Publications by authors named "Yuying Yang"

64 Publications

Flavonoids derived from buckwheat hull can break advanced glycation end-products and improve diabetic nephropathy.

Food Funct 2021 Jun 25. Epub 2021 Jun 25.

College of Food Science and Engineering, Jilin Agricultural University, Changchun, 130118, Jilin, China. and National Engineering Laboratory for Wheat and Corn Deep Processing, Changchun, 130118, Jilin, China.

Diabetic nephropathy (DN) is the most important complication in patients with diabetes. The accumulation of advanced glycation end-products (AGEs) is the main reason for the development of DN. In this study, we investigated the mechanism of buckwheat hull flavonoids to break AGEs in vitro by measuring fluorescence analysis, three-dimensional fluorescence, protein molecular weight, free amino groups, and the sulfhydryl group content. Proteomics analysis was used to determine the effect of total buckwheat hull flavonoids (TBHF) intervention on protein differential expression in the kidney of db/db mice. The results showed that buckwheat hull flavonoids were potent in breaking AGEs in vitro, and they protected mice kidneys by regulating the renal AGE-RAGE pathway. This study lays a strong experimental and theoretical foundation for the development of new lysing agents to break AGEs. The findings should make an important contribution to the field of flavonoids in improving the application of diabetic nephropathy in the diet.
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http://dx.doi.org/10.1039/d1fo01170gDOI Listing
June 2021

Dietary supplementary glutamine and L-carnitine enhanced the anti-cold stress of Arbor Acres broilers.

Arch Anim Breed 2021 4;64(1):231-243. Epub 2021 Jun 4.

National Experimental Teaching Demonstration Center of Animal Medicine Foundation, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, 163319, PR China.

Newborn poultry in cold regions often suffer from cold stress, causing a series of changes in their physiology and metabolism, leading to slow growth and decreased production performance. However, a single anti-stress substance cannot completely or maximally eliminate or alleviate the various effects of cold stress on animals. Therefore, the effects of the supplemented glutamine and L-carnitine on broilers under low temperature were evaluated in this study. Broilers were randomly allocated into 16 groups which were respectively fed with different levels of glutamine and L-carnitine according to the L (4 ) orthogonal experimental design for 3 weeks (the first week is the adaptive feeding period; the second and third weeks are the cold exposure period). Growth performance was recorded, and blood samples were collected during cold exposure. The results showed the supplementation had altered the plasma parameters, growth performance and cold-induced oxidative stress. The increase of corticosterone and suppression of thyroid hormone was ameliorated. Supplemented groups had lower daily feed intake and feed-to-gain ratio, higher daily weight gain and better relative weights of immune organs. Plasma glucose, total protein, blood urea nitrogen and alkaline phosphatase changed as well. Oxidative stress was mollified due to the improved activities of superoxide dismutase and glutathione peroxidase, heightened total antioxidant capacity and stable malondialdehyde. Dietary glutamine and L-carnitine improve the growth performance, nutritional status and cold stress response of broilers at low temperature, and their interaction occurred.
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http://dx.doi.org/10.5194/aab-64-231-2021DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8209504PMC
June 2021

The CdIn S /WO Nanosheet Composite Has a Significantly Enhanced Photo-electrochemical Cathodic Protection Performance and Excellent Electron Storage Capability.

Chemistry 2021 Jun 17. Epub 2021 Jun 17.

Key Laboratory of Marine Environmental Corrosion and Bio-fouling Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao, 266071, P. R. China.

Photo-electrochemical cathodic protection (CP) technology is considered to be a green metallic corrosion protection technology that uses solar energy to protect from corrosion and does not consume any anode materials. In this work, a CdIn S /WO nanocomposite photoelectrode was prepared, and its photo-electrochemical CP performance and mechanism were studied and analyzed. WO has a well band matching with CdIn S , leading to a significantly enhanced photo-electrochemical CP performance of the nanocomposite. Meanwhile, as confirmed in this work, the CdIn S /WO nanocomposite can store photoinduced electrons under light illumination through intercalation reactions and changing the valence state of tungsten. Moreover, it can discharge in the dark state to provide continuous CP for the coupled metals. This research will promote the practical application process of the photo-electrochemical CP technology.
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http://dx.doi.org/10.1002/chem.202101479DOI Listing
June 2021

Combination of UPLC-Q-TOF/MS and Network Pharmacology to Reveal the Mechanism of Qizhen Decoction in the Treatment of Colon Cancer.

ACS Omega 2021 Jun 26;6(22):14341-14360. Epub 2021 May 26.

College of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China.

