Publications by authors named "Yuting Wang"

427 Publications

Clinical Evidence and Potential Mechanisms of Complementary Treatment of Formula for the Management of Serum Lipids and Obesity.

Evid Based Complement Alternat Med 2022 9;2022:7714034. Epub 2022 May 9.

Department of Pathology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China.

Objective: This study aims to evaluate the clinical effects of formula (LGZG), a famous TCM formula, for the management of serum lipids and obesity and preliminarily elucidates the bioactive components and the potential mechanism.

Methods: Cluster analysis was adopted to investigate the TCM herbs and their frequency of occurrence for treating hyperlipidemia and obesity in an academic experience database of Chinese famous TCM doctors (http://www.gjmlzy.com:83). Then, relevant randomized controlled trials (RCTs) about LGZG supplementation in improving lipid levels and obesity were retrieved and analyzed. Lastly, the integration of network pharmacology, as well as greedy algorithms, which are theoretically well founded for the set cover in computer science, was exploited to identify the bioactive components of LGZG and to reveal potential mechanisms for attenuation or reversal of hyperlipidemia and obesity.

Results: Based on the cluster analysis of 104 cases in TCM academic experience database, four TCM herbs in LGZG showed high-use frequency for treating hyperlipidemia and obesity. Meta-analysis on 19 randomized controlled trials (RCTs) with 1716 participants indicated that LGZG supplementation significantly decreased the serum levels of total triglycerides, total cholesterol, low-density lipoprotein cholesterol, BMI, and body weight and increased high-density lipoprotein cholesterol, compared with clinical control groups. No serious adverse effect was detected in all studies. Twenty-one bioactive components of LGZG, mainly flavonoids (i.e., naringenin, kaempferol, and kumatakenin), saponins (i.e., hederagenin), and fatty acids (i.e., eicosenoic acid), had the potential benefits possibly by regulating multiple targets such as PTPN1, CYP19A1, and ESR2, as well as a few complex pathways including the TNF signaling pathway, PPAR signaling pathway, arachidonic acid metabolism, fat digestion, and absorption.

Conclusion: The present study has proved the clinical value of LGZG as a complementary treatment for attenuation or reversal of hyperlipidemia and obesity. More high-quality clinical and experimental studies in the future are demanded to verify its effects and the precise mechanism of action.
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http://dx.doi.org/10.1155/2022/7714034DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9110158PMC
May 2022

Cortisol modulates glucose metabolism and oxidative response after acute high temperature stress in Pacific oyster Crassostrea gigas.

Fish Shellfish Immunol 2022 May 10. Epub 2022 May 10.

Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, 116023, China; Functional Laboratory of Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266235, China; Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian, 116023, China; Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Dalian Ocean University, Dalian, 116023, China.

Cortisol is the main stress hormone that plays crucial roles in energy metabolism and immune response in vertebrates. In the present study, the homologues of 11β-hydroxysteroid dehydrogenase type 1 (designated Cg11β-HSD1) and 5α-reductase 1 (designated Cg5αR1), the key enzymes related to cortisol metabolism, were identified from Pacific oyster Crassostrea gigas. The Cg11β-HSD1 harbored a conserved SDR domain, and Cg5αR1 contained a Steroid_dh domain and three transmembrane domains. The mRNA transcripts of Cg11β-HSD1 and Cg5αR1 were constitutively expressed in all the examined tissues of oysters, with the highest expression level in haemocytes and labial palp, respectively. After acute high temperature stress (28 °C), the mRNA expression level of Cg11β-HSD1 in hepatopancreas significantly up-regulated at 6 h and 12 h, and that of Cg5αR1 significantly up-regulated at 6 h, compared with the Blank group (11 °C). The concentration of cortisol and glucose, as well as the activities of superoxide dismutase (SOD) and catalase (CAT) in hepatopancreas all significantly up-regulated after acute high temperature stress, while the glycogen concentration in adductor muscle decreased significantly at 6 h and 12 h. After the blockage of Cg11β-HSD1 with metyrapone (MET), the cortisol concentration and the activities of SOD and CAT significantly decreased after acute high temperature stress, the glucose concentration in hepatopancreas significantly increased at 24 h, and the glycogen concentration in adductor muscle significantly increased at 6 h. These results collectively suggested that cortisol played a crucial role in regulating glucose metabolism and oxidative response in oysters after acute high temperature stress.
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http://dx.doi.org/10.1016/j.fsi.2022.05.010DOI Listing
May 2022

Pregnancy, fertility concerns and fertility preservation procedures in a national study of French breast cancer survivors.

Reprod Biomed Online 2022 Jan 19. Epub 2022 Jan 19.

Department of Medical Oncology, Institut Curie, Paris, France, University Paris, Paris, France.

Research Question: What are the real-life oncofertility practices in young women diagnosed with breast cancer?

Design: The FEERIC (FErtility, prEgnancy, contRaceptIon after breast Cancer in France) study is a web-based cohort study launched with the French collaborative research platform Seintinelles. The current work is based on the enrolment self-administered questionnaire of 517 patients with prior breast cancer diagnosis, free from relapse and aged 18 to 43 years at inclusion (from 12 March 2018 to 27 June 2019).

Results: Median age at breast cancer diagnosis was 33.6 years and 424 patients (82.0%) received chemotherapy. Overall, 236 (45.6%) patients were offered specialized oncofertility counselling, 181 patients underwent at least one fertility preservation procedure (FPP); 125 (24.2%) underwent one or more FPP with material preservation (oocytes n = 108, 20.9%; embryos n = 31, 6.0%; ovarian cryopreservation n = 6, 1.2%) and 78 patients received gonadotrophin-releasing hormone agonists (15.1%). With a median follow-up of 26.9 months after the end of treatments, 133 pregnancies had occurred in 85 patients (16.4%), including 20 unplanned pregnancies (15.0%). Most of the pregnancies were natural conceptions (n = 113, 87.6%), while 16 (12.4%) required medical interventions. For the planned pregnancies, median time to the occurrence of an ongoing pregnancy was 3 months. Patients who had an unplanned pregnancy reported lower rates of information on the consequences of the treatments on fertility (P = 0.036) at diagnosis.

Conclusions: Most of the patients were not offered proper specialized oncofertility counselling at the time of breast cancer diagnosis. Naturally conceived pregnancies after breast cancer were much more frequent than pregnancies resulting from the use of cryopreserved gametes. Adequate contraceptive counselling seems as important as information about fertility and might prevent unplanned pregnancies.
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http://dx.doi.org/10.1016/j.rbmo.2021.12.019DOI Listing
January 2022

Exosomal PD-L1 predicts response with immunotherapy in NSCLC patients.

Clin Exp Immunol 2022 May 6. Epub 2022 May 6.

Shanghai Lung Cancer Center, Shanghai Chest Hospital, Shanghai Jiao Tong University, West Huaihai Road 241, Shanghai, 200030, China.

