Publications by authors named "Yuying Jiang"

46 Publications

Immunochemistry in the work-up of mesothelioma and its differential diagnosis and mimickers.

Diagn Cytopathol 2021 May 6;49(5):582-595. Epub 2021 Mar 6.

Department of Pathology, University Hospitals Cleveland Medical Center/Case Western Reserve University, Cleveland, Ohio, USA.

The differential diagnosis in cellular effusions with cytological atypia often includes malignant mesothelioma (MM), reactive mesothelial proliferation, and malignancies of metastatic origin, particularly carcinomas. The International Reporting System for Serous Fluid recently established guidelines for reporting MM. In conjunction with the cytomorphologic evaluation, the role of immunochemistry (IC) was emphasized as a very useful tool in the workup of serous fluids, especially with the availability of novel markers. Utilizing a panel of markers, IC allows the characterization of the cells, whether mesothelial or not, and when mesothelial origin is established, IC can frequently assist in delineating its benign or malignant nature. IC can also confirm metastatic disease, allowing the identification of the primary origin in most cases. This review summarizes the current status of IC and its role in the diagnosis of MM and its differential diagnosis in serous fluids.
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http://dx.doi.org/10.1002/dc.24720DOI Listing
May 2021

Application of the BACs-on-Beads assay for the prenatal diagnosis of chromosomal abnormalities in Quanzhou, China.

BMC Pregnancy Childbirth 2021 Jan 28;21(1):94. Epub 2021 Jan 28.

Prenatal Diagnosis Center, Quanzhou Women's and Children's Hospital, Quanzhou, Fujian, 362000, People's Republic of China.

Background: An increasing number of techniques have been used for prenatal diagnosis of genetic abnormalities. Our initial objective was to explore the value of the BACs-on-Beads (BoBs) assay for the prenatal diagnosis of aneuploidies and microdeletion/microduplication syndromes in Quanzhou, Southeast China.

Methods: A total of 1409 pregnant women with high-risk factors for chromosomal abnormalities admitted to Quanzhou Women's and Children's Hospital were enrolled in this study. BoBs assays and karyotype analyses were conducted for all subjects. Subsequently, chromosome microarray analysis (CMA) or fluorescence in situ hybridization (FISH) was performed to validate the findings.

Results: In this study, karyotype analysis and BoBs assay failed in 4 cases, and 2 cases, respectively. A total of 1403 cases were successfully analyzed, with success rates of 99.72% (1405/1409) and 99.85% (1407/1409) for karyotype analysis and Bobs assay, respectively. BoBs assay rapidly detected chromosomal aneuploidies in line with the karyotyping data. Additionally, 23 cases of microdeletions/microduplications were detected by BoBs assay but missed by karyotyping, including 22q11.2 microdeletions/microduplications, 5p15.32p15.33 microdeletion, Xp22.31 microdeletions/microduplications, Xq27.3 microdeletion, and Yp11.2 and Yq11.22q11.222 microduplication. In comparison with karyotyping, fewer mosaicisms were identified by BoBs assay. A high detection rate of chromosomal abnormalities was observed in the high-risk group during noninvasive prenatal testing (NIPT) (41.72%) and the abnormal ultrasound group (13.43%).

Conclusions: BoBs assay can be used for the rapid and efficient prenatal diagnosis of common aneuploidies and microdeletion/microduplication syndromes. Moreover, the combined use of BoBs assay and karyotyping in prenatal diagnosis may allow for a more effective detection of chromosomal abnormalities.
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http://dx.doi.org/10.1186/s12884-021-03589-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7844892PMC
January 2021

Astrocyte-derived exosomes carry microRNA-17-5p to protect neonatal rats from hypoxic-ischemic brain damage via inhibiting BNIP-2 expression.

Neurotoxicology 2021 Mar 10;83:28-39. Epub 2020 Dec 10.

Department of Abdominal Ultrasonography, The First Hospital of Jilin University, Changchun, Jilin, 130021, PR China. Electronic address:

Exosomes play critical roles in neurogenesis. This study aims to explore the mechanism of astrocyte-derived exosomes in neonatal rats with hypoxic-ischemic brain damage (HIBD). Astrocytes were collected and astrocyte-derived exosomes were isolated and identified. Neonatal rats were pre-treated with exosomes and then subjected to HIBD induction. Then the neurobehaviors, neuronal apoptosis, inflammation and oxidative stress in rat brain were measured. Differentially expressed microRNAs (miRNAs) in rat brain before and after HI procedure were analyzed. H19-7 cells were subjected to oxygen and glucose deprivation (OGD) for in vitro studies. Target relation between miR-17-5p and BNIP2 was identified. Gain- and loss-of functions of miR-17-5p and BNIP2 were conducted to identify their roles in viability, apoptosis, oxidative stress and inflammation of OGD-treated cells. Collectively, astrocyte-derived exosomes improved neurobehaviors, and reduced cerebral infarction, neuronal apoptosis, oxidative and inflammation in vivo and in vitro. The exosomes carried miR-17-5p bound to BNIP2 and negatively regulated BNIP2 expression in OGD-treated cells. Over-expression of miR-17-5p increased viability, and decreased OGD-induced apoptosis, oxidative stress and inflammation of H19-7 cells, which were reversed by over-expression of BNIP2. Taken together, the study suggested that astrocyte-derived exosomes could carry miR-17-5p to protect neonatal rats from HIBD via inhibiting BNIP-2 expression.
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http://dx.doi.org/10.1016/j.neuro.2020.12.006DOI Listing
March 2021

[Molecular genetic analysis of a child with de novo 16p11.2 microdeletion].

Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2020 Nov;37(11):1283-1286

Prenatal Diagnosis Center, Quanzhou Woman's and Children's Hospital, Quanzhou, Fujian, 362000 China.

Objective: To explore the genetic basis for a child featuring developmental delay, intelligent disability and language deficit.

Methods: Peripheral blood samples of the child and her parents were collected for routine G-banding karyotyping analysis and single nucleotide polymorphism array (SNP array) detection. Amniotic fluid was also sampled from the mother for karyotyping analysis and SNP array detection.

Results: No karyotypic abnormality was found with the child and her parents. SNP array showed that the child has carried a 761.4 kb microdeletion at 16p11.2, while her mother has carried a 444.4 kb microduplication at 15q13.3. Her father's result was negative. Further analysis showed that the 15q13.3 microduplication was inherited from her maternal grandfather who was phenotypically normal. Prenatal diagnosis showed that the fetus has inherited the15q13.3 microduplication from its mother.

Conclusion: The child has carried a de novo 16p11.2 microdeletion, which overlaps with 16p11.2 microdeletion syndrome region, in addition with similar clinical phenotypes. The 16p11.2 microdeletion probably underlies her abnormal phenotype.
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http://dx.doi.org/10.3760/cma.j.cn511374-20190805-00397DOI Listing
November 2020

Changes of Effective Connectivity in the Alpha Band Characterize Differential Processing of Audiovisual Information in Cross-Modal Selective Attention.

Neurosci Bull 2020 Sep 26;36(9):1009-1022. Epub 2020 Jul 26.

Brainnetome Center, Institute of Automation, Chinese Academy of Sciences, Beijing, 100190, China.

Cross-modal selective attention enhances the processing of sensory inputs that are most relevant to the task at hand. Such differential processing could be mediated by a swift network reconfiguration on the macroscopic level, but this remains a poorly understood process. To tackle this issue, we used a behavioral paradigm to introduce a shift of selective attention between the visual and auditory domains, and recorded scalp electroencephalographic signals from eight healthy participants. The changes in effective connectivity caused by the cross-modal attentional shift were delineated by analyzing spectral Granger Causality (GC), a metric of frequency-specific effective connectivity. Using data-driven methods of pattern-classification and feature-analysis, we found that a change in the α band (12 Hz-15 Hz) of GC is a stable feature across different individuals that can be used to decode the attentional shift. Specifically, auditory attention induces more pronounced information flow in the α band, especially from the parietal-occipital areas to the temporal-parietal areas, compared to the case of visual attention, reflecting a reconfiguration of interaction in the macroscopic brain network accompanying different processing. Our results support the role of α oscillation in organizing the information flow across spatially-separated brain areas and, thereby, mediating cross-modal selective attention.
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http://dx.doi.org/10.1007/s12264-020-00550-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7475144PMC
September 2020

Misidentification of Infection as Aspergillosis in a Patient with Chronic Renal Failure.

Case Rep Infect Dis 2020 21;2020:9727513. Epub 2020 Jun 21.

Department of Pathology, University of Toledo Medical Center, Toledo, OH 43614, USA.

Aspergillosis is a commonly diagnosed fungal infection. Histopathologic examination alone can have diagnostic pitfalls due to the overlapping of fungal morphology. We report a case of infection initially misdiagnosed as aspergillosis. The patient presented to the hospital with shortness of breath and chest and abdominal pain. Laboratory tests revealed leukocytosis and elevated serum liver enzymes, myoglobin and lipase. He died of hypotensive shock and brain abscesses despite antibiotic treatment. Autopsy revealed invasive fungal infection in the heart, thyroid, and brain with presence of 45-degree angled, branching hyphae. The initial diagnosis of aspergillosis was made; however, further molecular studies identified the organism as . This report reveals the potential pitfalls of morphologic diagnosis alone; and the necessity of other testing modalities to render an accurate diagnosis which is crucial for appropriate.
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http://dx.doi.org/10.1155/2020/9727513DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7333029PMC
June 2020

Genetic structure and insecticide resistance characteristics of fall armyworm populations invading China.

Mol Ecol Resour 2020 Nov 20;20(6):1682-1696. Epub 2020 Jul 20.

Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China.

The rapid wide-scale spread of fall armyworm (Spodoptera frugiperda) has caused serious crop losses globally. However, differences in the genetic background of subpopulations and the mechanisms of rapid adaptation behind the invasion are still not well understood. Here we report the assembly of a 390.38-Mb chromosome-level genome of fall armyworm derived from south-central Africa using Pacific Bioscience (PacBio) and Hi-C sequencing technologies, with scaffold N50 of 12.9 Mb and containing 22,260 annotated protein-coding genes. Genome-wide resequencing of 103 samples and strain identification were conducted to reveal the genetic background of fall armyworm populations in China. Analysis of genes related to pesticide- and Bacillus thuringiensis (Bt) resistance showed that the risk of fall armyworm developing resistance to conventional pesticides is very high. Laboratory bioassay results showed that insects invading China carry resistance to organophosphate and pyrethroid pesticides, but are sensitive to genetically modified maize expressing the Bt toxin Cry1Ab in field experiments. Additionally, two mitochondrial fragments were found to be inserted into the nuclear genome, with the insertion event occurring after the differentiation of the two strains. This study represents a valuable advance toward improving management strategies for fall armyworm.
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http://dx.doi.org/10.1111/1755-0998.13219DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7689805PMC
November 2020

Investigating and manipulating the molecular beam epitaxy growth kinetics of intrinsic magnetic topological insulator MnBiTewithangle-resolved photoemission spectroscopy.

