Publications by authors named "Zhang Wentao"

285 Publications

A photoaffinity labeling strategy identified EF1A1 as a binding protein of cyclic dinucleotide 2'3'-cGAMP.

Cell Chem Biol 2021 Aug 30. Epub 2021 Aug 30.

Interdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 201210, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

2'3'-cyclic GMP-AMP (2'3'-cGAMP), generated by cyclic GMP-AMP synthase (cGAS) under activation by cytosolic DNA, has a vital role in innate immune response via its receptor protein stimulator of interferon genes (STING) to fight viral infections and tumors. In order to have a complete understanding of biological functions of 2'3'-cGAMP, it is important to find out whether 2'3'-cGAMP has other unrevealed binding proteins present in mammalian cells and executes unknown functions. Here we report the 2'3'-cGAMP-based photoaffinity probes that capture and isolate 2'3'-cGAMP-binding proteins. These probes enable the identification of some potential 2'3'-cGAMP-binding proteins from HeLa cells. EF1A1, an essential protein regulating protein synthesis, is further validated to associate with 2'3'-cGAMP in vitro and in cells to impede protein synthesis. Thus, our studies provide a powerful approach to enable identification of the 2'3'-cGAMP interactome, discover unknown functions of 2'3'-cGAMP, and understand its physiological/pathological roles in tumor immunity and immune-related diseases.
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http://dx.doi.org/10.1016/j.chembiol.2021.08.006DOI Listing
August 2021

Evaluation of Genomic Prediction for Fusarium Head Blight Resistance with a Multi-Parental Population.

Biology (Basel) 2021 Aug 6;10(8). Epub 2021 Aug 6.

Aquatic and Crop Resources Development, National Research Council of Canada, Ottawa, ON K1A 0R6, Canada.

Fusarium head blight (FHB) resistance is quantitatively inherited, controlled by multiple minor effect genes, and highly affected by the interaction of genotype and environment. This makes genomic selection (GS) that uses genome-wide molecular marker data to predict the genetic breeding value as a promising approach to select superior lines with better resistance. However, various factors can affect accuracies of GS and better understanding how these factors affect GS accuracies could ensure the success of applying GS to improve FHB resistance in wheat. In this study, we performed a comprehensive evaluation of factors that affect GS accuracies with a multi-parental population designed for FHB resistance. We found larger sample sizes could get better accuracies. Training population designed by CDmean based optimization algorithms significantly increased accuracies than random sampling approach, while mean of predictor error variance (PEVmean) had the poorest performance. Different genomic selection models performed similarly for accuracies. Including prior known large effect quantitative trait loci (QTL) as fixed effect into the GS model considerably improved the predictability. Multi-traits models had almost no effects, while the multi-environment model outperformed the single environment model for prediction across different environments. By comparing within and across family prediction, better accuracies were obtained with the training population more closely related to the testing population. However, achieving good accuracies for GS prediction across populations is still a challenging issue for GS application.
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http://dx.doi.org/10.3390/biology10080756DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8389552PMC
August 2021

Two-Dimensional Boron-Rich Monolayer BN as High Capacity for Lithium-Ion Batteries: A First-Principles Study.

ACS Appl Mater Interfaces 2021 Sep 23;13(34):41169-41181. Epub 2021 Aug 23.

School of Materials Science and Engineering, Xi'an University of Science and Technology, Xi'an 710054, China.

Owing to lightweight, abundant reserves, low cost, and nontoxicity, B-based two-dimensional (2D) materials, e.g., borophene, exhibit great potential as new anode materials with higher energy density for Li-ion batteries (LIBs). However, exfoliation of borophene from the Ag substrate remains the most daunting challenge due to their strong interfacial interactions, significantly restricting its practical applications. In this study, through first-principles swarm-intelligence structure calculations, we have found several Boron-rich boron nitride BN materials ( = 2, 3, 4, and 5) with increased stability and weakened interactions with the Ag(111) substrate compared with δ6-borophene. A high cohesive energy and superior dynamical, thermodynamic, and mechanical stability provide strong feasibility for their experimental synthesis. The obtained BN materials exhibit a high mechanical strength (94-226 N/m) and low interfacial bonding with the Ag substrate, from -0.043 to -0.054 eV Å, significantly smaller than that of δ6-borophene. Among them, BN and BN exhibit not only a remarkably high storage capacity of 1805-3153 mAh/g but also a low barrier energy and open-circuit voltage. Moreover, BN showed a cross-sheet motion with a low barrier of 0.24 eV, which is unique compared with the in-plane diffusion in most other 2D electrode materials restricted by their quasi-flat geometry. BN also exhibits excellent cyclability with improved metallic conductivity upon Li-ion intercalation, showing great potential in LIB applications. This study opens up a new avenue to explore B-rich 2D electrode materials in energy applications and provide instructive insights into borophene functionalization and exfoliation.
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http://dx.doi.org/10.1021/acsami.1c08331DOI Listing
September 2021

1-Methylcyclopropene (1-MCP) retards the senescence of by regulating the cellular energy status and membrane lipid metabolism.

Food Sci Nutr 2021 Aug 23;9(8):4349-4363. Epub 2021 Jun 23.

College of Food Science Northeast Agricultural University Harbin PR China.