Immune Check-Point Inhibitors (ICIs) have shown remarkable promise in treating tumors, including non-small cell lung cancer (NSCLC). Nevertheless, the treatment response rate is low. Studies have found that high expression of exosomal PD-L1 is one of the reasons for the low treatment response. Therefore, this study focused on the relationship of the exosomal PD-L1 and clinical response of immunotherapy in NSCLC patients to evaluate whether it could be used as a biomarker to predict the efficacy of ICIs. In this study, clinical information and blood samples of 149 NSCLC patients receiving ICIs were collected. The expression level of exosomal PD-L1 was detected by enzyme linked immunosorbent assay method, and the relationship between exosomal PD-L1 and the efficacy of ICIs was explored. Overall, our study found that the expression level of exosomal PD-L1 lower at pre-treatment, or the max fold increasing change higher at 3-6 weeks had higher disease control rate and longer progression free survival. It revealed that exosomal PD-L1 was associated with the treatment response of patients using ICIs and provided a new tool for the evaluation of clinical efficacy of lung cancer immunotherapy.
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http://dx.doi.org/10.1093/cei/uxac045DOI Listing
May 2022

Metabolomics insights into the prenatal exposure effects of polybrominated diphenyl ethers on neonatal birth outcomes.

Sci Total Environ 2022 Apr 30;836:155601. Epub 2022 Apr 30.

CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, China; University of Chinese Academy of Sciences, Beijing 100049, China; Liaoning Province Key Laboratory of Metabolomics, Dalian, China. Electronic address:

Background: Effects of polybrominated diphenyl ethers (PBDEs) on neonatal birth outcomes vary across previous studies, and the related mechanism investigation remains poorly understood, especially at the metabolic level.

Objectives: To evaluate the associations between prenatal PBDEs exposure and neonatal birth outcomes including gestational age, neonatal weight, birth length, head circumference (HC), Apgar score at 1 min (Apgar1) and 5 min, and further reveal the underlying metabolic disorders in a population-based birth cohort study.

Methods: Gas chromatography-triple quadrupole tandem mass spectrometry (GC-MS/MS) based targeted method and GC-MS based untargeted method were respectively conducted to obtain PBDE levels and metabolic profiles of 200 placental tissue samples from a typical e-waste recycling area (Guiyu) and reference area (Haojiang) in China. Spearman correlation and regression analyses were applied to assess the associations between the placental PBDE levels and birth outcomes. Metabolome-wide association studies and the meet-in-the-middle approach were employed to explore disruptions linking PBDE exposures and the corresponding adverse birth outcomes.

Results: Eight out of 27 PBDE congeners were detected in placenta with more than 50% frequency in at least one district and significantly higher in Guiyu than those in Haojiang. The lower HC and Apgar1 had significant associations with PBDE exposures after adjustment for potential confounders. A total of 66, 16 and 14 metabolites were significantly correlated with PBDE exposures, HC and Apgar1, respectively. 4 and 12 PBDE-related metabolites were significantly associated with the risks of decreasing neonatal HC and Apgar1. The disrupted metabolites were mainly involved in the pentose phosphate pathway, ascorbate metabolism, threonine metabolism, butanoate metabolism, lipid metabolism, and arginine biosynthesis.

Conclusions: In this birth cohort, higher placental PBDE levels were significantly associated with the lower HC and Apgar1. The associations might be modified by multiple metabolic disturbances through increasing oxidative stress, mediating neurotoxicity, maternal gut microbiota dysbiosis and vasodilatation regulation.
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http://dx.doi.org/10.1016/j.scitotenv.2022.155601DOI Listing
April 2022

Construction and Evaluation of Multiple Radiomics Models for Identifying the Instability of Intracranial Aneurysms Based on CTA.

Front Neurol 2022 11;13:876238. Epub 2022 Apr 11.

Department of Radiology, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China.

Background And Aims: Identifying unruptured intracranial aneurysm instability is crucial for therapeutic decision-making. This study aims to evaluate the role of Radiomics and traditional morphological features in identifying aneurysm instability by constructing and comparing multiple models.

Materials And Methods: A total of 227 patients with 254 intracranial aneurysms evaluated by CTA were included. Aneurysms were divided into unstable and stable groups using comprehensive criteria: the unstable group was defined as aneurysms with near-term rupture, growth during follow-up, or caused compressive symptoms; those without the aforementioned conditions were grouped as stable aneurysms. Aneurysms were randomly divided into training and test sets at a 1:1 ratio. Radiomics and traditional morphological features (maximum diameter, irregular shape, aspect ratio, size ratio, location, etc.) were extracted. Three basic models and two integrated models were constructed after corresponding statistical analysis. Model A used traditional morphological parameters. Model B used Radiomics features. Model C used the Radiomics features related to aneurysm morphology. Furthermore, integrated models of traditional and Radiomics features were built (model A+B, model A+C). The area under curves (AUC) of each model was calculated and compared.

Results: There were 31 (13.7%) patients harboring 36 (14.2%) unstable aneurysms, 15 of which ruptured post-imaging, 16 with growth on serial imaging, and 5 with compressive symptoms, respectively. Four traditional morphological features, six Radiomics features, and three Radiomics-derived morphological features were identified. The classification of aneurysm stability was as follows: the AUC of the training set and test set in models A, B, and C are 0.888 (95% CI 0.808-0.967) and 0.818 (95% CI 0.705-0.932), 0.865 (95% CI 0.777-0.952) and 0.739 (95% CI 0.636-0.841), 0.605(95% CI 0.470-0.740) and 0.552 (95% CI 0.401-0.703), respectively. The AUC of integrated Model A+B was numerically slightly higher than any single model, whereas Model A+C was not.

Conclusions: A radiomics and traditional morphology integrated model seems to be an effective tool for identifying intracranial aneurysm instability, whereas the use of Radiomics-derived morphological features alone is not recommended. Radiomics-based models were not superior to the traditional morphological features model.
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http://dx.doi.org/10.3389/fneur.2022.876238DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9037633PMC
April 2022

Sulfate-Enabled Nitrate Synthesis from Nitrogen Electrooxidation on a Rhodium Electrocatalyst.

Angew Chem Int Ed Engl 2022 Apr 20:e202204541. Epub 2022 Apr 20.

Institute of Molecular Plus, Department of Chemistry, School of Science, Tianjin University, Tianjin, 300072, China.

