Publications by authors named "Yuxia Liu"

109 Publications

Facile In Situ Chemical Cross-Linking Gel Polymer Electrolyte, which Confines the Shuttle Effect with High Ionic Conductivity and Li-Ion Transference Number for Quasi-Solid-State Lithium-Sulfur Battery.

ACS Appl Mater Interfaces 2021 Sep 10. Epub 2021 Sep 10.

School of Chemical Engineering, Sichuan University, No. 24 South Section 1, Yihuan Road, Chengdu 610065, P. R. China.

As a secondary Li-ion battery with high energy density, lithium-sulfur (Li-S) batteries possess high potential development prospects. One of the important ingredients to improve the safety and energy density in Li-S batteries is the solid-state electrolyte. However, the poor ionic conductivity largely limits its application for the commercial market. At present, the gel electrolyte prepared by combining the electrolyte or ionic liquid with the all-solid electrolyte is an effective method to solve the low ion conductivity of the solid electrolyte. We present a cross-linked gel polymer electrolyte with fluoroethylene carbonate (FEC) as a solid electrolyte interface (SEI) film formed for Li-S quasi-solid-state batteries, which can be simply synthesized without initiators. This gel polymer electrolyte with FEC as an additive ([email protected]) possesses high ionic conductivity (0.830 × 10 S/cm at 25 °C and 1.577 × 10 S/cm at 85 °C) and extremely high Li-ion transference number ( = 0.674). In addition, the strong ability toward anchoring polysulfides resulting in the high electrochemical performance of Li-S batteries was confirmed in [email protected] by the diffusion experiment, X-ray photoelectron spectroscopy analysis (XPS), and scanning electron microscopy (SEM) mapping of the S element. Such a high ion conductivity (IC) gel polymer electrolyte enables a competitive specific capacity of 940 mAh/g at 0.2C and supreme cycling performance for 180 cycles at 0.5C, which is far beyond that of conventional poly(ethylene oxide)-based quasi-solid-state Li-S batteries.
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http://dx.doi.org/10.1021/acsami.1c16148DOI Listing
September 2021

Novel functional separator with self-assembled MnO layer via a simple and fast method in lithium-sulfur battery.

J Colloid Interface Sci 2021 Aug 11;606(Pt 1):666-676. Epub 2021 Aug 11.

School of Mechanical Engineering, Chengdu University, Chengdu 610106, China.

Modifying separator with metal oxides has been considered as a strong strategy to inhibit the shuttling of soluble polysulfide in the lithium-sulfur battery (Li-S battery). Manganesedioxide (MnO), one kind of transition metal oxide, is widely applied to decorate the PP (Polypropylene) separator. However, the fabrication by physical coating is always multistep and complicated. Here, we design a simple and fast method to chemically decorate separator. Based on the oxidizing property of acidic KMnO solution, the PP separator was oxidized and an ultrathin self-assembled MnO layer was directly constructed on one side of separator, by immersing in acidic KMnO solution for only 1 h. The self-assembled MnO layer has the synergistic effect of adsorption and catalytic conversion on polysulfides, which can effectively inhibit the shuttle effect. It can also help battery to maintain excellent electrochemical kinetics in the electrochemical cycle and maintain the effective recycling of active substances. As a result, the shuttling of polysulfide is greatly prohibited by this novel functional separator, and cycling stability is outstandingly improved, with a low-capacity decaying of 0.058% after 500 cycles at 0.5C. The rapid and simple modification method proposed in this study has a certain reference value for the future large-scale application of lithium-sulfur battery.
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http://dx.doi.org/10.1016/j.jcis.2021.08.062DOI Listing
August 2021

Microstructure-Controlled Li-Rich Mn-Based Cathodes by a Gas-Solid Interface Reaction for Tackling the Continuous Activation of LiMnO.

ACS Appl Mater Interfaces 2021 Sep 20;13(34):40995-41003. Epub 2021 Aug 20.

College of Chemical Engineering, Sichuan University, Chengdu 610065, P.R. China.

Li-rich Mn-based cathodes have attracted much attention due to their high capacity stemming from anion redox above 4.5 V. However, the continuous activation of LiMnO in Li-rich Mn-based materials, which correlates with O release and TM migration, is usually unfavorable to structural stability. Herein, based on a gas-solid interface reaction, we tackle this continuous activation phenomenon by restricting the capacity release of LiMnO via NHHCO treatment in the LiNiMnO cathode. After modification, oxygen vacancies associated with the spinel phase are introduced on the surface. The 4 mol % NHHCO-modified material's capacity starts at 182 mAh g at 1 C instead of increasing from 173 to 186 mAh g for 25 cycles in the pristine material. Meanwhile, it also exhibits an excellent capacity retention of 93.24% after 200 cycles (at 1 C), with a small voltage decay rate of 1.19 mV cycle.
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http://dx.doi.org/10.1021/acsami.1c12221DOI Listing
September 2021

First Report of Dasheen Mosaic Virus Infecting Typhonium giganteum Engl. (Baifuzi) in Henan Province of China.

Plant Dis 2021 Aug 18. Epub 2021 Aug 18.

Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, China;

