Publications by authors named "Zhang Yi"

8,411 Publications

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Long noncoding RNA IL6-AS1 is highly expressed in chronic obstructive pulmonary disease and is associated with interleukin 6 by targeting miR-149-5p and early B-cell factor 1.

Clin Transl Med 2021 Jul;11(7):e479

National Center for Respiratory Medicine, State Key Laboratory of Respiratory Disease & National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, 151 Yanjiang Xi Road, Guangzhou, Guangdong, 510000, China.

Chronic obstructive pulmonary disease is a complex condition with multiple etiologies, including inflammation. We identified a novel long noncoding RNA (lncRNA), interleukin 6 antisense RNA 1 (IL6-AS1), which is upregulated in this disease and is associated with airway inflammation. We found that IL6-AS1 promotes the expression of inflammatory factors, especially interleukin (IL) 6. Mechanistically, cytoplasmic IL6-AS1 acts as an endogenous sponge by competitively binding to the microRNA miR-149-5p to stabilize IL-6 mRNA. Nuclear IL6-AS1 promotes IL-6 transcription by recruiting early B-cell factor 1 to the IL-6 promoter, which increases the methylation of the H3K4 histone and acetylation of the H3K27 histone. We propose a model of lncRNA expression in both the nucleus and cytoplasm that exerts similar effects through differing mechanisms, and IL6-AS1 probably increases inflammation via multiple pathways.
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http://dx.doi.org/10.1002/ctm2.479DOI Listing
July 2021

Sphingosine 1-phosphate Stimulates Insulin Secretion and Improves Cell Survival by Blocking Voltage-dependent K Channels in β Cells.

Front Pharmacol 2021 12;12:683674. Epub 2021 Jul 12.

Department of Pharmacology, Shanxi Medical University, Taiyuan, China.

Recent studies suggest that Sphingosine 1-phosphate (S1P) plays an important role in regulating glucose metabolism in type 2 diabetes. However, its effects and mechanisms of promoting insulin secretion remain largely unknown. Here, we found that S1P treatment decreased blood glucose level and increased insulin secretion in C57BL/6 mice. Our results further showed that S1P promoted insulin secretion in a glucose-dependent manner. This stimulatory effect of S1P appeared to be irrelevant to cyclic adenosine monophosphate signaling. Voltage-clamp recordings showed that S1P did not influence voltage-dependent Ca channels, but significantly blocked voltage-dependent potassium (Kv) channels, which could be reversed by inhibition of phospholipase C (PLC) and protein kinase C (PKC). Calcium imaging revealed that S1P increased intracellular Ca levels, mainly by promoting Ca influx, rather than mobilizing intracellular Ca stores. In addition, inhibition of PLC and PKC suppressed S1P-induced insulin secretion. Collectively, these results suggest that the effects of S1P on glucose-stimulated insulin secretion (GSIS) depend on the inhibition of Kv channels the PLC/PKC signaling pathway in pancreatic β cells. Further, S1P improved β cell survival; this effect was also associated with Kv channel inhibition. This work thus provides new insights into the mechanisms whereby S1P regulates β cell function in diabetes.
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http://dx.doi.org/10.3389/fphar.2021.683674DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8313013PMC
July 2021

Targeting neoantigens for cancer immunotherapy.

Biomark Res 2021 Jul 28;9(1):61. Epub 2021 Jul 28.

Biotherapy Center & Cancer Center, The First Affiliated Hospital of Zhengzhou University, 450052, Zhengzhou, China.

Neoantigens, a type of tumor-specific antigens derived from non-synonymous mutations, have recently been characterized as attractive targets for cancer immunotherapy. Owing to the development of next-generation sequencing and utilization of machine-learning algorithms, it has become feasible to computationally predict neoantigens by depicting genetic alterations, aberrant post-transcriptional mRNA processing and abnormal mRNA translation events within tumor tissues. Consequently, neoantigen-based therapies such as cancer vaccines have been widely tested in clinical trials and have demonstrated promising safety and efficacy, opening a new era for cancer immunotherapy. We systematically summarize recent advances in the identification of both personalized and public neoantigens, neoantigen formulations and neoantigen-based clinical trials in this review. Moreover, we discuss future techniques and strategies for neoantigen-based cancer treatment either as a monotherapy or as a combination therapy with radiotherapy, chemotherapy or immune checkpoint inhibitors.
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http://dx.doi.org/10.1186/s40364-021-00315-7DOI Listing
July 2021

Machine Learning-Enabled High-Resolution Dynamic Deuterium MR Spectroscopic Imaging.

IEEE Trans Med Imaging 2021 Jul 28;PP. Epub 2021 Jul 28.

Deuterium magnetic resonance spectroscopic imaging (DMRSI) has recently been recognized as a potentially powerful tool for noninvasive imaging of brain energy metabolism and tumor. However, the low sensitivity of DMRSI has significantly limited its utility for both research and clinical applications. This work presents a novel machine learning-based method to address this limitation. The proposed method synergistically integrates physics-based subspace modeling and data-driven deep learning for effective denoising, making high-resolution dynamic DMRSI possible. Specifically, a novel subspace model was used to represent the dynamic DMRSI signals; deep neural networks were trained to capture the low-dimensional manifolds of the spectral and temporal distributions of practical dynamic DMRSI data. The learned subspace and manifold structures were integrated via a regularization formulation to remove measurement noise. Theoretical analysis, computer simulations, and in vivo experiments have been conducted to demonstrate the denoising efficacy of the proposed method which enabled high-resolution imaging capability. The translational potential was demonstrated in tumor-bearing rats, where the Warburg effect associated with cancer metabolism and tumor heterogeneity were successfully captured. The new method may not only provide an effective tool to enhance the sensitivity of DMRSI for basic research and clinical applications but also provide a framework for denoising other spatiospectral data.
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http://dx.doi.org/10.1109/TMI.2021.3101149DOI Listing
July 2021

Alkoxy cyanoacrylate-based nanoparticles with stealth and brain-targeting properties.

J Drug Target 2021 Jul 28:1-33. Epub 2021 Jul 28.

State Key Laboratory of Toxicology and Medical Countermeasures, Beijing Institute of Pharmacology and Toxicology, 27 Taiping road, Beijing, 100850, China.

