Publications by authors named "Zhang Lei"

10,277 Publications

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Sensitivity enhancement in photothermal interferometry by balanced detection of the complex response to moving excitation.

Opt Lett 2021 Jun;46(12):2976-2979

The sensitivity of photothermal detection relies on both the magnitude of the response of a sample to excitation and the way the response is sensed. We propose a highly sensitive photothermal interferometry by addressing the above two issues. One is the use of moving excitation to enable a different manner in sample heating and cooling, which results in a strong thermoelastic response of the sample. The other is the use of a balanced Mach-Zehnder interferometer with a defocused probe beam to sense the complex response induced by the phase delays taking place at the sample surface and in the surrounding air. The method was verified experimentally with a Nd-doped glass to have 68-fold sensitivity enhancement over the classical photothermal common-path interferometry.
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http://dx.doi.org/10.1364/OL.426225DOI Listing
June 2021

Multi-level Attention Network for Retinal Vessel Segmentation.

IEEE J Biomed Health Inform 2021 Jun 15;PP. Epub 2021 Jun 15.

Automatic vessel segmentation in the fundus images plays an important role in the screening, diagnosis, treatment, and evaluation of various cardiovascular and ophthalmologic diseases. However, due to the limited well-annotated data, varying size of vessels, and intricate vessel structures, retinal vessel segmentation has become a long-standing challenge. In this paper, a novel deep learning model called AACA-MLA-D-UNet is proposed to fully utilize the low-level detailed information and the complementary information encoded in different layers to accurately distinguish the vessels from the background with low model complexity. The architecture of the proposed model is based on U-Net, and the dropout dense block is proposed to preserve maximum vessel information between convolution layers and mitigate the over-fitting problem. The adaptive atrous channel attention module is embedded in the contracting path to sort the importance of each feature channel automatically. After that, the multi-level attention module is proposed to integrate the multi-level features extracted from the expanding path, and use them to refine the features at each individual layer via attention mechanism. The proposed method has been validated on the three publicly available databases, i.e. the DRIVE, STARE, and CHASE DB1. The experimental results demonstrate that the proposed method can achieve better or comparable performance on retinal vessel segmentation with lower model complexity. Furthermore, the proposed method can also deal with some challenging cases and has strong generalization ability.
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http://dx.doi.org/10.1109/JBHI.2021.3089201DOI Listing
June 2021

Evaluation of efficiency and safety of oral corticosteroid therapy in children patients with exacerbations of asthma: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2021 Jun;100(24):e26250

Department of Pediatrics, the First People's Hospital of Jiangxia District.

Background: Asthma is the most frequently occurring obstructive airway disease, it inflicts the highest morbidity among children. Among the paediatric populace, severe exacerbations of asthma are a common reason behind patient consultations and hospitalizations. Oral corticosteroids are a primary component in the treatment of asthma exacerbations; however, there is controversy regarding how corticosteroids functions.

Methods: The present review will conduct a search on MEDLINE, EMBASE, Cochrane Library, China National Knowledge Infrastructure, and Chinese BioMedical Literature. The search will cover the databases from their beginning to May 2021. The search aims to identify all the randomized controlled studies on oral corticosteroids in treating children with asthma exacerbations. Two independent authors will choose studies, perform data extraction, and use an appropriate tool to assess the bias risk in the selected articles. Moreover, a sensitivity analysis will be performed to assess the robustness of the results. The RevMan (version 5.3) software will be employed to perform data synthesis and statistical analysis.

Results: This study will examine the efficiency and safeness of oral corticosteroid therapy to treat children with asthma exacerbations by pooling the results of individual studies.

Conclusion: The findings of this study will provide vigorous evidence to judge whether oral corticosteroid therapy is an efficiency strategy to treat patients with asthmatic exacerbations.

Osf Registration Number: May 20, 2021.osf.io/3ghjt. (https://osf.io/3ghjt/).
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http://dx.doi.org/10.1097/MD.0000000000026250DOI Listing
June 2021

One-Shot Neural Architecture Search by Dynamically Pruning Supernet in Hierarchical Order.

Int J Neural Syst 2021 Jun 14:2150029. Epub 2021 Jun 14.

College of Computer Science, Sichuan University, Section 4, Southern 1st Ring Rd, Chengdu, Sichuan 610065, P. R. China.

Neural Architecture Search (NAS), which aims at automatically designing neural architectures, recently draw a growing research interest. Different from conventional NAS methods, in which a large number of neural architectures need to be trained for evaluation, the one-shot NAS methods only have to train one supernet which synthesizes all the possible candidate architectures. As a result, the search efficiency could be significantly improved by sharing the supernet's weights during the candidate architectures' evaluation. This strategy could greatly speed up the search process but suffer a challenge that the evaluation based on sharing weights is not predictive enough. Recently, pruning the supernet during the search has been proven to be an efficient way to alleviate this problem. However, the pruning direction in complex-structured search space remains unexplored. In this paper, we revisited the role of path dropout strategy, which drops the neural operations instead of the neurons, in supernet training, and several interesting characters of the supernet trained with dropout are found. Based on the observations, a Hierarchically-Ordered Pruning Neural Architecture Search (HOPNAS) algorithm is proposed by dynamically pruning the supernet with a proper pruning direction. Experimental results indicate that our method is competitive with state-of-the-art approaches on CIFAR10 and ImageNet.
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http://dx.doi.org/10.1142/S0129065721500295DOI Listing
June 2021

Ultra-strong bio-glue from genetically engineered polypeptides.

Nat Commun 2021 06 14;12(1):3613. Epub 2021 Jun 14.

Zernike Institute for Advanced Materials, University of Groningen, Groningen, The Netherlands.

The development of biomedical glues is an important, yet challenging task as seemingly mutually exclusive properties need to be combined in one material, i.e. strong adhesion and adaption to remodeling processes in healing tissue. Here, we report a biocompatible and biodegradable protein-based adhesive with high adhesion strengths. The maximum strength reaches 16.5 ± 2.2 MPa on hard substrates, which is comparable to that of commercial cyanoacrylate superglue and higher than other protein-based adhesives by at least one order of magnitude. Moreover, the strong adhesion on soft tissues qualifies the adhesive as biomedical glue outperforming some commercial products. Robust mechanical properties are realized without covalent bond formation during the adhesion process. A complex consisting of cationic supercharged polypeptides and anionic aromatic surfactants with lysine to surfactant molar ratio of 1:0.9 is driven by multiple supramolecular interactions enabling such strong adhesion. We demonstrate the glue's robust performance in vitro and in vivo for cosmetic and hemostasis applications and accelerated wound healing by comparison to surgical wound closures.
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http://dx.doi.org/10.1038/s41467-021-23117-9DOI Listing
June 2021

A multifunctional nanocellulose-based hydrogel for strain sensing and self-powering applications.

