Publications by authors named "Zhang Wang"

338 Publications

A comprehensive synthesis unveils the mysteries of phosphate-solubilizing microbes.

Biol Rev Camb Philos Soc 2021 Jul 21. Epub 2021 Jul 21.

Institute of Ecological Science, Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou, 510631, PR China.

Phosphate-solubilizing microbes (PSMs) drive the biogeochemical cycling of phosphorus (P) and hold promise for sustainable agriculture. However, their global distribution, overall diversity and application potential remain unknown. Here, we present the first synthesis of their biogeography, diversity and utility, employing data from 399 papers published between 1981 and 2017, the results of a nationwide field survey in China consisting of 367 soil samples, and a genetic analysis of 12986 genome-sequenced prokaryotic strains. We show that at continental to global scales, the population density of PSMs in environmental samples is correlated with total P rather than pH. Remarkably, positive relationships exist between the population density of soil PSMs and available P, nitrate-nitrogen and dissolved organic carbon in soil, reflecting functional couplings between PSMs and microbes driving biogeochemical cycles of nitrogen and carbon. More than 2704 strains affiliated with at least nine archaeal, 88 fungal and 336 bacterial species were reported as PSMs. Only 2.59% of these strains have been tested for their efficiencies in improving crop growth or yield under field conditions, providing evidence that PSMs are more likely to exert positive effects on wheat growing in alkaline P-deficient soils. Our systematic genetic analysis reveals five promising PSM genera deserving much more attention.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/brv.12779DOI Listing
July 2021

Significantly high expression of NUP37 leads to poor prognosis of glioma patients by promoting the proliferation of glioma cells.

Cancer Med 2021 Jul 15. Epub 2021 Jul 15.

Department of Orthopaedic, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, School of Clinical Medicine, Henan University, Zhengzhou, China.

Background: The carcinogenic effect of NUP37 has been reported recently in a variety of tumors, but its research in the field of glioma has not been paid attention. The main purpose of this study is to reveal the relationship between NUP37 and prognosis or clinical characteristics of glioma patients.

Methods: First, as a retrospective study, this study included thousands of tissue samples based on a variety of public databases and clinicopathological tissues. Second, a series of bioinformatics analysis methods were used to analyze the NUP37 and glioma samples from multiple databases such as the CGGA, TCGA, GEO, HPA, and GEPIA. Third, to analyze the relationship between the expression level of NUP37 in tumor tissues and cells and a variety of clinical prognostic molecular characteristics, whether it can be an independent risk factor leading to poor prognosis in glioma and whether it has clinical diagnostic value; GSEA was used to analyze the cancer-related signaling pathways that may be activated by high expression of NUP37. Fifth, CMap was used to analyze small molecule drugs that may inhibit NUP37 expression. Finally, the meta-analysis of thousands of tissue samples from seven datasets and cell proliferation and migration experiments confirmed that NUP37 has a malignant effect on glioma.

Results: NUP37 is highly expressed in glioma patient tissues and glioma cells, significantly correlates with reduced overall survival, and may serve as an independent prognostic factor with some diagnostic value. Silencing NUP37 suppresses malignant biological behaviors of glioma cells. 4 small molecule drugs that had potential targeting inhibitory effects on NUP37 overexpression.

Conclusions: This study demonstrates for the first time a malignant role of NUP37 in glioma and provides a vision to unravel the complex pathological mechanisms of glioma and a potentially valuable biomarker for implementing individualized diagnosis and treatment of glioma.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/cam4.3954DOI Listing
July 2021

Rational Design of Embedded CoTe Nanoparticles in Freestanding N-Doped Multichannel Carbon Fibers for Sodium-Ion Batteries with Ultralong Cycle Lifespan.

ACS Appl Mater Interfaces 2021 Jul 14. Epub 2021 Jul 14.

School of Materials and Energy, Guangzhou Key Laboratory of Low-Dimensional Materials and Energy Storage Devices, Guangdong University of Technology, Guangzhou 510006, P. R. China.

Although sodium-ion batteries (SIBs) have high potential for applications in large-scale energy storage, their limited cycle life and unsatisfactory energy density hinder their commercial applications. Here, a superior stable CoTe/carbon anode, in which CoTe nanoparticles are embedded in freestanding N-doped multichannel carbon fiber ([email protected]), with ultralong cycle life for SIBs, is reported. Specifically, CoTe nanoparticles are uniformly dispersed in the carbon matrix to inhibit its volume expansion and agglomeration during the desodiation/sodiation process, enabling a high-capacity and stable energy storage (retains 204.3 mAh g/612.9 mAh cm at 1 A g after 2000 cycles with an ultralow capacity decay of 0.016% per cycle). Moreover, a [email protected] electrode exhibits a pseudocapacitive-dominated behavior, enabling the high-rate performance (152.4 mAh g/457.2 mAh cm at 10 A g). The battery-capacitive dual-model reaction mechanism and outstanding reversibility of the [email protected] composite are systematically investigated by XRD/SEM/TEM and a galvanostatic intermittent titration technique test, as well as surface capacitance calculations. More importantly, the fabricated sodium-ion [email protected]//P2-NaNMMT-4 full cell delivers a stable reversible capacity of 445 Wh kg at 0.2 A g and an excellent rate performance. The facile synthetic approach together with unique nanostructural design, provides a meaningful reference for the rational design of next-generation ultralong cycle-life SIBs anodes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.1c06794DOI Listing
July 2021

Spontaneous fundus lesions in elderly monkeys: An ideal model for age-related macular degeneration and high myopia clinical research.

Life Sci 2021 Jul 10;282:119811. Epub 2021 Jul 10.

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China. Electronic address:

Aims: Age-related macular degeneration (AMD) and high myopia are frequent causes of progressive visual impairment, so it is critical to identify animal models with resembling human retinal physiology, AMD and high myopia pathological features for therapeutic studies.

Main Methods: We screened elderly cynomolgus monkeys for fundus lesions by slit-lamp biomicroscope combined with fundus pre-set lens and further examined positive cases by color fundus photography (CFP), optical coherence tomography (OCT), fundus fluorescein angiography (FFA), streak retinoscopy, and A-scan ultrasonography.