Nanoparticles (NPs) with "stealth" properties have been designed to decrease the phagocytosis of such particles by mononuclear phagocytes and to protect them from enzymatic degradation, thus improving circulation time and bioavailability after intravenous administration. Brain-targeting modifications endow NPs with the capacity to cross the blood-brain barrier, facilitating chemotherapy for brain diseases such as glioma. In this study, newly designed alkoxy cyanoacrylate-based NPs with stealth and brain-targeting properties were synthesized and evaluated. The monomers for NP core polymerization were chemically modified to hydrophilic short alkoxy structure for stealth purposes and coated with polysorbate-80 for brain targeting. Two monomers [2-methoxyethyl cyanoacrylate and 2-(2-methoxyethyl)ethyl cyanoacrylate] were used to create NP and NP, respectively. Both NPs were successfully loaded with antisense oligonucleotide (ASON) of transforming growth factor beta 2. Compared to traditional n-butyl cyanoacrylate-based ASON-NP, ASON-NP was found to decrease phagocytosis by mononuclear macrophages (RAW264.7) and to increase cellular uptake by cancer cells. ASON-NP showed definite brain targeting and anti-cancer effects. This work provides a potential new strategy for preparing stealth NPs core, providing a new NP vehicle for clinical drug delivery that may be targeted to the brain and circulates in the blood for an extended period of time.
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http://dx.doi.org/10.1080/1061186X.2021.1961790DOI Listing
July 2021

10-Day and 14-day high-dose dual therapy for the treatment of Helicobacter pylori: A propensity score matching analysis.

Helicobacter 2021 Jul 28:e12833. Epub 2021 Jul 28.

Department of Gastroenterology, Daping Hospital, Army Medical University, Chongqing, China.

Background: Inconsistent eradication rates for Helicobacter pylori have been reported worldwide with dual therapy, perhaps owing to the difference in dose administration and treatment duration. This retrospective study aimed to determine whether high-dose dual therapy (HDDT) with different regimens leads to different eradication rates. The study compares the efficacy and safety of HDDT 10-day vs 14-day and investigates the factors that might affect the eradication rates.

Materials And Methods: Two comparable treatment groups were based on propensity score matching (PSM). Patients were divided into two groups based on the therapy they underwent: 10-day HDDT and 14-day HDDT (20 mg esomeprazole and 750 mg amoxicillin, administered four times daily). The eradication rates, adverse events (AEs), patient compliance, CYP2C19 gene polymorphisms, and antibiotic resistance rates of the two groups were compared.

Results: The intention to treat (ITT) analysis showed that the eradication rates for 10-day and 14-day groups were 78.4% (95% CI 69.6%-87.2%) and 89.7% (95% CI 83.3%-96.2%; p = .039), respectively, while the per-protocol (PP) eradication rates were 80.0% (95% CI 71.3%-88.7%) and 92.9% (95% CI 87.4%-98.5%; p = .014), respectively. The corresponding drug-related AEs were 6.8% (6/88) and 5.7% (5/88; p = .755). No significant differences were observed between the compliance rates of the two groups. The CYP2C19 gene polymorphism had no effect on the eradication rates of the two groups.

Conclusion: The results showed that the 14-day HDDT affords a higher H. pylori eradication rate than the 10-day HDDT.
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http://dx.doi.org/10.1111/hel.12833DOI Listing
July 2021

DiLFM: an artifact-suppressed and noise-robust light-field microscopy through dictionary learning.

Light Sci Appl 2021 Jul 27;10(1):152. Epub 2021 Jul 27.

Department of Automation, Tsinghua University, Beijing, 100084, China.

Light field microscopy (LFM) has been widely used for recording 3D biological dynamics at camera frame rate. However, LFM suffers from artifact contaminations due to the illness of the reconstruction problem via naïve Richardson-Lucy (RL) deconvolution. Moreover, the performance of LFM significantly dropped in low-light conditions due to the absence of sample priors. In this paper, we thoroughly analyze different kinds of artifacts and present a new LFM technique termed dictionary LFM (DiLFM) that substantially suppresses various kinds of reconstruction artifacts and improves the noise robustness with an over-complete dictionary. We demonstrate artifact-suppressed reconstructions in scattering samples such as Drosophila embryos and brains. Furthermore, we show our DiLFM can achieve robust blood cell counting in noisy conditions by imaging blood cell dynamic at 100 Hz and unveil more neurons in whole-brain calcium recording of zebrafish with low illumination power in vivo.
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http://dx.doi.org/10.1038/s41377-021-00587-6DOI Listing
July 2021

RNA modifications act as regulators of cell death.

RNA Biol 2021 Jul 27:1-11. Epub 2021 Jul 27.

Department of General Surgery, Affiliated Hospital of Xuzhou Medical University, Xuzhou, China.

Currently, more than one hundred types of RNA modifications have been found, and many of these modifications are reversible and dynamically regulated. RNA modifications can regulate RNA stability and translation and are thus involved in multiple biological activities. Recently, RNA modifications have been shown to have important roles in the regulation of cell death. Cell death is a critical process that maintains tissue homoeostasis and is regulated by multiple pathways in response to specific stimuli. In this review, we summarize the current understanding of the roles of RNA modifications in cell death mediation and discuss the prospects of such research. mA, N-Methyladenosine; mAm, N,2'-O-Dimethyladenosine; mA, N-Methyladenosine; mC, 5-Methylcytosine; hmC, 5-Hydroxymethylcytosine; Ψ, pseudouridine; A-to-I, adenosine-to- inosine; hnRNPs, heterogeneous nuclear ribonucleoproteins; MOMP, mitochondrial outer membrane permeabilization; DD, death domain; DISC, death-inducing signalling complex; DED, death effector domain; FADD, FAS-associated protein with the death domain; TRADD, TNF receptor-associated protein with death domain; CMA, chaperone- mediated autophagy; PE, phosphatidylethanolamine; AD, alzheimer's disease; AML, acute myeloid leukaemia; miR, microRNA; 6-OHDA, 6-hydroxydopamine hydrochloride; R-2HG, R-2-hydroxyglutarate; IRES, internal ribosome entry site; BMSCs, bone-derived mesenchymal stem cells; NPCs, nucleus pulposus cells; HsCG, human chorionic gonadotropin; snoRNAs, small nucleolar RNAs; ER, endoplasmic reticulum; lncRNAs, long noncoding RNAs; TNM, tumour-node-metastasis.
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http://dx.doi.org/10.1080/15476286.2021.1925460DOI Listing
July 2021

[Effects of brown rice on metabolomics related to glucose and lipid in normal rats].

Wei Sheng Yan Jiu 2021 Jul;50(4):600-608

National Health and Family Planning Commission, National Institute for Nutrition and Health, China CDC, Beijing 100050, China.

