Publications by authors named "Zenghui Hu"

13 Publications

  • Page 1 of 1

Event-Triggered Impulsive Control for Nonlinear Stochastic Systems.

IEEE Trans Cybern 2021 Feb 10;PP. Epub 2021 Feb 10.

We study the stabilization problem for nonlinear stochastic systems via an event-triggered impulsive control (ETIC) scheme, where the impulsive control time sequence is generated by the event-triggered mechanism (ETM). Both continuous ETM and periodic ETM are developed by continuous measuring and periodic sampling, respectively. The continuous ETM with time regularization is proposed to exclude the Zeno behavior. The upper bound of the sampling period is given for the periodic ETM. By means of the continuous ETM and periodic ETM, sufficient conditions are given to guarantee the pth moment uniform stability and the pth moment exponential stability of related systems. Moreover, LMI-based conditions of exponential stability in the mean square are established for linear stochastic systems under ETIC. Finally, two examples are presented to illustrate the proposed ETIC schemes, in which an example of the consensus of linear stochastic multiagent systems is considered.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1109/TCYB.2021.3052166DOI Listing
February 2021

Development of EST-SSR markers and association mapping with floral traits in Syringa oblata.

BMC Plant Biol 2020 Sep 21;20(1):436. Epub 2020 Sep 21.

Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing University of Agriculture, Beijing, 102206, China.

Background: Lilac (Syringa oblata) is an important woody plant with high ornamental value. However, very limited genetic marker resources are currently available, and little is known about the genetic architecture of important ornamental traits for S. oblata, which is hindering its genetic studies. Therefore, it is of great significance to develop effective molecular markers and understand the genetic architecture of complex floral traits for the genetic research of S. oblata.

Results: In this study, a total of 10,988 SSRs were obtained from 9864 unigene sequences with an average of one SSR per 8.13 kb, of which di-nucleotide repeats were the dominant type (32.86%, 3611). A set of 2042 primer pairs were validated, out of which 932 (45.7%) exhibited successful amplifications, and 248 (12.1%) were polymorphic in eight S. oblata individuals. In addition, 30 polymorphic EST-SSR markers were further used to assess the genetic diversity and the population structure of 192 cultivated S. oblata individuals. Two hundred thirty-four alleles were detected, and the PIC values ranged from 0.23 to 0.88 with an average of 0.51, indicating a high level of genetic diversity within this cultivated population. The analysis of population structure showed two major subgroups in the association population. Finally, 20 significant associations were identified involving 17 markers with nine floral traits using the mixed linear model. Moreover, marker SO104, SO695 and SO790 had significant relationship with more than one trait.

Conclusion: The results showed newly developed markers were valuable resource and provided powerful tools for genetic breeding of lilac. Beyond that, our study could serve an efficient foundation for further facilitate genetic improvement of floral traits for lilac.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12870-020-02652-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7507607PMC
September 2020

Nitric oxide modulating ion balance in Hylotelephium erythrostictum roots subjected to NaCl stress based on the analysis of transcriptome, fluorescence, and ion fluxes.

Sci Rep 2019 12 4;9(1):18317. Epub 2019 Dec 4.

Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing University of Agriculture, Beijing, 102206, China.

Soil salinization is one of the main stress factors that affect both growth and development of plants. Hylotelephium erythrostictum exhibits strong resistance to salt, but the underlying genetic mechanisms remain unclear. In this study, hydroponically cultured seedlings of H. erythrostictum were exposed to 200 mM NaCl. RNA-Seq was used to determine root transcriptomes at 0, 5, and 10 days, and potential candidate genes with differential expression were analyzed. Transcriptome sequencing generated 89.413 Gb of raw data, which were assembled into 111,341 unigenes, 82,081 of which were annotated. Differentially expressed genes associated to Na and K transport, Ca channel, calcium binding protein, and nitric oxide (NO) biosynthesis had high expression levels in response to salt stress. An increased fluorescence intensity of NO indicated that it played an important role in the regulation of the cytosolic K/Na balance in response to salt stress. Exogenous NO donor and NO biosynthesis inhibitors significantly increased and decreased the Na efflux, respectively, thus causing the opposite effect for K efflux. Moreover, under salt stress, exogenous NO donors and NO biosynthesis inhibitors enhanced and reduced Ca influx, respectively. Combined with Ca reagent regulation of Na and K fluxes, this study identifies how NaCl-induced NO may function as a signaling messenger that modulates the K/Na balance in the cytoplasm via the Ca signaling pathway. This enhances the salt resistance in H. erythrostictum roots.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-019-54611-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6892800PMC
December 2019

Evaluation of the environmental and plant growth effectiveness of a new substrate consisting of municipal sludge and fly ash.