Key Findings: Among the 156 animals examined, 10 males and 5 females (30 eyes) exhibited fundus abnormalities (9.6% prevalence). Multi-modal imaging revealed drusen in 20 eyes of 11 animals (prevalence rate of 7.1%), tessellated fundus in 22 eyes of 11 animals, and myopia choroidal neovascularization (CNV) in 4 eyes of 3 animals.

Significance: Aged cynomolgus monkeys exhibit spontaneous fundus lesions resembling human AMD and high myopia, which could be an ideal model for clinical research.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.lfs.2021.119811DOI Listing
July 2021

Construction of a Bioinspired Hierarchical BiVO/BiOCl Heterojunction and Its Enhanced Photocatalytic Activity for Phenol Degradation.

ACS Appl Mater Interfaces 2021 Jul 4;13(28):32906-32915. Epub 2021 Jul 4.

School of Chemical Engineering, University of New South Wales, Sydney, New South Wales 2052, Australia.

Development of a p-n heterojunction to achieve efficient degradation of organic pollutants is a promising approach in the field of photocatalysis. Herein, BiVO with bioinspired hierarchical structures was prepared with the sol-gel method and combined with BiOCl nanoplates to construct a 3D/2D configuration an deposition route. The hierarchical BiVO served as an excellent substrate to achieve the uniform loading of BiOCl nanoplates. The obtained 3D/2D BiVO/BiOCl hybrids exhibited significantly enhanced photocatalytic efficiency for degrading phenol under visible light irradiation, with a first-order reaction rate constant that was 9.9 and 1.9 times higher than those of hierarchical BiVO and the BiVO/BiOCl hybrids without hierarchical structures, respectively. Moreover, the hierarchical BiVO/BiOCl also displayed good photochemical stability for the degradation of phenol after three recycles. The p-n heterojunction and hierarchical structure worked together to form a spatial conductive network framework, which possessed improved visible light absorption, high specific surface area, as well as effective separation and transfer of photogenerated charge carriers.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.1c05117DOI Listing
July 2021

The R2R3-type MYB transcription factor MdMYB90-like is responsible for the enhanced skin color of an apple bud sport mutant.

Hortic Res 2021 Jul 1;8(1):156. Epub 2021 Jul 1.

College of Horticulture, Nanjing Agricultural University, No. 1 Weigang, 8210095, Nanjing, China.

The anthocyanin content in apple skin determines its red coloration, as seen in a Fuji apple mutant. Comparative RNA-seq analysis was performed to determine differentially expressed genes at different fruit development stages between the wild-type and the skin color mutant. A novel R2R3-MYB transcription factor, MdMYB90-like, was uncovered as the key regulatory gene for enhanced coloration in the mutant. The expression of MdMYB90-like was 21.3 times higher in the mutant. MdMYB90-like regulates anthocyanin biosynthesis directly through the activation of anthocyanin biosynthesis genes and indirectly through the activation of other transcription factors that activate anthocyanin biosynthesis. MdMYB90-like bound to the promoters of both structural genes (MdCHS and MdUFGT) and other transcription factor genes (MdMYB1 and MdbHLH3) in the yeast one-hybrid system, electrophoretic mobility shift assay, and dual-luciferase assay. Transgenic analysis showed that MdMYB90-like was localized in the nucleus, and its overexpression induced the expression of other anthocyanin-related genes, including MdCHS, MdCHI, MdANS, MdUFGT, MdbHLH3, and MdMYB1. The mutant had reduced levels of DNA methylation in two regions (-1183 to -988 and -2018 to -1778) of the MdMYB90-like gene promoter, which might explain the enhanced expression of the gene and the increased anthocyanin content in the mutant apple skin.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41438-021-00590-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8245648PMC
July 2021

Methyl-CpG-Binding Domain Protein 3 Promotes Seizures by Recruiting Methyltransferase DNMT1 to Enhance TREM2 Methylation.

Neurochem Res 2021 Sep 25;46(9):2451-2462. Epub 2021 Jun 25.

Department of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, No. 23, Youzheng Street, Nangang District, Harbin, 150001, Heilongjiang Province, People's Republic of China.

Epilepsy represents a hazardous neurological disorder, underpinned by a pathophysiological process that is yet to be fully understood. Here, we aimed to elucidate the effect of methyl-CpG-binding domain protein 3 (MBD3) on hippocampal neuronal damage in epileptic mice by targeting the phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) pathway. The expression of MBD3 was determined by Western blot in a hippocampal neuronal culture (HNC) epileptic model established using the low Mg+ECF culture method. The interaction between MBD3 and DNA methyltransferase 1 (DNMT1) was determined via co-immunoprecipitation and mass spectrometry analysis. Bisulfite modification and sequencing was performed to evaluate the degree of methylation of triggering receptor expressed on myeloid cells 2 (TREM2). The viability and apoptosis of hippocampal neurons were detected by CCK-8 and TUNEL assays, respectively. Finally, the effect of MBD3 was verified in vivo. MBD3 was highly expressed in the HNC model of epilepsy, with its interaction with DNMT1 found to promote the hypermethylation of TREM2 at site cg25748868. Additionally, decreased TREM2 and inhibited PI3K/Akt pathway was observed in the HNC epileptic model. Simultaneous inhibition of MBD3 and DNMT1 decreased the methylation level at cg25748868, up-regulated TREM2 expression, and activated the PI3K/Akt pathway, thereby arresting neuronal damage. Inhibition of MBD3 reduced the level of epileptic seizures, down-regulated cg25748868 methylation, activated TREM2-mediated signaling pathways, and alleviated hippocampal neuronal damage in the acute seizure mouse models. The present study unveiled that MBD3 and DNMT1 synergistically enhanced hypermethylation of cg25748868 in TREM2, and promoted the onset of epilepsy via inhibition of the PI3K/Akt pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11064-021-03371-5DOI Listing
September 2021

High-resolution light field prints by nanoscale 3D printing.

Nat Commun 2021 Jun 17;12(1):3728. Epub 2021 Jun 17.

Engineering Product Development, Singapore University of Technology and Design, Singapore, Singapore.

