Publications by authors named "Yuyao Li"

35 Publications

The Genetic Architecture of Grain Yield in Spring Wheat Based on Genome-Wide Association Study.

Front Genet 2021 15;12:728472. Epub 2021 Nov 15.

Heilongjiang Bayi Agricultural University, Daqing, China.

Uncovering the genetic architecture for grain yield (GY)-related traits is important for wheat breeding. To detect stable loci for GY-related traits, a genome-wide association study (GWAS) was conducted in a diverse panel, which included 251 elite spring wheat accessions mainly from the Northeast of China. In total, 52,503 single nucleotide polymorphisms (SNPs) from the wheat 55 K SNP arrays were used. Thirty-eight loci for GY-related traits were detected and each explained 6.5-16.7% of the phenotypic variations among which 12 are at similar locations with the known genes or quantitative trait loci and 26 are likely to be new. Furthermore, six genes possibly involved in cell division, signal transduction, and plant development are candidate genes for GY-related traits. This study provides new insights into the genetic architecture of GY and the significantly associated SNPs and accessions with a larger number of favorable alleles could be used to further enhance GY in breeding.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fgene.2021.728472DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8634730PMC
November 2021

The amputation and mortality of inpatients with diabetic foot ulceration in the COVID-19 pandemic and postpandemic era: A machine learning study.

Int Wound J 2021 Nov 24. Epub 2021 Nov 24.

Department of Endocrinology, School of Medicine, Bioengineering College, Chongqing Emergency Medical Center, Chongqing University Central Hospital, Chongqing University, Chongqing, China.

This study aimed to explore the clinical characteristic and outcomes of inpatients with diabetic foot ulceration (DFU) in 2019 (prelockdown) and 2020 (postlockdown) due to the COVID-19 pandemic, at an emergency medical service unit. Prediction models for mortality and amputation were developed to describe the risk factors using a machine learning-based approach. Hospitalized DFU patients (N = 23) were recruited after the lockdown in 2020 and matched with corresponding inpatients (N = 23) before lockdown in 2019. Six widely used machine learning models were built and internally validated using 3-fold cross-validation to predict the risk of amputation and death in DFU inpatients under the COVID-19 pandemic. Previous DF ulcers, prehospital delay, and mortality were significantly higher in 2020 compared to 2019. Diabetic foot patients in 2020 had higher hs-CRP levels (P = .037) but lower hemoglobin levels (P = .017). The extreme gradient boosting (XGBoost) performed best in all models for predicting amputation and mortality with the highest area under the curve (0.86 and 0.94), accuracy (0.80 and 0.90), sensitivity (0.67 and 1.00), and negative predictive value (0.86 and 1.00). A long delay in admission and a higher risk of mortality was observed in patients with DFU who attended the emergency center during the COVID-19 post lockdown. The XGBoost model can provide evidence-based risk information for patients with DFU regarding their amputation and mortality. The prediction models would benefit DFU patients during the COVID-19 pandemic.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/iwj.13723DOI Listing
November 2021

Regioselective Difluoromethane sulfonylation and Triflylation of Resorufin Derivatives.

Org Lett 2021 Nov 18;23(21):8477-8481. Epub 2021 Oct 18.

State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of New Drug Design, Frontiers Science Center for Materiobiology and Dynamic Chemistry, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China.

Reported herein is a regioselective difluoromethane sulfonylation or triflylation of resorufin derivatives, which allows easy access to 2-difluoromethane sulfonylated or triflylated resorufin derivatives in good yields. The installation of a difluoromethane sulfonyl group significantly increases the solubility of the chromophore and expands its Stokes shift. A difluoromethane sulfonylated resorufin-based fluorogenic probe proved to be able to image enzyme activity in live cells with a stronger fluorescence signal compared with its resorufin counterpart.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.orglett.1c03192DOI Listing
November 2021

Time in range in relation to amputation and all-cause mortality in hospitalised patients with diabetic foot ulcers.

Diabetes Metab Res Rev 2021 Sep 29:e3498. Epub 2021 Sep 29.

Department of Endocrinology, College of Medicine, College of Bioengineering, Chongqing Emergency Medical Center, Chongqing University Central Hospital, Chongqing University, Chongqing, China.

Aims: The aim of this study was to evaluate the association of time in range (TIR) with amputation and all-cause mortality in hospitalised patients with diabetic foot ulcers (DFUs).

Materials And Methods: A retrospective analysis was performed on 303 hospitalised patients with DFUs. During hospitalisation, TIR, mean blood glucose (MBG), coefficient of variation (CV), time above range (TAR) and time below range (TBR) of patients were determined from seven-point blood glucose profiles. Participants were grouped based on their clinical outcomes (i.e., amputation and death). Logistic regression was employed to analyse the association of TIR with amputation and all-cause mortality of inpatients with DFUs.

Results: Among the 303 enrolled patients, 50 (16.5%) had undergone amputation whereas seven (2.3%) were deceased. Blood glucose was determined in 41,012 samples obtained from all participants. Patients who underwent amputation had significantly lower TIR and higher MBG, CV, level 2 TAR and level 1 TBR whereas deceased patients had significantly lower TIR and higher MBG and level 2 TAR. Both amputation and all-cause mortality rate declined with an increase in TIR quartiles. Logistic regression showed association of TIR with amputation (p = 0.034) and all-cause mortality (p = 0.013) after controlling for 15 confounders. This association was similarly significant in all-cause mortality after further adjustment for CV (p = 0.022) and level 1 TBR (p = 0.021), respectively.

Conclusions: TIR is inversely associated with amputation and all-cause mortality of hospitalised patients with DFUs. Further prospective studies are warranted to establish a causal relationship between TIR and clinical outcomes in patients with DFUs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/dmrr.3498DOI Listing
September 2021

An explainable machine learning model for predicting in-hospital amputation rate of patients with diabetic foot ulcer.