1-MCP is an ethylene inhibitor which can delay the ripening and senescence of fruits and vegetables effectively. (PA) is one of the wild vegetables which is famous and nutrient in China. However, the mechanism of PA preservation treated with 1-MCP has not been reported. Consequently, the effects of postharvest 1-MCP treatment on the changes in quality, energy metabolism, and membrane lipid metabolism of PA were investigated in this study. The results indicated that 1-MCP treatment could effectively inhibit the decreases in firmness, titratable acid (TA) content and the increases in weight loss rate, malondialdehyde (MDA) content, membrane permeability, and membrane lipid metabolism-related enzymes in PA. The cellular energy charge (EC) and the levels of ATP, ATP/ADP, and ATP/AMP, the activities of energy metabolism-related enzymes, NAD, and NADH were maintained, and the decreases in unsaturated fatty acids and the ratio of unsaturated-to-saturated fatty acids in the membrane of PA cells were effectively retarded by 1-MCP treatment. A positive correlation was observed between cellular ATP levels and the ratio of unsaturated-to-saturated fatty acids, while negative correlations were observed between the ratio of unsaturated-to-saturated fatty acids and both lipid peroxidation and membrane permeability. These results indicated that higher levels of energy status, unsaturated-to-saturated fatty acid ratios, and lipid metabolism in the membrane could preserve the membrane integrity of postharvest PA and effectively extend its shelf life.
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http://dx.doi.org/10.1002/fsn3.2406DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8358344PMC
August 2021

Strategies for Browning Agent Delivery.

Pharm Res 2021 Aug 16;38(8):1327-1334. Epub 2021 Aug 16.

College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, 310058, China.

Obesity expands as a global climbing epidemic that is often correlated to cardiovascular diseases and endocrine disorders. Converting white adipocytes to brown adipocytes for enhanced energy expenditure has recently emerged as a promising anti-obesity treatment. However, the conventional approaches to apply browning agents systematically suffer from off-target effects, multiple dosage requirements, and poor patient compliance. To date, various delivery strategies have been reported to deliver browning agents for obesity treatment in a safer and more controllable manner. This review will discuss the latest designs in browning agent delivery systems with a focus on nanomedicines and transdermal patches.
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http://dx.doi.org/10.1007/s11095-021-03081-1DOI Listing
August 2021

[Interpretation of 2020 American Academy of Orthopaedic Surgeons (AAOS) on the Management of Glenohumeral Joint Osteoarthritis Evidence-Based Clinical Practice Guideline].

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2021 Aug;35(8):935-940

Center of Sports Medicine and Rehabilitation, Peking University Shenzhen Hospital, Shenzhen Guangdong, 518000, P.R.China.

The 2020 Management of Glenohumeral Joint Osteoarthritis Evidence-Based Clinical Practice Guideline which was prepared by the American Academy of Orthopaedic Surgeons (AAOS) were publicated on October 2020. The guideline involves the following 8 chapters: drug therapy and injectable biologics, physical therapy and non-surgical treatments, radiographs, prognostic factors, surgical treatments, intraoperative hemostasis measure (tranexamic acid), management of supraspinatus tears, multimodal pain management and discharge. In this paper, the guideline is interpreted to provide cutting-edge information for domestic glenohumeral joint osteoarthritis researchers.
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http://dx.doi.org/10.7507/1002-1892.202103088DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8404005PMC
August 2021

Microneedle-Mediated Vaccination: Innovation and Translation.

Adv Drug Deliv Rev 2021 Aug 8:113919. Epub 2021 Aug 8.

College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China; Department of General Surgery, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, China; Zhejiang Laboratory of Systems & Precision Medicine, Zhejiang University Medical Center, Hangzhou 311121, China; MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China. Electronic address:

Vaccine administration by subcutaneous or intramuscular injection is the most commonly prescribed route for inoculation, however, it is often associated with some deficiencies such as low compliance, high professionalism, and risk of infection. Therefore, the application of microneedles for vaccine delivery has gained widespread interests in the past few years due to its high compliance, minimal invasiveness, and convenience. This review focuses on recent advances in the development and application of microneedles for vaccination based on different delivery strategies, and introduces the current status of microneedle-mediated vaccination in clinical translation. The prospects for its application including opportunities and challenges are further discussed.
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http://dx.doi.org/10.1016/j.addr.2021.113919DOI Listing
August 2021

Synergistic Antioxidant Effects of Araloside A and L-Ascorbic Acid on HO-Induced HEK293 Cells: Regulation of Cellular Antioxidant Status.

Oxid Med Cell Longev 2021 9;2021:9996040. Epub 2021 Jul 9.

College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang 150030, China.

Araloside A is a pentacyclic triterpenoid saponin, and L-ascorbic acid is a globally recognized antioxidant. In this study, coadministered araloside A and L-ascorbic acid were found to have a strong synergistic antioxidant effect, and correlations between cellular antioxidant indexes and free radical scavenging ability were found. Individual and combined pretreatment with araloside A and L-ascorbic acid increased both cell viability and antioxidant enzyme activity and inhibited the release of lactate dehydrogenase (LDH); the accumulation of malondialdehyde (MDA), lipid peroxidation (LPO) products, and HO; and the production of intracellular reactive oxygen species (ROS), protein carbonyls, and 8-hydroxy-2-deoxy guanosine (8-OHdG). Free radical scavenging ability was positively correlated with superoxide dismutase (SOD) and catalase (CAT) activity, the glutathione (GSH)/oxidized glutathione (GSSG) ratio, and total antioxidant capacity (T-AOC). Our study is the first investigation of araloside A and L-ascorbic acid coadministration for the treatment of diseases caused by oxidative stress. The synergistic antioxidant effects of araloside A and L-ascorbic acid support their potential as functional food ingredients for the elimination of oxidative stress-induced adverse reactions.
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http://dx.doi.org/10.1155/2021/9996040DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8289608PMC
July 2021

Deep Learning-Based Magnetic Resonance Imaging Image Features for Diagnosis of Anterior Cruciate Ligament Injury.

J Healthc Eng 2021 2;2021:4076175. Epub 2021 Jul 2.

Department of Sports Medicine and Rehabilitation, Peking University Shenzhen Hospital, Shenzhen 518036, China.

