Publications by authors named "Yuxin Zhao"

86 Publications

Expanding the scope of plant genome engineering with Cas12a orthologs and highly multiplexable editing systems.

Nat Commun 2021 03 29;12(1):1944. Epub 2021 Mar 29.

Department of Plant Science and Landscape Architecture, University of Maryland, College Park, MD, USA.

CRISPR-Cas12a is a promising genome editing system for targeting AT-rich genomic regions. Comprehensive genome engineering requires simultaneous targeting of multiple genes at defined locations. Here, to expand the targeting scope of Cas12a, we screen nine Cas12a orthologs that have not been demonstrated in plants, and identify six, ErCas12a, Lb5Cas12a, BsCas12a, Mb2Cas12a, TsCas12a and MbCas12a, that possess high editing activity in rice. Among them, Mb2Cas12a stands out with high editing efficiency and tolerance to low temperature. An engineered Mb2Cas12a-RVRR variant enables editing with more relaxed PAM requirements in rice, yielding two times higher genome coverage than the wild type SpCas9. To enable large-scale genome engineering, we compare 12 multiplexed Cas12a systems and identify a potent system that exhibits nearly 100% biallelic editing efficiency with the ability to target as many as 16 sites in rice. This is the highest level of multiplex edits in plants to date using Cas12a. Two compact single transcript unit CRISPR-Cas12a interference systems are also developed for multi-gene repression in rice and Arabidopsis. This study greatly expands the targeting scope of Cas12a for crop genome engineering.
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http://dx.doi.org/10.1038/s41467-021-22330-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8007695PMC
March 2021

Comorbidities' potential impacts on severe and non-severe patients with COVID-19: A systematic review and meta-analysis.

Medicine (Baltimore) 2021 Mar;100(12):e24971

Department of Social Medicine and Health Management, Xiangya School of Public Health, Central South University, Changsha, Hunan Province.

Background: An ongoing outbreak of pneumonia associated with the severe acute respiratory coronavirus (SARS-CoV-2) emerged in December 2019 in Wuhan, China. Epidemiologic evidence suggests that patients with comorbidities and novel coronavirus disease 2019 (COVID-19) infection may have poor survival outcomes. However, the risk of these coexisting medical conditions in severe and non-severe cases has not been systematically reported.

Purpose: The present study aimed to estimate the association of chronic comorbidities in severe and non-severe cases.

Methods: A literature search was conducted using the databases PubMed, Embase, China National Knowledge Infrastructure (CNKI), and Wanfang Database, Chinese Scientific Journals Full-text Database (CQVIP) from the inception dates to April 1, 2020, to identify cohort studies assessing comorbidity and risk of adverse outcome. Either a fixed- or random-effects model was used to calculate the overall combined risk estimates.

Results: A total of 22 studies involving 3286 patients with laboratory-confirmed COVID-19 were included in the analysis. Overall, compared with the patients with non-severe cases, the pooled odds ratios (ORs) of hypertension, diabetes mellitus, and cardiovascular, cerebrovascular, and respiratory diseases in patients with severe cases were 2.79 (95% confidence intervals [95% CI]: 1.66-4.69), 1.64 (95% CI: 2.30-1.08), 1.79 (95% CI: 1.08-2.96), 3.92 (95% CI: 2.45-6.28), and 1.98 (95% CI: 1.26-3.12), respectively.

Conclusions: This meta-analysis supports the finding that chronic comorbidities may contribute to severe outcome in patients with COVID-19. According to the findings of the present study, old age and 2 or more comorbidities are significantly impactful to COVID-19 outcomes in hospitalized patients in China.
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http://dx.doi.org/10.1097/MD.0000000000024971DOI Listing
March 2021

Pillararene-based self-assemblies for electrochemical biosensors.

Biosens Bioelectron 2021 Jun 13;181:113164. Epub 2021 Mar 13.

Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, 637371, Singapore. Electronic address:

The ingenious design and synthesis of novel macrocycles bring out renewed vigor of supramolecular chemistry in the past decade. As an intriguing class of macrocycles, pillararene and pillararene-based functional materials that are constructed through the noncovalent bond self-assembly approach have been undergoing a rapid growth, benefiting from their unique structures and physiochemical properties. This review elaborates recent significant advances of electrochemical studies based on pillararene systems. Fundamental electrochemical behavior of pillar[n]arene[m]quinone and pillararene-based self-assemblies as well as their applications in electrochemical biosensors are highlighted. In addition, the advantages and functions of pillararene self-assembly systems resulted from the unique molecular architectures are analyzed. Finally, current challenges and future development tendency in this burgeoning field are discussed from the viewpoint of both fundamental research and applications. Overall, this review not only manifests the main development vein of pillararene-based electrochemical systems, but also conquers a solid foundation for their further bioelectrochemical applications.
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http://dx.doi.org/10.1016/j.bios.2021.113164DOI Listing
June 2021

Antimicrobial photodynamic therapy versus antibiotics as an adjunct in the treatment of periodontitis and peri-implantitis: A systematic review and meta-analysis.

Photodiagnosis Photodyn Ther 2021 Feb 20;34:102231. Epub 2021 Feb 20.

The Affiliated Hospital of Stomatology, School of Stomatology, Zhejiang University School of Medicine, and Key Laboratory of Oral Biomedical Research of Zhejiang Province, Hangzhou, Zhejiang, 310006, China. Electronic address:

Background: Clinical efficacy of antimicrobial photodynamic therapy (aPDT) as compared to antibiotics in periodontitis and peri-implantitis has been tested in several clinical trials. Yet controversial results were reported. The aim of the present study was to answer the question: "Will adjunctive antimicrobial photodynamic therapy be more effective than antibiotics agent in the treatment of periodontitis and peri-implantitis?".

Methods: Publications compared outcomes between aPDT and antibiotics in adult patients with periodontitis or peri-implantitis, containing more than 3-month follow-up duration, were involved in the systematic review and meta-analysis. PubMed, EMBASE and Cochrane Central were searched until December of 2020. Clinical parameters including pocket probing depth (PPD), clinical attachment level (CAL), and bleeding on probing (BOP) were evaluated. The risk of bias was assessed by Cochrane Collaboration Tool. Weighted mean differences (WMD), 95 % confidence interval(CI) and heterogeneity were estimated by Review Manager software.

Results: 10 trials in periodontitis and 5 trials in peri-implantitis were included. Meta-analysis outcomes revealed equal clinical evidence for aPDT and antibiotics in periodontitis and peri-implantitis. In addition, aPDT significantly reduced the red complex in both diseases. However, owing to the heterogeneity of protocols in articles and the limited number of studies, the comparative conclusion remained unconfirmed.