Typhonium giganteum Engl. (Baifuzi ) is a perennial plant of the family Araceae. In China, its root is commonly used as an antispasmodic for stroke and cancer treatment (Chi et al. 2010; Gao et al. 2014; Khalivulla et al. 2019). Yuzhou city in Henan Province is the main producing area of T. giganteum Engl., and in July 2020, a survey of viral disease infecting T. giganteum Engl. was conducted in the city. In the surveyed fields (n =5), over 60% of plants displayed varying levels of virus-like symptoms, including mosaic, chlorotic and leaf distortion (Supplementary Figure S1) . To identify possible viral pathogens associated with the disease symptoms afflicting T. giganteum Engl., one leaf each from 25 symptomatic plants was collected and analyzed by high-throughput sequencing (HTS) as well as PCR. For HTS analysis, total RNA was extracted from one pooled sample containing a portion of all abovementioned leaves using RNAprep Pure Plant Plus Kit (TIANGEN Biotech, Beijing, China). After removing ribosomal RNA with Ribo-off rRNA depletion kit (Vazyme Biotech, Nanjing, China), a sequencing library was generated using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA) and sequenced on an Illumina Novaseq6000 sequencing system at Berry Genomics Corporation (Beijing, China). A total of 6,899,143 high-quality clean reads were obtained after trimming and used for contig assembly. BLASTn and BLASTx analyses on the contigs (n = 128,400) showed that one contig (9,245 bp in length) exhibited a sequence identity of 84.0% with the reference sequence of dasheen mosaic virus (DsMV, NCBI reference seq. NC_003537, genus Potyvirus, family Potyviridae) , suggesting infection of the plants by DsMV. No other viral sequences were detected in the sample. To confirm these results, a near full-length genomic sequence of DsMV was obtained from one sample (sample no. 39) by reverse transcription polymerase chain reaction (RT-PCR) of three overlapping fragments with the following primer pairs: DsMV-1F (5'-AAATTAAAACATCTCAACAAAACCTACA-3') /DsMV-4130R (5'-TTCATGGTCCTCGTGGAGTATA-3'), DsMV-3870F (5'-GAGGACGTGAGAATTCAAAGTCT-3')/DsMV-8250R (5'-GTCCAACCTTGCTTGATGCATGC-3'), DsMV-7690F (5'-GGAGCGACTCCTCTTCCAAAGTTGTG-3')/DsMV-10100R (5'-TGAACACCGTGCACGAAGCATCTC-3'). The PCR products were cloned into pMD19-T vector (TAKARA Biotech, Dalian, China) and sequenced. The near full-length genomic sequence of the isolate (DsMV-BF39) was 9,737 nt in length and deposited into GenBank under the accession no. MZ043618. BLASTn analysis of this sequence demonstrated that it shared an identity ranging from 78.6% (MG602234) to 85.6% (MG602227) with various DsMV isolates. To determine whether DsMV was closely associated with the symptoms observed in T. giganteum Engl., leaf tissues from 30 symptomatic plants and 22 asymptomatic plants were analyzed by RT-PCR using primer pairs DsMV-CPF (5'-TGTTCTGTGAACATGATGAAGTTG-3', sense) and DsMV-CPR (5'-GTAACTGTGGCCTGTTTACCAG-3', antisense) targeting a 916 bp fragment of the CP gene of DsMV. Amplicons with the expected size were detected from the 30 symptomatic plants but not from the 22 asymptomatic plants, suggesting a close association between DsMV infection and the observed symptoms. To our knowledge, this is the first report of DsMV infecting T. giganteum Engl.. Further study is needed to identify the specific symptoms induced by this virus in T. giganteum Engl. and to understand the biological characteristics, epidemiology, prevalence of this virus in China.
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http://dx.doi.org/10.1094/PDIS-03-21-0589-PDNDOI Listing
August 2021

A Simple Gas-Solid Treatment for Surface Modification of Li-Rich Oxides Cathodes.

Angew Chem Int Ed Engl 2021 Aug 18. Epub 2021 Aug 18.

Department of Chemical Engineering, University of Sichuan, Chengdu, 610065, P. R. China.

Li-rich layered oxides with high capacity are expected to be the next generation of cathode materials. However, the irreversible and sluggish anionic redox reaction leads to the O loss in the surface as well as the capacity and voltage fading. In the present study, a simple gas-solid treatment with ferrous oxalate has been proposed to uniformly coat a thin spinel phase layer with oxygen vacancy and simultaneously realize Fe-ion substitution in the surface. The integration of oxygen vacancy and spinel phase suppresses irreversible O release, prevents electrolyte corrosion, and promotes Li-ion diffusion. In addition, the surface doping of Fe-ion can further stabilize the structure. Accordingly, the treated Feox-2 % cathode exhibits superior capacity retention of 86.4 % and 85.5 % at 1 C and 2 C to that (75.3 % and 75.0 %) of the pristine sample after 300 cycles, respectively. Then, the voltage fading is significantly suppressed to 0.0011 V per cycle at 2 C especially. The encouraging results may play a significant role in paving the practical application of Li-rich layered oxides cathode.
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http://dx.doi.org/10.1002/anie.202107955DOI Listing
August 2021

Research on preparation and in vitro evaluation of the dendrimer-peptide nuclear acid conjugate for amplification pretargeting.

J Labelled Comp Radiopharm 2021 Sep 10;64(11):428-439. Epub 2021 Aug 10.

Radiopharmaceuticals Center, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, China.

Amplification pretargeting has the potential to increase the tracer's accumulation in the tumor. This study aimed to develop a three-step amplification pretargeting strategy in nuclear medicine with a polymer conjugated with multiple copies of peptide nuclear acid (PNA). In this study, the tracer F-labeled complementary PNA ( F-cPNA) was prepared by click-chemistry with high radiochemical purity (>99%) and great stability in vitro. The PAMMA dendrimer generation 4 (G4) was conjugated with multiple copies of PNAs. The average number of PNA groups in the G4-PNA conjugate was determined by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and the accessibility to the F-cPNA was identified by size-exclusion high-performance liquid chromatography (SE-HPLC). There were approximately 11.7 of 64 carboxyl groups modified with PNAs, of which more than 99% were accessible to F-cPNA. F-cPNA was added to a mixture of CC49-cPNA and G4-PNA, and the complex exhibited a single peak on high-performance liquid chromatography (HPLC) as evidence of complete hybridization between F-cPNA and CC49-cPNA/G4-PNA. The LS174T tumor cells were incubated with CC49-cPNA followed by G4-PNA as an amplification platform before F-cPNA was added to hybridize with CC49-cPNA/G4-PNA. Compared with conventional pretargeting without G4-PNA, the radioactivity signal was amplified about four times, which demonstrated that the dendrimer-PNA conjugate plays a crucial role in signal amplification.
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http://dx.doi.org/10.1002/jlcr.3937DOI Listing
September 2021

Insights into molecular structure, genome evolution and phylogenetic implication through mitochondrial genome sequence of Gleditsia sinensis.

Sci Rep 2021 Jul 21;11(1):14850. Epub 2021 Jul 21.

State Key Laboratory of Component-Based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China.

