Publications by authors named "Zahra Zareshahrabadi"

14 Publications

  • Page 1 of 1

Evaluation of Antimicrobial and Antibiofilm Activities of Copper Oxide Nanoparticles within Soft Denture Liners against Oral Pathogens.

Bioinorg Chem Appl 2021 4;2021:9939275. Epub 2021 Jun 4.

Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.

Objectives: Soft denture liners provide a favorable environment for adhesion and colonization of microorganisms. This in vitro study aimed to examine the efficacy of different concentrations of copper oxide nanoparticles (CuO NPs) incorporation into soft denture liner on the biofilm formation of the microbial species.

Methods: Field Emission Scanning Electron Microscopy (FESEM) images from NPs were recorded. Antifungal susceptibility testing of CuO NPs against five standard strains of (CBS 10261, 1905, 1912, 1949, 2730), (ATCC35668), (ATCC27607), and ATCC9222) was performed by the broth microdilution method with the Clinical and Laboratory Standards Institute reference method. The biofilm inhibition percentages of CuO NPs on the soft denture liners were determined by XTT assay.

Results: The characterization of CuO NPs by scanning electron microscope (SEM) analyses confirmed the synthesis of NPs with appropriate structure and size with a mean diameter of 18.3 ± 9.1 nm. The CuO NPs successfully inhibited the growth of the tested standard strains of nd spp. at concentrations ranging from 64 to 128 g mL. Indeed, incorporation of CuO NPs at a concentration of 500 g mL into the soft denture liners exhibited a significant activity (75%) in inhibition of . biofilm formation in a dose-dependent manner. The biofilm formation of in the presence of CuO NPs was lower than spp. in comparison with the control group ( < 0.05).

Conclusion: Incorporation of CuO NPs significantly decreased the colonization and plaque formation of the oral pathogens, especially accumulation. These NPs may be useful as a promising agent for the antimicrobial management of soft denture liner materials.
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http://dx.doi.org/10.1155/2021/9939275DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8195668PMC
June 2021

Protective Effects of the Probiotic Bacterium and a Murine Model of Oral Candidiasis.

Iran J Med Sci 2021 May;46(3):207-217

Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.

Background: Oral candidiasis is a frequent form of candidiasis, caused by species, in particular, (). The transition of from yeast to hyphae allows its attachment to epithelial cells, followed by biofilm formation, invasion, and tissue damage. Hence, we investigated the effect of subspecies thermophilus () on the growth as well as biofilm and germ-tube formation of both and in vivo in a murine model.

Methods: This experimental study was performed in the Department of Medical Mycology and Parasitology, School of Medicine, in collaboration with the Central Research Laboratory and the Comparative Biomedical Center, Shiraz University of Medical Sciences, Shiraz, Iran (2017 to 2018). The inhibitory activity of against species growth was evaluated using the broth microdilution method, and the inhibition of biofilm formation was measured using the XTT assay. The inhibition of germ-tube formation by was evaluated using the plate assay and fluorescence microscopy. The experimental activity of the probiotic bacterium was assessed by culture and histopathological methods in six groups of five mice, comprising those treated with four concentrations of probiotics, fluconazole, and distilled water. The one-way analysis of variance, followed by a hoc test, was used and a P value of less than 0.05 was considered significant.

Results: inhibited species growth at concentrations of 16 to 512 µg/mL. This probiotic inhibited the formation of biofilms and germ tubes in a dose-dependent manner. significantly reduced the colony-forming units in the mice receiving 30 mg/mL of this probiotic treatment compared with the control group (P=0.024). The histopathological analysis showed that colonization was diminished in the mice following the administration of the probiotic.

Conclusion: Given the inhibitory activity of against the growth, transition, and biofilm formation of , it could be used in the management of oral candidiasis.
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http://dx.doi.org/10.30476/ijms.2020.82080.0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8163705PMC
May 2021

Prevalence of superficial-cutaneous fungal infections in Shiraz, Iran: A five-year retrospective study (2015-2019).

J Clin Lab Anal 2021 Jul 24;35(7):e23850. Epub 2021 May 24.

Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.

Background: Superficial and cutaneous fungal infections are common in tropical areas. The aim of this study was to provide a basic database of superficial and cutaneous mycoses and the most common etiological agents among patients.