Traditional Chinese medicine (TCM) has been utilized for the treatment of colon cancer. Qizhen decoction (QZD), a potential compound prescription of TCM, possesses multiple biological activities. It has been proven clinically effective in the treatment of colon cancer. However, the molecular mechanism of anticolon cancer activity is still not clear. This study aimed to identify the chemical composition of QZD. Furthermore, a collaborative analysis strategy of network pharmacology and cell biology was used to further explore the critical signaling pathway of QZD anticancer activity. First, ultraperformance liquid chromatography-quadrupole time-of-flight/mass spectrometry (UPLC-Q-TOF/MS) was performed to identify the chemical composition of QZD. Then, the chemical composition database of QZD was constructed based on a systematic literature search and review of chemical constituents. Moreover, the common and indirect targets of chemical components of QZD and colon cancer were searched by multiple databases. A protein-protein interaction (PPI) network was constructed using the String database (https://www.string-db.org/). All of the targets were analyzed by Gene Oncology (GO) bioanalysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, and the visual network topology diagram of "Prescription-TCM-Chemical composition-Direct target-Indirect target-Pathway" was constructed by Cytoscape software (v3.7.1). The top molecular pathway ranked by statistical significance was further verified by molecular biology methods. The results of UPLC-Q-TOF/MS showed that QZD had 111 kinds of chemical components, of which 103 were unique components and 8 were common components. Ten pivotal targets of QZD in the treatment of colon cancer were screened by the PPI network. Targets of QZD involve many biological processes, such as the signaling pathway, immune system, gene expression, and so on. QZD may interfere with biological pathways such as cell replication, oxygen-containing compounds, or organic matter by protein binding, regulation of signal receptors or enzyme binding, and affect cytoplasm and membrane-bound organelles. The main antitumor core pathways were the apoptosis metabolic pathway, the PI3K-Akt signal pathway, and so on. Expression of the PI3K-Akt signal pathway was significantly downregulated after the intervention of QZD, which was closely related to the inhibition of proliferation and migration of colon cancer cells by cell biology methods. The present work may facilitate a better understanding of the effective components, therapeutic targets, biological processes, and signaling pathways of QZD in the treatment of colon cancer and provide useful information about the utilization of QZD.
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http://dx.doi.org/10.1021/acsomega.1c01183DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8190929PMC
June 2021

Preparation of a novel monoclonal antibody against Avian leukosis virus subgroup J Gp85 protein and identification of its epitope.

Poult Sci 2021 Jul 12;100(7):101108. Epub 2021 Mar 12.

School of Animal Science, Yangtze University, Jingzhou 434025, China. Electronic address:

Avian leukosis virus subgroup J (ALV-J) is an avian oncogenic retrovirus that has caused huge economic losses in the poultry industry due to its great pathogenicity and transmission ability. However, the continuous emergence of new strains would bring challenges to diagnosis and control of ALV-J. .This study focuses on preparing the monoclonal antibody (MAb) against ALV-J Gp85 and identifying its epitope. The truncated ALV-J gp85 gene fragment was amplified and then cloned into expression vectors. Purified GST-Gp85 was used to immune mice and His-Gp85 was used to screen MAb. Finally, a hybridoma cell line named J16 that produced specific MAb against the ALV-J. Immunofluorescence assay showed that MAb J16 specifically recognized ALV-J rather than ALV-A or ALV-K infected DF-1 cells. To identify the epitope recognized by MAb J16, fourteen partially overlapping ALV-J Gp85 fragments were prepared and tested by Western blot. The results indicated that peptide 150-LIRPYVNQ-157 was the minimal epitope of ALV-J Gp85 recognized by MAb J16. Alignment analysis of Gp85 from different ALV subgroups showed that the epitope keep high conservation among 36 ALV-J strains, but significant different from that of ALV subgroup A, B, C, D, E and K. Overall, we prepared a MAb specific against ALV-J and identified peptide 150-LIRPYVNQ-157 as a novel specific epitope of ALV-J Gp85, which may assist in laying the foundation for specific ALV-J detection methods.
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http://dx.doi.org/10.1016/j.psj.2021.101108DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8192869PMC
July 2021

Dispose waste liquor of fresh biomass of a hyperaccumulator in phytoextraction process.

Int J Phytoremediation 2021 May 18:1-11. Epub 2021 May 18.

Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, China.

has been widely employed to remove cadmium (Cd) and zinc (Zn) from contaminated soils and harvested biomass is used to recover valuable metals. While chopping and compacting are efficient methods to rapidly reduce the volume and moisture of fresh biomass, the resulting waste liquor containing metals needs treatment. Two types of contaminated soils were cropped with and top-dressed with this liquor to study metals migration in soil profile and their uptake by plants. There were three treatments: planting and adding liquor (PL), planting without liquor (P) and adding liquor without planting (L). The results showed that Cd and Zn from liquor were mainly retained at top soil 0-10 cm under L treatment. Compared with L treatment, soil Cd and Zn under PL treatment decreased significantly in soil profile due to the extraction of . Moreover, the amount of Cd and Zn extracted by plants was greater than that applied in soils. The metal removal rate by in acid clay loam soil was higher than that in neutral sandy soil. To sum up, metal retaining in soil and uptake by can be used to treat waste liquor from its fresh biomass. Rapid and efficient treatment of harvested fresh biomass is still a challenge although phytoextraction using hyperaccumulator has been widely employed. Chopping and compacting fresh biomass are efficient methods for rapid dehydration, however, a large amount of waste liquor that contains of Cd and Zn is produced and needs treatment. In the present study, a simple and low-cost method was tested to dispose the liquor, i. e. irrigating it onto the surface of contaminated soils where grown . It was found that Cd and Zn applied in soils from liquor were mainly retained at top 0-10 cm soil depth where root system was widespread, and the amount of Cd and Zn extracted by plants was greater than that applied in soils. Therefore, it is technically feasible to dispose the waste liquor dewatering from fresh biomass of in its phytoextraction process. This study is helpful for the rapid, efficient and low-cost treatment of harvested fresh biomass in the large-scale application of phytoremediation.
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http://dx.doi.org/10.1080/15226514.2021.1917509DOI Listing
May 2021

Corrigendum to "High prevalence and persistence of carbapenem and colistin resistance in livestock farm environments in China".

J Hazard Mater 2021 Sep 11;417:125951. Epub 2021 May 11.

Beijing Advance Innovation Center for Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.