To study and explore the adoption value of magnetic resonance imaging (MRI) in the diagnosis of anterior cruciate ligament (ACL) injuries, a multimodal feature fusion model based on deep learning was proposed for MRI diagnosis. After the related performance of the proposed algorithm was evaluated, it was utilized in the diagnosis of knee joint injuries. Thirty patients with knee joint injuries who came to our hospital for treatment were selected, and all patients were diagnosed with MRI based on deep learning multimodal feature fusion model (MRI group) and arthroscopy (arthroscopy group). The results showed that deep learning-based MRI sagittal plane detection had a great advantage and a high accuracy of 96.28% in the prediction task of ACL tearing. The sensitivity, specificity, and accuracy of MRI in the diagnosis of ACL injury was 96.78%, 90.62%, and 92.17%, respectively, and there was no considerable difference in contrast to the results obtained through arthroscopy ( > 0.05). The positive rate of acute ACL patients with bone contusion and medial collateral ligament injury was substantially superior to that of chronic injury. Moreover, the incidence of chronic injury ACL injury with meniscus tear and cartilage injury was notably higher than that of acute injury, with remarkable differences ( < 0.05). In summary, MRI images based on deep learning improved the sensitivity, specificity, and accuracy of ACL injury diagnosis and can accurately determined the type of ACL injury. In addition, it can provide reference information for clinical treatment plan selection and surgery and can be applied and promoted in clinical diagnosis.
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http://dx.doi.org/10.1155/2021/4076175DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8272672PMC
July 2021

Enhanced functional properties of chitosan films incorporated with curcumin-loaded hollow graphitic carbon nitride nanoparticles for bananas preservation.

Food Chem 2022 Jan 5;366:130539. Epub 2021 Jul 5.

College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China. Electronic address:

The exploration of novel functional packaging films is of great scientific and technological interest. Herein, a novel chitosan/hollow g-CN/curcumin (CS-HCNS-Cur) biocomposite films was successful fabricated with integrated functions of slow release, antimicrobial activity and food freshness preservation. CS-HCNS-Cur films take the advantages of the excellent thermal stability and slow-release ability of HCNS to curcumin. Among the characterizations including scanning electron microscopy, transmission electron microscope, atomic force microscopy, fourier transform infrared spectroscopy, mechanical properties and the rheological properties measurements confirmed the successful fabrication of CS-HCNS-Cur films. The averaged water contact angle and water vapor permeability of this film were 105.83° and 105.03 × 10 g·mm (m·h·kPa), respectively. This film showed pH-responsive and slow-release ability. Moreover, this film can effectively store bananas for 10 days. Therefore, CS-HCNS-Cur films have promising potential for applications in functional food packaging.
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http://dx.doi.org/10.1016/j.foodchem.2021.130539DOI Listing
January 2022

Construction and Validation of a Novel Eight-Gene Risk Signature to Predict the Progression and Prognosis of Bladder Cancer.

Front Oncol 2021 29;11:632459. Epub 2021 Jun 29.

Department of Urology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China.

The progression from non-muscle-invasive bladder cancer (NMIBC) to muscle-invasive bladder cancer (MIBC) increases the risk of death. It is therefore important to find new relevant molecular models that will allow for effective prediction of the progression and prognosis of bladder cancer (BC). Using RNA-Sequence data of 49 BC patients in Shanghai tenth people's hospital (STPH) and weighted gene co-expression network analysis methods, a co-expression network of genes was developed and three key modules associated with malignant progression were selected. Based on the genes in three key modules, an eight-gene risk signature was established using univariate Cox regression and the Least absolute shrinkage and selection operator Cox model in The Cancer Genome Atlas Program (TCGA) and validated in validation sets. Subsequently, a nomogram based on the risk signature was constructed for prognostic prediction. The mRNA and protein expression levels of eight genes in cell lines and tissues were further investigated. The novel eight-gene risk signature was closely related to the malignant clinical features of BC and could predict the prognosis of patients in the training dataset (TCGA) and four validation sets (GSE32894, GSE13507, IMvigor210 trial, and STPH). The nomogram showed good prognostic prediction and calibration. The mRNA and protein expression levels of the eight genes were differentially expressed in cell lines and tissues. In our study, we established a novel eight-gene risk signature that could predict the progression and prognoses of BC patients.
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http://dx.doi.org/10.3389/fonc.2021.632459DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8276675PMC
June 2021

Photothermal-boosted effect of binary CuFe bimetallic magnetic MOF heterojunction for high-performance photo-Fenton degradation of organic pollutants.

Sci Total Environ 2021 Nov 5;795:148883. Epub 2021 Jul 5.

College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, PR China. Electronic address:

Overcoming the relatively low catalytic activity and strict acid pH condition of common photo-Fenton reaction is the key to alleviate the serious global burden caused by common organic pollutants. Herein, a binary homologous bimetallic heterojunction of magnetic [email protected](Fe, Cu) metal-organic frameworks (MCuFe MOF) with photothermal-boosted photo-Fenton activity is constructed as an ideal practical photo-Fenton catalyst for the degradation of organic pollutants. Through an in-situ derivation strategy, the formed homologous bimetallic heterojunction with binary redox couples can simultaneously improve the visible light harvesting capacity and expedite the separation and transfer of photogenerated electrons/holes pairs, leading to the continuous and rapid circulation of both Fe/Fe and Cu/Cu redox couples. Notably, the heterojunction shows intrinsic photo-thermal conversion effect, which is found to be beneficial to boost the photo-Fenton activity. Impressively, MCuFe MOF shows remarkable catalytic performance towards the degradation of various organic pollutants by comprehensively increasing HO decomposition efficiency and decreasing the required dosage of MCuFe MOF (0.05 g L) with a wide pH range (3.0-10.0). As such, a photo-Fenton catalyst consisting of binary homologous bimetallic heterojunction is first disclosed, as well as its photothermal-enhanced effect, which is expected to drive great advance in the degradation of organic pollutants for practical applications.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148883DOI Listing
November 2021

A naturally derived small molecule PSM0537 targets the AF1Q-TCF4 interaction to suppress COX2 expression and inhibit cell proliferation and metastasis in osteosarcoma.

