Publications by authors named "Yuting Wang"

296 Publications

Nitrate electroreduction: mechanism insight, characterization, performance evaluation, and challenges.

Chem Soc Rev 2021 May 10. Epub 2021 May 10.

Department of Chemistry, School of Science, Institute of Molecular Plus, Tianjin University, Tianjin 300072, China. and Tianjin Key Laboratory of Molecular Optoelectronic Science, Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin 300072, China.

Excessive nitrate ions in the environment break the natural nitrogen cycle and become a significant threat to human health. So far, many physical, chemical, and biological techniques have been developed for nitrate remediation, but most of them require high post-processing costs and rigorous treatment conditions. In contrast, nitrate electroreduction is promising because it utilizes green electrons as reductants under ambient conditions. The recognition and mastering of the nitrate reaction mechanism is the premise for the design and synthesis of efficient electrocatalysts for the selective reduction of nitrate. In this regard, this review aims to provide an insight into the electrocatalytic mechanism of nitrate reduction, especially combined with in situ electrochemical characterization and theoretical calculations over different kinds of materials. Moreover, the performance evaluation parameters and standard test methods for nitrate electroreduction are summarized to screen efficient materials. Finally, an outlook on the current challenges and promising opportunities in this research area is discussed. This review provides a guide for development of electrocatalysts for selective nitrate reduction with a fascinating performance and accelerates the development of sustainable nitrogen chemistry and engineering.
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http://dx.doi.org/10.1039/d1cs00116gDOI Listing
May 2021

Evaluating the Quality of Reports About Randomized Controlled Trials of Acupuncture for Low Back Pain.

J Pain Res 2021 21;14:1141-1151. Epub 2021 Apr 21.

Department of Rehabilitation, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510000, People's Republic of China.

Objective: This study aims to improve the reporting quality of randomized controlled trials (RCTs) by evaluating RCTs of acupuncture for low back pain (LBP) based on the CONSORT and STRICTA statements.

Methods: Literature from the Cochrane Library, Medline, Embase, Ovid, China National Knowledge Infrastructure (CNKI), WanFang database, and Chongqing Weipu (VIP) was systematically searched from 2010 to 2020. The general characteristics and the overall quality score (OQS) of the literature were evaluated by two investigators. The agreement between investigators was calculated using Cohen's kappa statistics.

Results: A total of 31 RCTs were extracted in the final analysis. Based on the CONSORT statement, the items "title and abstract", "background and objectives", "intervention", "outcomes", "statistical methods", "baseline data", "outcomes and estimation" and "interpretation" have a positive rate of greater than 80%. The items "implementation", "generalizability" and "protocol" have a positive rate of less than 30%. Based on the STRICTA statement, the items "style of acupuncture", "needle retention time", "number of treatment sessions", "frequency and duration of treatment" and "precise description of the control or comparator" have a positive rate of greater than 80%. The item "extent to which the treatment was varied" has a positive rate of less than 30%. The agreements among most items are determined to be moderate or good.

Conclusion: The reporting quality of RCTs of acupuncture for LBP is moderate. Researchers should rigidly follow the CONSORT and STRICTA statements to enhance the quality of their studies.
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http://dx.doi.org/10.2147/JPR.S308006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8071706PMC
April 2021

Shape related features of intracranial aneurysm are associated with rupture status in a large Chinese cohort.

J Neurointerv Surg 2021 Apr 21. Epub 2021 Apr 21.

Department of Radiology, University of Washington School of Medicine, Seattle, Washington, USA.

Background: To investigate the prevalence of small ruptured saccular intracranial aneurysms (sIA) in a Chinese cohort and to identify factors associated with rupture status of sIAs.

Methods: Consecutive patients with confirmed sIAs by DSA from January 2015 to July 2019 were included. Demographic and aneurysmal features, including maximal diameter, location, irregularity (lobulated or with blebs), and aspect ratio (AR, defined as height divided by neck width) were recorded and analyzed. Mixed effect logistic regression was used in multivariate analysis.