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http://dx.doi.org/10.1016/j.jhazmat.2021.125951DOI Listing
September 2021

Inhibition of Long Non-Coding RNA KCNQ1OT1 Attenuates Neuroinflammation and Neuronal Apoptosis Through Regulating NLRP3 Expression via Sponging miR-30e-3p.

J Inflamm Res 2021 5;14:1731-1742. Epub 2021 May 5.

Department of Neurology, The First Affiliated Hospital of Hebei North University, Zhangjiakou, Hebei, 075000, People's Republic of China.

Background: Neuroinflammation and neuronal apoptosis are considered as the critical factors in the pathogenesis of multiple neurological diseases. Recent studies have shown that long non-coding RNA (lncRNA) plays a crucial part in neuroinflammation and neuronal apoptosis.

Methods: The expression levels of lncRNA KCNQ1OT1, miR-30e-3p and NLRP3 in lipopolysaccharide (LPS)-induced HMC3 cells were analyzed using RT-qPCR. MTT assay, LDH release assay and ELISA were used to assess the effect of KCNQ1OT1 and miR-30e-3p on neuroinflammation and neuronal apoptosis. The targeted regulatory relationships among KCNQ1OT1, miR-30e-3p and NLRP3 were evaluated by bioinformatics analysis, dual-luciferase reporter gene assay, RT-qPCR and Western blot.

Results: In LPS-induced HMC3 cells, the expression levels of KCNQ1OT1 and NLRP3 were increased, while the expression level of miR-30e-3p was reduced. Knockdown of KCNQ1OT1 alleviated LPS-induced apoptosis and neuroinflammation of HMC3 cells, accompanied by increased cell viability, low LDH release and reduced cell apoptosis rate, and reduced levels of TNF-α, IL-1β and IL-6. Overexpression of miR-30e-3p had a similar effect. Additionally, KCNQ1OT1 could bind with miR-30e-3p and repress its expression in HMC3 cells, and KCNQ1OT1 overexpression counteracted miR-30e-3p's inhibitory effect on LPS-induced neuronal damage and inflammatory response in HMC3 cells. Furthermore, KCNQ1OT1 could positively regulate the expression of NLRP3 via repressing miR-30e-3p.

Conclusion: Inhibition of KCNQ1OT1 could reduce neuroinflammation and neuronal apoptosis induced by LPS in HMC3 cells by regulating miR-30e-3p/NLRP3 pathway, suggesting that KCNQ1OT1 and miR-30e-3p could serve as promising therapeutic targets for treating neurological diseases.
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http://dx.doi.org/10.2147/JIR.S291274DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107009PMC
May 2021

[Advances in the research of enterobacterial common antigen].

Sheng Wu Gong Cheng Xue Bao 2021 Apr;37(4):1081-1091

College of Veterinary Medicine, Southwest University, Chongqing 400715, China.

The enterobacterial common antigen (ECA) is a polysaccharide composed of polysaccharide repeats that are located in the outer membrane of almost all Enterobacteriaceae bacteria and has diverse biological functions. ECA is synthesized by the synergistic action of multiple genes that are present in clusters on the genome of Enterobacteriaceae bacteria, forming the ECA antigen gene cluster, an important virulence factor that plays a role in host invasion and survival of Enterobacteriaceae in vivo. ECA also plays an important role in the maintenance of the bacterial outer membrane permeability barrier, flagella gene expression, swarming motility, and bile salts resistance. In addition, ECALPS, anchored in the core region of bacterial lipopolysaccharide, is an important surface antigen for bacteria, stimulating high levels of antibody production in the host and could be a target for vaccine research. This review summarizes ECA purification, genes involved in ECA biosynthesis, its immunological characteristics, biological functions and clinical applications.
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http://dx.doi.org/10.13345/j.cjb.200334DOI Listing
April 2021

Heterogeneous [email protected]@NF nanosheet arrays directly used as high efficiency bifunctional electrocatalyst for water decomposition.

J Colloid Interface Sci 2021 Oct 20;599:300-312. Epub 2021 Apr 20.

Key Laboratory of Eco-Environment-Related Polymer Materials of Ministry of Education, Key Laboratory of Polymer Materials of Gansu Province, College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou, Gansu 730070, PR China. Electronic address:

Developing and designing bifunctional electrocatalysts are very important for the production of hydrogen from water electrolysis. The reasonable interface modulation can effectively lead to the optimization of electronic configuration through the interface electron transfer in the heterostructures and thus resulting in the enhanced efficiency. In this work, self-supported and heterogeneous interface-rich [email protected]@NF electrocatalyst for overall water splitting was designed and prepared through a controllable step-wise hydrothermal process. Density functional theory calculations suggest that heterogeneous interface formed between NiS and FeNiS can optimize the Gibbs free energy for H* adsorption (ΔG). Benefiting from the open structure of the nanosheet arrays, the abundant heterogeneous interfaces in [email protected]@NF composite, the positive synergistic effect between NiS and FeNiS, and the good conductivity of foamed nickel (NF) substrate, the optimized [email protected]@NF nanoarray catalyst displayed excellent electrocatalytic activities, the overpotential is only 83 mV and 235 mV for hydrogen evolution reaction (HER) and oxygen evolution reaction (OER) at 10 mA cm, respectively. Importantly, an alkaline electrolyser directly using the [email protected]@NF as both the anode and cathode achieved an ultralow cell voltage of 1.46 V, accompanied by outstanding stability. The performance is better than that of most other transition-metal sulfides electrocatalysts. This work may provide a useful strategy for reasonably regulating heterogeneous interfaces to effectively improve the performance of materials, thus accelerating the practical application of transition-metal sulfides electrocatalysts for overall water splitting.
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http://dx.doi.org/10.1016/j.jcis.2021.04.004DOI Listing
October 2021

Transmission dynamics of the rubella virus circulating in China during 2010-2019: two lineage switches between genotypes 1E and 2B.