Am J Cancer Res 2021 15;11(6):2637-2653. Epub 2021 Jun 15.

Department of Orthopaedics, The First Affiliated Hospital of Xi'an Jiaotong University Xi'an 710061, Shaanxi, China.

ALL1 fused gene from chromosome 1q (AF1Q) functions as an oncogene in several types of cancers, but it has not been observed in osteosarcoma. In this study, we revealed that AF1Q was overexpressed in multiple osteosarcoma cell lines, and its expression level increased with the severity of tumor malignancy in osteosarcoma biopsies. AF1Q was coupled with the transcription factor T cell factor 4 (TCF4) to assemble a complex to bind to the promoter of cyclooxygenase 2 () and activate its expression. The individual knockdown of , or in osteosarcoma cell lines significantly decreased cell proliferation and invasion . The tumor xenograft model also indicated that the individual knockdown of , or could inhibit tumor growth and metastasis. On the basis of these promising results, we established an AlphaScreen method to identify the compounds that disrupted the AF1Q-TCF4 interaction in a naturally derived small molecule pool. We discovered a compound called PSM0537, which showed a strong ability to inhibit the AF1Q-TCF4 interaction at a low dose of half-maximal inhibitory concentration (IC) (210.3 ± 15.6 nM). The administration of PSM0537 and could dramatically inhibit cell proliferation, invasion, and metastasis. Collectively, our findings reveal that the AF1Q-TCF4 transcriptional complex controls the expression of and that targeting the AF1Q-TCF4 interaction with PSM0537 could inhibit tumor cell growth and metastasis. Our results provide a new path for chemotherapy of osteosarcoma.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8263654PMC
June 2021

Optical manipulation of electronic dimensionality in a quantum material.

Nature 2021 07 7;595(7866):239-244. Epub 2021 Jul 7.

Key Laboratory of Artificial Structures and Quantum Control (Ministry of Education), Shenyang National Laboratory for Materials Science, School of Physics and Astronomy, Shanghai Jiao Tong University, Shanghai, China.

Exotic phenomena can be achieved in quantum materials by confining electronic states into two dimensions. For example, relativistic fermions are realized in a single layer of carbon atoms, the quantized Hall effect can result from two-dimensional (2D) systems, and the superconducting transition temperature can be considerably increased in a one-atomic-layer material. Ordinarily, a 2D electronic system can be obtained by exfoliating the layered materials, growing monolayer materials on substrates, or establishing interfaces between different materials. Here we use femtosecond infrared laser pulses to invert the periodic lattice distortion sectionally in a three-dimensional (3D) charge density wave material (1T-TiSe), creating macroscopic domain walls of transient 2D ordered electronic states with unusual properties. The corresponding ultrafast electronic and lattice dynamics are captured by time-resolved and angle-resolved photoemission spectroscopy and ultrafast electron diffraction at energies of the order of megaelectronvolts. Moreover, in the photoinduced 2D domain wall near the surface we identify a phase with enhanced density of states and signatures of potential opening of an energy gap near the Fermi energy. Such optical modulation of atomic motion is an alternative path towards realizing 2D electronic states and will be a useful platform upon which novel phases in quantum materials may be discovered.
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http://dx.doi.org/10.1038/s41586-021-03643-8DOI Listing
July 2021

Error Fusion of Hybrid Neural Networks for Mechanical Condition Dynamic Prediction.

Sensors (Basel) 2021 Jun 11;21(12). Epub 2021 Jun 11.

School of Mechanical Engineering, Dongguan University of Technology, Dongguan 523808, China.

It is important for equipment to operate safely and reliably so that the working state of mechanical parts pushes forward an immense influence. Therefore, in order to enhance the dependability and security of mechanical equipment, to accurately predict the changing trend of mechanical components in advance plays a significant role. This paper introduces a novel condition prediction method, named error fusion of hybrid neural networks (EFHNN), by combining the error fusion of multiple sparse auto-encoders with convolutional neural networks for predicting the mechanical condition. First, to improve prediction accuracy, we can use the error fusion of multiple sparse auto-encoders to collect multi-feature information, and obtain a trend curve representing machine condition as well as a threshold line that can indicate the beginning of mechanical failure by computing the square prediction error (). Then, convolutional neural networks predict the state of the machine according to the original data when the value exceeds the threshold line. It can be seen from this result that the EFHNN method in the prediction of mechanical fault time series is available and superior.
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http://dx.doi.org/10.3390/s21124043DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8230754PMC
June 2021

Can Smoking Cause Differences in Urine Microbiome in Male Patients With Bladder Cancer? A Retrospective Study.

Front Oncol 2021 8;11:677605. Epub 2021 Jun 8.

Department of Urology, Shanghai Tenth People's Hospital, Tongji University, Shanghai, China.

Background: Tobacco smoking is a carcinogen for many cancers including bladder cancer. The microbiota is involved in the occurrence, development, and treatment of tumors. We explored the composition of male urinary microbiome and the correlation between tobacco smoking and microbiome in this study.

Methods: Alpha diversity, principal component analysis (PCA) and Adonis analysis, linear discriminant analysis (LDA) coupled with effect size measurement, and PICRUSt function predictive analysis were used to compare different microbiome between smokers and non-smokers in men.