J Phys Condens Matter 2020 Jun 26. Epub 2020 Jun 26.

Tsinghua University, Beijing, Beijing, CHINA.

Intrinsic magnetic topological insulator MnBiTeis the key to realizing the quantum anomalous Hall effect and other related quantum phenomena at a sufficiently high temperature for their practical electronic applications. The research progress on the novel material, however, is severely hindered by the extreme difficulty in preparing its high-quality thin films with well-controlled composition and thickness. Combining molecular beam epitaxy (MBE) andangle-resolved photoemission spectroscopy (ARPES), we have systematically studied the growth conditions and kinetics of MnBiTethin films prepared by simple co-evaporation of Mn, Bi and Te. The transition and competition between the Mn-doped BiTeand MnBiTephases under different growth conditions have been mapped, which gives the recipe and the key principles of growing high-quality MnBiTethin films. Particularly, to obtain high quality MnBiTefilms, it is crucial to raise the growth temperature as high as allowed by the nucleation of the films to minimize density of Mn substitutional atoms on Bi sites. The ARPES data also reveal the kinetic process in the nucleation and ripening of MnBiTeislands. These results offer the essential information for designing and optimizing the MBE growth procedure of MnBiTe-like compounds to achieve the exotic topological quantum effects.
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http://dx.doi.org/10.1088/1361-648X/aba06dDOI Listing
June 2020

Fiber-interface directional coupler inscribed by femtosecond laser for refractive index measurements.

Opt Express 2020 May;28(10):14263-14270

A novel fiber-interface directional waveguide coupler was inscribed on the surface of a coreless fiber by femtosecond laser, and was successfully applied to highly sensitive refractive index (RI) measurements. The primary arm was first inscribed to couple light from a lead-in single mode fiber to the fiber interface, then back to a lead-out single mode fiber. A side arm was inscribed parallel and in close proximity to the primary arm. Light propagating in the primary arm could then be efficiently coupled into the side arm when a phase-matching condition was met, which produced a dramatic spectral dip at the coupling wavelength. The proposed device achieved a sensitivity as high as ∼8249 nm/RIU over an RI range of 1.44-1.45, due to strong evanescent fields excited in fiber-interface waveguides. The proposed in-fiber directional coupler exhibits high mechanical strength, a compact configuration, and excellent RI sensitivity. As such, it has significant potential for practical applications in biochemical sensing.
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http://dx.doi.org/10.1364/OE.390674DOI Listing
May 2020

Identification of a new β-thalassaemia variant Term CD+32(HBB: c.32A>C) in two Chinese families.

J Clin Pathol 2020 Sep 27;73(9):593-596. Epub 2020 Feb 27.

Clinical laboratory, Jinjiang City Municipal Hospital, Quanzhou city, China

Aims: β-Thalassaemia is an inherited blood disorder caused by mutations in the β-globin gene cluster. Molecular characterisation of β-thalassaemia is essential for its diagnosis and management. More and more rare and novel mutations have been reported.

Methods: Two Chinese families with β-thalassaemia from Fujian Province were recruited in this study. The phenotypes of the probands were confirmed through haematological analysis. Routine molecular analysis of thalassaemia was employed to identify the common mutations of thalassaemia. The rare and novel mutations were detected by direct DNA sequencing.

Results: In family 1, the proband, a Chinese woman aged 31 years, showed elevated level of haemoglobin A2 (HbA2). No common mutations associated with β-thalassaemia were detected, whereas a rare mutation Term CD+32(HBB: c.32A>C) was identified through DNA sequencing. Subsequent investigation of the β-thalassaemia mutation in her family showed that her mother, her brother as well as her nephew also carried this mutation. In addition, both the proband's husband and her son carrying the rare -- mutation exhibited decreased levels of MCH, MCH and HbA2. In family 2, the proband, a child aged 1 year, showed elevated level of HbA2, but had no common mutations of β-thalassaemia. The proband was identified carrying the mutation Term CD+32(HBB: c.32A>C), which was inherited from his mother.

Conclusions: In this study, we first report a rare β-thalassaemia mutation in Fujian Province, Southeast China. Moreover, our study also identified this rare mutation in humans. This finding has helped broaden the spectrum of β-thalassaemia mutations in our region and suggested that this rare mutation may be more prevalent in the Chinese population.
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http://dx.doi.org/10.1136/jclinpath-2020-206426DOI Listing
September 2020

[Clinical and genetic study of a child with 15q11.2 microduplication].

Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2020 Jan;37(1):64-66

Prenatal Diagnosis Center, Quanzhou Woman's and Children's Health Care Hospital, Quanzhou, Fujian 362000, China.

Objective: To explore the genetic basis of a child with developmental delay and intellectual disability.

Methods: Peripheral blood samples of the child and his parents were collected for routine G-band karyotyping analysis and single nucleotide polymorphism array (SNP array) assay. Amniotic fluid sample was collected during the next pregnancy for prenatal diagnosis.

Results: No karyotypic abnormality was found in the child and his parents. SNP array showed that the child has carried a 855.3 kb microduplication in 15q11.2. His mother carried the same duplication but had no phenotypic anomaly. No microdeletion/microduplication was found in his father. Upon prenatal diagnosis, no abnormalities was found with the chromosomal karyotype and SNP array result of the fetus.

Conclusion: 15q11.2 microduplication may result in developmental delay and intellectual disability, for which CYFIP1 may be a candidate gene. However, the duplication may increase the risk but with a low penetrance. This should attract attention during clinical consultation.
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http://dx.doi.org/10.3760/cma.j.issn.1003-9406.2020.01.017DOI Listing
January 2020

A prenatal diagnosis and genetics study of five pedigrees in the Chinese population with Xp22.31 microduplication.