Carbohydr Polym 2021 Sep 20;268:118210. Epub 2021 May 20.

Department of Chemical Engineering, University of New Brunswick, Fredericton, New Brunswick E3B 5A3, Canada. Electronic address:

Ionic conductive hydrogel with multifunctional properties have shown promising application potential in various fields, including electronic skin, wearable devices and sensors. Herein, a highly stretchable (up to 2800% strain), tough, adhesive ionic conductive hydrogel are prepared using cationic nanocellulose (CCNC) to disperse/stabilize graphitic carbon nitride (g-CN), forming CCNC-g-CN complexes and in situ radical polymerization process. The ionic interactions between CNCC and g-CN acted as sacrificial bonds enabled highly stretchability of the hydrogel. The hydrogel showed high sensitivity (gauge factor≈5.6, 0-1.6% strain), enabling the detection of human body motion, speech and exhalation. Furthermore, the hydrogel based self-powered device can charge 2.2 μF capacitor up to 15 V from human motion. This multifunctional hydrogel presents potential applications in self-powered wearable electronics.
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http://dx.doi.org/10.1016/j.carbpol.2021.118210DOI Listing
September 2021

Overcoming the Trade-off between Water Permeation and Mechanical Strength of Ceramic Membrane Supports by Interfacial Engineering.

ACS Appl Mater Interfaces 2021 Jun 14. Epub 2021 Jun 14.

Department of Material Science and Engineering, Faculty of Engineering, National University of Singapore, Singapore 117574, Singapore.

Porous ceramic membrane supports with high mechanical strength and permeation are required for highly permeable ceramic membranes. The water permeation of a ceramic membrane support is largely dependent on its level of open porosity, which can be however generally detrimental to the mechanical strength. In this work, low-cost kaolin nanoflakes were rationally composited with coarse alumina particles, and multichannel flat-sheet ceramic supports were successfully fabricated by extrusion and subsequent partial sintering. The macroscopic properties, microstructure characteristics, permeability, and mechanical strength of the ceramic membrane supports were systematically established and comprehensively studied. The incorporation of kaolin nanoflakes effectively reduced the sintering temperature to about 1200 °C. An interesting evolution of the pore structure was evidenced with the increase in sintering temperature. Interestingly, the porous ceramic supports prepared at 1400 °C with a nominal pore size of 1.47 μm showed the highest water permeability of 9911.9 ± 357.5 LMHB, and at the same time the flexural strength reached 109.6 ± 4.6 MPa. The much improved permeability was attributed to the unique multilevel pore structures, and the enhanced flexural strength mainly originated from the strongly interfacial bonding, as evidenced by the trans-granular fracture behavior. Also, the ceramic membrane supports exhibited excellent chemical resistance and good removal efficiency for oily wastewater. This work highlights the significant role of interfacial engineering in simultaneously improving the water permeation and mechanical strength, thereby overcoming their trade-off in porous ceramic membrane supports.
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http://dx.doi.org/10.1021/acsami.1c08157DOI Listing
June 2021

Plug-and-Play Image Restoration with Deep Denoiser Prior.

IEEE Trans Pattern Anal Mach Intell 2021 Jun 14;PP. Epub 2021 Jun 14.

Recent works on plug-and-play image restoration have shown that a denoiser can implicitly serve as the image prior for model-based methods to solve many inverse problems. Such a property induces considerable advantages for plug-and-play image restoration when the denoiser is discriminatively learned via deep convolutional neural network (CNN) with large modeling capacity. However, while deeper and larger CNN models are rapidly gaining popularity, existing plug-and-play image restoration hinders its performance due to the lack of suitable denoiser prior. In order to push the limits of plug-and-play image restoration, we set up a benchmark deep denoiser prior by training a highly flexible and effective CNN denoiser. We then plug the deep denoiser prior as a modular part into a half quadratic splitting based iterative algorithm to solve various image restoration problems. We, meanwhile, provide a thorough analysis of parameter setting, intermediate results and empirical convergence to better understand the working mechanism. Experimental results on three representative image restoration tasks, including deblurring, super-resolution and demosaicing, demonstrate that the proposed plug-and-play image restoration with deep denoiser prior not only significantly outperforms other state-of-the-art model-based methods but also achieves competitive or even superior performance against state-of-the-art learning-based methods. The source code is available: https://github.com/cszn/DPIR.
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http://dx.doi.org/10.1109/TPAMI.2021.3088914DOI Listing
June 2021

HDAC5 promotes intestinal sepsis via the Ghrelin/E2F1/NF-κB axis.

FASEB J 2021 Jul;35(7):e21368

Department of Respiratory, the First Hospital of Lanzhou University (the First School of Clinical Medicine), Lanzhou, P.R. China.

In the current study, we sought to determine the roles of histone deacetylase 5 (HDAC5) on the promotion of intestinal sepsis in a mouse model. Dual luciferase reporter gene assay was used to determine the binding relationship between HDAC5 and Ghrelin. Cecal ligation and puncture (CLP) was used as an animal model of intestinal sepsis. The roles of HDAC5 on intestinal sepsis were determined by HDAC5 knockdown, overexpression, and inhibitor (LMK-235) in vivo. Mice intestinal permeability and intestinal epithelial damage were evaluated, and HE staining was used to evaluate the intestinal mucosal injury index. Lipopolysaccharide (LPS)-treated intestinal-derived macrophages served as a cell model of sepsis, followed by the loss-of-function and gain-of-function assays. ELISA was used to determine the levels of inflammatory factors, and TUNEL staining was used to detect intestinal cell apoptosis. HDAC5 was upregulated in the intestine of sepsis patients. This increased HDAC5 expression was positively correlated with the expression of inflammatory factors TNF-α, IL-1β, IL-6, and HMGB1, as well as the intestinal dysfunction-related factors IFABP. In sepsis mice, the expression of inflammatory factors was reduced by HDAC5 knockdown. HDAC5 knockdown also improved survival, morphology of intestinal tissue, intestinal permeability, and epithelial damage. Ghrelin was bound and inhibited by HDAC5, but E2F1 expression was increased by Ghrelin overexpression, leading to inhibition of the NF-κB pathway. Ghrelin and E2F1 expression were increased by the treatment with HDAC5 inhibitor LMK-235, which inhibited the NF-κB pathway to improve intestinal dysfunction in the sepsis model. In conclusion, HDAC5 inhibits Ghrelin to reduce E2F1 and thus activate the NF-κB pathway, thereby promoting intestinal sepsis.
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http://dx.doi.org/10.1096/fj.202001584RDOI Listing
July 2021

Effect of Local Delivery of Vancomycin and Tobramycin on Bone Regeneration.

