Publications by authors named "Yuting Zhang"

504 Publications

Differential expression analysis of mRNAs, lncRNAs, and miRNAs expression profiles and construction of ceRNA networks in PEDV infection.

BMC Genomics 2022 Aug 13;23(1):586. Epub 2022 Aug 13.

College of Veterinary Medicine, Northwest A&F University, Yangling, Xianyang, 712100, Shaanxi, China.

Background: Porcine Epidemic Diarrhea Virus (PEDV) is a coronavirus that seriously affects the swine industry. MicroRNAs and long noncoding RNAs are two relevant non-coding RNAs (ncRNAs) class and play crucial roles in a variety of physiological processes. Increased evidence indicates a complex interaction between mRNA and ncRNA. However, our understanding of the function of ncRNA involved in host-PEDV interaction is limited.

Results: A total of 1,197 mRNA transcripts, 539 lncRNA transcripts, and 208 miRNA transcripts were differentially regulated at 24 h and 48 h post-infection. Gene ontology (GO) and KEGG pathway enrichment analysis showed that DE mRNAs and DE lncRNAs were mainly involved in biosynthesis, innate immunity, and lipid metabolism. Moreover, we constructed a miRNA-mRNA-pathway network using bioinformatics, including 12 DE mRNAs, 120 DE miRNAs, and 11 pathways. Finally, the target genes of DE miRNAs were screened by bioinformatics, and we constructed immune-related lncRNA-miRNA-mRNA ceRNA networks. Then, the selected DE genes were validated by qRT-PCR, which were consistent with the results from RNA-Seq data.

Conclusions: This study provides the comprehensive analysis of the expression profiles of mRNAs, lncRNAs, and miRNAs during PEDV infection. We characterize the ceRNA networks which can provide new insights into the pathogenesis of PEDV.
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http://dx.doi.org/10.1186/s12864-022-08805-0DOI Listing
August 2022

Candle Soot-Based Electrosprayed Superhydrophobic Coatings for Self-Cleaning, Anti-Corrosion and Oil/Water Separation.

Materials (Basel) 2022 Aug 1;15(15). Epub 2022 Aug 1.

College of Mechanical Engineering and Automation, Huaqiao University, Xiamen 361021, China.

The interest in candle soot (CS)-based superhydrophobic coatings has grown rapidly in recent years. Here, a simple and low-cost process has been developed for the fabrication of CS-based superhydrophobic coatings through electrospraying of the composite cocktail solution of CS and polyvinylidene fluoride (PVDF). Results show that the superhydrophobicity of the coating closely relates to the loading amount of CS which results in coatings with different roughnesses. Specifically, increasing the CS amount (not more than 0.4 g) normally enhances the superhydrophobicity of the coating due to higher roughness being presented in the produced microspheres. Further experiments demonstrate that the superhydrophobicity induced in the electrosprayed coating results from the synergistic effect of the cocktail solution and electrospray process, indicating the importance of the coating technique and the solution used. Versatile applications of CS-based superhydrophobic coatings including self-cleaning, anti-corrosion and oil/water separation are demonstrated. The present work provides a convenient method for the fabrication of CS-based superhydrophobic coatings, which is believed to gain great interest in the future.
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http://dx.doi.org/10.3390/ma15155300DOI Listing
August 2022

Computational Analysis of Pathological Image Enables Interpretable Prediction for Microsatellite Instability.

Front Oncol 2022 22;12:825353. Epub 2022 Jul 22.

Department of Statistics and Finance/International Institute of Finance, School of Management, University of Science and Technology of China, Hefei, China.

Background: Microsatellite instability (MSI) is associated with several tumor types and has become increasingly vital in guiding patient treatment decisions; however, reasonably distinguishing MSI from its counterpart is challenging in clinical practice.

Methods: In this study, interpretable pathological image analysis strategies are established to help medical experts to identify MSI. The strategies only require ubiquitous hematoxylin and eosin-stained whole-slide images and perform well in the three cohorts collected from The Cancer Genome Atlas. Equipped with machine learning and image processing technique, intelligent models are established to diagnose MSI based on pathological images, providing the rationale of the decision in both image level and pathological feature level.

Findings: The strategies achieve two levels of interpretability. First, the image-level interpretability is achieved by generating localization heat maps of important regions based on deep learning. Second, the feature-level interpretability is attained through feature importance and pathological feature interaction analysis. Interestingly, from both the image-level and feature-level interpretability, color and texture characteristics, as well as their interaction, are shown to be mostly contributed to the MSI prediction.

Interpretation: The developed transparent machine learning pipeline is able to detect MSI efficiently and provide comprehensive clinical insights to pathologists. The comprehensible heat maps and features in the intelligent pipeline reflect extra- and intra-cellular acid-base balance shift in MSI tumor.
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http://dx.doi.org/10.3389/fonc.2022.825353DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355712PMC
July 2022

A surface-enhanced Raman scattering aptasensor for Escherichia coli detection based on high-performance 3D substrate and hot spot effect.

Anal Chim Acta 2022 Aug 1;1221:340141. Epub 2022 Jul 1.

The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.

A surface-enhanced Raman scattering (SERS) aptasensor was established for highly sensitive and selective detection of Escherichia coli (E. coli). Chitosan hydrogel modified with E. coli aptamer (Apt) functionalized silver nanoparticles was constructed as a SERS 3D substrate for specific bacteria enrichment, while the Raman signaling molecule 4-mercaptobenzoic acid and E. coli Apt modified gold nanostars were prepared for the sensitive quantification of E. coli. The aptasensor exhibits intense electromagnetic field enhancement in multiple hot spot regions, including the spikes and the gap between adjacent nanostars and that between gold nanostars and silver nanoparticles. Due to the hot spot effect coupled with the selective recognition ability, a detection limit of 3.46 CFU/mL with a wide dynamic linearized range from 3.2 × 10 to 3.2 × 10 CFU/mL could be achieved without other non-target bacteria interference. Moreover, this SERS aptasensor was applied to detect E. coli in actual samples with a good recovery rate (>90%). Therefore, the developed SERS aptasensor paves a new avenue for the detection in the field of food safety and environmental pollution by replacing the corresponding aptamers.
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http://dx.doi.org/10.1016/j.aca.2022.340141DOI Listing
August 2022

Corrigendum to "Mapping the environmental impacts and policy effectiveness of takeaway food industry in China" [Sci. Total Environ. 808 (2022) 152023].