Waste Manag 2019 Nov 5;99:163-171. Epub 2019 Sep 5.

Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing University of Agriculture, Beijing 102206, PR China; Collaborative Innovation Center for Eco-environmental Improvement with Forestry and Fruit Trees, Beijing University of Agriculture, Beijing 102206, PR China; College of Landscape Architecture, Beijing University of Agriculture, Beijing 102206, PR China. Electronic address:

In order to seek a safe, sustainable, and low-cost method for reuse of municipal sewage sludge, four species of native plants, i.e., Forsythia suspensa, Sophora japonica, Cotinus coggygria, and Ailanthus altissima were planted in flowerpots containing 4 growth substrates consisting of raw sludge and fly ash at volume/volume ratios of 20:80, 40:60, 60:40, and 80:20, respectively. The results showed that the physiochemical characteristics of the sewage sludge and fly ash were complementary. The sludge supplied the nutrients and the fly ash maintained air permeability in the mixed substrate. The mixed substrates containing 40-60% sewage sludge that belonged to sand clay loam were suitable for the seedling growth of the four species. After the end of the growing season, the electrical conductivity, pH, and contents of organic matter, nitrogen, potassium, and heavy metals in the four growth substrates decreased significantly. Moreover, most of the heavy metals were removed from the substrates by seedling root system. A. altissima grew best, and heavy metal enrichments of F. suspense and C. coggygria were stronger than other two species. The results indicate that the new substrates containing 40-60% sludge exhibiting good physiochemical properties, are environmentally friendly, and suitable for landscape planting.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.wasman.2019.08.033DOI Listing
November 2019

Feasible and effective reuse of municipal sludge for vegetation restoration: physiochemical characteristics and microbial diversity.

Sci Rep 2019 01 29;9(1):879. Epub 2019 Jan 29.

Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing University of Agriculture, Beijing, 102206, P.R. China.

The large volume of municipal sludge causes environmental problems in cities worldwide. In this study, municipal sludge, mixed with construction waste residue, was used as substrate to plant Ailanthus altissima. The growth of A. altissima, the substrate characteristics, and substrate microbial diversity were measured to investigate potential recycling and reusing pathways of municipal sludge. The obtained results showed that compared to garden soil, the mixed substrate was weakly alkaline, and had higher nutrient contents, which is beneficial for A. altissima, and results in better growth in mixed substrate. Although the contents of the main heavy metals in the mixed substrate were significantly higher than in garden soil, the values met the criterion of Class II soil in the Environmental Quality Standard for the Soils of China (GB15618-1995). Different substrates showed a variety of microbial diversities. Proteobacteria was the top microbial phylum in all samples, and higher relative abundances were found in samples containing municipal sludge. After growing A. altissima, the relative abundances of Acidobacteria and Gemmatimonadetes increased in the mixed substrate. Therefore, addition of construction waste residue and growth of A. altissima caused a difference. The microbial communities in the mixed substrate with A. altissima are both plant friendly and environmentally friendly. These results suggest this mixed substrate as a potentially feasible and effective pathway for the reuse and recycling of municipal sludge for vegetation restoration.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-018-37338-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351607PMC
January 2019

Interaction of ciprofloxacin with the activated sludge of the sewage treatment plant.

Environ Sci Pollut Res Int 2018 Dec 16;25(35):35064-35073. Epub 2018 Oct 16.

Faculty of Architectural, Civil Engineering and Environment, Ningbo University, Ningbo, 315211, China.

Interaction of ciprofloxacin with the activated sludge of the sewage treatment plant is of importance for the ciprofloxacin migration and risk control. More than 96.0% ciprofloxacin was removed through the sludge adsorption. The sludge surface charge varied little with ciprofloxacin since most ciprofloxacin was dissociated into the neutral one. No obvious shift was observed for the soluble carbohydrate concentration and composition with the addition of ciprofloxacin, indicating the weak interaction between the carbohydrates and ciprofloxacin. The introduction of ciprofloxacin resulted in a reduction of the soluble protein concentration, a marked increase of the extracellular protein fluorescence intensities, and a dramatic emergence of new extracellular proteins. The alteration of the proteins highlights the strong interaction between the extracellular proteins and ciprofloxacin, and the consequent integration of certain soluble proteins and original unextractable inner layer extracellular proteins into the extractable extracellular proteins. Different types of interactions are suggested to dominate between the extracellular proteins and the differently dissociated ciprofloxacin.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11356-018-3413-0DOI Listing
December 2018

Antioxidant effect of aromatic volatiles emitted by Lavandula dentata, Mentha spicata, and M. piperita on mouse subjected to low oxygen condition.