Results: There were 26 qualified samples included in the study. Eleven of them are healthy controls, and the others are from men with bladder cancer. Simpson index and the result of PCA analysis between smokers and non-smokers were not different (P > 0.05) in healthy men. However, the abundance of Bacteroidaceae, Erysipelotrichales, Lachnospiraceae, Bacteroides, and so on in the urinary tract of smokers is much higher than that of non-smokers. Compared to non-smokers, the alpha diversity in smokers was elevated in patients with bladder cancer (P < 0.05). PCA analysis showed a significant difference between smokers and non-smokers (P < 0.001), indicating that tobacco smoking plays a vital role in urinary tract microbial composition.

Conclusion: The composition of microbiome in the urinary tract is closely related to tobacco smoking. This phenomenon is more significant in patients with bladder cancer. This indicates tobacco smoking may promote the occurrence and development of bladder cancer by changing urinary tract microbiome.
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http://dx.doi.org/10.3389/fonc.2021.677605DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8217881PMC
June 2021

Acid-Induced Self-Catalyzing Platform Based on Dextran-Coated Copper Peroxide Nanoaggregates for Biofilm Treatment.

ACS Appl Mater Interfaces 2021 Jun 18;13(25):29269-29280. Epub 2021 Jun 18.

College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China.

Nanoantibacterial agents based on catalytic activity were limited due to the low levels of endogenous HO in the microenvironment of bacterial biofilms. However, the additional HO will trigger more side effects to healthy surroundings, which is still a great challenge. Herein, we report an acid-induced self-catalyzing platform based on dextran-coated copper peroxide nanoaggregates (DCPNAs) for antibiofilm and local infection therapy applications. The dextran-functionalized DCPNAs were mediated and conveniently purified via a dextran and ethanol precipitation method, which can also cluster nanodots into nanoaggregates and show good penetrability as well as biocompatibility. Bacterial biofilms were inhibited and destroyed by the reactive oxygen species generated from the Fenton reaction between the Cu and HO released from DCPNAs in an acidic environment, which did not require additional HO. As expected, the DCPNAs exhibit low cytotoxicity and excellent acid-induced antibacterial and antibiofilm ability. Moreover, the DCPNAs realized great therapeutic outcomes in the application for in vivo wound healing. The overall excellent properties associated with the DCPNAs highlight that they could be considered as a kind of ideal antimicrobial agents for microbial biofilm infection treatment.
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http://dx.doi.org/10.1021/acsami.1c03409DOI Listing
June 2021

A Genomic-Clinicopathologic Nomogram for the Prediction of Lymph Node Invasion in Prostate Cancer.

J Oncol 2021 26;2021:5554708. Epub 2021 May 26.

Department of Urology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China.

Background: Lymph node status is important for treatment decision making in prostate cancer (PCa). We aimed to develop a genomic-clinicopathologic nomogram for the prediction of lymph node invasion (LNI) in PCa.

Methods: Differentially expressed genes between LNI and non-LNI PCa samples were identified in the Cancer Genome Atlas database. Univariate Cox regression analysis and minimum redundancy maximum relevance were performed for gene selection. The synthetic minority oversampling technique (SMOTE) was conducted to balance the minority group (LNI group). Machine learning models were constructed in the training set and assessed in the validation set. Univariable logistic regression and multivariable logistic regression were applied to build a nomogram. Furthermore, the RNA-sequence data from our center were used to validate the expression levels of hub genes between five matched primary PCa and the corresponding LNI samples.

Results: The 37-gene-based support vector machine (SVM) model had the optimal synthesized performance in the SMOTE-balanced training (area under the curve (AUC): 0.947) and validation (AUC: 0.901) sets. Incorporating the SVM-based risk score and the Gleason grade, the genomic-clinicopathologic nomogram demonstrated good prediction and calibration both in the SMOTE-balanced training (AUC: 0.946) and validation (AUC: 0.910) sets. The dysregulated expression of hub genes between PCa and LNI samples was also validated.

Conclusion: The proposed nomogram combining the 37-gene-based SVM model with the Gleason grade had the potential to preoperatively predict LNI in PCa. Some of the hub genes should be prioritized for functional studies and mechanistic analyses.
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http://dx.doi.org/10.1155/2021/5554708DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8172299PMC
May 2021

Visible light responsive, self-activated bionanocomposite films with sustained antimicrobial activity for food packaging.

Food Chem 2021 Nov 25;362:130201. Epub 2021 May 25.

College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China. Electronic address:

The research on a new type of low-cost, less-loss and adjustable sustained antibacterial activity food packaging films with self-activation ability and great industrialization potentiality is of great scientific and technological interest. Herein, a novel chitosan/negatively charged graphitic carbon nitride self-activation bionanocomposite films was prepared by one-step electrostatic self-assembly. First, the antibacterial efficiency of this film could reach to 99.8 ± 0.26% against E. coli and 99.9 ± 0.04% against S. aureus through self-activated under visible light. Second, this film can effectively extend the shelf life of tangerines to 24 days. Hemolysis and cell experiment test proved that this film was safe and nontoxic. Finally, negatively charged graphitic carbon nitride with low-cost can improve the mechanical, thermal and hydrophobic properties of neat chitosan films. This work can provide a new pathway for the preparation of low-cost packaging films with excellent visible light responsive property and sustainable antibacterial activity.
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http://dx.doi.org/10.1016/j.foodchem.2021.130201DOI Listing
November 2021

Circ_HECW2 regulates LPS-induced apoptosis of chondrocytes via miR-93 methylation.

Immun Inflamm Dis 2021 Sep 2;9(3):943-949. Epub 2021 Jun 2.

Department of Sports Medicine, Peking University Shenzhen Hospital, Shenzhen, Guangdong, China.

Introduction: Circ_HECW2 plays a key role in lipopolysaccharide (LPS)-induced signal transduction, which is critical in osteoarthritis (OA). Thus, we analyzed the role of Circ_HECW2 in osteoarthritis.