Sci Total Environ 2022 Aug 4;848:157689. Epub 2022 Aug 4.

State Key Joint Laboratory of Environment Simulation and Pollution Control (SKLESPC), School of Environment, Tsinghua University, Beijing 100084, China; Industrial Energy Saving and Green Development Assessment Center, Tsinghua University, Beijing 100084, China. Electronic address:

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http://dx.doi.org/10.1016/j.scitotenv.2022.157689DOI Listing
August 2022

Genome Functional Analysis of the Psychrotrophic Lignin-Degrading Bacterium sp. C2 and the Role of DyP in Catalyzing Lignin Degradation.

Front Microbiol 2022 13;13:921549. Epub 2022 Jul 13.

College of Resources and Environment, Northeast Agricultural University, Harbin, China.

In the cold regions of China, lignin-rich corn straw accumulates at high levels due to low temperatures. The application of psychrotrophic lignin-degrading bacteria should be an effective means of overcoming the low-temperature limit for lignin degradation and promoting the utilization of corn straw. However, this application is limited by the lack of suitable strains for decomposition of lignin; furthermore, the metabolic mechanism of psychrotrophic lignin-degrading bacteria is unclear. Here, the whole genome of the psychrotrophic lignin-degrading bacterium sp. C2, isolated in our previous work, was sequenced. Comparative genomics revealed that C2 contained unique genes related to lignin degradation and low-temperature adaptability. DyP may participate in lignin degradation and may be a cold-adapted enzyme. Moreover, DyP was proven to catalyze lignin Cα-Cβ bond cleavage. Deletion and complementation of the gene verified its ability to catalyze the first-step reaction of lignin degradation. Comparative transcriptomic analysis revealed that the transcriptional expression of the gene was upregulated, and the genetic compensation mechanism allowed C2Δ to degrade lignin, which provided novel insights into the survival strategy of the psychrotrophic mutant strain C2Δ. This study improved our understanding of the metabolic mechanism of psychrotrophic lignin-degrading bacteria and provided potential application options for energy-saving production using cold-adapted lignin-degrading enzymes.
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http://dx.doi.org/10.3389/fmicb.2022.921549DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9327799PMC
July 2022

Activation, but not inhibition, of the indirect pathway disrupts choice rejection in a freely moving, multiple-choice foraging task.

Cell Rep 2022 Jul;40(4):111129

Department of Psychology, University of California, Berkeley, Berkeley, CA 94720, USA; Helen Wills Neuroscience Institute, University of California, Berkeley, Berkeley, CA 94720, USA. Electronic address:

The dorsomedial striatum (DMS) plays a key role in action selection, but less is known about how direct and indirect pathway spiny projection neurons (dSPNs and iSPNs, respectively) contribute to choice rejection in freely moving animals. Here, we use pathway-specific chemogenetic manipulation during a serial choice foraging task to test the role of dSPNs and iSPNs in learned choice rejection. We find that chemogenetic activation, but not inhibition, of iSPNs disrupts rejection of nonrewarded choices, contrary to predictions of a simple "select/suppress" heuristic. Our findings suggest that iSPNs' role in stopping and freezing does not extend in a simple fashion to choice rejection in an ethological, freely moving context. These data may provide insights critical for the successful design of interventions for addiction or other conditions in which it is desirable to strengthen choice rejection.
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http://dx.doi.org/10.1016/j.celrep.2022.111129DOI Listing
July 2022

Developing a two-stage auditory warning system for safe driving and eco-driving at signalized intersections: A driving simulation study.

Accid Anal Prev 2022 Jul 25;175:106777. Epub 2022 Jul 25.

MOT Key Laboratory of Transport Industry of Big Data Application Technologies for Comprehensive Transport, School of Traffic and Transportation, Beijing Jiaotong University, Beijing 100044, PR China.

In-vehicle intersection warning systems represent a promising approach for informing drivers of potential danger to reduce crashes and improve intersection safety. However, there is limited research on drivers' eco-driving performances, such as fuel consumption and emission, when drivers adapt their behaviors to the systems. In this study, an innovative two-stage in-vehicle intersection warning system was proposed to reduce red-light running (RLR) violations. Forty-five drivers participated in a simulated driving experiment and their driving performances at the intersections were evaluated to examine the effectiveness of the warning system. The measures included stop/go decision, RLR rate, average speed and deceleration, brake transition time, brake level, fuel consumption, and emission of CO and NOx. The results indicated that the warning system had a positive effect on drivers' safe driving and eco-driving performances, such as reducing the RLR rate, advancing and smoothing the deceleration and reducing fuel consumption and emission. Moreover, the impact of warning on drivers' performances varied with the time to the onset of yellow light. The research has practical implications for the automobile industry to use vehicle-to-infrastructure technology in the design of in-vehicle warning systems to improve driver behaviors at intersections.
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http://dx.doi.org/10.1016/j.aap.2022.106777DOI Listing
July 2022

Dietary flavonoids: a novel strategy for the intervention of cognitive impairment through intestinal microbiota.

J Sci Food Agric 2022 Jul 27. Epub 2022 Jul 27.

Department of Food Science and Engineering, Ningbo University, Ningbo, 315211, People's Republic of China.