A light field print (LFP) displays three-dimensional (3D) information to the naked-eye observer under ambient white light illumination. Changing perspectives of a 3D image are seen by the observer from varying angles. However, LFPs appear pixelated due to limited resolution and misalignment between their lenses and colour pixels. A promising solution to create high-resolution LFPs is through the use of advanced nanofabrication techniques. Here, we use two-photon polymerization lithography as a one-step nanoscale 3D printer to directly fabricate LFPs out of transparent resin. This approach produces simultaneously high spatial resolution (29-45 µm) and high angular resolution (~1.6°) images with smooth motion parallax across 15 × 15 views. Notably, the smallest colour pixel consists of only a single nanopillar (~300 nm diameter). Our LFP signifies a step towards hyper-realistic 3D images that can be applied in print media and security tags for high-value goods.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-021-23964-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8211842PMC
June 2021

Synergy of interference, scattering and pigmentation for structural coloration of moth.

Soft Matter 2021 Jul;17(26):6477-6485

Institute of Physics Belgrade, Pregrevica 18, 11080 Zemun, Belgrade, Serbia.

Structural and pigment colorations are omnipresent in insects, producing a range of colors for camouflage, warning, mimicry and other strategies necessary for survival. Structural coloration has attracted a lot of attention due to its significance in biophotonics, biomimetics and even esthetic appeal. The coupling of structural and pigment colorations has been largely unnoticed. Herein we show how pigments, scattering and interference work together in two-dimensional waveguiding structures to produce the coloration of Jordanita globulariae (Huebner, 1793), a moth whose forewings sparkle with slightly iridescent green scales. We show that subwavelength structures scatter and couple light into a concave multilayered structure to enhance the absorption of pigments. A finite element method (FEM) model, adequately describing the photonic properties of J. globulariae, was developed based on the nanoscale architecture of the insect's wing scales. The principle of absorption enhanced by scattering and waveguiding is present in many insect species and might be imitated to tailor the spectral properties of optical devices.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1sm00157dDOI Listing
July 2021

Bioinspired Soft Robotic Fingers with Sequential Motion Based on Tendon-Driven Mechanisms.

Soft Robot 2021 Jun 11. Epub 2021 Jun 11.

School of Aeronautics and Astronautics, Sichuan University, Chengdu, China.

The conformability is yet a challenge for most soft robotic grippers due to the continuous motion and deformation of these machines under external force. Herein, inspired by the movement mechanism of human fingers, we propose a novel tendon-driven soft robotic finger with a preprogrammed bending configuration and a human finger like sequential motion that can be obtained by matching the stiffness gradient of the finger joints with three-dimensional (3D) printing technology. The contents of this article are organized as follows. First, the effect of the anisotropy caused by 3D printing filling direction on the mechanical property is investigated by tensile test. Then, kinematic, stiffness, and fingertip trajectory models are established to analyze the influence of the cross-section thickness and width on the bending and bearing capacity of the finger joint. Furthermore, several experiments are conducted on a self-built experimental platform to evaluate the advantages of sequential motion induced by stiffness gradients. Results reveal that soft robotic fingers with sequential motion show excellent conformability on the object surfaces with various curvatures and outperform nonsequential motion fingers with larger envelop range. Without changing motion trajectories of the fingertip, the deformability of the finger can be tuned by adjusting only the stiffness of the joint. Besides, a two-finger gripper is developed, which presents the capability of grasping objects with different shapes and weights in practical applications. The sequential motion mechanism proposed in this study shows promising potential in soft grippers and robotic design.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1089/soro.2021.0009DOI Listing
June 2021

Traditional Indian medicine in China: The status quo of recognition, development and research.

J Ethnopharmacol 2021 Jun 8;279:114317. Epub 2021 Jun 8.

College of Ethnomedicine, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China; Research Institute of Traditional Indian Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China. Electronic address:

Ethnopharmacological Relevance: Traditional Indian medicine is one of the oldest medical systems and remains popular worldwide. Traditional medicine systems in China and India have historical origins pertaining to mutual learning, reference, and development from medical theory to the drugs used. The exchange of traditional medicine between China and India began in the Qin and Han Dynasties (221 BC-220 AD), prospered in the Tang Dynasty (618-907 AD), and declined after the Song Dynasty (960-1279 AD). It was also directly related to the rise and fall of Buddhism. The traditional medicines of the two countries are highly complementary because of differences in geographical climate and the modernisation process of traditional medicine.

Aim: This review aimed to understand the spread and development of traditional Indian medicine in China to further promote exchange and cooperation between China and India in the field of traditional medicine.

Materials And Methods: We performed a systematic search of MEDLINE via PubMed, CNKI, Science Direct, Sci-Hub, and other databases using the terms 'traditional Indian medicine' or 'Indian medicine' or 'Ayurveda' or 'Yoga' or 'Unani', and limiting the search to articles published between 1958 and 2019. We analysed the sources, publication date, type, and topic of the retrieved articles/studies.

Results: Based on the results of research on traditional Indian medicine carried out by Chinese scholars, 518 academic papers and 60 classic works published in China and abroad were collected. The results showed the following. First, Chinese scholars have systematically investigated traditional Indian medicine including its composition, management, and education; the scale of medicinal and pharmaceutical plants; protective measures of intellectual property rights of traditional medicine; and international promotion of Yoga. Second, studies have examined the development status of traditional Indian medicine in China including the spread of Yoga in the country and the industrial scale of, education in, existing problems in, and clinical research on Yoga. In addition, Chinese scholars conducted research on and the translation of classic works and terms of Ayurveda, and studied the theory, treatment, and medicine thereof. Third, the historical exchange and trading status of traditional medicine between India and China have been discussed, including the exchange of traditional medicine between the two countries, effect of traditional Indian medicine on Chinese Buddhism, and minority medicine and trade in medicinal materials between the two countries.

Conclusion: India attaches great importance to the management of, education in, and industry of traditional medicine, and has made various efforts to protect intellectual property rights. Indian Yoga is very popular in China, and Chinese scholars have conducted some clinical research thereon. However, regulatory systems and legislation for Yoga are lacking in China. At present, traditional Chinese medicine scholars have an enhanced understanding of the term Yoga and less knowledge of the terms Ayurveda, Siddha, Unani, and similar concepts. We suggest that Chinese scholars further study the classic works, basic theories, treatment of clinical diseases, medicinal materials, and prescriptions compounding traditional Indian medicine. The results of this study highlight directions for Chinese scholars to pursue in further studying traditional Indian medicine comprehensively, and will help promote exchange and cooperation between China and India in the field of traditional medicine.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jep.2021.114317DOI Listing
June 2021

Global investigation of estrogen-responsive genes regulating lipid metabolism in the liver of laying hens.