Clin Infect Dis 2021 Apr 27. Epub 2021 Apr 27.

Yunnan Provincial Center for Disease Control and Prevention, Kunming, China.

Background: To provide a better understanding of the progress on rubella control and elimination in China, a genetic analysis was conducted to examine the transmission pattern of the endemic rubella virus in China during 2010-2019.

Methods: Total 4895 strains were obtained from 29 out of the 31 provinces in mainland of China during 2010-2019. The genotyping region of the strains were amplified, determined, and assembled. Genotyping analysis and lineage division were performed by comparisons with the World Health Organization reference strains and previously reported lineage reference strains, respectively. Further phylogenetic analyses were performed to compare the genetic relationship.

Results: During 2010-2019, the domestic lineage 1E-L1 and multiple imported lineages of rubella viruses including 2B-L1, 1E-L2, and 2B-L2c were identified. Further analysis of the circulation trend of the different lineages indicated that two switches occurred among the lineages. The first shift was from lineage 1E-L1 to 2B-L1, which occurred around 2015-2016, followed by the lowest rubella incidence in 2017. The second shift was from lineage 2B-L1 to 1E-L2 and 2B-L2c, which occurred around 2018-2019, coinciding with rubella resurgence and the subsequent nationwide epidemic during 2018-2019. Insufficient genomic information worldwide made it impossible to trace the origin of the imported viruses in this study.

Conclusions: China was moving toward rubella elimination, as evidenced by the fact that previous endemic lineages were not detected. However, rubella reemerged in 2018 and 2019 due to the newly imported rubella viruses. Therefore, to realize the rubella elimination goal, joint efforts are required for all countries worldwide.
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http://dx.doi.org/10.1093/cid/ciab339DOI Listing
April 2021

Rapid Quantum Dot Nanobead-mAb Probe-Based Immunochromatographic Assay for Antibody Monitoring of Infection.

Int J Nanomedicine 2021 29;16:2477-2486. Epub 2021 Mar 29.

Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun, People's Republic of China.

Purpose: Sensitive and selective point-of-care biosensor is an urgent pursuit of serological antibody detection to control parasite pathogen. For specific, quantitative and on-site screening of infection in livestock, a quantum dot nanobead-monoclonal antibody (QB-mAb) probe-based immunochromatographic assay (ICA) was developed by introducing a competitive sandwich strategy (QB-CICA).

Methods: In the QB-CICA, QB-mAb probes competed with serum antibody for a particular epitope, followed by immunocomplexes binding to capture antibody on the test line. With the accumulation of target antibody, captured probes served as signal elements for fluorescent readout in a "turn off" mode, along with the fluorescence gradually weakened. The sensitivity and standard calibration curve of the QB-CICA were quantified using swine sera as negative control (n = 200) and artificial infected swine sera (n = 80) compared with a commercial ELISA kit. Besides, -antibody targeting test ability of the QB-CICA, instead of other parasites or viruses antibodies (n = 10), was evaluated.

Results: The QB-CICA exhibited a good linear range, a low detection limit of 189.92 ng mL and 100% selectivity that was higher than commercial ELISA kit (90%), as well as the same serological positive rate (100%) with commercial ELISA kit in different infection dose models.

Conclusion: Taking advantage of its simplicity, short response time (25 min), sensitivity and specificity, the proposed QB-CICA has potential applications for parasite-related antibody monitoring in food safety and clinical diagnosis fields.
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http://dx.doi.org/10.2147/IJN.S304845DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8018372PMC
April 2021

A combination of PD‑1/PD‑L1 inhibitors: The prospect of overcoming the weakness of tumor immunotherapy (Review).

Mol Med Rep 2021 05 24;23(5). Epub 2021 Mar 24.

Integrated Traditional Chinese and Western Medicine Laboratory, College of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, P.R. China.

Programmed cell death protein‑1 (PD‑1)/programmed death protein ligand‑1 (PD‑L1) inhibitors for treatment of a various types of cancers have revolutionized cancer immunotherapy. However, PD‑1/PD‑L1 inhibitors are associated with a low response rate and are only effective on a small number of patients with cancer. Development of an anti‑PD‑1/PD‑L1 sensitizer for improving response rate and effectiveness of immunotherapy is a challenge. The present study reviews the synergistic effects of PD‑1/PD‑L1 inhibitor with oncolytic virus, tumor vaccine, molecular targeted drugs, immunotherapy, chemotherapy, radiotherapy, intestinal flora and traditional Chinese medicine, to provide information for development of effective combination therapies.
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http://dx.doi.org/10.3892/mmr.2021.12001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7985997PMC
May 2021

O-GlcNAc / Akt pathway regulates glucose metabolism and reduces apoptosis in liver of piglets with acute cold stress.

Cryobiology 2021 06 27;100:125-132. Epub 2021 Feb 27.