Mol Cytogenet 2019 11;12:50. Epub 2019 Dec 11.

Prenatal Diagnosis Center, Quanzhou Women's and Children's Hospital, Fujian Province, People's Republic of China.

Background: Copy number variations (CNVs) can contribute to human phenotype, phenotypic diversity and disease susceptibility, while others may benign. In the current study, an attempt to investigate the pathogenicity of CNVs in chromosome Xp22.31 was explored.

Methods: G-banding and SNP-array techniques were used to analyze chromosome karyotypes and CNVs in fetuses. Parents associate with five different pedigrees possessing high risk factors in pregnancy were considered with such parameters as advanced age, high risk of serological screening and ultrasound abnormalities.

Results: The fetuses' amniotic fluid karyotypes were 46, XX and those of their parents with the five pedigrees revealed no abnormalities. Here, we noticed a series of individuals with Xp22.31 duplications ranging from 534.6 kb to 1.6 Mb. It was detected through SNP array that the fetuses in Pedigree 1 and 2 had ~ 600 kb duplications in the Xp22.31 region of their X chromosomes which contained two OMIM genes, (OMIM: 306480) and part of (OMIM: 300747). The fetuses of Pedigrees 3, 4 and 5 had 1.6 Mb duplication in the same chromosome which contained four OMIM genes: (OMIM: 306480), (OMIM: 300747), (OMIM: 300102) and (OMIM: 300229). The duplications in the fetuses of Pedigrees 1 and 5 were inherited from the non-phenotypic parents. Pedigrees 3 and 4 refused to perform parental verification. Finally, four of the five pedigrees continue towards pregnancy with no abnormalities being observed during followed-ups.

Conclusion: Our study first showed duplications of Xp22.31 in Chinese population. Clinical and genetic investigation on five different pedigrees, we consider the duplication of these fragments as likely benign copy number variants (CNVs). We suggest that the duplications of Xp22.31 with recurrent duplication as a benign CNVs .
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http://dx.doi.org/10.1186/s13039-019-0461-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6907354PMC
December 2019

Molecular analysis of α-thalassemia and β-thalassemia in Quanzhou region Southeast China.

J Clin Pathol 2020 May 25;73(5):278-282. Epub 2019 Oct 25.

Prenatal Diagnosis Center, Quanzhou Women's and Children's Hospital, Quanzhou City, China.

Aims: Thalassemia is one of the most prevalent inherited disorders in south China. However, there still has no comprehensive research on molecular characterisation of α-thalassemia and β-thalassemia in the Quanzhou region of Fujian province, a city with high incidence of thalassemia in Southeast China.

Methods: A total of 11 668 cases were collected in Quanzhou region from January 2013 to June 2019. The deletions of α-thalassemia were detected by Gap-PCR, α-thalassemia and β-thalassemia mutations were detected by DNA reverse dot blot hybridisation. Rare thalassemia gene testing and DNA sequencing were performed to detect rare and novel thalassemia mutation for suspected rare thalassemia carriers.

Results: Among 11 668 subjects, 4796 (41.10%) subjects were diagnosed with thalassemia. 3298 (28.27%) subjects were α-thalassemia carriers, 26 types of α-thalassemia mutations were identified, with the common α-thalassemia genotypes being --/αα (71.47%), -α/αα (17.13%) and -α/αα (3.49%). 1407 (12.06%) subjects were β-thalassemia carriers, 18 types of β-thalassemia mutations were identified. The common five genotypes of β-thalassemia were β/β (36.53%), β/β (30.28%), β/β (17.13%), β/β (5.12%) and β/β (4.62%). Additionally, 91 (0.78%) subjects with composite α-thalassemia and β-thalassemia were identified. Furthermore, 9 α-thalassemia and β-thalassemia gene mutations (CAP +40-43 (-AAAC), IVS-I-1 (G>T), IVS-I-5 (G>C), SEA-HPFH, CD53 (-T), CD37 (A>G), -90 (C>T), CD3 (T>C), -α) were identified for the first time in the region. Among them, CD53 (-T), CD37 (A>G) and -90 (C>T) mutations were identified for the first time in Fujian province. Moreover, CD3 (T>C), -α mutations were first identified in Chinese individual.

Conclusions: Quanzhou region of South China has high incidence of thalassemia mutations. In this study, several cases of rare thalassemia mutations have been identified, providing reference for clinical consultation. The completion of this study is of great significance to strengthen the prevention and control of thalassaemia in the Quanzhou region.
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http://dx.doi.org/10.1136/jclinpath-2019-206179DOI Listing
May 2020

Quantitative analysis of wheat maltose by combined terahertz spectroscopy and imaging based on Boosting ensemble learning.

Food Chem 2020 Mar 30;307:125533. Epub 2019 Sep 30.

College of Information Science and Engineering, Henan University of Technology, Zhengzhou 450001, China; Key Laboratory of Henan Province for Grain Photoelectric Detection and Control, Henan University of Technology, Zhengzhou 450001, China. Electronic address:

To improve the prediction accuracy of existing data modeling that is based on either spectral data or image data alone, we herein propose a method for the quantitative analysis of wheat maltose contents based on the fusion of terahertz spectroscopy and terahertz imaging, which allows features and balance fusion information to be extracted from the data, and fusion modeling of the feature information to be conducted. Moreover, a Boosting-based, novel multivariate data fusion method and a Boosting iteration termination index based on the structural risk minimization theory are proposed to achieve automatic optimization of the basic model parameters of least squares support vector machines (LS-SVMs). The best results were obtained with data fusion combining spectroscopy and image feature data, with classification performances better than those obtained on single analytical sources, thereby indicating that the multivariate data fusion method proposed is an effective method for the quantitative detection of maltose content in wheat. Furthermore, four unknown maltose concentration wheat samples are analyzed quantitatively using proposed model.
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http://dx.doi.org/10.1016/j.foodchem.2019.125533DOI Listing
March 2020

Compartmentalization within Nanofibers of Double-Decker Phthalocyanine Induces High-Performance Sensing in both Aqueous Solution and the Gas Phase.