Methods: Between 2015 and 2019, a total of 1807 patients suspected of superficial and cutaneous mycosis referring to the mycology laboratory of Shiraz medical school, Fars, Iran were evaluated. Specimens were taken from the patients' affected area, and clinical samples were examined by direct microscopy and culture. The epidemiological profile of the patients was collected.

Results: A total of 750 patients were confirmed with mycoses. Positive samples totaled 750 cases consisting of the nail (373/49.7%), skin (323/43%), head (47/6.26%), and mucosal membrane (4/0.5%). The yeasts group included 304 Candida spp. (70.3%), 123 Malassezia spp. (28.47%), and 5 Rhodotorula spp. (1.1%). The filamentous fungi were distributed as 34.8% dermatophytes and 7.5% non-dermatophyte. The clinical types of dermatophytosis were tinea unguium (110/261), tinea capitis (50/261), tinea pedis (48/261), tinea corporis (37/261), and tinea cruris (16/261). Non-dermatophyte molds included A. flavus 17, A. niger 4, Aspergillus spp. 15, Penicillium. 10, Fusarium 6, Mucor 2, Stemphylium 1, and Alternaria 1.

Conclusion: This study provides useful data for the study trends of superficial and cutaneous fungal infections in a specific area. The mycological data confirmed higher incidence of candidiasis (mainly onychomycosis) and dermatophytosis in patients affected by fungal pathogens, which helped to better understand the epidemiological aspects of these mycoses.
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http://dx.doi.org/10.1002/jcla.23850DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8274978PMC
July 2021

Antimicrobial core-shell electrospun nanofibers containing Ajwain essential oil for accelerating infected wound healing.

Int J Pharm 2021 Jun 12;603:120698. Epub 2021 May 12.

Department of Medical Mycology and Parasitology, School of Medicine, Shiraz University of Medical Sciences, Shiraz 71348-45794, Iran; Basic Sciences in Infectious Diseases Research Center, Shiraz University of Medical Sciences, Shiraz 71348-45794, Iran. Electronic address:

Treatment of skin injuries is still facing major challenges, such as chronicity and infections, particularly those caused by multi-drug resistance pathogens. An effective treatment of such wounds should accelerate the wound healing process while preventing bacterial contamination. Here, a novel core-shell nanofiber mat was fabricated comprising gelatin/polyvinyl alcohol (as a core) and aloe vera/arabinose/polyvinylpyrrolidone (as a shell) for accelerating the healing process of bacteria-infected wounds. Trachyspermum Ammi (Ajwain) essential oil (EO), as a potent and natural antimicrobial agent against microorganisms, was incorporated into the core of nanofiber mats using coaxial electrospinning. The microscopy images demonstrated the successful fabrication of the core-shell structure with a uniform fiber size of 564 ± 106.35 nm. Moreover, Ajwain EO-loaded nanofiber mat (core-shell/EO) provided excellent antimicrobial activity and antioxidant ability. The in vitro and ex vivo release of Ajwain EO from the fabricated nanofiber mat corroborated a prolonged release profile. Furthermore, in vivo antibacterial activity, wound closure, and histomorphological examinations showed the high efficacy of the core-shell/EO mat in the treatment of Staphylococcus aureus-infected full-thickness rat wounds compared to standard control treatment with a gauze. Overall, these results represent the core-shell/EO mat's potential as a newly developed wound dressing for bacteria-infected full-thickness skin injuries.
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http://dx.doi.org/10.1016/j.ijpharm.2021.120698DOI Listing
June 2021

Survey of aflatoxins and ochratoxin A contamination in spices by HPLC-based method in Shiraz, Southern of Iran.

Environ Sci Pollut Res Int 2021 Mar 27. Epub 2021 Mar 27.

Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.

