Publications by authors named "Yvonne Stedman"

4 Publications

  • Page 1 of 1

Combined Effect of Temperature and Relative Humidity on the Survival of Isolates on Stainless Steel Coupons.

Int J Environ Res Public Health 2022 01 14;19(2). Epub 2022 Jan 14.

College of Health & Life Sciences, Aston University, Birmingham B4 7ET, UK.

The survival on stainless steel of ten isolates from food factory, clinical and veterinary sources was investigated. Stainless steel coupons inoculated with were dried and stored at a range of temperatures and relative humidity (RH) levels representing factory conditions. Viability was determined from 1 to 22 days. Survival curves obtained for most isolates and storage conditions displayed exponential inactivation described by a log-linear model. Survival was affected by environmental temperatures and RH with decimal reduction times (DRTs) ranging from <1 day to 18 days. At 25 °C/15% RH, all isolates survived at levels of 10 to 10 cfu for >22 days. Furthermore, temperatures and RH independently influenced survival on stainless steel; increasing temperatures between 10 °C and 37 °C and increasing RH levels from 30-70% both decreased the DRT values. Survival curves displaying a shoulder followed by exponential death were obtained for three isolates at 10 °C/70% RH. Inactivation kinetics for these were described by modified Weibull models, suggesting that cumulative injury occurs before cellular inactivation. This study highlights the need to control temperature and RH to limit microbial persistence in the food manufacturing environment, particularly during the factory shut-down period for cleaning when higher temperature/humidity levels could be introduced.
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http://dx.doi.org/10.3390/ijerph19020909DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8776188PMC
January 2022

Pet Food Factory Isolates of Serotypes Do Not Demonstrate Enhanced Biofilm Formation Compared to Serotype-Matched Clinical and Veterinary Isolates.

Biomed Res Int 2019 29;2019:8569459. Epub 2019 Jan 29.

School of Life & Health Sciences, Aston University, Birmingham, B4 7ET, UK.

Environmentally persistent in the pet food factory environment has been described, with biofilm formation suggested as a candidate mechanism contributing to their persistence. In this study the ability of a panel of isolates from factory, clinical, and veterinary sources was investigated for their ability to form biofilms at 24 and 48 hours. The effect of nutrient availability and incubation time on biofilm formation was investigated using full strength and diluted 1/20 TSB media at 37°C, 25°C, 15°C, and 10°C. Results highlighted that all the isolates were able to form biofilms in both nutrient conditions and this was highly correlated with temperature. At 25°C, biofilm formation was enhanced in diluted 1/20 TSB and increased incubation time (48h) (p= <0.001). However, this was not observed at 10°C, 15°C, or 37°C. None of the factory isolates demonstrated enhanced biofilm formation in comparison to serotype-matched isolates from veterinary and clinical sources. Senftenberg 775W was the strongest biofilm former at 15°C, 25°C, and 37°C in all the conditions tested (p=<0.05). Biofilm formation is an important mechanism of environmental persistence in the food manufacturing environment; however, there is no evidence of an enhanced biofilm-producing phenotype in factory persistent strains.
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http://dx.doi.org/10.1155/2019/8569459DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6374821PMC
June 2019

The role of Advanced Nurse Practitioners in the availability of abortion services.

Best Pract Res Clin Obstet Gynaecol 2010 Oct 10;24(5):569-78. Epub 2010 Apr 10.

Sexual and Reproductive Health, Liverpool Primary Care (NHS) Trust, Liverpool, UK.

Despite the legalisation of abortion in many countries worldwide, access to abortion is often restricted in many ways. Lack of availability of trained and willing physicians, inadequate and poor infrastructure as well as affordability are issues that are still contributing to poor access to abortion for many women living in countries that have legalised abortion. Improving access to early abortion despite the declining number of doctors willing to provide abortions is being addressed in some countries by expanding the role of advanced nurse-midwife practitioners in this field. There is good evidence to suggest that the outcome of first-trimester abortions performed by suitably trained non-medical practitioners is comparable in terms of safety and efficacy to abortions performed by doctors. These mid-level practitioners also have a key role in providing post-abortion care and contraception to women. We need to address outdated laws and regulations as well as political challenges that restrict both the ability of advanced nurse-midwife practitioners to provide abortion care and the opportunities to train them appropriately.
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http://dx.doi.org/10.1016/j.bpobgyn.2010.02.014DOI Listing
October 2010

Pharmacists and POEC.

J Fam Plann Reprod Health Care 2003 Oct;29(4):249; author reply 249

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http://dx.doi.org/10.1783/147118903101197926DOI Listing
October 2003
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