Orthop Surg 2021 Jun 14. Epub 2021 Jun 14.

Department of Traumatology, Beijing Jishuitan Hospital, Beijing, China.

Objective: A bone defect rat model was established to investigate the osteogenic effect of local delivery two antibiotics (vancomycin and tobramycin powder) on bone regeneration.

Methods: Twenty-four Sprague-Dawley (SD) male rats (6 to 8 weeks, 200 to 250 g) were used in this study. All these rats were randomly divided into four groups. Based on dose conversion between rat and human via body surface area, the rat dose of two antibiotics was 88μg/g and 176 μg/g for vancomycin and tobramycin, respectively. Con group (no antibiotic), Van group (vancomycin, 88 μg/g), Tob group (tobramycin 176 μg/g), and Van+Tob group (vancomycin 88μg/g combined with tobramycin 176 μg/g). A 5.0-mm full-thickness standardized mandibular bone defect was performed with a drill in each rat and different antibiotic powders were placed over the bone defect space, respectively. All these animals were sacrificed after 12 weeks post-operation. The mandible bones were harvested for further radiographic and histologic analysis. The bone volume/total volume (BV/TV) ratio, bone volume (BV), and bone fractional area (BFA) in the defect area via micro-computed tomography (μCT scanning) were further analyzed. Then, we performed a histological assessment via hematoxylin and eosin (H&E) and Masson's trichrome staining to analyze bone regeneration and also analyze the number of osteoblasts per filed.

Results: There were no postoperative deaths, signs of vancomycin-related or tobramycin-related toxicity, or signs of systemic illness in any of the four groups. All wounds healed well, and no complications or surgical site infection were observed in all rats. From the μCT scans analyses, there was less bone regeneration in the Van group than in the Con group (BV/TV: F = 64.29, R  = 0.9602; P = 0.0052; BFA: F = 76.17, R  = 0.9662, P = 0.0007; BV: F = 194.4, R  = 0.9865, P = 0.0022). However, when the tobramycin and vancomycin were combined, an increase in bone defect re-ossification was found in the Van+Tob group than in the Van group (BV/TV: F = 64.29, R  = 0.9602, P = 0.0033; BFA: F = 76.17, R  = 0.9662, P = 0.0006; BV: F = 194.4, R  = 0.9865, P = 0.0033). Routine H&E and Masson staining supported the finding of μCT scanning. Quantitative indices confirmed that both the bone regeneration and the number of osteoblasts per filed in the defect area was higher in the Van+Tob group than in the Van group (percentage of bone tissue: F = 145.7, R  = 0.9562, P = 0.0008; number of osteoblasts per file; F = 67.3, R  = 0.9098, P < 0.0001). There was no significant difference between the Con group and the Van+Tob group on the number of osteoblasts each field (F = 145.7, R  = 0.9562, P > 0.9999).

Conclusion: For bone defect, local application of vancomycin combined with tobramycin was recommended over vancomycin alone. This animal study presents data suggesting that the use of local delivery of vancomycin and tobramycin should be investigated further in clinical studies.
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http://dx.doi.org/10.1111/os.13020DOI Listing
June 2021

Investigation on the Distribution of Yimin Lignite Pyrolysis Products and the Stability of its Char.

ACS Omega 2021 Jun 21;6(22):13953-13961. Epub 2021 May 21.

China University of Mining and Technology, Beijing 100083, China.

This paper introduces the utilization of lignite in China and abroad and studies the influence of different process conditions on the pyrolysis products of lignite. The effects of pyrolysis temperature, residence time, and heating rate on the yield and stability of pyrolysis products were studied by standard lattice low-temperature distillation of coal. The results showed that the final pyrolysis temperature of lignite increases gradually, which is a key factor affecting the pyrolysis performance of lignite. At the same time, the combustible gas yield and tar yield were also significantly improved. Semichar yield and semichar volatile content showed a downward trend. From the range of pyrolysis products, the heating rate also has an important influence on the pyrolysis performance of lignite. Through the thermal stability test of lignite, it is concluded that the particle size distribution of carbon black products is not significantly different, and most of the coal particles are mainly distributed in the range of more than 6 mm.
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http://dx.doi.org/10.1021/acsomega.0c05204DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8190781PMC
June 2021

Ginsenoside Rb Enhances Plaque Stability and Inhibits Adventitial Vasa Vasorum via the Modulation of miR-33 and PEDF.

Front Cardiovasc Med 2021 28;8:654670. Epub 2021 May 28.

The Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education, Chinese National Health Commission and Chinese Academy of Medical Sciences, The State and Shandong Province Joint Key Laboratory of Translational Cardiovascular Medicine, Department of Cardiology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China.

Atherosclerosis is closely associated with proliferation of the adventitial vasa vasorum, leading to the atherosclerotic plaque progression and vulnerability. In this report, we investigated the role of Ginsenoside Rb (Rb) on atherosclerotic plaque stabilization and adventitial vasa vasorum (VV) along with the mechanisms involved. Apolipoprotein E-deficient (ApoE) mice were fed with a high-fat diet for 20 weeks, and then Ginsenoside Rb (50 mg/kg/d, intraperitoneal) was given for 4 weeks. Rb treatment significantly inhibited adventitial VV proliferation, alleviated inflammation, decreased plaque burden, and stabilized atherosclerotic plaques in apoE mice. However, the beneficial effects of Rb on atherosclerotic lesion was attenuated by overexpression of miR-33. The analysis from atherosclerotic plaque revealed that Rb treatment could result in an induction of Pigment epithelium-derived factor (PEDF) expression and reduction of the miR-33 generation. Overexpression of miR-33 significantly reverted the Rb-mediated elevation of PEDF and anti-angiogenic effect. Ginsenoside Rb attenuates plaque growth and enhances plaque stability partially through inhibiting adventitial vasa vasorum proliferation and inflammation in apoE mice. The anti-angiogenic and anti-inflammation effects of Rb are exerted via the modulation of miR-33 and its target gene PEDF.
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http://dx.doi.org/10.3389/fcvm.2021.654670DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8192703PMC
May 2021

Neutrophil-to-Lymphocyte and Platelet-to-Lymphocyte Ratios as Predictors of Outcomes in Patients With Unresectable Hepatocellular Carcinoma Undergoing Transarterial Chemoembolization Plus Sorafenib.

Front Mol Biosci 2021 28;8:624366. Epub 2021 May 28.

Department of Radiology, The Affiliated People's Hospital of Ningbo University, Ningbo, China.