The electrocatalytic nitrogen oxidation reaction (NOR) to generate nitrate is gaining increasing attention as an alternative approach to the conventional industrial manufacture. But, current progress in NOR is limited by the difficulties in activation and conversion of the strong N≡N bond (941 kJ mol ). Herein, we designed to utilize sulfate to enhance NOR performance over an Rh electrocatalyst. After the addition of sulfate, the inert Rh nanoparticles exhibited superior NOR performance with a nitrate yield of 168.0 μmol g  h . The N isotope-labeling experiment confirmed the produced nitrate from nitrogen electrooxidation. A series of electrochemical in situ characterizations and theoretical calculation unveiled that sulfate promoted nitrogen adsorption and decreased the reaction energy barrier, and in situ formed sulfate radicals reduced the activation energy of the potential-determining step, thus accelerating NOR.
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http://dx.doi.org/10.1002/anie.202204541DOI Listing
April 2022

Effects of Serving as a State Functionary on Self-Rated Health: Empirical Evidence From China.

Front Public Health 2022 1;10:757036. Epub 2022 Apr 1.

School of Philosophy, Zhongnan University of Economics and Law, Wuhan, China.

Purpose: There is a strong link between occupation and self-rated health. Existing research has revealed the effects of occupation on self-rated health outcomes and the corresponding mechanisms. However, there is a lack of research on the effects of state services on self-rated health in China. Therefore, this study focuses on exploring the effects of serving as a state functionary in China on self-rated health to enrich research in related fields.

Method: Based on the data of 14,138 individuals collected from the 2016 China Labour-Force Dynamics Survey, the logit model was used to investigate the effects of serving as a state functionary on self-rated health and the difference in the effects across different populations.

Results: The results show that (1) serving as a state functionary has a significant positive effect on self-rated health; (2) self-rated health of elderly state functionaries is higher than that of younger state functionaries; (3) self-rated health of state functionaries in non-eastern regions is higher than that of state functionaries in eastern regions; and (4) state functionaries with lower education have higher self-rated health than highly-educated state functionaries; (5) Higher self-rated health of state functionaries is achieved primarily through better work time, better work environment and lower relative deprivation.

Conclusion: Serving as a state functionary in China has a significant positive correlation with self-rated health, with differences across populations of state functionaries. This study expands the current literature on the effects of occupation on self-rated health in the context of China.
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http://dx.doi.org/10.3389/fpubh.2022.757036DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9012441PMC
April 2022

Study of Sex Differences in Unmedicated Patients With Major Depressive Disorder by Using Resting State Brain Functional Magnetic Resonance Imaging.

Front Neurosci 2022 31;16:814410. Epub 2022 Mar 31.

Huaxi MR Research Center (HMRRC), Department of Radiology, West China Hospital of Sichuan University, Chengdu, China.

Some important clinical characteristics of major depressive disorder (MDD) differ between sexes. We explored abnormal spontaneous neuronal activity in MDD patients using the amplitude of low-frequency fluctuation (ALFF) and its relationship to clinical manifestations in male and female patients, to seek the neural mechanisms underlying sex-related differences in depression. Twenty-five male MDD patients, 36 female MDD patients, and 25 male and 36 female matched healthy controls (HC) were included. The ALFF difference was investigated among four groups, and partial correlation analysis was used to explore a possible clinical relevance. The main effect results of sex difference were located in the bilateral caudate nucleus and posterior cingulate gyrus. comparisons found that the male MDD patients showed decreased ALFF in the bilateral caudate nucleus and posterior cingulate gyrus when compared with female MDD patients/female HCs, and female MDD patients showed increased ALFF in the bilateral caudate nucleus and posterior cingulate gyrus when compared with male HCs. The average ALFF of the right caudate nucleus was positively correlated with illness duration in female MDD patients. Our results suggest that the sex-specific abnormal brain activity might be a potential pathomechanism of different symptoms in male and female MDD patients.
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http://dx.doi.org/10.3389/fnins.2022.814410DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008299PMC
March 2022

Corrosion behavior and mechanism of X80 steel in silty soil under the combined effect of salt and temperature.

RSC Adv 2021 Dec 20;12(1):129-147. Epub 2021 Dec 20.

College of Civil Engineering, Taiyuan University of Technology Taiyuan 030024 P. R. China

In this study, X80 pipeline steel was embedded in silty soil with different salinities and subjected to corrosion at a constant temperature for 24 h before electrochemical testing. The effect of soil medium, temperature, and salt content on the kinetics of corrosion behavior of X80 steel was analyzed. Furthermore, the compositions and structures of the corrosion products were analyzed by X-ray photoelectron spectroscopy and scanning electron microscopy. Based on the results, the anodic dissolution reaction mechanism of X80 steel in silty soil was determined, the differences in the corrosion process caused by different soil systems were comprehensively contrasted, and the impact of the migration process of heterogeneous silty soil on corrosion behavior under different conditions was systematically explored. Comparative analysis revealed that chloride ions possess strong adsorption ability at temperatures above freezing point and that more oxidized substances are present in the deposited layer on the surface of corroded steel, which facilitates the occurrence of corrosion under deposition. At temperatures below freezing point, the sulfate ions present in the pore solution contribute to crystallization-induced expansion and lead to swelling and deformation of the soil, rendering the X80 steel more prone to corrosion in sulfate corrosion environments.
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http://dx.doi.org/10.1039/d1ra08249cDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8978660PMC
December 2021

Risk factors and prognosis for esophageal fistula in patients with esophageal squamous cell carcinoma during radiotherapy.

Esophagus 2022 Apr 13. Epub 2022 Apr 13.

State Key Laboratory of Oncology in South China, Collaborative Innovation Centre for Cancer Medicine, Guangdong Esophageal Cancer Institute, Guangzhou, China.

Purpose: To determine risk factors, treatment outcomes, and prognostic factors for esophageal fistula (EF) in patients with esophageal squamous cell carcinoma (ESCC) during radiotherapy.

Methods: Between 2010 and 2018, 109 patients with EF during radiotherapy were retrospectively collected. A controlled cohort including 416 patients who received definitive chemoradiotherapy without EF was used to compare risk factors and survival outcomes. Univariate and multivariate logistic regression analyses were performed to identify predictors of EF. Propensity score matching (PSM) was applied to adjust for potential confounding factors.

Results: Multivariate analysis demonstrated that sex, body mass index, alcohol history, esophageal ulceration, primary tumor length, T stage, and absolute lymphocyte count were independent risk factors for EF. After PSM, patients with EF showed remarkably worse prognosis than those without EF (median overall survival: 13.0 versus 20.5 months; P = 0.009). For patients with EF, serum albumin level (≥ 35 g/L), subsequent radiotherapy, and fistula closure were associated with significantly prolonged survival. In addition, esophageal-mediastinum fistula and subsequent radiotherapy were positive predictors for fistula closure.

Conclusions: We identified risk factors for radiotherapy-related EF and its unfavorable prognosis in patients with ESCC. Of them, patients with serum albumin level of ≥ 35 g/L, subsequent radiotherapy after EF, and fistula closure had a more favorable survival.
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http://dx.doi.org/10.1007/s10388-022-00919-4DOI Listing
April 2022

Identification of Immune-Related Gene Signature and Prediction of CeRNA Network in Active Ulcerative Colitis.