Results: We analyzed 1514 sIAs in a Chinese cohort of 1216 patients, including 651 ruptured and 863 unruptured sIAs. Median aneurysm size was 5.7 mm for ruptured aneurysms, with 66.1% <7 mm in maximal diameter, and 40.2% measuring <5 mm. The median PHASES score of ruptured sIAs was 5. In multivariate analysis, male sex, hypertension, locations other than the internal carotid artery, irregularity (lobulated or with blebs), and higher AR were independently associated with rupture status (OR for irregularity, 2.88, 95% CI 2.20 to 3.77, p<0.001; OR for AR, 1.12, 95% CI 1.01 to 1.24, p=0.036). However, maximal diameter was not significantly associated with rupture status (p=0.72).

Conclusions: In this cohort, ruptured sIAs were frequently smaller than 7 mm. Shape related features, such as irregularity and higher AR, were associated with the ruptured status of sIAs, irrespective of diameter. PHASES seems to be inadequate in sIA risk stratification. Shape related parameters may be further investigated in prospective studies.
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http://dx.doi.org/10.1136/neurintsurg-2021-017452DOI Listing
April 2021

Antifungal activity and potential mechanism of Asiatic acid alone and in combination with fluconazole against Candida albicans.

Biomed Pharmacother 2021 Apr 10;139:111568. Epub 2021 Apr 10.

Department of Clinical Pharmacy, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Shandong Engineering and Technology Research Center for Pediatric Drug Development, Shandong Medicine and Health Key Laboratory of Clinical Pharmacy, Jinan, Shandong Province 250014, People's Republic of China. Electronic address:

Candida albicans (C. albicans) infection remains a challenge to clinicians due to the limited available antifungals. With the widespread use of antifungals in the clinic, the drug resistance has been emerging continuously, especially fluconazole. Therefore, searching for new antifungals, active constituents of natural or traditional medicines, and approaches to overcome antifungals resistance is needed. This study investigated the activity of Asiatic acid (AA) alone and in combination with fluconazole (FLC) against C. albicans in vitro and in vivo. The in vitro studies indicated that the drug combination had a synergistic effect on FLC-resistant C. albicans, with fractional inhibitory concentration index (FICI) of 0.25. And when AA at the dose of 32 µg/mL, the drug combination group could decrease the sessile minimum inhibitory concentration (sMIC) of FLC from > 1024 µg/mL to 0.125-0.25 µg/mL within 8 h against C. albicans biofilms, even with the FICI > 0.5. In vivo, the antifungal efficacy of AA used alone and in combination with FLC was evaluated by Galleria mellonella (G. mellonella) larvae. The drug combination group prolonged the survival rate and reduced tissue invasion of larvae infected with resistant C. albicans. Furthermore, mechanism studies indicated that the antifungal effects of AA in combination with FLC might be associated with the inhibition of drug efflux pump, the accumulation of reactive oxygen species (ROS) and the inhibition of hyphal growth. These findings might provide novel insights for overcoming drug resistance of C. albicans and bring new reference data for the development and application of AA in future.
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http://dx.doi.org/10.1016/j.biopha.2021.111568DOI Listing
April 2021

Identification of intra-individual variation in intracranial arterial flow by MRI and the effect on computed hemodynamic descriptors.

MAGMA 2021 Apr 11. Epub 2021 Apr 11.

Department of Radiology and Biomedical Imaging, University of California San Francisco, San Francisco, USA.

Objectives: To determine the intra-individual flow variation in serially acquired studies, and the influence of this variation on subsequent hemodynamic simulations using the inlet flow as a boundary condition. Author: Kindly check and confirm whether the corresponding authors are correctly identified.Confirmed.

Materials And Methods: This prospective study included 51 patients (37 females and 14 males) with unruptured intracranial aneurysms who have received more than three times follow-up of 2D phase-contrast MR. The flow and velocity parameters were extracted to calculate the reproducibility and variation. Patient-specific computational fluid dynamics simulations were performed using the measured flows.

Results: Intraclass correlation coefficients for mean and maximum velocity and flow parameters ranged from 0.77 to 0.90. A 10% CV of mean flow was identified. Variations of 10% in inlet flow resulted in hemodynamic changes including 41.41% of peak systolic wall shear stress; 39.13% of end-diastolic wall shear stress; 2.79% of low shear area at peak systole; 2.12% of low shear area at end diastole: 47.57% of time-averaged wall shear stress; and 0.17% of oscillatory shear index.