Among food and agricultural products, spices play important roles in the diets of millions of people worldwide. These products may be colonized by fungi genus and subsequently mycotoxin production. Due to the large demand and supply of spice for cooking, preservative effects, or medicine purpose, it is essential that further investigation is designed to examine mycotoxins in spice. In the present study, the possible contamination of spices by aflatoxins (AFTs) and ochratoxin A (OTA) were analyzed. A total of 80 spice samples (curry, sumac, ginger, and saffron) were purchased and cultured on appropriate medium. Simultaneously mycotoxins from spices were extracted with immunoaffinity columns (IAC), and the occurrence of AFTs (B1 + B2 + G1 + G2) and OTA was then determined using high-performance liquid chromatography (HPLC) with a fluorescence detector (FD). The results depicted that 62 (77.5%) and 58 (72.5%) spice samples were contaminated with AFTs and OTA, respectively. Out of the 80 analyzed spices samples, the mean concentration of AFTs and OTA was higher in the curry samples. Among spices that contaminated with mycotoxins, 5 (6.25%) and 2 (10%) of the samples were above the acceptable limit of AFTs (≥ 10 μg/kg) and OTA (≥ 15 μg/kg), respectively. Aspergillus species were the predominant species isolated, followed by Penicillium, and finally Mucor species.Among the examined samples, only few curry samples were contaminated with mycotoxins above acceptable limit. Despite this low level of contamination, this spice is used daily in the cuisine of this region of the world, and consequently, even the small amount of these heat stable toxins for a long time may cause many adverse effects. Hence, it is recommended to monitor the toxicogenous fungi contamination and level of mycotoxins in the spices.
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http://dx.doi.org/10.1007/s11356-021-13616-zDOI Listing
March 2021

Antimicrobial Activity of Thermocycled Polymethyl Methacrylate Resin Reinforced with Titanium Dioxide and Copper Oxide Nanoparticles.

Int J Dent 2021 30;2021:6690806. Epub 2021 Jan 30.

Student Research Committee, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.

Aims: This study aimed to evaluate the effect of 2.5% and 7.5% copper oxide (CuO) and titanium dioxide (TiO) nanoparticles on the antimicrobial activity of thermocycled polymethyl methacrylate (PMMA) denture base material against standard strains of yeast and bacteria species. . In this in vitro study, 150 disk-shaped (10 × 2 mm) specimens of heat-cured PMMA were prepared and divided into five groups ( = 30) to be reinforced with 2.5% CuO, 7.5% CuO, 2.5% TiO, or 7.5% TiO nanoparticles and a control group (without nanoparticle). The specimens were thermocycled, and their antimicrobial activity was assessed against standard strains of yeast including and and oral bacteria species including , , and . Data were analyzed with ANOVA and Tukey's post hoc tests ( = 0.05).

Results: Both concentrations of CuO and TiO nanoparticles had significant antimicrobial activity against , and compared with the control group ( < 0.05). Significant differences existed between both 2.5% ( = 0.006) and 7.5% CuO ( = 0.005) and the control group against . However, TiO groups were not significantly different from the control group against Concerning , 7.5% TiO was the only nanoparticle with significantly higher antimicrobial activity compared with the control group ( = 0.043).

Conclusions: Both concentrations of CuO and TiO were effective antimicrobial agents against , and , and the concentration of CuO was effective against . Yet, TiO was not much effective. Regarding , only 7.5% TiO showed efficient antimicrobial activity.
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http://dx.doi.org/10.1155/2021/6690806DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7868146PMC
January 2021

Core-shell chitosan/PVA-based nanofibrous scaffolds loaded with Satureja mutica or Oliveria decumbens essential oils as enhanced antimicrobial wound dressing.

Int J Pharm 2021 Mar 26;597:120288. Epub 2021 Jan 26.

Department of Chemical Engineering, School of Chemical and Petroleum Engineering, Shiraz University, Shiraz, Iran. Electronic address:

Wounds are prone to bacterial infections, which cause a delayed healing process. Regarding the emergence of bacterial resistance to common antibiotics, using natural antimicrobial agents can be beneficial. Chitosan is a biological polymer, which has shown partial antioxidant and antimicrobial activities. In this study, core-shell nanofibrous scaffolds composed of chitosan (CS)/polyvinyl alcohol (PVA) as the core and polyvinylpyrrolidone (PVP)/ maltodextrin (MD) as the shell were developed. Satureja mutica (S. mutica) or Oliveria decumbens (O. decumbens) essential oil (EO) was encapsulated into the core of the produced scaffolds. The broth microdilution analysis showed significant antimicrobial activity of the EOs. The SEM analysis indicated that the unloaded and loaded core-shell scaffolds with S. mutica or O. decumbens EO had a uniform, beadless structure with fiber mean diameters of 210 ± 50, 250 ± 45, and 225 ± 46 nm, respectively. The CS/PVA-PVP/MD and CS/PVA/EO-PVP/MD scaffolds indicated suitable mechanical properties. The addition of the studied EOs enhanced the antioxidant activity of the scaffolds. The antimicrobial test of produced scaffolds showed that loading of 10% S. mutica or O. decumbens EO could broaden the microbicidal activity of the CS/PVA-PVP/MD scaffolds. These results revealed that the CS/PVA/EO-PVP/MD nanofibrous scaffolds are promising candidates for wound dressing.
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http://dx.doi.org/10.1016/j.ijpharm.2021.120288DOI Listing
March 2021

Concomitant of Pulmonary Hydatid Cyst and Aspergilloma: A Rare Coinfection.

