Publications by authors named "Zhangyan Shi"

8 Publications

  • Page 1 of 1

Study on effects of sweating treatment and different drying methods on the quality of Gentianae macrophyllae Radix.

Sci Rep 2021 May 6;11(1):9717. Epub 2021 May 6.

College of Life Science, Northwest University, North Tabai Road, Beilin District, Xi'an, 710069, People's Republic of China.

To provide basis for improving the quality of Gentianae macrophyllae Radix and optimising the field processing method. Gentianae macrophyllae Radix samples were collected from Long County. Five main active iridoids were determined by using HPLC. The HPLC fingerprints were measured and analysed by the traditional Chinese medicine chromatographic fingerprint similarity evaluation system. Water, total ash, acid insoluble ash and ethanol extract contents were determined in accordance with Chinese Pharmacopoeia (2015). Colour of the surface and powdered samples were determined by using a colorimeter. Data were analysed by SPSS11.0. Notable differences were found among samples from different sweating and drying processes, in spite of the relatively consistent overall chemical characteristics. Sweating and drying methods influenced the contents of some active ingredients and colour very significantly; moreover, they also showed significant effects on the water, ash, and ethanol extract contents. The necessity of sweating treatment in the field processing of Gentianae macrophyllae Radix may be insufficient. Drying in the shade and oven drying are more profitable for preserving the active constituents. Gentianae macrophyllae Radix should be dried directly without sweating, and drying in the shade and oven drying should be adopted preferentially to improve the quality.
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http://dx.doi.org/10.1038/s41598-021-88511-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8102551PMC
May 2021

Genetic Variants of the 9 Gene Are Associated with Nonspecific Intellectual Disability: A Family-Based Association Study.

Genet Test Mol Biomarkers 2020 Oct 29;24(10):625-631. Epub 2020 Sep 29.

School of Medicine, Northwest University, Xi'an, China.

Mutations within the myotubularin-related protein 9 gene (9) have been identified in several families with nonsyndromic intellectual disability (NSID), a generalized neurodevelopmental disorder; however, the relationship between 9 and NSID needs to be verified using a larger sample size. To explore whether genetic variants in the 9 gene are linked to susceptibility of NSID among the Chinese population. Seven single nucleotide polymorphisms (SNPs) of the 9 gene (rs4559208, rs3824211, rs2164272, rs2164273, rs1897951, rs6991606, and rs7815802) were analyzed using family-based association testing among 258 Han Chinese NSID families. Three SNPs of 9 were significantly associated with NSID ( = 2.152,  = 0.031 for rs4559208;  = 2.403,  = 0.016 for rs2164273; and  = 2.758,  = 0.006 for rs7815802). Three alleles of these SNPs were more likely to be transferred from the carrier parents to the affected offspring. Haplotypes constructed using these SNPs also showed a similar transmitting trend ( = 2.505,  = 0.012,  = 8.835, and global  = 0.032). Carriers with the G-G-C haplotype showed a higher risk of NSID (odds ratio = 1.46, 95% confidence interval [1.01-2.09],  = 0.04) than others. functional predictions supported an etiological role for these three SNPs in NSID biology. This study provides additional insights into the association of NSID with specific alleles, and haplotypes within the 9 gene. Genotypic analyses of the 9 gene should be considered for patients presenting with NSID of unknown etiology.
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http://dx.doi.org/10.1089/gtmb.2020.0145DOI Listing
October 2020

Mutations of ARX and non-syndromic intellectual disability in Chinese population.

Genes Genomics 2019 01 25;41(1):125-131. Epub 2018 Sep 25.

Key Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education), Institute of Population and Health, Northwest University, Xi'an, 710069, China.