To investigate the predictive value of inflammatory biomarkers in patients with unresectable hepatocellular carcinoma (HCC) for outcomes following the combination treatment of transarterial chemoembolization (TACE) plus sorafenib. A total of 314 (270 male and 44 female) treatment-naïve patients with unresectable HCC treated by TACE plus sorafenib between January 2011 and December 2018 were enrolled in the retrospective study. The primary outcome was overall survival (OS). The secondary outcome was progression-free survival (PFS). Neutrophil-to-lymphocyte ratio (NLR) and platelet-to-lymphocyte ratio (PLR) were obtained within 3-7 days before the initial TACE and the median value of the NLR and PLR was considered as the cut-off value. The median value of NLR and PLR was 2.42 and 100, respectively. The median OS and PFS of the entire cohort were 18.7 months (95% CI: 16.8-20.6) and 9.1 months (95% CI: 8.5-9.8), respectively. The low NLR and PLR group showed improved OS and PFS compared with the high NLR and PLR group [21.8 months (95% CI: 15.2-28.5) vs. 15.4 months (95% CI: 12.4-18.3), < 0.0001; 21.6 months (95% CI: 15.8-27.5) vs. 14.9 months (95% CI: 11.9-17.8), = 0.00027, respectively]. In addition, the low NLR and PLR group also provided a longer PFS than the high NLR and PLR group [10.4 months (95% CI: 8.9-12.0) vs. 8.1 months (95% CI: 7.1-9.2), = 0.00022; 10.3 months (95% CI: 8.6-11.9) vs. 8.2 months (95% CI: 7.2-9.2), < 0.0001, respectively]. High NLR and PLR at baseline were predictive factors of poor OS ( = 0.02 and = 0.004) and PFS ( = 0.045 and = 0.005). This study showed the prognostic value of quantitative inflammatory biomarkers in correlation with OS and PFS in unresectable HCC patients undergoing TACE plus sorafenib treatment.
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http://dx.doi.org/10.3389/fmolb.2021.624366DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8194392PMC
May 2021

Anodic oxidation triggered divergent 1,2- and 1,4-group transfer reactions of β-hydroxycarboxylic acids enabled by electrochemical regulation.

Chem Sci 2020 Sep 28;11(44):12021-12028. Epub 2020 Sep 28.

Department of Energy and Resources Engineering, College of Engineering, Peking University Beijing 100871 China

We report a set of electrochemically regulated protocols for the divergent synthesis of ketones and β-keto esters from the same β-hydroxycarboxylic acid starting materials. Enabled by electrochemical control, the anodic oxidation of carboxylic acids proceeded in either a one-electron or a two-electron pathway, leading to a 1,4-aryl transfer or a semipinacol-type 1,2-group transfer product with excellent chemoselectivity. The 1,4-aryl transfer represents an unprecedented example of carbon-to-oxygen group transfer proceeding a radical mechanism. In contrast to previously reported radical group transfer reactions, this 1,4-group transfer process features the migration of electron-rich aryl substituents. Furthermore, with these chemoselective electrochemical oxidation protocols, a range of ketones and β-keto esters including those possessing a challenging-to-access medium-sized ring could be synthesized in excellent yields.
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http://dx.doi.org/10.1039/d0sc02386hDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8162457PMC
September 2020

Designed multifunctional ratiometric fluorescent probe for directly detecting fluoride ion/ dichromate and indirectly monitoring urea.

J Hazard Mater 2021 Jun 5;418:126271. Epub 2021 Jun 5.

College of Chemistry, Liaoning University, 66 Chongshan Middle Road, Shenyang, Liaoning 110036, People's Republic of China. Electronic address:

[email protected] Y composite was obtained by confining eosin Y (EY) into the cavities of Zr-MOF and could emit two fluorescence peaks at 453 and 543 nm at an excitation wavelength of 355 nm. This multi-responsive and multifunctional ratiometric fluorescent nanoprobe not only enable directly distinct detection of F/CrO with ultra-high selectivity and sensitivity, but also could indirectly monitor the concentration of urea based on unique enzymatic hydrolysis reaction. The multifunctional probe was utilized for fluorescence labeling F-/CrO in sweat latent fingerprint through an environmentally friendly powder strategy and exhibited obvious luminescence visualization changes. Notably, the corresponding portable on-line test strips of probe for detection of F and CrO were made for monitoring the levels of F and CrO. Furthermore, the probe was applied to evaluate the degrees of F/CrO in HepG-2 cell and urea in serum with superior results,which indicate the potential application of the as-synthesized [email protected] as multifunctional probe for the detection of F, CrO and urea in biological samples. Finally, in order to extend the device-based applications of probe, an AND-OR-coupled molecular logic gate was put on agenda.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126271DOI Listing
June 2021

Spatial distribution and source of biotoxins in phytoplankton from the South China Sea, China.

J Hazard Mater 2021 Jun 3;418:126285. Epub 2021 Jun 3.

College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China; Key Laboratory of Marine Environment and Ecology, Ocean University of China, Ministry of Education, Qingdao 266100, China. Electronic address:

Marine phycotoxins severely threaten ecosystem health and mariculture. This study investigates the spatial distribution and source of diverse phycotoxins in the South China Sea (SCS), during four 2019/2020 cruises. Saxitoxin (STX) and okadaic acid (OA) -groups, azaspiracids, cyclic imines, pectenotoxins (PTX), yessotoxins, and domoic acid (DA) toxins were analyzed in microalgal samples. PTX2 occurred with the highest (93.5%) detection rate (DR) during all cruises, especially in the Pearl River Estuary (PRE) in June 2019. Homo-yessotoxin (hYTX) and DA were found during three cruises in August 2020, and high DR of hYTX (67.7%, 29.3%) and DA (29.0%, 29.3%) in the PRE and Guangdong coast, respectively, in June 2019 and 2020, peaking at concentrations of 777 pg hYTX L and 38514 pg DA L. The phycotoxin distribution demonstrated that DA-producing microalgae gathered close to the PRE and Guangdong coast, while hYTX-producing microalgae distributed relatively far offshore. Microalgae producing PTX2- and STX-group toxins were more widely living in the SCS. High-throughput sequencing results suggested that Alexandrium pacificum and Gonyaulax spinifera were responsible for STX-group toxins and hYTX, respectively, while Pseudo-nitzschia cuspidata was the main source of DA. Widely distributed PTX2, hYTX, and DA were reported for the first time in the SCS.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126285DOI Listing
June 2021

Molecular simulation of nano polyhedral oligomeric silsesquioxane doping effect on the properties of two-component crosslinked epoxy resin.

J Mol Graph Model 2021 Jun 7;107:107961. Epub 2021 Jun 7.

State Key Laboratory of Power Transmission Equipment & System Security and New Technology, Chongqing University, Chongqing, China.