Case Rep Infect Dis 2020 12;2020:6650478. Epub 2020 Dec 12.

Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.

The coexistence of cystic echinococcosis (CE) and aspergilloma is rather uncommon. species, saprophytic fungi, can colonize pulmonary cavities that are caused by tuberculosis, sarcoidosis, and CE. Infection by is often occurring in immunosuppressed patients. However, coinfection of aspergilloma with pulmonary hydatid cyst is very unusual, especially in an immunocompetent patient with unruptured cyst. Herein, we report a case of lung hydatid cyst coinfected with in a 42-year-old Iranian man from Southern Iran. Chest X-ray and computed tomography (CT) scan showed a circumscribed cystic lesion in the superior and inferior segment of the lower lobes of right and left lungs that suggests hydatid cyst. Radical surgery (lobectomy) was performed for the patient. Histopathological evaluation reconfirmed the classical laminated layer of hydatid cyst. Moreover, the ectocyst layer of the right lung showed the presence of numerous cluster septate hyphae with acute-angled branching, as seen in the morphology of species. DNA was extracted from the cyst, and the ITS1-5.8s-ITS2 region of the fungal agent was amplified. Sequencing and analysis of seminested PCR product revealed that the isolate has the most similarity with . Further attention is recommended to control fungal pathogens during pulmonary hydatidosis. The coexistence of aspergilloma should always be kept in mind for the better management of CE.
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http://dx.doi.org/10.1155/2020/6650478DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7787855PMC
December 2020

Clinical and microbiological features of candiduria in critically ill adult patients in Shiraz, Iran (2016-2018): deviations from international guidelines and fluconazole therapeutic failure.

Med Mycol 2020 Dec 10. Epub 2020 Dec 10.

Basic Sciences in Infectious Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.

Candiduria is common among patients admitted to intensive care units (ICUs); however, clinical and microbiological data are limited, which accounts for non-compliance with international guidelines, including over treatment of asymptomatic candiduria that promotes antifungal resistance. This prospective study included adult patients admitted to ICUs of five referral hospitals in Shiraz, Iran, during 2016-2018. Species were identified by MALDI-TOF MS, and antifungal susceptibility was assessed according to CLSI M27-A3/S4. Among 2086 patients, 162 and 293 developed candiduria and bacteriuria, respectively. In total, 174 yeast isolates were collected; 88.5% were Candida albicans (91/174; 52.2%), C. glabrata (38/174; 21.8%), and C. tropicalis (25/174; 14.3%). Antifungal resistance was rare; only two isolates (one C. tropicalis and one C. krusei) were fluconazole resistant. Symptomatic candiduria was noted in 31.4% of patients (51/162); only 37% (19/51) of them were treated and 36.82% (7/19) showed fluconazole therapeutic failure. Two symptomatic patients developed candidemia shortly after candiduria. Among asymptomatic patients, 31.5% (35/111) were overtreated with fluconazole. The mortality rate was 25.3% (41/162); it did not differ between symptomatic and asymptomatic patients. Our results indicate that deviation from standard-of-care treatment for candiduria is a matter of concern given the high rate of fluconazole therapeutic failure among patients with symptomatic candiduria.

Lay Summary: Candiduria is an underestimated clinical presentation among critically ill patients and detailed data are scarce in this regard. Given the high rate of fluconazole therapeutic failure and development of candidemia in some cases, the mistreatment of candiduria should not be overlooked by clinicians.
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http://dx.doi.org/10.1093/mmy/myaa092DOI Listing
December 2020

Molecular identification and antifungal susceptibility among clinical isolates of dermatophytes in Shiraz, Iran (2017-2019).

Mycoses 2021 Apr 20;64(4):385-393. Epub 2020 Dec 20.