Biosci Biotechnol Biochem 2017 Dec 13;81(12):2386-2395. Epub 2017 Oct 13.

a College of Landscape Architecture , Beijing University of Agriculture , Beijing , China.

This study aims to investigate the antioxidant effect of aromatic volatiles of three common aromatic plants, Lavandula dentata, Mentha spicata, and M. piperita. In this study, kunming mice subjected to low oxygen condition were treated with the volatiles emitted from these aromatic plants through inhalation administration. Then the blood cell counts, and the activities and gene expressions of antioxidant enzymes in different tissues were tested. The results showed that low oxygen increased the counts of red blood cells, white blood cells, and blood platelets of mice, and aromatic volatiles decreased their counts. Exposure to aromatic volatiles resulted in decreases in the malonaldehyde contents, and increases in the activities and gene expressions of superoxide dismutase, glutathione peroxidase, and catalase in different tissues under low oxygen. In addition, as the main component of aromatic volatiles, eucalyptol was the potential source that imparted positive antioxidant effect.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/09168451.2017.1385382DOI Listing
December 2017

Molecular characterization and functional analysis of chalcone synthase from Syringa oblata Lindl. in the flavonoid biosynthetic pathway.

Gene 2017 Nov 7;635:16-23. Epub 2017 Sep 7.

College of Landscape Architecture, Beijing University of Agriculture, Beijing 102206, China; Beijing Collaborative Innovation Center for Eco-Environmental Improvement with Forestry and Fruit Trees, Beijing 102206, China. Electronic address:

The flower color of Syringa oblata Lindl., which is often modulated by the flavonoid content, varies and is an important ornamental feature. Chalcone synthase (CHS) catalyzes the first key step in the flavonoid biosynthetic pathway. However, little is known about the role of S. oblata CHS (SoCHS) in flavonoid biosynthesis in this species. Here, we isolate and analyze the cDNA (SoCHS1) that encodes CHS in S. oblata. We also sought to analyzed the molecular characteristics and function of flavonoid metabolism by SoCHS1. We successfully isolated the CHS-encoding genomic DNA (gDNA) in S. oblata (SoCHS1), and the gene structural analysis indicated it had no intron. The opening reading frame (ORF) sequence of SoCHS1 was 1170bp long and encoded a 389-amino acid polypeptide. Multiple sequence alignment revealed that both the conserved CHS active site residues and CHS signature sequence were in the deduced amino acid sequence of SoCHS1. Crystallographic analysis revealed that the protein structure of SoCHS1 is highly similar to that of FnCHS1 in Freesia hybrida. The quantitative real-time polymerase chain reaction (PCR) performed to detect the SoCHS1 transcript expression levels in flowers, and other tissues revealed the expression was significantly correlated with anthocyanin accumulation during flower development. The ectopic expression results of Nicotiana tabacum showed that SoCHS1 overexpression in transgenic tobacco changed the flower color from pale pink to pink. In conclusion, these results suggest that SoCHS1 plays an essential role in flavonoid biosynthesis in S. oblata, and could be used to modify flavonoid components in other plant species.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.gene.2017.09.002DOI Listing
November 2017

Proteomic analysis of Potentilla fruticosa L. leaves by iTRAQ reveals responses to heat stress.

PLoS One 2017 22;12(8):e0182917. Epub 2017 Aug 22.

Shandong Forest Germplasm Resources Center, Jinan City, Shandong Province, China.

