructive sleep apnea-hypopnea syndrome].

Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2007 Feb;21(3):107-9

Department of Otolaryngology-Head and Neck Surgery, China-Japan Union Hospital of Jilin University, Changchun, China.

Objective: To investigate the efficacy and the value of CAUP in treatment of OSAHS.

Method: CAUP and the dissection of palatopharyngeus muscle were performed by RFVTR . One hundred and sixty-eight patients with OSAHS treated by CAUP from July 2001 to July 2004 were summarized. Among them, 52 patients were analyzed by PSG after 1 year of the operation.

Result: All the operative procedure were carried out smoothly with excellent patient tolerance. Post-operation complications were foreign body feeling in oral cavity. No velopalatal insufficiency occurred. The efficient rate was 94.2% after 1 year of the operation according to the PSG results.

Conclusion: CAUP is a simple, safe,repeatable and acceptable surgical procedure and it was developing with excellent value in the treatment of OSAHS on the basic of accurate X-ray imaging.
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February 2007

[Somato-form disorder in otorhinolaryngology-head and neck surgery (4 cases reports)].

Lin Chuang Er Bi Yan Hou Ke Za Zhi 2006 Apr;20(8):339-40

Department of Otorhinolaryngology-Head and Neck Surgery, China-Japan Union Hospital of Jilin University, Changchun, 130033, China.

Objective: To emphasize the significance of the "bio-psycho-society" medical model in clinic practice of otorhinolaryngology-head and neck surgery.

Method: Four cases of patients with somatoform disorder in otorhinolaryngology-head and neck surgery were reported in the paper.

Result: The patients complained that there were some significantly somato symptoms in otorhinolaryngology-head and neck region. The severity of symptoms complained by these patients, however, was not coincidence with the result of careful clinical examination performed by doctors. Noteworthily, there were important psychological factors before in these patients with careful inquiring the history and some somato symptoms were significantly improved after antianxiety treatment.

Conclusion: It is important to think highly of psychological factors in clinical practice.
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April 2006

[Expression of aquaporin 1 and aquaporin 4 in rat laryngeal tissue].

Lin Chuang Er Bi Yan Hou Ke Za Zhi 2006 Feb;20(3):132-4

Department of Otorhinolaryngology-Head and Neck Surgery, China-Japan Union Hospital, Jilin University, Changchun, China.

Objective: To detect expression level and distribution of aquaporin 1(AQP1) and aquaporin 4 (AQP4) in rat laryngeal tissues and try to find out significance about aquaporin in the laryngeal function and mechanism on the laryngeal diseases.

Method: Eight cases laryngeal tissues were obtained from normal wistar rats. The slides were detected with HE staining for routine histopathologic examination, immunohistochemical staining was performed for observing the expression of AQP1 and AQP4 in laryngeal tissue.

Result: AQP1 expresses mainly on the capillary endothelial cell of laryngeal mucous lamina propria, laryngeal mucous glands and myocyte nuclear membrane. AQP4 expresses mainly on squamous epithelial, pseudostratified ciliated columnar epithelium, laryngeal lamina propria mucous glands and myocyte membrane.

Conclusion: The distribution of Aquaporin 1 and aquaporin 4 on rat laryngeal tissue is dramatically different. This result suggests that they may have different role on the water transport of larynx, and may play an important role on laryngeal diseases.
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February 2006

[Effects of hypericin associated with radiotherapy on human laryngeal squamous cell carcinoma strain Hep-2].

Lin Chuang Er Bi Yan Hou Ke Za Zhi 2005 Dec;19(24):1127-30

Department of Otorhinolaryngology-Head and Neck Surgery, Jilin University China-Japan Union Hospital, Changchun.

Objective: To study effects of hypericin associated with radiotherapy on human laryngeal squamous cell carcinoma strain Hep-2.

Method: Using techniques of tumor cells culture in vitro, Hep-2 cells were exposed to different concentration hypericin as 0, 1.0, 2.0, 3.0 microg/ml, then 5 Gy radiation were given after an hour. Other groups Hep-2 culture cells were also exposed to different concentration hypericin as 5, 10, 20, 25 microg/ml, and no radiation were given. In all groups, contrast group was set up. And 48 hours later, growth characteristics of Hep-2 were studied by morphological observation, MTT assay and flow cytometry.