Conclusion: This study identified 10% of intra-individual mean flow variation on phase-contrast MR. Intra-individual flow variation resulted in a non-negligible variation in wall shear stress, but relatively small variation in low shear area in hemodynamic calculations.
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http://dx.doi.org/10.1007/s10334-021-00917-0DOI Listing
April 2021

Serum lipid profiling analysis and potential marker discovery for ovarian cancer based on liquid chromatography-Mass spectrometry.

J Pharm Biomed Anal 2021 May 26;199:114048. Epub 2021 Mar 26.

CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.

Low early diagnosis rate and unclear pathogenesis are the primary reasons for the high mortality of epithelial ovarian cancer (EOC). Lipidomics is a powerful tool for marker discovery and mechanism explanation. Hence, a ultra high-performance liquid chromatography-mass spectrometry based non-targeted lipidomics analysis was performed to acquire lipid profiling of 153 serum samples including healthy control (HC, n = 50), benign ovarian tumor (BOT, n = 41), and EOC (n = 62) to reveal lipid disturbance, then differential lipids were verified in another sample set including 187 sera. Significant lipid disturbance occurred in BOT and EOC, fatty acid, lyso-phosphatidylcholine, and lyso-phosphatidylethanolamine were observed to be increased in BOT and EOC subjects, while phosphatidylcoline, ether phosphatidylcoline (PC-O), ether phosphatidylethanolamine (PE-O), and sphingomyelin significantly decreased. Compared with BOT, PC-Os and PE-Os presented a greater reduction in EOC, and serum ceramide increased only in EOC. Moreover, potential markers consisting of 4 lipids were defined and validated for EOC diagnosis. High areas under the curve (0.854∼0.865 and 0.903∼0.923 for distinguishing EOC and early EOC from non-cancer, respectively) as well as good specificity and sensitivity were obtained. This study not only revealed the characteristics of lipid metabolism in EOC, but also provided a potential marker pattern for aiding EOC diagnosis.
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http://dx.doi.org/10.1016/j.jpba.2021.114048DOI Listing
May 2021

Psychological distress and sleep disturbance throughout thyroid nodules screening, diagnosis and treatment.

J Clin Endocrinol Metab 2021 Apr 8. Epub 2021 Apr 8.

Health and Management Center, West China Hospital of Sichuan University, Chengdu, China.

Background: Many controversies exist regarding screening and treatment of thyroid cancer (TC), especially papillary thyroid microcarcinoma (PTMC). The aim of this study was to evaluate patients' psychological distress and sleep disturbance throughout thyroid nodules (TNs) screening, diagnosis and treatment.

Materials And Methods: A total of 2834 participants (1153 participants with TNs) were enrolled during the screening phase, and 1105 individuals with TNs (87 individuals with TC) were enrolled during the diagnosis phase. Of the 87 TC patients, 66 underwent immediate operation (OP), and 21 patients with PTMC opted for active surveillance (AS). Four validated scales were applied to quantify the outcome indicators at prescreening, postscreening, postdiagnosis and posttreatment.

Results: Higher psychological distress and sleep disturbance were found postscreening than prescreening in subjects with TNs, but no differences in those absence of nodules. Compared to postscreening, higher scores of psychological distress and sleep disturbance were identified in patients with suspicious TC treated with fine needle aspiration (FNA) or AS. Lower psychological distress and sleep disturbance were noted for benign nodule patients than for TC patients. OP for TC, especially PTMC, did not alleviate psychological distress or sleep disturbance compared with the same parameters in patients who underwent AS.

Conclusions: Based on the findings of impaired psychological health and sleep quality, screening for TNs in adults who show no symptoms should be performed with caution. Psychological distress and sleep disturbance should also be taken into consideration when FNA is performed for suspected TC or OP for PTC, especially PTMC.
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http://dx.doi.org/10.1210/clinem/dgab224DOI Listing
April 2021

Stereotypes of Social Groups in Mainland China in Terms of Warmth and Competence: Evidence from a Large Undergraduate Sample.

Int J Environ Res Public Health 2021 03 30;18(7). Epub 2021 Mar 30.

Lab of Light and Physiopsychological Health, National Center for International Research on Green Optoelectronics, South China Normal University, Guangzhou 510006, China.