National Experimental Teaching Demonstration Center of Animal Medicine Foundation, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, 163319, PR China. Electronic address:

Cold stress is one of the serious factors restricting the development of animal husbandry in cold areas. Cold exposure can easily lead to cold stress, slow growth and even death of newborn animals. O-GlcNAcylation modification can act as type of "stress receptor" and"nutrition sensor" in a variety of stress responses, however, it is not clear how O-GlcNAcylation can regulate glucose metabolism in the liver of piglets under cold stress. In this study, piglets 21 days of age were exposed to 4 °C for 4 h or 8 h in a phytotron. Serum cortisol and other stress hormones were used to assess body status to establish a cold stress piglet model. The changes of glycogen in liver were detected by PAS. FDP and PA were also measured to study the glycolysis level of liver. To characterize potential mechanisms of O-GlcNAcylation on the livers of cold stress piglets, AKT, GSK3β, GS, PFKFB2, AS160 and their corresponding phosphorylation were determined by Western blotting. Results show O-GlcNAcylation increased and apoptosis levels increased in the liver following cold exposure during excessive CORT or metabolic dysfunction. It is suggested that the acute cold exposure of piglets induced a sequential change in the level of O-GlcNAcylation, which may be one of the factors mediating liver cell apoptosis and glucose metabolism regulation by the O-GlcNAc/AKT pathway. These findings provide new insight into the mechanisms of the cold stress response, which can facilitate the development of new strategies to combat the effects of hypothermia.
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http://dx.doi.org/10.1016/j.cryobiol.2021.02.008DOI Listing
June 2021

Pentacyclic triterpene compounds from loquat leaves reduce skin inflammation and epidermal hyperplasia in psoriasis via inhibiting the Th17 cells.

Mol Immunol 2021 04 2;132:30-40. Epub 2021 Feb 2.

Institute of Human Virology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China; Key Laboratory of Tropical Disease Control (Sun Yat-Sen University), Ministry of Education, Guangzhou, China. Electronic address:

Psoriasis is a refractory inflammatory skin disease affecting 2 %-3 % of the world population, characterized by the infiltration and hyper-proliferation of inflammatory cells and aberrant differentiation of keratinocytes. Targeting the IL-23/ Th17 axis has been well recognized as a promising therapeutic strategy, as the IL-23/ Th17 signal plays a vital role in the pathology of psoriasis. Three pentacyclic triterpene compounds isolated from loquat leaves have been reported with significant inhibitory effects on RORγt transcription activity and Th17 cell differentiation, and excellent performance in preventing lupus nephritis pathogenesis. However, the potential effects of these pentacyclic triterpene compounds on psoriasis remain unknown. In this study, we demonstrated the potent therapeutic effects of these pentacyclic triterpene compounds on psoriasis. These three pentacyclic triterpene compounds significantly alleviated skin inflammation as well as aberrant keratinocyte proliferation in an imiquimod-induced mouse psoriasis model. These compounds also inhibited the infiltration of immune cells and the level of pro-inflammatory cytokine in the dermis, as well as the cells number and changed the cytokine profiling expression of Th17 cells. These compounds could reduce the amount of CD4 and CD8 T cells in local lymph node, but not in spleen, which is different from hydrocortisone, the positive control treatment. These results suggest better performance of these compounds than steroids on treating psoriasis with less side effects on the integrated immune system. In summary, our findings uncover the potent therapeutic effects of pentacyclic triterpene compounds on psoriasis, providing potential candidate compounds for drug development.
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http://dx.doi.org/10.1016/j.molimm.2021.01.019DOI Listing
April 2021

Host innate immune responses of geese infected with goose origin nephrotic astrovirus.

Microb Pathog 2021 Mar 28;152:104753. Epub 2021 Jan 28.

Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Special One, Nanhuyaoyuan, Hongshan District, Wuhan, 430064, China; Key Laboratory of Prevention and Control Agents for Animal Bacteriosis, Special 1, Nanhuyaoyuan, Hongshan District, Wuhan, 430064, China. Electronic address:

A novel goose astrovirus (GoAstV) outbreak in goslings, characterized by severe articular and visceral gout with high mortality, occurred in China. Although the pathogenesis of GoAstV-infected goslings has been explored in several studies, the host-immune response remains unclear. In this study, a goose astrovirus was isolated from goslings in Xiaogan, and designated as the HBXG strain. The full-length genome of HBXG was 7170 nt. A sequence analysis and phylogenetic trees revealed HBXG belonged to the novel GoAstV. We evaluated the viral distribution systematically and estimated immune related gene expression in HBXG-infected goslings. Results showed that GoAstV replicated quickly in many tissues and the highest titer was observed in the kidney, which reached 10 copies. TLR3, RIG-I and MDA5 were involved in the host-immune response to GoAstV, and the expression of IFN types I (IFN-α, IFN-β), inflammatory cytokines (IL-8, IL-10, TNF-α), antiviral proteins (Mx, OASL, PKR) and MHC-I were also upregulated during the infection. In contrast, the expression of proinflammatory cytokines (IL-1β, IL-6) and MHC-II were inhibited at 3 dpi. This study suggests that GoAstV is highly pathogenic to goslings, causing multiple systemic infections in tissues and the host-immune response is activated early in infection. However, rapid viral replication, suppression of inflammatory cytokines (IL-1β, IL-6) and MHC-II expressions were the possible reasons why the host-immune response cannot provide enough protection against GoAstV infection. This study is the first report to illuminate the immune response in goslings infected with GoAstV and offers insight into the pathogenesis of GoAstV.
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http://dx.doi.org/10.1016/j.micpath.2021.104753DOI Listing
March 2021

Fabrication of hollow flower-like magnetic FeO/C/MnO/CN composite with enhanced photocatalytic activity.

Sci Rep 2021 Jan 28;11(1):2597. Epub 2021 Jan 28.