Objective: To investigate the effects of whole grain rice on blood sugar, blood lipid and metabonomics compared with ordinary feed and refined rice.

Methods: According to the initial body weight and fasting blood glucose, 36 male SD rats were randomly divided into 3 groups: control group, RG-rice group and WG-rice group, fed with AIN-93 G feed, feeds prepared with refined rice flour or brown rice flour, respectively, for 18 weeks. Food intake and body weight were monitored weekly. At the end of the experiment, abdominal aortic blood was taken to measure blood glucose and blood lipids, and untargeted metabolomics was performed.

Results: During the experiment, the body weight growth and food intake of rats in each group were basically the same. Fasting blood glucose(FBG) in RG-rice group were(10.12±1.51)mmol/L, which were significantly higher than those in other groups, hepatic glycogen in RG-rice group were(0.90±0.33)mg/g, which were significantly higher than those in WG-rice group. TC and LDL in RG-rice group were(1.85±0.21)mmol/L and(0.84±0.10)mmol/L, respectively, which were significantly lower than those in control group and RG-rice group(P& lt; 0.05). The non-targeted metabolomics result were compared in pairs. A total of 30 metabolic differences were found between the control and RG-rice groups, involving 8 potential pathways, 12 metabolic differences were found between the control and WG-rice groups, involving 2 potential pathways, 18 metabolic differences were found between the RG-rice and WG-rice groups, involving 4 potential pathways. Pathway enrichment was performed again according to the correlation analysis of differential metabolites with blood glucose and blood lipids. The result showed that the FBG-related pathways include glycerophospholipid metabolism and glutathione metabolism. TG-related pathways include glycine, serine and threonine metabolism, ether lipid metabolism, glycerophospholipid metabolism, and glyoxylic acid and dicarboxylic acid metabolism. TC-related pathways include D-glutamine and D-glutamate metabolism, glycine, serine and threonine metabolism, alanine, aspartic acid and glutamate metabolism, arginine biosynthesis, glycerophospholipid metabolism, glutathione metabolism, and glyoxylic acid and dicarboxylic acid metabolism. LDL-related pathway include glycerophospholipid metabolism.

Conclusion: Eating more refined rice may increase blood sugar, brown rice helps to maintain a relatively low blood lipid level. Glycerol phospholipid metabolism is a common pathway that rice affects blood glucose and blood lipid metabolism in normal rats.
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http://dx.doi.org/10.19813/j.cnki.weishengyanjiu.2021.04.011DOI Listing
July 2021

Gelation of a Pentapeptide in Alcohols.

Langmuir 2021 Jul 23. Epub 2021 Jul 23.

Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.

Properties of solvents such as polarity and H-bond-forming ability are critical for the formation of an organogel and have a significant impact on the gel behavior, as solvents are the majority of organogel systems. However, so far, there is still a lack of systematic studies regarding the effects of molecular structures of solvents on the characteristics of organogels. Motivated by revealing such a relationship, in this paper, we studied the morphologies of assemblies, gelation behaviors, and secondary structures of a pentapeptide termed EAF-5 in a wide variety of alcohols. The side chains and lengths of carbon chains of the solvent molecules were found to play a critical role in the self-assembly and gelation of EAF-5. EAF-5 was capable of self-assembling into fibers and entangling into a network in alcohols including ethanol, propanol, butanol, -pentanol, and -hexanol, which further immobilized the corresponding alcohols to form gels. In these organogels, increasing β-sheet secondary structures of the peptides were formed by introducing side chains and extending the length of primary alcohol molecules. We hypothesized that alcohol molecules with extended lengths and side chains reduced the gelator-solvent interactions and promoted the gelator-gelator interactions, resulting in the self-assembly of EAF-5 into fibril structures and development of gels. These findings provide a new sight into the interactions between gelators and solvents and are helpful for designing peptide-based organogelators.
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http://dx.doi.org/10.1021/acs.langmuir.1c00841DOI Listing
July 2021

Obtaining of Mesoporous Aluminosilicates with High Hydrothermal Stability by Composite Organic Templates: Utility and Mechanism.

Langmuir 2021 Jul 23. Epub 2021 Jul 23.

Petrochemical Research Institute, Petrochina Company Limited, Beijing 100195, P. R. China.

We have reported the synthesis of mesoporous aluminosilicates (MAs) with high hydrothermal stability via assembly of basic characteristic structure units of typical microporous zeolite Y. In spite of this, high consumption of organic template and HO remains a major obstacle to its industrial application. Herein, a facile and effective strategy called "composite templates" was employed to decrease significantly the amount of P123 and HO. In this method, composite micelles of P123/poly(vinyl alcohol) (PVA) could be more easily dispersed in the solution due to the lowering of water's surface tension caused by the free hydroxyl groups of PVA. Moreover, the improved assembly ability of composite micelles in high concentration solution leads to the synthesis of hydrothermally stable MAs with 45% decrease of organic template P123 and 81% that of water amount. It was found that by the introduction of composite templates, the textural properties such as the surface area of materials, volume of pore, size of pore, and thickness were enlarged simultaneously. Meanwhile, this article presented an understanding into the assembly of composite micelles in the process of synthesis of MAs.
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http://dx.doi.org/10.1021/acs.langmuir.1c01151DOI Listing
July 2021

Synthesis of CF-Containing Linear Nitriles from α-(Trifluoromethyl)styrenes.

Org Lett 2021 Jul 23. Epub 2021 Jul 23.

Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology (ECUST), Shanghai 200237, China.

Four unprecedented base-catalyzed/mediated nucleophilic additions of TMSCN to α-(trifluoromethyl)styrenes and 2-trifluoromethyl enynes were developed. The reaction proceeded smoothly at room temperature under mild and transition-metal-free conditions without affecting the trifluoromethyl group and afforded the corresponding CF-containing alkyl, alkynyl, and butadienyl nitriles in moderate to excellent yields in a highly regioselective manner, respectively.
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http://dx.doi.org/10.1021/acs.orglett.1c01988DOI Listing
July 2021

Chiral nanomaterials for tumor therapy: autophagy, apoptosis, and photothermal ablation.

J Nanobiotechnology 2021 Jul 22;19(1):220. Epub 2021 Jul 22.

Department of Breast Surgery, Southwest Hospital, Army Medical University, Chongqing, 400038, China.