The chances of people suffering from cognitive impairments have gradually increased with age. The diet and lifestyle are closely related to the occurrence and development of cognitive function, among which dietary flavonoid supplementation has been proved to be one of the protective factors of the cognitive function. Flavonoids belong to a class of polyphenols that have been proposed for the treatment of neurodegenerative diseases such as cognitive decline. Recent evidence has shown that intestinal flora in the human body can interact with flavonoids. Intestinal microbiota can modify the chemical structure of flavonoids, producing new metabolites that may be different from the pharmacological activities of the parent, meanwhile, flavonoids and their metabolites can in turn regulate the composition and structure of intestinal flora. Notably, intestinal flora affects the host nervous system activity through the gut-brain axis, causing changes in cognitive function ultimately. Therefore, this review summarized the interaction of dietary flavonoids and intestinal flora, as well as their protective effect on the cognitive decline through the gut-brain axis, which indicated that dietary flavonoids may ameliorate cognitive impairment through intestinal microbiota. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/jsfa.12151DOI Listing
July 2022

High Glucose Aggravates Retinal Endothelial Cell Dysfunction by Activating the RhoA/ROCK1/pMLC/Connexin43 Signaling Pathway.

Invest Ophthalmol Vis Sci 2022 Jul;63(8):22

Department of Ophthalmology, Qilu Hospital of Shandong University, Shandong University, Jinan, Shandong Province, China.

Purpose: This research aims to explore the mechanism underlying the relationship between RhoA/ROCK signaling and Connexin43 (Cx43) in retinal endothelial cell dysfunction and to evaluate the protective effect of ROCK inhibitors against retinal endothelial cell dysfunction in diabetic retinopathy (DR) models.

Methods: TUNEL staining, hematoxylin and eosin staining, a retinal digestion assay, and Evans blue assay were conducted to explore the effect of fasudil in alleviating retinal dysfunction induced by DR. ELISA, the CCK-8 assay, and flow cytometry were conducted to study inflammation, viability, and apoptosis of mouse retinal microvascular endothelial cells treated with high glucose and ROCK inhibitors. The qRT-PCR and Western blotting were used to evaluate the expression of RhoA, ROCK1, ROCK2, MLC, pMLC, and Cx43. Co-immunoprecipitation was used to verify the interaction between pMLC and Cx43. Immunofluorescence and scrape-loading and dye transfer were used to evaluate the expression and function of Cx43.

Results: Marked endothelial cell dysfunction resulting from the activation of RhoA/ROCK1 signaling was found in in vivo and in vitro models of DR. Via interaction with pMLC, which is downstream of RhoA/ROCK1, a significant downregulation of Cx43 was observed in retinal endothelial cells. Treatment with ROCK inhibitors ameliorated retinal endothelial dysfunction in vitro. The ROCK inhibitor, fasudil, significantly alleviated retinal dysfunction as shown by a decrease of retinal acellular capillaries, an improvement of vascular permeability, and a reduction of cell apoptosis in vivo.

Conclusions: Our study highlights a novel mechanism that high glucose could activate RhoA/ROCK1/pMLC signaling, which targets the expression and localization of Cx43 and is responsible for cell viability, apoptosis, and inflammation, resulting in retinal endothelial cell injury. ROCK inhibitors markedly ameliorate endothelial cell dysfunction, suggesting their therapeutic potential for diabetic retinopathy.
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http://dx.doi.org/10.1167/iovs.63.8.22DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9339693PMC
July 2022

An ultrasensitive and dual-recognition SERS biosensor based on [email protected] and aptamer functionalized [email protected] nanoparticles for detection of Staphylococcus aureus.

Talanta 2022 Jun 3;250:123648. Epub 2022 Jun 3.

The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.

An ultrasensitive and dual-recognition surface-enhanced Raman scattering (SERS) biosensor for Staphylococcus aureus (S. aureus) was constructed, which was based on teicoplanin (Tcp) functionalized gold-coated magnet nanoparticles ([email protected] NPs) as capture probe and S. aureus aptamer (Apt) functionalized silver coated gold nanoparticles ([email protected] NPs) as signal probe. Both Au NPs and [email protected] NPs were prepared by a green synthesis method. Especially, the synthesis method of [email protected] NPs reduced by chitosan (CS) was first reported in this work. Due to the great SERS enhancement based on the hot spot effect between Au NPs and [email protected] NPs, and the dual-recognition ability based on Tcp and Apt, the SERS biosensor was ultrasensitive and specific. A detection limit of 1.09 CFU mL with a broad dynamic linear (7.6 × 10-7.6 × 10 CFU mL) was achieved within 50 min without interference by other bacteria. Moreover, the SERS biosensor could be applied for detection of S. aureus in milk and orange juice samples. This study provides a green, rapid and ultrasensitive method to detect S. aureus, and also explores the high utilization value of CS and Tcp, which has a broad application prospect in detection of pathogenic bacteria.
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http://dx.doi.org/10.1016/j.talanta.2022.123648DOI Listing
June 2022

Insights into male androgenetic alopecia using comparative transcriptome profiling: HIF-1 and Wnt/β-catenin signaling pathways.

Br J Dermatol 2022 Jul 21. Epub 2022 Jul 21.

Department of Dermatology, Huashan Hospital, Fudan University, Shanghai, China.

Background: The key pathophysiological changes in androgenetic alopecia (AGA) are limited to hair follicles (HFs) in frontal and vertex regions, except for the occipital region.

Objective: To identify biological differences among HF subpopulations.

Methods: Paired vertex and occipital HFs from 10 male AGA donors were collected for RNA-seq assay. Furthermore, hair follicle and cell experiments were conducted on the identified key genes to reveal their roles in AGA.

Results: Transcriptome profiles revealed that 506 mRNAs, 55 miRNAs, and 127 lncRNAs were differentially expressed (DE) in the AGA vertex HFs. Furthermore, pathway analysis of mRNAs and microRNAs revealed the involvement of the HIF-1, Wnt/β-catenin, and focal adhesion pathways. Differential expression of HIF-1 prolyl hydroxylase enzymes (EGLN1, EGLN3) and Wnt/β-catenin pathway inhibitors (PEDF, SFRP2) were experimentally validated. In vitro studies revealed that EGLN1/EGLN3/PEDF/SFRP2 reduction stimulated dermal papilla cell (DPC) proliferation. Ex vivo HF studies showed that EGLN1/EGLN3/PEDF downregulation promoted HF growth, postponed HF catagen transition, and prolonged the anagen stage, suggesting that these genes may be potentially utilized as therapeutic targets for AGA.