Department of Medical Mycology and Parasitology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.

Dermatophytosis is a common superficial mycotic infection affecting individual's quality of life worldwide. The present study aimed to perform species-level identification and evaluate the antifungal susceptibility patterns of dermatophytes isolated in Shiraz, Iran. This cross-sectional study was conducted on clinical samples collected during 2017-2019 from 307 patients suspected of having dermatophytosis. The isolates were identified by direct microscopy, culture and internal transcribed spacer ribosomal DNA sequencing, and their antifungal susceptibility patterns were determined by the microdilution method. Among 307 patients, dermatophytosis was diagnosed by microscopy in 190 (61.8%) subjects and confirmed in 130 (42.3%) cases by both microscopy and culture. It was found out tinea pedis was the most common clinical manifestation, and Trichophyton mentagrophytes was the most prevalent species (28.4%), followed by T tonsurans (23.8%), Microsporum canis (11.5%), T interdigitale (10%), T verrucosum (6.9%), T rubrum (6.9%), T benhamiae (4.6%), T violaceum (3%), T simii (3%), Epidermophyton floccosum (0.7%) and M ferrugineum (0.7%). Moreover, it was revealed that luliconazole with a geometric mean (GM) minimum inhibitory concentration (MIC) of 0.03 μg ml was the most effective agent against all tested isolates. Regardless of species, 30% of isolates responded to high MICs of griseofulvin (MIC  > 2 μg ml ). The increasing prevalence of nonindigenous species of T simii, T benhamiae and M ferrugineum in Shiraz, Iran, was a notable finding. In addition, infections due to zoophilic species showed an increasing trend. These epidemiological data, along with antifungal susceptibility patterns, may have implications for clinical decision-making and successful treatment.
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http://dx.doi.org/10.1111/myc.13226DOI Listing
April 2021

High detection of virulence factors by Candida species isolated from bloodstream of patients with candidemia.

Microb Pathog 2020 Dec 17;149:104574. Epub 2020 Oct 17.

Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran; Basic Sciences in Infectious Diseases Research Center, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.

Objective: Candida species are the normal inhabitants of the skin and mucosa that cause a wide range of debilitating diseases in immunocompromised patients and other susceptible individuals. The present study aimed to evaluate the production of exoenzymes and the biofilm formation capacity of Candida species isolated from candidemia.

Materials And Methods: In this study, a total of 100 stock Candida species isolates consist of 50 Candida albicans and 50 non-Candida albicans Candida species (24 C. glabrata, 15 C. parapsilosis, 5 C. dubliniensis, 3 C. tropicalis, 2 C. krusei and 1 C. fabianii) which previously were recovered from patients with candidemia were used. The enzymatic activity tests for hemolysin, proteinase, and phospholipase were performed by using blood Sabouraud dextrose agar, bovine serum albumin medium and egg yolk agar, respectively. Biofilm formation was determined by microplate assay method.

Result: All of the Candida albicans species could produce hemolysin. The predominant enzyme activity of species included strong and very strong levels of phospholipase, proteinase and hemolysin activity were belonged to Candida albicans isolates. There were statistically significant differences in hemolysin (P < 0.001), proteinase (P = 0.003) and phospholipase (P < 0.001) activity between two groups of albicans and non-albicans species. The biofilm formation was seen in 30 (60%) of C. albicans and 49 (98%) of non-C. albicans species. There was significant statistical differences between the two groups of isolates in biofilm formation (P < 0.001).

Conclusion: It is clear that Candida species have ability to produce several enzymes as virulence factors to contribute its pathogenicity. There were significant differences in virulence factors between the two C. albicans and non- C. albicans group. The ability for biofilm formation and producing exo-enzyme were an important virulence factors in Candida species isolates. This differences found in this report might have role in severity of disease caused by different species.
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http://dx.doi.org/10.1016/j.micpath.2020.104574DOI Listing
December 2020

Comparative Analysis of Virulence Factors of Homozygous and Heterozygous Strains of Vaginal Isolates.

Int J Microbiol 2020 27;2020:8889224. Epub 2020 Jun 27.

Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, 7134845794, Shiraz, Iran.