Chemistry 2019 Dec 20;25(71):16207-16213. Epub 2019 Nov 20.

Department of Chemistry, Beijing Key Laboratory for Science and Application of Functional Molecular and Crystalline Materials, University of Science and Technology Beijing, Beijing, 100083, China.

The functionalization of natural 1D architectures is dependent on hierarchically inner nanostructures. However, the artificial supramolecular nanofibers or nanotubes were rarely developed with complex inner structures. Inspired by a biomimetic strategy, single-molecule-diameter nanofibers of double-decker phthalocyanine (EuPc2) with compartmentalized internal space and fantastic electrochemical features were developed upon air/water interfacial assembly with poly-l-lysine. EuPc2/poly-l-lysine nanofibers can be electrochemical sensors both in water and the gas phase and have the best analytical performances for nitrite among all the porphyrins or phthalocyanines monomers and assemblies. Imbedding nitrite in compartments not only promotes the sensing but also changes the supramolecular chirality of nanofibers, and the morphological-dependent sensing properties of EuPc2 assemblies in water are different from that in the gas phase. These results suggest the unprecedented properties for diverse applications of artificial 1D architectures containing complex inner nanostructures.
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http://dx.doi.org/10.1002/chem.201903553DOI Listing
December 2019

Functional Supramolecular Gels Based on the Hierarchical Assembly of Porphyrins and Phthalocyanines.

Front Chem 2019 15;7:336. Epub 2019 May 15.

Beijing Key Laboratory for Science and Application of Functional Molecular and Crystalline Materials, Department of Chemistry, University of Science and Technology Beijing, Beijing, China.

Supramolecular gels containing porphyrins and phthalocyanines motifs are attracting increased interests in a wide range of research areas. Based on the supramolecular gels systems, porphyrin or phthalocyanines can form assemblies with plentiful nanostructures, dynamic, and stimuli-responsive properties. And these π-conjugated molecular building blocks also afford supramolecular gels with many new features, depending on their photochemical and electrochemical characteristics. As one of the most characteristic models, the supramolecular chirality of these soft matters was investigated. Notably, the application of supramolecular gels containing porphyrins and phthalocyanines has been developed in the field of catalysis, molecular sensing, biological imaging, drug delivery and photodynamic therapy. And some photoelectric devices were also fabricated depending on the gelation of porphyrins or phthalocyanines. This paper presents an overview of the progress achieved in this issue along with some perspectives for further advances.
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http://dx.doi.org/10.3389/fchem.2019.00336DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6530257PMC
May 2019

Molecular analysis of a large novel deletion causing α-thalassemia.

BMC Med Genet 2019 05 6;20(1):74. Epub 2019 May 6.

Prenatal Diagnosis Center, Quanzhou Woman's and Children's Hospital, No. 700 Fengze Street Fengze District, Quanzhou City, 362000, Fujian Province, People's Republic of China.

Background: α-thalassaemia is an inherited blood disorder caused by mutations in the α-globin gene cluster. Recognizing the pathogenic α-globin gene mutations associated with α-Thalassemia is of significant importance to thalassaemia's diagnosis and management.

Methods: A family with α-thalassaemia from Fujian, China was recruited for this study. The phenotype was confirmed through haematological analysis. Commercially available Gap-PCR genotypic methods were employed to identify the known deletions causing α-thalassemia. MLPA analysis was used to study the novel mutations; this was then confirmed through DNA sequencing and bioinformatics analysis.

Results: The proband of the family belonged to Southeast Asian type (--) thalassaemia. None of the known mutations associated with α-thalassaemia were detected in this family's genetics, whereas a novel 6.9 kb deletion (16p13.3 g.29,785-36,746) covering the α2 gene on the globin gene cluster was identified with MLPA and confirmed through Sanger Sequencing. This data led us to propose a novel pathogenic deletion associated with α-thalassemia: -α /--.

Conclusions: A novel α-thalassaemia deletion was identified in members of a Chinese family and subsequently analyzed. This finding has helped broaden the spectrum of pathogenic mutations leading to the development of α-thalassaemia, paving the way for improved disease diagnosis and management.
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http://dx.doi.org/10.1186/s12881-019-0797-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6501318PMC
May 2019

Preparation and characterization of low oil absorption starch via freeze-thawing.

Carbohydr Polym 2019 May 7;211:266-271. Epub 2019 Feb 7.

School of Food Science and Engineering, Jilin Agricultural University, Changchun, 130118, China. Electronic address:

The adsorption and structure of freeze-thawed (two rounds) corn (FT-CS), wheat (FT-WS), and tapioca (FT-TS) starches were studied and compared to raw samples. The decrease in total oil absorption of freeze-thawed starches was elaborated. Freeze-thawing increased the total water absorption and slightly increased matrix oil absorption with decreased amylose content. Contact angle (CA), Fourier transform infrared spectroscopy (FT-IR), and scanning electron microscopy (SEM) showed that the change in groups and the rougher surface correlated with oleophobicity. The decrease in specific surface area via particle size determination increased the oleophobicity. X-ray diffraction (XRD) indicated that all of the samples were A-type with decreased crystallinity of FT starch. This increased the matrix oil absorption. However, the increase in matrix oil absorption has little effect on the decrease of total oil absorption. In summary, freeze-thawing treatment effectively modified the starches and reduced the lipophilicity of the FT starch.
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http://dx.doi.org/10.1016/j.carbpol.2019.02.020DOI Listing
May 2019

Optimization of ultrasonic-microwave assisted alkali extraction of arabinoxylan from the corn bran using response surface methodology.