Chirality is a fundamental characteristic of natural molecules and a crucial factor in the biochemical reactions of living cells and organisms. Recently, researchers have successfully introduced chiral molecules to the surfaces of nanomaterials, creating chiral nanomaterials that exhibit an upscaling of chiral behavior from the molecular scale to the nanoscale. These chiral nanomaterials can selectively induce autophagy, apoptosis, and photothermal ablation in tumor cells based on their chirality, making them promising for application in anti-tumor therapy. However, these interesting and important phenomena have hitherto received little attention. Accordingly, we herein present a review of recent research progress in the field of chiral nanomaterials for tumor therapy along with brief looks at the mechanistic details of their actions. Finally, the current challenges and future perspectives of chiral nanomaterials in terms of maximizing their potential in tumor therapy are discussed. Thus, this review provides a helpful introduction to the design of chiral nanomaterials and will hopefully highlight the importance of chirality in tumor therapy.
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http://dx.doi.org/10.1186/s12951-021-00965-7DOI Listing
July 2021

Protective role of microRNA-31 in acetaminophen-induced liver injury: a negative regulator of c-Jun N-terminal kinase (JNK) signaling pathway.

Cell Mol Gastroenterol Hepatol 2021 Jul 23. Epub 2021 Jul 23.

Department of Liver Surgery and Liver Transplantation Center, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200127, China.

Background & Aims: Sustained JNK activation plays a major role in drug-induced liver injury (DILI). Stress-responsive microRNA-31 (miR-31) has been implicated in regulating different cellular damage, and JNK activation could induce miR-31 expression. However, the regulatory role of miR-31 in DILI has not been previously studied. We aimed to investigate whether miR-31 could ameliorate DILI and ascertain potential molecular mechanism.

Methods: miR-31 gene knockout (31-KO) and wild-type (WT) C57BL/6J mice were used to construct APAP-induced DILI model. Primary mouse hepatocytes, as well as AML-12 cell lines were used for in vitro experiments. Ago2-associated RNA immunoprecipitation combined with high-throughput sequencing (RIP-seq) were performed to identify specific targets of miR-31.

Results: 31-KO mice exhibited higher mortality rate, liver transaminase levels and hepatic necrosis compared to those in WT mice after APAP-induced hepatotoxicity. The protective role of miR-31 on hepatocytes has been analyzed via constructing bone marrow chimeric mice. Mechanistically, we found that hepatic JNK phosphorylation increased significantly in 31-KO mice. This caused mitochondrial p-Src inactivation and more reactive oxygen species production, which directly amplifies hepatocytes necrotic cell death, while administration of JNK specific inhibitor SP600125 could abrogate the differences. Moreover, bioinformatics analysis of RIP-seq identified that GTPase Cdc42, the upstream molecule of JNK signaling, was the specific target of miR-31 and could form a miR-31/Cdc42/p-MLK3 negative feedback loop to restrict JNK over-activation. Clinically, both miR-31 and p-JNK were highly elevated in liver tissues of DILI patients with different etiologies.

Conclusions: miR-31 can down-regulate Cdc42 to restrict over-activation of ROS/JNK/mitochondria necrotic death loop in hepatocytes of APAP induced DILI, which might provide a new therapeutic target for alleviating JNK over-activation based liver injury.
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http://dx.doi.org/10.1016/j.jcmgh.2021.07.011DOI Listing
July 2021

Cardiac, Macro-, and Micro-Circulatory Abnormalities in Association With Individual Metabolic Syndrome Component: The Northern Shanghai Study.

Front Cardiovasc Med 2021 9;8:690521. Epub 2021 Jul 9.

Department of Cardiology, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai, China.

This study investigated the association of metabolic syndrome (MS) and its components with cardiac, macro-, and micro-circulatory abnormalities in an elderly Chinese population. This cross-sectional study was conducted using data for 1,958 participants from the Northern Shanghai Study aged over 65 years without a history of cardiovascular disease. MS was defined according to the National Cholesterol Education Program Adult Treatment Panel III in 2005 (NCEPIII 2005). Asymptomatic cardiovascular impairment parameters, including the left ventricle mass index (LVMI), peak transmitral pulsed Doppler velocity/early diastolic tissue Doppler velocity (E/Ea), carotid-femoral pulse wave velocity (cf-PWV), ankle-brachial index (ABI), carotid intima-media thickness (CIMT), arterial plaque, and urinary albumin-creatinine rate (UACR), were evaluated. LVMI, E/Ea, cf-PWV, and the proportion of UACR > 30 mg/g exhibited increasing trends while ABI exhibited a decreasing trend according to the number of MS components (all for trend < 0.01). Logistic regression analysis revealed that MS was significantly associated with LV hypertrophy (LVH), LV diastolic dysfunction, arteriosclerosis, and microalbuminuria (all < 0.001). Central obesity and high blood pressure were associated with all cardiovascular abnormalities (all < 0.05), whereas elevated plasma glucose was associated with arteriosclerosis and microalbuminuria (both < 0.001). In addition, high triglyceride levels were associated with microalbuminuria ( < 0.05). MS is significantly associated with cardiac, macro-, and micro-circulatory abnormalities in elderly Chinese. Moreover, the presence of individual MS components may have specific prognostic significance.
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http://dx.doi.org/10.3389/fcvm.2021.690521DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8298861PMC
July 2021

Association of Red Meat and Poultry Consumption With the Risk of Metabolic Syndrome: A Meta-Analysis of Prospective Cohort Studies.

Front Nutr 2021 8;8:691848. Epub 2021 Jul 8.

Department of Orthopedics, Xiangya Hospital, Central South University, Changsha, China.

This study aims to investigate the association of red meat (processed and unprocessed) and poultry consumption with the risk of metabolic syndrome (MetS). Prospective cohort studies on the association of red meat (processed and unprocessed) and poultry consumption with the risk of MetS were identified by comprehensive literature search in the PubMed, Web of Science, and Embase databases up to March 2021. The pooled relative risk (RR) of MetS with 95% CIs for the highest vs. lowest category of red meat or poultry consumption was extracted for meta-analysis. A total of nine prospective cohort studies were included in this study. Among them, eight studies were identified for red meat consumption. The overall multi-variable adjusted RR demonstrated that red meat consumption was associated with a higher risk of MetS (RR = 1.35, 95% CI: 1.13-1.62; = 0.001). Moreover, four and three studies were specifically related to processed and unprocessed red meat consumption, respectively. Both processed (RR = 1.48, 95% CI: 1.11-1.97; = 0.007) and unprocessed red meat (RR = 1.32, 95% CI: 1.14-1.54; = 0.0003) consumption was associated with a higher risk of MetS. With regard to poultry consumption, three studies were included. The overall multi-variable adjusted RR suggested that poultry consumption was associated with lower risk of MetS (RR = 0.85, 95% CI: 0.75-0.97; = 0.02). The current evidence indicates that red meat (processed and unprocessed) consumption is associated with a higher risk of MetS, whereas, poultry consumption is associated with a lower risk of MetS. More well-designed randomized controlled trials are still needed to address the issues further.
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http://dx.doi.org/10.3389/fnut.2021.691848DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8295459PMC
July 2021

The effect of the prospective information-based nursing quality management model on the improvement of management quality in emergency medicine nursing management.