Epoxy resin (EP) has been extensively used in the field of insulation for its excellent electrical strength, mechanical property, chemical stability, and low cost. In this paper, computer molecular simulation is used to analyze the influence of nano-POSS (Nano-Polyhedral Oligomericsils Esquioxane) doping on the properties of epoxy composite from the micro point of view, which can provide a scientific basis for the optimization of the epoxy system. Two kinds of nano-POSS fillers with different mass fractions were doped into the base material of diglycidyl ether of bisphenol A (DGEBA) and 3,4-epoxycyclohexylmethyl 3,4-epoxycyclohexanecar (OSC). By molecular dynamics (MD) simulation the influence of nanofillers on the microstructure, thermal and mechanical properties of the composite were analyzed. Simulation results show that the doping of nano-POSS can improve the thermal and mechanical properties of the epoxy composite. Different nano-POSS has little effect on the glass transition temperature (T), coefficient of thermal expansion (CTE), and mechanical properties of the epoxy system, while the filling amount has an obvious improvement effect. Compared with EP/methyl-POSS system, the thermal and mechanical properties of the EP/phenyl-POSS system are better. At the same time, the doping of nano-POSS changed the microstructure parameters of epoxy composite. With the increase of nano-POSS filler content, fractional free volume (FFV) and mean square displacement (MSD) of both EP/POSS systems increased after the first drop. Besides, when the content of nano-POSS exceeded a certain range, the aggregation of filler itself hindered the accumulation of epoxy molecular chain segments.
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http://dx.doi.org/10.1016/j.jmgm.2021.107961DOI Listing
June 2021

The modification of T description according to visceral pleural invasion and tumor size from 3.1 cm to 4.0 cm in non-small cell lung cancer: A retrospective analysis based on the SEER database.

Lung Cancer 2021 Jun 10;158:47-54. Epub 2021 Jun 10.

Department of Thoracic Surgery, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China. Electronic address:

Objectives: As a poor prognostic factor, visceral pleural invasion (VPI) was incorporated into non-small cell lung cancer (NSCLC) staging system. For modifying the T description of NSCLC, the prognostic value of VPI was assessed.

Materials And Methods: From 2010-2015, data on stage pT2N0M0 NSCLC patients with tumor size (TS) from 3.1 cm to 5.0 cm who received surgery from the Surveillance, Epidemiology, and End Results (SEER) database were enrolled retrospectively. Propensity score matching was utilized to balance the baseline factors according to different TS intervals. Overall survival (OS) was assessed by the Kaplan-Meier method and log-rank test. Univariate and multivariate analysis were applied to identify the prognostic factors. The risk factors of VPI were calculated by logistic regression model.

Result: The sum of 4005 resected stage pT2N0M0 NSCLC patients with TS from 3.1 cm to 5.0 cm were recruited, which had 1084 patients with VPI and 2921 patients without VPI respectively. As TS interval of 3.1-4.0 cm, the 5-year OS of patients without VPI was significantly better than those with VPI (62.6 % vs 58.7 %, P = 0.015), while the 5-year OS of patients with VPI and TS interval of 3.1-4.0 cm had no significant difference compared with patients whose TS interval of 4.1-5.0 cm (58.7 % vs 58.8 %, P = 0.918). Logistic regressive analysis manifested that older age, female, worse differentiation grade and larger TS had higher incidence of VPI (OR = 1.01, 1.25, 1.25, 1.16, respectively; P < 0.05 for all).

Conclusion: This study underlined the prognostic effect of VPI and suggested that early-stage NSCLC with VPI and TS interval of 3.1-4.0 cm could be appropriately upstaged from pT2a (stage pIB) to pT2b (modified stage pIIA).
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http://dx.doi.org/10.1016/j.lungcan.2021.06.003DOI Listing
June 2021

Brain structural alterations in MDD patients with gastrointestinal symptoms: Evidence from the REST-meta-MDD project.

Prog Neuropsychopharmacol Biol Psychiatry 2021 Jun 10:110386. Epub 2021 Jun 10.

Department of Psychiatry, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.

Objective: While gastrointestinal (GI) symptoms are very common in patients with major depressive disorder (MDD), few studies have investigated the neural basis behind these symptoms. In this study, we sought to elucidate the neural basis of GI symptoms in MDD patients by analyzing the changes in regional gray matter volume (GMV) and gray matter density (GMD) in brain structure.

Method: Subjects were recruited from 13 clinical centers and categorized into three groups, each of which is based on the presence or absence of GI symptoms: the GI symptoms group (MDD patients with at least one GI symptom), the non-GI symptoms group (MDD patients without any GI symptoms), and the healthy control group (HCs). Structural magnetic resonance images (MRI) were collected of 335 patients in the GI symptoms group, 149 patients in the non-GI symptoms group, and 446 patients in the healthy control group. The 17-item Hamilton Depression Rating Scale (HAMD-17) was administered to all patients. Correlation analysis and logistic regression analysis were used to determine if there was a correlation between the altered brain regions and the clinical symptoms.

Results: There were significantly higher HAMD-17 scores in the GI symptoms group than that of the non-GI symptoms group (P < 0.001). Both GMV and GMD were significant different among the three groups for the bilateral superior temporal gyrus, bilateral middle temporal gyrus, left lingual gyrus, bilateral caudate nucleus, right Fusiform gyrus and bilateral Thalamus (GRF correction, cluster-P < 0.01, voxel-P < 0.001). Compared to the HC group, the GI symptoms group demonstrated increased GMV and GMD in the bilateral superior temporal gyrus, and the non-GI symptoms group demonstrated an increased GMV and GMD in the right superior temporal gyrus, right fusiform gyrus and decreased GMV in the right Caudate nucleus (GRF correction, cluster-P < 0.01, voxel-P < 0.001). Compared to the non-GI symptoms group, the GI symptoms group demonstrated significantly increased GMV and GMD in the bilateral thalamus, as well as decreased GMV in the bilateral superior temporal gyrus and bilateral insula lobe (GRF correction, cluster-P < 0.01, voxel-P < 0.001). While these changed brain areas had significantly association with GI symptoms (P < 0.001), they were not correlated with depressive symptoms (P > 0.05). Risk factors for gastrointestinal symptoms in MDD patients (p < 0.05) included age, increased GMD in the right thalamus, and decreased GMV in the bilateral superior temporal gyrus and left Insula lobe.

Conclusion: MDD patients with GI symptoms have more severe depressive symptoms. MDD patients with GI symptoms exhibited larger GMV and GMD in the bilateral thalamus, and smaller GMV in the bilateral superior temporal gyrus and bilateral insula lobe that were correlated with GI symptoms, and some of them and age may contribute to the presence of GI symptoms in MDD patients.
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http://dx.doi.org/10.1016/j.pnpbp.2021.110386DOI Listing
June 2021

An integrated gene catalog and over 10,000 metagenome-assembled genomes from the gastrointestinal microbiome of ruminants.