Gleditsia sinensis is an endemic species widely distributed in China with high economic and medicinal value. To explore the genomic evolution and phylogenetic relationships of G. sinensis, the complete mitochondrial (mt) genome of G. sinensis was sequenced and assembled, which was firstly reported in Gleditsia. The mt genome was circular and 594,121 bp in length, including 37 protein-coding genes (PCGs), 19 transfer RNA (tRNA) genes and 3 ribosomal RNA (rRNA) genes. The overall base composition of the G. sinensis mt genome was 27.4% for A, 27.4% for T, 22.6% for G, 22.7% for C. The comparative analysis of PCGs in Fabaceae species showed that most of the ribosomal protein genes and succinate dehydrogenase genes were lost. In addition, we found that the rps4 gene was only lost in G. sinensis, whereas it was retained in other Fabaceae species. The phylogenetic analysis based on shared PCGs of 24 species (22 Fabaceae and 2 Solanaceae) showed that G. sinensis is evolutionarily closer to Senna species. In general, this research will provide valuable information for the evolution of G. sinensis and provide insight into the phylogenetic relationships within the family Fabaceae.
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http://dx.doi.org/10.1038/s41598-021-93480-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8295344PMC
July 2021

Epigenetic heterogeneity promotes acquired resistance to BET bromodomain inhibition in ovarian cancer.

Am J Cancer Res 2021 15;11(6):3021-3038. Epub 2021 Jun 15.

State Key Laboratory of Oncogenes and Related Genes, Department of Obstetrics and Gynecology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University Shanghai, China.

BET bromodomain inhibitors (BETi) are promising therapeutic regimens for epithelial ovarian cancer (EOC). However, early-stage clinical trials indicate that drug tolerance may limit their anti-tumor efficacy. Here, we show that JQ1-refractory EOC cells acquire reversible resistance to BET inhibition and remain dependent on BRD4 function. The insensitivity is driven by a unique non-genetic mechanism that involves clonal selection for a pre-existing cell subpopulation with ample acetylated histones and sufficient nuclear phase-separated BRD4 droplets to counteract BETi antagonism. A vertical combination approach by co-blocking BET proteins and downstream Aurora kinases proves to achieve more complete responses than single inhibitors. Collectively, our study implicates epigenetic heterogeneity in therapeutic resistance to chromatin-targeted agents and proposes a rational strategy to address this anticipated clinical dilemma.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8263684PMC
June 2021

Leveraging 16S rRNA Microbiome Sequencing Data to Identify Bacterial Signatures for Irritable Bowel Syndrome.

Front Cell Infect Microbiol 2021 11;11:645951. Epub 2021 Jun 11.

State Key Laboratory of Component-Based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China.

Irritable bowel syndrome (IBS) is a chronic gastrointestinal disorder characterized by abdominal pain or discomfort. Previous studies have illustrated that the gut microbiota might play a critical role in IBS, but the conclusions of these studies, based on various methods, were almost impossible to compare, and reproducible microorganism signatures were still in question. To cope with this problem, previously published 16S rRNA gene sequencing data from 439 fecal samples, including 253 IBS samples and 186 control samples, were collected and processed with a uniform bioinformatic pipeline. Although we found no significant differences in community structures between IBS and healthy controls at the amplicon sequence variants (ASV) level, machine learning (ML) approaches enabled us to discriminate IBS from healthy controls at genus level. Linear discriminant analysis effect size (LEfSe) analysis was subsequently used to seek out 97 biomarkers across all studies. Then, we quantified the standardized mean difference (SMDs) for all significant genera identified by LEfSe and ML approaches. Pooled results showed that the SMDs of nine genera had statistical significance, in which the abundance of , and in IBS were higher, while the dominant abundance genera of healthy controls were Ruminococcaceae , , , and . In summary, based on six published studies, this study identified nine new microbiome biomarkers of IBS, which might be a basis for understanding the key gut microbes associated with IBS, and could be used as potential targets for microbiome-based diagnostics and therapeutics.
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http://dx.doi.org/10.3389/fcimb.2021.645951DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8231010PMC
July 2021

Agricultural cooperatives participating in vegetable supply chain integration: A case study of a trinity cooperative in China.

PLoS One 2021 24;16(6):e0253668. Epub 2021 Jun 24.

Wenzhounese Economy Research Institute, Wenzhou, Zhejiang, P.R.China.

Supply chain integration plays an important role in the development of the vegetable industry in terms of vegetable quality, vegetable safety, and vegetable security in rural China. This paper explores how agricultural cooperatives integrate the vegetable supply chain by taking a trinity cooperative as an example in China. It explains the translation concatenation of supply chain integration for this cooperative by constructing actor networks in four development stages, including the seed stage, start-up stage, development stage, and mature stage. The findings show that supply chain integration in production cooperation, supply & sales cooperation, and credit cooperation is a useful trinity cooperative model of supply chain integration for investigating vegetable supply chain integration through internal integration and external integration. This paper suggests that cooperatives in the vegetable supply chain should facilitate close coordination among different shareholders and further improve the efficiency of supply chain integration. The government should provide training opportunities and funding to encourage cooperatives to participate in supply chain integration within the vegetable industry.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0253668PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8224856PMC
June 2021

Role of lung and gut microbiota on lung cancer pathogenesis.

J Cancer Res Clin Oncol 2021 Aug 20;147(8):2177-2186. Epub 2021 May 20.

Qingdao Hospital of Traditional Chinese Medicine (Qingdao Hiser Hospital), Qingdao, 266000, China.

Background: Lung cancer is the leading cause of cancer-related deaths worldwide (Ferlay et al., Int J Cancer 136:E359-386, 2015). In addition, lung cancer is associated with the highest mortality among all cancer types (Wu et al., Exp Ther Med 16:3004-3010, 2018). Previous studies report that microbiota play an important role in lung cancer. Notably, changes in lung and gut microbiota, are associated with progression of lung cancer. Several studies report that lung and gut microbiome promote lung cancer initiation and development by modulating metabolic pathways, inhibiting the function of immune cells, and producing pro-inflammatory factors. In addition, some factors such as microbiota dysbiosis, affect production of bacteriotoxins, genotoxicity and virulence effect, therefore, they play a key role in cancer progression. These findings imply that lung and gut microbiome are potential markers and targets for lung cancer. However, the role of microbiota in development and progression of lung cancer has not been fully explored.

Purpose: The aim of this study was to systemically review recent research findings on relationship of lung and gut microbiota with lung cancer. In addition, we explored gut-lung axis and potential mechanisms of lung and gut microbiota in modulating lung cancer progression.

Conclusion: Pulmonary and intestinal flora influence the occurrence, development, treatment and prognosis of lung cancer, and will provide novel strategies for prevention, diagnosis, and treatment of lung cancer.
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http://dx.doi.org/10.1007/s00432-021-03644-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8236441PMC
August 2021

The complete mitochondrial genome sequence of (Gerromorpha: Gerridae).

Mitochondrial DNA B Resour 2021 Apr 22;6(4):1471-1472. Epub 2021 Apr 22.