Authors:
Qian Dong Yi Zhang

Am J Transl Res 2021 15;13(6):7406-7411. Epub 2021 Jun 15.

Department of Outpatient, Shandong Provincial Third Hospital Jinan 250031, Shandong Province, China.

Objective: This study aimed to explore the clinical effectiveness of applying a prospective information-based nursing quality management model in emergency medicine nursing management and its impact on the management quality.

Methods: 170 inpatients treated in the emergency department of our hospital from April 2018 to March 2019 were recruited as the study cohort and randomly divided into a control group and a study group, with 85 patients in each group. In the study, the patients in the control group were treated using the routine nursing mode, and the patients in the study group were treated using the prospective information-based nursing quality management model to compare the nursing management quality, the nursing satisfaction, the occurrence of adverse events, the nursing compliance, the emergency response times, and the survival rates between the two groups.

Results: We found that the overall nursing management quality in the study group was better than it was in the control group. The nursing satisfaction rate in the study group (96.47%) was higher than the nursing satisfaction rate in the control group (83.53%) The rate of adverse events in the study group (5.88%) was lower than it was in the control group (16.47%), the nursing compliance rate in the study group (89.41%) was higher than it was in the control group (63.53%), the emergency response times in the study group were shorter than they were in the control group, and the postoperative survival rate in the study group was higher than it was in the control group, all with statistically significant differences (P<0.05).

Conclusion: The prospective information-based nursing quality management model has a significant influence on emergency medicine nursing management. It can significantly improve the nursing management quality, the patients' satisfaction with the nursing process, and the nursing compliance, and it can effectively avoid adverse events, so it is worthy of promotion.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8290796PMC
June 2021

The Effects of Interferons on Allogeneic T Cell Response in GVHD: The Multifaced Biology and Epigenetic Regulations.

Front Immunol 2021 8;12:717540. Epub 2021 Jul 8.

Penn State Cancer Institute, Penn State University College of Medicine, Hershey, PA, United States.

Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is a potentially curative therapy for hematological malignancies. This beneficial effect is derived mainly from graft-versus-leukemia (GVL) effects mediated by alloreactive T cells. However, these alloreactive T cells can also induce graft-versus-host disease (GVHD), a life-threatening complication after allo-HSCT. Significant progress has been made in the dissociation of GVL effects from GVHD by modulating alloreactive T cell immunity. However, many factors may influence alloreactive T cell responses in the host undergoing allo-HSCT, including the interaction of alloreactive T cells with both donor and recipient hematopoietic cells and host non-hematopoietic tissues, cytokines, chemokines and inflammatory mediators. Interferons (IFNs), including type I IFNs and IFN-γ, primarily produced by monocytes, dendritic cells and T cells, play essential roles in regulating alloreactive T cell differentiation and function. Many studies have shown pleiotropic effects of IFNs on allogeneic T cell responses during GVH reaction. Epigenetic mechanisms, such as DNA methylation and histone modifications, are important to regulate IFNs' production and function during GVHD. In this review, we discuss recent findings from preclinical models and clinical studies that characterize T cell responses regulated by IFNs and epigenetic mechanisms, and further discuss pharmacological approaches that modulate epigenetic effects in the setting of allo-HSCT.
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http://dx.doi.org/10.3389/fimmu.2021.717540DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8297501PMC
July 2021

How physicians respond to negative emotions in high-risk preoperative conversations.

Patient Educ Couns 2021 Jul 9. Epub 2021 Jul 9.

The Third Xiangya Hospital of Central South University, Changsha, China.

Objective: To investigate physicians' responses to negative emotions in high-risk preoperative conversations; and to explore the influencing factors of these responses.

Methods: One hundred and sixty-two audio recordings were coded using the Chinese Verona Coding Definition of Emotional Sequences (VR-CoDES). Big Five Personality Inventory Brief Version and Emotional Intelligence Scale were administered to explore the influencing factors of physicians' responses. SPSS 24.0 and R 3.6.3 LME4 Package were used for data analysis.

Results: Reduce Space (83%), referring to physicians' responses reducing the opportunities of patients to disclose emotions, was physicians' most frequent response to patients or families' emotions. The main responses were Information-advice (ERIa) and Ignoring (NRIa). Younger age, female, Agreeableness and Openness were factors positively associated with Explicit Provide Space (EP); Neuroticism was negatively correlated with EP. Extroversion was negatively correlated with Explicit Reduce Space (ER); Conscientiousness was negatively correlated with both EP and ER responses. Emotional intelligence had no significant influence on physicians' responses.

Conclusion: The majority of physicians were inclined to reduce space by providing information advice or ignoring. Physicians' responses were correlated with their gender, age and personality traits.

Practice Implications: The trainees' gender, age and personality should be considered when conducting doctor-patient communication skills training.
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http://dx.doi.org/10.1016/j.pec.2021.06.022DOI Listing
July 2021

Treatment of oil-based drilling cuttings using the demulsification separation-Fenton oxidation method.

Environ Sci Pollut Res Int 2021 Jul 24. Epub 2021 Jul 24.

School of Resources and Environmental Engineering, Wuhan University of Technology, Wuhan, 430070, China.

In this study, demulsification separation-Fenton oxidation technology was employed as a combined technology to treat total petroleum hydrocarbons (TPH) in oil-based drill cuttings (OBDC). Batch experiments were carried out to optimize the technology parameter. Under the optimal condition, 70% and 51% TPH removal rate was obtained for demulsification technology and Fenton oxidation technology, respectively. Eighty-five percent of TPH removal rate was obtained using combination technology of demulsification separation and Fenton oxidation. Multiple characterizations were used to analyze the physical and chemical properties of treated OBDC. The result of XRD pattern indicated the combination technology had no obvious effect for structure phase of OBDC. The results of FTIR, GC-MS, TG-DTG and SEM were used to characterize the treated OBDC. This paper provides an efficient and feasible combined technology for OBDC treatment, which expands a new strategy for the removal of TPH from solid waste.
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http://dx.doi.org/10.1007/s11356-021-15509-7DOI Listing
July 2021

Verticillium dahliae Secretory Effector PevD1 Induces Leaf Senescence by Promoting ORE1-Mediated Ethylene Biosynthesis.

