Publications by authors named "Zhandong Bo"

8 Publications

  • Page 1 of 1

Risk score model of autophagy-related genes in osteosarcoma.

Ann Transl Med 2022 Mar;10(5):252

Department of Bone and Joint Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, China.

Background: Osteosarcoma (OS) is a common pediatric malignancy with high mortality and disability rates. Autophagy is an essential process in regulating the apoptosis and invasion of tumor cells, so constructing a risk score model of OS autophagy-related genes (ARGs) will bring benefit to the evaluation of both treatment and prognosis.

Methods: We downloaded a dataset of OS from the Therapeutically Applicable Research To Generate Effective Treatments (TARGET) database, and found OS-related ARGs through the Human Autophagy Database (HADb). Five hub ARGs (, , , and ) were obtained using a multivariate Cox regression model. We then generated the risk scores and constructed a prediction model. Another dataset obtained from the Gene Expression Omnibus (GEO) was used to test accuracy and validity. The role of immune cell infiltration was systematically explored, and prediction of response to targeted drugs was assessed. Immunohistochemistry was carried out to verify the expression of the key ARGs.

Results: Based on the five hub ARGs, we established a risk score model related to OS. High accuracy and validity were demonstrated by datasets downloaded from the GEO. The five ARGs played a role in the PI3K and MAPK pathways. Results from targeted drug sensitivity analyses were consistent with pathway analyses. Immunohistochemistry demonstrated that the expression differences of the five ARGs were significant between the OS group and the paracancerous group.

Conclusions: We constructed a risk score model related to autophagy of OS, explored the diagnostic value of ARGs, and present possible therapeutic targets.
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http://dx.doi.org/10.21037/atm-22-166DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8987880PMC
March 2022

A Risk-Scoring Model Based on Evaluation of Ferroptosis-Related Genes in Osteosarcoma.

J Oncol 2022 28;2022:4221756. Epub 2022 Mar 28.

Department of Bone and Joint Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China.

Background: Osteosarcoma (OS) is a bone malignancy frequently seen in pediatrics and has high mortality and incidence. Ferroptosis is an important cell death process in regulating the apoptosis and invasion of tumor cells, so constructing the risk-scoring model based on OS ferroptosis-related genes (FRGs) will benefit the evaluation of both treatment and prognosis.

Methods: The OS dataset was screened from the Therapeutically Applicable Research to Generate Effective Treatments (TARGET) database, and OS-related FRGs were found through the Ferroptosis Database (FerrDb) using a multivariate Cox regression model, followed by the generation of the risk scores and a risk-scoring prediction model. Further systematical exploration for immune cell infiltration and assessing the prediction of response to targeted drugs was conducted.

Results: Based on OS-related FRGs, a risk-scoring model of FRGs in OS was constructed. The six FRGs played a role in the carbon metabolism, glutathione metabolism, and pentose phosphate pathways. Results from targeted drug sensitivity analyses were concordant to pathway analyses. The response to targeted drugs statistically differed between the two groups with different risks, and the high-risk group presented a high sensitivity to targeted drugs.

Conclusions: We identified a 6-ferroptosis-gene-based prognostic signature in OS and created and verified a risk-scoring model to predict the prognosis of OS at 1, 3, and 5 years for OS patients independently.
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http://dx.doi.org/10.1155/2022/4221756DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8979715PMC
March 2022

The efficacy and safety of fast track surgery (FTS) in patients after hip fracture surgery: a meta-analysis.

J Orthop Surg Res 2021 Feb 27;16(1):162. Epub 2021 Feb 27.

Department of Bone and Joint Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.

Background: Fast track surgery (FTS) has been gradually applied in perioperative management of orthopedic surgery, but there still some research suspected that the prognosis of patients is not as expected and the cost is high, the effect of the FTS still urgently needed for support by evidence-based medicine.

Methods: We retrieved RCTs from medical research literature databases. Risk ratios (RR), standard mean difference (SMD), and 95% confidence intervals (CI) were calculated to compare the primary and safety endpoints.

Results: Overall, a total of 8886 patients were retrieved from 57 articles, of which 4448 patients (50.06%) were randomized to experimental group whereas 4438 patients (49.94%) were randomized to control group. The result showed that FTS could significantly shorten the length of stay (LOS), decrease the visual analog scale (VAS), reduce the leaving bed time and the hospitalization costs, and improve Harris hip joint function score. The incidence of complications such as respiratory system infection, urinary system infection, venous thrombus embolism (VTE), pressure sore, incision infection, constipation, and prosthesis dislocation also has been decreased significantly. Meanwhile, FTS improved patients' satisfaction apparently.