Conclusion: aPDT can be considered as an alternative to antibiotics in the treatment of peri-implantitis and periodontitis. Given that high heterogeneity in outcome was found in this review, future long-term clinical trials with standard aPDT and antibiotic treatment should be tested to arrive at a firm conclusion.
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http://dx.doi.org/10.1016/j.pdpdt.2021.102231DOI Listing
February 2021

IL-1 Receptor Antagonist Protects the Osteogenesis Capability of Gingival-Derived Stem/Progenitor Cells under Inflammatory Microenvironment Induced by Lipopolysaccharides.

Stem Cells Int 2021 16;2021:6638575. Epub 2021 Jan 16.

The Affiliated Hospital of Stomatology, School of Stomatology, Zhejiang University School of Medicine, and Key Laboratory of Oral Biomedical Research of Zhejiang Province, Hangzhou, Zhejiang 310006, China.

Mesenchymal stem cells (MSCs) have been considered to be a future treatment option for periodontitis due to their excellent regenerative capability. However, it is still a challenge to protect MSCs' biological properties from multiple bacterial toxins in local inflammatory environment. The present study is aimed at investigating the treatment effect of interleukin-1 receptor antagonist (IL-1ra) on cell proliferation, migration, and osteogenic differentiation of gingival-derived mesenchymal stem cells (GMSCs) under an inflammatory microenvironment induced by lipopolysaccharides (LPS). GMSCs derived from Sprague-Dawley (SD) rats' free gingival tissues were treated with LPS (10 g/mL) to create inflammatory environment. Different concentrations of IL-1ra (0.01-1 g/mL) were used to antagonize the negative effect of LPS. Cell behaviors including proliferation, cloning formation unit (CFU), cell migration, osteogenic differentiation, mineral deposition, and cytokine production were assessed to investigate the protection effect of IL-1ra on GMSCs under inflammation. The toll-like receptor 4 (TLR4)/nuclear factor kappa B (NF-B) pathway activated by LPS was evaluated by real-time quantitative polymerase chain reaction (RT-PCR) and western blot. In response to LPS treatment, cell numbers, cloning formation rate, cell migration rate, proinflammatory cytokine production, and osteogenic differentiation-associated protein/mRNA expressions as well as mineralized nodules were suppressed in a time-dependent manner. These negative effects were effectively attenuated by IL-1ra administration in a time- and dose-dependent manner. In addition, mRNA expressions of TLR4 and IkB decreased dramatically when IL-1ra was added into LPS-induced medium. IL-1ra also reversed the LPS-induced TLR4/NF-B activation as indicated by western blot. The present study revealed that IL-1ra decreased inflammatory cytokine production in a supernatant, so as to protect GMSCs' osteogenesis capacity and other biological properties under LPS-induced inflammatory environment. This might be explained by IL-1ra downregulating TLR4-mediated NF-B signaling pathway activation.
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http://dx.doi.org/10.1155/2021/6638575DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7834827PMC
January 2021

Efficient deletion of multiple circle RNA loci by CRISPR-Cas9 reveals Os06circ02797 as a putative sponge for OsMIR408 in rice.

Plant Biotechnol J 2021 Jan 13. Epub 2021 Jan 13.

Department of Biotechnology, School of Life Sciences and Technology, Center for Informational Biology, University of Electronic Science and Technology of China, Chengdu, China.

CRISPR-Cas9 is an emerging genome editing tool for reverse genetics in plants. However, its application for functional study of non-coding RNAs in plants is still at its infancy. Despite being a major class of non-coding RNAs, the biological roles of circle RNAs (circRNAs) remain largely unknown in plants. Previous plant circRNA studies have focused on identification and annotation of putative circRNAs, with their functions largely uninvestigated by genetic approaches. Here, we applied a multiplexed CRISPR-Cas9 strategy to efficiently acquire individual null mutants for four circRNAs in rice. We showed each of these rice circRNA loci (Os02circ25329, Os06circ02797, Os03circ00204 and Os05circ02465) can be deleted at 10% or higher efficiency in both protoplasts and stable transgenic T0 lines. Such high efficiency deletion enabled the generation of circRNA null allele plants without the CRISPR-Cas9 transgene in the T1 generation. Characterization of the mutants reveals these circRNAs' participation in salt stress response during seed germination and in particular the Os05circ02465 null mutant showed high salt tolerance. Notably, the seedlings of the Os06circ02797 mutant showed rapid growth phenotype after seed germination with the seedlings containing higher chlorophyll A/B content. Further molecular and computational analyses suggested a circRNA-miRNA-mRNA regulatory network where Os06circ02797 functions to bind and sequester OsMIR408, an important and conserved microRNA in plants. This study not only presents genetic evidence for the first time in plants that certain circRNAs may serve as sponges to negatively regulate miRNAs, a phenomenon previously demonstrated in mammalian cells, but also provides important insights for improving agronomic traits through gene editing of circRNA loci in crops.
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http://dx.doi.org/10.1111/pbi.13544DOI Listing
January 2021

Factors associated with childbirth self-efficacy: a multicenter cross-sectional study in China.

Midwifery 2021 Feb 13;93:102883. Epub 2020 Nov 13.

School of Public Health, Tongji Medical College, Huazhong University of Science and Technology. Electronic address:

Objective: Childbirth self-efficacy is an important indicator of a woman's choice of mode of delivery, and has been found to be closely related to adverse perinatal outcomes. This study was conducted to evaluate childbirth self-efficacy and its influencing factors among pregnant Chinese women.

Design: A multicenter cross-sectional study.

Methods: Pregnant women were recruited by using a convenience sample in four maternal and child healthcare hospitals, one each from four cities in China between May and June 2018. Women with a singleton pregnancy, aged 18 years and older, and able to read and write the Chinese language were included. Trained investigators used a structured self-administrated questionnaire to collect data. EpiData 3.1 was used to prepare the database, and SAS 9.2 was used for statistical analysis. A one-way ANOVA test or independent sample t-tests were used to test the between-group differences in childbirth self-efficacy scores, as appropriate. Multivariate linear regression analyses were conducted to examine childbirth self-efficacy of the pregnant women, and the socio-demographic and obstetric factors that influence childbirth self-efficacy.