State Key Laboratory of Component-Based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China.

In this study, the complete mitochondrial genome of was sequenced and assembled, which was first reported in . The mitogenome of was 15,391 bp in length, and it contained 22 transfer RNA (tRNA) genes, two ribosomal RNA (rRNA) genes, 13 protein-coding genes (PCGs), and a control region (D-loop), the overall base nucleotide compositions encoded was 42.9% A, 14.3% C, 10.0% G, and 32.8% T.
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http://dx.doi.org/10.1080/23802359.2021.1909436DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8079053PMC
April 2021

Stimuli-Responsive Memristive Materials for Artificial Synapses and Neuromorphic Computing.

Adv Mater 2021 Apr 9:e2006469. Epub 2021 Apr 9.

Department of Chemistry, National University of Singapore, Singapore, 117543, Singapore.

Neuromorphic computing holds promise for building next-generation intelligent systems in a more energy-efficient way than the conventional von Neumann computing architecture. Memristive hardware, which mimics biological neurons and synapses, offers high-speed operation and low power consumption, enabling energy- and area-efficient, brain-inspired computing. Here, recent advances in memristive materials and strategies that emulate synaptic functions for neuromorphic computing are highlighted. The working principles and characteristics of biological neurons and synapses, which can be mimicked by memristive devices, are presented. Besides device structures and operation with different external stimuli such as electric, magnetic, and optical fields, how memristive materials with a rich variety of underlying physical mechanisms can allow fast, reliable, and low-power neuromorphic applications is also discussed. Finally, device requirements are examined and a perspective on challenges in developing memristive materials for device engineering and computing science is given.
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http://dx.doi.org/10.1002/adma.202006469DOI Listing
April 2021

Deriving Soil Quality Criteria of Chromium Based on Species Sensitivity Distribution Methodology.

Toxics 2021 Mar 16;9(3). Epub 2021 Mar 16.

Department of Earth and Atmospheric Sciences, University of Alberta, Edmonton, AB T6G 2E3, Canada.

Chromium (Cr) is one of the most severe heavy metal contaminants in soil, and it seriously threatens ecosystems and human health through the food chain. It is fundamental to collect toxicity data of Cr before developing soil quality criteria/standards in order to efficiently prevent health risks. In this work, the short-term toxic effects of Cr(VI) and Cr(III) on the root growth of eleven terrestrial plants were investigated. The corresponding fifth percentile hazardous concentrations (HC) by the best fitting species sensitivity distribution (SSD) curves based on the tenth percentile effect concentrations (EC) were determined to be 0.60 and 4.51 mg/kg for Cr (VI) and Cr (III), respectively. Compared to the screening level values worldwide, the HC values in this study were higher for Cr(VI) and lower for Cr(III) to some extent. The results provide useful toxicity data for deriving national or local soil quality criteria for trivalent and hexavalent Cr.
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http://dx.doi.org/10.3390/toxics9030058DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7998722PMC
March 2021

Inhibition of the shuttle effect of lithium-sulfur batteries a tannic acid-metal one-step chemical film-forming modified separator.

Nanoscale 2021 Mar;13(9):5058-5068

School of Chemical Engineering, Sichuan University, No. 24 South Section 1, Yihuan Road, Chengdu, 610065, PR China.

The dissolution of polysulfides in an electrolyte is a thermodynamically favorable process, which in theory means that the shuttle effect in lithium-sulfur batteries (LSBs) cannot be completely suppressed. So, it is very important to modify the separator to prevent the migration of polysulfides to the lithium anode. The traditional coating modification process of the separator is cumbersome and uses a solvent that is harmful to the environment, and too many inactive components affect the overall energy density of the battery. It is thus imperative to find a simple and environmentally friendly modification process of the separator. In this study, a fast chemical film-forming method is proposed to modify the separator of a lithium-sulfur battery using tannic acid (TA) and cobalt ions (Co2+). This method requires only simple steps and environmentally friendly raw materials to obtain a thin coating (only 5.83 nm) that can effectively inhibit the shuttle effect. The lithium-sulfur battery with the TA-Co separator shows superior long cycle performance. After 500 cycles at 0.5 C, the capacity decay rate of each cycle is only 0.065%. On the other hand, the TA-Co separator can inhibit the growth of lithium dendrites and help to build a stable lithium anode, which can exhibit minimal polarization (56 mV) in a lithium-lithium symmetrical battery at the current density of 2 mA cm-2. The rapid and simple modification method proposed in this study has a certain reference value for the future large-scale application of lithium sulfur batteries.
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http://dx.doi.org/10.1039/d1nr00034aDOI Listing
March 2021

A Ge/Carbon Atomic-Scale Hybrid Anode Material: A Micro-Nano Gradient Porous Structure with High Cycling Stability.

Angew Chem Int Ed Engl 2021 May 26;60(22):12539-12546. Epub 2021 Apr 26.

College of Chemical Engineering, Sichuan University, Chengdu, 610065, P. R. China.

The continuous growth of the solid-electrolyte interface (SEI) and material crushing are the fundamental issues that hinder the application of Ge anodes in lithium-ion batteries. Solving Ge deformation crushing during discharge/charge cycles is challenging using conventional carbon coating modification methods. Due to the chemical stability and high melting point of carbon (3500 °C), Ge/carbon hybridization at the atomic level is challenging. By selecting a suitable carbon source and introducing an active medium, we have achieved the Ge/carbon doping at the atom-level, and this Ge/carbon anode shows excellent electrochemical performance. The reversible capacity is maintained at 1127 mAh g after 1000 cycles (2 A g (2-71 cycles), 4 A g (72-1000 cycles)) with a retention of 84 % compared to the second cycle. The thickness of the SEI is only 17.4 nm after 1000 cycles. The excellent electrochemical performance and stable SEI fully reflect the application potential of this material.
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http://dx.doi.org/10.1002/anie.202102048DOI Listing
May 2021

Theoretical evaluation of the carbene-based site-selectivity in gold(III)-catalyzed annulations of alkynes with anthranils.

Chem Commun (Camb) 2021 Feb;57(12):1494-1497

School of Chemistry and Chemical Engineering, Qufu Normal University, Qufu, 273165, P. R. China.