Although the epidemiology of pathogenic species is changing due to invasive diseases, has become the common cause of human infections worldwide. is a diploid yeast with a mostly clonal mode of reproduction and without known complete sexual cycle. This species has two heterozygous and homozygous strains at hyphal wall protein 1 gene locus (). Little is known about virulence factors of these strains. The aim of this study was to evaluate the exoenzyme activity of heterozygous and homozygous strains. A total of 60 stock species isolates, which consisted of 30 homozygous and 30 heterozygous strains, were used for exoenzyme activities. We used egg yolk agar, Sabouraud blood agar, and bovine serum albumin agar for evaluation of phospholipase, hemolysin, and proteinase activity, respectively. Homozygous strains of had more phospholipase and proteinase activity than heterozygous strains. However, there were no significant statistical differences between the two strains in the severity of exoenzymes production. Beta hemolysin activity was seen in 100% and 96.7% of the homozygous and heterozygous strains, respectively. The results of this study indicated that both of the strains exhibited exoenzyme activities in different ranges. There were no significant statistical differences in virulence factors between the homozygous and heterozygous strains.
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http://dx.doi.org/10.1155/2020/8889224DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7336225PMC
June 2020

Evaluation of Molecular Epidemiology, Clinical Characteristics, Antifungal Susceptibility Profiles, and Molecular Mechanisms of Antifungal Resistance of Iranian Species Complex Blood Isolates.

Front Cell Infect Microbiol 2020 21;10:206. Epub 2020 May 21.

Division of Hygiene and Medical Microbiology, Medical University of Innsbruck, Innsbruck, Austria.

Clonal expansion of fluconazole resistant (FLZ-R) isolates is increasingly being identified in many countries, while there is no study exploring the antifungal susceptibility pattern, genetic diversity, and clinical information for Iranian blood isolates. species complex blood isolates ( = 98) were recovered from nine hospitals located in three major cities, identified by MALDI-TOF MS, and their genetic relatedness was examined by AFLP fingerprinting. Antifungal susceptibility testing followed CLSI-M27-A3 and and hotspots 1/2 (HS1/2) of were sequenced to assess the azole and echinocandin resistance mechanisms, respectively. Ninety-four and four isolates were identified from 90 patients. Only 43 patients received systemic antifungal drugs with fluconazole as the main antifungal used. The overall mortality rate was 46.6% (42/90) and death mostly occurred for those receiving systemic antifungals (25/43) relative to those not treated (17/47). Although, antifungal-resistance was rare, one isolate was multidrug-resistant (FLZ = 16 μg/ml and micafungin = 8 μg/ml) and the infected patient showed therapeutic failure to FLZ prophylaxis. Mutations causing azole and echinocandin resistance were not found in the genes studied. AFLP revealed five genotypes (G) and G1 was the main one (59/94; 62.7%). Clinical outcome was significantly associated with city ( = 0.02, α <0.05) and Mashhad was significantly associated with mortality ( = 0.03, α <0.05). Overall, we found a low level of antifungal resistance for Iranian blood isolates, but the noted MDR strain can potentially become the source of future infections and challenge the antifungal therapy in antifungal-naïve patients. AFLP typing results warrants confirmation using other resolutive typing methods.
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http://dx.doi.org/10.3389/fcimb.2020.00206DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7253641PMC
June 2021

Chemical compositions and antifungal activities of against and species.

Curr Med Mycol 2019 ;5(4):20-25

Department of Medical Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.

Background And Purpose: Despite the various applications of species, there are limited data in this domain. Regarding this, the present study was conducted to investigate the essential oil (EO) biological activity of species in Iran.

Materials And Methods: The EO of flowers was obtained by hydrodistillation. Chemical compositions of the EO were analyzed using gas chromatography-mass spectrometry. In addition, minimum inhibitory concentrations (MIC) were measured by means of the broth microdilution method. The estimation of antibiofilm and cytotoxic activities was also accomplished using the tetrazolium salt and MTT assays, respectively.

Results: A total of 26 components were identified in the EO with linalool as the main constituent (28.46%). A MIC range value of 0.25-8 μL/mL was obtained against all of the tested fungi. The EO inhibited the biofilm development of the tested strains at a concentration of 4-8 μL/mL. Cytotoxicity (IC) of EO against the HeLa cell was greater than the MIC concentration (6.49 μL/mL).

Conclusion: Based on the findings, it was concluded that the EO of has the potential for further use as an antifungal agent.
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http://dx.doi.org/10.18502/cmm.5.4.2162DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7034783PMC
January 2019
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