Findings: A total of 1,796 pregnant Chinese women with singleton pregnancies were recruited into this study. The average childbirth self-efficacy score of the participants was 14.42 (standard deviation = 2.80); 9.35% of the participants scored ≤ 10 points, while 22.49% got more than 15 points. Multivariate linear regression analysis revealed that higher childbirth self-efficacy was significantly associated with younger age (ß = -0.05, P<0.01), lower body mass index (BMI) (ß = -0.06, P <0.01), receiving prenatal education (ß = 0.47, P <0.01), performing physical activity 6-7 times per week (ß = 0.47, P = 0.03), and fair (ß = 0.57, P <0.01) or good (ß = 1.08, P <0.01) self-rated health status.

Key Conclusions And Implications For Practice: Childbirth self-efficacy was low among pregnant Chinese women. Women with older age, higher BMI, and worse self-rated health status had lower childbirth self-efficacy. On the other hand, women who frequently exercised, had some prenatal education, and had better self-rated health showed higher childbirth self-efficacy during pregnancy. Interventions concentrating on improving childbirth self-efficacy in China should focus on prenatal education for expectant mothers, and more attention should be given to pregnant women who are older, obese, are less physically active, and are in poor health.
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http://dx.doi.org/10.1016/j.midw.2020.102883DOI Listing
February 2021

Visible-Light Photoredox-Catalyzed Formal [5 + 1] Cycloaddition of -Tosyl Vinylaziridines with Difluoroalkyl Halides.

Org Lett 2020 Dec 25;22(24):9658-9664. Epub 2020 Nov 25.

Institute of Functional Organic Molecular Engineering, College of Chemistry and Chemical Engineering, Henan University, Kaifeng 475004, People's Republic of China.

A visible-light photoredox-catalyzed formal [5 + 1] cycloaddition of -tosyl vinylaziridines with difluoroalkyl halides as unique C1 synthons was developed. The procedure provides an efficient and practical method to synthesize diverse pyridines in moderate to good yields. The reaction underwent a radical-initiated kinetically controlled ring-opening of vinylaziridines and involved a key α,β-unsaturated imine intermediate, followed by an E2 elimination, a 6π electrocyclization, and defluorinated aromatization.
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http://dx.doi.org/10.1021/acs.orglett.0c03718DOI Listing
December 2020

An inversion model for estimating the negative air ion concentration using MODIS images of the Daxing'anling region.

PLoS One 2020 24;15(11):e0242554. Epub 2020 Nov 24.

Heilongjiang Province Key Laboratory of Geographical Environment Monitoring and Spatial Information Service in Cold Regions, Harbin Normal University, Harbin, China.

The negative air ion (NAI) concentration is an essential indicator of air quality and atmospheric pollution. The NAI concentration can be used to monitor air quality on a regional scale and is commonly determined using field measurements. However, obtaining these measurements is time-consuming. In this paper, the relationship between remotely sensed surface parameters (such as land surface temperature, normalized difference vegetation index (NDVI), and leaf area index) obtained from MODIS data products and the measured NAI concentration using a stepwise regression method was analyzed to estimate the spatial distribution of the NAI concentration and verify the precision. The results indicated that the NAI concentration had a negative correlation with temperature, leaf area index (LAI), and gross primary production while it exhibited a positive correlation with the NDVI. The relationship between land surface temperature and the NAI concentration in the Daxing'anling region is expressed by the regression equation of y = -35.51x1 + 11206.813 (R2 = 0.6123). Additionally, the NAI concentration in northwest regions with high forest coverage was higher than that in southeast regions with low forest coverage, suggesting that forests influence the air quality and reduce the impact of environmental pollution. The proposed inversion model is suitable for evaluating the air quality in Daxing'anling and provides a reference for air quality evaluation in other areas. In the future, we will expand the quantity and distribution range of sampling points, conduct continuous observations of NAI concentrations and environmental parameters in the research areas with different land-use types, and further improve the accuracy of inversion results to analyze the spatiotemporal dynamic changes in NAI concentration and explore the possibility of expanding the application areas of NAI monitoring.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0242554PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7685430PMC
January 2021

The role of multimodal ultrasonic flow imaging in Thyroid Imaging Reporting and Data System (TI-RADS) 4 nodules.

Gland Surg 2020 Oct;9(5):1469-1477

Department of Ultrasound, Dongfang Hospital, Shanghai, China.

Background: Color Doppler imaging (CDFI), contrast-enhanced ultrasound (CEUS), and superb micro-vascular imaging (SMI) are used to observe blood flow characteristics in Thyroid Imaging Reporting and Data System (TI-RADS) 4 nodules. The ability of these techniques to distinguish benign from malignant nodules was investigated.

Methods: A total of 75 TI-RADS 4 nodules were examined using CDFI, SMI, and CEUS. The blood flow characteristics shown by the three methods were added to the current TI-RADS classification to establish a new TI-RADS classification. The value of the three methods and the diagnostic accuracy of the new and old TI-RADS classification were compared.

Results: SMI better captured type II flow in benign nodules and type III flow in malignant nodules relative to CDFI. Malignant nodules detected with CEUS manifested mainly with hypo-enhancement, whereas benign nodules showed iso- and hyper-enhancement. The areas under the receiver operating characteristic (ROC) curves (AUC) obtained through the aforementioned flow distribution models were 0.690 (CDFI), 0.840 (SMI), 0.910 (CEUS), and 0.903 (CEUS and SMI combined mode), respectively. The diagnostic value of CEUS was the highest. Joint inspection using SMI with CEUS showed certain advantages in sensitivity, although the overall accuracy was equal to that of CEUS alone. Except for CDFI, the AUC of the new TI-RADS classification was significantly higher than that of the old one. Perforating vessels and low enhancement were independent predictors of thyroid carcinoma.

Conclusions: Both SMI and CEUS visualized lower-velocity blood flow within TI-RADS 4 nodules. The new TI-RADS classification described here could improve diagnostic accuracy.
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http://dx.doi.org/10.21037/gs-20-641DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7667111PMC
October 2020

Development of an efficient pathway construction strategy for rapid evolution of the biodegradation capacity of Pseudomonas putida KT2440 and its application in bioremediation.

Sci Total Environ 2021 Mar 26;761:143239. Epub 2020 Oct 26.