Result: In normal contrast group, Hep-2 cells grew intensively and contacted with each other. And cellular swelling was found when Hep-2 cells were only treated with radiotherapy. When Hep-2 cells were treated with both Hypericin and radiotherapy, more cells swelled and cell number declined greatly. In higher dose Hypericin group, necrosis could be found. MTT assay showed the Hep-2 cells growth was significantly inhibited when treated with both hypericin and radiotherapy. Flow cytometry showed hypericin could block cell growth at G0/G1 phase and induced laryngeal cells into apoptosis whether with radiotherapy or not. But Hep-2 cells only treated with hypericin could not get same powerful effect as combination with radiotherapy.

Conclusion: Human laryngeal squamous cell carcinoma strain Hep-2 can be inhibited and induced into apoptosis by treated with hypericin combination with radiotherapy.
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December 2005

[Anatomy of the extended transsphenoidal approach under endoscopy and its application on treating the sellar and parasellar lesion].

Lin Chuang Er Bi Yan Hou Ke Za Zhi 2005 Jun;19(11):481-3

Department of Otolaryngology-Head and Neck Surgery, Dalian Central Hospital, Dalian, 116033, China.

Objective: The endoscopic surgical anatomy of the extended transsphenoidal approach and its application was studied to establish the anatomic basis for parasellar lesion.

Method: Four adult cadaveric heads were studied by imitating extended transsphenoidal approach and some anatomic parameters were measured.

Result: The sphenoid sinus was divided into five compartments by posterior bony wall. The periosteum, dura, cavernous sinus, carotid artery,ocular nerve, trochlea nerve and adduce nerve crossing cavernous sinus could be visualized. After sphenoidal planum was removed, optic nerve, chiasm and pituitary gland could be displayed. A patient with gigantic pituitary adenoma was cured by extended transsphenoidal approach.

Conclusion: The sellar and parasellar region can be visualized through extended transsphenoidal approach and lesion of sellar turcica can be treated by the approach. Surgeons should be skilled at endoscopic or microscopic sellar anatomy and have experience with transsphenoidal surgery.
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June 2005

Expression of TLR2 and TLR4 messenger RNA in the epithelial cells of the nasal airway.

Am J Rhinol 2005 May-Jun;19(3):236-9

Department of Otorhinolaryngology-Head and Neck Surgery, China-Japan Union Hospital, Bethune Medical School of Jilin University, 126 Xiantai dajie, Changchun, Jilin 130033, China.

Background: Epithelium of nasal mucosa is the first line of defense against invading pathogens. This study investigated the expression of Toll-like receptor (TLR) 2 and TLR4 in epithelial cells of nasal mucosa and understood the role of TLRs in the innate immunity of nasal mucosa.

Methods: Human nasal epithelial cells were obtained by scraping the middle one-third of inferior turbinates from 30 patients with chronic rhinosinusitis and 20 healthy adult volunteers. The epithelial cells are made into smears. In situ hybridization was performed for TLR2 and TLR4 messenger RNA (mRNA).

Results: TLR2 and TLR4 mRNA were expressed in the nasal epithelial cells. The expression of the two genes was significantly higher in the chronic rhinosinusitis group than in the normal control (TLR2, t = 8.605, p < 0.0005; TLR4, t = 9.050, p < 0.0005).

Conclusion: This study is the first to establish the presence of both TLR2 and TLR4 mRNA on epithelial cells of nasal mucosa, and their expression can be up-regulated in infectious conditions. These results show that TLR2 and TLR4 may play a important role in local host defense of nasal mucosa.
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January 2006

[The endoscopic extended transsphenoidal surgery for gigantic pituitary adenoma].

Lin Chuang Er Bi Yan Hou Ke Za Zhi 2005 Jan;19(2):57-9

Department of Otolaryngology-Skull Base Surgery, Xuanwu Hospital, Capital Medical Science University, Beijing, 100053, China.

Objective: The purpose of the study is to evaluate the ability of endoscopic extended transsphenoidalapproach for treatment of gigantic pituitary adenoma.

Method: The clinical data of 13 cases with gigantic pituitary adenoma treated by endoscopic extended transsphenoidalapproach was studied retrospectively.