Conclusion: We characterized key transcriptome changes in male AGA HFs, and found that HIF-1 pathway-related genes (EGLN1/EGLN3) and Wnt pathway inhibitors (PEDF, SFRP2) may play important roles in AGA.
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http://dx.doi.org/10.1111/bjd.21783DOI Listing
July 2022

Insight into biofilm-forming patterns: biofilm-forming conditions and dynamic changes in extracellular polymer substances.

Environ Sci Pollut Res Int 2022 Jul 19. Epub 2022 Jul 19.

College of Resource and Environment, Northeast Agricultural University, Harbin, 150030, China.

The microbial biofilm adheres to the surface of the carrier, which protects the pollutant-degrading bacteria and resists harsh environments; thus, research on biofilm-forming patterns will help promote the application of biofilms in wastewater treatment. Herein, univariate analysis and response surface methodology (RSM) confirmed that glucose and mannose at 3-5 g/L promoted biofilm formation. Notably, the microplate method demonstrated that compared to trivalent cations, divalent cations could more greatly enhance the activity (especially magnesium) of the biofilm matrix, and the period of biofilm formation in the three strains was divided into the following stages: initial attachment (0-10 h), microcolony (10-24 h), maturation (24-48 h), and dispersion (36-72 h). During maturation, large amounts of extracellular polysaccharides (EPs) and extracellular DNA (eDNA) were distributed in the extracellular and intracellular spaces, respectively, as observed by super-resolution structured illumination microscopy (SR-SIM). This study enhances the understanding of the characteristics and patterns of biofilm formation and can facilitate the application of biofilms in wastewater treatment.
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http://dx.doi.org/10.1007/s11356-022-21645-5DOI Listing
July 2022

Prognostic impact of high-risk factors and mutation in patients with stage II deficient mismatch repair colon cancer: a retrospective cohort study.

Ann Transl Med 2022 Jun;10(12):702

Department of Medical Oncology, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.

Background: Deficient mismatch repair (dMMR) is associated with a good prognosis in patients with stage II colon cancer and observation is recommended after surgery in these patients. In contrast, patients with high-risk factors and Kirsten rat sarcoma viral oncogene homolog () mutation is associated with a poor prognosis in colon cancer. However, the prognosis and treatment of patients with dMMR colon cancer combined with high-risk factors or mutation remains unclear. This study aimed to evaluate whether patients with dMMR colon cancer combined with high-risk factors or mutation require further treatment.

Methods: This single-center retrospective study included patients who received radical surgical resection and mismatch repair (MMR) immunohistochemical detection at The Sixth Affiliated Hospital of Sun Yat-sen University between May 2011 and March 2021. The high-risk factors and mutation were assessed by clinicopathological data and targeted sequencing. Associations with disease-free survival (DFS) were evaluated using multivariable Cox models.

Results: Among the 1,357 patients with stage II colorectal cancer included, 226 of these patients had dMMR. Patients in the dMMR group were more likely to be younger [<50 years: odds ratio (OR) =0.401, 95% CI: 0.288-0.558, P<0.001], with poor differentiation (OR =5.800, 95% CI: 3.437-9.787, P<0.001), no perineural invasion (OR =0.132, 95% CI: 0.047-0.368, P<0.001), and more than 12 excised lymph nodes (OR =0.427, 95% CI: 0.188-0.968, P=0.042). The disease-free survival (DFS) of patients with stage II dMMR colon cancer with high-risk factors was similar to that of patients without high-risk factors (hazard ratio (HR) =1.285, 95% CI: 0.273-6.051, P=0.607). A total of 836 patients had complete data regarding status. Compared with wild-type patients, patients with gene mutation had a trend of poor prognosis in patients with stage II colon cancer (HR=1.483, 95% CI: 0.983-2.239, P=0.061). In addition, dMMR appeared to be a protective factor in patients with mutation (HR =0.138, 95% CI: 0.019-1.002, P=0.0501).

Conclusions: The survival of patients with stage II dMMR colon cancer with high-risk factors was similar to that of patients without high-risk factors, regardless of the presence of mutation.
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http://dx.doi.org/10.21037/atm-22-2803DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9279762PMC
June 2022

Genomic instability genes in lung and colon adenocarcinoma indicate organ specificity of transcriptomic impact on Copy Number Alterations.

Sci Rep 2022 Jul 11;12(1):11739. Epub 2022 Jul 11.

Department of Medicine, Hematology/Oncology Section, Center for Cancer Prevention and Drug Development, University of Oklahoma Health Sciences Center (OUHSC), Oklahoma City, OK, USA.

Genomic instability (GI) in cancer facilitates cancer evolution and is an exploitable target for therapy purposes. However, specific genes involved in cancer GI remain elusive. Causal genes for GI via expressions have not been comprehensively identified in colorectal cancers (CRCs). To fill the gap in knowledge, we developed a data mining strategy (Gene Expression to Copy Number Alterations; "GE-CNA"). Here we applied the GE-CNA approach to 592 TCGA CRC datasets, and identified 500 genes whose expression levels associate with CNA. Among these, 18 were survival-critical (i.e., expression levels correlate with significant differences in patients' survival). Comparison with previous results indicated striking differences between lung adenocarcinoma and CRC: (a) less involvement of overexpression of mitotic genes in generating genomic instability in the colon and (b) the presence of CNA-suppressing pathways, including immune-surveillance, was only partly similar to those in the lung. Following 13 genes (TIGD6, TMED6, APOBEC3D, EP400NL, B3GNT4, ZNF683, FOXD4, FOXD4L1, PKIB, DDB2, MT1G, CLCN3, CAPS) were evaluated as potential drug development targets (hazard ratio [> 1.3 or < 0.5]). Identification of specific CRC genomic instability genes enables researchers to develop GI targeting approach. The new results suggest that the "targeting genomic instability and/or aneuploidy" approach must be tailored for specific organs.
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http://dx.doi.org/10.1038/s41598-022-15692-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9273645PMC
July 2022

Strengthen Synergistic Effect of Soft Carbon and Hard Carbon Toward High-Performance Anode for K-Ion Battery.