BMC Genomics 2021 Jun 9;22(1):428. Epub 2021 Jun 9.

College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, China.

Background: Estrogen plays an essential role in female development and reproductive function. In chickens, estrogen is critical for lipid metabolism in the liver. The regulatory molecular network of estrogen in chicken liver is poorly understood. To identify estrogen-responsive genes and estrogen functional sites on a genome-wide scale, we determined expression profiles of mRNAs, lncRNAs, and miRNAs in estrogen-treated ((17β-estradiol)) and control chicken livers using RNA-Sequencing (RNA-Seq) and studied the estrogen receptor α binding sites by ChIP-Sequencing (ChIP-Seq).

Results: We identified a total of 990 estrogen-responsive genes, including 962 protein-coding genes, 11 miRNAs, and 17 lncRNAs. Functional enrichment analyses showed that the estrogen-responsive genes were highly enriched in lipid metabolism and biological processes. Integrated analysis of the data of RNA-Seq and ChIP-Seq, identified 191 genes directly targeted by estrogen, including 185 protein-coding genes, 4 miRNAs, and 2 lncRNAs. In vivo and in vitro experiments showed that estrogen decreased the mRNA expression of PPARGC1B, which had been reported to be linked with lipid metabolism, by directly increasing the expression of miR-144-3p.

Conclusions: These results increase our understanding of the functional network of estrogen in chicken liver and also reveal aspects of the molecular mechanism of estrogen-related lipid metabolism.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12864-021-07679-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8190866PMC
June 2021

Comparative analysis of hypothalamus transcriptome between laying hens with different egg-laying rates.

Poult Sci 2021 Jul 11;100(7):101110. Epub 2021 Mar 11.

College of Animal Science, Henan Agricultural University, Zhengzhou 450046, China; Henan Innovative Engineering Research Center of Poultry Germplasm Resource, Henan Agricultural University, Zhengzhou 450046, China; International Joint Research Laboratory for Poultry Breeding of Henan, Henan Agricultural University, Zhengzhou 450046, China. Electronic address:

Egg-laying performance is one of the most important economic traits in the poultry industry. Commercial layers can lay one egg almost every day during their peak-laying period. However, many Chinese indigenous chicken breeds show a relatively low egg-laying rate, even during their peak-laying period. To understand what makes the difference in egg production, we compared the hypothalamus transcriptome profiles of Lushi blue-shelled-egg chickens (LBS), a Chinese indigenous breed with low egg-laying rate and Rhode Island Red chickens (RIR), a commercial layer with relatively high egg-laying rate using RNA-seq. A total of 753 differentially expressed genes (DEGs) were obtained. Of these DEGs, 38 genes were enriched in 2 Gene Ontology (GO) terms, namely reproduction term and the reproductive process term, and 6 KEGG pathways, namely Wnt signaling pathway, Oocyte meiosis, GnRH signaling pathway, Thyroid hormone signaling pathway, Thyroid hormone synthesis and MAPK signaling pathway, which have been long known to be involved in egg production regulation. To further determine the core genes from the 38 DEGs, protein-protein interaction (PPI) network, co-expression network and transcriptional regulatory network analyses were carried out. After integrated analysis and experimental validation, 4 core genes including RAC1, MRE11A, MAP7 and SOX5 were identified as the potential core genes that are responsible for the laying-rate difference between the 2 breeds. These findings paved the way for future investigating the mechanism of egg-laying regulation and enriched the chicken reproductive regulation theory.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.psj.2021.101110DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187251PMC
July 2021

GNG5 is a novel oncogene associated with cell migration, proliferation, and poor prognosis in glioma.

Cancer Cell Int 2021 Jun 7;21(1):297. Epub 2021 Jun 7.

Department of Neurosurgery, The First Affiliate Hospital of Harbin Medical University, 23 Youzheng Street, Nangang District, Harbin, 150001, China.

Background: Although many biomarkers have been reported for detecting glioma, the prognosis for the disease remains poor, and therefore, new biomarkers need to be identified. GNG5, which is part of the G-protein family, has been associated with different malignant tumors, though the role of GNG5 in glioma has not been studied. Therefore, we aimed to identify the relationship between GNG5 and glioma prognosis and identify a new biomarker for the diagnosis and treatment of gliomas.

Methods: We used data on more than a thousand gliomas from multiple databases and clinical data to determine the expression of GNG5 in glioma. Based on clinical data and CGGA database, we identified the correlation between GNG5 and multiple molecular and clinical features and prognosis using various analytical methods. Co-expression analysis and GSEA were performed to detect GNG5-related genes in glioma and possible signaling pathways involved. ESTIMATE, ssGSEA, and TIMER were used to detect the relationship between GNG5 and the immune microenvironment. Functional experiments were performed to explore the function of GNG5 in glioma cells.

Results: GNG5 is highly expressed in gliomas, and its expression level is positively correlated with pathological grade, histological type, age, and tumor recurrence and negatively correlated with isocitrate dehydrogenase mutation, 1p/19 co-deletion, and chemotherapy. Moreover, GNG5 as an independent risk factor was negatively correlated with the overall survival time. GSEA revealed the potential signaling pathways involved in GNG5 function in gliomas, including cell adhesion molecules signaling pathway. The ssGSEA, ESTIMATE, and TIMER based analysis indicated a correlation between GNG5 expression and various immune cells in glioma. In vivo and in vitro experiments showed that GNG5 could participate in glioma cell proliferation and migration.

Conclusions: Based on the large data platform and the use of different databases to corroborate results obtained using various datasets, as well as in vitro and in vivo experiments, our study reveals for the first time that GNG5, as an oncogene, is overexpressed in gliomas and can inhibit the proliferation and migration of glioma cells and lead to poor prognosis of patients. Thus, GNG5 is a potential novel biomarker for the clinical diagnosis and treatment of gliomas.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12935-021-01935-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8186147PMC
June 2021

Long-term pretreatment with alendronate inhibits calvarial defect healing in an osteoporotic rat model.

J Bone Miner Metab 2021 Jun 6. Epub 2021 Jun 6.

Department of Orthopedics, Peking University Third Hospital, No. 49, Huayuan North Road, Haidian District, Beijing, 100191, China.