The Stereotype Content Model (SCM) has been validated in multiple countries and regions. However, previous validation studies in China have been limited by small sample size. The current research increased the sample size (n = 184 in the pilot study; n = 1315 and n = 268 in the formal study) to validate the SCM in mainland China in study 1. Supporting the SCM, 41 social groups were clustered into four quadrants based on warmth and competence dimensions. 35 of the 41 target groups (85.37%) receive ambivalent stereotype. Perceived warmth and competence were positively correlated ( = 0.585, < 0.001). Status and competence were positively related ( = 0.81, < 0.001), and competition and warmth were negatively related ( = -0.77, < 0.001). In addition, 24 typical social groups were selected and a list of stereotype words for these groups was developed in study 2 (n = 48, n = 52). The implications of the emerging social groups and the applications of this stereotype word list are discussed.
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http://dx.doi.org/10.3390/ijerph18073559DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8037077PMC
March 2021

Preparation of Fe-MIL(100)-encapsulated magnetic g-CN for adsorption of PPCPs from aqueous solution.

Environ Sci Pollut Res Int 2021 Mar 24. Epub 2021 Mar 24.

Gansu Key Laboratory for Environmental Pollution Prediction and Control, College of Earth and Environmental Sciences, Lanzhou University, Lanzhou, 730000, People's Republic of China.

In the present work, the Fe-MIL(100) was encapsulated on the outer surface of magnetic g-CN through a simple method to synthesize a novel adsorbent. The as-prepared g-CN/MnFeO/Fe-MIL(100) was characterized with scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), BET specific surface area (BET), vibrating sample magnetometry (VSM), and plasma emission spectrometry (PES). The g-CN/MnFeO/Fe-MIL(100) possessed rough surface, large surface area (303.68 m/g), mesoporous structure and magnetic properties, which exhibited excellent adsorption performance for ciprofloxacin (CIP), oxytetracycline (OTC) and indomethacin (IDM) with the maximum adsorption capacities reaching up to 45.51, 64.34 and 103.91 mg/g, respectively. The adsorption processes of all three PPCPs could be described by different kinds of isotherms and kinetic models. Additionally, the adsorption capacity of the resulting adsorbent could maintain 73.43% of the first adsorption capacity even after ten cycles. Finally, the possible adsorption mechanisms of g-CN/MnFeO/Fe-MIL(100) for CIP/OTC/IDM were proposed. Thus, g-CN/MnFeO/Fe-MIL(100) possessed excellent features of high adsorption capacity, fast removal rate, easy synthesis, salt resistance and magnetic separation, which showed great potential application to be used as an effective adsorbent for adsorptive removal of PPCPs in wastewater.
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http://dx.doi.org/10.1007/s11356-021-13550-0DOI Listing
March 2021

Effects of packaging methods on the quality of heavy metals-free preserved duck eggs during storage.

Poult Sci 2021 May 16;100(5):101051. Epub 2021 Feb 16.

Engineering Research Center of Biomass Conversion, Ministry of Education, Nanchang University, Nanchang 330047, China; State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China. Electronic address:

Preserved eggs without adding heavy metals in the pickling solution (heavy metals-free preserved eggs) have been developed, but it was found that the undesirable phenomenon such as dry shrinkage and fading occurred when they were not packaged and stored at room temperature. In this study, the effects of 5 packaging methods on the quality of heavy metals-free preserved eggs during storage were systematically studied. These methods included storage at room temperature and 4°C without packaging, wrapping with plastic bags, paraffin coating, and vacuum package. Through adopting these 5 packaging methods, the results showed that the moisture content and pH of the albumen decreased continuously, the mass loss rate increased continuously, the content of total volatile basic nitrogen increased firstly and then decreased, and the albumen hardness increased continuously. No microorganisms were detected in all samples with the 5 packaging methods during storage. Among them, the uncoated preserved eggs suffered the most serious moisture loss and mass loss, and the pH dropped at the fastest rate, followed by the preserved eggs wrapped in plastic bags. Preserved eggs stored at low temperature tended to turn yellow during storage, and the albumen showed higher hardness. The packaging method of paraffin coating performed the best in preventing the moisture loss of the albumen and the weight loss, which only decreased by 0.34 and 1.24%, respectively, after 3 mo. The best springiness, the darkest color, and the highest sensory score were found in the vacuum-packed preserved eggs after 3 mo of storage. It was concluded that paraffin coating and vacuum packing had better effect, while plastic bag packing showed the worst preservation performance for heavy metals-free preserved eggs.
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http://dx.doi.org/10.1016/j.psj.2021.101051DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8020475PMC
May 2021

Neonatal hepatitis B vaccination protects mature adults from occult virus infection.