Int Wound J 2021 Sep 14. Epub 2021 Sep 14.

Department of Endocrinology and Metabolism, Chongqing Emergency Medical Center, Chongqing Key Laboratory of Emergency Medicine, Chongqing University Central Hospital, Chongqing University, Chongqing, China.

Diabetic foot ulcer (DFU) is one of the most serious and alarming diabetic complications, which often leads to high amputation rates in diabetic patients. Machine learning is a part of the field of artificial intelligence, which can automatically learn models from data and better inform clinical decision-making. We aimed to develop an accurate and explainable prediction model to estimate the risk of in-hospital amputation in patients with DFU. A total of 618 hospitalised patients with DFU were included in this study. The patients were divided into non-amputation, minor amputation or major amputation group. Light Gradient Boosting Machine (LightGBM) and 5-fold cross-validation tools were used to construct a multi-class classification model to predict the three outcomes of interest. In addition, we used the SHapley Additive exPlanations (SHAP) algorithm to interpret the predictions of the model. Our area under the receiver-operating-characteristic curve (AUC) demonstrated a 0.90, 0.85 and 0.86 predictive ability for non-amputation, minor amputation and major amputation outcomes, respectively. Taken together, our data demonstrated that the developed explainable machine learning model provided accurate estimates of the amputation rate in patients with DFU during hospitalisation. Besides, the model could inform individualised analyses of the patients' risk factors.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/iwj.13691DOI Listing
September 2021

L. Attenuates Cigarette Smoke and Lipopolysaccharide-Induced COPD in Rats via Inflammation Inhibition and Antioxidant and Antifibrosis Pathways.

Evid Based Complement Alternat Med 2021 2;2021:6103158. Epub 2021 Mar 2.

Key Laboratory of Xinjiang Phytomedicine Resource and Utilization, Ministry of Education, College of Pharmacy, Shihezi University, Shihezi 832002, China.

The root cause behind the development of chronic obstructive pulmonary disease (COPD) is cigarette smoke that induces the inflammation of the lung tissue and alveolar destruction. Long-term cigarette smoking can lead to deterioration in lung parenchymal function and cause structural changes in the lung, further resulting in pulmonary fibrosis. L., a traditional medicinal perennial herb, is well known for its numerous pharmacological benefits, including anti-inflammation, antioxidant, antifatigue, antidepressive, and antifibrotic properties. Here, we evaluated the pharmacological effects and mechanisms of the L. (RRL) macroporous resin extract on COPD caused by lipopolysaccharide (LPS) and cigarette smoke (CS) in rats. The RRL significantly improved the pathological structure of the lung tissue. Additionally, RRL decreased the infiltration of inflammatory cells and, subsequently, oxidative stress. Furthermore, the RNAseq assay indicated that RRL attenuated the CS and LPS-induced COPD via anti-inflammatory, antifibrotic, and antiapoptotic activities. Western blot analysis substantiated that the RRL resulted in upregulated levels of Nrf2 and HO-1 as well as downregulated levels of IB, NF-B p65, -SMA, and TGF-1. Interestingly, the RRL could protect rats from CS and LPS-induced COPD by inhibiting the ERK1/2 and Smad3 signaling pathways and apoptosis. Thus, the RRL could attenuate CS and LPS-induced COPD through inflammation inhibition and antioxidant and antifibrosis pathways.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2021/6103158DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7943302PMC
March 2021

In Vivo Visualization of γ-Glutamyl Transpeptidase Activity with an Activatable Self-Immobilizing Near-Infrared Probe.

Anal Chem 2020 11 3;92(22):15017-15024. Epub 2020 Nov 3.

State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of New Drug Design, Frontiers Science Center for Materiobiology and Dynamic Chemistry, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China.

γ-Glutamyl transpeptidase (GGT), a type of cell membrane-bound enzyme, is closely involved in a wide range of physiological and pathological processes, and a large number of fluorogenic probes have been developed to detect the activity of GGT. However, the use of these imaging reagents to visualize GGT activity in vivo is largely limited because of rapid diffusion and clearance of activated fluorophores. Herein, by merging quinone methide and a fluorogenic enzyme substrate, we report an activatable self-immobilizing near-infrared probe for the in vitro and in vivo imaging of GGT activity. This probe is initially fluorescently silent, but the selective activation by GGT is able to significantly increase its fluorescence intensity at 714 nm and covalently anchor activated fluorophores at the site of interest. We have shown that this probe induced a much stronger fluorescence on live GGT-overexpressing cells compared to regular fluorogenic probes and allowed wash-free and real-time imaging of enzyme activity. More importantly, the use of this probe in the imaging of GGT activity in U87MG tumor-bearing mice by i.v. administration indicates that this self-immobilizing reagent is capable of efficiently enhancing its retention at the detection target and thus leads to much improved detection sensitivity compared to regular fluorogenic probes. This study demonstrates the advantage of fluorogenic probes with activatable anchors in the noninvasive imaging of enzyme activity in highly dynamic in vivo systems.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.analchem.0c02954DOI Listing
November 2020

SQR mediates therapeutic effects of HS by targeting mitochondrial electron transport to induce mitochondrial uncoupling.

Sci Adv 2020 Aug 26;6(35):eaaz5752. Epub 2020 Aug 26.

Department of Neurology and Suzhou Clinical Research Center of Neurological Disease, the Second Affiliated Hospital of Soochow University, Soochow University, Suzhou, China.