Key Laboratory of Molecular Microbiology and Technology for Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China. Electronic address:

In this work, we developed an efficient pathway construction strategy, consisting of DNA assembler-assisted pathway assembly and counterselection system-based chromosomal integration, for the rapid and efficient integration of synthetic biodegradation pathways into the chromosome of Pseudomonas putida KT2440. Using this strategy, we created a novel degrader capable of complete mineralization of γ-hexachlorocyclohexane (γ-HCH) and 1,2,3-trichloropropane (TCP) by integrating γ-HCH and TCP biodegradation pathways into the chromosome of P. putida KT2440. Furthermore, the chromosomal integration efficiencies of γ-HCH and TCP biodegradation pathways were improved to 50% and 41.6% in P. putida KT2440, respectively, by the inactivation of a type I DNA restriction-modification system. The currently developed pathway construction strategy coupled with the mutant KTUΔhsdRMS will facilitate implantation of heterologous catabolic pathways into the chromosome for rapid evolution of the biodegradation capacity of P. putida. More importantly, the successful removal of γ-HCH (10 mg/kg soil) and TCP (0.2 mM) from soil and wastewater within 14 days, respectively, highlighted the potential of the novel degrader for in situ bioremediation of γ-HCH- and TCP-contaminated sites. Moreover, chromosomal integration of gfp made the degrader to be monitored easily during bioremediation. In the future, this strategy can be expanded to a broad range of bacterial species for widespread applications in bioremediation.
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http://dx.doi.org/10.1016/j.scitotenv.2020.143239DOI Listing
March 2021

Psychological Behavior of Frontline Medical Staff in the Use of Preventive Medication for COVID-19: A Cross-Sectional Study.

Front Psychol 2020 25;11:2104. Epub 2020 Sep 25.

Department of Health Toxicology, Xiangya School of Public Health, Central South University, Changsha, China.

Purpose: To understand the current pandemic, levels of anxiety in frontline staff, and whether they have been using medication to prevent COVID-19.

Methods: Between January 10 and March 10, 2020, 290 frontline staff completed a questionnaire incorporating the Generalized Anxiety Disorder Scale 7 (GAD-7) to indicate their psychological behavior in the use of preventive medication.

Results: Of those who participated in the study, 77.6% used preventive medication, with 47.5, 40.9, and 11.6% using these as part of routine preventive treatment, to fight infection after it was contracted, and after occupational exposure, respectively. There was a statistically significant relationship between the anxiety scale scores and the frequency of medication use ( < 0.05). Comparative analyses revealed that the scores of those in the group taking medication after occupational exposure (to respiratory and blood-borne pathogens) were significantly different from other groups. The proportion of participants choosing Western medicine, traditional Chinese medicine, and integrated Chinese and Western medicine was 24.4, 28.0, and 47.6%, respectively. Additionally, the relationship between the anxiety scale scores and the three types of medication was statistically significant ( < 0.05), as was the difference between Western medicine and other groups. According to Multinomial logistic regression based on the adjustment to gender, age, educational level, marital status, current workplace, and profession, participants with moderate to severe anxiety, had higher odds ( = 10.331, 95%CI:1.453-73.429) of using Western medicine than participants with no anxiety. Participants with moderate anxiety were 6.399 times more likely to use an integrated combination of traditional Chinese and Western medicine compared to those with no anxiety ( = 6.399, 95%CI:1.007-40.658). Furthermore, those with mild anxiety were 2.656 times more likely to use integrated traditional Chinese and Western medicine than those with no anxiety ( = 2.657, 95%CI:1.075-6.570). The probability that frontline medical staff with moderate anxiety took preventive medication after occupational exposure to COVID-19 was 8.066 times ( = 8.066, 95%CI:1.043-62.353) higher than that of staff without anxiety.

Discussion: This study revealed that there was more anxiety among frontline medical staff who took medication after unexpected occupational exposure. There was less anxiety among those using an integrated course of Chinese and Western medicine than Western medicine alone. It was also observed that anxiety affects the types and frequency of the preventive medication taken. Frontline medical staff who suffer from anxiety are also more likely to use medication to prevent COVID-19.
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http://dx.doi.org/10.3389/fpsyg.2020.02104DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7545108PMC
September 2020

Development and nationwide application of an antibiotic knowledge scale.

Prev Med 2020 12 3;141:106262. Epub 2020 Oct 3.

School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, PR China. Electronic address:

Misconceptions about antibiotics among the public can potentially lead to their inappropriate use. Currently, there is no antibiotic knowledge assessment tool to address this issue. This study aimed to develop and validate an antibiotic knowledge scale (AKS) and apply this scale to assess public knowledge about antibiotics in China. An initial 18-item AKS was designed and validated among 1180 people recruited in June 2017. After removing redundant items, the reliability and validity of the AKS were examined. Subsequently, a nationwide survey was conducted, and 12,772 people were recruited using multistage sampling and surveyed using the developed AKS. A logistic regression model was used to identify the factors associated with poor knowledge about antibiotics. The final AKS included two screening items and fifteen knowledge evaluation items. Cronbach's alpha, test-retest reliability, and split-half reliability were 0.91, 0.88, and 0.89, respectively. These knowledge evaluation items were loaded in four distinct factors that explained 70.72% of cumulative variance among respondents. Using the developed AKS to assess public knowledge about antibiotics among 12,772 participants, the mean score on the AKS was 7.25 and 67% of participants had poor antibiotic knowledge, which was associated with male gender, rural residence, lower educational level, poor economic status, living in western China, and lacking education on antibiotics. The AKS demonstrated satisfactory reliability and validity in identifying the population with poor antibiotic knowledge. Importantly, the majority of participants had inadequate knowledge about antibiotics. Thus, it is necessary to conduct interventions focusing on improving public knowledge about antibiotics.
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http://dx.doi.org/10.1016/j.ypmed.2020.106262DOI Listing
December 2020

An IoT-enabled paper sensor platform for real-time analysis of isothermal nucleic acid amplification tests.

Biosens Bioelectron 2020 Dec 24;169:112651. Epub 2020 Sep 24.

Department of Electrical and Computer Engineering, Iowa State University, Ames, IA, 50011, USA; Department of Mechanical Engineering, Iowa State University, Ames, IA, 50011, USA. Electronic address:

Paper-based sensors can be exploited to develop low-cost, disposable, and rapid assays for the detection of a large variety of analytes. We report a paper-based sensor system for a point-of-care (POC) nucleic acid amplification test that can quantitatively detect multiple genes from different pathogens. The POC system combines a paper sensor chip and a portable instrument, which is built on an Internet of Things (IoT) platform. The paper-based sensor provides the functions of reagent storage, sample transportation, and nucleic acid amplification. The IoT instrument uses an Arduino microcontroller to control temperature, collect fluorescence images, and store the data in cloud storage via a WiFi network. A compact fluorescence reader was designed to measure fluorescence images of the amplicons during a loop-mediated isothermal amplification reaction in real-time. The real-time detection capability enables the quantitative analysis of target genes. The results show that the paper-based sensor cam distinguish multiple genes of the genomic DNA extracted from Escherichia coli and Campylobacter jejuni, with the concentration as low as 2 × 10 copies/μL. The affordable instrument, in conjunction with the disposable paper sensor chip, would have a great potential for POC detections of pathogens.
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http://dx.doi.org/10.1016/j.bios.2020.112651DOI Listing
December 2020

Genome reduction enhances production of polyhydroxyalkanoate and alginate oligosaccharide in Pseudomonas mendocina.