ACS Appl Mater Interfaces 2022 Jul 6;14(28):31879-31888. Epub 2022 Jul 6.

Department of Chemical and Environmental Engineering, The University of Nottingham Ningbo China, Ningbo 315100, PR China.

Synergistic effect of soft carbon and hard carbon has been proven to be useful for obtaining excellent anode materials for potassium ion battery, which is determined by the mixing degree of precursors. Inspired by the formation of proteins in biology, peptide bonds are used to connect the precursors of the two sort of carbon to prepare soft-hard hybrid carbons with stronger synergistic effects. The hard carbon domain with nanometer size is so highly distributed in the soft carbon that the synergistic effect between two sorts of carbon is significantly enhanced. After the optimization, the diffusion coefficient of as-prepared hybrid carbon (CSHC-1200) is 10 times larger than that of corresponding carbon synthesized by physical method. Consequently, CSHC-1200 can maintain a specific capacity of 71.6 mAh g at a high current density of 1600 mA g. It is believed that this new preparation route may bring a new perspective to the development of soft and hard composite carbon material anodes with high power density and ultralong service life.
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http://dx.doi.org/10.1021/acsami.2c05633DOI Listing
July 2022

Cellulomonas triticagri sp. nov., isolated from the rhizosphere soil of wheat (Triticum aestivum L.).

Arch Microbiol 2022 Jul 2;204(7):449. Epub 2022 Jul 2.

Key Laboratory of Agricultural Microbiology of Heilongjiang Province, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, 150030, People's Republic of China.

A Gram-positive, motile, rod-shaped and lignin-degrading novel actinomycete, designated strain NEAU-YY56, was isolated from the rhizosphere soil of wheat (Triticum aestivum L.) collected from Zhumadian, Henan Province, Central China and characterized using a polyphasic approach. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain NEAU-YY56 belonged to the genus Cellulomonas and exhibited 16S rRNA gene sequence similarities of 98.7, 98.2 and 98.1% to Cellulomonas pakistanensis JCM 18755, Cellulomonas denverensis JCM 14733 and Cellulomonas hominis JCM 12133, respectively. The whole-cell sugars were glucose, rhamnose and ribose. The peptidoglycan of strain NEAU-YY56 contained ornithine and glutamic acid. The phospholipid profile was found to contain diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol mannoside and two unknown glycolipids. The major menaquinone was MK-9(H). The major fatty acids (> 5.0%) were identified as anteiso-C, C, C and anteiso-C. Meanwhile, DNA G+C content was 74.7%. The morphological and chemotaxonomic properties of strain NEAU-YY56 were also confirmed the affiliation of the isolate to the genus Cellulomonas. However, physiological and biochemical characteristics indicated that strain NEAU-YY56 can be clearly differentiated from its closest relatives. In addition, the ANI values and dDDH levels between strain NEAU-YY56 and related Cellulomonas species were lower than the accepted threshold value. Therefore, it is concluded that strain NEAU-YY56 represents a novel species of the genus Cellulomonas, for which the name Cellulomonas triticagri sp. nov. is proposed. The type strain is NEAU-YY56 (= DSM 106717 = JCM 32550).
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http://dx.doi.org/10.1007/s00203-022-03036-zDOI Listing
July 2022

Giant Osmotic Energy Conversion through Vertical-Aligned Ion-Permselective Nanochannels in Covalent Organic Framework Membranes.

J Am Chem Soc 2022 Jul 28;144(27):12400-12409. Epub 2022 Jun 28.

Division of Physical Science and Engineering, 4700 King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Kingdom of Saudi Arabia.

Nanofluidic membranes have been demonstrated as promising candidates for osmotic energy harvesting. However, it remains a long-standing challenge to fabricate high-efficiency ion-permselective membranes with well-defined channel architectures. Here, we demonstrate high-performance osmotic energy conversion membranes based on oriented two-dimensional covalent organic frameworks (COFs) with ultrashort vertically aligned nanofluidic channels that enabled efficient and selective ion transport. Experiments combined with molecular dynamics simulations revealed that exquisite control over channel orientation, charge polarity, and charge density contributed to high ion selectivity and permeability. When applied to osmotic energy conversion, a pair of 100 nm thick oppositely charged COF membranes achieved an ultrahigh output power density of 43.2 W m at a 50-fold salinity gradient and up to 228.9 W m for the Dead Sea and river water system. The achieved power density outperforms the state-of-the-art nanofluidic membranes, suggesting the great potential of oriented COF membranes in the fields of advanced membrane technology and energy conversion.
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http://dx.doi.org/10.1021/jacs.2c04223DOI Listing
July 2022

Insights into the Response of Perennial Ryegrass to Abiotic Stress: Underlying Survival Strategies and Adaptation Mechanisms.

Life (Basel) 2022 Jun 8;12(6). Epub 2022 Jun 8.

College of Grassland Agriculture, Northwest A&F University, Yangling District, Xianyang 712100, China.