Microbiome 2021 Jun 12;9(1):137. Epub 2021 Jun 12.

Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.

Background: Gastrointestinal tract (GIT) microbiomes in ruminants play major roles in host health and thus animal production. However, we lack an integrated understanding of microbial community structure and function as prior studies. are predominantly biased towards the rumen. Therefore, to acquire a microbiota inventory of the discrete GIT compartments, In this study, we used shotgun metagenomics to profile the microbiota of 370 samples that represent 10 GIT regions of seven ruminant species.

Results: Our analyses reconstructed a GIT microbial reference catalog with > 154 million nonredundant genes and identified 8745 uncultured candidate species from over 10,000 metagenome-assembled genomes. The integrated gene catalog across the GIT regions demonstrates spatial associations between the microbiome and physiological adaptations, and 8745 newly characterized genomes substantially expand the genomic landscape of ruminant microbiota, particularly those from the lower gut. This substantially expands the previously known set of endogenous microbial diversity and the taxonomic classification rate of the GIT microbiome. These candidate species encode hundreds of enzymes and novel biosynthetic gene clusters that improve our understanding concerning methane production and feed efficiency in ruminants. Overall, this study expands the characterization of the ruminant GIT microbiota at unprecedented spatial resolution and offers clues for improving ruminant livestock production in the future.

Conclusions: Having access to a comprehensive gene catalog and collections of microbial genomes provides the ability to perform efficiently genome-based analysis to achieve a detailed classification of GIT microbial ecosystem composition. Our study will bring unprecedented power in future association studies to investigate the impact of the GIT microbiota in ruminant health and production. Video abstract.
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http://dx.doi.org/10.1186/s40168-021-01078-xDOI Listing
June 2021

Prevalence and risk factors for bone loss in rheumatoid arthritis patients from South China: modeled by three methods.

BMC Musculoskelet Disord 2021 Jun 12;22(1):534. Epub 2021 Jun 12.

Division of Rheumatology, Zhuhai Hospital of Guangdong Provincial Hospital of Chinese Medicine, No.53, Ji'Da Jingle Road, Xiangzhou District, Zhuhai City, 519015, China.

Background: To explore the prevalence of bone loss among patients with rheumatoid arthritis (RA) and healthy controls (HC) and further explored the risk factors for osteopenia and osteoporosis of RA patients.

Methods: A cross-sectional survey was undertaken in four hospitals in different districts in South China to reveal the prevalence of bone loss in patients. Case records, laboratory tests, and bone mineral density (BMD) results of patients were collected. Traditional multivariable logistic regression analysis and two machine learning methods, including least absolute shrinkage selection operator (LASSO) and random forest (RF) were for exploring the risk factors for osteopenia or osteoporosis in RA patients.

Results: Four hundred five patients with RA and 198 HC were included. RA patients had lower BMD in almost BMD measurement sites than healthy controls; the decline of lumbar spine BMD was earlier than HC. RA patients were more likely to comorbid with osteopenia and osteoporosis (p for trend < 0.001) in the lumbar spine than HC. Higher serum 25-hydroxyvitamin D3 level and using tumor necrosis factor inhibitor in the last year were protective factors; aging, lower body mass index, and increased serum uric acid might be risk factors for bone loss.

Conclusions: RA patients were more prone and earlier to have bone loss than HC. More attention should be paid to measuring BMD in RA patients aging with lower BMI or hyperuricemia. Besides, serum vitamin D and all three measurement sites are recommended to check routinely. TNFi usage in the last year might benefit bone mass.
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http://dx.doi.org/10.1186/s12891-021-04403-5DOI Listing
June 2021

Incidence and risk factors of late-onset hemorrhagic cystitis after single umbilical cord blood transplantation with myeloablative conditioning regimen.

Int J Hematol 2021 Jun 12. Epub 2021 Jun 12.

Department of Hematology of Anhui Provincial Hospital, Anhui Medical University, Hefei, China.

Objective: To explore the incidence and risk factors of late-onset hemorrhagic cystitis (LOHC) in patients undergoing single umbilical cord blood transplantation for hematological malignancies.

Methods: Clinical data from 234 patients who consecutively underwent single UCBT using a myeloablative conditioning regimen without antithymocyte globulin in our center were retrospectively analyzed.

Results: In total, 64 (27.4%) patients developed LOHC with a median onset time of 40.5 (range 8-154) days, and 15 (6.4%) patients gradually developed grade III-IV LOHC. The incidence of LOHC was marginally higher in adults (31.0%) than in children (23.7%) (p = 0.248). HLA matching ≤ 6/8 (HR = 2.624, 95% CI 1.112-6.191, p = 0.028) was an independent risk factor for LOHC. The overall survival of LOHC patients (59.8%, 95% CI 61.7-85.5%) was significantly lower than that of patients without LOHC (86.8%, 95% CI 79.6-91.6%) at 130 days post transplantation (p = 0.036).

Conclusion: Patients with less well-matched grafts have a higher incidence of LOHC. Inherent deficiencies in immunity in the context of HLA disparity and more intense pharmacologic immunosuppression after severe acute graft-versus-host disease may contribute to viral activation. Prevention and treatment of LOHC have the potential to prolong long-term survival.
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http://dx.doi.org/10.1007/s12185-021-03168-wDOI Listing
June 2021

VE-cadherin N-glycosylation modified by GnT-V regulates VE-cadherin/β-catenin interaction and monocyte adhesion.

Exp Physiol 2021 Jun 12. Epub 2021 Jun 12.

College of Pharmacy, Chongqing Medical University, Chongqing, 400016, P.R. China.

New Findings: What is the central question of this study? Inflammation-induced monocyte adhesion is the initiator of most vascular diseases. The underlying mechanisms mediating monocyte adhesion remain to be fully clarified. What is the main finding and its importance? GnT-V medicated N-glycosylation on VE-cadherin regulates the dissociation of VE-cadherin/β-catenin complex to modulate the monocyte adhesion, but GnT-V overexpression can not rescue monocyte adhesion induced by IL-1β. This study enriched the molecular mechanism of VE-cadherin in regulating the monocyte adhesion process.