Front Immunol 2022 22;13:855645. Epub 2022 Mar 22.

Institutes of Biology and Medical Sciences, Soochow University, Suzhou, China.

Background: Ulcerative colitis (UC) is an inflammatory disease of the intestinal mucosa, and its incidence is steadily increasing worldwide. Intestinal immune dysfunction has been identified as a central event in UC pathogenesis. However, the underlying mechanisms that regulate dysfunctional immune cells and inflammatory phenotype remain to be fully elucidated.

Methods: Transcriptome profiling of intestinal mucosa biopsies were downloaded from the GEO database. Robust Rank Aggregation (RRA) analysis was performed to identify statistically changed genes and differentially expressed genes (DEGs). Gene Set Enrichment Analysis (GSEA), Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG) were used to explore potential biological mechanisms. CIBERSORT was used to evaluate the proportion of 22 immune cells in biopsies. Weighted co-expression network analysis (WGCNA) was used to determine key module-related clinical traits. Protein-Protein Interaction (PPI) network and Cytoscape were performed to explore protein interaction network and screen hub genes. We used a validation cohort and colitis mouse model to validate hub genes. Several online websites were used to predict competing endogenous RNA (ceRNA) network.

Results: RRA integrated analysis revealed 1838 statistically changed genes from four training cohorts (adj. value < 0.05). GSEA showed that statistically changed genes were enriched in the innate immune system. CIBERSORT analysis uncovered an increase in activated dendritic cells (DCs) and M1 macrophages. The red module of WGCNA was considered the most critical module related to active UC. Based on the results of the PPI network and Cytoscape analyses, we identified six critical genes and transcription factor NF-κB. RT-PCR revealed that andrographolide (AGP) significantly inhibited the expression of hub genes. Finally, we identified XIST and three miRNAs (miR-9-5p, miR-129-5p, and miR-340-5p) as therapeutic targets.

Conclusions: Our integrated analysis identified four hub genes (, , , and ) regulated by NF-κB. We further revealed that AGP decreased the expression of hub genes by inhibiting NF-κB activation. Lastly, we predicted the involvement of ceRNA network in the regulation of NF-κB expression. Collectively, our results provide valuable information in understanding the molecular mechanisms of active UC. Furthermore, we predict the use of AGP and small RNA combination for the treatment of UC.
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http://dx.doi.org/10.3389/fimmu.2022.855645DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8980722PMC
April 2022

Target-Binding Accelerated Response for Sensitive Detection of Basal HO in Tumor Cells and Tissues via a Dual-Functional Fluorescence Probe.

Anal Chem 2022 04 5;94(15):5962-5969. Epub 2022 Apr 5.

Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710119, Shaanxi, P. R. China.

Aberrant production of HO is involved in cancer. The levels of HO are significantly higher in tumor cells than in normal cells. It is important to develop fluorescent probes to image basal HO selectively in tumor cells. So far, a cancer cell-targeting probe to image basal HO has not been reported. Thus, we developed a fluorescent probe, , which contains benzil as a HO-recognition site and biotin as a target binding motif for the selective and sufficient detection of HO in tumor cells. enables a selective fluorescence turn-on response to HO. The binding of the probe with biotin receptors can greatly accelerate the fluorescence response to HO. As a result, can sufficiently image basal HO in biotin receptor-positive cancer cells and tumor tissues. Finally, was successfully applied to discriminate between cancerous and normal tissues.
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http://dx.doi.org/10.1021/acs.analchem.2c00400DOI Listing
April 2022

Safety and immunogenicity of inactivated SARS-CoV-2 vaccines in people living with HIV.

Emerg Microbes Infect 2022 Dec;11(1):1126-1134

Key Laboratory of Molecular Biology for Infectious Diseases (Ministry of Education), Institute for Viral Hepatitis, Department of Infectious Diseases, the Second Affiliated Hospital of Chongqing Medical University, Chongqing, People's Republic of China.

It is important to know the safety and efficacy of vaccination in immunocompromised people living with HIV (PLWH), but currently, there is limited data on the inactivated SARS-CoV-2 vaccines' safety and immune responses in PLWH. In this prospective observational study, 139 PLWH and 120 healthy controls were enrolled and monitored for 21-105 days after a two-dose vaccination. The safety, anti-receptor binding domain IgG (anti-RBD-IgG) and anti-spike-IgG responses, and RBD-specific memory B cell (MBC) responses were evaluated. The overall adverse events within seven days were reported in 12.9% (18/139) of PLWH and 13.3% (16/120) of healthy controls. No serious adverse events occurred in both groups. Overall, the seroprevalence of anti-RBD-IgG in PLWH was significantly decreased (87.1% vs. 99.2%; <0.001). The geometric mean end-point titer (GMT) of anti-RBD-IgG in PLWH was also reduced, especially in patients with CD4 counts <200 cells/µL, regardless of age, gender, or HIV viral load. GMTs of anti-RBD-IgG in both PLWH and healthy controls declined gradually over time. Similar results were also observed in the anti-spike-IgG response. The frequency of RBD-specific MBCs in PLWH decreased (<0.05), and then remained stable over time. Lastly, through multivariate analysis, we found the factors that predicted a less robust response to inactivated vaccines in PLWH were a low CD4 count and long time interval after vaccination. In conclusion, inactivated vaccines are well-tolerated in PLWH but with low immunogenicity. Therefore, SARS-CoV-2 vaccines and booster doses should be given priority in PLWH, especially in patients with low CD4 counts. ClinicalTrials.gov identifier: NCT05043129..
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http://dx.doi.org/10.1080/22221751.2022.2059401DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9037169PMC
December 2022

Mechanism of cell death pathways in status epilepticus and related therapeutic agents.

Biomed Pharmacother 2022 May 31;149:112875. Epub 2022 Mar 31.

Department of Pharmaceutical Toxicology, School of Pharmaceutical Science, China Medical University, Shenyang 110001, China. Electronic address:

The most severe form of epilepsy, status epilepticus (SE), causes brain damage and results in the development of recurring seizures. Currently, the management of SE remains a clinical challenge because patients do not respond adequately to conventional treatments. Evidence suggests that neural cell death worsens the occurrence and progression of SE. The main forms of cell death are apoptosis, necroptosis, pyroptosis, and ferroptosis. Herein, these mechanisms of neuronal death in relation to SE and the alleviation of SE by potential modulators that target neuronal death have been reviewed. An understanding of these pathways and their possible roles in SE may assist in the development of SE therapies and in the discovery of new agents.
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http://dx.doi.org/10.1016/j.biopha.2022.112875DOI Listing
May 2022

Selenite Reduction by sp. YS02: New Insights Revealed by Comparative Transcriptomics and Antibacterial Effectiveness of the Biogenic Se Nanoparticles.