The gold(iii)-catalyzed annulations of alkynes with anthranils were evaluated using DFT calculations. A unified rationale for the Br-migration on α-imino gold(iii)-carbene was proposed, from which an unprecedented "N-donation/abstraction substitution" mechanism was established using the substituted anthranils, while direct C-H nucleophilic attack was involved with the unsubstituted anthranils. The controlling factors guiding the site-selectivity were uncovered. These computational studies provide insight for developing new α-imino gold(iii)-carbene mediated reactions.
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http://dx.doi.org/10.1039/d0cc07440cDOI Listing
February 2021

First report of Fusarium proliferatum causing root rot disease in Salvia miltiorrhizae in China.

Plant Dis 2020 Dec 15. Epub 2020 Dec 15.

Henan Academy of Agricultural Sciences, 74728, Institute of Plant Protection, Zhengzhou, Henan, China;

Salvia miltiorrhiza Bunge is a herb plant used as a traditional Chinese medicine to cure cardiovascular disease. In December 2018, a root rot disease was observed on S. miltiorrhiza in four surveyed counties (Song, Yuzhou, Fangcheng, and Mianchi) in Henan province in China. The disease incidence ranged from 15 to 50% in 12 surveyed fields. At the early stage, the diseased plants were wilting with purple leaves. Leaves and branches became withered and fibrous roots became brown and rotted. The main roots of severely diseased plants also became rotted. The color of the stem surface turned from red to black, and the color of the stem xylem and phloem turned from dark red to brown. Eventually, the roots of diseased plants became completely rotted and the whole plants became dead, but no stink, which is different from Fusarium solani (Mart.) Sacc. (Yuan et al. 2015). Diseased root tissues (5×5×5 mm in size) were cut from diseased plants, surface-sterilized with 1% sodium hypochlorite for 1 min followed by dipping in 75% alcohol for 30 sec, rinsed in sterile distilled water for 3 times, air-dried on a sterilized filter paper in a laminar flow hood, placed on potato dextrose agar (PDA) containing 250 mg/l of streptomycin sulfate, and incubated at 28℃. Five isolates of Fusarium were obtained and purified using the single-spore isolation method. On PDA plates, the colonies were purple in color with formation of white aerial mycelia and reached 50 to 60 mm in diameter after incubation for 5 days. The colonies produced abundant microconidia on the colonies. The microconidia were 4.3 to 12.3 (10.0) × 2.1 to 3.5 (3.1) μm in size (n = 40), hyaline, ovoid or ellipse in shape. The conidiogenous cells were polyphialides. On mung bean media, the isolates formed macroconidia with 3 to 6 septae, fusiform in shape, slightly curved, 21.8 to 32.7 (31.4) × 2.6 to 4.3 (3.4) μm in size (n = 50). The morphological features of the five isolates were consistent with the description for Fusarium proliferatum (Matsush.) Nirenberg ex Gerlach & Nirenberg (Leslie and Summerell 2006). To further define the identity of the five isolates, molecular phylogenetic analysis was performed. The genomic DNA was extracted from all five isolates using the cetyl trimethylammonium bromide (CTAB) method. Five genes [nuclear ribosomal internal transcribed spacer (ITS) region, translation elongation factor 1-α (EF1α), β-tubulin gene, partial sequence for calmodulin (PRO), and RNA-dependent DNA polymerase II subunit (RPB2)] in F. proliferatum were amplified using primers pairs ITS1/ITS4, EF1T/2T, β-tubulin 2a/b, PRO1/2, and RPB2F/R, respectively (Glass and Donaldson 1995; Liu et al. 1999; Mulè 2004; O'Donnell et al. 1998; O'Donnell et al., 2010). The sequences (GenBank accession numbers: MT371373, MT371384, MT925651, MT925652, and MT934441, respectively) showed 99.6 to 100% identities to the corresponding DNA sequences in F. proliferatum (GenBank Acc. Nos. MK243486, MN245720, KJ12896, MN245721, and MK144327, respectively). All five isolates were tested for pathogenicity to fulfill the Koch's postulates. The 45-day-old healthy plants of S. miltiorrhiza grown in sterilized soil in pots (20 cm in diameter), one plant in one pot, were inoculated with conidial suspensions (1.0 × 107 cfu/ml) by pouring 10 ml conidial suspensions around the stem base in one pot. For each isolate, four plants were inoculated. Four plants were treated with sterilized water in the same volume as a control. The tested plants were placed in a growth room at 25°C (RH > 60%) with a 12 h photoperiod of fluorescent light. The pathogenicity assay was repeated for three times. The similar wilt symptoms were observed on the roots in the inoculated plants 30 days after inoculation but were not observed in the control plants. F. proliferatum was re-isolated from the infected roots, and its identity was confirmed by PCR with the primers described above. To our knowledge, this is the first report of F. proliferatum casing root rot disease on S. miltiorrhiza in China.
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http://dx.doi.org/10.1094/PDIS-09-20-1908-PDNDOI Listing
December 2020

Nickel-Rich Layered Cathode Materials for Lithium-Ion Batteries.

Chemistry 2021 Mar 18;27(13):4249-4269. Epub 2021 Jan 18.

Department of Chemical Engineering, University of Sichuan, Chengdu, 610065, P. R. China.

Nickel-rich layered transition metal oxides are considered as promising cathode candidates to construct next-generation lithium-ion batteries to satisfy the demands of electrical vehicles, because of the high energy density, low cost, and environment friendliness. However, some problems related to rate capability, structure stability, and safety still hamper their commercial application. In this Review, beginning with the relationships between the physicochemical properties and electrochemical performance, the underlying mechanisms of the capacity/voltage fade and the unstable structure of Ni-rich cathodes are deeply analyzed. Furthermore, the recent research progress of Ni-rich oxide cathode materials through element doping, surface modification, and structure tuning are summarized. Finally, this review concludes by discussing new insights to expand the field of Ni-rich oxides and promote practical applications.
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http://dx.doi.org/10.1002/chem.202003987DOI Listing
March 2021

Independent risk factors for lymph node metastasis in 2623 patients with Non-Small cell lung cancer.

Surg Oncol 2020 Sep 22;34:256-260. Epub 2020 May 22.

Department of Thoracic Surgery, The First Medical Centre, Chinese PLA General Hospital, Beijing, China. Electronic address:

Purpose: this study attempts to identify the independent risk factors that can predict lymph node metastasis for the patients with non-small cell lung cancer (NSCLC), and guide doctor adoption of individualized treatment for such patients.