Hydrogen sulfide (HS) is a gasotransmitter and a potential therapeutic agent. However, molecular targets relevant to its therapeutic actions remain enigmatic. Sulfide-quinone oxidoreductase (SQR) irreversibly oxidizes HS. Therefore, SQR is assumed to inhibit HS signaling. We now report that SQR-mediated oxidation of HS drives reverse electron transport (RET) at mitochondrial complex I, which, in turn, repurposes mitochondrial function to superoxide production. Unexpectedly, complex I RET, a process dependent on high mitochondrial membrane potential, induces superoxide-dependent mitochondrial uncoupling and downstream activation of adenosine monophosphate-activated protein kinase (AMPK). SQR-induced mitochondrial uncoupling is separated from the inhibition of mitochondrial complex IV by HS. Moreover, deletion of SQR, complex I, or AMPK abolishes therapeutic effects of HS following intracerebral hemorrhage. To conclude, SQR mediates HS signaling and therapeutic effects by targeting mitochondrial electron transport to induce mitochondrial uncoupling. Moreover, SQR is a previously unrecognized target for developing non-protonophore uncouplers with broad clinical implications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1126/sciadv.aaz5752DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7449675PMC
August 2020

A self-immobilizing near-infrared fluorogenic probe for sensitive imaging of extracellular enzyme activity .

Chem Sci 2020 Jun 13;11(23):5889-5894. Epub 2020 May 13.

State Key Laboratory of Bioreactor Engineering , Shanghai Key Laboratory of New Drug Design , School of Pharmacy , East China University of Science and Technology , Shanghai 200237 , P. R. China . Email:

Reported herein is a self-immobilizing near-infrared fluorogenic probe that can be used to image extracellular enzyme activity . Using a fluorophore as a quinone methide precursor, this probe covalently anchors at sites of activation and greatly enhances the fluorescence intensity at 710 nm upon enzymatic stimulus, significantly boosting detection sensitivity in a highly dynamic system.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0sc01273dDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7449546PMC
June 2020

Physicochemical Properties of Collagen from and Its Protective Effects against HO-Induced Injury in RAW264.7 Cells.

Mar Drugs 2020 Jul 18;18(7). Epub 2020 Jul 18.

Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, 1 Haida South Road, Dinghai District, Zhoushan 316022, China.

Collagen is a promising biomaterial used in the beauty and biomedical industries. In this study, the physicochemical characterization, antioxidant activities, and protective effects against HO-induced injury of collagen isolated from were investigated. The amino acid composition analysis showed that the collagen was rich in glycine (Gly), alanine (Ala), and glutamic acid (Glu), but poor in tyrosine (Tyr) and phenylalanine (Phe). Zeta potential analysis revealed that the isoelectric point (pI) of collagen from was about 4.25. It possessed moderate scavenging activities of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radicals in a dose-dependent manner. In addition, the collagen was able to effectively improve cell viability and morphology, inhibit the production of Malondialdehyde (MDA), and increase the activities of Superoxide Dismutase (SOD) and Glutathione Peroxidase (GSH-Px) in cultured RAW264.7 cells, resulting in a protective effect against HO-induced injury. Overall, the results showed that collagen extracted from has promising prospects in the beauty and cosmetics industries.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/md18070370DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7403972PMC
July 2020

Ultrathin Cellulose Voronoi-Nanonet Membranes Enable High-Flux and Energy-Saving Water Purification.

ACS Appl Mater Interfaces 2020 Jul 6;12(28):31852-31862. Epub 2020 Jul 6.

State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Textiles, Donghua University, Shanghai 201620, China.

Creating a desirable porous membrane with high-flux and energy-saving properties for the purification of water containing submicron-sized contaminants, especially pathogenic microbes, is of great significance, yet a great challenge. Herein, we demonstrate a facile methodology to construct an innovative membrane with continuous cellulose Voronoi-nanonet structures nonsolvent-induced phase separation. This approach enables cellulose Voronoi nanonets to tightly weld with electrospun nanofibrous substrates by controlling the solvent-nonsolvent mutual diffusion process. The resultant membranes exhibit integrated properties of small pore size (0.23 μm), high porosity (90.7%), good interconnectivity, and ultrathin thickness (∼600 nm, 2 orders of magnitude thinner than the conventional microfiltration membrane). As a result, the prepared membranes can effectively intercept submicron particles (∼0.3 μm) with robust rejection efficiency (>99.80%) and ultrahigh permeation flux (maximum of 8834 L m h) under an extremely low driving pressure (≤20 kPa). More importantly, prominent bacterial rejection efficiency with a log reduction value (LRV) of 8.0 (overcoming the previous limitation of LRV <7) and outstanding antifouling function are also achieved for the membranes. The successful fabrication of such a versatile membrane may provide new insights into the development of next-generation high-performance separation materials for various applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.0c08504DOI Listing
July 2020

Ultrafine, self-crimp, and electret nano-wool for low-resistance and high-efficiency protective filter media against PM.

J Colloid Interface Sci 2020 Oct 3;578:565-573. Epub 2020 Jun 3.

Innovation Center for Textile Science and Technology, Donghua University, Shanghai 200051, China. Electronic address:

Frequent outbreaks of emerging infectious diseases (EIDs) make personal protective filter media in high demand. Electrospun nanofibrous materials are proved to be very effective in resisting virus-containing fine particles owing to their small fiber diameters; however, hindered by the intrinsic close-packing character of fine fibers, electrospun filters suffer from a relatively high air resistance, thereby poor breathing comfort. Here, we report a biomimetic and one-step strategy to create ultrafine and curly wool-like nanofibers, named nano-wool, which exhibit fluffy assembly architecture and powerful electret effect. By achieving the online self-crimp and in-situ charging of nanofibers, the curly electret nano-wool shows a small diameter of ~0.6 μm (two orders of magnitude lower than natural wool: ~20 μm) and an ultrahigh porosity of 98.7% simultaneously, together with an ultrahigh surface potential of 13260 V (one order of magnitude higher than previous filters). The structural advantages and powerful electret effect enable nano-wool to show excellent filtration efficacy (>99.995% for PM) and low air resistance (55 Pa). Additionally, nano-wool can be easily scaled up, not only holding great industrial prospect in personal protective respirators, but also paving the way for developing next-generation wool in a cost-efficient and multifunctional form.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jcis.2020.05.123DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7834036PMC
October 2020

All-polymer hybrid electret fibers for high-efficiency and low-resistance filter media.