Methods: The expression of Circ_HECW2 and miR-93 was examined using reverse-transcription polymerase chain reaction. Cell apoptosis was evaluated using Annexin V-FITC Apoptosis Detection Kit.

Results: Circ_HECW2 and miR-93 were inversely correlated, with Circ_HECW2 upregulated and miR-93 downregulated in OA and LPS-induced chondrocytes. Circ_HECW2 overexpression inhibited miR-93 expression and increased methylation of miR-93 coding gene. Cell apoptosis analysis showed that Circ_HECW2 overexpression increased LPS-induced chondrocyte apoptosis, while MiR-93 overexpression reversed the effects of Circ_HECW2 on chondrocyte apoptosis.

Conclusion: In summary, our data revealed that the Circ_HECW2 is highly expressed in OA and might inhibit miR-93 expression through methylation to affect LPS-induced chondrocyte apoptosis.
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http://dx.doi.org/10.1002/iid3.453DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8342212PMC
September 2021

Dynamic switching of coaxial focus based on terahertz meta-lens.

Appl Opt 2021 May;60(13):3629-3633

We propose an active meta-lens that can dynamically switch the coaxial focus on three statuses with the external optical pump. The meta-lens composes of two concentric sets of complementary split-ring resonator (CSRR) arrays, which function at different focal lengths, atop the silicon on sapphire substrate. With specifically structured phase distribution, the meta-lens can form completely separated double foci simultaneously. Through illuminating the internal or external CSRR arrays individually with patterned optical pump, the meta-lens switches to single focus at different points. The proposed design provides a new avenue for developing terahertz multifunctional devices applied in microscope imaging and tomography.
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http://dx.doi.org/10.1364/AO.421906DOI Listing
May 2021

A Bone-Hamstring Autograft for Arthroscopic Anterior Cruciate Ligament Reconstruction.

Arthrosc Tech 2021 Apr 8;10(4):e1033-e1038. Epub 2021 Mar 8.

Department of Sports Medicine and Rehabilitation, Peking University Shenzhen Hospital, Shenzhen, China.

We describe a bone-hamstring autograft for anterior cruciate ligament reconstruction (ACLR). The semitendinosus and gracilis tendons are harvested using an open tendon stripper, keeping these distal tibial insertions intact. The bone-hamstring autograft is harvested using an oscillating saw. This modified autograft has the following advantages: (1) It possesses the potential for healing with the femur owing to its bone plug; (2) it is perfectly suited for various single-bundle reconstruction methods including oval-tunnel and rectangular-tunnel ACLR, as well as other flat ACLR methods; and (3) it is an attractive option for both primary ACLR and revision ACLR owing to its unique characteristics.
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http://dx.doi.org/10.1016/j.eats.2020.12.008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8085407PMC
April 2021

Downregulation of Diacylglycerol kinase zeta (DGKZ) suppresses tumorigenesis and progression of cervical cancer by facilitating cell apoptosis and cell cycle arrest.

Bioengineered 2021 12;12(1):1517-1529

Department of Gynecology and Obstetrics, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi Province, China.

Diacylglycerol kinase zeta (DGKZ) participates in cancer progression. Here, the current work aims to identify the functional role of DGKZ in cervical cancer (CC). DGKZ expression in cervical cancer tissues and paired adjacent normal cervical tissues was assessed using Immunohistochemistry assay. SiHa and HeLa cells were transfected with lentivirus plasmids (sh-DGKZ or sh-NC) to evaluate the effects of DGKZ knockdown on cell proliferation, apoptosis and cell cycle distribution in vitro. Furthermore, BALB/c nude mice were injected subcutaneously with Lentivirus-sh-DGKZ-SiHa cells or Lentivirus-sh-NC-SiHa cells to analyze the influence of DGKZ silencing on tumor growth of CC in vivo. Moreover, the potential molecular mechanisms were predicted by GO and KEGG analysis and preliminarily explored through PathScan Analysis. Elevated DGKZ expression in cervical tumor was observed. Downregulation of DGKZ repressed proliferation and boosted apoptosis of SiHa and HeLa cells and induced cell cycle arrest at G0/G1 phase. In addition, Knockdown of DGKZ restrained tumor growth in tumor xenograft mice. Importantly, GO and KEGG analysis displayed that differentially expressed proteins induced by silence of DGKZ were mostly enriched in autophagy or mitophagy, indicating that the functions of DGKZ on cell proliferation and tumor growth may be associated with autophagy or mitophagy. PathScan analysis presented that PI3K-AKT and TAK1-NF-κB signaling pathways were prominently inhibited in SiHa cells transfected with sh-DGKZ. In summary, downregulation of DGKZ impeded cell proliferation, boosted cell apoptosis and induced cell cycle arrest to suppress tumorigenesis and progression of cervical cancer.
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http://dx.doi.org/10.1080/21655979.2021.1918505DOI Listing
December 2021

Loss of microRNA-147 function alleviates synovial inflammation through ZNF148 in rheumatoid and experimental arthritis.

Eur J Immunol 2021 Aug 5;51(8):2062-2073. Epub 2021 May 5.

Department of Biochemistry and Molecular Biology, Institute of Molecular and Translational Medicine (IMTM), School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi, P. R. China.