Int J Biol Macromol 2020 Nov 15;163:2023-2031. Epub 2020 Sep 15.

Key Laboratory of Molecular Microbiology and Technology for Ministry of Education, Nankai University, Tianjin 300071, China. Electronic address:

Pseudomonas mendocina NK-01 previously isolated by our lab is able to accumulate medium-chain-length polyhydroxyalkanoate (mcl-PHA) intracellularly and secrete alginate oligosaccharide (AO) to the extracellular milieu. The present study aimed at investigating whether improved production of mcl-PHA and AO by P. mendocina can be accomplished by genome reduction. In this study, 14 large genomic fragments accounting for 7.7% of the genome of P. mendocina NK-01 were sequentially deleted to generate a series of genome-reduced strains by an upp-based markerless knockout method. As a result, the intracellular ATP/ADP ratio of the strain NKU421 with the largest deletion improved by 11 times compared to NK-01. More importantly, the mcl-PHA and AO yields of NKU421 increased by 114.8% and 27.8%, respectively. Enhancing mcl-PHA and AO production by NKU421 may be attributed to improved transcriptional levels of PHA synthase genes and AO secretion-related genes. The present study suggests that rational reduction of bacterial genome is a feasible approach to construct an optimal chassis for enhanced production of bacterial metabolites. In the future, further reduction of the NKU421 genome can be expected to create high-performance chassis for the development of microbial cell factories.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.09.067DOI Listing
November 2020

The influence of socioeconomic status on menarcheal age among Chinese school-age girls in Tianjin, China.

Eur J Pediatr 2021 Mar 12;180(3):825-832. Epub 2020 Sep 12.

Department of Epidemiology and Biostatistics, School of Public Health, Tianjin Medical University, Tianjin, China.

The association between socioeconomic status and the onset age of menarche is still not conclusive. This cross-sectional study was conducted among primary and middle school girls aged 7 to 16 years old in Tianjin, China, to explore the distribution of menarcheal age and its association with socioeconomic status. A self-designed structured questionnaire was completed by students and their parents to collect information on socioeconomic status (i.e., family income, parental education level, living residence), menarcheal status, and covariates (weight status of girls and their parents, sleeping hours per day, physical activity). Information on menarcheal status included whether or not menarche had occurred (Yes/No) and the exact age at menarche. Linear regression analysis was used to explore the association between socioeconomic status and menarcheal age before and after covariate adjustment. Among 1485 eligible girls with complete information, 445 had experienced menarche, with an overall menarche rate of 30%. The mean age at menarche was 12.9 years (95% confidence interval 12.8-13.0). Urban girls experienced menarche earlier than rural girls did (12.1 years vs. 13.5 years). Univariate analysis showed that urban residence and higher parental education were associated with earlier onset of menarche. After covariate adjustment, the significance still existed. However, after adjusting further for residence, the significant association with the parental education disappeared. Only urban residence was still significantly associated, even after further adjustment for parental education and family income, with adjusted regression coefficients (95% confidence interval) of - 1.087 (- 1.340,-0.834), indicating that the onset age of menarche among urban girls was 1.087 years (0.834, 1.340) younger than that among rural girls. Family income was not related to the onset age of menarche in any analyses.Conclusion: Urban-rural differences played a more important role in the early onset of menarche than socioeconomic differences between families. What is Known: • The age at menarche varies by race and country, but the global trend is towards earlier onset as a result of changes in nutrition, family structure, socioeconomic status, and physical condition. What is New: • Urban girls experienced menarche earlier than rural girls, but this urban-rural difference could not be explained by family income, parental education, weight status of the participants and their parents, participants' physical exercise and sleeping hours. • Higher parental education was associated with earlier onset of menarche, but this association disappeared after adjustment for living residence.
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http://dx.doi.org/10.1007/s00431-020-03803-4DOI Listing
March 2021

Factors Associated With Job Satisfaction of Frontline Medical Staff Fighting Against COVID-19: A Cross-Sectional Study in China.

Front Public Health 2020 4;8:426. Epub 2020 Aug 4.

Department of Clinical Nursing, The Second Xiangya Hospital of Central South University, Changsha, China.

The current research on frontline medical staff in China fighting against COVID-19 has not yet addressed job satisfaction. The purpose of this study is to investigate the job satisfaction of those who were sent to support Hubei province, China, or worked in local designated hospitals, and then analyze the associated influencing factors. A total of 455 medical staff who worked at the frontline of the prevention and control of COVID-19 in Hubei province was selected using simple random sampling. They were asked to fill out a self-developed general information questionnaire as well as the Minnesota Satisfaction Questionnaire (MSQ), from 10 January to 10 March 2020. The average job satisfaction score of the participants was 82.58 ± 11.11. The influencing factors include education ( = 0.002), years of work experience = 0.006), anti-epidemic work duration ( = 0.048), daily sleep duration ( < 0.001), and the form of participation ( < 0.001). This study, for the first time, measures the job satisfaction of frontline medical staff in fighting against COVID-19 in China. The job satisfaction of frontline medical staff was at a "relatively decent" level, higher than the previous similar measures among medical staff. Related management departments should further improve the job satisfaction of frontline medical staff by meeting their reasonable demands, strengthening the emergency response and practical operation training of junior staff, and ensuring their ample time for sleep and rest. This study is of great reference value for improving the job satisfaction level of frontline medical staff during public health emergencies, developing medical staff security policies, and promoting the establishment of emergency response teams.
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http://dx.doi.org/10.3389/fpubh.2020.00426DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7417651PMC
August 2020

Impact of updated pediatric hypertension criteria on prevalence estimates of hypertension among Chinese children.

J Hum Hypertens 2020 Jun 22. Epub 2020 Jun 22.

Department of Epidemiology and Biostatistics, School of Public Health, and Tianjin Key Laboratory of Environment, Nutrition & Public Health, Tianjin Medical University, Tianjin, China.