High temperature is an important environmental factor that affects plant growth and crop yield. Potentilla fruticosa L. has a developed root system and characteristics of resistance to several stresses (e.g., high temperature, cold, drought) that are shared by native shrubs in the north and west of China. To investigate thermotolerance mechanisms in P. fruticosa, 3-year-old plants were subjected to a high temperature of 42°C for 1, 2, and 3 days respectively before analysis. Then, we studied changes in cell ultrastructure using electron microscopy and investigated physiological changes in the leaves of P. fruticosa. Additionally, we used isobaric tags for relative and absolute quantification (iTRAQ) coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) to study proteomic changes in P. fruticosa leaves after 3 d of 42°C heat stress. we found that the cell membrane and structure of chloroplasts, especially the thylakoids in P. fruticosa leaves, was destroyed by a high temperature stress, which might affect the photosynthesis in this species. We identified 35 up-regulated and 23 down-regulated proteins after the heat treatment. Gene Ontology (GO) analysis indicated that these 58 differentially abundant proteins were involved mainly in protein synthesis, protein folding and degradation, abiotic stress defense, photosynthesis, RNA process, signal transduction, and other functions. The 58 proteins fell into different categories based on their subcellular localization mainly in the chloroplast envelope, cytoplasm, nucleus, cytosol, chloroplast, mitochondrion and cell membrane. Five proteins were selected for analysis at the mRNA level; this analysis showed that gene transcription levels were not completely consistent with protein abundance. These results provide valuable information for Potentilla thermotolerance breeding.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0182917PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5568749PMC
October 2017

Transcriptome Sequencing Analysis Reveals a Difference in Monoterpene Biosynthesis between Scented 'Siberia' and Unscented 'Novano'.

Front Plant Sci 2017 4;8:1351. Epub 2017 Aug 4.

College of Landscape Architecture, Beijing University of AgricultureBeijing, China.

is a world famous fragrant bulb flower with high ornamental and economic values, and significant differences in fragrance are found among different genotypes. In order to explore the mechanism underlying the different fragrances, the floral scents of 'Sibeia', with a strong fragrance, and 'Novano', with a very faint fragrance, were collected using a dynamic headspace technique. These scents were identified using automated thermal desorption-gas chromatography/mass spectrometry (ATD-GC/MS) at different flowering stages. We used RNA-Seq technique to determine the petal transcriptome at the full-bloom stage and analyzed differentially expressed genes (DEGs) to investigate the molecular mechanism of floral scent biosynthesis. The results showed that a significantly higher amount of 'Siberia' floral scent was released compared with 'Novano'. Moreover, monoterpenes played a dominant role in the floral scent of 'Siberia'; therefore, it is believed that the different emissions of monoterpenes mainly contributed to the difference in the floral scent between the two genotypes. Transcriptome sequencing analysis indicated that ~29.24 Gb of raw data were generated and assembled into 124,233 unigenes, of which 35,749 unigenes were annotated. Through a comparison of gene expression between these two genotypes, 6,496 DEGs were identified. The genes in the terpenoid backbone biosynthesis pathway showed significantly different expression levels. The gene expressions of 1-deoxy-D-xylulose 5-phosphate synthase (DXS), 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR), 4-hydroxy-3-methylbut-2-enyl diphosphate synthase (HDS), 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (HDR), isopentenyl diphosphate isomerase (IDI), and geranyl diphosphate synthase (GPS/GGPS), were upregulated in 'Siberia' compared to 'Novano', and two monoterpene synthase genes, ocimene synthase gene () and myrcene synthase gene (), were also expressed at higher levels in the tepals of 'Siberia', which was consistent with the monoterpene release amounts. We demonstrated that the high activation levels of the pathways contributed to monoterpene biosynthesis in 'Siberia' resulting in high accumulations and emissions of monoterpenes, which led to the difference in fragrance between these two genotypes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpls.2017.01351DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5543080PMC
August 2017

De novo transcriptomic analysis and development of EST-SSRs for Sorbus pohuashanensis (Hance) Hedl.

PLoS One 2017 14;12(6):e0179219. Epub 2017 Jun 14.

College of Landscape Architecture and Forestry, Qingdao Agricultural University, Qingdao, China.

Sorbus pohuashanensis is a native tree species of northern China that is used for a variety of ecological purposes. The species is often grown as an ornamental landscape tree because of its beautiful form, silver flowers in early summer, attractive pinnate leaves in summer, and red leaves and fruits in autumn. However, development and further utilization of the species are hindered by the lack of comprehensive genetic information, which impedes research into its genetics and molecular biology. Recent advances in de novo transcriptome sequencing (RNA-seq) technology have provided an effective means to obtain genomic information from non-model species. Here, we applied RNA-seq for sequencing S. pohuashanensis leaves and obtained a total of 137,506 clean reads. After assembly, 96,213 unigenes with an average length of 770 bp were obtained. We found that 64.5% of the unigenes could be annotated using bioinformatics tools to analyze gene function and alignment with the NCBI database. Overall, 59,089 unigenes were annotated using the Nr database(non-redundant protein database), 35,225 unigenes were annotated using the GO (Gene Ontology categories) database, and 33,168 unigenes were annotated using COG (Cluster of Orthologous Groups). Analysis of the unigenes using the KEGG (Kyoto Encyclopedia of Genes and Genomes) database indicated that 13,953 unigenes were involved in 322 metabolic pathways. Finally, simple sequence repeat (SSR) site detection identified 6,604 unigenes that included EST-SSRs and a total of 7,473 EST-SSRs in the unigene sequences. Fifteen polymorphic SSRs were screened and found to be of use for future genetic research. These unigene sequences will provide important genetic resources for genetic improvement and investigation of biochemical processes in S. pohuashanensis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0179219PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5470691PMC
September 2017