Int J Biol Macromol 2019 May 26;128:452-458. Epub 2019 Jan 26.

College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China. Electronic address:

In this study, Ultrasonic-Microwave assisted alkali (UMAA) was applied to extract water-unextractable arabinoxylan (WUAX) from the corn bran. The various factors including ultrasonic power, concentration of sodium hydroxide, solvent-to-material ratio and ultrasonic-microwave synergetic time were optimized by response surface methodology coupled with Box-Behnken design (BBD) on basis of the results of single factor experiments. The results showed that the maximum WUAX yield of 27.78 ± 0.17% was obtained with ultrasonic power 500 W, concentration of sodium hydroxide 0.30 mol/L, solvent-to-material ratio 30 mL/g, ultrasonic-microwave synergetic time 25 min. FT-IR analysis processed the characteristic peaks of polysaccharide. Chemical composition analysis indicted WUAX was mainly composed of xylose, arabinose, glucose, galactose, rhamnose, mannose, glucuronic acid and galacturonic acid, at a ratio of 38.3:28.2:22.3:5.1:2.5:0.7:1.5:1.4, respectively. In addition, the antioxidant activity assay in vitro showed that WUAX processed strong Fe chelating activity and good scavenging effect on DPPH radical. These results clearly indicated the UMAA is an effective method for extraction of antioxidant WUAX from corn bran and the polysaccharides could be exhibited broad development and application prospects.
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http://dx.doi.org/10.1016/j.ijbiomac.2019.01.138DOI Listing
May 2019

THz Spectroscopic Investigation of Wheat-Quality by Using Multi-Source Data Fusion.

Sensors (Basel) 2018 Nov 14;18(11). Epub 2018 Nov 14.

Key Laboratory of Grain Information Processing & Control, Ministry of Education, Henan University of Technology, Zhengzhou 450001, China.

In order to improve the detection accuracy for the quality of wheat, a recognition method for wheat quality using the terahertz (THz) spectrum and multi-source information fusion technology is proposed. Through a combination of the absorption and the refractive index spectra of samples of normal, germinated, moldy, and worm-eaten wheat, support vector machine (SVM) and Dempster-Shafer (DS) evidence theory with different kernel functions were used to establish a classification fusion model for the multiple optical indexes of wheat. The results showed that the recognition rate of the fusion model for wheat samples can be as high as 96%. Furthermore, this approach was compared to the regression model based on single-spectrum analysis. The results indicate that the average recognition rates of fusion models for wheat can reach 90%, and the recognition rate of the SVM radial basis function (SVM-RBF) fusion model can reach 97.5%. The preliminary results indicated that THz-TDS combined with DS evidence theory analysis was suitable for the determination of the wheat quality with better detection accuracy.
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http://dx.doi.org/10.3390/s18113945DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6263950PMC
November 2018

Multidecadal, county-level analysis of the effects of land use, Bt cotton, and weather on cotton pests in China.

Proc Natl Acad Sci U S A 2018 08 16;115(33):E7700-E7709. Epub 2018 Jul 16.

Environment and Production Technology Division, International Food Policy Research Institute, Washington, DC 20005.

Long-term changes in land use, climate, and agricultural technologies may affect pest severity and management. The influences of these major drivers can only be identified by analyzing long-term data. This study examines panel data on land use, adoption of genetically modified (Bt) insect-resistant cotton, weather, pest severity, and insecticide use on three major cotton pests for 51 counties in China during 1991-2015. Bt cotton had pervasive effects on the whole pest complex in cotton and its management. Adoption resulted in major reductions in insecticide use for bollworm control. The resulting restoration of aphid biological control decreased aphid severity. However, mirid bugs, which have few effective natural enemies in cotton, increased in severity with warming May and reduced insecticide spraying against bollworm. The effects of landscape on pest severity were pest specific. The severity of cotton aphid and mirid bugs decreased with higher land use diversity, but the severity of highly polyphagous cotton bollworm was unrelated to land use diversity. Shares of forest, water body, and unused land area were negatively associated with the severity of mirid bugs, whereas cotton bollworm responded positively to the shares of water body and unused land area. Farmers sprayed insecticides at mild infestation levels and responded aggressively to severe bollworm outbreaks. Findings support the usefulness of Bt-based plant resistance as a component of integrated pest management (IPM) but highlight the potential for unexpected outcomes resulting from agro-ecosystem feedback loops as well as the importance of climate.
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http://dx.doi.org/10.1073/pnas.1721436115DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6099863PMC
August 2018

Room temperature chiral reorganization of interfacial assembly of achiral double-decker phthalocyanine.

Phys Chem Chem Phys 2018 Mar;20(10):7223-7229

Beijing Key Laboratory for Science and Application of Functional Molecular and Crystalline Materials, Department of Chemistry, University of Science and Technology Beijing, Beijing 100083, China.