Mutations of Aristaless-related homeobox (ARX) gene were looked as the third cause of non-syndromic intellectual disability (NSID), while the boundary between true disease-causing mutations and non-disease-causing variants within this gene remains elusive. To investigate the relationship between ARX mutations and NSID, a panel comprising six reported causal mutations of the ARX was detected in 369 sporadic NSID patients and 550 random participants in Chinese. Two mutations, c.428_451 dup and p.G286S, may be disease-causing mutations for NSID, while p.Q163R and p.P353L showed a great predictive value in female NSID diagnosis with significant associations (X = 19.60, p = 9.54e-6 for p.Q163R; X = 25.70, p = 4.00e-07 for p.P353L), carriers of these mutations had an increased risk of NSID of more than fourfold. Detection of this panel also predicted significant associations between genetic variants of the ARX gene and NSID (p = 3.73e-4). The present study emphasized the higher genetic burden of the ARX gene on NSID in the Chinese population, molecular analysis of this gene should be considered for patients presenting NSID of unknown etiology.
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http://dx.doi.org/10.1007/s13258-018-0745-6DOI Listing
January 2019

Genetic Polymorphism of GABRR2 Modulates Individuals' General Cognitive Ability in Healthy Chinese Han People.

Cell Mol Neurobiol 2017 Jan 27;37(1):93-100. Epub 2016 Feb 27.

Key Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education), Institute of Population and Health, College of Life Science, Northwest University, Xi'an, 710069, China.

Previous studies have indicated that the cognitive impairment or deficit is associated with GABAergic signaling in central nervous system. Inspired by the finding that receptor GABRR2 modulates concentration of GABA and phasic inhibitory GABAergic transmission in brain. This study investigated to what extent a genetic variant (c.1423C>T, rs282129) of GABRR2 gene modulates individuals' general cognitive ability in 987 Chinese Han people. Results showed a significant influence of GABRR2 gene polymorphism on individuals' Raven's Standard Progressive Matrices (RSPM) performance (F = 3.58, P = .028 by ANOVA and χ  = 9.35, P = .009 by K-W test, respectively), even if non-genetic factors were partialed out (gender, major, types of birthplace, and socioeconomic index) (B = -.67, SE = .26, t = 2.63, P = .009). The finding provided a strong evidence, to our knowledge, for the view that genetic variant of GABRR2 gene may contribute to the difference of individuals' general cognitive ability, independently.
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http://dx.doi.org/10.1007/s10571-016-0347-2DOI Listing
January 2017

Polymorphic variation in CHAT gene modulates general cognitive ability: An association study with random student cohort.

Neurosci Lett 2016 Mar 6;617:122-6. Epub 2016 Feb 6.

Key Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education), Institute of Population and Health, College of Life Science, Northwest University, Xi'an 710069, China. Electronic address:

The choline O-acetyltransferase (CHAT) gene has been associated with various human disorders that involve cognitive impairment or deficiency. However, the influence of disease-associated variants of CHAT on normal individuals remains dubious. Here we demonstrated the impact of CHAT sequence variants (G-120A) on general human cognitive ability in a cohort of 750 Chinese undergraduate students. A multiple choice questionnaire was used to obtain basic demographic information, such as parents' occupations and education levels. We also administered and scored the Raven's Standard Progressive Matrices (RSPM). A one-way analysis of variance (ANOVA) and Kruskal-Wallis test (K-W) revealed a significant association between sequence polymorphisms of G-120A and individuals' Raven score (p=0.031 for ANOVA and p=0.026 for K-W tests). Moreover, further hierarchical analysis showed a similar trend in the association between G-120A variants and Raven scores only in the female subjects (p=0.008 for ANOVA and p=0.024 for K-W tests) but not in the male subjects. The results of a multiple linear regression confirmed that after we controlled gender, age, birthplace and other non-genetic factors, CHAT G-120A polymorphisms still significantly influenced individual Raven scores (B=-0.70, SE=0.28, t=-2.50, p=0.013). Our results demonstrated that sequence variants of CHAT were associated with human cognitive ability in not only patients with psychiatric disorders but also normal healthy individuals. However, some issues remained indeterminable, such as gender differences and the extent of the influence on individuals' general cognitive abilities; thus, the further research using an independent random sample was required.
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http://dx.doi.org/10.1016/j.neulet.2016.02.002DOI Listing
March 2016

A New Role for LOC101928437 in Non-Syndromic Intellectual Disability: Findings from a Family-Based Association Test.

PLoS One 2015 19;10(8):e0135669. Epub 2015 Aug 19.

Key Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education), College of Life Science, Institute of Population and Health, Northwest University, Xi'an, China.