This study aimed to evaluate the impact of updated pediatric hypertension (HTN) criteria (the 2017 AAP Guidelines) on prevalence estimates of HTN and prehypertension among Chinese children compared to the 2004 Fourth Report. A total of 2093 children aged 7-15 years from five schools in Tianjin, China were selected using a multistage random cluster sampling method. The prevalence of HTN per the 2017 AAP Guidelines (10.1%) was significantly higher than that per the Fourth Report (6.6%), whereas the prevalence of prehypertension per the 2017 AAP Guidelines (6.3%) was significantly lower than that per the Fourth Report (8.8%). From the 2004 Fourth Report to the 2017 AAP Guidelines, a total of 117 (5.6%) children were reclassified to have higher blood pressure. The two criteria had better consistency in the diagnosis of systolic abnormalities than in the diagnosis of diastolic abnormalities. The updated definitions for pediatric HTN have a substantive impact on the prevalence estimation among Chinese children, especially among boys, overweight children, and older children.
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http://dx.doi.org/10.1038/s41371-020-0370-0DOI Listing
June 2020

Leveraging the Hermes Transposon to Accelerate the Development of Nonconventional Yeast-based Microbial Cell Factories.

ACS Synth Biol 2020 07 16;9(7):1736-1752. Epub 2020 Jun 16.

Department of Chemical and Biological Engineering, Iowa State University, Ames, Iowa, United States.

We broadened the usage of DNA transposon technology by demonstrating its capacity for the rapid creation of expression libraries for long biochemical pathways, which is beyond the classical application of building genome-scale knockout libraries in yeasts. This strategy efficiently leverages the readily available fine-tuning impact provided by the diverse transcriptional environment surrounding each random integration locus. We benchmark the transposon-mediated integration against the nonhomologous end joining-mediated strategy. The latter strategy was demonstrated for achieving pathway random integration in other yeasts but is associated with a high false-positive rate in the absence of a high-throughput screening method. Our key innovation of a nonreplicable circular DNA platform increased the possibility of identifying top-producing variants to 97%. Compared to the classical DNA transposition protocol, the design of a nonreplicable circular DNA skipped the step of counter-selection for plasmid removal and thus not only reduced the time required for the step of library creation from 10 to 5 d but also efficiently removed the "transposition escapers", which undesirably represented almost 80% of the entire population as false positives. Using one endogenous product (, shikimate) and one heterologous product (, ()-norcoclaurine) as examples, we presented a streamlined procedure to rapidly identify high-producing variants with titers significantly higher than the reported data in the literature. We selected , a representative nonconventional yeast, as a demo, but the strategy can be generalized to other nonconventional yeasts. This new exploration of transposon technology, therefore, adds a highly versatile tool to accelerate the development of novel species as microbial cell factories for producing value-added chemicals.
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http://dx.doi.org/10.1021/acssynbio.0c00123DOI Listing
July 2020

An AUV localization and path planning algorithm for terrain-aided navigation.

ISA Trans 2020 Aug 23;103:215-227. Epub 2020 Apr 23.

Science and Technology on Underwater Vehicle Laboratory, Harbin Engineering University, Harbin 150001, China.

Terrain-aided navigation (TAN) holds high potential for long-term accurate navigation of autonomous underwater vehicles (AUVs), and path planning algorithms are essential in TAN to decrease positioning errors by avoiding flat areas. This study proposed an AUV localization and path planning algorithm for TAN, which consists of a value function calculation and online path planning. In the value function calculation, the topographic complexity is treated as a factor that influences AUV state transition probabilities to calculate the optimal policy; meanwhile, the online path planning applies a particle filter to localize and command AUVs, and particle weights are calculated according to topographic complexity. Simulation experimental results demonstrate that this algorithm could provide paths with accurate TAN location results and good maneuvering performance.
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http://dx.doi.org/10.1016/j.isatra.2020.04.007DOI Listing
August 2020

Chinese Public's Attention to the COVID-19 Epidemic on Social Media: Observational Descriptive Study.

J Med Internet Res 2020 05 4;22(5):e18825. Epub 2020 May 4.

Department of Social Medicine and Health Management, Xiangya School of Public Health, Central South University, Changsha, China.

Background: Since the coronavirus disease (COVID-19) epidemic in China in December 2019, information and discussions about COVID-19 have spread rapidly on the internet and have quickly become the focus of worldwide attention, especially on social media.

Objective: This study aims to investigate and analyze the public's attention to events related to COVID-19 in China at the beginning of the COVID-19 epidemic (December 31, 2019, to February 20, 2020) through the Sina Microblog hot search list.

Methods: We collected topics related to the COVID-19 epidemic on the Sina Microblog hot search list from December 31, 2019, to February 20, 2020, and described the trend of public attention on COVID-19 epidemic-related topics. ROST Content Mining System version 6.0 was used to analyze the collected text for word segmentation, word frequency, and sentiment analysis. We further described the hot topic keywords and sentiment trends of public attention. We used VOSviewer to implement a visual cluster analysis of hot keywords and build a social network of public opinion content.

Results: The study has four main findings. First, we analyzed the changing trend of the public's attention to the COVID-19 epidemic, which can be divided into three stages. Second, the hot topic keywords of public attention at each stage were slightly different. Third, the emotional tendency of the public toward the COVID-19 epidemic-related hot topics changed from negative to neutral, with negative emotions weakening and positive emotions increasing as a whole. Fourth, we divided the COVID-19 topics with the most public concern into five categories: the situation of the new cases of COVID-19 and its impact, frontline reporting of the epidemic and the measures of prevention and control, expert interpretation and discussion on the source of infection, medical services on the frontline of the epidemic, and focus on the worldwide epidemic and the search for suspected cases.

Conclusions: Our study found that social media (eg, Sina Microblog) can be used to measure public attention toward public health emergencies. During the epidemic of the novel coronavirus, a large amount of information about the COVID-19 epidemic was disseminated on Sina Microblog and received widespread public attention. We have learned about the hotspots of public concern regarding the COVID-19 epidemic. These findings can help the government and health departments better communicate with the public on health and translate public health needs into practice to create targeted measures to prevent and control the spread of COVID-19.
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http://dx.doi.org/10.2196/18825DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7199804PMC
May 2020

Superaerophilic copper nanowires for efficient and switchable CO electroreduction.

Nanoscale Horiz 2019 Mar 28;4(2):490-494. Epub 2018 Nov 28.