Materials And Methods: This study was approved by the Hospital's Ethics Committee and all patients had signed informed consent forms. We retrospectively reviewed NSCLC patients who had undergone surgical resection from December 2008 to December 2013.The statistical significance of evaluation variables and lymph node metastasis was determined with Pearson's Chi-square test. The risk factors of lymph node metastasis were determined through univariate and multivariate logistic regression analysis. And for the age and tumor diameter factors, optimal cutoff points were determined with a receiver operating characteristic analysis.

Results: In the present study, a total of 2623 patients were included in the study, and 779 patients with lymph node metastasis. Three independent risk factors were identified: age, tumor diameter and Ki-67 index. We found that <65 years of age (Adjusted-OR:1.921), ≥2.85 cm of tumor diameter (Adjusted-OR:3.141), and 5%~25% in Ki-67 group (Adjusted-OR:2.137),≥25% (Adjusted-OR:3.341) were significant. Also we found that 307 patients with lymph node metastasis and the lymph node metastasis rate was 51.0%, when the age<65 years, Ki-67 index≥25%, and the tumor diameter≥2.85 cm. On the contrary, there were only 2 patients with lymph node metastasis, and the rate of lymph node metastasis was 5.1%.

Conclusion: Identifying three independent risk factors that predict lymph node metastasis in non-small cell patients, Among NSCLC patients in whom all three predictors were identified, and over a half of the patients showed lymph node metastasis.
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http://dx.doi.org/10.1016/j.suronc.2020.05.005DOI Listing
September 2020

Long Non-Coding RNA AGAP2-AS1/miR-628-5p/PTN Axis Modulates Proliferation, Migration, Invasion, and Apoptosis of Glioma Cells.

Cancer Manag Res 2020 20;12:6059-6068. Epub 2020 Jul 20.

Department of Neurosurgery, The Fifth Affiliated Hospital Sun Yat-Sen University, Zhuhai, Guangdong, People's Republic of China.

Purpose: Long non-coding RNAs (lncRNAs) have been reported to be involved in a variety of cancers, including glioma. However, the exact role and underlying mechanism of lncRNA AGAP2 antisense RNA 1 (AGAP2-AS1) in glioma have not yet been fully elucidated.

Methods: The expression levels of AGAP2-AS1, microRNA-628-5p (miR-628-5p) and pleiotrophin (PTN) were measured by quantitative real-time polymerase chain reaction (qRT-PCR). Cell proliferation, apoptosis, migration and invasion were detected by Cell Counting Kit-8 (CCK-8) assay, flow cytometry, transwell assay, respectively. Western blot assay was used to detect the protein level of PTN. The interaction between miR-628-5p and AGAP2-AS1 or PTN was predicted by bioinformatics software and confirmed by the dual-luciferase reporter and RNA Immunoprecipitation (RIP) assays. Murine xenograft model was established to confirm the role of AGAP2-AS1 in glioma progression in vivo.

Results: AGAP2-AS1 expression was upregulated in glioma tissues and cells. Knockdown of AGAP2-AS1 inhibited the proliferation, migration and invasion, but facilitated apoptosis in glioma cells. Moreover, AGAP2-AS1 could directly bind to miR-628-5p and its overexpression reversed the anti-tumor effect of miR-628-5p restoration on the progression of glioma cells. In addition, miR-628-5p directly targeted PTN and its inhibition abolished the inhibitory effect of PTN knockdown on the progression of glioma cells. Furthermore, AGAP2-AS1 functioned as a competing endogenous RNA (ceRNA) by sponging miR-628-5p to modulate PTN expression. Besides, AGAP2-AS1 depletion reduced tumor growth by upregulating miR-628-5p and downregulating PTN.

Conclusion: AGAP2-AS1 knockdown suppressed cell proliferation, migration and invasion but promoted cell apoptosis in glioma cells by regulating miR-628-5p/PTN axis, providing novel avenues for treatment of glioma.
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http://dx.doi.org/10.2147/CMAR.S250890DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7398883PMC
July 2020

Targeting NK Cell Checkpoint Receptors or Molecules for Cancer Immunotherapy.

Authors:
Cai Zhang Yuxia Liu

Front Immunol 2020 23;11:1295. Epub 2020 Jun 23.

Institute of Immunopharmaceutical Sciences, School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, China.

Checkpoint blockade therapy, for example using antibodies against CTLA-4 and PD-1/PD-L1, relieves T cells from the suppression by inhibitory checkpoints in the tumor microenvironment; thereby achieving good outcomes in the treatment of different cancer types. Like T cells, natural killer (NK) cell inhibitory receptors function as checkpoints for NK cell activation. Upon interaction with their cognate ligands on infected cells, tumor cells, dendritic cells and regulatory T cells, signals from these receptors severely affect NK cells' activation and effector functions, resulting in NK cell exhaustion. Checkpoint inhibition with antagonistic antibodies (Abs) can rescue NK cell exhaustion and arouse their robust anti-tumor capacity. Most notably, the response to anti-PD-1 therapy can be enhanced by the increased frequency and activation of NK cells, thereby increasing the overall survival of patients with multiple types of cancer. In addition, rescue of NK cell activity could enhance adaptive T cells' anti-tumor activity. Some antagonistic Abs (e.g., anti-TIGIT and anti-NKG2A monoclonal Abs) have extraordinary potential in cancer therapy, as evidenced by their induction of potent anti-tumor immunity through recovering both NK and T cell function. In this review, we summarize the dysfunction of NK cells in the tumor microenvironment and the key NK cell checkpoint receptors or molecules that control NK cell function. We particularly focus on recent advances in the most promising strategies through blockade of NK cell checkpoints or their combination with other approaches to more effectively reject tumors.
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http://dx.doi.org/10.3389/fimmu.2020.01295DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7344328PMC
April 2021

Strategy for Isolation, Preparation, and Structural Analysis of Chondroitin Sulfate Oligosaccharides from Natural Sources.

Anal Chem 2020 09 10;92(17):11644-11653. Epub 2020 Aug 10.

College of Life Science, Northwest University, Xi'an 710069, China.