Result: The tumor of all patients was total resection in endoscopes. Postoperative 10 Patients received radiotherapy. In postoperative MRI examination, there was remnant of tumor in 6 patients. There were no regrowth or recurrence during 12 months postoperative. Six patients occurred transient diabetes insipidus, 2 patients with transient cerebrospinal rhinorrhoea cured by conservative therapy. One patient occurred acute hypopituitarism postoperatively. There were no death or intracranial infection and nasal complication such as nasal adherence in all patients.

Conclusion: The extended transsphenoidalapproach can be used for treatment of gigantic pituitary tumor. The surgeon should be familiarity with the sellar and parasellar anatomy and skilled with transsphenoidal surgery.
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January 2005

[Skull base reconstruction and rehabilitation].

Lin Chuang Er Bi Yan Hou Ke Za Zhi 2004 Dec;18(12):755-7

Department of Otolaryngology-Head and Neck Surgery,Xuanwu Hospital of Capital University of Medical Science, Beijing, 100053, China.

Objective: To review our experience with skull base reconstruction and rehabilitation.

Method: The clinical data of 30 patients with skull base reconstruction from 200 patients performed skull base surgery were retrospectively reviewed. Bone defect of skull base were repaired in 20 patients and dura defect in 6 patients and soft tissue defect in infratemporal fossa in 8 patients.

Result: Postoperatively there were transient cerebrospinal leakage in 3 patients, and non-symptom intracranial pneumatosis in 3 patients, and epidural hematoma in 1 patient, and meningitis in 1 patients.

Conclusion: Immediate reconstruction of skull base with suitable material and surgical technique can prevente complications such as postoperative infection and cerebrospinal leakage brain hernation.
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December 2004

[Clinical analysis of 6 cases with subarachnoid hemorrhage during transsphenoidal surgery for lesions of sellar region].

Lin Chuang Er Bi Yan Hou Ke Za Zhi 2002 Oct;16(10):533-4

Department of Otorhinolaryngology-Head and Neck Surgery, China-Japan Union Hospital of Jilin University, Changchun 130031.

Objective: To learn more about the rare application of subarachnoid hemorrhage (SAH) during transsphenoidal surgery (TSS) for lesions of sellar region.

Method: Clinical statistics of 6 cases with SAH during TSS for lesions of seller region between 1964- 2001 was analyses.

Result: 2 cases died and 4 cases were cured.

Conclusion: The reason of SAH was from the residual tumor (2 cases) and from damaged sellar and arachnoidea. The doctor familiars the more with the anatomy of seller and skill of TSS, the complication decreases the more. Once SAH occurs, comprehensive treatment should be accepted.
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October 2002

[Study on the clearance function of mucociliary system in nasal middle meatus].

Lin Chuang Er Bi Yan Hou Ke Za Zhi 2002 Oct;16(10):530-2

Department of Otorhinolaryngology-Head and Neck Surgery, China-Japan Union Hospital of Jilin University, Changchun 130031.

Objective: To explore the clearance function of mucociliary system in nasal middle meatus.

Method: The nasal middle meatus mucociliary transport rate (MTR) was measured by saccharin method in normal persons and patients with chronic suppurative sinusitis or chronic hypertrophic rhinitis or chronic simple rhinitis. The MTR in nasal common meatus were measured in persons as many as the people mentioned above with the same conditions. Samples of mucosa from nasal middle meatus in normal adults and patients with nasal polyps or chronic sinusitis were scanned by electron microscopy.

Result: in healthy groups, the MTR of nasal middle meatus was (4.47 +/- 0.90) mm/min, and the MTR of nasal common meatus was(7.0 +/- 2.21) mm/min. There was significant difference between them (P < 0.01). The MTR of nasal middle meatus in patients groups were significantly lower than that in healthy groups (P < 0.01). In the patients groups, the MTR of nasal middle meatus were also lower than that of nasal common meatus and that in healthy groups. There were no difference between men and women. The electron microscopy scan showed that the cilias in nasal middle meatus distributed better in healthy persons than in patients. Some short cilias could be found, and the long cilias and the short cilias growthed alternately.