Introduction: This study aimed to observe the effects of long-term alendronate pretreatment on the healing of osteoporotic calvarial defects, and further investigate the effect of alendronate combined with once-weekly parathyroid hormone following 12 weeks of alendronate treatment in ovariectomized rats.

Materials And Methods: Thirty 3-month-old female rats were ovariectomized, and 24 rats received alendronate for 12 weeks. Then, a critical defect was created in the calvaria of all animals. Immediately after osteotomy, the animals received one of five treatments for 8 weeks: (1) continuation of vehicle (group E), (2) alendronate followed by vehicle (group A), (3) continuation of alendronate (group B), (4) alendronate followed by once-weekly parathyroid hormone alone (group C), or (5) continuation of alendronate combined with once-weekly parathyroid hormone (group D). Calvarial defect healing was assessed using dual-energy X-ray absorptiometry, micro-computed tomography, histology, and sequential fluorescence labeling.

Results: Group E showed a significantly higher volume of newly formed bone than groups A, B, C, and D. Evidence of new dense bone formation in group E was observed histologically. In addition, the immunohistochemical expression of runt-related transcription factor 2 was increased in group E but inhibited in groups A, B, C, and D. Sequential immunofluorescence also showed inhibited mineral apposition in groups A, B, C, and D compared with group E.

Conclusion: The present study shows that long-term pretreatment with alendronate inhibited calvarial defect healing in osteoporotic rats, and this effect could not be reversed by stopping alendronate, switching to parathyroid hormone, or combining with once-weekly parathyroid hormone.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00774-021-01235-0DOI Listing
June 2021

Overexpressed XRCC2 as an independent risk factor for poor prognosis in glioma patients.

Mol Med 2021 May 29;27(1):52. Epub 2021 May 29.

Department of Orthopaedics, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, School of Clinical Medicine, Henan University, Zhengzhou, Henan, China.

Background: XRCC2, a homologous recombination-related gene, has been reported to be associated with a variety of cancers. However, its role in glioma has not been reported. This study aimed to find out the role of XRCC2 in glioma and reveal in which glioma-specific biological processes is XRCC2 involved based on thousands of glioma samples, thereby, providing a new perspective in the treatment and prognostic evaluation of glioma.

Methods: The expression characteristics of XRCC2 in thousands of glioma samples from CGGA and TCGA databases were comprehensively analyzed. Wilcox or Kruskal test was used to analyze the expression pattern of XRCC2 in gliomas with different clinical and molecular features. The effect of XRCC2 on the prognosis of glioma patients was explored by Kaplan-Meier and Cox regression. Gene set enrichment analysis (GSEA) revealed the possible cellular mechanisms involved in XRCC2 in glioma. Connectivity map (CMap) was used to screen small molecule drugs targeting XRCC2 and the expression levels of XRCC2 were verified in glioma cells and tissues by RT-qPCR and immunohistochemical staining.

Results: We found the overexpression of XRCC2 in glioma. Moreover, the overexpressed XRCC2 was associated with a variety of clinical features related to prognosis. Cox and meta-analyses showed that XRCC2 is an independent risk factor for the poor prognosis of glioma. Furthermore, the results of GSEA indicated that overexpressed XRCC2 could promote malignant progression through involved signaling pathways, such as in the cell cycle. Finally, doxazosin, quinostatin, canavanine, and chrysin were identified to exert anti-glioma effects by targeting XRCC2.

Conclusions: This study analyzed the expression pattern of XRCC2 in gliomas and its relationship with prognosis using multiple datasets. This is the first study to show that XRCC2, a novel oncogene, is significantly overexpressed in glioma and can lead to poor prognosis in glioma patients. XRCC2 could serve as a new biomarker for glioma diagnosis, treatment, and prognosis evaluation, thus bringing new insight into the management of glioma.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s10020-021-00316-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164800PMC
May 2021

Full Color and Grayscale Painting with 3D Printed Low-Index Nanopillars.

Nano Lett 2021 Jun 21;21(11):4721-4729. Epub 2021 May 21.

Engineering Product Development Pillar, Singapore University of Technology and Design, 8 Somapah Road, Singapore 487372, Singapore.

Sculpting nanostructures into different geometries in either one or two dimensions produces a wide range of colorful elements in microscopic prints. However, achieving different shades of gray and control of color saturation remain challenging. Here, we report a complete approach to color and grayscale generation based on the tuning of a single nanostructure geometry. Through two-photon polymerization lithography, we systematically investigated color generation from the basic single nanopillar geometry in low-refractive-index ( < 1.6) material. Grayscale and full color palettes were achieved that allow decomposition onto hue, saturation, and brightness values. This approach enabled the "painting" of arbitrary colorful and grayscale images by mapping desired prints to precisely controllable parameters during 3D printing. We further extend our understanding of the scattering properties of the low-refractive-index nanopillar to demonstrate grayscale inversion and color desaturation and steganography at the level of single nanopillars.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.nanolett.1c00979DOI Listing
June 2021

Thermal radiation management by natural photonic structures: Morimus asper funereus case.

J Therm Biol 2021 May 3;98:102932. Epub 2021 Apr 3.

Institute of Physics, University of Belgrade, Pregrevica 118, 11080, Belgrade, Zemun, Serbia.

Convective, conductive and radiative mechanisms of thermal management are extremely important for life. Photonic structures, used to detect infrared radiation (IR) and enhance radiative energy exchange, were observed in a number of organisms. Here we report on sophisticated radiative mechanisms used by Morimus asper funereus, a longicorn beetle whose elytra possess a suitably aligned array of lenslets and blackbodies. Additionally, a dense array of microtrichia hyperuniformly covers blackbodies and operates as a stochastic, full-bandgap, IR-photonic structure. All these features, whose characteristic dimensions cover a range from several hundred down to a few micrometres, operate synergistically to improve the absorption, emission and, possibly, detection of IR radiation. We present a morphological characterization of the elytron, thermal imaging measurements and a theoretical IR model of insect elytron, uncovering a synergistic operation of all structures.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jtherbio.2021.102932DOI Listing
May 2021

Metagenomic Assembly: Reconstructing Genomes from Metagenomes.