Mol Plant 2021 Jul 21. Epub 2021 Jul 21.

Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China;; Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education Institutes, Department of Biology, Southern University of Science and Technology (SUSTech), Shenzhen, Guangdong, 518055, China. Electronic address:

Leaf senescence, the final stage of leaf development, is influenced by numerous internal and environmental signals. So far, how biotic stresses such as pathogen infection regulate leaf senescence is unclear. Here, we found that the premature leaf senescence caused by a soil-borne vascular fungus Verticillium dahliae in Arabidopsis was impaired by the mutation of a protein elicitor from V. dahliae 1 (PevD1). Constitutive or inducible overexpression of PevD1 accelerated Arabidopsis leaf senescence. A senescence-associated NAC transcription factor, ORE1, was targeted by PevD1. PevD1 interacted with and stabilized ORE1 protein by disrupting its interaction with the RING-type ubiquitin E3 ligase NLA. Mutation of ORE1 suppressed the premature senescence caused by overexpressing PevD1. Overexpression of ORE1 or PevD1 led to enhanced ethylene production, and ORE1 mediated PevD1-induced ethylene biosynthesis by directly binding to the ACS6 promoter. Loss-of-function of ACSs suppressed V. dahliae-induced leaf senescence in ORE1-overexpressing plants. Interestingly, PevD1 also interacted with Gossypium hirsutum ORE1 (GhORE1), and virus-induced gene silencing of GhORE1 delayed V. dahliae-triggered leaf senescence in cotton, indicative of the existence of a conserved mechanism in plants. Altogether, our study demonstrates that V. dahliae induces leaf senescence by secreting the effector PevD1 to regulate the ORE1-ACS6 cascade, providing new insight into biotic stress-induced senescence in plants.
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http://dx.doi.org/10.1016/j.molp.2021.07.014DOI Listing
July 2021

Individualized Thalamic Parcellation Reveals Alterations in Shape and Microstructure of Thalamic Nuclei in Patients with Disorder of Consciousness.

Cereb Cortex Commun 2021 2;2(2):tgab024. Epub 2021 Apr 2.

Key Laboratory for Biomedical Engineering of Ministry of Education, College of Biomedical Engineering & Instrument Science, Zhejiang University, Hangzhou, 310027, P.R. China.

The thalamus plays crucial roles in consciousness generation and information processing. Previous evidence suggests that disorder of consciousness (DOC) caused by severe brain injury, is potentially related to thalamic abnormalities. However, how the morphology and microstructure change in thalamic subfields and thalamocortical fiber pathways in patients with DOC, and the relationships between these changes and the consciousness status remain unclear. Here, we generated the individual-specific thalamic parcellation in 10 DOC patients and 10 healthy controls (HC) via a novel thalamic segmentation framework based on the fiber orientation distribution (FOD) derived from 7-Tesla diffusion MRI, and investigated the shape deformation of thalamic nuclei as well as the microstructural changes associated with thalamic nuclei and thalamocortical pathways in patients with DOC. Enlargement of dorsal posterior nucleus and atrophy of anterior nucleus in the right thalamus were observed in DOC cohort relative to the HCs, and the former was closely linked to the consciousness level of the patients. We also found significant reductions of fiber density, but not fiber bundle cross-section, within several thalamic nuclei and most of the thalamocortical fiber pathways, suggesting that loss of axons might take primary responsibility for the impaired thalamocortical connections in patients with DOC rather than the change in fiber-bundle morphology. Furthermore, the individual-specific thalamic parcellation achieved 80% accuracy in classifying patients at the minimally conscious state from the vegetative state, compared with ~60% accuracy based on group-level parcellations. Our findings provide the first evidence for the shape deformation of thalamic nuclei in DOC patients and the microstructural basis of the disrupted thalamocortical connections.
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http://dx.doi.org/10.1093/texcom/tgab024DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8152869PMC
April 2021

AIRE-overexpressing BMDCs suppress T cells through ICOSL to prevent and attenuate autoimmune diabetes in NOD mice.

Int Immunopharmacol 2021 Jul 19;99:107979. Epub 2021 Jul 19.

Department of Immunology, College of Basic Medical Sciences, Jilin University, Changchun 130021, China. Electronic address:

The strong genetic association between autoimmune regulator (AIRE) and autoimmune diseases indicates its critical role in immune tolerance. AIRE deficiency is thought to promote the development of follicular helper T (T) cells, which are considered to be essential in B cell proliferation. Excessive T cell generation is a key step towards the development of autoimmune diseases, including type 1 diabetes. However, the potential mechanism by which AIRE contributes to the generation and function of the T cell population has remained elusive. We show that AIRE reduced T cell generation by inhibiting the expression of inducible costimulatory ligand (ICOSL), interleukin (IL)-6 and IL-27 in dendritic cells (DCs). To understand the precise impact of AIRE-overexpressing bone marrow-derived DCs (AIRE-BMDCs) on type 1 diabetes progression and the associated molecular mechanisms, we transferred AIRE-BMDCs to recipient NOD mice and found that transplantation of AIRE-BMDCs can prevent or delay the onset of diabetes, attenuate diabetes after the establishment of overt hyperglycaemia, and lead to the inhibition of autoreactive pathological T cells and germinal centre (GC) B cells. To further determine the potential mechanism underlying this T cell depletion, BMDCs were cotransferred with recombinant mouse ICOSL (ICOSLG protein). We demonstrated that NOD mice were more susceptible to diabetes when they received AIRE-BMDCs and ICOSLG than when they received only mock-vehicle BMDCs (GFP-BMDCs). In addition, we did not observe the reversal of diabetes in any mice subjected to this cotransfer system. A single cycle of ICOSLG treatment temporarily promoted T cell proliferation and GC development. Our results reveal a mechanistic role of AIRE-BMDCs in the initiation of T cell differentiation, and the AIRE-mediated decrease in ICOSL expression in BMDCs plays a critical role. The effect of decreased ICOSL expression in type 1 diabetes will guide the design and evaluation of parallel studies in patients.
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http://dx.doi.org/10.1016/j.intimp.2021.107979DOI Listing
July 2021

Clinical features and imaging manifestations of retinoblastoma with hepatic metastasis.