Conclusion: MTR of nasal middle meatus is lower than that of nasal common meatus , so the protective function of mucociliary of nasal middle meatus is lower than that of nasal common meatus.
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October 2002

[Diagnosis and treatment of sever craniofacial compound injure].

Lin Chuang Er Bi Yan Hou Ke Za Zhi 2002 Mar;16(3):106-8

Department of Otolaryngology-Head and Neck Surgery, China-Japan Union Hospital of Jilin University, Changchun 130031.

Objective: It is purpose to analysis the diagnosis and treatment of sever craniofacial compound injure.

Method: The surgical method of reposition of bone fracture of 21 cases with sever craniofacial compound injure included transfacial wound (9 cases) and transmidfacial approach(4 cases) and craniofacial approach (4 cases) and multi incision subperiosteum tunnel approach(4 cases).

Result: In 16 cases which been followed, the function of chew has been recovered.

Conclusion: Sever craniofacial compound injures is a complicate emergention. Doctor must master comprehensive classified method of fracture and ability of diagnosis and attach importance to surgical reposition and technique of rigid internal fixation.
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March 2002

[Ciliogenesis in human nasal epithelial cells cultured at the air-liquid interface].

Lin Chuang Er Bi Yan Hou Ke Za Zhi 2004 Feb;18(2):88-90

Department of Otolaryngology, First Clinical Academy of Medical College of Xiamen University, Xiamen, 361004, China.

Objective: In the present study, our aim was to develop a method to quantitative cell differentiation in cultures of human nasal epithelium (HNE) cells.

Method: HNE cells were cultured on collagengel-coated membranes at an air-liquid interface (ALI) in hormone-and growth factor-supplemented medium. The percentage of the culture surface covered with ciliated cells was estimated using scanning electron microscope and image analysis.

Result: In present study, if an ALI was not established and the cells were maintained in the submerged state, ciliated cell differentiation was pool, the average ciliated surface area was 0.3% when cultures were submerged for fourteen days. When HNE cells cultures in ALI, average ciliated surface area was 8.6%.

Conclusion: Our study demonstrated increased ciliogenesis in ALI.
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February 2004

[The regulation of PKCalpha in eosinophil infiltration and proliferation in nasal polyps].

Lin Chuang Er Bi Yan Hou Ke Za Zhi 2004 Feb;18(2):70-1

Department of Otorhinolaryngology-Head and Neck Surgery, China-Japan Union Hospital, Third Clinical Hospital of Jilin University, Changchun, 130031, China.

Objective: To explore the regulation of protein kinase C (PKC) isoform--PKCalpha in eosinophil (EOS) proliferation and infiltration in nasal polyp tissues.

Method: With the methods of in situ hybridization staining and immunohistochemistry MGG staining, to check out the relationship between PKC and bcl-2/BaxmRNA and associated protein, especially PKC isoform--PKCalpha, PKCbeta1 , PKCbeta2, and PKCgamma did not express at all.

Result: There were PKC expression in the eosinophils of 26 cases from nasal polyps, and the expression of PKC and Bcl-2 mRNA/their protein in EOS of nasal polyps showed remarkably positive relation (r1 = 0.0875, r2 = 0.0823, P < 0.01), but in PKC isoforms, PKCalpha expression was the strongest, but PKCbeta1 and PKCbeta2 expressed thinner and PKCgamma did not express at all.

Conclusion: The reason of eosinophil proliferation and infiltration in nasal polyps is that PKC signal transduction pathway was activated, and leaded to inhibition of eosinophil apoptosis, and eosinophil survival was delayed, and eosinophil proliferated and infiltrated, and in PKC family, PKCalpha is main.
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February 2004

[Expression of inducible nitric oxide synthase mRNA in epithelial cell of nasal mucosa is upregulated through Toll-like receptor-4].

Lin Chuang Er Bi Yan Hou Ke Za Zhi 2004 May;18(5):268-9

Department of Otorhinolaryngology-Head and Neck Surgery, China-Japan Union Hospital of Jilin University, Changchun, 130031, China.

Objective: To investigate the upregulation of inducible nitric oxide synthase (iNOS) in epithelial cell of nasal mucosa through Toll-like receptor-4 (TLR-4).

Method: Specimens from 30 patients of chronic sinusitis and 21 healthy adults were examined by in situ hybridization for TLR-4 and iNOS mRNA.