Chem Eng J 2020 Oct 25;398:125626. Epub 2020 May 25.

State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Textiles, Donghua University, Shanghai 201620, China.

A one-step and controllable strategy to prepare all-polymer hybrid electret fibers is reported based on the coupling of polystyrene and polyvinylidene fluoride in electric response. The complementary dielectric properties between PS and PVDF generate dual-system electret charges within PS/PVDF fibers, thereby improving the electret effect. The bi-component all-polymer electret fibers show enhanced electret property and structural continuity, contributing to a N95 protective respirator with high filtration efficiency (99.752%), low air resistance (72 Pa) and long service life. The fabrication of all-polymer electret fibers solves the challenge of nanoparticle toxicity for existing polymer/nanoparticle electret fibers.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cej.2020.125626DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7255179PMC
October 2020

Stretchable and Superelastic Fibrous Sponges Tailored by "Stiff-Soft" Bicomponent Electrospun Fibers for Warmth Retention.

ACS Appl Mater Interfaces 2020 Jun 3;12(24):27562-27571. Epub 2020 Jun 3.

State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Textiles, Donghua University, Shanghai 201620, China.

Health risks in an extremely cold environment make warm retention equipment highly desirable. However, creating materials with a high warm retention performance and robust mechanical property to durably prevent against the harsh conditions is highly challenging. Herein, we report on a one-step and facile strategy to fabricate stretchable and superelastic fibrous sponges by creating unique "stiff-soft" polymer networks within fibers and bonding architecture among fibers. The premise of this design is that stiff polystyrene can endow materials with rigidity and soft polyurethane can absorb energy during mechanical deformation. Benefiting from this systematic tailoring for the polymer and assembling networks, the resultant fibrous sponges exhibit a unique tensile recovery property, a large breaking elongation of 70%, and an outstanding resilience for resisting 100 cyclic compressions with 50% strain under -50 °C. Moreover, the fibrous sponges possess dramatic characteristics of high porosity (∼99.31%), ultralight property (volume density = 7.68 mg cm), and effective warmth retention (thermal conductivity = 27.6 mW m K), as well as technical features of the simple assembly process to scale up easily. The preparation of fibrous sponges provides a new vision for developing ultralight and efficient warmth retention materials.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.0c05333DOI Listing
June 2020

In situ Synthesis of Biomimetic Silica Nanofibrous Aerogels with Temperature-Invariant Superelasticity over One Million Compressions.

Angew Chem Int Ed Engl 2020 05 3;59(21):8285-8292. Epub 2020 Mar 3.

State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Textiles, Donghua University, Shanghai, 201620, China.

Resilient and compressible three-dimensional nanomaterials comprising polymers, carbon, and metals have been prepared in diverse forms. However, the creation of thermostable elastic ceramic aerogels remains an enormous challenge. We demonstrate an in situ synthesis strategy to develop biomimetic silica nanofibrous (SNF) aerogels with superelasticity by integrating flexible electrospun silica nanofibers and rubber-like Si-O-Si bonding networks. The stable bonding structure among nanofibers is in situ constructed along with a fibrous freeze-shaping process. The resultant SNF aerogels exhibit integrated properties of ultralow density (>0.25 mg cm ), temperature-invariant superelasticity up to 1100 °C, and robust fatigue resistance over one million compressions. The ceramic nature also endows the aerogels with fire resistance and ultralow thermal conductivity. The successful synthesis of the SNF aerogels opens new pathways for the design of superelastic ceramic aerogels in a structurally adaptive and scalable form.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/anie.202001679DOI Listing
May 2020

Preparation of Lotus Root-Type Monolithic-Activated Carbons with an Hierarchical Pore Structure from Rice Husks and Their Adsorption of Vitamin B12.

ACS Omega 2019 Nov 1;4(20):18930-18935. Epub 2019 Nov 1.

School of Materials Science and Engineering and State Key Laboratory of New Ceramics and Fine Processing, Tsinghua University, Beijing 100084, PR China.

Activated carbon is widely used in many fields because of its well-developed pore structure. Especially in hemoperfusion, activated carbon beads derived from macroporous resin spheres are the predominant adsorbents in hemoditoxifiers. In comparison, biomass-activated carbon attracts more extensive attention on account of its renewability and environmental protection. In this study, a lotus root-type monolithic-activated carbon with a hierarchical pore structure was made from rice husks by the injection molding process followed by carbonization and activation. The straight square channels with the side length of about 1.3 mm were designable, and these channels with adjustable lengths were favorable for the fluid flow during blood purification compared with the tightly packed carbon beads in commercialized hemoditoxifiers. Complementally, the hierarchical nano-sized pores in the walls of the big channels would contribute much to the adsorption capacity for the monolith. Specifically, the adsorption of vitamin B12, a representative of middle molecular toxins in human blood, was about 3.7 mg g, which was acquired by simulated in vitro hemoperfusion tests and this demonstrated the promising application of the lotus root-type biomass-activated carbon in hemoperfusion.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsomega.9b03052DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6854823PMC
November 2019

Low-dose Dexamethasone Increases Autophagy in Cerebral Cortical Neurons of Juvenile Rats with Sepsis Associated Encephalopathy.

Neuroscience 2019 11 1;419:83-99. Epub 2019 Nov 1.