Hepatol Int 2021 Mar 22. Epub 2021 Mar 22.

State Key Lab of Molecular Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 17 Panjiayuan South Lane, Beijing, 100021, People's Republic of China.

Background: Among elder children/young adults who received hepatitis B virus (HBV) vaccination during infancy, the serological status of HBsAg-negative and anti-HBc-positive [HBsAg(-)/anti-HBc(+)] was frequently reported, indicating potential occult HBV infection (OBI). It is required to define the long-term protection of neonatal vaccination against OBI in their mature adulthood.

Methods: Building upon the 1983-1990 established Qidong Hepatitis B Intervention Study, we sampled 10% of the 28-35-year-old participants, who remained in the cohort by 2012. Each participant was tested for serological markers of HBsAg, anti-HBs, HBeAg, anti-HBe and anti-HBc. HBV-DNA and relaxed circular DNA (rcDNA) were determined in some HBsAg(-)/anti-HBc(+) individuals.

Results: Totally, 3615 individuals from the neonatal vaccination group and 3100 individuals from the control group donated blood samples, respectively. In the vaccination group, the prevalence of HBsAg was 1.58% (57/3615), HBsAg(-)/anti-HBc(+) was 4.70% (170/3615), significantly lower than in the control group, which was 7.45% (231/3100) and 19.48% (640/3100) respectively (all p < 0.001). With aging, HBsAg(-)/anti-HBc(+) prevalence increased in the sampled participants from the control group (p < 0.001), but uncertain from the vaccination group. Of HBsAg(-)/anti-HBc(+), HBV-DNA was detected in 13.08% (17/130) from the vaccination group, and in 4.18% (12/287) from the control group. HBV rcDNA was detected in most sera that were tested positive for HBV-DNA.

Conclusions: OBI occurred in some vaccinated adults. However, neonatal HBV vaccination kept the effective protection against OBI in mature adults.
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http://dx.doi.org/10.1007/s12072-021-10156-zDOI Listing
March 2021

Improvement in mild anti-IgLON5 encephalopathy without immunotherapy: a case report.

BMC Neurol 2021 Mar 17;21(1):120. Epub 2021 Mar 17.

Department of Neurology, Tangshan Gongren Hospital, 27 Wenhua Road, Tangshan, 063000, Hebei, People's Republic of China.

Background: Anti-IgLON5 antibody-related encephalopathy is a recently discovered and rare autoimmune disease, and its diagnosis and treatment are more challenging than for other autoimmune encephalopathic diseases. Sleep disorder is the most prominent symptom of the disease. It can also present with gait instability, dysarthria, dysphagia, dementia, ataxia, autonomic nervous system dysfunction, chorea, vertical gaze paralysis, and other symptoms. Immunotherapy remains the primary treatment for this disease; however, there is no definitive conclusion regarding the effect of immunotherapy. The clinical symptoms of the reported cases of anti-IgLON5 antibody-related encephalopathy were generally severe. However, the symptoms in our patient were mild and relieved without immunotherapy, unlike the previously reported cases.

Case Presentation: A 62-year-old man presented with behavioural abnormalities and involuntary movements after nearly 2 months of fever and headache. He also had symptoms of mild sleep disorder. Due to the abnormal levels of infection-related indicators, antiviral treatment was started on the day of admission. The serum analysis confirmed the presence of IgLON5 antibody, and the patient was found to be genetically susceptible. The patient's symptoms resolved rapidly without immunotherapy and did not recur.

Conclusions: This case demonstrated that IgLON5 antibody-related encephalopathy might have mild manifestations. Infection and a genetic predisposition may be important causes for the disease. Patients with a mild disease may have a better prognosis.
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http://dx.doi.org/10.1186/s12883-021-02145-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7968182PMC
March 2021

Mn/Mn co-doped LaMAlO (M = Mg, Zn) luminescent materials: electronic structure, energy transfer and optical thermometric properties.