Result: All 30 samples of chronic sinusitis showed a stronger expression of TLR-4 and iNOS than in controls (P<0.01). iNOS upregulated in nasal epithelium correlated with TLR-4 (r=0.435, P<0.01).

Conclusion: It suggested that TLR-4 may play a role in enhancing local host defence and inflammation via upregulating the expression of iNOS mRNA and the subsequent increased production of NO.
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May 2004

[Expression of human beta-defensin in palatine tonsil].

Lin Chuang Er Bi Yan Hou Ke Za Zhi 2004 Mar;18(3):129-31

Department of Otolaryngology-Head and Neck Surgery, China-Japan Union Hospital of Jilin, University, Changchun, 130031, China.

Objective: To examine the expression of human beta-defensin-1 (hBD-1) and human beta-defensin-2 (hBD-2) in palatine tonsil of normal subjects and patients with chronic tonsillitis, and to investigate the role of palatine tonsil in the innate immunity system.

Method: Eighteen specimens of hyperplastic tonsils (n = 8) and chronic tonsillitis(n = 10) were examined using reverse transcriptase-polymerase chain reaction (RT-PCR).

Result: beta-defensin 1 mRNA was expressed in all tissue specimens, at levels that did not differ significantly (P > 0.05). beta-defensin 2 mRNA was detected in the specimens of chronic tonsillitis, but almost not in the normal subjects. Its expression level was significantly higher in chronic inflamed tonsils than in normal controls(P < 0.05).

Conclusion: These results suggest that beta-defensin of the palatine tonsil may play an important role in innate defense against microorganisms.
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March 2004

[The injury of nasal mucosa in transnasal endoscopic sinus surgery by light and heat].

Lin Chuang Er Bi Yan Hou Ke Za Zhi 2003 Nov;17(11):670-1

Department of Otorhinolaryngology-Head and Neck Surgery, China-Japan Union Hospital, Jilin University, Changchun 130031.

Objective: To investigate the injury of nasal mucosa in transnasal endoscopic sinus surgery by light and heat.

Method: The techniques of transmission electron microscope and scanning electron microscope were applied in order to observe the changes between normal and irradiation nasal septum mucosa of rabbits.

Result: It showed that after irradiation, the cilia became mal-distributed, adhesive and exfoliative. With the elongation of irradiation, the spacing of the epithelium cells increased, the distribution of the cilia was sparse and the degree of their thickness was different, the mitochondria were highly bloated, accompanied with disappearance of the cristae and vacuolation of matrix, the nucleus of epithelium cells was edge-located, the distribution of the chromatin was mass, concentrated by the fringe, the nucleus was close to the cell surface and the exfoliation of goblet cells was visible.

Conclusion: The light and heat in transnasal endoscopic sinus surgery can injure the nasal mucosa.
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November 2003

Protein kinase C in proliferation and infiltration of eosinophils in nasal polyp.

Chin Med J (Engl) 2003 Oct;116(10):1553-6

Department of ORP-HNS, China-Japan Union Hospital, Jilin University, Changchun 130031, China.

Objective: To explore the significance of protein kinase C (PKC) in proliferation and infiltration of eosinophils in nasal polyps.

Methods: With in situ hybridization and immunohistochemistry staining methods, PKC, pro-apoptotic, and anti-apoptotic gene (Bax, bcl-2) expressions were measured in nasal polyp tissues from 26 patients and inferior turbinate mucosa tissues (ITMTs) from 20 healthy persons. The May-Grünwald-Giemsa (MGG) staining method was used to identify eosinophils.

Results: In eosinophils, the positive cell expressive rates of Bcl-2 mRNA and its protein were significantly higher in the group with nasal polyps than in the ITMT group (P < 0.01). Although the positive cell expressive rate of Bax mRNA and associated protein were a little higher in the group with nasal polyp tissues than in the ITMT group, the difference was not significant (P > 0.05). There was PKC expression in the eosinophils of 26 cases of nasal polyps, but occasional PKC expression in 7 of 20 ITMT cases. In the two groups, PKC positive cell expression was significantly different, and the expression of PKC and bcl-2 mRNA as well as associated protein in eosinophils of nasal polyps showed a remarkably positive relationship (r1 = 0.0875, r2 = 0.0823, P < 0.01).