School of Civil Engineering, Qingdao University of Technology, Qingdao, 266033, People's Republic of China.

The serious problems of environmental pollution and energy shortage have pushed the green economy photocatalysis technology to the forefront of research. Therefore, the development of an efficient and environmentally friendly photocatalyst has become a hotpot. In this work, magnetic FeO/C/MnO/CN composite as photocatalyst was synthesized by combining in situ coating with low-temperature reassembling of CN precursors. Morphology and structure characterization showed that the composite photocatalyst has a hollow core-shell flower-like structure. In the composite, the magnetic FeO core was convenient for magnetic separation and recovery. The introduction of conductive C layer could avoid recombining photo-generated electrons and holes effectively. Ultra-thin g-CN layer could fully contact with coupled semiconductor. A Z-type heterojunction between g-CN and flower-like MnO was constructed to improve photocatalytic performance. Under the simulated visible light, 15 wt% photocatalyst exhibited 94.11% degradation efficiency in 140 min for degrading methyl orange and good recyclability in the cycle experiment.
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http://dx.doi.org/10.1038/s41598-021-81974-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7844032PMC
January 2021

An electro-Fenton process to treat waste liquor of a hyperaccumulator that contains potentially toxic elements and the COD.

Int J Phytoremediation 2021 28;23(7):715-725. Epub 2020 Nov 28.

Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, China.

Rapid and safe treatment of harvested fresh biomass of hyperaccumulators is essential for phytoremediation of metal-contaminated soils. Here, an electro-Fenton (EF) process was used to remove cadmium (Cd) and chemical oxidation demand (COD) from waste liquor from the dewatering of biomass of the hyperaccumulator after flocculation precipitation. The results showed that the order of impact of the factors on the removal rate of COD and Cd was pH > electrical current density > HO dosage. Increasing pH promoted Cd removal but hindered COD removal. As current density and HO dosage increased the removal rates of both Cd and COD initially increased and then decreased. Compared to an electrocoagulation process, the addition of HO in EF process greatly enhanced Cd and zinc (Zn) removal. Speciation analysis showed that most of the Cd and Zn in the initial liquor were organically and inorganically complexed. At optimal conditions, , pH 5, current density 15 mA cm and HO dosage 9 g L, the removal efficiencies of Cd, Zn and COD reached 99.4, 99.9 and 55.5% after 80 min of EF treatment. Electro-Fenton process can therefore be used to quickly remove trace metals from the waste liquor of the hyperaccumulator.
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http://dx.doi.org/10.1080/15226514.2020.1852390DOI Listing
May 2021

High prevalence and persistence of carbapenem and colistin resistance in livestock farm environments in China.

J Hazard Mater 2021 03 31;406:124298. Epub 2020 Oct 31.

Beijing Advance Innovation Center for Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China. Electronic address:

The global dissemination of antibiotic resistance genes (ARGs) and their role as novel contaminants has garnered significant attention. However, the prevalence of ARGs in farm environments is usually underestimated and understudied. Here, colistin resistance gene mcr-1 and extended-spectrum β-lactamase (ESBL) and carbapenemase genes, all of which pose a threat to food safety and public health, were selected to investigate ARG pollution in farm environments. Target gene prevalence ranged from 22.4% to 98.8%, with broiler feedlots showing higher contamination rates than pig and layer feedlots. Moreover, the ARGs were detected in the cooperative broiler feedlot environment from 2016 to 2019, with no significant changes in mcr-1 abundance after the 2017 ban on colistin as a growth promotor. The relative abundance of target genes was also higher in solid manure than in feces and soil. Overall, our results revealed: (1) that broiler farm environments could serve as an important reservoir of mcr-1 and ESBL- and carbapenemase-encoding genes; (2) that fecal treatment processes may affect levels of ARG contamination in the environment; and (3) that residual colistin selects for the persistence of mcr-1 in farm environments. Therefore, further investigation is needed to estimate the impact of the ban on colistin as a growth promoter.
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http://dx.doi.org/10.1016/j.jhazmat.2020.124298DOI Listing
March 2021

Identification of a novel epitope specific for Gp85 protein of avian leukosis virus subgroup K.

Vet Immunol Immunopathol 2020 Dec 20;230:110143. Epub 2020 Oct 20.

College of Animal Science, Yangtze University, No.88, Jingmi Road, Jingzhou 434025, China. Electronic address:

During the past two decades, avian leukosis virus (ALV) caused tremendous economic losses to poultry industry in China. ALV-K as a newly found subgroup in recent years, which made the control and eradication of ALV more difficult as they were originated from the recombination of different subgroups. To date, specific rapid detection methods refer to ALV-K are still missing. Gp85 is the main structural protein of the virus, which mediates the invasion of host cells by the virus and determinates the classification of subgroups. In this study, we prepared a monoclonal antibody (Mab) named Km3 against Gp85 of ALV-K. Immunofluorescence assay showed that Km3 specifically recognized the strains of ALV-K rather than the strains of ALV-A or ALV-J. To explain the subgroups specificity of Km3, the epitope cognized by the Mab was identified by Western blotting using 15 overlapping fragments spanning the Gp85. Finally, the peptide AFGPRSIDTLSDWSRPQ was identified as the minimal linear epitope recognized by Km3. Alignment of Gp85 from different subgroups showed that the epitope was highly conserved among ALV-K strains, which was quite different from that of the strains from ALV -A, -B and -J. In conclusion, the Mab Km3 may serve as a useful reagent for ALV-K detection and diagnosis in the future.
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http://dx.doi.org/10.1016/j.vetimm.2020.110143DOI Listing
December 2020

Global impact of -positive bacteria on "one health".