Front Microbiol 2022 10;13:845321. Epub 2022 Mar 10.

Institute of Biomedical and Health Science, School of Life and Health Science, Anhui Science and Technology University, Fengyang, China.

Biotransformation of selenite by microorganisms is an effective detoxification (in cases of dissimilatory reduction, e.g., to Se) and assimilation process (when Se is assimilated by cells). However, the current knowledge of the molecular mechanism of selenite reduction remains limited. In this study, a selenite-resistant bacterium was isolated and identified as sp. YS02. Strain YS02 reduced 93.2% of 5.0 mM selenite to selenium nanoparticles (SeNPs) within 24 h, and the produced SeNPs were spherical and localized intracellularly or extracellularly, with an average dimension of 140 ± 43 nm. The morphology and composition of the isolated and purified SeNPs were characterized using dynamic light scattering (DLS), scanning electron microscopy (SEM) with energy-dispersive X-ray (EDX) spectrometry, and Fourier transform infrared (FTIR) spectroscopy. FTIR spectroscopy indicated the presence of proteins, polysaccharides, and lipids on the surface of the isolated SeNPs. Furthermore, the SeNPs showed excellent antimicrobial activity against several Gram-positive and Gram-negative pathogenic bacteria. Comparative transcriptome analysis was performed to elucidate the selenite reduction mechanism and biosynthesis of SeNPs. It is revealed that 197 genes were significantly upregulated, and 276 genes were significantly downregulated under selenite treatment. Gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses revealed that genes associated with ABC transporters, sulfur metabolism, pentose phosphate pathway (PPP), and pyruvate dehydrogenase were significantly enhanced, indicating selenite is reduced by sulfite reductase with PPP and pyruvate dehydrogenase supplying reducing equivalents and energy. This work suggests numerous genes are involved in the response to selenite stress, providing new insights into the molecular mechanisms of selenite bioreduction with the formation of SeNPs.
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http://dx.doi.org/10.3389/fmicb.2022.845321DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8960269PMC
March 2022

Prophylactic Therapies for Morbidity and Mortality After Aneurysmal Subarachnoid Hemorrhage: A Systematic Review and Network Meta-Analysis of Randomized Trials.

Stroke 2022 Mar 31:STROKEAHA121035699. Epub 2022 Mar 31.

Department of Neurosurgery, University of Illinois at Chicago (S.A.-H.).

Background: Aneurysmal subarachnoid hemorrhage (aSAH) is associated with high mortality and morbidity. We aimed to determine the relative benefits of pharmacological prophylactic treatments in patients with aneurysmal subarachnoid hemorrhage by performing a network meta-analysis of randomized trials.

Methods: We searched Medline, Web of Science, Embase, Scopus, ProQuest, and Cochrane Central to February 2020. Pairs of reviewers independently identified eligible trials, extracted data, and assessed the risk of bias. Eligible trials compared the prophylactic effects of any oral or intravenous medications or intracranial drug-eluting implants to one another or placebo or standard of care in adult hospitalized patients with confirmed aneurysmal subarachnoid hemorrhage. We used the GRADE (Grading of Recommendations Assessment, Development and Evaluation) approach to assess the certainty of the evidence.

Results: We included 53 trials enrolling 10 415 patients. Nimodipine likely reduces all-cause mortality compared to placebo (odds ratio [OR],0.73 [95% CI, 0.53-1.00]; moderate certainty; absolute risk reduction (ARR), -3.35%). Nimodipine (OR, 1.46 [95% CI, 1.07-1.99]; high certainty; absolute risk increase, 8.25%) and cilostazol (OR, 3.73 [95% CI, 1.14-12.18]; moderate certainty; absolute risk increase, 23.15%) were the most effective treatments in improving disability at the longest follow-up. Compared to placebo, clazosentan (10 mg/kg; OR, 0.39 [95% CI, 0.22-0.68]; high certainty; ARR, -16.65%), nicardipine (OR, 0.48 [95% CI, 0.24-0.94]; moderate certainty; ARR, -13.70%), fasudil (OR, 0.55 [95% CI, 0.31-0.98]; moderate certainty; ARR, -11.54%), and magnesium (OR, 0.66 [95% CI, 0.46-0.94]; high certainty; ARR, -8.37%) proved most effective in reducing the likelihood of delayed cerebral ischemia.

Conclusions: Nimodipine and cilostazol are likely the most effective treatments in preventing morbidity and mortality in patients with aneurysmal subarachnoid hemorrhage. Clazosentan, nicardipine, fasudil, and magnesium showed beneficial effects on delayed cerebral ischemia and vasospasm but they were not found to reduce mortality or disability. Future trials are warranted to elaborately investigate the prophylactic effects of medications that may improve mortality and long-term functional outcomes, such as cilostazol and clazosentan.

Registration: URL: https://www.crd.york.ac.uk/PROSPERO/; Unique identifier: CRD42019122183.
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http://dx.doi.org/10.1161/STROKEAHA.121.035699DOI Listing
March 2022

Exposure to Metal Mixtures in Association with Cardiovascular Risk Factors and Outcomes: A Scoping Review.

Toxics 2022 Mar 1;10(3). Epub 2022 Mar 1.

Department of Epidemiology, Geisel School of Medicine at Dartmouth, Lebanon, NH 03756, USA.

Since the National Institute of Environmental Health Sciences (NIEHS) declared conducting combined exposure research as a priority area, literature on chemical mixtures has grown dramatically. However, a systematic evaluation of the current literature investigating the impacts of metal mixtures on cardiovascular disease (CVD) risk factors and outcomes has thus far not been performed. This scoping review aims to summarize published epidemiology literature on the cardiotoxicity of exposure to multiple metals. We performed systematic searches of MEDLINE (PubMed), Scopus, and Web of Science to identify peer-reviewed studies employing statistical mixture analysis methods to evaluate the impact of metal mixtures on CVD risk factors and outcomes among nonoccupationally exposed populations. The search was limited to papers published on or after 1998, when the first dedicated funding for mixtures research was granted by NIEHS, through 1 October 2021. Twenty-nine original research studies were identified for review. A notable increase in relevant mixtures publications was observed starting in 2019. The majority of eligible studies were conducted in the United States ( = 10) and China ( = 9). Sample sizes ranged from 127 to 10,818. Many of the included studies were cross-sectional in design. Four primary focus areas included: (i) blood pressure and/or diagnosis of hypertension ( = 15), (ii) risk of preeclampsia ( = 3), (iii) dyslipidemia and/or serum lipid markers ( = 5), and (iv) CVD outcomes, including stroke incidence or coronary heart disease ( = 8). The most frequently investigated metals included cadmium, lead, arsenic, and cobalt, which were typically measured in blood ( = 15). The most commonly utilized multipollutant analysis approaches were Bayesian kernel machine regression (BKMR), weighted quantile sum regression (WQSR), and principal component analysis (PCA). To our knowledge, this is the first scoping review to assess exposure to metal mixtures in relation to CVD risk factors and outcomes. Recommendations for future studies evaluating the associations of exposure to metal mixtures with risk of CVDs and related risk factors include extending environmental mixtures epidemiologic studies to populations with wider metals exposure ranges, including other CVD risk factors or outcomes outside hypertension or dyslipidemia, using repeated measurement of metals to detect windows of susceptibility, and further examining the impacts of potential effect modifiers and confounding factors, such as fish and seafood intake.
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http://dx.doi.org/10.3390/toxics10030116DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8955637PMC
March 2022

Large-Area Covalent Organic Polymers Membrane via Sol-Gel Approach for Harvesting the Salinity Gradient Energy.