Perennial ryegrass ( L.) is an important turfgrass and gramineous forage widely grown in temperate regions around the world. However, its perennial nature leads to the inevitable exposure of perennial ryegrass to various environmental stresses on a seasonal basis and from year to year. Like other plants, perennial ryegrass has evolved sophisticated mechanisms to make appropriate adjustments in growth and development in order to adapt to the stress environment at both the physiological and molecular levels. A thorough understanding of the mechanisms of perennial ryegrass response to abiotic stresses is crucial for obtaining superior stress-tolerant varieties through molecular breeding. Over the past decades, studies of perennial ryegrass at the molecular and genetic levels have revealed a lot of useful information to understand the mechanisms of perennial ryegrass adaptation to an adverse environment. Unfortunately, molecular mechanisms by which perennial ryegrass adapts to abiotic stresses have not been reviewed thus far. In this review, we summarize the recent works on the genetic and molecular mechanisms of perennial ryegrass response to the major abiotic stresses (i.e., drought, salinity, and extreme temperatures) and discuss new directions for future studies. Such knowledge will provide valuable information for molecular breeding in perennial ryegrass to improve stress resistance and promote the sustainability of agriculture and the environment.
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http://dx.doi.org/10.3390/life12060860DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9224976PMC
June 2022

Arabinogalactan Proteins: Focus on the Role in Cellulose Synthesis and Deposition during Plant Cell Wall Biogenesis.

Int J Mol Sci 2022 Jun 13;23(12). Epub 2022 Jun 13.

Laboratory of Cell & Molecular Biology, Institute of Vegetable Science, Zhejiang University, Hangzhou 310058, China.

Arabinogalactan proteins (AGPs) belong to a family of glycoproteins that are widely present in plants. AGPs are mostly composed of a protein backbone decorated with complex carbohydrate side chains and are usually anchored to the plasma membrane or secreted extracellularly. A trickle of compelling biochemical and genetic evidence has demonstrated that AGPs make exciting candidates for a multitude of vital activities related to plant growth and development. However, because of the diversity of AGPs, functional redundancy of AGP family members, and blunt-force research tools, the precise functions of AGPs and their mechanisms of action remain elusive. In this review, we put together the current knowledge about the characteristics, classification, and identification of AGPs and make a summary of the biological functions of AGPs in multiple phases of plant reproduction and developmental processes. In addition, we especially discuss deeply the potential mechanisms for AGP action in different biological processes via their impacts on cellulose synthesis and deposition based on previous studies. Particularly, five hypothetical models that may explain the AGP involvement in cellulose synthesis and deposition during plant cell wall biogenesis are proposed. AGPs open a new avenue for understanding cellulose synthesis and deposition in plants.
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http://dx.doi.org/10.3390/ijms23126578DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9223364PMC
June 2022

A Research Based on Online Medical Platform: The Influence of Strong and Weak Ties Information on Patients' Consultation Behavior.

Healthcare (Basel) 2022 May 24;10(6). Epub 2022 May 24.

School of Economics and Management, Tongji University, Shanghai 200092, China.

As an indispensable part of contemporary medical services, Internet-based medical platforms can provide patients with a full range of multi-disciplinary and multi-modal treatment services. Along with the emergence of many healthcare influencers and the increasing connection between online and offline consultations, the operation of individual physicians and their teams on Internet-based medical platforms has started to attract a lot of attention. The purpose of this paper is to, based on an Internet platform, study how the information on physicians' homepages influences patients' consultation behavior, so as to provide suggestions for the construction of physicians' personal websites. We distinguish variables into strong- and weak-ties types, dependent on whether deep social interactions between physicians and patients have happened. If there exist further social interactions, we define the variable as the "strong ties" type, otherwise, "weak ties". The patients' consultation behavior will be expressed as the volume of online consultation, i.e., the number of patients. We obtained the strong and weak ties information of each physician based on EWM (entropy weight method), so as to establish a regression model with explained variable, i.e., the number of patients, and three explanatory variables, i.e., the strong and weak ties information, and their interaction term. The estimation results verified our hypotheses and proved to be robust. It showed that both strong and weak ties information can positively influence patients' consultation behavior, and the influence of weak ties information is greater. Regarding the positive influence of strong and weak ties, we found a trade off effect between them. Based on the results, we finalize with some suggestions on how to improve a physician's online medical consultation volume.
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http://dx.doi.org/10.3390/healthcare10060977DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9222327PMC
May 2022

Conjugated microporous polymer membranes for light-gated ion transport.

Sci Adv 2022 Jun 17;8(24):eabo2929. Epub 2022 Jun 17.

Chemical Engineering Program, Division of Physical Science and Engineering, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.

Inspired by the light-gated ion channels in cell membranes that play important roles in many biological activities, herein, we developed an artificial light-gated ion channel membrane out of conjugated microporous polymers. Through bottom-up design of the monomer molecular structure and by the electropolymerization method, the membrane pore size and thickness were precisely controlled on the molecular level. The obtained membrane exhibited uniform pore size and highly sensitive light-switchable response. The photoisomerization of the polymer chain resulted in a reversible "on and off" light control over the pore size and subsequently led to light-gated ion transport across the membrane for a series of ions including hydrogen, potassium, sodium, lithium, calcium, magnesium, and aluminum ions.
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http://dx.doi.org/10.1126/sciadv.abo2929DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9205585PMC
June 2022

Oral administration of TRAIL-inducing small molecule ONC201/TIC10 prevents intestinal polyposis in the mouse model.

Am J Cancer Res 2022 15;12(5):2118-2131. Epub 2022 May 15.

Center for Cancer Prevention and Drug Development, Hem-Onc Section, Department of Medicine, Stephenson Cancer Center, University of Oklahoma Health Sciences Center Oklahoma City, OK, USA.