Methods Mol Biol 2021 ;2242:139-152

Institute of Ecological Science, School of Life Science, South China Normal University, Guangzhou, Guangdong Province, China.

Assembly of metagenomic sequence data into microbial genomes is of critical importance for disentangling community complexity and unraveling the functional capacity of microorganisms. The rapid development of sequencing technology and novel assembly algorithms have made it possible to reliably reconstruct hundreds to thousands of microbial genomes from raw sequencing reads through metagenomic assembly. In this chapter, we introduce a routinely used metagenomic assembly workflow including read quality filtering, assembly, contig/scaffold binning, and postassembly check for genome completeness and contamination. We also describe a case study to reconstruct near-complete microbial genomes from metagenomes using our workflow.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/978-1-0716-1099-2_9DOI Listing
June 2021

Sulfonic-Group-Grafted TiCT MXene: A Silver Bullet to Settle the Instability of Polyaniline toward High-Performance Zn-Ion Batteries.

ACS Nano 2021 May 29;15(5):9065-9075. Epub 2021 Apr 29.

College of Materials Science and Engineering, Shenzhen University, Shenzhen 518055, China.

Polyaniline (PANI) is a promising cathode material for Zn-ion batteries (ZIBs) due to its intrinsic conductivity and redox activity; however, the achievements of PANI in high-performance ZIBs are largely hindered by its instability during the repeated charge/discharge. Taking advantage of the high conductivity, flexibility, and grafting ability together, a surface-engineered TiCT MXene is designed as a silver bullet to fight against the deprotonation and swelling/shrinking issues occurring in the redox process of PANI, which are the origins of its instability. Specifically, the sulfonic-group-grafted TiCT(S-TiCT) continuously provides protons to improve the protonation degree of PANI and maintains the polymer backbone at a locally low pH, which effectively inhibits deprotonation and brings high redox activity along with good reversibility. Meanwhile, the conductive and flexible natures of S-TiCT assist the fast redox reaction of PANI and concurrently buffer its corresponding swelling/shrinking. Therefore, the S-TiCT-enhanced PANI cathode simultaneously achieves a high discharge capacity of 262 mAh g at 0.5 A g, a superior rate capability of 160 mAh g at 15 A g, and a good cyclability over 5000 cycles with 100% coulombic efficiency. This work enlightens the development of versatile MXene surface engineering for advanced batteries.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsnano.1c02215DOI Listing
May 2021

RB1CC1 functions as a tumor-suppressing gene in renal cell carcinoma via suppression of PYK2 activity and disruption of TAZ-mediated PDL1 transcription activation.

Cancer Immunol Immunother 2021 Apr 10. Epub 2021 Apr 10.

Department of Urology, First Affiliated Hospital, University of South China, No. 69, Chuanshan Road, Hengyang, 421001, Hunan Province, People's Republic of China.

Rb1-inducible coiled-coil 1 (RB1CC1) has been demonstrated to function as an inhibitor of proline-rich/Ca-activated tyrosine kinase 2 (PYK2) by binding to the kinase domain of PYK2, which promotes the proliferation, invasion, and migration of renal cell carcinoma (RCC) cells. Additionally, in breast cancer, PYK2 positively regulates the expression of transcriptional co-activator with PDZ-binding motif (TAZ) which in turn can enhance PDL1 levels in breast and lung cancer cells. The current study was performed to decipher the impact of RB1CC1 in the progression of RCC via regulation of the PYK2/TAZ/PDL1 signaling axis. Expression of RB1CC1 and PYK2 was quantified in clinical tissue samples from RCC patients. The relationship between TAZ and PYK2, TAZ and PDL1 was then validated. The cellular processes of doxorubicin (DOX)-induced human RCC cell lines including the abilities of proliferation, colony formation, sphere formation and apoptosis, as well as the tumorigenicity of transfected cells, were evaluated after the alteration of RB1CC1 expression. RB1CC1 exhibited decreased expression in RCC tissues and was positively correlated with patient survival. RB1CC1 could inhibit the activity of PYK2, which in turn stimulated the stability of TAZ protein by phosphorylating TAZ. Meanwhile, TAZ protein activated PDL1 transcription by binding to the promoter region of PDL1. RB1CC1 overexpression or PYK2 knockdown could help everolimus (EVE) to inhibit tumor proliferation and activate immune response. Taken together, RB1CC1 can potentially augment the response of RCC cells to immunotherapy by suppressing the PYK2/TAZ/PDL1 signaling axis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00262-021-02913-8DOI Listing
April 2021

Number of Positive Lymph Nodes Is Superior to LNR and LODDS for Predicting the Prognosis of Pancreatic Neuroendocrine Neoplasms.

Front Endocrinol (Lausanne) 2021 5;12:613755. Epub 2021 Mar 5.

Department of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Background: The American Joint Committee on Cancer (AJCC) staging for pancreatic neuroendocrine neoplasms (PanNENs) based on the number of positive lymph nodes (PLNs) is the most widely accepted nodal staging system. New nodal staging schemes that take both the number of PLNs and the number of examined lymph nodes into consideration have emerged as useful prognostic tools. The aim of the current study was to determine the most effective nodal staging system, among the 8th edition AJCC N staging (or PLN staging), lymph node ratio (LNR), and log odds of positive lymph nodes (LODDS), for predicting the cause-specific survival of patients with PanNENs.

Methods: The clinicopathological and prognostic data of 2,295 patients from the Surveillance, Epidemiology, and End Results (SEER) database, diagnosed with PanNENs between 1988 and 2015, were reviewed retrospectively.

Results: A multivariate analysis identified PLN and LNR staging as independent prognostic factors, but not LODDS. The PLN staging exhibited higher C-index and area under the curve values than those of the LNR and LODDS, indicating better predictive discriminatory capacity. No significant difference in the survival of patients was observed within the same PLN staging subgroup according to the number (high or low) of examined lymph nodes. In contrast, intra-group heterogeneity was seen with use of LNR and LODDS staging, due to overestimation of the risk of insufficient examined lymph nodes, and LODDS failed to stratify patients without lymph nodes metastasis into different risk groups.

Conclusions: The PLN staging is more reliable than LNR and LODDS staging for predicting the cause-specific survival of PanNENs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fendo.2021.613755DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7977716PMC
March 2021

A bioinspired Au-CuS/CuS film with efficient low-angle-dependent and thermal-assisted photodetection properties.

iScience 2021 Mar 9;24(3):102167. Epub 2021 Feb 9.