The modulation of solid-state supramolecular assemblies at room temperature is still challenging even though it potentially has very important application prospects. Herein, based on the possibility of overall conformational changes in double-decker phthalocyanine, the room temperature chiral reorganization of solid-state assemblies was investigated. LS (Langmuir-Schaefer) films of achiral double-decker cerium phthalocyanine were fabricated via air-water interfacial assembly and the dependence of supramolecular chirality on the assembly of the achiral double-decker molecules was identified. Interestingly, the corresponding supramolecular chirality in the solid-state can be reorganized with amplification of the Cotton effect and formation of helical nanostructures upon storage at room temperature and atmospheric pressure. These results open new perspectives for the regulation of supramolecular assemblies.
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http://dx.doi.org/10.1039/c7cp08647dDOI Listing
March 2018

[Analysis a family with partial Xq deletion].

Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2017 Oct;34(5):688-690

Center for Prenatal Diagnosis, Women and Children's Health Hospital and Pediatric Hospital of Quanzhou, Quanzhou, Fujian 362000, China.

Objective: To analyze partial deletion of the long arm of X chromosome in a family and explore the mechanism underlying its phenotypes.

Methods: G-banding technique was employed to analyze the karyotypes of the subjects, and fluorescence in situ hybridization (FISH) was used to analyze their X chromosomes with Xpter, Xqter and WCPX probes.

Results: The karyotypes of the proband, her mother and her fetus were all 46,X,del(X)(q24). Combined FISH and karyotyping analysis suggested that the proband and her fetus both carried a Xq24q27.3 deletion.

Conclusion: The Xq24q27.3 deletion carried by the family is closely related with premature ovarian failure but not with short stature, gonadal dysgenesis and primary amenorrhea.
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http://dx.doi.org/10.3760/cma.j.issn.1003-9406.2017.05.015DOI Listing
October 2017

Effects of Climate Change on Epidemics of Powdery Mildew in Winter Wheat in China.

Plant Dis 2017 Oct 26;101(10):1753-1760. Epub 2017 Jul 26.

State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

Powdery mildew is a highly destructive winter wheat pathogen in China. Since the causative agent is sensitive to changing weather conditions, we analyzed climatic records from regions with previous wheat powdery mildew epidemics (1970 to 2012) and investigated the long-term effects of climate change on the percent acreage (PA) of the disease. Then, using PA and the pathogen's temperature requirements, we constructed a multiregression model to predict changes in epidemics during the 2020s, 2050s, and 2080s under representative concentration pathways RCP2.6, RCP4.5, and RCP8.5. Mean monthly air temperature increased from 1970 to 2012, whereas hours of sunshine and relative humidity decreased (P < 0.001). Year-to-year temperature changes were negatively associated with those of PA during oversummering and late spring periods of disease epidemics, whereas positive relationships were noted for other periods, and year-to-year changes in relative humidity were correlated with PA changes in the early spring period of disease epidemics (P < 0.001). Our models also predicted that PA would increase less under RCP2.6 (14.43%) than under RCP4.5 (14.51%) by the 2020s but would be higher by the 2050s and 2080s and would increase least under RCP8.5 (14.37% by the 2020s). Powdery mildew will, thus, pose an even greater threat to China's winter wheat production in the future.
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http://dx.doi.org/10.1094/PDIS-02-17-0168-REDOI Listing
October 2017

Performance of different prostate specific antibodies in the cytological diagnosis of metastatic prostate adenocarcinoma.

Diagn Cytopathol 2017 Nov 9;45(11):998-1004. Epub 2017 Sep 9.

Case Western Reserve University/University Hospitals Cleveland Medical Center, Cleveland, Ohio 44106.

Background: Diagnosis of metastatic prostate adenocarcinoma (metPA) in cytology specimens can be challenging and frequently requires the use of immunohistochemistry (IHC). Prostate specific membrane antigen (PSMA) and NKX3.1 have emerged as promising IHC markers to determine prostatic origin of metPA in surgical specimens. Our goal is to evaluate the performance of PSMA and NKX3.1 and compare them with those of prostate-specific antigen (PSA) and prostate specific alkaline phosphatase (PSAP) in the cytological diagnosis of metPA MATERIALS: Cytology specimens from patients with a history of prostate adenocarcinoma at our institution between January 01, 2005 and December 31, 2015 were retrieved. IHC stains were performed on the cell blocks. In addition to the staining pattern and intensity, the sensitivity, and specificity of PSMA and NKX3.1 were assessed and compared to those of PSA and PSAP markers.

Results: A total of 56 cytology cases were retrieved with the following diagnoses: 13 metPA, 37 metastatic carcinomas from other origins, 4 rare atypical cells, and 2 benign. Additional 9 cases were re-classified as metPA based on positive PSMA and/or NKX3.1 immunostains. In our cohort of 22 cases of metPA, 18 were positive for PSMA (82%), 15 for NKX3.1 (68%), 9 for PSA (41%), and 9 for PSAP (41%). PSMA and NKX3.1 were negative in all 6 cases of metastatic carcinoma of nonprostate origin (specificity 100%). PSMA demonstrated strong membranous staining pattern, and NKX3.1 exhibited moderate nuclear staining pattern.

Conclusion: Because of their higher sensitivity and specificity, PSMA and NKX3.1 are valuable surrogate markers for metPA in cytology specimens, when compared with PSA and PSAP markers.
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http://dx.doi.org/10.1002/dc.23809DOI Listing
November 2017

Fluorescent Phthalocyanine Assembly Distinguishes Chiral Isomers of Different Types of Amino Acids and Sugars.

Langmuir 2017 07 12;33(29):7239-7247. Epub 2017 Jul 12.