Colorectal cancer (CRC) incidence is rising globally. Hence, preventing this disease is a high priority. With this aim, we determined the CRC prevention potential of the TRAIL-inducing small molecule ONC201/TIC10 using a preclinical model representing high-risk familial adenomatous polyposis (FAP) patients, mice. Prior to the efficacy study, optimal and non-toxic doses of ONC201 were determined by testing five different doses of ONC201 (0-100 mg/kg body weight (BW); twice weekly by oral gavage) in C57BL/6J mice (=6/group) for 6 weeks. BW gain, organ weights and histopathology, blood profiling, and the plasma liver enzyme profile suggested no toxicities of ONC201 at doses up to 100 mg/kg BW. For efficacy determination, beginning at six weeks of age, groups of male and female mice (≥20) treated with colon carcinogen azoxymethane (AOM) (AOM- ) were administered ONC201 (0, 25, and 50 mg/kg BW) as above up to 20 weeks of age. At termination, efficacy was determined by comparing the incidence and multiplicity of intestinal tumors between vehicle- and drug-treated groups. ONC201 showed a strong suppressive effect against the development of both large and small intestinal tumors in male and female mice. mice treated with ONC201 (50 mg/kg BW) showed >50% less colonic tumor incidence (<0.0002) than controls. Colonic tumor multiplicity was also significantly reduced by 68% in male mice (0.44 ± 0.11 in treated vs. 1.4 ± 0.14 in controls; <0.0001) and by 75% in female mice (0.30 ± 0.10 in treated vs. 1.19 ± 0.19 in controls; <0.0003) with ONC201 treatment (50 mg/kg BW). Small intestinal polyps were reduced by 68% in male mice (11.40 ± 1.19 in treated vs. 36.08 ± 2.62 in controls; <0.0001) and female mice (9.65 ± 1.15 in treated vs. 29.24 ± 2.51 in controls; <0.0001). Molecular analysis of the tumors suggested an increase in TRAIL, DR5, cleaved caspases 3/7/8, Fas-associated death domain protein (FADD), and p21 (WAF1) in response to drug treatment. Serum analysis indicated a decrease in pro-inflammatory serum biomarkers, such as IL1β, IL6, TNFα, G-CSF, and GM-CSF, in the ONC201-treated mice compared with controls. Our data demonstrated excellent chemopreventive potential of orally administered ONC201 against intestinal tumorigenesis in the AOM- mouse model.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9185612PMC
May 2022

Diverse polyacetylenes from Atractylodes chinensis and their anti-osteoclastogenesis activity.

Fitoterapia 2022 Jun 8;161:105233. Epub 2022 Jun 8.

Research Center for Drug Discovery, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, People's Republic of China. Electronic address:

The phytochemical investigation on Atractylodes chinensis afforded 15 polyacetylenes 1-15 and one meroterpenoid 16. Of the 16 isolates, compounds 4 and 9 are new ones, and compounds 8 and 16 are first reported from nature. In addition, the relative configuration of 1 and the available NMR data of compounds 1, 8, and 16 were first provided. Their structures were elucidated by extensive analysis of MS, UV, IR, and NMR spectroscopic data. Besides, all isolated compounds were evaluated for their effects on RANKL-induced osteoclastogenesis in BMMs. Among them, polyacetylenes 12-14 showed potent inhibitory activity with IC values of 0.67 ± 0.05 μM, 0.72 ± 0.31 μM, and 2.40 ± 0.41 μM, respectively. The current work demonstrates the polyacetylenes are the main active constituents of A. chinensis against osteoclastogenesis.
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http://dx.doi.org/10.1016/j.fitote.2022.105233DOI Listing
June 2022

Preparation and Properties of Municipal Solid Waste Incineration Alkali-Activated Lightweight Materials through Spontaneous Bubbles.

Polymers (Basel) 2022 May 30;14(11). Epub 2022 May 30.

School of Civil Engineering and Construction, Anhui University of Science and Technology, Huainan 232001, China.

A self-foaming alkali-activated lightweight material was prepared by the pretreatment of municipal solid waste incineration bottom ash (BA). The low weight could be achieved without adding a foaming agent by using the low-density and self-foaming expansion characteristics of BA in combination with a strong alkali. The effects of BA, liquid sodium silicate (LSS), and calcium hydroxide (CH) on dry and wet densities, as well as water absorption, are discussed. The results show that increasing the BA content can significantly improve the foaming effect and reduce the dry and wet densities of specimens. However, it also leads to a sudden decrease in compressive strength and a significant increase in water absorption. LSS and CH can significantly improve the ability to seal bubbles by accelerating condensation, and they further reduce dry and wet densities without significantly improving water absorption. It is most effective at BA, LSS, and CH contents of 60, 20, and 2%, respectively.
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http://dx.doi.org/10.3390/polym14112222DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9182782PMC
May 2022

Codon optimization of chicken β Gallinacin-3 gene results in constitutive expression and enhanced antimicrobial activity in transgenic Medicago sativa L.

Gene 2022 Aug 6;835:146656. Epub 2022 Jun 6.

Institute of Crop Germplasm Resources (Institute of Biotechnology), Shandong Academy of Agricultural Sciences, Shandong Provincial Key Laboratory of Crop Genetic Improvement, Ecology and Physiology, Jinan 250100, China.

Gallinacin-3 (Gal-3) is a newly discovered epithelial beta-defensin that acts as cationic antimicrobial peptides, and plays an important role in chicken innate immunity. However, the gallinacin-3 precursor containeda lengthy C-terminal region, which often hindered itsexpression. After codon optimization of Gal-3 and construction of an expression vector, the transgenic plants of Medicago sativa were obtained. Transgenic plants were validated and expression of proteins was detected. The antimicrobial activity of chicken β Gal-3 was analyzed and effects of chicken β Gal-3 on the body weight and intestinal microflora of mice were described. Our results demonstrated that the codon optimized chicken Gal-3 was stably expressed in transgenic Medicago sativa using the pCAMBIA3301 expression vector under the control of protein phosphatase (Ppha) promoter. Five transgenic plants with the highest expression of chicken β Gal-3 were selected, and were evaluated for the in vitro antimicrobial activity against Escherichia coli, Staphylococcus aureus and Salmonella typhi. Our findings confirmed that the Minimum Inhibitory Concentration (MIC) of the three bacterial strains were 32, 16 and 128 μg/mL, respectively. In addition, the effect of chicken Gal-3 on the body weight of mice fed with transgenic plants showed no significant deviation compared with that of the control group. Similarly, no loss of intestinal microflora was evident in the experimental group compared with the control group. Together, our findings demonstrate an alternative method for the stable expression of chicken Gal-3 withsignificant antibacterial effects and potential probiotics uses. In addition, this study may also be useful in the development of resistant M. sativa plants against pathogenic bacteria in future studies.
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http://dx.doi.org/10.1016/j.gene.2022.146656DOI Listing
August 2022

High-throughput UAV-based phenotyping provides insights into the dynamic process and genetic basis of rapeseed waterlogging response in the field.