Conclusions: Increased PKC expression in eosinophils of nasal polyp tissues is closely associated with apoptosis inhibition, and it is presumed that eosinophil apoptosis inhibition in nasal polyp tissues is obtained by activation of the PKC signal transduction pathway.
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October 2003

[Avoidance strategies of the surgical treatment of obstructive sleep apnea hypopnea syndrome].

Zhonghua Er Bi Yan Hou Ke Za Zhi 2002 Dec;37(6):422-4

Department of Otorhinolaryngology Head and Neck Surgery, China-Japan Union Hospital, Jilin University, Changchun 130031, China.

Objective: To discuss the avoidance strategies of severe perioperative complications in surgical treatments of obstructive sleep apnea hypopnea syndrome (OSAHS).

Method: 180 cases of uvulopalatopharyngoplasty(UPPP) and 1 case of nose bleeding were analyzed, 10 cases of severe complications reported.

Results: 1 case of Angina pectoris and 1 case of infarction 3 case of nasopharyngeal stenosis, 3 cases pharyngeal bleeding, 1 case of palatal incompetence, 1 case of death occurred postoperatively.

Conclusion: OSAHS is a systemic disease syndrome. The perioperative treatments is very important for the prevention of some severe complications occurrence.
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December 2002

[Study on molecular mechanism of anti-aging effect of rhidosin on olfactory bulb in senile rats].

Lin Chuang Er Bi Yan Hou Ke Za Zhi 2003 Feb;17(2):100-1, 104

Department of Otorhinolaryngology-Head and Neck Surgery, China-Japan Union Hospital, Jilin University, Changchun 130031.

Objective: To research the molecular mechanism of anti-aging effect of rhidosin on olfactory bulb in senile rats.

Method: Ten 3 month-old rats and twenty 26-month-old rats were randomed in rhidosin and saline groups. After decapitated the rats, the olfactory bulb were cut and immediately fixed with neutral formation, followed with paraffin-embedding, serial sectioning, immunohistochemical staining and light microscopic observation.

Result: The proteins of FGF, Bcl-2 and Bax were mainly expressed in mitral cells in the olfactory bulb of the rats. The positive expression rate of FGF and Bcl-2 in young rats group was significantly higher than that in old rats group(P < 0.01). The FGF and Bcl-2 were positive relationship(r = 0.8971; P < 0.01). The expression of Bax in young control group was slightly lower than that in old control group, but there was no significant difference (P > 0.05). The positive expression rate of FGF and Bcl-2 in rhidosin group was significantly higher than that in control group in old rate group(P < 0.01).

Conclusion: FGF is a factor of ascending the anti-apoptosis gene Bcl-2 in mitral cells; rhidosin can increase the expression of FGF and Bcl-2 proteins. We guess that the mechanism of rhidosin anti-aging effect on the olfactory system may be through the effect of increasing the expression of FGF to inhibit the apoptosis of mitral cells.
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February 2003

Local tissue hypoxia and formation of nasal polyps.

Chin Med J (Engl) 2003 Feb;116(2):243-7

Department of Otorhinolaryngology & Head and Neck Surgery, Chinese-Japanese Union Hospital, Bethune Faculty of Medicine, Jilin University, Changchun 130031, China.

Objective: To explore the response of nasal mucosa epithelial cells to hypoxia in terms of formation of nasal polyps (NP).

Methods: Epithelial cells of NP and inferior turbinate (IT) were cultured serum-free under normal oxygen and hypoxic circumstances with stimulation of IL-1 beta and TNF alpha. The vascular endothelial growth factor (VEGF) mRNA and VEGF protein levels of the cultured cells were detected using in situ hybridization and ELISA, respectively.

Results: The expression of VEGF mRNA was significantly higher in epithelial cells of NP than in IT exposed to pro-inflammatory cytokines or hypoxia (P < 0.01). VEGF levels were higher in NP epithelial cells than those of IT (P < 0.01) under hypoxia.

Conclusion: VEGF-induced by hypoxia is very important for the early stages of forming polyps.
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February 2003

[Immunoregulatory effect of Bacillus Calmette Guerin aerosol to allergic airway diseases].