Transcriptome Analysis of Syringa oblata Lindl. Inflorescence Identifies Genes Associated with Pigment Biosynthesis and Scent Metabolism.

PLoS One 2015 20;10(11):e0142542. Epub 2015 Nov 20.

College of Landscape Architecture, Beijing University of Agriculture, Beijing, 102206, China.

Syringa oblata Lindl. is a woody ornamental plant with high economic value and characteristics that include early flowering, multiple flower colors, and strong fragrance. Despite a long history of cultivation, the genetics and molecular biology of S. oblata are poorly understood. Transcriptome and expression profiling data are needed to identify genes and to better understand the biological mechanisms of floral pigments and scents in this species. Nine cDNA libraries were obtained from three replicates of three developmental stages: inflorescence with enlarged flower buds not protruded, inflorescence with corolla lobes not displayed, and inflorescence with flowers fully opened and emitting strong fragrance. Using the Illumina RNA-Seq technique, 319,425,972 clean reads were obtained and were assembled into 104,691 final unigenes (average length of 853 bp), 41.75% of which were annotated in the NCBI non-redundant protein database. Among the annotated unigenes, 36,967 were assigned to gene ontology categories and 19,956 were assigned to eukaryoticorthologous groups. Using the Kyoto Encyclopedia of Genes and Genomes pathway database, 12,388 unigenes were sorted into 286 pathways. Based on these transcriptomic data, we obtained a large number of candidate genes that were differentially expressed at different flower stages and that were related to floral pigment biosynthesis and fragrance metabolism. This comprehensive transcriptomic analysis provides fundamental information on the genes and pathways involved in flower secondary metabolism and development in S. oblata, providing a useful database for further research on S. oblata and other plants of genus Syringa.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0142542PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4654506PMC
June 2016

Ca²⁺ signal contributing to the synthesis and emission of monoterpenes regulated by light intensity in Lilium 'siberia'.

Plant Physiol Biochem 2015 Jun 21;91:1-9. Epub 2015 Mar 21.

College of Landscape Architecture, Beijing University of Agriculture, Beijing 102206, China. Electronic address:

The floral scent is an important part of plant volatile compounds, and is influenced by environmental factors. The emission of monoterpenes of Lilium 'siberia' is regulated by light intensity, but the mechanism is large unknown. In this study, the expression of Li-mTPS, a monoterpene synthase gene in the tepals of Lilium 'siberia', and net Ca(2+) flux were investigated after exposure to different levels of light intensity (0, 100, 300, 600, 1000, and 1500 μmol m(-2) s(-1)). Moreover the effect of LaCl3 and ethylene glycol-bis-(2-aminoethylether)-N,N,N',N'-tetraacetic acid (EGTA) on the Li-mTPS expression, monoterpene emission, and net Ca(2+) flux were examined at 600 μmol m(-2) s(-1). The results showed that along with the enhancement of light intensity, the expression level of Li-mTPS increased gradually, and the net Ca(2+) influx was also enhanced showing a similar pattern. It was found that LaCl3 and EGTA effectively inhibited the increase in expression of Li-mTPS and the net Ca(2+) influx induced by light treatment. Moreover, the release amounts of monoterpenes decreased significantly after treatment with LaCl3 and EGTA. So it can be concluded that Ca(2+) signal contributed to the biosynthesis and emission of monoterpenes regulated by light intensity in Lilium 'siberia' tepals. The increased light intensity firstly triggered the Ca(2+) influx to cytoplasm, and then the gene expression of monoterpene synthases downstream was activated to regulate the biosynthesis and emission of monoterpenes. But in the signaling pathway other mechanisms were thought to be involved in the emission of monoterpenes regulated by light intensity, which need to be investigated in future research.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.plaphy.2015.03.005DOI Listing
June 2015