MicroRNA-147 (miR-147) had been previously found induced in synoviocytes by inflammatory stimuli derived from T cells in experimental arthritis. This study was designed to verify whether loss of its function might alleviate inflammatory events in joints of experimental and rheumatoid arthritis (RA). Dark Agouti (DA) rats were injected intradermally with pristane to induce arthritis, and rno-miR-147 antagomir was locally administrated into individual ankle compared with negative control or rno-miR-155-5p antagomir (potential positive control). Arthritis onset, macroscopic severity, and pathological changes were monitored. While in vitro, gain or loss function of hsa-miR-147b-3p/hsa-miR-155-5p and ZNF148 was achieved in human synovial fibroblast cell line SW982 and RA synovial fibroblasts (RASF). The expression of miRNAs and mRNAs was detected by using RT-quantitative PCR, and protein expression was detected by using Western blotting. Anti-miR-147 therapy could alleviate the severity, especially for the synovitis and joint destruction in experimental arthritis. Gain of hsa-miR-147b-3p/hsa-miR-155-5p function in TNF-α stimulated SW982 and RASF cells could upregulate, in contrast, loss of hsa-miR-147b-3p/hsa-miR-155-5p function could downregulate the gene expression of TNF-α, IL-6, MMP3, and MMP13. Hence, such alteration could participate in synovial inflammation and joint destruction. RNAi of ZNF148, a miR-147's target, increased gene expression of TNF-α, IL-6, MMP3, and MMP13 in SW982 and RASF cells. Also, mRNA sequencing data showed that hsa-miR-147b-3p mimic and ZNF148 siRNA commonly regulated the gene expression of CCL3 and DEPTOR as well as some arthritis and inflammation-related pathways. Taken together, miR-147b-3p contributes to synovial inflammation through repressing ZNF148 in RA and experimental arthritis.
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http://dx.doi.org/10.1002/eji.202048850DOI Listing
August 2021

Conditional Mapping Identified Quantitative Trait Loci for Grain Protein Concentration Expressing Independently of Grain Yield in Canadian Durum Wheat.

Front Plant Sci 2021 24;12:642955. Epub 2021 Mar 24.

Swift Current Research and Development Centre, Agriculture and Agri-Food Canada, Swift Current, SK, Canada.

Grain protein concentration (GPC) is an important trait in durum cultivar development as a major determinant of the nutritional value of grain and end-use product quality. However, it is challenging to simultaneously select both GPC and grain yield (GY) due to the negative correlation between them. To characterize quantitative trait loci (QTL) for GPC and understand the genetic relationship between GPC and GY in Canadian durum wheat, we performed both traditional and conditional QTL mapping using a doubled haploid (DH) population of 162 lines derived from Pelissier × Strongfield. The population was grown in the field over 5 years and GPC was measured. QTL contributing to GPC were detected on chromosome 1B, 2B, 3A, 5B, 7A, and 7B using traditional mapping. One major QTL on 3A () was consistently detected over 3 years accounting for 9.4-18.1% of the phenotypic variance, with the favorable allele derived from Pelissier. Another major QTL on 7A () detected in 3 years explained 6.9-14.8% of the phenotypic variance, with the beneficial allele derived from Strongfield. Comparison of the QTL described here with the results previously reported led to the identification of one novel major QTL on 3A () and five novel minor QTL on 1B, 2B and 3A. Four QTL were common between traditional and conditional mapping, with and detected in multiple environments. The QTL identified by conditional mapping were independent or partially independent of GY, making them of great importance for development of high GPC and high yielding durum.
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http://dx.doi.org/10.3389/fpls.2021.642955DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8024689PMC
March 2021

PNOC Expressed by B Cells in Cholangiocarcinoma Was Survival Related and LAIR2 Could Be a T Cell Exhaustion Biomarker in Tumor Microenvironment: Characterization of Immune Microenvironment Combining Single-Cell and Bulk Sequencing Technology.

Front Immunol 2021 24;12:647209. Epub 2021 Mar 24.

Liver Cancer Institute, Zhongshan Hospital, Fudan University and Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Shanghai, China.

Background: Cholangiocarcinoma was a highly malignant liver cancer with poor prognosis, and immune infiltration status was considered an important factor in response to immunotherapy. In this investigation, we tried to locate immune infiltration related genes of cholangiocarcinoma through combination of bulk-sequencing and single-cell sequencing technology.

Methods: Single sample gene set enrichment analysis was used to annotate immune infiltration status in datasets of TCGA CHOL, GSE32225, and GSE26566. Differentially expressed genes between high- and low-infiltrated groups in TCGA dataset were yielded and further compressed in other two datasets through backward stepwise regression in R environment. Single-cell sequencing data of GSE138709 was loaded by Seurat software and was used to examined the expression of infiltration-related gene set. Pathway changes in malignant cell populations were analyzed through scTPA web tool.

Results: There were 43 genes differentially expressed between high- and low-immune infiltrated patients, and after further compression, PNOC and LAIR2 were significantly correlated with high immune infiltration status in cholangiocarcinoma. Through analysis of single-cell sequencing data, PNOC was mainly expressed by infiltrated B cells in tumor microenvironment, while LAIR2 was expressed by Treg cells and partial GZMB+ CD8 T cells, which were survival related and increased in tumor tissues. High B cell infiltration levels were related to better overall survival. Also, malignant cell populations demonstrated functionally different roles in tumor progression.

Conclusion: PNOC and LAIR2 were biomarkers for immune infiltration evaluation in cholangiocarcinoma. PNOC, expressed by B cells, could predict better survival of patients, while LAIR2 was a potential marker for exhaustive T cell populations, correlating with worse survival of patients.
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http://dx.doi.org/10.3389/fimmu.2021.647209DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8024580PMC
March 2021

Differentiation of Glioma Mimicking Encephalitis and Encephalitis Using Multiparametric MR-Based Deep Learning.

Front Oncol 2021 15;11:639062. Epub 2021 Mar 15.

Department of Radiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Background: Computational aid for diagnosis based on convolutional neural network (CNN) is promising to improve clinical diagnostic performance. Therefore, we applied pretrained CNN models in multiparametric magnetic resonance (MR) images to classify glioma mimicking encephalitis and encephalitis.