Conclusions: This meta-analysis reveals that FTS could significantly shorten the length of stay, alleviate the pain, reduce the leaving bed time and the hospitalization costs, and improve hip function. The incidence of complications also has been decreased significantly. Meanwhile, FTS has been spoken highly in patients in terms of nursing satisfaction. Its efficacy and safety were proved to be reliable.
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http://dx.doi.org/10.1186/s13018-021-02277-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7913454PMC
February 2021

The efficacy and safety of selective COX-2 inhibitors for postoperative pain management in patients after total knee/hip arthroplasty: a meta-analysis.

J Orthop Surg Res 2020 Feb 5;15(1):39. Epub 2020 Feb 5.

Department of Bone and Joint Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.

Background: Many selective cyclooxygenase (COX-2) inhibitors are currently used in clinical practice. COX-2 inhibitors have good anti-inflammatory, analgesic, antipyretic effects, and gastrointestinal safety. However, the analgesic effects and adverse reactions of COX-2 after total knee/hip arthroplasty (TKA/THA) are not fully known.

Objective: To evaluate the efficacy and safety of selective COX-2 inhibitors in postoperative pain management in patients receiving TKA/THA.

Methods: Randomized controlled trials (RCTs) were retrieved from medical literature databases. Risk ratios (RR) Std mean difference (SMD) and 95% confidence intervals (CI) were calculated to analyze the primary and safety endpoints.

Results: In total, 18 articles (23 trial comparisons) were retrieved comprising 3104 patients. Among them, 1910 patients (61.5%) were randomized to the experimental group whereas 1194 patients (38.5%) were randomized to the control group. The primary endpoints were the patients' VAS score at rest or on ambulation (within 3 days). We found that VAS score in patients that received selective COX-2 inhibitor was significantly lower compared to those of the control group.

Conclusion: This meta-analysis shows that selective COX-2 inhibitor therapy is effective, safe, and reliable in relieving postoperative pain of THA/TKA.
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http://dx.doi.org/10.1186/s13018-020-1569-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7003344PMC
February 2020

Impaired osteogenic differentiation associated with connexin43/microRNA-206 in steroid-induced avascular necrosis of the femoral head.

Exp Mol Pathol 2016 08 21;101(1):89-99. Epub 2016 Jul 21.

Department of Bone and Joint Surgery, the First Affiliated Hospital, Guangxi Medical University, Nanning, China.

Connexin(Cx)43 and microRNA(miR)-206 play an important role in osteogenesis. However, their role in steroid-induced femoral head osteonecrosis (SANFH) is still ambiguous. The present study aimed to establish a rabbit model and investigate osteogenesis in steroid-induced femoral head osteonecrosis occurring via Cx43/miR-206 and the changes of Wnt/β-catenin signal pathway-related proteins. A total of 72 adult New Zealand white rabbits were divided randomly into a model group (Group A) and a control group (Group B) of 36 rabbits each. Group A was injected intravenously with lipopolysaccharide (10μg/kg body weight, once per day). After 48h, three injections of methylprednisolone (MPS; 20mg/kg body weight) were administered intramuscularly at 24-hour intervals. Group B were fed and housed under identical conditions but received saline injections. All animals were sacrificed at two, four, and eight weeks from the first MPS injection. Typical early osteonecrosis symptoms were observed in Group A. The expression of miR-206 in Group A was significantly higher than that of Group B. The mRNA and protein levels of Cx43, β-catenin, runt-related transcription factor 2, and alkaline phosphatase gradually decreased while Dickkopf-1 (Dkk-1) gradually increased in Group A compared with Group B. These findings indicated that Cx43/miR-206 is involved in the pathogenesis of early stage SANFH and may be associate with Wnt/β-catenin signal pathway.
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http://dx.doi.org/10.1016/j.yexmp.2016.07.009DOI Listing
August 2016

[Progress of peripheral nerve defect treatment with tissue engineering].

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2010 Nov;24(11):1310-4

Department of Orthopaedics and Traumatology, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, P R China.

Objective: To review new progress of related research of peripheral nerve defect treatment with tissue engineering in recent years.

Methods: Domestic and international literature concerning peripheral nerve defect treatment with tissue engineering was reviewed and analyzed.