Small 2022 May 24;18(20):e2107600. Epub 2022 Mar 24.

Key Laboratory of Bio-Inspired Smart Interfacial Science and Technology of Ministry of Education, School of Chemistry, Beihang University, Beijing, 100191, P. R. China.

Many materials with nanofluidic channels are exploited to achieve salinity gradient energy conversion. However, most materials are fragile, difficult to process, or only prepared into a limited size, which greatly restricts their practical application in the future. Herein, a covalent organic polymers membrane with high mechanical property and stability is fabricated, which can keep integrity in harsh conditions for up to 1 month. In addition, by using the sol-gel approach, a large-area membrane with an area of 26 × 26 cm is expediently fabricated in lab conditions. When the membrane is applied to salinity gradient energy conversion, the maximum output power density is up to 6.21 W m . This work provides a simple method for the fabrication of large-area membrane for salinity gradient energy conversion in future real-world applications.
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http://dx.doi.org/10.1002/smll.202107600DOI Listing
May 2022

The effect of serving as a danwei leader before retirement on self-rated post-retirement health: empirical evidence from China.

BMC Public Health 2022 03 23;22(1):573. Epub 2022 Mar 23.

School of Public Administration, Zhongnan University of Economics and Law, No. 182, Nanhu Avenue, Donghu New Technology Development Zone, Wuhan, Hubei, China.

Background: Worker health is often influenced by their occupation type, and the accumulative effect of occupation has a significant impact on their health after retirement. Studies show that the type and level of occupation before retirement directly impact workers' self-rated post-retirement health. However, there is little research on the self-rated post-retirement health of danwei leaders in China. This study seeks to examine the self-rated health level of Chinese danwei leaders after retirement. Furthermore, the differences between their self-rated health level and that of retirees from other occupations are explored by examining the accumulative effect of occupation and the mechanism underlying these differences.

Methods: Based on 5,910 samples' data from the 2018 China Longitudinal Aging Social Survey, ordinary least squares, logit, and propensity score matching models are used to investigate the self-rated health level of Chinese danwei leaders after retirement, their differences with other occupations, and the corresponding mechanism.

Results: The results show that retired danwei leaders have higher self-rated health levels than retirees in other occupations. This was mainly found among female, non-eastern, married, not living with children, and highly educated respondents. The difference in self-rated health between retired danwei leaders and other retired groups was influenced by their post-retirement income and social status.

Conclusions: In China, retired danwei leaders rated their health higher than retirees from other occupations. Danwei leaders have high socioeconomic status due to their occupation. Compared with other groups, their advantages are significant and enjoyed for a long time. Additionally, most danwei leaders have high social influence even after retirement and their higher income and social status have a positive impact on their self-rated health compared with other employees. This study provides empirical evidence from China and extends current literature on the effects of occupational type and level on self-rated health after retirement.
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http://dx.doi.org/10.1186/s12889-022-12937-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8941785PMC
March 2022

Development and Validation of Prognostic Nomogram for Postpartum Hemorrhage After Vaginal Delivery: A Retrospective Cohort Study in China.

Front Med (Lausanne) 2022 7;9:804769. Epub 2022 Mar 7.

Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University, Shenyang, China.

Background: Postpartum hemorrhage (PPH) is a common complication following vaginal delivery and in severe cases can lead to maternal death. A straightforward predictive model is required to enable prenatal evaluations by obstetricians to prevent PPH complications.

Methods: Data of patients who delivered vaginally after 37 weeks of gestation were retrospectively collected from the medical database at Shengjing Hospital of China Medical University for the period 2016 to 2020. PPH was defined as blood loss of 500 mL or more within 24 h of delivery, and important independent prognostic factors were determined using univariate and multivariate logistic regression analyses to construct nomograms regarding PPH.

Results: A total of 24,833 patients who delivered vaginally were included in this study. The training cohort included 22,302 patients who delivered between 2016 and 2019 and the external validation cohort included 2,531 patients who delivered during 2020. Nomogram was created using data such as age, race, occupation, parity, gestational weeks, labor time, neonatal weight, analgesic delivery, gestational diabetes mellitus, premature rupture of membranes, anemia, hypertension, adenomyosis, and placental adhesion. The nomogram has good predictive power and clinical practicality through the analysis of the area under the curve and decision curve analysis. Internal verification was performed on the nomogram for PPH, demonstrating consistency between the nomogram's predicted probability and actual probability.

Conclusions: The developed and validatable nomogram is a good predictor of PPH in vaginal delivery and can be used in clinical practice to guide obstetricians to administer preventive therapies before delivery.
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http://dx.doi.org/10.3389/fmed.2022.804769DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8936128PMC
March 2022

Long term outcome of prevention of liver cancer by hepatitis B vaccine: Results from an RCT with 37 years.

Cancer Lett 2022 06 19;536:215652. Epub 2022 Mar 19.

State Key Lab of Molecular Oncology and Department of Immunology, National Cancer Center / National Clinical Research Center for Cancer / Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, China. Electronic address:

We aimed to evaluate the long-term efficacy of the hepatitis B vaccine in China. In an initial efficacy study, participants were collected from a cluster-randomized clinical trial conducted in 1983-90 in Qidong. All the participants in the vaccination group were vaccinated at birth, 1 and 6 months of age, and no intervention was implemented to the control group. In this 37-year extended follow-up study, the Poisson regression method was employed to derive rates per 10 person-years. The frailty Cox proportional hazard regression models obtained the hazard ratio (HR). Cumulative incidence/mortality rates were calculated and compared with log-rank tests. 41,136 in the vaccination and 41,730 in the control group were recorded. The incidence rate of liver cancer was significantly lower in the vaccinated group than in the control group [HR, 0.28; 95% confidence interval (CI) 0.11-0.70, P = 0.007]. The vaccine offers 72% (95% CI, 30-89) protection to prevent the occurrence of liver cancer. There is 70% (95% CI, 23-88) protective efficacy against liver cancer deaths and 64% (95% CI, 27-82) benefits in the prevention of deaths associated with liver diseases. Hepatitis B vaccine given at birth shows excellent protective effects in preventing the development of liver cancer and reducing mortality from liver cancer and liver diseases.
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http://dx.doi.org/10.1016/j.canlet.2022.215652DOI Listing
June 2022

Practical COVID-19 Prevention Training for Obstetrics and Gynecology Residents Based on the Conceive-Design-Implement-Operate Framework.