Hunan Key Laboratory of Micro-Nano Energy Materials and Devices, Laboratory for Quantum Engineering and Micro-Nano Energy Technology, School of Physics and Optoelectronic, Xiangtan University, Xiangtan, Hunan 411105, China.

Inspired by the geological processes, this study develops an innovative low-concentration-ratio H reduction method to reduce the stoichiometric Au-CuS nanoparticles to produce completely reduced stoichiometric CuS with "invisible" Au achieved for solid solution Au enhancement. A stable Au-CuS/CuS micro/nano-composite is then formed by spontaneous oxidation. From this composite, in combination with biomimetic technology, an omnidirectional photoabsorption and thermoregulated film (Au-CuS/CuS-C-T_FW) is designed and fabricated as a photothermal-assisted and temperature-autoregulated photodetector for broadband and low-angle-dependent photodetection that presents good performance with high responsivity (26.37 mA/W), detectivity (1.25×10 Jones), and good stability at low bias (0.5 V). Solid solution Au exhibits significantly enhanced photodetection (1,000 times). This study offers a new concept for improving the stability and photoelectric properties of copper chalcogenides. Moreover, it opens up a new avenue toward enhancing the performance of optoelectronic and photovoltaic devices using solid solution metal atoms and thermal-assisted, anti-overheating temperature autoregulation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.isci.2021.102167DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7920830PMC
March 2021

A supramolecular complex of hydrazide-pillar[5]arene and bisdemethoxycurcumin with potential anti-cancer activity.

Bioorg Chem 2021 May 24;110:104764. Epub 2021 Feb 24.

School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, Jiangsu 225002, China. Electronic address:

Pillar[5]arene complexes of the naturally occurring compound bisdemethoxycurcumin (BDMC) were acquired for improving the water solubility and stability of BDMC. As a family member of curcuminoid compounds, BDMC has many interesting therapeutic properties. However, its low aqueous solubility and stability resulted in poor availability and restricted the clinical efficacy. Pillar[5]arenes with hydrophilic ends and a hydrophobic cavity could include with BDMC based on size matching. The synthesized hydrazide-pillar[5]arene (HP5A) and BDMC had a strong host-guest interaction with a 1:1 binding stoichiometry. Furthermore, the HP5A ⊃ BDMC complex could self-assemble into well-defined fibers in water/ethanol solution. This supramolecular complex worked well in vitro for inhibiting the proliferation of hepatoma carcinoma cells HepG2. Remarkably, this method of complexation with pillar[5]arenes visibly reduced the undesirable side effects on normal cells without weakening the anti-cancer activity of the drugs. We expected that the obtained host-guest complex and fibrous assembly would provide a promising platform for delivering drugs with low water solubility.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bioorg.2021.104764DOI Listing
May 2021

Effects of Circular RNA of Chicken Growth Hormone Receptor Gene on Cell Proliferation.

Front Genet 2021 11;12:598575. Epub 2021 Feb 11.

College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, China.

Animal growth and development are regulated by neural and endocrine growth axes, in which cell proliferation plays key roles. Recently, many research showed that circular RNAs were involved in hepatocyte and myoblast proliferation. Previously, we identified a circular RNA derived from the chicken gene, named circGHR. However, the function of circGHR is unclear. The objective of this study was to investigate circGHR expression pattern and its roles in cell proliferation. Results indicated that circGHR was a closed-loop structure molecule, and it was richer in the nucleus of hepatocytes and myoblast. Real-time PCR showed that circGHR was increased from E13 to the 7th week in the liver but decreased in the thigh and breast muscle. The CCK-8 assay displayed that circGHR promoted cell proliferation. Simultaneously, the biomarker genes , , and and the linear transcripts and were upregulated when circGHR was overexpressed. Altogether, these data exhibited that circGHR could promote cell proliferation possibly by regulating mRNA and expression.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fgene.2021.598575DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7905176PMC
February 2021

Abnormal expression of promotes the malignant behavior of glioma cells and leads to poor prognosis of glioma patients.

PeerJ 2021 8;9:e10820. Epub 2021 Feb 8.

Department of Surgery of Spine and Spinal Cord, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, School of Clinical Medicine, Henan University, Zhengzhou, Henan, China.

Background: Homeobox D11 () plays an important role in a variety of cancers, but its precise role in gliomas remains unclear. This study aimed to explore the relationship between and gliomas by combining bioinformatics methods with basic experimental validation.

Materials And Methods: Obtain gene expression information and clinical information of glioma and non-tumor brain tissue samples from multiple public databases such as TCGA (666 glioma samples), CGGA (749 glioma samples), GEPIA(163 glioblastoma samples and 207 normal control samples), GEO (GSE4290 and GSE15824). Nine cases of glioma tissue and five cases of normal control brain tissue were collected from the clinical department of Henan Provincial People's Hospital for further verification. A series of bioinformatic analysis methods were used to confirm the relationship between expression and overall survival and clinical molecular characteristics of patients with glioma. RT-qPCR was used to verify the change of expression level of in glioma cells and tissues. MTT assay, colony formation assay, wound-healing assay, immunofluorescence staining, flow cytometry and western blotting were used to detect the effect of on the biological behavior of glioma cell line U251.

Results: The high expression of was significantly related to age, World Health Organization (WHO) grade, chemotherapy status, histological type, and even 1p19q codeletion data and isocitrate dehydrogenase (IDH) mutation. , as an independent risk factor, reduces the overall survival of glioma patients and has diagnostic value for the prognosis of glioma. Gene Set Enrichment Analysis (GSEA) showed that was significantly enriched in cell signaling pathway such as cell cycle, DNA replication and so on. Finally, we confirmed that the knockout of can inhibit the proliferation and invasion of U251 glioma cells, and change the biological behavior of tumor cells by preventing the progression of cell cycle.

Conclusions: may be used as a candidate biomarker for the clinical application of targeted drug and prognostic assessment treatment of glioma. In addition, This study will help to explore the pathological mechanism of glioma.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7717/peerj.10820DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7877241PMC
February 2021

Association of D-dimer elevation with inflammation and organ dysfunction in ICU patients with COVID-19 in Wuhan, China: a retrospective observational study.