State Key Laboratory of Chemical Resource Engineering, Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, Beijing 100029, P. R. China.

Copper is one of the most efficient electrocatalysts for switchable carbon dioxide conversion, but the design of an advanced Cu-based catalyst with high selectivity while suppressing hydrogen evolution remains a great challenge. Herein, we use Cu nanowires (Cu NWs) as the starting materials and polytetrafluoroethylene (PTFE) as the surface modifier to make a superaerophilic electrode using a wettability control strategy. This strategy allows tuning of the selectivity of the CO reduction reaction (CORR) and a decrease of the hydrogen evolution rate simultaneously by facilitating the supply of CO reactants and inhibiting the adsorption of water (protons). The transferring point from a pinning to bursting state turned out to be the optimized condition leading to the highest CORR faradaic efficiency without significant interference of current density. The optimized superaerophilic Cu NW catalyst showed CO-selectivity with a Faraday efficiency of 71% at -0.4 V vs. RHE and HCOOH-selectivity with a Faraday efficiency of 68% at -0.6 V vs. RHE. Moreover, the accelerated gas and ion diffusion and homogenized reactions also avoided accumulative damage on the surface of the Cu NWs and enhanced the stability of the Cu catalyst. This wettability tuning strategy provides a facile and efficient way to optimize the gas and ion diffusion layers, therefore promoting the performance of the CORR. This strategy potentially can be extended to the design of other gas consumption electrocatalysts.
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http://dx.doi.org/10.1039/c8nh00259bDOI Listing
March 2019

Modulating Pathway Performance by Perturbing Local Genetic Context.

ACS Synth Biol 2020 04 24;9(4):706-717. Epub 2020 Mar 24.

Interdepartmental Microbiology Program, Iowa State University, Ames, Iowa 50011, United States.

Combinatorial engineering is a preferred strategy for attaining optimal pathway performance. Previous endeavors have been concentrated on regulatory elements (, promoters, terminators, and ribosomal binding sites) and/or open reading frames. Accumulating evidence indicates that noncoding DNA sequences flanking a transcriptional unit on the genome strongly impact gene expression. Here, we sought to mimic the effect imposed on expression cassettes by the genome. We created variants of the model yeast with significantly improved fluorescence or cellobiose consumption rate by randomizing the sequences adjacent to the GFP expression cassette or the cellobiose-utilization pathway, respectively. Interestingly, nucleotide specificity was observed at certain positions and showed to be essential for achieving optimal cellobiose assimilation. Further characterization suggested that the modulation effects of the short sequences flanking the expression cassettes could be potentially mediated by remodeling DNA packaging and/or recruiting transcription factors. Collectively, these results indicate that the often-overlooked contiguous DNA sequences can be exploited to rapidly achieve balanced pathway expression, and the corresponding approach could be easily stacked with other combinatorial engineering strategies.
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http://dx.doi.org/10.1021/acssynbio.9b00445DOI Listing
April 2020

Deletion of genomic islands in the Pseudomonas putida KT2440 genome can create an optimal chassis for synthetic biology applications.

Microb Cell Fact 2020 Mar 18;19(1):70. Epub 2020 Mar 18.

State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, 300071, China.

Background: Genome streamlining is a feasible strategy for constructing an optimum microbial chassis for synthetic biology applications. Genomic islands (GIs) are usually regarded as foreign DNA sequences, which can be obtained by horizontal gene transfer among microorganisms. A model strain Pseudomonas putida KT2440 has broad applications in biocatalysis, biotransformation and biodegradation.

Results: In this study, the identified GIs in P. putida KT2440 accounting for 4.12% of the total genome size were deleted to generate a series of genome-reduced strains. The mutant KTU-U13 with the largest deletion was advantageous over the original strain KTU in several physiological characteristics evaluated. The mutant KTU-U13 showed high plasmid transformation efficiency and heterologous protein expression capacity compared with the original strain KTU. The metabolic phenotype analysis showed that the types of carbon sources utilized by the mutant KTU-U13 and the utilization capabilities for certain carbon sources were increased greatly. The polyhydroxyalkanoate (PHA) yield and cell dry weight of the mutant KTU-U13 were improved significantly compared with the original strain KTU. The chromosomal integration efficiencies for the γ-hexachlorocyclohexane (γ-HCH) and 1,2,3-trichloropropane (TCP) biodegradation pathways were improved greatly when using the mutant KTU-U13 as the recipient cell and enhanced degradation of γ-HCH and TCP by the mutant KTU-U13 was also observed. The mutant KTU-U13 was able to stably express a plasmid-borne zeaxanthin biosynthetic pathway, suggesting the excellent genetic stability of the mutant.

Conclusions: These desirable traits make the GIs-deleted mutant KTU-U13 an optimum chassis for synthetic biology applications. The present study suggests that the systematic deletion of GIs in bacteria may be a useful approach for generating an optimal chassis for the construction of microbial cell factories.
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http://dx.doi.org/10.1186/s12934-020-01329-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7081699PMC
March 2020

Two-Dimensional Amorphous SnO from Liquid Metal: Mass Production, Phase Transfer, and Electrocatalytic CO Reduction toward Formic Acid.

Nano Lett 2020 Apr 13;20(4):2916-2922. Epub 2020 Mar 13.

Department of Chemistry, School of Science, Tianjin Key Laboratory of Molecular Optoelectronic Science, Tianjin University, Tianjin 300072, China.

Liquid metal forms a thin layer of oxide skin via exposure to oxygen and this layer could be exfoliated by mechanical delamination or gas-injection/solvent-dispersion. Although the room-temperature fabrication of two-dimensional (2D) oxide through gas-injection and water-dispersion has been successfully demonstrated, a synthetic protocol in nonaqueous solvent at elevated temperature still remains as a challenge. Herein we report the mass-production of amorphous 2D SnO nanoflakes with Bi decoration from liquid Sn-Bi alloy and selected nonaqueous solvents. The functional groups of the solvents play a key role in determining the final morphology of the product and the hydroxyl-rich solvents exhibit the best control toward 2D SnO. The different solvent-oxide interaction that facilitates this phase-transfer process is further discussed on the basis of DFT calculation. Finally, the as-obtained 2D SnO is evaluated in electrocatalytic CO reduction with high faradaic efficiency (>90%) of formic acid and stable performance over 10 h.
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http://dx.doi.org/10.1021/acs.nanolett.0c00844DOI Listing
April 2020

Candidate lncRNA-microRNA-mRNA networks in predicting non-small cell lung cancer and related prognosis analysis.