Abstract: Monocyte adhesion is a critical step in the initial stage of atherosclerosis, and dysfunction of VE-cadherin was reported to be involved in this process. Our group has found that VE-cadherin and its binding protein, β-catenin, were modified by sialylation, and the sialylation levels were both decreased in proinflammatory cytokine-treated EA.hy926 cells. In this study, we further confirmed the sugar chains of on VE-cadherin were modified by GnT-V. Results showed that the levels of GnT-V and β1,6 GlcNAc on the VE-cadherin were decreased in the presence of IL-1β, while the level of monocyte transendothelial migration was increased. Moreover, the interaction between VE-cadherin and β-catenin was increased, accompanied by an increased accumulation of degradative VE-cadherin and cytoplasmic β-catenin, indicating the impairment of cell-cell junction after IL-1β treatment. Furthermore, GnT-V shRNA and overexpression analysis confirmed that glycosylation of VE-cadherin was modified by GnT-V in EA. hy926 cells, which contributed to the monocyte-endothelial adhesion process. Taken together, these results suggested that the function of VE-cadherin in facilitating monocyte adhesion might result from the decreasing GnT-V expression and GnT-V-catalyzed N-glycosylation disorder. Our study enriched the molecular mechanism of VE-cadherin in regulating the monocyte adhesion process, and opened new insights into the post-transcriptional modifications on VE-cadherin. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1113/EP089617DOI Listing
June 2021

Distinct basal brain functional activity and connectivity in the emotional-arousal network and thalamus in patients with functional constipation associated with anxiety and/or depressive disorders.

Psychosom Med 2021 Jun 11. Epub 2021 Jun 11.

Center for Brain Imaging, School of Life Science and Technology, Xidian University, Xi'an, Shaanxi 710071, China State Key Laboratory of Cancer Biology, National Clinical Research Center for Digestive Diseases and Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi'an, Shaanxi 710032, China Department of Radiology, Tangdu Hospital, the Fourth Military Medical University, Xi'an, Shaanxi 710038, China Department of Psychiatry, Xijing Hospital, the Fourth Military Medical University, Xi'an, Shaanxi 710032, China.

Objective: Functional constipation (FC) is a common gastrointestinal disorder. Anxiety and/or depressive disorders are common in patients with FC (FCAD). Brain dysfunction may play a role in FC, but the contribution of comorbid anxiety and/or depression in patients with FC is poorly understood.

Methods: Sixty-five FC patients and forty-two healthy controls (HC) were recruited, and hierarchical-clustering algorithm was used to classify FC patients into FCAD and patients without anxiety/depressive status (FCNAD) based on neuropsychological assessment. Resting-state functional Magnetic Resonance Imaging measures including fractional amplitude of low-frequency fluctuation (fALFF) and functional connectivity were employed to investigate brain functional differences.

Results: 37 patients were classified as FCAD and 28 patients were classified as FCNAD; both groups showed decreased activity (fALFF) than HC in the perigenual anterior cingulate cortex (pACC), dorsomedial prefrontal cortex (DMPFC) and precuneus; enhanced precentral gyrus (PreCen)-thalamus connectivity and attenuated precuneus-thalamus connectivity in FCAD/FCNAD highlighted the thalamus as a critical connectivity node in the brain network (PFWE < .05). FCAD also had decreased fALFF than FCNAD/HC in the orbitofrontal cortex (OFC) and thalamus, and increased OFC-hippocampus connectivity. In the FCNAD group, brain activities (pACC/DMPFC) and connection (precuneus-thalamus) had correlations only with symptoms; in the FCAD group, brain activities (OFC, pACC/DMPFC) and connectivities (OFC-hippocampus/PreCen-thalamus) showed correlations with both constipation symptoms and anxiety/depressive status ratings. Mediation analysis indicated the relationship between abdominal distension and OFC activity was completely mediated by anxiety in FCAD.

Conclusions: These findings provide evidence of differences in brain activity and functional connectivity between FCAD and FCNAD. It might help portray important clues for improving new treatment strategies.
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http://dx.doi.org/10.1097/PSY.0000000000000958DOI Listing
June 2021

Multi-scale hierarchical scaffolds with aligned micro-fibers for promoting cell alignment.

Biomed Mater 2021 Jun 11. Epub 2021 Jun 11.

Tsinghua University, Tsinghua University, Beijing, Beijing, 100084, CHINA.

Cell alignment plays an essential role in cytoskeleton reorganization, extracellular matrix remodeling, and biomechanical properties regulation of tissues such as vascular tissues, cardiac muscles, and tendons. Based on the natural-oriented features of cells in native tissues, various biomimetic scaffolds have been reported with the introduction of well-arranged ultrafine fibers to induce cell alignment. However, it is still a challenge to fabricate scaffolds with suitable mechanical properties, biomimetic microenvironment, and ability to promote cell alignment. In this paper, we propose an integrated 3D printing system to fabricate multi-scale hierarchical scaffolds combined with meso-, micro-, and nano-fibrous filaments, in which the meso-, micro-, and nano-fibres fabricated via fused deposition modeling, melt electrospining writing, and solution electrospining can provide structural support, promote cell alignment, and create a biomimetic microenvironment to facilitate cell function, respectively. The plasma surface modification was performed improve the surface wettability of the scaffolds by measuring the contact angle. The obtained in vitro biological results validate the ability of multi-scale hierarchical scaffolds to enhance cell adhesion and proliferation, and promote cell alignment with the guidance of the aligned microfibers produced via melt electrospinning writing in hierarchical scaffolds.
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http://dx.doi.org/10.1088/1748-605X/ac0a90DOI Listing
June 2021

Mechanical property characterization of partially crystalline Poly-Ether-Ether-Ketone.

J Mech Behav Biomed Mater 2021 Jun 1;121:104600. Epub 2021 Jun 1.

CAS Key Laboratory for Mechanical Behavior and Design of Materials, Department of Modern Mechanics. University of Science and Technology of China, Hefei, Anhui, 230027, China.

The present study investigates the mechanical properties of partially crystalline Poly-Ether-Ether-Ketone between the glass transition and the cold crystallization. Biaxial tension, uniaxial tension, and DMA experiments were conducted to investigate the influence of temperature-induced crystallization on mechanical properties, and three stiffening behaviors are observed. Firstly, a 'U' type mechanical property is observed for all three experiments with first softening and then significant stiffening behavior with increasing temperature. Secondly, stiffening also occurs during low strain rate tests but not in higher strain rate tests. Thirdly, the stiffening behavior of the anisotropic film shows orientation dependence. Crystallinity evolution is predicted by the Nakaruma non-isothermal crystallization kinetics with optimized parameters, with which we demonstrate and explain that the stiffening behaviors are connected to the onset of crystallization. Therefore, the conclusion provides a new tool to approach and distinguish extrinsic and intrinsic properties during characterization, promoting future implementation for constitutive modeling and corresponding simulation that could replicate the influence of temperature-induced crystallization.
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http://dx.doi.org/10.1016/j.jmbbm.2021.104600DOI Listing
June 2021

LncRNA CCDC144NL-AS1 Serves as a Prognosis Biomarker for Non-small Cell Lung Cancer and Promotes Cellular Function by Targeting miR-490-3p.