Dalton Trans 2021 Apr 16;50(13):4651-4662. Epub 2021 Mar 16.

Key Laboratory of In-Fiber Integrated Optics, Ministry Education of China, and College of Physics and Opotoelectronic Engineering, Harbin Engineering University, Harbin 150001, China.

Dual-emitting manganese ion doped LaMAlO (M = Mg, Zn) phosphors were prepared by substituting Zn/Mg with Mn and replacing Al with Mn. The LaMAlO:xMn,yMn phosphors show a narrow green emission band of the Mn ions at 514 nm and a red emission band of the Mn ions at 677 nm. In addition, the thermal stability of luminescence shows that the response of Mn and Mn to the temperature is obviously different in LaMAlO, implying the potential of the prepared phosphors as optical thermometers. The decay lifetime of Mn was changed with temperature due to the different fluorescence intensity ratios of Mn and Mn, and a dual-mode optical temperature-sensing mechanism was studied in the temperature range of -50-200 °C. The maximum relative sensitivities (S) are calculated as 3.22 and 3.13% K, respectively. The unique optical thermometric features demonstrate the application potential of LaMAlO:Mn,Mn in optical thermometry.
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http://dx.doi.org/10.1039/d1dt00153aDOI Listing
April 2021

Dexmedetomidine-soaked nasal packing can reduce pain and improve sleep quality after nasal endoscopic surgery: a double-blind, randomized, controlled clinical trial.

Sleep Breath 2021 Mar 12. Epub 2021 Mar 12.

Department of Anesthesiology, The First Affiliated Hospital of Shantou University Medical College, 57 Changping Road, Shan Tou, 515000, China.

Study Objective: Bilateral endoscopic nasal surgery is usually associated with pain and sleep disturbance. The aim of this study was to evaluate the effects of dexmedetomidine-soaked nasal packing on analgesia and improvement of sleep quality in patients undergoing this surgery.

Method: Eighty patients were enrolled and randomly allocated into 4 groups. At the end of surgery, dexmedetomidine-soaked nasal packings were applied to three groups with a dosage of 1 μg kg (D1), 2μg kg (D2), 4 μg kg (D4) and normal saline-soaked nasal packing (NS) was applied to a fourth group. The primary outcome was postoperative pain scores using a visual analog scale (VAS) recorded at six time points: before the surgery (T1); 2 h (T2), 8 h (T3), 24 h (T4), 48 h (T5) after surgery; and at the moment of nasal packing removal (T6). Secondary outcomes were postoperative sleep status evaluated by the Pittsburgh sleep quality index (PSQI) and subjective sleep quality value (SSQV). Factors affecting sleep, hemodynamic changes, and adverse events were also recorded.

Results: Compared with the NS group, dexmedetomidine-soaked nasal packing significantly relieved postoperative pain and improved sleep quality. The effect was similar between D2 and D4, which was greater than in D1. However, D2 was associated with fewer adverse events.

Conclusions: Dexmedetomidine-soaked nasal packing not only offers effective analgesia but also improves postoperative sleep quality in patients undergoing bilateral endoscopic nasal surgery. Taking effect and adverse events into consideration, a dosage of 2μg kg may be optimal.

Trial Registration: www.chictr.org.cn/index.aspx (ChiCTR1900025692) Retrospectively registered 5 September 2019.
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http://dx.doi.org/10.1007/s11325-021-02342-yDOI Listing
March 2021

Polybrominated diphenyl ethers quinone-induced intracellular protein oxidative damage triggers ubiquitin-proteasome and autophagy-lysosomal system activation in LO2 cells.

Chemosphere 2021 Feb 23;275:130034. Epub 2021 Feb 23.

