Publications by authors named "Z X You"

1,542 Publications

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Observation of synergistic antibacterial properties of prodigiosin from jx-1 with metal ions in clinical isolates of .

Prep Biochem Biotechnol 2021 Jul 21:1-7. Epub 2021 Jul 21.

College of Biological, Chemical Sciences and Engineering, Jiaxing University, Jiaxing, China.

Methicillin-resistant (MRSA) infections are a major global health problem, and novel and effective antimicrobial drugs are urgently required to combat this life-threatening pathogen. Prodigiosin (PG) is a bacterial secondary metabolite with excellent anticancer and antibacterial properties. However, little is known about the antibacterial function of PG against MRSA. Therefore, the antibacterial efficacy of PG alone and PG in combination with different metal ions against clinic isolates of MRSA and methicillin-sensitive (MSSA) strain was evaluated in the present study. The minimum inhibitory concentration of PG against both MRSA and MSSA was 0.25 μg/mL. However, 0.1 μg/mL PG showed a stronger inhibitory effect on MSSA cell growth (47.12%) than on MRSA cell growth (35.87%). Surprisingly, we observed a significant difference ( < 0.01) in membrane integrity between PG-treated MRSA and MSSA using the propidium iodide staining assay. Further, we found that in combination with PG, Zn, Al, and Cu showed synergistic antibacterial effects against MRSA and MSSA. Our results could increase the current knowledge regarding the efficacy of PG in inhibiting the growth of different types of clinical isolates and also offer a novel strategy for developing efficient antibacterial agents.
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http://dx.doi.org/10.1080/10826068.2021.1944201DOI Listing
July 2021

Self-healing polyurethane-elastomer with mechanical tunability for multiple biomedical applications in vivo.

Nat Commun 2021 07 20;12(1):4395. Epub 2021 Jul 20.

Department of Cardiovascular Surgery, Ruijin Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai, China.

The unique properties of self-healing materials hold great potential in the field of biomedical engineering. Although previous studies have focused on the design and synthesis of self-healing materials, their application in in vivo settings remains limited. Here, we design a series of biodegradable and biocompatible self-healing elastomers (SHEs) with tunable mechanical properties, and apply them to various disease models in vivo, in order to test their reparative potential in multiple tissues and at physiological conditions. We validate the effectiveness of SHEs as promising therapies for aortic aneurysm, nerve coaptation and bone immobilization in three animal models. The data presented here support the translation potential of SHEs in diverse settings, and pave the way for the development of self-healing materials in clinical contexts.
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http://dx.doi.org/10.1038/s41467-021-24680-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8292539PMC
July 2021

A long-lasting negative effect of COVID-19 pandemic on public sentiment: a full-year tracking of online public sentiment in mainland China.

J Med Internet Res 2021 Jul 12. Epub 2021 Jul 12.

Shanghai Key Laboratory of Mental Health and Psychological Crisis Intervention, Key Laboratory of Brain Functional Genomics (Ministry of Education), the Institute of Brain and Education Innovation, School of Psychology and Cognitive Science, East China Normal University, Room 309, Old Library Building, No. 3663, North Zhongshan Road, Shanghai, Shanghai, CN.

Background: The outbreak of COVID-19 induces negative emotions among people. These emotions are expressed by humans on social media and rapidly spread through the Internet. The spread could cause a wide range of panic in the public. Understanding the change of online public sentiments during the pandemic provides valuable information for developing appropriate policies to reduce the negative impact of the pandemic on the public.

Objective: Previous studies consistently showed a devastating negative impact of COVID-19 on public sentiments after the outbreak. However, it remains unclear the variation of public sentiment during the recovery phase in the pandemic.

Methods: In the current study, we continuously tracked online public sentiment through the year 2020 and collected 64,723,242 posts across China's largest social media platform Sina Weibo. We conducted sentiment analysis based on natural language processing to analyze the emotions reflected on posts.

Results: We found that the pandemic not only affected online public sentiment in the initial outbreak but also induced long-term negative effects even in the recovery period. The long-term negative effect was no longer correlated with the number of new confirmed cases both locally and nationwide during the recovery period and was not caused by the post-pandemic economic recession.

Conclusions: COVID-19 induces long-term negative effects on public sentiments even the country recovers from the pandemic. It reminds public health and government administrators of the need to pay attention to public mental health even once the pandemic has concluded.

Clinicaltrial:
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http://dx.doi.org/10.2196/29150DOI Listing
July 2021

Feasibility of singlicate-based analysis in bridging ADA assay on Meso-Scale Discovery platform: comparison with duplicate analysis.

Bioanalysis 2021 Jul 19;13(14):1123-1134. Epub 2021 Jul 19.

BioMedicine Design, Pfizer Inc., Andover, MA 01810, USA.

To investigate the feasibility of singlicate analysis in anti-drug antibody (ADA) assay by comparing performance characteristics for assays qualified in duplicate and singlicate formats. We employed modeling to assess and quantify the impact of singlicate to cut point factor (CPF) in scenarios with the duplicate precision from 1-20% and the proportion of well-to-well variance to overall assay variance from 0.01-0.90. The impact to CPF by singlicate is marginal if the well-to-well coefficient of variation is <10% and represents <25% of the overall variability. The assay parameters including sensitivity, precision, selectivity, drug and target tolerance were comparable between singlicate and duplicate based assays. Our results suggested the minimal impact of singlicate analysis on ADA assay with good duplicate precision. The study provided additional supportive evidence that the singlicate-based analysis is feasible in ADA ligand binding assays.
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http://dx.doi.org/10.4155/bio-2021-0095DOI Listing
July 2021

Effects of sodium humate and glutamine combined supplementation on growth performance, diarrhea incidence, blood parameters, and intestinal microflora of weaned calves.

Anim Sci J 2021 Dec;92(1):e13584

Heilongjiang Key Laboratory of Experimental Animals and Comparative Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.

This experiment was conducted to investigate the effects of sodium humate (HNa) and glutamine (Gln) alone or combined supplementation on growth performance, diarrhea incidence, blood parameters, and intestinal microflora of weaned Holstein calves. In a 14-day experiment, 40 calves at 51 ± 3 days of age were randomly allocated to four treatment groups: (1) NC (basal diet), (2) NC + 5% HNa, (3) NC + 1% Gln, and (4) NC + 5% HNa + 1% Gln. Calves combined supplementation with HNa and Gln had a higher (P < .05) ADG, serum concentration of glucose (GLU), IgA, and IgG but lower fecal scores, diarrhea incidence, serum concentration of TNF-α, and IL-10 compared with NC group (P < .05). Compared with NC group, HNa + Gln group showed higher (P < .05) serum GSH and T-AOC activities but lower (P < .05) concentration of MDA and D-lac. Furthermore, the abundances of Prevotella ruminicola, Bifidobacterium, and Lactobacillus in rectal digesta were increased (P < .05), but the Escherichia coli was significantly decreased. In conclusion, combined supplementation with HNa and Gln can effectively improve the immune status, antioxidant capacity, and intestinal microflora of the weaned calves while reducing diarrhea incidence.
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http://dx.doi.org/10.1111/asj.13584DOI Listing
December 2021