Pediatr Blood Cancer 2021 Jul 22:e28959. Epub 2021 Jul 22.

Department of Pediatrics, Beijing Tongren Hospital, Capital Medical University, Beijing, China.

Clinical data of five patients with hepatic metastases of retinoblastoma were analyzed retrospectively (two had bilateral tumors three had unilateral intraocular tumors). On computed tomography, multiple and single low-density foci were observed. Four patients had tumor remission, and one showed no response after chemotherapy. Three patients who underwent enucleation were at high risk for extensive choroidal invasion. Central nervous system and bone metastases occurred in all five patients. Neuron-specific enolase and lactate dehydrogenase levels were significantly elevated in all patients. Two patients died (not from hepatic metastasis). Three patients (one with tumor progression and two with shorter courses) are continuing treatment.
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http://dx.doi.org/10.1002/pbc.28959DOI Listing
July 2021

A High-Throughput Nanofluidic Device for Exosome Nanoporation to Develop Cargo Delivery Vehicles.

Small 2021 Jul 21:e2102150. Epub 2021 Jul 21.

Laboratory of Biomedical Microsystems and Nano Devices, Center for Bionic Sensing and Intelligence, Institute of Biomedical and Health Engineering, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China.

Efficient loading of various exogenous cargos into exosomes while not affecting their integrity and functionalities remains a major challenge. Here, a nanofluidic device named "exosome nanoporator (ENP)" is presented for high-throughput loading of various cargos into exosomes. By transporting exosomes through nanochannels with height comparable to their dimension, exosome membranes are permeabilized by mechanical compression and fluid shear, allowing the influx of cargo molecules into the exosomes from the surrounding solution while maintaining exosome integrity. The ENP consisting of an array of 30 000 nanochannels demonstrates a high sample throughput, and the working mechanism of the device is elucidated through experimental and numerical study. Further, the exosomes treated by the ENP can deliver their drug cargos to human non-small cell lung cancer cells and induce cell death, indicating the potential opportunities of the device for developing new exosome-based delivery vehicles for medical and biological applications.
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http://dx.doi.org/10.1002/smll.202102150DOI Listing
July 2021

CXCL11 Signaling in the Tumor Microenvironment.

Authors:
Qun Gao Yi Zhang

Adv Exp Med Biol 2021 ;1302:41-50

Biotherapy Center and Cancer Center, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, People's Republic of China.

CXCL11 which can bind to two different chemokine receptors, CXCR3 and CXCR7, has found a prominent place in current tumor research. In this chapter, we mainly discuss the current evidence on the role of the immune response of CXCL11 in tumor microenvironment (TME). The diverse functions of CXCL11 include inhibiting angiogenesis, affecting the proliferation of different cell types, playing a role in fibroblast directed carcinoma invasion, increasing adhesion properties, suppressing M2 macrophage polarization, and facilitating the migration of certain immune cells. In addition, we discussed the application of CXCL11 as an adjuvant to various mainstream anti-cancer therapies and the future challenges in the application of CXCL11 targeted therapies.
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http://dx.doi.org/10.1007/978-3-030-62658-7_4DOI Listing
July 2021

Molecular Mechanism of Polymyxin Resistance in Multidrug-Resistant and Isolates from Henan Province, China: A Multicenter Study.

Infect Drug Resist 2021 12;14:2657-2666. Epub 2021 Jul 12.

Department of Clinical Microbiology, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, People's Hospital of Henan University, Zhengzhou, Henan, People's Republic of China.

Purpose: To evaluate polymyxin-resistant and prevalence and characteristics in the Henan province, China.

Materials And Methods: A total of 2301 bacterial isolates collected at six hospitals were assessed. Their response to polymyxin was evaluated by minimum inhibitory concentration (MIC) analysis, and the mobilized colistin resistance (mcr) and carbapenemase gene were explored. Mutations on , and in polymyxin-resistant were detected by PCR. , and transcriptional levels were quantified by RT-qPCR. Pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing were performed to determine the phylogenetic relationship between the polymyxin-resistant isolates.

Results: Of the and isolates identified, 0.3% and 1.4% were polymyxin-resistant, respectively, with MICs of 4-64 μg/mL. All polymyxin-resistant isolates were susceptible to tigecycline. Four isolates were -positive and one was carbapenem-resistant, carrying and . One isolate was -positive and nine were carbapenem-resistant (PRCRKP), carrying but not . The five isolates belonged to four sequence types (ST2, ST132, ST632, and ST983). All PRCRKP isolates belonged to ST11. However, all 16 isolates belonged to different PFGE types with <95% genetic similarity. Insertion sequences in were detected in nine (81.8%) polymyxin-resistant samples. Colistin resistance was linked with operon upregulation, with , and being overexpressed in all but one of the polymyxin-resistant samples. Furthermore, 33.3% of patients carrying polymyxin-resistant isolates had previously used polymyxin, and 66.7% patients displayed good clinical outcomes.

Conclusion: The polymyxin resistance rate was slightly higher than that of and was more common in than in . Moreover, the insertion of ISkpn14 into may be the main contributor to polymyxin-resistance in in Henan.
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http://dx.doi.org/10.2147/IDR.S314490DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8285567PMC
July 2021

Decreased SFRP5 correlated with excessive metabolic inflammation in polycystic ovary syndrome could be reversed by metformin: implication of its role in dysregulated metabolism.

J Ovarian Res 2021 Jul 20;14(1):97. Epub 2021 Jul 20.

Reproductive Medicine Center, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, People's Republic of China.

Background: Polycystic Ovary Syndrome (PCOS) is a complex endocrine disorder of heterogeneous nature. Secreted frizzled-related protein (SFRP) 5 is an anti-inflammatory adipokine implicated in metabolic homeostasis. We aimed to confirm the correlation between SFRP5, metabolic inflammation and PCOS, investigate the predictive value of SFRP5 for PCOS and the involvement of SFRP5 in metformin treated PCOS.

Methods: This retrospective case-control study included 140 PCOS and 33 control women. Sixty seven PCOS women were included for detecting serum SFRP5 level and its correlation with metabolic inflammation. Predictive value of SFRP5 for PCOS was evaluated by logistic regression and receiver operating characteristic (ROC) analyses. Seventy three PCOS women complicated with impaired glucose tolerance (IGT)/insulin resistance (IR) were included for investigating the effects of metformin (37 with metformin vs. 36 without metformin) on SFRP5, pro-inflammatory cytokines, ovulation and pregnancy rate.