The structure of chondroitin sulfate oligosaccharides (CSOs), especially their sulfation pattern, has been found to be closely related with many biological pathways and diseases. However, detailed functional analysis such as their interaction with glycan binding proteins (GBPs) has been lagging, presumably due to the unavailability of well-defined, diverse structures. Besides challenging chemical and enzymatic synthesis, this is also due to the challenges in their purification at the isomer level and structural analysis owing to their instability, structural complexity, and low mass spectrometry detection sensitivity. Herein, we first used recycling preparative HPLC to separate and purify shark CS tetrasaccharide component labeled by a bifunctional fluorescent linker 2-amino--(2-aminoethyl)benzamide (AEAB) at the isomer level. Then, each isomer was derivatized through a multistage procedure including -acetylation, carboxyl amidation, permethylation, and desulfation with silylating reagent. Structural analysis of each derivatized isomer was performed with ESI-MS in positive ion mode. A total of 16 isomers of CSO-AEAB were isolated, with a minimum mass component of 0.007 mg and a maximum mass component of 17.53 mg, of which 10 isomers (>90 μg) were structurally analyzed. This preparation and structure analysis of CSOs lay the foundation for further study of the structure-activity relationship of CSOs.
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http://dx.doi.org/10.1021/acs.analchem.0c01410DOI Listing
September 2020

Density Functional Theory Study on the Mechanism of Iridium-Catalyzed Benzylamine C-H Alkenylation with Ethyl Acrylate.

ACS Omega 2020 Jun 15;5(25):15446-15453. Epub 2020 Jun 15.

School of Chemistry and Chemical Engineering, Qufu Normal University, Qufu, Shandong 273165, China.

Iridium-catalyzed oxidative o-alkenylation of benzylamines with acrylates was enabled by the directing group pentafluorobenzoyl (PFB). Density functional theory calculations were performed to explore the detailed reaction mechanism. The calculated results reveal that N-deprotonation prior to C-H activation is favored over direct C-H activation. Moreover, C-H activation is reversible and not the rate-determining step, which has been supported by the experimental observation. The regio- and stereoselectivity of ethyl acrylate insertion are controlled by the steric effect and the carbon atom with a larger orbital coefficient of the π* antibonding orbital in the nucleophilic attack, respectively. The migratory insertion of ethyl acrylate is computationally found to be rate-determining for the whole catalytic cycle. Finally, the seven-membered ring intermediate undergoes a sequential N-protonation and β-H elimination with the assistance of AcOH, rather than β-H elimination and reductive elimination proposed experimentally, to afford the o-alkenylated product. is unable to directly cyclize through C-N reductive elimination because both sp-hybridized N and C atoms are unfavorable for N-C reductive elimination. The origin of the directing group PFB preventing the product and intermediates undergoing aza-Michael addition has been explained.
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http://dx.doi.org/10.1021/acsomega.0c01587DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7331057PMC
June 2020

Competitive adsorption of heavy metals by anaerobic ammonium-oxidizing (anammox) consortia.

Chemosphere 2020 Nov 6;258:127289. Epub 2020 Jun 6.

State Key Laboratory of Water and Sediment Sciences (Ministry of Education), College of Environmental Science & Engineering, Peking University, Beijing, 100871, China. Electronic address:

Anammox-based processes and microbial consortia have drawn extensive attention for their use in high-efficiency wastewater treatment technologies. Metals substantially affect the activity of anammox consortia and the quality of wastewater treatment plant effluent. Here, we explored the role of anammox consortia in terms of metals complexation in both single and multi-metal systems. Adsorption edges of single metal cations indicate that the adsorption preference was in the order: Pb(II) > Cd(II) > Cr(VI). A competitive effect was observed in multi-metal cations systems, with Pb(II) being preferably adsorbed and the degree of adsorption somewhat reduced in the presence of either Cd(II) or Cr(VI), while Cd(II) and Cr(VI) were easily exchanged and substituted by other metals. Fourier transform infrared (FTIR) and X-ray photoelectron spectroscopy (XPS) further suggest that the adsorption of Pb(II) and Cd(II) are as inner-sphere ion-exchange mechanisms, while Cr(VI) adsorption is mainly by outer-sphere complexation. Density functional theory (DFT) calculations highlight that Cd(II) and Pb(II) have different binding sites compared to Cr(VI), and the order of binding energy (E) of three metal cations were Pb(II) > Cd(II) > Cr(VI). These calculations support the adsorption data in that Pb forms more stable complexes with anammox bacterial surface ligands. Surface complexation modelling (SCM) further predicted both the sorption of single metal cations and competitive adsorption of the three metals to anammox consortia, the exception being Cd at higher loadings. The results of this study highlight the potential role of anammox consortia in removing metal cations from wastewater in treatment systems.
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http://dx.doi.org/10.1016/j.chemosphere.2020.127289DOI Listing
November 2020

Preparation, structural characterization, and bioactivity of PHPD-IV-4 derived from Porphyra haitanensis.

Food Chem 2020 Nov 22;329:127042. Epub 2020 May 22.

Glycobiology and Glycotechnology Research Center, College of Food Science and Technology, Northwest University, Xi'an 710069, PR China. Electronic address:

Polysaccharides are a major active component of Porphyra haitanensis, which is an important food source in many countries. Four different molecular-weight fractions, namely PHPD-I (329 kDa), PHPD-II (203 kDa), PHPD-III (128 kDa), and PHPD-IV (10 kDa), were obtained from P. haitanensis polysaccharides by degradation using the HO/ascorbic acid system. PHPD-IV elicited the highest level of antioxidant and immunostimulatory activity among the four fractions. PHPD-IV was purified by DEAE-cellulose column and five fractions were obtained, designated PHPD-IV-1-PHPD-IV-5. PHPD-IV-4 displayed the greatest biological activity by up-regulating the phosphorylation of MAPK signalling molecules. PHPD-IV-4 was further purified, and its structure was characterized by monosaccharide composition and 1/2D-NMR analysis. The result revealed that PHPD-IV-4 was repeated units of → 3) β-d-galactose (1 → 4) 3, 6-anhydro-α-l-galactose (1→, and → 3) β-d-galactose (1 → 4) α-l-galactose-6-S (1→. This study provides a theoretical basis for the utilisation and structure-activity assessment of P. haitanensis polysaccharides.
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http://dx.doi.org/10.1016/j.foodchem.2020.127042DOI Listing
November 2020

Synergistic effect of uniform lattice cation/anion doping to improve structural and electrochemical performance stability for Li-rich cathode materials.

Nanotechnology 2020 Nov 21;31(45):455704. Epub 2020 May 21.

College of Chemical Engineering, Sichuan University, Chengdu 610065 People's Republic of China.