Mol Biotechnol 2021 Jun 11. Epub 2021 Jun 11.

Department of Thoracic Surgery, The Second Hospital of Jilin University, 218 Ziqiang Street, Changchun, 130041, Jilin, China.

This study revealed the prognostic significance of long non-coding RNA (lncRNA) CCDC144NL-AS1 in NSCLC patients and discussed the effect and mechanism of proliferation, migration, and invasion of non-small cell lung cancer (NSCLC) cells. 128 pairs of NSCLC tissues and paracancerous tissues were collected, and qRT-PCR was used to detect the differential expression of lncRNA CCDC144NL-AS1 in all tissues and cells lines. Kaplan-Meier analysis and Cox proportional hazards model analysis were used to estimate the prognostic value of lncRNA CCDC144NL-AS1. CCK-8 and Transwell assays confirmed the effect of lncRNA CCDC144NL-AS1 on the proliferation, migration, and invasion of NSCLC. Bioinformatics was used to predict the microRNAs that lncRNA CCDC144NL-AS1 might bind to miR-490-3p. The regulation of lncRNA CCDC144NL-AS1 on miR-490-3p was verified by luciferase activity assay with wide type or mutation. The expression of lncRNA CCDC144NL-AS1 was enhanced in both NSCLC tissues and cell lines. Patients with overexpression of lncRNA CCDC144NL-AS1 have a poor prognosis, and lncRNA CCDC144NL-AS1 is an independent prognostic factor for NSCLC. Increased the relative expression level of lncRNA CCDC144NL-AS1 can promote the proliferation, migration, and invasion of NSCLC cells. LncRNA CCDC144NL-AS1 might target miR-490-3p. LncRNA CCDC144NL-AS1 can be used as an oncogene of NSCLC to predict patient prognosis and promote tumor proliferation, migration, and invasion by targeting miR-490-3p.
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http://dx.doi.org/10.1007/s12033-021-00351-6DOI Listing
June 2021

CIRBP promotes ferroptosis by interacting with ELAVL1 and activating ferritinophagy during renal ischaemia-reperfusion injury.

J Cell Mol Med 2021 Jun 10. Epub 2021 Jun 10.

Department of Organ Transplantation, Shanghai Changhai Hospital, Shanghai, China.

Renal ischaemia-reperfusion (IR) is a major cause of acute kidney injury (AKI). Cold-inducible RNA-binding protein (CIRBP) may contribute to AKI because its deficiency protects against renal IR injury in a mechanism believed to involve ferroptosis. We aimed to investigate whether ferroptosis is associated with CIRBP-mediated renal damage. The differential expression of CIRBP was examined in tubular epithelial (HK2) cells during hypoxia-reoxygenation (HR) or in response to erastin, an inducer of ferroptosis. CIRBP expression was increased in response to HR or erastin in HK2 cells but the silencing of CIRBP inhibited HR and erastin-induced ferroptosis together with ferritinophagy. We discovered an interaction between CIRBP and ELAVL1 using STRING software, which was verified through co-immunoprecipitation and fluorescence colocalization assays. We found that ELAVL1 is a critical regulator in the activation of ferritinophagy and the promotion of ferroptosis. HR or erastin also induced the expression of ELAVL1. An autophagy inhibitor (hydroxychloroquine) or si-ELAVL1 transfection reversed CIRBP-enhanced ferritinophagy activation and ferroptosis in HK2 cells under HR. Injection of anti-CIRBP antibody into a mouse model of IR inhibited ferroptosis and decreased renal IR injury in vivo. In summary, our results provide evidence that ferritinophagy-mediated ferroptosis could be responsible for CIRBP-enhanced renal IR injury.
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http://dx.doi.org/10.1111/jcmm.16567DOI Listing
June 2021

Room-Temperature Magnetic Field Effect on Excitonic Photoluminescence in Perovskite Nanocrystals.

Adv Mater 2021 Jun 10:e2008225. Epub 2021 Jun 10.

State Key Laboratory of Materials Processing and Die & Mould Technology, School of Materials Science and Engineering, Huazhong University of Science and Technology, Wuhan, Hubei, 430074, P. R. China.

Magnetic-field-enhanced spin-polarized electronic/optical properties in semiconductors are crucial for fabricating various spintronic devices. However, this spin polarization is governed by weak spin exchange interactions and easily randomized by thermal fluctuations; therefore, it is only produced at cryogenic temperatures, which severely limits the applications. Herein, a room-temperature intrinsic magnetic field effect (MFE) on excitonic photoluminescence is achieved in CsPbX :Mn (X = Cl, Br) perovskite nanocrystals. Through moderate Mn doping, the MFE is enhanced by exciton-Mn interactions, and through partial Br substitution, the MFE is stabilized at room temperature by exciton orbital ordering. The orbital ordering significantly enhances the g-factor difference between electrons and holes, which is evidenced by a parallel orbit-orbit interaction among excitons generated by circular polarized laser excitation. This study provides a clear avenue for engineering spintronic materials based on orbital interactions in perovskites.
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http://dx.doi.org/10.1002/adma.202008225DOI Listing
June 2021

Children ASD Evaluation Through Joint Analysis of EEG and Eye-Tracking Recordings With Graph Convolution Network.

Front Hum Neurosci 2021 25;15:651349. Epub 2021 May 25.

School of Computer Science, Wuhan University, Wuhan, China.

Recent advances in neuroscience indicate that analysis of bio-signals such as rest state electroencephalogram (EEG) and eye-tracking data can provide more reliable evaluation of children autism spectrum disorder (ASD) than traditional methods of behavior measurement relying on scales do. However, the effectiveness of the new approaches still lags behind the increasing requirement in clinical or educational practices as the "bio-marker" information carried by the bio-signal of a single-modality is likely insufficient or distorted. This study proposes an approach to joint analysis of EEG and eye-tracking for children ASD evaluation. The approach focuses on deep fusion of the features in two modalities as no explicit correlations between the original bio-signals are available, which also limits the performance of existing methods along this direction. First, the synchronization measures, information entropy, and time-frequency features of the multi-channel EEG are derived. Then a random forest applies to the eye-tracking recordings of the same subjects to single out the most significant features. A graph convolutional network (GCN) model then naturally fuses the two group of features to differentiate the children with ASD from the typically developed (TD) subjects. Experiments have been carried out on the two types of the bio-signals collected from 42 children (21 ASD and 21 TD subjects, 3-6 years old). The results indicate that (1) the proposed approach can achieve an accuracy of 95% in ASD detection, and (2) strong correlations exist between the two bio-signals collected even asynchronously, in particular the EEG synchronization against the face related/joint attentions in terms of covariance.
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http://dx.doi.org/10.3389/fnhum.2021.651349DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185139PMC
May 2021