Methods: A data set containing 3064 MRI brain images from 164 patients with a final diagnosis of glioma (n = 56) and encephalitis (n = 108) patients and divided into training and testing sets. We applied three MRI modalities [fluid attenuated inversion recovery (FLAIR), contrast enhanced-T1 weighted imaging (CE-T1WI) and T2 weighted imaging (T2WI)] as the input data to build three pretrained deep CNN models (Alexnet, ResNet-50, and Inception-v3), and then compared their classification performance with radiologists' diagnostic performance. These models were evaluated by using the area under the receiver operator characteristic curve (AUC) of a five-fold cross-validation and the accuracy, sensitivity, specificity were analyzed.

Results: The three pretrained CNN models all had AUC values over 0.9 with excellent performance. The highest classification accuracy of 97.57% was achieved by the Inception-v3 model based on the T2WI data. In addition, Inception-v3 performed statistically significantly better than the Alexnet architecture (<0.05). For Inception-v3 and ResNet-50 models, T2WI offered the highest accuracy, followed by CE-T1WI and FLAIR. The performance of Inception-v3 and ResNet-50 had a significant difference with radiologists (<0.05), but there was no significant difference between the results of the Alexnet and those of a more experienced radiologist (0.05).

Conclusions: The pretrained CNN models can automatically and accurately classify these two diseases and further help to improving clinical diagnostic performance.
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http://dx.doi.org/10.3389/fonc.2021.639062DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8005708PMC
March 2021

Synovial chondromatosis of the temporomandibular joint with 400 loose bodies: a case report and literature review.

J Int Med Res 2021 Mar;49(3):3000605211000526

Department of Stomatology, Zhejiang Provincial People's Hospital (People's Hospital of Hangzhou Medical College), Hangzhou, Zhejiang, China.

Synovial chondromatosis (SC) is a benign condition characterized by the formation of metaplastic cartilage in the synovial membrane of the joint, resulting in numerous attached and unattached osteocartilaginous bodies. SC mostly affects the large synovial joints, especially the knee, hip, elbow, and ankle, whereas involvement of the temporomandibular joint (TMJ) is rare. Approximately 240 cases of SC of the TMJ have been reported in the English-language literature to date. The number of loose bodies varies among patients but usually ranges from the dozens to around 100. We herein report a case of SC of the TMJ accompanied by approximately 400 loose bodies in a healthy 53-year-old woman. Such a high number of loose bodies within a small space is extremely rare. We also include a brief discussion about the differential diagnoses and current diagnostic approaches to SC of the TMJ. Notably, delayed diagnosis or misdiagnosis is common because of the nonspecific nature of the presenting complaints.
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http://dx.doi.org/10.1177/03000605211000526DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7995452PMC
March 2021

Probing lattice vibration of alkali halide crystals by broadband terahertz spectroscopy.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Jun 8;254:119671. Epub 2021 Mar 8.

Center for Terahertz Waves and College of Precision Instrument and Optoelectronics Engineering, Tianjin University, Tianjin 300072, China. Electronic address:

Terahertz spectral features of alkali halide crystals were studied with the combination of broadband terahertz time-domain spectroscopy and the solid-state-based density functional theory calculations. To understand the particular modes of the observed terahertz features of the alkali halide crystals, the resonant modes of KCl and CsCl were analyzed using face-centered cubic and body-centered cubic lattice models, respectively. The results show that the characteristic terahertz absorption peaks could be assigned to the lattice vibration of the ionic crystals. Furthermore, the terahertz responses of a series of alkali halides were recorded, and obvious absorption peaks were observed in each salt in the frequency region below 8.5 THz. What is more interestingly is that the frequencies of these observed peaks are red-shifted with the increases of the mass and radius of the ions. This correlation between the resonant frequency of the lattice vibration, the reduced atomic mass, and the equilibrium distance between the ions agrees well with the harmonic oscillator model.
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http://dx.doi.org/10.1016/j.saa.2021.119671DOI Listing
June 2021

A biomechanical analysis of four anterior cervical techniques to treating multilevel cervical spondylotic myelopathy: a finite element study.

BMC Musculoskelet Disord 2021 Mar 15;22(1):278. Epub 2021 Mar 15.

Department of Orthopaedics, First Affiliated Hospital of Dalian Medical University, Dalian, People's Republic of China.

Background: The decision to treat multilevel cervical spondylotic myelopathy (MCSM) remains controversial. The purpose of this study is to compare the biomechanical characteristics of the intervertebral discs at the adjacent segments and internal fixation, and to provide scientific experimental evidence for surgical treatment of MCSM.

Methods: An intact C2-C7 cervical spine model was developed and validated. Four additional models were developed from the fusion model, including multilevel anterior cervical discectomy and fusion (mACDF), anterior cervical corpectomy and fusion (ACCF), hybrid decompression and fusion (HDF), and mACDF with cage alone (mACDF-CA). Biomechanical characteristics on the plate and the disc of adjacent levels (C2/3, C6/7) were comparatively analyzed.

Results: Of the four models, stress on the upper (C2/3) adjacent intervertebral disc was the lowest in the mACDF-CA group and highest in the ACCF group. Stress on the intervertebral discs at adjacent segments was higher for the upper C2/3 than the lower C6/7 intervertebral disc. In all models, the mACDF-CA group had the lowest stress on the intervertebral disc, while the ACCF group had the highest stress. In the three surgical models with titanium plate fixation (mACDF, ACCF, and HDF), the ACCF group had the highest stress at the titanium plate-screw interface, while the mACDF group had the lowest stress.

Conclusion: Among the four anterior cervical reconstructive techniques for MCSM, mACDF-CA makes little effect on the adjacent disc stress, which might reduce the incidence of adjacent segment degeneration (ASD) after fusion. However, the accompanying risk of the increased incidence of cage subsidence should never be neglected.
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http://dx.doi.org/10.1186/s12891-021-04150-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7962321PMC
March 2021
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