Aging (Albany NY) 2021 02 11;13(4):4794-4810. Epub 2021 Feb 11.

Department of Critical Care Medicine, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, P.R. China.

Coronavirus disease 2019 (COVID-19)-associated coagulation dysfunction is gaining attention. In particular, dynamic changes in the D-dimer level may be related to disease progression. Here, we explored whether elevated D-dimer level was related to multiple organ failure and a higher risk of death. This study included 158 patients with COVID-19 who were admitted to the intensive care unit (ICU) at Jinyintan Hospital in Wuhan, China between January 20, 2020 and February 26, 2020. Clinical and laboratory data were collected. The relationship between D-dimer elevation and organ dysfunction was analyzed, as were dynamic changes in inflammation and lipid metabolism. Approximately 63.9% of patients with COVID-19 had an elevated D-dimer level on ICU admission. The 14 day ICU mortality rate was significantly higher in patients with a high D-dimer level than in those with a normal D-dimer level. Patients with a D-dimer level of 10-40μg/mL had similar organ function on ICU admission to those with a D-dimer level of 1.5-10μg/mL. However, patients with higher levels of D-dimer developed organ injuries within 7 days. Furthermore, significant differences in inflammation and lipid metabolism markers were observed between the two groups. In conclusion, the D-dimer level is closely related to COVID-19 severity and might influence the likelihood of rapid onset of organ injury after admission.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.18632/aging.202496DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7950237PMC
February 2021

Fabrication and application of nanoporous anodic aluminum oxide: a review.

Nanotechnology 2021 Mar 10;32(22). Epub 2021 Mar 10.

State Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, People's Republic of China.

Due to the unique optical and electrochemical properties, large surface area, tunable properties, and high thermal stability, nanoporous anodic aluminum oxide (AAO) has become one of the most popular materials with a large potential to develop emerging applications in numerous areas, including biosensors, desalination, high-risk pollutants detection, capacitors, solar cell devices, photonic crystals, template-assisted fabrication of nanostructures, and so on. This review covers the mechanism of AAO formation, manufacturing technology, the relationship between the properties of AAO and fabrication conditions, and applications of AAO. Properties of AAO, like pore diameter, interpore distance, wall thickness, and anodized aluminum layer thickness, can be fully controlled by fabrication conditions, including electrolyte, applied voltage, anodizing and widening time. Generally speaking, the pore diameter of AAO will affect its specific application to a large extent. Moreover, manufacturing technology like one/two/multi step anodization, nanoimprint lithography anodization, and pulse/cyclic anodization also have a major impact on overall array arrangement. The review aims to provide a perspective overview of the relationship between applications and their corresponding AAO pore sizes, systematically. And the review also focuses on the strategies by which the structures and functions of AAO can be utilized.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1088/1361-6528/abe25fDOI Listing
March 2021

Templating Synthesis of Metal-Organic Framework Nanofiber Aerogels and Their Derived Hollow Porous Carbon Nanofibers for Energy Storage and Conversion.

Small 2021 Feb 1:e2004140. Epub 2021 Feb 1.

Hefei National Laboratory for Physical Sciences at the Microscale, CAS Center for Excellence in Nanoscience, Hefei Science Center of CAS, Collaborative Innovation Center of Suzhou Nano Science and Technology, Department of Chemistry, University of Science and Technology of China, Hefei, Anhui, 230026, P. R. China.

A kind of metal-organic framework (MOF) aerogels are synthesized by the self-assembly of uniform and monodisperse MOF nanofibers. Such MOF nanofiber aerogels as carbon precursors can effectively avoid the aggregation of nanofibers during calcination, resulting in the formation of well-dispersed hollow porous carbon nanofibers (HPCNs). Moreover, HPCNs with well-dispersion are investigated as sulfur host materials for Li-S batteries and electrocatalysts for cathode oxygen reduction reaction (ORR). On the one hand, HPCNs act as hosts for the encapsulation of sulfur into their hierarchical micro- and mesopores as well as hollow nanostructures. The obtained sulfur cathode exhibits excellent electrochemical features, good cycling stability and high coulombic efficiency. On the other hand, HPCNs exhibit better electrocatalytic activity than aggregated counterparts for ORR. Furthermore, a highly active single atom electrocatalyst can be prepared by the carbonization of bimetallic MOF nanofiber aerogels. The results indicate that well-dispersed HPCNs show enhanced electrochemical properties in contrast to their aggregated counterparts, suggesting that the dispersion situation of nanomaterials significantly influence their final performance. The present concept of employing MOF nanofiber aerogels as precursors will provide a new strategy to the design of MOF-derived nanomaterials with well-dispersion for their applications in energy storage and conversion.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/smll.202004140DOI Listing
February 2021

Evolution, Expression Profile, Regulatory Mechanism, and Functional Verification of EBP-Like Gene in Cholesterol Biosynthetic Process in Chickens (Gallus Gallus).

Front Genet 2020 14;11:587546. Epub 2021 Jan 14.

College of Animal Science, Henan Agricultural University, Zhengzhou, China.

The emopamil binding protein (EBP) is an important enzyme participating in the final steps of cholesterol biosynthesis in mammals. A predictive gene , which encodes the protein with a high identity to human EBP, was found in chicken genome. No regulatory mechanisms and biological functions of have been characterized in chickens. In the present study, the coding sequence of was cloned, the phylogenetic trees of EBP/EBP-like were constructed and the genomic synteny of was analyzed. The regulatory mechanism of were explored with and experiments. The biological functions of in liver cholesterol biosynthetic were examined by using gain- or loss-of-function strategies. The results showed that chicken gene was originated from a common ancestral with Japanese quail gene, and was relatively conservative with gene among different species. The gene was highly expressed in liver, its expression level was significantly increased in peak-laying stage, and was upregulated by estrogen. Inhibition of the mRNA expression could restrain the expressions of downstream genes (, , and ) in the cholesterol synthetic pathway, therefore downregulate the liver intracellular T-CHO level. In conclusion, as substitute of gene in chickens, plays a vital role in the process of chicken liver cholesterol synthesis. This research provides a basis for revealing the molecular regulatory mechanism of cholesterol synthesis in birds, contributes insights into the improvement of the growth and development, laying performance and egg quality in poultry.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fgene.2020.587546DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7841431PMC
January 2021
-->