Non-syndromic intellectual disability (NSID) is mental retardation in persons of normal physical appearance who have no recognisable features apart from obvious deficits in intellectual functioning and adaptive ability; however, its genetic etiology of most patients has remained unknown. The main purpose of this study was to fine map and identify specific causal gene(s) by genotyping a NSID family cohort using a panel of markers encompassing a target region reported in a previous work. A total of 139 families including probands, parents and relatives were included in the household survey, clinical examinations and intelligence tests, recruited from the Qinba mountain region of Shannxi province, western China. A collection of 34 tagged single nucleotide polymorphisms (tSNPs) spanning five microsatellite marker (STR) loci were genotyped using an iPLEX Gold assay. The association between tSNPs and patients was analyzed by family-based association testing (FBAT) and haplotype analysis (HBAT). Four markers (rs5974392, rs12164331, rs5929554 and rs3116911) in a block that showed strong linkage disequilibrium within the first three introns of the LOC101928437 locus were found to be significantly associated with NSID (all P<0.01) by the FBAT method for a single marker in additive, dominant and recessive models. The results of haplotype tests of this block also revealed a significant association with NSID (all P<0.05) using 2-window and larger HBAT analyses. These results suggest that LOC101928437 is a novel candidate gene for NSID in Han Chinese individuals of the Qinba region of China. Although the biological function of the gene has not been well studied, knowledge about this gene will provide insights that will increase our understanding of NSID development.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0135669PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4545728PMC
May 2016

[Comparison research on quality of cultivated and wild Anemarrhena Rhizome from Yi County].

Zhongguo Zhong Yao Za Zhi 2011 Sep;36(17):2316-20

Resource Biology and Modern Biotechnology Key Laboratory in Western China of Education Ministry College of Life Science Northwest University, Xi'an 710069, China.

Objective: To compare the quality of cultivated and wild Anemarrhena Rhizome from Yi County (Xiling Zhimu) based on contents analysis of active constituents.

Method: Samples of cultivated Anemarrhena Rhizome from most townships of Yi County were analyzed and compared with wild ones. Six indexes belonged to three kinds active constituents of saponin, flavornoid and polysaccharide were adopted. HPLC-ELSD method with cholesterol as internal standard was adopted to determine the content of sarsasapongenin. HPLC-ELSD method was used to simultaneously determine the contents of anemasaponin C and Anemasaponin A III. Contents of neomangiferin and mangiferin were determined by HPLC-UV method. Total polysaccharide was determined by phenol sulfate method.

Result: The mean content of sarsasapongenin in cultivated Anemarrhena Rhizome samples is slightly lower than the wild. The mean contents of anemasaponin C and Anemasaponin A III in cultivated Anemarrhena Rhizome samples are higher than the wild. There is no notable difference of these three index between the cultivated and the wild. The cultivated Anemarrhena Rhizome samples have a lower content of neomangiferin and a higher content of mangiferin than the wild. While the total content of these two flavonoids have no notable difference. The cultivated Anemarrhena Rhizome samples have a higher content of total polysaccharide than the wild samples.

Conclusion: Contents of active constituents in cultivated Anemarrhena Rhizome from Yi County (Xiling Zhimu) are not notably different with the wild Anemarrhena Rhizome. They have similar good quality as the wild ones.
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September 2011

Polymorphisms in the DLG3 gene is not associated with non-syndromic mental retardation in the Chinese Han population of Qin-Ba mountain.

Cell Mol Neurobiol 2011 Jul 3;31(5):695-700. Epub 2011 Mar 3.

Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Institute of Population and Health, College of Life Science, Northwest University, People's Republic of China.

Discs-large-related 3 (DLG3), a member of the membrane-associated guanylate kinases (MAGUKs) protein family, playing an important role in regulating NMDA signal pathway and contributing to synaptic plasticity, may have an influence on the susceptibility of non-syndromic mental retardation (NSMR). To investigate the possible genetic contribution of DLG3 gene to the NSMR of Chinese Han population, we performed an association study of 556 subjects (118 NSMR, 116 borderline NSMR, and 322 controls in 275 males and 281 females) from Qin-Ba mountain region of Shaanxi province in the northwest of China by five common SNPs in the gene. The results showed that there was no positive association between the genetic variations of DLG3 and NSMR. In conclusion, the results of this study indicated that DLG3 did not associate with NSMR in Chinese Han population; however, further studies are needed.
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http://dx.doi.org/10.1007/s10571-011-9666-5DOI Listing
July 2011