There has been extensive research into lithium-rich layered oxide materials as candidates for the nextgeneration of cathode materials in lithium-ion batteries, due to their high energy density and low cost; however, their poor cycle life and fast voltage fade hinder their large-scale commercial application. Here, we propose a novel cation/anion (Na/PO ) co-doping approach to mitigate the discharge capacity and voltage fade of a Co-free LiNiMnO cathode. Our results show that the synergistic effect of cation/anion doping can promote long cycle stability and rate performance by inhibiting the phase transformation of the layered structure to a spinel or rock-salt structure and stabilizing the well-ordered crystal structure during long cycles. The co-doped sample exhibits an outstanding cycle stability (capacity retention of 86.7% after 150 cycles at 1 C) and excellent rate performance (153 mAh g at 5 C). The large ionic radius of Na can expand the Li slab to accelerate Li diffusion and the large tetrahedral PO polyanions with high electronegativity stabilize the local structure to improve the electrochemical performance.
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http://dx.doi.org/10.1088/1361-6528/ab9579DOI Listing
November 2020

cake extracts reduce burn injury through suppressing inflammatory responses and enhancing collagen synthesis.

Food Nutr Res 2020 6;64. Epub 2020 Mar 6.

Center for Immunology, Key Laboratory of Prevention and Treatment of Cardiovascular and Cerebrovascular Diseases, Ministry of Education, Gannan Medical University, Ganzhou, Jiangxi, China.

Background: Burn injury accidents happen in our daily life, and the burn mortality is especially high in the low-to-middle-income countries. cake extracts (CCEs) are compound extracts from cake, and the major ingredients in CCEs may have antimicrobial, anti-oxidative, and anti-inflammatory effects. However, the effects of CCEs on burn inflammation and injury remain unknown.

Objective: This study is to investigate the effects of CCEs in burn injury and explore its mechanism.

Design: First, CCEs were identified to mainly contain camelliaside A and B using Ultra High Performance Liquid Chromatography-Time of Flight Mass Spectrometer (UHPLC-TOF-MS) method. Second, the CCEs' effect on burn was tested. Burn was induced by boiling water in mice, and then CCEs (30, 50, and 100 mg/mL) were applied on the damaged skin at 3, 7, and 14 days after burn induction.

Results: The results showed that CCEs protected the skin from burn-induced inflammation and enhanced the wound healing in a dose-dependent manner. CCEs decreased the expression levels of various cytokines including and , as well as inflammatory related factors iNOS. Moreover, CCEs increased the levels of collagens, including the mRNA of and , and inhibited the mRNA of and , and increased the collagen staining. CCEs also reversed the impairment of activity levels of anti-oxidative enzymes. Furthermore, CCEs suppressed the gene expression of pro-inflammatory cytokines in LPS-stimulated human skin keratinocyte, possibly through inhibiting NF-κB signaling pathway. In addition, toxicological safety experiments on CCEs showed that the oral median lethal dose (LD50) was 2,000 mg/kg, the percutaneous LD50 was greater than 2,000 mg/kg, and CCEs did not cause gene mutation.

Conclusion: CCEs exert a potent anti-inflammatory effect against burn damage in mice. And toxicological safety experiments suggest that CCEs are safe for usage.
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http://dx.doi.org/10.29219/fnr.v64.3782DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7217294PMC
March 2020

The Role of Human γδ T Cells in Anti-Tumor Immunity and Their Potential for Cancer Immunotherapy.

Authors:
Yuxia Liu Cai Zhang

Cells 2020 05 13;9(5). Epub 2020 May 13.

Institute of Immunopharmaceutical Sciences, School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, 44 Wenhua West Road, Jinan 250012, China.

γδ T cells are a distinct subset of T cells whose T cell receptors consist of γ chains and δ chains, different from conventional αβ T cells. γδ T cells are considered as a member of the innate immunity because of their non-MHC restricted antigen recognition, rapid response to invading pathogens and sense early changes of malignant cells. Upon activation, they can further promote the activation of adaptive immune cells, such as T cells and B cells, by secreting various cytokines. Thus, γδ T cells are regarded as a bridge between innate immunity and acquired immunity. γδ T cells are involved in a variety of immune response processes, including immune defense and immune surveillance against infection and tumorigenesis. γδ T cells recognize multiple tumor-associated antigens or molecules in T cell receptors (TCRs)-dependent and natural killer cell receptors (NKRs)-dependent ways. γδ T cells not only display a direct killing capacity on a variety of tumors, but also exert anti-tumor immune responses indirectly by facilitating the function of other immune cells, such as dendritic cells (DCs), B cells and CD8 T cells. In this review, we summarize the major subpopulations, the tumor recognition mechanisms, and the anti-tumor effects of human γδ T cells, particularly the potential of γδ T cells for cancer immunotherapy.
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http://dx.doi.org/10.3390/cells9051206DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7290839PMC
May 2020

Au Quantum Dot/Nickel Tetraminophthalocyanaine-Graphene Oxide-Based Photoelectrochemical Microsensor for Ultrasensitive Epinephrine Detection.

ACS Omega 2020 Apr 9;5(15):8423-8431. Epub 2020 Apr 9.

College of Chemistry and Chemical Engineering, Guangxi Normal University for Nationalities, Chongzuo 532200, China.

Owing to the importance of epinephrine as a neurotransmitter and hormone, sensitive methods are required for its detection. We have developed a sensitive photoelectrochemical (PEC) microsensor based on gold quantum dots (Au QDs) decorated on a nickel tetraminophthalocyanine-graphene oxide (NiTAPc-Gr) composite. NiTAPc was covalently attached to the surface of graphene oxide to prepare NiTAPc-Gr, which exhibits remarkable stability and PEC performance. In situ growth of Au QDs on the NiTAPc-Gr surface was achieved using chemical reduction at room temperature. The synthesized materials were characterized by Fourier transform infrared spectroscopy, ultraviolet-visible spectroscopy, X-ray photoelectron spectroscopy, scanning electron microscopy, transmission electron microscopy, and electrochemical impedance spectroscopy. Au [email protected] provided a much greater photocurrent than NiTAPc-Gr, making it suitable for the ultrasensitive PEC detection of epinephrine. The proposed PEC strategy exhibited a wide linear range of 0.12-243.9 nM with a low detection limit of 17.9 pM (S/N = 3). Additionally, the fabricated PEC sensor showed excellent sensitivity, remarkable stability, and good selectivity. This simple, fast, and low-cost strategy was successfully applied to the analysis of human serum samples, indicating the potential of this method for clinical detection applications.
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http://dx.doi.org/10.1021/acsomega.9b02998DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7178368PMC
April 2020
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