Results: Releasing neurotrophic factors with sustained release technology included molecular biology techniques, poly (lactic-co-glycolic acid) microspheres, and polyphosphate microspheres. The mixture of neurotrophic factors and ductus was implanted to the neural tube wall which could be degraded then releasing factors slowly. Seed cells which were the major source of active ingredients played an important role in the repair and reconstruction of tissue engineering products. The neural tube of Schwann cells made long nerve repair and the quality of nerve regeneration was improved. Nerve scaffold materials included natural and synthetic biodegradable materials. Tube structure usually was adopted for nerve scaffold, which performance would affect the nerve repair effects directly.

Conclusion: With the further research of tissue engineering, the treatment of peripheral nerve defects with tissue engineering has made significant progress.
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November 2010

A meta-analysis of hamstring autografts versus bone-patellar tendon-bone autografts for reconstruction of the anterior cruciate ligament.

Knee 2011 Oct 17;18(5):287-93. Epub 2010 Sep 17.

Department of Orthopaedics Trauma and Hand Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning 530027, China.

The objective of this study was to evaluate the effectiveness of hamstring (HT) autografts versus bone-patellar tendon-bone (BPTB) autografts for reconstruction of the anterior cruciate ligament (ACL). We searched the Cochrane Library, MEDLINE, EMBASE and the Chinese Biomedicine Database (CBM) for published randomised clinical trials (RCTs) relevant to ACL reconstruction comparing HT and BPTB autografts. Data analyses were performed with Cochrane Collaboration's RevMan 5.0. A total of 23 reports of 19 randomised controlled trials (RCTs) (1643 patients) met the inclusion criteria. Outcomes favouring BPTB autografts were found in terms of KT-1000 arithmometer values, negative rates of Lachman tests and negative rates of Pivot tests. Outcome measures that favoured HT autografts included anterior knee pain, kneeling pain and extension loss. There was no statistical difference of postoperative graft failure. Overall, postoperative complications of the knee joint were lower for HT autografts than for BPTB autografts, and BPTB autografts were superior to HT autografts in resuming stability of the knee joint, but four-strand HT combined with application of the modern endobutton HT graft-fixation technique could increase knee-joint stability.
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http://dx.doi.org/10.1016/j.knee.2010.08.002DOI Listing
October 2011

[Study on effect of NGF on fracture healing].

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2009 May;23(5):570-6

Department of Orthopedics, the First Affiliated Hospital of Guangxi Medical University, Nanning Guangxi, 530021, P.R. China.

Objective: To investigate the effect of NGF on fracture healing, and to study the role of BMP-2 induced osteoblast.

Methods: Sixty cleaned male Kunming mice (aging 6-8 weeks and weighing 23-25 g) were made fracture models in the middle of femoral shaft and randomly divided into four groups (groups A, B, C and D, n=15). Fracture was treated with NGF/normal saline, BMP-2, BMP-2/NGF/normal saline, and normal saline in groups A, B, C and D, respectively. After 14, 21 and 28 days, the specimens were selected from 5 mice each group to do the biochemical and histological analysis. Before the mice were killed, the arteriovenous blood was taken from their eye-ball to test the ALP activity.

Results: After 14 days, 21 days and 28 days, the gross observation showed that the size and hardness of bone tissue, and callus tissue growth increased in groups A, B and C order and were higher than those in group D; the X-ray films showed that the calcified area increased in groups A, B and C order and were higher than those in group D; the histological observation showed that the trabecular maturity increased in groups A, B and C order and were higher than those in group D. The osteoblast area, the gray degree value of the radiographs in callus tissue, the ALP contents of serum and callus tissue, calcium content of callus tissue and net weight of callus were higher in groups A, B and C than in group D. There were significant differences (P < 0.05) in osteoblast area and gray degree values of the radiographs at 14, 21 and 28 days; in ALP contents of serum at 14 days; in ALP contents of callus tissue at 14 days and 21 days; in calcium content of callus tissue at 21 days and 28 days among 4 groups. There were significant differences in net weight of callus between groups B, C and groups A, D at 14 days (P < 0.05). At 21 days and 28 days, the trabecular surface index of osteoblast, the average trabecular volume and the mean trabecular width decreased as time went on, having an increase order of groups A, B, C and was higher in groups A, B, C than in group D, showing significant differences among 4 groups (P < 0.05).

Conclusion: NGF promotes the healing of fractures. NGF possesses synergistic effect on ectopic bone formation induced by BMP-2.
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May 2009
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