Results: Plasma SFRP5 levels were decreased in PCOS (odds ratio: 0.78, 95% confidence interval (CI):0.703-0.866, P < 0.001) independent of obesity. SFRP5 was negatively associated with IL-6, TNFα, FAI and HOMA-IR. The cut-off point of SFRP5 < 46.13 ng/ml was optimal to identify PCOS with a higher specificity of 96.87% and a relatively lower sensitivity compared to AMH. SFRP5 increased specificity of AMH for predicting PCOS, especially which with relatively decreased AMH (< 4.7 ng/ml). Metformin promoted SFRP5 and decreased leptin, IL-6 and TNFα secretion in PCOS women with metabolic abnormality in a time dependent manner and with improved ovulation rate and pregnancy rate.

Conclusion: Decreased SFRP5 was associated with metabolic inflammation in PCOS and has a potential role for the supplement of AMH in predicting PCOS. The reverse of serum SFRP5 by metformin indicated that SFRP5 participated in the improvment of follicular development by metformin. Further prospective investigations are needed to confirm these preliminary data.
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http://dx.doi.org/10.1186/s13048-021-00847-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8293500PMC
July 2021

US Contrast Agent Arrival Time Difference Ratio for Benign versus Malignant Subpleural Pulmonary Lesions.

Radiology 2021 Jul 20:204642. Epub 2021 Jul 20.

From the Department of Ultrasound, Shanghai Pulmonary Hospital, Tongji University School of Medicine, No. 507 Zhengmin Rd, Shanghai 200433, China (K.B., R.R.Z., Y.Z., M.J.S., H.W.C., Y.C., H.M.Z., C.H.T., J.Y., Y.W.); and Department of Pathology, Tongji Hospital, Tongji University School of Medicine, Shanghai, China (K.B.).

Background US has proven valuable in the diagnosis of subpleural pulmonary lesions (SPLs); however, existing US indicators have limitations. Purpose To propose and validate a revised contrast-enhanced (CE) US indicator for differential diagnosis of benign and malignant SPLs and to compare its performance with existing CE US diagnostic criteria. Materials and Methods This prospective study (Chinese clinical trial registry, ChiCTR1800019828) enrolled patients with SPLs between May 2019 and August 2020. They were divided into a developmental cohort (DC) and a validation cohort (VC). In the DC, the optimal indicator was selected from five CE US indicators. In the VC, the selected indicator was compared with existing CE US diagnostic criteria using the area under the receiver operating characteristic curve (AUC). Pathologic analysis, microbial evidence, and clinical follow-up were used as reference standards for all SPLs. Results A total of 902 participants (DC, 424 participants; VC, 478 participants) with SPLs (mean age, 56 years ± 17; 593 men) were evaluated. The arrival time (AT) difference ratio proved to be the optimal indicator to distinguish benign from malignant SPLs. In the overall (regardless of lesion size), large (vertical diameter >3 cm), and small (vertical diameter ≤3 cm) lesion groups, the cutoff values of the AT difference ratio were 43%, 42%, and 50% and the AUCs obtained from the VC were 0.91 (95% CI: 0.88, 0.93), 0.97 (95% CI: 0.94, 0.98), and 0.77 (95% CI: 0.71, 0.83) respectively, which were higher than those of lesion-lung AT difference greater than 2.5 seconds (0.81 [ < .001], 0.85 [ < .001], and 0.7 [ = .005], respectively), lesion AT greater than 7.5 seconds (0.65 [ < .001], 0.64 [ < .001], and 0.63 [ < .001], respectively), and lesion AT greater than 10 seconds (0.67 [ < .001], 0.68 [ < .001], and 0.64 [ < .001] respectively). Conclusion The US contrast agent arrival time difference ratio enables better differentiation of benign and malignant subpleural lesions when compared with existing diagnostic criteria. . Published under a CC BY 4.0 license.
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http://dx.doi.org/10.1148/radiol.2021204642DOI Listing
July 2021

Hydrogen regulates the M1/M2 polarization of alveolar macrophages in a rat model of chronic obstructive pulmonary disease.

Exp Lung Res 2021 Jul 20:1-10. Epub 2021 Jul 20.

College of Integrated Chinese and Western Medicine, Anhui University of Chinese Medicine, Hefei, Anhui, China.

Objective: Chronic obstructive pulmonary disease (COPD) is a respiratory disease with high morbidity and mortality worldwide, so far there is no ideal treatment method. Previous studies have shown that hydrogen (H) is involved in the treatment of COPD as an antioxidant. In this study, the effect of H on M1/M2 polarization of alveolar macrophages in COPD rats was observed, and its anti-inflammatory mechanism was further elucidated. : Twenty-four Sprague-Dawley rats were randomly divided into three groups including the control, COPD and H group. A rat model of COPD was established by cigarette exposure combined with lipopolysaccharide (LPS) induction. H therapy was administered 2 hours per day for 14 days. Lung function and pathology were assessed. The levels of interleukin (IL)-6, tumor necrosis factor (TNF)-α, transforming growth factor (TGF)-β1 and IL-10 in bronchoalveolar lavage fluid (BALF) and lung tissue were measured by enzyme-linked immunosorbent assay. The mRNA, protein expression and immunoreactivity of inducible nitric oxide synthase (iNOS) and arginase (Arg)-1 in lung were observed by quantitative real-time PCR, western blot and immunohistochemistry. : Compared with the control rats, there were a significant decline in lung function, a marked inflammatory infiltration and pulmonary parenchymal remodeling and the increases of IL-6, TNF-α and TGF-β1 levels in BALF and lung tissue, but a lower expression of IL-10 in COPD rats. The iNOS mRNA and protein expression, as well as its optical density (OD), were increased significantly in lung tissue, while those of Arg-1 decreased significantly. H treatment improved the lung function and the parenchymal inflammation, reversed the increased levels of IL-6, TNF-α and TGF-β1, and the lower IL-10. Meanwhile, H also down-regulated the expression of iNOS, but up-regulated expression of Arg-1 in lung tissue. : H reduces inflammation in the lung of COPD, which may be related to its inhibition of M1 type polarization and activation of M2 type polarization of alveolar macrophage.
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http://dx.doi.org/10.1080/01902148.2021.1919788DOI Listing
July 2021
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