Zhonghua Er Bi Yan Hou Ke Za Zhi 2002 Aug;37(4):267-70

Department of Otorhinolaryngeal-Head Neck Surgery, Chinese-Japan Union Hospital of Jilin University, Changchun 130031, China.

Objective: To investigate whether bacillus calmette guerin (BCG) is effective used transairway and its preventive mechanisms to allergic airway diseases.

Methods: Animal experiment was finished. Adult rats were devided into four groups: control group, ovalbumin-sensitized group, BCG used transairway group, BCG used transairway + ovalbumin-sensitized group. Then these animals symptoms were studied and the pathology change were studied under microscope about nasal and bronchi mucosa and bronchoalveolar lavege fluid cells. IL-4 mRNA and IFN-gamma mRNA in lung tissue were detected through RT-PCR, the protein production of IL-4 and IFN-gamma about bronchoalveolar lavege fluid and serum were detected through ELISA.

Results: In ovalbumin-sensitized group, allergic animal model were made successfully. In only BCG used transairway group, the symptoms of animals were normal, few inflammation cells infiltrated into the mucosa of nasal and bronchi, the numbers of macrophage were greatly increased in smear of bronchoalveolar lavege fluid, IFN-gamma mRNA and protein production were greatly increased. In BCG used transairway + ovalbumin-sensitized group, the allergic symptoms and inflammation were greatly reduced, not only IFN-gamma mRNA and protein production were increased but also IL-4 mRNA and protein production were greatly decreased.

Conclusion: It is a good pathway that BCG used transairway. The immunoloregulation mechanisms of BCG to allergic airway diseases are to enhance Th1 response, in the meantime, to suppress Th2 response.
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August 2002

[Hypoxia effects on vascular endothelial growth factor derived epithelial cells of nasal polyps].

Zhonghua Er Bi Yan Hou Ke Za Zhi 2002 Feb;37(1):34-7

Department of Otorhinolaryngology, Head and Neck Surgery, China-Japan Union Hospital of Jilin University, Changchun 130031, China.

Objective: To understand the role of nasal mucous epithelial cells to hypoxia in early stage of nasal polyps(NP) formation.

Methods: Epithelial cells of NP and inferior turbinate (IT) were cultured without serum under normal oxygen and hypoxia, and stimulus of inflammatory cytokines. Erythropoietin (EPO) was regarded as hypoxia mark, and expression of vascular endothelial growth factor(VEGF) mRNA and protein derived from epithelial cells were detected respectively by in situ hybridization and ELISA.

Results: 1. Under hypoxia, EPO mRNA was expressed intensely in epithelial cells from NP and IT, and there was no significant difference between both of them. This result suggested that EPO might be regarded as a hypoxic mark. 2. The ability of producing VEGF mRNA increased with cytokines stimulation, especially under hypoxia. Protein level of VEGF from epithelial cells of NP and IT increased with cytokines stimulation, especially in hypoxia and was time-dependent.

Conclusion: Epithelial cells actively produce vast VEGF under hypoxia. The VEGF induced by hypoxia of the mucosa in middle meatus is of importance in the formation of nasal polyps(NP) in early stage, which may be the major cause of NP formation in middle meatus.
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February 2002

[Analysis of sever and rare complications of transsphenoidal surgery for lesions of sella turcica].

Zhonghua Er Bi Yan Hou Ke Za Zhi 2002 Apr;37(2):124-6

Department of Otorhinolaryngology, China-Japan Union Hospital, Jilin University, Changchun 130031, China.

Objective: To analysis the reason and prevention of sever and rare complications of transsphenoidal surgery for lesions of sella turcica.

Methods: The clinical data of 16 cases who occurred sever and rare complications during transsphenoidal surgery for 200 cases of lesions of sella turcica was analyzed. The reason and prophylaxis of complications was inquired.

Results: The sever and rare complications occurred in 16 cases. 4 cases died and 8 cases bleed severly and the version of 2 cases was deteriorated and 2 cases had mental disorder and 2 cases had epilepticism and 1 cases had sever postoperative headache and the brain was damaged in 2 cases among 16 cases.