Front Public Health 2022 3;10:808084. Epub 2022 Mar 3.

Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University, Shenyang, China.

Background: The spread of COVID-19 poses a challenge for obstetrics and gynecology (O&G) residents. In order to improve the theoretical knowledge and practical skills of residents in epidemic prevention and control, reduce work pressure and improve professional skills, effective and sound training models are required to improve the protection of O&G residents from COVID-19.

Method: A total of 38 standardized training O&G residents working in Shengjing Hospital of China Medical University in March 2020 was selected. They were randomly divided into intervention and control groups. The control group underwent a protection theory exposition according to the traditional training method, while the intervention group adopted a conceive-design-implement-operate (CDIO) mode, arranged training courses in combination with the O&G specialty, and completed four modules of CDIO. After the training, the theoretical knowledge and practical operation were assessed, and the work stress and occupational identity scales were assessed. The assessment results and scores of the two groups of residents were analyzed.

Results: Compared with the scores of the residents in the control group, the theoretical and technical scores of the residents in the intervention group significantly improved ( < 0.05). In the evaluation of organizational management, workload, interpersonal relationship, and doctor-patient relationship pressure, the scores of the intervention group were lower than those of the control group, with a statistical difference ( < 0.05). For the intervention group, the job stress and professional identity evaluation scores were significantly higher than those of the control group ( < 0.05).

Conclusion: The CDIO model can effectively enhance the theoretical knowledge and practical skills of O&G residents in COVID-19 epidemic prevention protocols to reduce work pressure and improve professional identity. In addition, it provides new ideas, methods, and approaches for future clinical practice training.
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http://dx.doi.org/10.3389/fpubh.2022.808084DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8927650PMC
March 2022

Accessory Gene Regulator () Allelic Variants in Cognate Strain Display Similar Phenotypes.

Front Microbiol 2022 25;13:700894. Epub 2022 Feb 25.

College of Basic Medical Sciences, Army Medical University, Chongqing, China.

The accessory gene regulator () quorum-sensing system is an important global regulatory system of and contributes to its pathogenicity. The system is divided into four groups based on the amino acid polymorphisms of AgrB, AgrD, and AgrC. The activation is group-specific, resulting in variations in activity and pathogenicity among the four groups. Strains with divergent system always have different phenotypes. In the present report, we, respectively, exchanged the system of a certain with other three alleles and assessed the corresponding phenotypes of these congenic strains. Replacement of the system led to significant variations in hemolytic activity, protein expression, and virulence gene expression comparing with that of the parental strain. Interestingly, we found that the biological characteristics of these congenic strains in the same strain background were highly similar to each other, and the allele-dependent differences of the systems were weakened. These findings indicate that the allele-dependent predilections of are determined by some factors in addition to the polymorphisms of AgrB, AgrD, and AgrC. Future studies may reveal the novel mechanism to improve our understanding of the network.
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http://dx.doi.org/10.3389/fmicb.2022.700894DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8919982PMC
February 2022

Crosstalk between adipose tissue and the microbiota-gut-brain axis in metabolic diseases.

Int J Biol Sci 2022 7;18(4):1706-1723. Epub 2022 Feb 7.

Key Laboratory of Acupuncture and Medicine Research of Ministry of Education, Nanjing University of Chinese Medicine, Nanjing, China.

Recently, the microbiota-gut-brain axis (MGBA) has emerged as a target for therapeutic innovation. Impairment of dynamic relationships within the MGBA promotes the pathological features of metabolic diseases. However, experimental data on the MGBA has limited clinical application. This review summarizes recent studies and proposes that exploring the interaction among peripheral organs and the MGBA could verify the dominant role of the latter in the onset of metabolic diseases and promote the clinical application of research outcomes. We first emphasize the molecular basis of metabolic diseases caused by MGBA disorders, which manifests as bidirectional relationship. We also summarize related therapeutic strategies, along with limitations in their clinical application. Adipose tissue (AT) is dynamic during metabolic activities and might interact with components in the MGBA. Therefore, it is interesting to explore the interplay among the MGBA and different kinds of AT, including thermogenic adipose tissue and white adipose tissue (WAT). In addition, we also evaluate the functional specificity of adipose tissue derived mesenchymal stem cells (ADSCs) and the beige adipose tissue. Understanding the heterogeneity and molecular basis of the interaction between different kinds of AT and the MGBA could accelerate innovation in the diagnosis and therapy of metabolic diseases.
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http://dx.doi.org/10.7150/ijbs.68786DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8898354PMC
April 2022

Natural Antioxidants and Hydrocolloids as a Mitigation Strategy to Inhibit Advanced Glycation End Products (AGEs) and 5-Hydroxymethylfurfural (HMF) in Butter Cookies.

Foods 2022 Feb 23;11(5). Epub 2022 Feb 23.

State Key Laboratory of Food Science and Technology, China-Canada Joint Lab of Food Science and Technology (Nanchang), Nanchang University, 235 Nanjing East Road, Nanchang 330047, China.

Maillard reaction during food processing contributes to the formation of some unpleasant heat-induced toxicants including advanced glycation end products (AGEs) and 5-hydroxymethylfurfural (HMF). The current study prepared butter cookies fortified with two dietary natural antioxidants (catechins and curcumin) and two dietary hydrocolloids (pectin and chitosan), and investigated their effects on formation of free N-(carboxymethyl)lysine (CML)/N-(carboxyethyl)lysine (CEL), protein-bound CML/CEL and HMF and on the sensory qualities of butter cookies. Meanwhile, three typical α-dicarbonyl compounds were also determined to identify possible correlations between α-dicarbonyl intermediates and formation of these harmful heat-induced products in butter cookies. Experimental data showed that catechin exhibited the strongest inhibitory effects on formation of AGEs and HMF, but its addition would impair the color and taste of cookies. On the other hand, chitosan was not so effective in inhibiting AGEs and HMF as compared to catechin, but its addition could increase the sensory qualities of butter cookies.
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http://dx.doi.org/10.3390/foods11050657DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8909267PMC
February 2022

Perceived Stigma and Self-Efficacy of Patients With Inflammatory Bowel Disease-Related Stoma in China: A Cross-Sectional Study.

Front Med (Lausanne) 2022 16;9:813367. Epub 2022 Feb 16.

Center for Inflammatory Bowel Diseases, Department of Gastroenterology, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Background: Patients with inflammatory bowel disease (IBD)-related stoma face physical, psychological, and social adjustment challenges. However, knowledge about stigmatization and self-management, which is important for clinical care and patient education strategies, is lacking.