Department of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu 610041, China; Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu 610041, China; Department of Emergency Medicine, West China Second University Hospital, Sichuan University, Chengdu 610041, China. Electronic address:

Studies have shown that a certain dose of dexamethasone can improve the survival rate of patients with sepsis, and in sepsis associated encephalopathy (SAE), autophagy plays a regulatory role in brain function. Here, we proved for the first time that small-dose dexamethasone (SdDex) can regulate the autophagy of cerebral cortex neurons in SAE rats and plays a protective role. Cortical neurons were cultured in vitro in a septic microenvironment and a sepsis rat model was established. The small-dose dexamethasone (SdDex) or high-dose dexamethasone (HdDex) was used to intervene in neurons or SAE rats. Through fluorescence microscopy and western blot analysis, the expressions of microtubule-associated protein 1 light chain 3 (LC3), p62/sequestosome1 (p62/SQSTM1), mammalian target of rapamycin (mTOR) signaling pathway related proteins, and apoptosis-related proteins were detected. Theresultsshowthat compared with those in SAE rats, the cortical pathological changes in SAE rats treated with SdDex were improved, and damaged substances were encapsulated and degraded by autophagosomes in neurons. Additionally, similar to neurons in vitro, cortical autophagy was further activated and the mTOR signaling pathway was inhibited. After HdDex treatment, the mTOR signaling pathway in cortex is inhibited, but further activation of autophagy is not obvious, the cortical pathological changes were further worsened and the ultrastructure of neurons was disturbed. Furthermore, the HdDex group exhibited the most obvious apoptosis. SdDex can regulate autophagy of cortical neurons by inhibiting the mTOR signaling pathway and plays a protective role. Brain damage induced by HdDex may be related to the activation of apoptosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.neuroscience.2019.09.020DOI Listing
November 2019

Highly Efficient Multiple-Labeling Probes for the Visualization of Enzyme Activities.

Chemistry 2019 Nov 10;25(61):13994-14002. Epub 2019 Oct 10.

State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai, 200237, P. R. China.

Quinone methide (QM) as a latent trapping unit has been widely explored in activity-based self-immobilizing reagents. However, further application of this strategy has been largely hampered by the limited labeling efficiency to proteins. In this study, a thorough investigation on the labeling efficiency and the structure of QM-based trapping unit is presented, from which a QM with multiple leaving groups was identified as an optimal trapping unit. An alkaline phosphatase (ALP) immobilizing reagent featured with this multiple-labeling trapping unit exhibited lower nonspecific binding and, remarkably, a significantly higher labeling efficiency over other immobilizing reagents upon enzymatic activation. The utility of this imaging reagent was further demonstrated with the in vitro and in vivo visualization of the ALP activities. Furthermore, the multiple functional trapping unit may find greater value in the other activity-based immobilizing probes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/chem.201903458DOI Listing
November 2019

Role of Polymorphisms of FAM13A, PHLDB1, and CYP24A1 in Breast Cancer Risk.

Curr Mol Med 2019 ;19(8):579-588

Department of Internal Medicine Oncology, The First Affiliated Hospital of Xi'an Jiaotong University School of Medicine, Xi'an, Shaanxi 710061, China.

Background: Single-nucleotide polymorphisms (SNPs) are important indicators of susceptibility to breast cancer.

Objective: To assess the associations between SNPs in the FAM13A, PHLDB1, and CYP24A1 gene and breast cancer risk in the Chinese Han population.

Methods: We performed a case-control study including 379 female breast cancer patients and 407 female healthy controls. The three SNPs were genotyped using Agena MassARRAY platform. The χ2 test was used to compare alleles and genotypes frequencies of polymorphisms between case and control groups. Genetic models analyses to assess the associations between SNPs and breast cancer risk by computing odds ratios (ORs) and 95% confidence intervals (CIs) using logistic regression. RegulomeDB and HaploReg databases were used to calculate possible functional effects of polymorphisms.

Results: Overall analysis results showed that rs4809957 was associated with an increased risk of breast cancer (allele A: OR = 1.27, 95% CI: 1.03-1.55, p = 0.024; AA vs. GG: OR = 1.80, 95% CI: 1.15-2.82, p = 0.010; recessive model: OR = 1.70, 95% CI: 1.12-2.58, p = 0.012); and rs1059122 was found to be associated with a reduced breast cancer risk in the recessive model (OR = 0.71, 95% CI: 0.51-0.98, p = 0.039). Stratification analysis found significant associations between the three SNPs (rs1059122, rs17748, and rs4809957) and breast cancer risk.

Conclusion: Our results suggested that rs1059122 (FAM13A), rs17748 (PHLDB1), and rs4809957 (CYP24A1) might contribute to breast cancer susceptibility in the Chinese Han population. Future studies with large samples are required to confirm our findings, as well as functional studies are needed to explore their function in the breast cancer development.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2174/1566524019666190619125109DOI Listing
November 2021

Experimental and Theoretical Investigation of Laser Pretreatment on Strengthening the Heterojunction between Carbon Fiber-Reinforced Plastic and Aluminum Alloy.

ACS Appl Mater Interfaces 2019 Jun 5;11(24):22005-22014. Epub 2019 Jun 5.

Key Laboratory for Advanced Materials Processing Technology , Ministry of Education , Beijing 100084 , PR China.