Crit Rev Microbiol 2020 Sep 12;46(5):565-577. Epub 2020 Oct 12.

Beijing Advance Innovation Center for Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing, China, P.R. China.

Polymyxins, especially polymyxin B and polymyxin E (colistin), are considered to be the last line of defence against infections caused by multi-drug-resistant (MDR) gram-negative bacteria such as carbapenem-resistant (CRE). However, the recent emergence and dissemination of the plasmid-mediated colistin resistance gene and its variants pose a serious challenge to public health and the livestock industry. This review describes the prevalence and dissemination of -positive isolates from different sources, including animals (food animals, pet animals and wildlife), humans (healthy populations and patients) and the environment (farms, urban and rural communities and natural environments) based on existing epidemiological studies of and MCR-1-producing bacteria around the world. The major mechanisms of transmission across humans, animals and the environment are discussed.
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http://dx.doi.org/10.1080/1040841X.2020.1812510DOI Listing
September 2020

Gold nanorods-based multicolor immunosensor for visual detection of enterovirus 71 infection.

Mikrochim Acta 2020 09 10;187(10):556. Epub 2020 Sep 10.

Key Laboratory of Zoonoses Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun, 130062, People's Republic of China.

Based on the etching of gold nanorods (GNRs) and enzyme-linked immunosorbent assay (ELISA), a multicolor immunosensor for visual detection of enterovirus 71 infection is proposed. Once the immunocomplex is formed, the horseradish peroxidase bound to the ELISA plate oxidizes 3,3',5,5'-tetramethylbenzidine (TMB) into TMB in the presence of hydrogen peroxide. Subsequently, TMB quantitatively etches GNRs to the short GNRs, leading to a blue shift of longitudinal localized surface plasmon resonance and corresponding color responses. This change is used to develop two types of cut-off standards, which respond to the human anti-enterovirus at a concentration of 71 IgM antibody. The method has been validated with clinical serum samples and showed high sensitivity and specificity . This visual immunosensor has an important application value for point-of-care detection of EV71, especially in areas lacking detection equipment. Graphical abstract.
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http://dx.doi.org/10.1007/s00604-020-04534-xDOI Listing
September 2020

Extreme risk spillover between chinese and global crude oil futures.

Financ Res Lett 2020 Aug 30:101743. Epub 2020 Aug 30.

Institutes of Science and Development, Chinese Academy of Sciences, Beijing 100190, China.

This paper investigates the risk spillover between China's crude oil futures and international crude oil futures by constructing upside and downside VaR connectedness networks. The findings show that China's crude oil futures behave as a net risk receiver in the global crude oil system, in which Brent and WTI play the leading roles in risk transmission in the system. The dynamic results indicate that the risk spillover between Chinese and international crude oil futures presents obvious time-varying characteristics and has risen sharply since the beginning of 2020, induced by the COVID-19 pandemic.
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http://dx.doi.org/10.1016/j.frl.2020.101743DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7456448PMC
August 2020

Organic Multiferroic Magnetoelastic Complexes.

Adv Mater 2020 Oct 2;32(40):e2003293. Epub 2020 Sep 2.

School of Physics, State Key Laboratory of Crystal Materials, Shandong University, Jinan, 250100, China.

The design of crystal structures aids the discovery of interesting physical phenomena in organic crystals. In this work, the optimization of the coronene-tetracyanoquinodimethane (TCNQ) structure generates non-degenerate energy levels of spin-up and spin-down electrons after charge transfer, producing spontaneous spin polarization, leading to pronounced ferromagnetism. The deformed crystal lattice can significantly affect the saturation magnetization of organic ferromagnets to present a remarkable magnetoelastic coupling. Furthermore, the magnetic-field-induced lattice shrinkage of the ferromagnetic crystals supports a spin-lattice-interaction-dependent magnetoelastic coupling. This concept of organic magnetoelastic coupling will pave the way for the rapid mechanical control of spin polarization in organic multiferroic magnetoelastic materials.
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http://dx.doi.org/10.1002/adma.202003293DOI Listing
October 2020

The relationship between myofiber characteristics and meat quality of Chinese Qinchuan and Luxi cattle.

Anim Biosci 2021 Apr 24;34(4):743-750. Epub 2020 Jun 24.

College of Food Science and Engineering, Shandong Agricultural University, Taian, Shandong 271018, China.

Objective: The objectives of this study were to explore the expression patterns of myosin heavy chain (MyHC) genes of different skeletal muscles from Chinese cattle, and to investigate the relationship between myofiber characteristics and meat quality of M. longissimus lumborum (LL), M. psoas major (PM), and M. semimembranosus (SM) from Chinese Luxi and Qinchuan cattle.

Methods: Three major muscles including LL, PM, and SM from Chinese Luxi cattle and Chinese Qinchuan cattle were used in this study. The myofiber characteristics were measured by histochemical analysis. The MyHC isoforms expression was evaluated by real-time quantitative polymerase chain reaction. Quality traits including pH value, meat color, cooking loss, Warner-Bratzler shear force (WBSF) and sarcomere length were determined at day 5 postmortem.

Results: PM muscle had higher pH value, a* value, sarcomere length and lower WBSF value compared to LL and SM muscles (p<0.05). Numbers of type I myofiber and the relative expression of MyHC I mRNA in PM muscle were higher than those of LL and SM muscles (p<0.05). Myofiber diameter of PM muscle was lower than that of LL and SM muscles, regardless of myofiber types (p<0.05).