J Exp Bot 2022 May 31. Epub 2022 May 31.

Macro Agriculture Research Institute, College of Resource and Environment, Huazhong Agricultural University, Wuhan, China.

Waterlogging severely affects the growth, development, and yield of crops. Accurate high-throughput phenotyping is important for exploring the dynamic crop waterlogging response process in the field and the genetic basis of waterlogging tolerance. In this study, a multi-model remote sensing phenotyping platform based on an unmanned aerial vehicle (UAV) was used to assess the genetic response of rapeseed (Brassica napus) to waterlogging by measuring morphological traits and spectral indices in two years. The dynamic responses of the morphological and spectral traits indicated that the rapeseed waterlogging response was severe before the middle stage within 18 days after recovery, but it partly decreased subsequently. Genome-wide association studies identified 289 and 333 loci associated with waterlogging tolerance in two years. Next, 25 loci with at least nine associations with waterlogging-related traits were defined as highly reliable loci, and 13 loci were simultaneously identified by waterlogging tolerance coefficients of morphological traits, spectral indices, and common factors. Forty candidate genes were predicted in the regions of 13 overlapping loci. Our study provides insights into the understanding of dynamic process and genetic basis of rapeseed waterlogging response in the field by a high-throughput UAV phenotyping platform. The highly reliable loci identified in this study are valuable for breeding waterlogging-tolerant rapeseed cultivars.
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http://dx.doi.org/10.1093/jxb/erac242DOI Listing
May 2022

Image processing with a multi-level ultra-fast three dimensionally integrated perovskite nanowire array.

Nanoscale Horiz 2022 Jun 27;7(7):759-769. Epub 2022 Jun 27.

Department of Electronic & Computer Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, China.

Besides its ubiquitous applications in optoelectronics, halide-perovskites (HPs) have also carved a niche in the domain of resistive switching memories (Re-RAMs). However owing to the material and electrical instability challenges faced by HP thin-films, rarely perovskite Re-RAMs are used to experimentally demonstrate data processing which is a fundamental requirement for neuromorphic applications. Here, for the first time, lead-free, ultrahigh density HP nanowire (NW) array Re-RAM has been utilized to demonstrate image processing design of convolutional kernels. The devices exhibited superior switching characteristics including a high endurance of 5 × 10 cycles, an ultra-fast erasing and writing speed of 900 ps and 2 ns, respectively, and a retention time >5 × 10 s for the resistances. The work is bolstered by an in-depth mechanistic study and first-principles simulations which provide evidence of electrochemical metallization triggering the switching. Employing the robust multi-level switching behaviour, image processing functions of embossing, outlining and sharpening were successfully implemented.
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http://dx.doi.org/10.1039/d2nh00183gDOI Listing
June 2022

Trust-Based Research: Influencing Factors of Patients' Medical Choice Behavior in the Online Medical Community.

Healthcare (Basel) 2022 May 18;10(5). Epub 2022 May 18.

The School of Management, Hefei University of Technology, Hefei 230009, China.

The medical service is a special credit commodity, and trust plays a very important role in patients' online medical choice behavior. By collecting information about the doctors on China's leading online medical platform (Platform A), a regression analysis model was constructed, based on the credibility theory model, which has the following three dimensions: ability trust, benevolence trust, and integrity trust. The results showed that the medical title of the doctors, their department's reputation, the number of gifts given to them, and the number of patients who registered with them after diagnosis, among other factors, had a significant, positive impact on the behavior of choosing doctors. Among these considerations, the number of patients registered after diagnosis had the greatest impact on the behavior of choosing doctors. This factor is the result of each doctor's personal brand management, which reflects their comprehensive ability, reputation and integrity. Compared with previous studies, this paper creatively analyzed the important influence of departmental reputation and the number of patients registered after diagnosis on medical choice behavior and puts forward that a doctor can use the number of patients registered after diagnosis to manage their personal brand. Based on the results of this study, we will also put forward suggestions from the perspectives of patients, doctors and the online medical community.
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http://dx.doi.org/10.3390/healthcare10050938DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9140699PMC
May 2022

Coral and it's symbionts responses to the typical global marine pollutant BaP by 4D-Proteomics approach.

Environ Pollut 2022 Aug 24;307:119440. Epub 2022 May 24.

State Key Laboratory of South China Sea Marine Resource Utilisation, Hainan University, Haikou, 570228, China; School of Life Sciences, Hainan University, Haikou, 570228, China; One Health Institute, Hainan University, Haikou, Hainan, 570228, China. Electronic address:

The symbiosis of corals, zooxanthellae, and microbes is the foundation of the coral reef ecosystem. In addition to global warming, marine pollutants are another important factor causing the breakdown of coral symbiosis. Benzo(a)pyrene (BaP) is a globally widespread marine environmental pollutant that poses a severe threat to marine ecosystems. However, responses of coral symbionts to global marine pollutant stress remain unclear. In this study, we selected Acropora formosa as the target coral to explore its response to 50 μg L BaP stress using diaPASEF proteomics and 16s rRNA microbiome analysis. The results showed that: 1) the coral symbionts were sensitive to BaP stress; 2) the photosynthetic system of zooxanthellae was crucial for the balance of symbiotic relationships; 3) the destruction of the photosynthetic system induced a zooxanthellae hypoxic stress response; 4) corals adapted to BaP stress by promoting non-essential protein degradation and changing energy metabolism strategies; 5) symbiotic bacteria showed strong adaptability to BaP. This study not only fills the gap in understanding the response mechanism of coral symbionts under BaP stress, but also provides fundamental data for coral reef protection strategies.
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http://dx.doi.org/10.1016/j.envpol.2022.119440DOI Listing
August 2022
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