Objective: To evaluate the level of stigma and self-management ability of Chinese patients with IBD-related stoma using an online questionnaire.

Methods: Participants were recruited from two general hospitals in mainland China and the internet platforms of the China Crohn's and Colitis Foundation (CCCF). Participants completed a questionnaire, which gathered data on sociodemographic, clinical, and experience in online groups, self-efficacy scale, and social impact scale. The influencing factors of self-efficacy and perceived stigma were assessed by stepwise multivariate regression analyses.

Results: One hundred and seventy-six respondents were included. Most of the respondents (78/176, 44.32%) spent between 500 and 999 RMB ($77-153) on ostomy care accessories monthly. Three patients reported using homemade ostomy products. The average score on the self-efficacy scale was 75.79 ± 23.91, which reflected a moderate level of self-efficacy, and 69 (39.2%) respondents had low-level self-efficacy. The average social impact scale score was 62.76 ± 12.69, which reflected a moderate level of perceived stigma. Forty-three (24.43%) patients experienced severe levels of perceived stigma. Stepwise multivariate regression analysis revealed that self-efficacy was associated with educational level ( = 0.007), whereas stigma was associated with nursing privacy ( = 0.021) and acceptance by the closest person ( = 0.005). A total of 91% of respondents who participated in online peer support groups believed the groups were helpful for disease management.

Conclusions: Chinese patients with IBD-related stoma reported a moderate degree of perceived stigma; their level of self-efficacy was low to moderate. High educational level was associated with high self-efficacy. Notably, acceptance of the stoma by the closest person was an influencing factor protecting patients from perceived stigma. Interventions aimed at improving patient education, reducing economic burden, and strengthening social support should be considered to help improve the living conditions of patients with IBD-related stoma.
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http://dx.doi.org/10.3389/fmed.2022.813367DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8888524PMC
February 2022

Structurally Disordered RuO Nanosheets with Rich Oxygen Vacancies for Enhanced Nitrate Electroreduction to Ammonia.

Angew Chem Int Ed Engl 2022 May 11;61(19):e202202604. Epub 2022 Mar 11.

School of Science, Institute of Molecular Plus, Tianjin University, Tianjin, 300072, China.

Electrochemical reduction of nitrate pollutants to ammonia has emerged as an attractive alternative for ammonia synthesis. Currently, many strategies have been developed for enhancing nitrate reduction to ammonia (NRA) efficiency, but the influence of the degree of structural disorder is still unexplored. Here, carbon-supported RuO nanosheets with adjustable crystallinity are synthesized by a facile molten salt method. The as-synthesized amorphous RuO displays high ammonia Faradaic efficiency (97.46 %) and selectivity (96.42 %), greatly outperforming the crystalline counterparts. The disordered structure with abundant oxygen vacancies is revealed to modulate the d-band center and hydrogen affinity, thus lowering the energy of the potential-determining step (NH *→NH *).
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http://dx.doi.org/10.1002/anie.202202604DOI Listing
May 2022

Enhances Early-Stage Infection by Inhibiting the Inflammatory Response.

Front Cell Infect Microbiol 2021 10;11:748461. Epub 2022 Feb 10.

Jangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, China.

The internalin family proteins, which carry the leucine repeat region structural motif, play diverse roles in (Lm) infection and pathogenesis. Although Internalin F, encoded by , was identified more than 20 years ago, its role in the Lm anti-inflammatory response remains unknown. Lm serotype 4b isolates are associated with the majority of listeriosis outbreaks, but the function of in these strains is not fully understood. In this study, we aimed to elucidate the role of in modulating the inflammatory response and pathogenesis of the 4b strain Lm NTSN. Strikingly, although was highly expressed at the transcriptional level during infection of five non-phagocytic cell types, it was not involved in adherence or invasion. Conversely, inlF did contributed to Lm adhesion and invasion of macrophages, and dramatically suppressed the expression of pro-inflammatory cytokines interleukin (IL)-1β and tumor necrosis factor (TNF-α). Consistent with the results, during Lm infection mice, significantly inhibited the expression of IL-1β and IL-6 in the spleen, as well as IL-1β, IL-6, and TNF-α in the liver. More importantly, contributed to Lm colonization in the spleen, liver, and ileum during the early stage of mouse infection intragastric administration, inducing severe inflammatory injury and histopathologic changes in the late stage. To our knowledge, this is the first report to demonstrate that mediates the inhibition of the pro-inflammatory response and contributes to the colonization and survival of Lm during the early stage of infection in mice. Our research partly explains the high pathogenicity of serovar 4b strains and will lead to new insights into the pathogenesis and immune evasion of Lm.
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http://dx.doi.org/10.3389/fcimb.2021.748461DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8866704PMC
April 2022

The mechanism of iodine enrichment in groundwater from the North China Plain: insight from two inland and coastal aquifer sediment boreholes.

Environ Sci Pollut Res Int 2022 Feb 25. Epub 2022 Feb 25.

School of Environmental Studies and State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan, 430074, China.

As an element relevant to human health, iodine is highly worthy of researchers' attention, especially the mechanism of iodine migration and enrichment in groundwater systems. A total of 43 groundwater, 1 seawater, 107 sediment, and 111 pore water samples from two boreholes (toward to Bohai Sea: BT, HH) were collected along a groundwater flow path at the North China Plain to investigate hydro-geochemical processes controlling groundwater iodine. High iodine groundwater (> 100 μg/L) was characterized by Na-Cl type, with high TDS values (827-2,400 mg/L) and high Cl (110-705 mg/L) and Br (416-1,180 μg/L) concentrations, which may be related to marine influence. Borehole BT and HH had pore water I concentration ranges of 1.4-132 μg/L and 3.6-830 μg/L, with high level that occurred near to coastline and corresponded to ancient transgression events. The results of sequential extraction of borehole sediments indicate that the fractions of sediment inorganic iodine mainly consisted of exchangeable, carbonate, and Fe-oxides associated fractions. Fe-oxides associated iodine was the main occurrence state in borehole BT far from the coastline, but high exchangeable iodine fractions (up to 92% of total extracted iodine) were observed in a high salinity borehole HH located near Bohai Bay, corresponding to the occurrence of high iodine pore water and groundwater. The analysis of iodine species indicates that iodide with strong migration ability dominated high iodine groundwater, pore water, and exchangeable sediment iodine, reflecting the occurrence of adsorption/desorption processes of iodine in groundwater system. High iodine groundwater and pore water exhibited iodine enrichment relative to Cl and Br, which suggests that iodine adsorbed on sediment desorbed under suitable pH and high solution ionic strength and subsequently released to pore water and aquifers. Inverse geochemical modeling stressed that ion exchange plays an important role in iodine enrichment of groundwater system.
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http://dx.doi.org/10.1007/s11356-021-18078-xDOI Listing
February 2022
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