Conclusion: According to the stepwise linear regression analyses, tenderness was influenced by myofiber characteristics in all three examined muscles. Tenderness of beef muscles from Qinchuan and Luxi cattle could be improved by increasing numbers of type I myofiber.
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http://dx.doi.org/10.5713/ajas.20.0066DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7961283PMC
April 2021

Ubiquitin-Conjugating Enzyme 2S Enhances Viral Replication by Inhibiting Type I IFN Production through Recruiting USP15 to Deubiquitinate TBK1.

Cell Rep 2020 08;32(7):108044

State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150069, China; College of Animal Science and Technology, Yangtze University, Jingzhou 434100, China. Electronic address:

Type I interferon (IFN) plays an essential role in the host innate immune responses. Several ubiquitin-conjugating enzyme (E2) family members were reported to regulate type I IFN production and host antiviral immune responses. However, the molecular mechanisms are still not fully understood. Here, we report that UBE2S acts as a negative regulator in the type I IFN signaling pathway. Ectopic expression of UBE2S inhibits host antiviral immune responses and enhances viral replications, whereas deficiency of UBE2S enhances host antiviral immune responses and suppresses viral replications both in vitro and in vivo. Inhibition of type І IFN production by UBE2S is independent on its E2 and E3 enzymic activity. Mechanistically, UBE2S interacts with TBK1 and recruits ubiquitin-specific protease 15 (USP15) to remove Lys63 (K63)-linked polyubiquitin chains of TBK1. Our findings reveal a role of the UBE2S-USP15-TBK1 axis in the regulation of host antiviral innate immune responses.
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http://dx.doi.org/10.1016/j.celrep.2020.108044DOI Listing
August 2020

Effect of holistic nursing intervention on postoperative rehabilitation and reduction of complications of pelvic fracture patients.

Panminerva Med 2020 Aug 5. Epub 2020 Aug 5.

Department of Rehabilitation Medicine, Yantai Hospital of Traditional Chinese Medicine, Yantai, China -

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http://dx.doi.org/10.23736/S0031-0808.20.04035-5DOI Listing
August 2020

Revealing the A-Site Effect of Lead-Free A Sb Br Perovskite in Photocatalytic C(sp )-H Bond Activation.

Angew Chem Int Ed Engl 2020 Oct 13;59(41):18136-18139. Epub 2020 Aug 13.

Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China.

The lead-free halide perovskite A Sb Br is utilized as a photocatalyst for the first time for C(sp )-H bond activation. A Sb Br nanoparticles (A Sb Br NPs) with different ratios of Cs and CH NH (MA) show different photocatalytic activities for toluene oxidation and the photocatalytic performance is enhanced when increasing the amount of Cs. The octahedron distortion caused by A-site cations can change the electronic properties of X-site ions and further affect the electron transfer from toluene molecules to Br sites. After the regulation of A-site cations, the photocatalytic activity is higher with A Sb Br NPs than that with classic photocatalysts (TiO , WO , and CdS). The main active species involved in photocatalytic oxidation of toluene are photogenerated holes (h ) and superoxide anions ( O ). The octahedron distortion by A-site cations affecting photocatalytic activity remains unique and is also a step forward for understanding more about halide-perovskite-based photocatalysis. The relationship between octahedron distortion and photocatalysis can also guide the design of new photocatalytic systems involving other halide perovskites.
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http://dx.doi.org/10.1002/anie.202005495DOI Listing
October 2020

Coordination tailoring of water-labile 3D MOFs to fabricate ultrathin 2D MOF nanosheets.

Nanoscale 2020 Jun 16;12(24):12767-12772. Epub 2020 Jun 16.

State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou 350002, China.

A novel coordination tailoring top-down delamination strategy involving the controllable partial disassembly of water-labile 3D MOFs has been explored to fabricate ultrathin 2D MOF nanosheets, and it exhibits many advantages such as green, convenience and high efficiency. Accordingly, 2D ultrathin MOF nanosheets with a two-fold interpenetrated architecture have been rapidly obtained from a 3D pillar-layered MOF, and they show distinguished fluorescence responses to different solvents.
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http://dx.doi.org/10.1039/d0nr02956dDOI Listing
June 2020

Cysteamine triggered "turn-on" fluorescence sensor for total detection of fumonisin B, B and B.

Food Chem 2020 Oct 18;327:127058. Epub 2020 May 18.

College of Animal Sciences/ College of Life Science, Yangtze University, Jingzhou 434023, China. Electronic address:

A "turn-on" fluorescence sensor was developed for total detection of fumonisin B, B and B (FB, FB and FB) in maize samples. Rhodamine B isothiocyanate (RBITC) and gold nanoparticles (AuNPs) were employed as the energy donor-acceptor pairs. The intensity of restored fluorescence was inversely proportional to the concentration of the FBs. The limit of detection (LOD) of the sensor for FB was 23.80 pg/mL. The coefficient of variation (CV) was both less than 7% for intra- and inter-assay. The linear range was from 51.39 to 2125.92 pg/mL. The average recoveries of FBs from maize samples were ranged from 88.7% to 107.2%. The correlation coefficient of the results between the developed sensor and LC-MS/MS was 0.9970. To fulfill the procedure of the assay, only 46 min was needed. These results suggest that the fluorescence "turn-on" sensor has great potential applications in the analysis of FBs in maize samples.
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http://dx.doi.org/10.1016/j.foodchem.2020.127058DOI Listing
October 2020
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