Besides aluminum alloys, lightweight carbon fiber-reinforced plastics (CFRPs) have been adopted progressively in automobiles to save energy and reduce emission, so constructing a reliable heterojunction between aluminum alloys and CFRPs has come to be the key issue. In this study, ultrafast picosecond infrared (IR) and excimer ultraviolet (UV) lasers were introduced to pretreat the joint surface to enhance the adhesive strength. Scanning electron microscopy, white light interferometry, and X-ray photoelectron spectroscopy examinations indicated that because the energy absorptivities for the two lasers were different, the variation of the roughness, wettability, and chemical composition were a little different for the patterned surface. Correspondingly, the shear strengths of the adhesive joints were increased from 5.6 to 24.8 and 21.9 MPa for IR and UV laser-pretreated samples, respectively. Furthermore, finite element analysis was adopted to evaluate the effects of strengthened mechanical interlocking and fortified chemical bonding force on the enhancement of joint strength. It was shown that chemical bonding, instead of mechanical interlocking, played the dominant role in reinforcing the heterogeneous joints. As a whole, the picosecond IR laser was more preferable for surface pretreatment in adhesive heterojunctions due to its higher processing and enhancing efficiency.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.9b04080DOI Listing
June 2019

Hierarchically Mesostructured Aluminum Current Collector for Enhancing the Performance of Supercapacitors.

ACS Appl Mater Interfaces 2018 May 8;10(19):16572-16580. Epub 2018 May 8.

Beijing HCC Energy Technology Co., Ltd , Beijing 100085 , P. R. China.

Aluminum (Al) current collector is one of the most important components of supercapacitors, and its performance has vital effects on the electrochemical performance and cyclic stability of supercapacitors. In the present work, a scalable and low-cost, yet highly efficient, picosecond laser processing method of Al current collectors was developed to improve the overall performance of supercapacitors. The laser treatment resulted in hierarchical micro-nanostructures on the surface of the commercial Al foil and reduced the surface oxygen content of the foil. The electrochemical performance of the Al foil with the micro-nanosurface structures was examined in the symmetrical activated carbon-based coin supercapacitors with an organic electrolyte. The results suggest that the laser-treated Al foil (laser-Al) increased the capacitance density of supercapacitors up to 110.1 F g and promoted the rate capability due to its low contact resistance with the carbonaceous electrode and high electrical conductivity derived from its larger specific surface areas and deoxidized surface. In addition, the capacitor with the laser-Al current collector exhibited high cyclic stability with 91.5% capacitance retention after 10 000 cycles, 21.3% higher than that with pristine-Al current collector due to its stronger bonding with the carbonaceous electrode that prevented any delamination during aging. Our work has provided a new strategy for improving the electrochemical performance of supercapacitors.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.8b03647DOI Listing
May 2018

The cystathionine β-synthase/hydrogen sulfide pathway contributes to microglia-mediated neuroinflammation following cerebral ischemia.

Brain Behav Immun 2017 Nov 24;66:332-346. Epub 2017 Jul 24.

Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases and College of Pharmaceutical Sciences, Soochow University, Suzhou, China. Electronic address:

The mechanisms underlying neuroinflammation following cerebral ischemia remain unclear. Hydrogen sulfide (HS), a newly identified gasotransmitter, has been reported to regulate inflammation. In the current study, we investigated whether the endogenous HS production pathway contributed to microglia-mediated neuroinflammation following stroke. We used a mouse middle cerebral artery occlusion (MCAO) model and an in vitro cellular model to mimic ischemia-induced microglial neuroinflammation. Expression of the HS synthase cystathionine β-synthase (CBS) and HS synthetic activity were rapidly decreased in the ischemic brain tissue following MCAO. Consistently, when cultured microglia were polarized toward a pro-inflammatory phenotype with conditioned medium collected from neurons that had been subjected to oxygen-glucose deprivation (OGD neuron CM), they displayed reduced CBS expression and HS production. Enhancing HS bioavailability either by overexpressing CBS or by supplementing with exogenous HS donors promoted a shift in microglial polarization from ischemia-induced pro-inflammatory phenotypes toward anti-inflammatory phenotypes. Mechanistically, microglia that were exposed to OGD neuron CM displayed reduced activation of AMP-activated protein kinase (AMPK), which was rescued by overexpressing CBS or by supplementing with HS donors. Moreover, the promoting effects of HS donors on microglial anti-inflammatory polarization were abolished by an AMPK inhibitor or CaMKKβ inhibitor. Our results suggested that reduced CBS-HS-AMPK cascade activity contributed to microglia-mediated neuroinflammation following stroke. Targeting the CBS-HS pathway is a promising therapeutic approach for ischemic stroke.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbi.2017.07.156DOI Listing
November 2017

The F-box Protein KIB1 Mediates Brassinosteroid-Induced Inactivation and Degradation of GSK3-like Kinases in Arabidopsis.

Mol Cell 2017 Jun;66(5):648-657.e4

Department of Plant Biology, Carnegie Institution for Science, Stanford, CA 94305, USA. Electronic address:

The glycogen synthase kinase-3 (GSK3) family kinases are central cellular regulators highly conserved in all eukaryotes. In Arabidopsis, the GSK3-like kinase BIN2 phosphorylates a range of proteins to control broad developmental processes, and BIN2 is degraded through unknown mechanism upon receptor kinase-mediated brassinosteroid (BR) signaling. Here we identify KIB1 as an F-box E3 ubiquitin ligase that promotes the degradation of BIN2 while blocking its substrate access. Loss-of-function mutations of KIB1 and its homologs abolished BR-induced BIN2 degradation and caused severe BR-insensitive phenotypes. KIB1 directly interacted with BIN2 in a BR-dependent manner and promoted BIN2 ubiquitination in vitro. Expression of an F-box-truncated KIB1 caused BIN2 accumulation but dephosphorylation of its substrate BZR1 and activation of BR responses because KIB1 blocked BIN2 binding to BZR1. Our study demonstrates that KIB1 plays an essential role in BR signaling by inhibiting BIN2 through dual mechanisms of blocking substrate access and promoting degradation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.molcel.2017.05.012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5935450PMC
June 2017

Low-Resistance Dual-Purpose Air Filter Releasing Negative Ions and Effectively Capturing PM.