Conclusion: Although the transsphenoidal surgery comparing with transcranial surgery has mini-invasive, many complications can occur and can be decreased by selecting surgical accommodation strictly and similarity with anatomy of sella turcica and mastering transsphenoidal surgical technique skilled.
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April 2002

[Molecular biological study of aloe vera in the treatment of experimental allergic rhinitis in rat].

Lin Chuang Er Bi Yan Hou Ke Za Zhi 2002 May;16(5):229-31

Department of Otolaryngology-Head and Neck Surgery, China-Japan Union Hospital, Jilin University, Changchun 130031.

Objective: To study the therapeutic mechanism of aloe vera in allergic rhinitis (AR).

Method: Ovalbumin sensitized white rat used as animal models of AR were treated intranasally with aloe vera. At the end of treatment, the differences in the behavior science were observed; the changes in the nasal mucosa were studied by pathological; IL-2, IL-4 mRNA in the nasal mucosa and spleen were used to do reverse transcriptive polymerase chain reaction (RT-PCR).

Result: The behavior science score of positive controls (8.42 +/- 1.06) was higher than the experimental group (2.02 +/- 0.42) and normal controls (0); inflammatory reactions in the experimental group nasal mucosa were remarkably relieved; the mean expression level of IL-2 mRNA in the experimental group was higher significantly than positive controls (P < 0.01); but that of IL-4 mRNA was lower evidently (P < 0.01).

Conclusion: The aloe vera are suggested to be involved in the differentiation of CD4+ lymphocytes, by means of regulating the expression of Th1 and Th2 cytokines. The results suggests that local aloe vera treatment was a selective and non-traumatic method to treat the allergic rhinitis.
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May 2002

[The nasosinus inverted papilloma with intracranial extension].

Lin Chuang Er Bi Yan Hou Ke Za Zhi 2002 May;16(5):196-7

Department of Otolaryngology-Head and Neck Surgery, China-Japan Union Hospital, Jilin University, Changchun 130031.

Objective: To study the treatment of inverted papilloma (NIP) with intracranial extension.

Method: Nine patients of NIP all suffered from the operation. Six patients with anterior skull base erosion suffered from large tumor resections via combined craniofacial and cranial approach. Three patients suffered from small tumor resections via transfrontal and transethmoidal sinus approach. The defects of basicranial bone and dura were repaired at the same time. Five patients were found the tissue canceration in the postoperation.

Result: In 7 patients followed-up from 3 to 13 years, no one has recurrenced.

Conclusion: The canceration probability of NIP with intracranial extension should be sufficiently considered. The tumor resection via combined craniofacial approach or transfrontal approach must be based on the tumor size and extension, and removed completely.
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May 2002

Expression and significance of the vascular permeability factor in nasal polyps.

Chin Med J (Engl) 2002 Aug;115(8):1251-2

Department of ENT, Second Affiliated Hospital, Wenzhou Medical College, Wenzhou, China.

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August 2002

[Expression of IL-12 mRNA in the nasal mucosa of experimental allergic rhinitis].

Lin Chuang Er Bi Yan Hou Ke Za Zhi 2002 Aug;16(8):423-5

Department of Otorhinolaryngology-Head and Neck Surgery, China-Japan Union Hospital of Jilin University, Changchun 130031.

Objective: To study the relation between the level of IL-12 expression and the pathogenesis of allergic rhinitis, we investigated the expression of interleukin-12 (IL-12) mRNA in the nasal mucosa of experimental allergic rhinitis.

Method: 24 healthy guinea pigs were randomly divided into two groups. A model of allergic rhinitis (AR) in guinea pig was established by using ovalbumin intraperitoneal immunization and nasal antigen challenge. The nasal mucosa obtained from AR models as well as normal controls were studied for histopathological changes with stain HE, and expression of IL-12 mRNA with RT-PCR.

Result: IL-12 was expressed in all the samples from AR models to normal controls. The mean expression level of IL-12 gene, however, in AR models was lower than that in normal controls (0.667 +/- 0.104 vs 0.847 +/- 0.071, P < 0.01).

Conclusion: IL-12 expression was decreased in the nasal mucosa of allergic rhinitis, which plays an important role in the pathogenesis of allergic rhinitis. It is also suggested that IL-12 replacement therapy may be a new approach to the treatment of allergic rhinitis.
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August 2002
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