Beijing Key Laboratory for Science and Application of Functional Molecular and Crystalline Materials, Department of Chemistry, University of Science and Technology Beijing , Beijing 100083, P.R. China.

The functions of some natural supramolecular architectures, such as ribosomes, are dependent on the recognition of different types of chiral biomolecules. However, the recognition of different types of chiral molecules (multiobject chiral recognition), such as amino acids and sugars, by independent and identically artificial supramolecular assembly, was rarely achieved. In this article, simple amphiphilic achiral phthalocyanine was found to form supramolecular chiral assemblies with charged water-soluble polymers upon host-guest interactions at the air/water interface. Among these systems, one identical phthalocyanine/poly(l-lysine) assembly not only can distinguish enantiomers of different amino acids but also can recognize several epimers of monose. The chiral recognitions were achieved by comparing either the steady-state fluorescence intensity or fluorescence quenching rate of phthalocyanine/poly(l-lysine) assemblies, before and after interaction with different small chiral molecules. It was demonstrated that the interactions between poly(l-lysine) and different small chiral molecules could change the aggregation of phthalocyanines. And the sensitivity of fluorescence and the excellent multiobject chiral recognition properties of the phthalocyanine/poly(l-lysine) assembly are dependent on the subtle molecular packing mode and the cooperation of different noncovalent interactions.
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http://dx.doi.org/10.1021/acs.langmuir.7b01602DOI Listing
July 2017

Hybridizing transgenic Bt cotton with non-Bt cotton counters resistance in pink bollworm.

Proc Natl Acad Sci U S A 2017 05 8;114(21):5413-5418. Epub 2017 May 8.

Department of Entomology, University of Arizona, Tucson, AZ 85721.

Extensive cultivation of crops genetically engineered to produce insecticidal proteins from the bacterium (Bt) has suppressed some major pests, reduced insecticide sprays, enhanced pest control by natural enemies, and increased grower profits. However, these benefits are being eroded by evolution of resistance in pests. We report a strategy for combating resistance by crossing transgenic Bt plants with conventional non-Bt plants and then crossing the resulting first-generation (F) hybrid progeny and sowing the second-generation (F) seeds. This strategy yields a random mixture within fields of three-quarters of plants that produce Bt toxin and one-quarter that does not. We hypothesized that the non-Bt plants in this mixture promote survival of susceptible insects, thereby delaying evolution of resistance. To test this hypothesis, we compared predictions from computer modeling with data monitoring pink bollworm () resistance to Bt toxin Cry1Ac produced by transgenic cotton in an 11-y study at 17 field sites in six provinces of China. The frequency of resistant individuals in the field increased before this strategy was widely deployed and then declined after its widespread adoption boosted the percentage of non-Bt cotton plants in the region. The correspondence between the predicted and observed outcomes implies that this strategy countered evolution of resistance. Despite the increased percentage of non-Bt cotton, suppression of pink bollworm was sustained. Unlike other resistance management tactics that require regulatory intervention, growers adopted this strategy voluntarily, apparently because of advantages that may include better performance as well as lower costs for seeds and insecticides.
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http://dx.doi.org/10.1073/pnas.1700396114DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5448178PMC
May 2017

Drying soil in North China drove the outbreak range expansion of meadow moth by facilitating long-distance migration.

Sci Rep 2016 07 25;6:30370. Epub 2016 Jul 25.

Department of Entomology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.

Studies of the mechanism underlying the range expansion of organisms have mainly focused on environmental conditions at the edges of species' distributions, potentially ignoring other possible factors. Here, we demonstrated the outbreak range expansion of meadow moth from North China to Northeast China in the past three outbreak periods. We found that the negligible infestation in Northeast China in the 1950s could not be explained by local climatic conditions. However, the soil moisture in North China decreased distinctly from 1951 to 2011 and was significantly and positively correlated with the timing of the first adult peak on plateaus, meaning that the deterioration of habitat conditions could result in earlier peaks of adults in areas of high-elevation by stimulating the short-distance dispersal of adults from the plains to the plateaus. Adults peaking earlier have a stronger tendency to emigrate due to mismatched phenology. Hence, drying soil in North China caused the frequent long-distance migration of meadow moth after the 1970s and drove the outbreak range expansion. This study suggests that, for a migratory species, the deterioration of habitat conditions in overwintering areas might also influence the distribution of this species in breeding areas at high latitudes by facilitating migration activities.
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http://dx.doi.org/10.1038/srep30370DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4959011PMC
July 2016

Quantitative determination of aflatoxin B1 concentration in acetonitrile by chemometric methods using terahertz spectroscopy.

Food Chem 2016 Oct 20;209:286-92. Epub 2016 Apr 20.

State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100080, China.

Aflatoxins contaminate and colonize agricultural products, such as grain, and thereby potentially cause human liver carcinoma. Detection via conventional methods has proven to be time-consuming and complex. In this paper, the terahertz (THz) spectra of aflatoxin B1 in acetonitrile solutions with concentration ranges of 1-50μg/ml and 1-50μg/l are obtained and analyzed for the frequency range of 0.4-1.6THz. Linear and nonlinear regression models are constructed to relate the absorption spectra and the concentrations of 160 samples using the partial least squares (PLS), principal component regression (PCR), support vector machine (SVM), and PCA-SVM methods. Our results indicate that PLS and PCR models are more accurate for the concentration range of 1-50μg/ml, whereas SVM and PCA-SVM are more accurate for the concentration range of 1-50μg/l. Furthermore, ten unknown concentration samples extracted from mildewed maize are analyzed quantitatively using these methods.
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http://dx.doi.org/10.1016/j.foodchem.2016.04.070DOI Listing
October 2016