ACS Appl Mater Interfaces 2017 Apr 23;9(13):12054-12063. Epub 2017 Mar 23.

Key Laboratory of Textile Science & Technology, Ministry of Education, College of Textiles, Donghua University , Shanghai 201620, China.

The fatal danger of pollution due to particulate matter (PM) calls for both high-efficiency and low-resistance air purification materials, which also provide healthcare. This is however still a challenge. Herein, a low-resistance air filter capable of releasing negative ions (NIs) and efficiently capturing PM was prepared by electrospinning polyvinylidene fluoride (PVDF) fibers doped with negative ions powder (NIPs). The air-resistance of fibrous membranes decreased from 9.5 to 6 Pa (decrease of 36%) on decreasing the average fiber diameter from 1.16 to 0.41 μm. Moreover, the lower rising rate of air-resistance with reduction in pore size, for fibrous membranes with thinner fiber diameter was verified. In addition, a single PVDF/NIPs fiber was provided with strong surface potentials, due to high fluorine electronegativity, and tested using atomic force microscopy. This strong surface potential resulted in higher releasing amounts of NIs (RANIs). Interestingly, reduction of fiber diameter favored the alleviation of the shielding effects on electric field around fibers and promoted the RANIs from 798 to 1711 ions cc. Moreover, by regulating the doping contents of NIPs, the RANIs increased from 1711 to 2818 ions cc. The resultant fibrous membranes showed low air resistance of 40.5 Pa. Field-tests conducted in Shanghai showed stable PM purification efficiency of 99.99% at high RANIs, in the event of haze.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.7b00351DOI Listing
April 2017

Cleanable Air Filter Transferring Moisture and Effectively Capturing PM.

Small 2017 03 17;13(11). Epub 2017 Jan 17.

Key Laboratory of Textile Science and Technology, Ministry of Education, College of Textiles, Donghua University, Shanghai, 201620, China.

The lethal danger of particulate matter (PM) pollution on health leads to the development of challenging individual protection materials that should ideally exhibit a high PM purification efficiency, low air resistance, an important moisture-vapor transmission rate (MVTR), and an easy-to-clean property. Herein, a cleanable air filter able to rapidly transfer moisture and efficiently capture PM is designed by electrospinning superhydrophilic polyacrylonitrile/silicon-dioxide fibers as the adsorption-desorption vector for moisture-vapor, and hydrophobic polyvinylidene fluoride fibers as the repellent components to avoid the formation of capillary water under high humidity. The desorption rate of water molecules increases from 10 to 18 mg min , while the diameters of polyacrylonitrile fibers reduce from 1.02 to 0.14 µm. Significantly, by introducing the hydroxyl on the surface of polyacrylonitrile nanofibers, rapid adsorption-desorption of the water molecules is observed. Moreover, by constructing a hydrophobic to super-hydrophilic gradient structure, the MVTR increases from 10 346 to 14 066 g m d . Interestingly, the prepared fibrous membranes is easy to clean. More importantly, benefiting from enhanced slip effect, the resultant fibrous membranes presented a low air resistance of 86 Pa. A field test in Shanghai shows that the air filter maintains stable PM purification efficiency of 99.99% at high MVTR during haze event.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/smll.201603306DOI Listing
March 2017

An Investigation of the Differences and Similarities between Generated Small-World Networks for Right- and Left-Hand Motor Imageries.

Sci Rep 2016 11 4;6:36562. Epub 2016 Nov 4.

Bioimaging Core, Faculty of Health Sciences, University of Macau, Macau SAR China.

In this study, small-world network analysis was performed to identify the similarities and differences between functional brain networks for right- and left-hand motor imageries (MIs). First, Pearson correlation coefficients among the nodes within the functional brain networks from healthy subjects were calculated. Then, small-world network indicators, including the clustering coefficient, the average path length, the global efficiency, the local efficiency, the average node degree, and the small-world index, were generated for the functional brain networks during both right- and left-hand MIs. We identified large differences in the small-world network indicators between the functional networks during MI and in the random networks. More importantly, the functional brain networks underlying the right- and left-hand MIs exhibited similar small-world properties in terms of the clustering coefficient, the average path length, the global efficiency, and the local efficiency. By contrast, the right- and left-hand MI brain networks showed differences in small-world characteristics, including indicators such as the average node degree and the small-world index. Interestingly, our findings also suggested that the differences in the activity intensity and range, the average node degree, and the small-world index of brain networks between the right- and left-hand MIs were associated with the asymmetry of brain functions.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/srep36562DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5109909PMC
November 2016

Synthesis and biological evaluation of novel hydrogen sulfide releasing nicotinic acid derivatives.

Bioorg Med Chem 2016 11 30;24(21):5368-5373. Epub 2016 Aug 30.

Department of Medicinal Chemistry, College of Pharmaceutical Sciences, Soochow University, 199 Renai Road, Suzhou, Jiangsu Province 215123, China. Electronic address:

Twelve novel hybrids of slowly releasing hydrogen sulfide donor ADT-OH combined with nicotinic acid were synthesized. All of their structures had been confirmed by H NMR, C NMR and MS spectra. The target compounds were evaluated for their neuroprotective effects on hippocampal neuron HT22 cells against glutamate-induced injury at the concentrations of 1-100μM with MTT assay, and their toxicity on HT22 cells untreated by glutamine at the concentration of 100μM. The active compound was further investigated for its effect on ischemic infarct volume by intraperitoneal injection at 3h after ischemia in mice models of permanent middle cerebral artery occlusion (pMCAO). The results showed that all the compounds significantly protected HT22 cells from glutamate-induced damage at most of the experimental concentrations, and had no or little neurotoxicity on normal HT22 cells at the high concentration. More importantly, compound A6 significantly reduced infarct volume in the pMCAO model. These results suggested that compound A6 may be promising for further evaluation for the intervention of cerebral ischemic injury.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bmc.2016.08.060DOI Listing
November 2016

Illumina-based analysis of the rhizosphere microbial communities associated with healthy and wilted Lanzhou lily (Lilium davidii var. unicolor) plants grown in the field.