J Cancer Res Clin Oncol 2020 Apr 2;146(4):883-896. Epub 2020 Mar 2.

China Medical University, Shenyang, 110122, China.

Purpose: The role of non-coding RNA, once thought to be dark matter, is increasingly prominent in cancer. Our article explores the effect of non-coding RNA in lung adenocarcinoma and lung squamous cell carcinoma by mining TCGA public database.

Methods: Download the data by applying the official TCGA software. The data were analyzed by R data analysis packages, 'edgeR', 'gplots' and 'survival'. We better illustrate the potential networks of lung cancer genes by constructing ceRNAs, using Cytoscape software.

Results: We obtained genes which were differentially expressed in lung adenocarcinoma and lung squamous cell carcinoma analysis. Within these differentially expressed genes, we also conducted a survival analysis to find differentially expressed genes associated with prognosis in both lung adenocarcinoma and lung squamous cell carcinoma. Based on genes differentially expressed of both lung adenocarcinoma and lung squamous cell carcinoma, we constructed a ceRNA network to illustrate the mechanism of lung adenocarcinoma and lung squamous cell carcinoma. Our study analyzed genes which were differentially expressed in lung adenocarcinoma and lung squamous cell carcinoma using the TCGA database.

Conclusion: Based on this, the prognosis in both lung squamous cell carcinoma and lung adenocarcinoma was analyzed. We have also constructed a ceRNA network to provide a basis for the study of ceRNA in lung adenocarcinoma and lung squamous cell carcinoma.
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http://dx.doi.org/10.1007/s00432-020-03161-6DOI Listing
April 2020

A case of liver cancer identified by Leser-Trélat's sign.

Dig Liver Dis 2020 03 7;52(3):353. Epub 2020 Jan 7.

Department of Dermatology, The Second Affiliated Hospital of Guilin Medical University, Guilin, Guangxi, China. Electronic address:

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http://dx.doi.org/10.1016/j.dld.2019.12.001DOI Listing
March 2020

Post-stroke medication adherence and persistence rates: a meta-analysis of observational studies.

J Neurol 2019 Dec 2. Epub 2019 Dec 2.

School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, People's Republic of China.

Background: Following a doctor's prescribed medication regimen is the key to prevent recurrent stroke and adverse outcomes. Many studies have investigated post-stroke drug adherence and persistence in patients. However, a comprehensive analysis of the data is lacking.

Objectives: A meta-analysis of published literature was conducted to summarize the ratio of medication adherence and persistence in patients after stroke.

Methods: Relevant studies were identified by conducting a literature search using PubMed, EMBASE and Web of Science up to April 2019. We also reviewed the reference list of the retrieved articles to identify additional studies. We included observational studies that reported data on patients' medication adherence or persistence status, or the rate of medication adherence or persistence among patients with stroke could be calculated based on the information provided.

Results: The overall high medication adherence rate of patients with stroke was 64.1% (95% CI: 57.4%-70.8%), and the persistence rate of patients with stroke was 72.2% (95% CI: 69.1%-75.3%). The highest persistence rate was observed in cohort studies which was 80.1% (95% CI: 76.7%-83.4%). The medication adherence rate was the highest in cases where the rates were assessed through interviews or self-reports (77.7% (95% CI: 71.3%-84.1%)).

Conclusions: Medication adherence and persistence rates are low in patients after suffering a stroke. Patient medication adherence or persistence and their influencing factors should be considered for the treatment of stroke patients. More detailed disease prevention and management strategies need to be developed for stroke patients with different comorbidities.
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http://dx.doi.org/10.1007/s00415-019-09660-yDOI Listing
December 2019

Association between prenatal exposure to bisphenol a and birth outcomes: A systematic review with meta-analysis.

Medicine (Baltimore) 2019 Nov;98(44):e17672

Department of Epidemiology, School of Public Health, Dalian Medical University.

Background: Previous studies investigated the relation of prenatal exposure to bisphenol A (BPA) and birth outcomes, but these results were inconsistent. The aim of this study was to investigate the relation of prenatal exposure to BPA and birth outcomes, provide comprehensive results based on current studies.

Methods: The PubMed, Cochrane databases, and Web of Science databases were searched systematically by two researchers respectively from their inceptions to Oct. 2018, using the following keywords "bisphenol A, birth weight, birth length, head circumference, gestational age, birth outcomes". We extracted β coefficient and 95% confidence interval (CI) or β coefficient and standard deviation (SD) from included study. The subgroup analysis was performed to evaluate the potential heterogeneity between studies. We conducted sensitivity analysis by excluding the each individual study to assess the results whether were stable. Finally, the publication bias was performed by accumulative forest plot.

Results: Seven studies with 3004 participants met the inclusion criteria. BPA had significant positively association with birth weight (β = 21.92, 95%CI: 1.50-42.35, P = .04). No significant associations were found between BPA and birth length, head circumference and gestational age (All of P > .05).

Conclusion: This meta-analysis demonstrated that the BPA was positively associated with birth weight. Therefore, further studies are needed to investigate the critical sensitive period of influencing fetal development and to investigate the difference on gender.
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http://dx.doi.org/10.1097/MD.0000000000017672DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6946218PMC
November 2019

Metal Nitrate Catalysis for Selective Oxidation of 5-Hydroxymethylfurfural into 2,5-Diformylfuran under Oxygen Atmosphere.

ACS Omega 2019 Apr 18;4(4):7054-7060. Epub 2019 Apr 18.

Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources and College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, People's Republic of China.

Selective synthesis of various versatile compounds from biomass is of great importance to displace traditional fossil fuel resources. Here, homogeneous metal nitrate (M(NO) )/(2,2,6,6-tetramethylpiperidin-1-yl)oxyl (TEMPO) and M(NO) /TEMPO/NaNO catalyst systems in glacial acetic acid and acetonitrile, respectively, have been found to be highly active and practically sustainable for selective oxidation of 5-hydroxymethylfurfural (HMF) into 2,5-diformylfuran (DFF) using pure O or even O in air as the oxidant. The catalytic methods enable full HMF conversion with a nearly 100% DFF selectivity at 50 °C under atmospheric pressure using a very simple reaction setup and workup. Mechanistic aspects are discussed. The influences of reaction conditions such as different metal catalysts, catalyst loading, solvents, and reaction temperature on the promotion effect were studied. Meanwhile, the catalyst systems had also good performance for aerobic oxidation of other alcohols.
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http://dx.doi.org/10.1021/acsomega.9b00391DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6648045PMC
April 2019