World J Microbiol Biotechnol 2016 Jun 27;32(6):95. Epub 2016 Apr 27.

Key Laboratory of Stress Physiology and Ecology, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou, 730000, China.

Lanzhou lily (Liliumdavidii var. unicolor) is the best edible lily as well as a traditional medicinal plant in China. The microbes associated with plant roots play crucial roles in plant growth and health. However, little is known about the differences of rhizosphere microbes between healthy and wilted Lanzhou lily (Lilium davidii var. unicolor) plants. The objective of this study was to compare the rhizosphere microbial community and functional diversity of healthy and wilted plants, and to identify potential biocontrol agents with significant effect. Paired end Illumina Mi-Seq sequencing of 16S rRNA and ITS gene amplicons was employed to study the bacterial and fungal communities in the rhizosphere soil of Lanzhou lily plants. BIOLOG technology was adopted to investigate the microbial functional diversity. Our results indicated that there were major differences in the rhizosphere microbial composition and functional diversity of wilted samples compared with healthy samples. Healthy Lanzhou lily plants exhibited lower rhizosphere-associated bacterial diversity than diseased plants, whereas fungi exhibited the opposite trend. The dominant phyla in both the healthy and wilted samples were Proteobacteria and Ascomycota, i.e., 34.45 and 64.01 %, respectively. The microbial functional diversity was suppressed in wilted soil samples. Besides Fusarium, the higher relative abundances of Rhizoctonia, Verticillium, Penicillium, and Ilyonectria (Neonectria) in the wilted samples suggest they may pathogenetic root rot fungi. The high relative abundances of Bacillus in Firmicutes in healthy samples may have significant roles as biological control agents against soilborne pathogens. This is the first study to find evidence of major differences between the microbial communities in the rhizospheric soil of healthy and wilted Lanzhou lily, which may be linked to the health status of plants.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11274-016-2051-2DOI Listing
June 2016

The new flagella-associated collagen-like proteins ClpB and ClpC of Bacillus amyloliquefaciens FZB42 are involved in bacterial motility.

Microbiol Res 2016 Mar 23;184:25-31. Epub 2015 Dec 23.

Gaolan Station of Agricultural and Ecological Experiment, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou, China; Key Laboratory of Stress Physiology and Ecology in Cold and Arid Regions of Gansu Province, Lanzhou, China.

Collagen-like proteins (CLPs) share the distinctive Gly-X-Thr repeating amino acid sequence of animal collagens, and contain N- and C-terminal domain making a collagen-like structure in Bacillus amyloliquefaciens FZB42, a plant growth-promoting rhizobacterium. Our previous study demonstrated that CLPs play important roles in biofilm construction and adherence to the surfaces on plant roots. However, bacterial localization of the CLPs remains unclear. Here, disrupted strains on all four clp genes (clpA, clpB, clpC and clpD) shown fewer filament than wild-type bacteria in extracellular matrix under scanning electron microscope (SEM). Transmission electron microscopy (TEM) was used to observe the differences on filament which associated on the cell surface, then the CLPs mutation strains showed less flagella than the wild type. Immunogold labeling determined the location that ClpB and ClpC localized on the flagella surface. In addition, western blotting analysis of crude flagella extracts suggested that the ClpB and ClpC are associated to flagella as well. The mutation strains also reduced motility of swimming on the surface of soft agar medium and changed the architectural of microcolony biofilm edge. The study suggests that collagen-like protein ClpB and ClpC, as novel proteins, associated with flagella in B. amyloliquefaciens.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.micres.2015.12.004DOI Listing
March 2016

The 5-Lipoxygenase Inhibitor Zileuton Confers Neuroprotection against Glutamate Oxidative Damage by Inhibiting Ferroptosis.

Biol Pharm Bull 2015 ;38(8):1234-9

Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases and College of Pharmaceutical Sciences, Soochow University.

5-Lipoxygenase (5-LOX) inhibitors have been shown to be protective in several neurodegenerative disease models; however, the underlying mechanisms remain unclear. We investigated whether 5-LOX inhibitor zileuton conferred direct neuroprotection against glutamate oxidative toxicity by inhibiting ferroptosis, a newly identified iron-dependent programmed cell death. Treatment of HT22 mouse neuronal cell line with glutamate resulted in significant cell death, which was inhibited by zileuton in a dose-dependent manner. Consistently, zileuton decreased glutamate-induced production of reactive oxygen species but did not restore glutamate-induced depletion of glutathione. Moreover, the pan-caspase inhibitor Z-Val-Ala-Asp(OMe)-fluoromethyl ketone (ZVAD-fmk) neither prevented HT22 cell death induced by glutamate nor affected zileuton protection against glutamate oxidative toxicity, suggesting that zileuton did not confer neuroprotection by inhibiting caspase-dependent apoptosis. Interestingly, glutamate-induced HT22 cell death was significantly inhibited by the ferroptosis inhibitor ferrostatin-1. Moreover, zileuton protected HT22 neuronal cells from erastin-induced ferroptosis. However, we did not observe synergic protective effects of zileuton and ferrostatin-1 on glutamate-induced cell death. These results suggested that both the 5-LOX inhibitor zileuton and the ferropotosis inhibitor ferrostatin-1 acted through the same cascade to protect against glutamate oxidative toxicity. In conclusion, our results suggested that zileuton protected neurons from glutamate-induced oxidative stress at least in part by inhibiting ferroptosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1248/bpb.b15-00048DOI Listing
May 2016
-->