Key Laboratory of Luminescence Analysis and Molecular Sensing, Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing, 400715, China; State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China. Electronic address:

Polybrominated diphenyl ethers (PBDEs), a kind of flame retardants, were widely used in the furniture, textile and electronics industries. Because of their lipophilic, persistent and bio-accumulative properties, PBDEs were listed on the Stockholm Convention as typical persistent organic pollutants (POPs). We have previously reported that a highly active, quinone-type metabolite of PBDEs (PBDEQ) causes DNA damage and subsequently triggers apoptosis. However, it is remaining unclear whether PBDEQ provokes protein damage and stimulates corresponding signaling cascade. Using human normal liver (LO2) cells as an in vitro model, we demonstrated that PBDEQ causes oxidative protein damage through excess reactive oxygen species (ROS). Consistently, we found PBDEQ exposure causes the depletion of protein thiol group, the appearance of carbonyl group and the accumulation of protein aggregates. Endoplasmic reticulum (ER) stress was involved in the repair of oxidized proteins. Under the scenario of severe damage, LO2 cells degrade oxidized proteins through ubiquitin-proteasome system (UPS) and autophagy. The blockage of these protein degradation pathways aggravates PBDEQ-induced cytotoxicity in LO2 cells, whilst antioxidant N-acetyl-cysteine (NAC) rescues PBDEQ-induced oxidative protein damage conversely. In summary, our current study first demonstrated PBDEQ-induced protein oxidative damage in LO2 cells, which offer a better understanding of the cytotoxicity of PBDEs and corresponding metabolites.
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http://dx.doi.org/10.1016/j.chemosphere.2021.130034DOI Listing
February 2021

Polypyrrole-functionalized magnetic BiMoO nanocomposites as a fast, efficient and reusable adsorbent for removal of ketoprofen and indomethacin from aqueous solution.

J Colloid Interface Sci 2021 Jun 13;592:51-65. Epub 2021 Feb 13.

Gansu Key Laboratory for Environmental Pollution Prediction and Control, College of Earth and Environmental Sciences, Lanzhou University, Lanzhou 730000, China. Electronic address:

Pharmaceutical and personal care products (PPCPs) as emerging organic pollutants have received widespread attention. A novel composite adsorbent was successfully prepared through the modification of polypyrrole (PPy) onto the surface of magnetic BiMoO (MnFeO/BiMoO/PPy) and acted as excellent adsorbent to remove organic compounds from aqueous solution. The adsorbent was characterized by SEM, TEM, FT-IR, XRD, BET, VSM, XPS and PES. The resulting composites combined the advantages of all three parts and possessed porous structure and magnetic properties, which showed excellent adsorption performance for ketoprofen (KET) and indomethacin (IDM), as evidenced by 87.03% and 86.24% of removal in just 120 min at initial concentration of 10 mg/L, respectively. The adsorption processes of MnFeO/BiMoO/PPy for PPCPs were well fitted with the pseudo-second order kinetic model and described better by Langmuir isotherm model. The possible adsorption mechanisms of KET and IDM by MnFeO/BiMoO/PPy were provided. Additionally, the effect of other coexisting substances was also explored using Cu as a model. Moreover, the adsorption performances were further studied to remove several dyes, in which showed superior affinity for anionic dyes. In practical application, it had almost equal removal capacity for KET both in the Yellow River water and sewage treatment plant inlet water.
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http://dx.doi.org/10.1016/j.jcis.2021.02.033DOI Listing
June 2021

Effects of Banana Resistant Starch on the Biochemical Indexes and Intestinal Flora of Obese Rats Induced by a High-Fat Diet and Their Correlation Analysis.

Front Bioeng Biotechnol 2021 27;9:575724. Epub 2021 Jan 27.

School of Food Science and Engineering, South China University of Technology, Guangzhou, China.

The effects of banana resistant starch (BRS) on obesity-related metabolic and intestinal flora were investigated in a high-fat diet-induced obesity model. After 6 weeks of intervention, the glucolipid metabolism index [blood glucose (GLU), total cholesterol (TC), triacylglycerol (TG), low density lipoprotein-cholesterol (LDL-C), and high density lipoprotein-cholesterol (HDL-C)], hormone index [leptin (LEP), insulin (INS), ghrelin, adiponectin (ADP), and thyroxine (T4)], and 16S rRNA sequencing analyses were performed for each group to explore the regulating effect of intestinal flora and the mechanism of weight loss in obese rats. The results showed that (1) BRS intervention significantly reduced the levels of GLU, TG, TC, LDL-C, LEP, and INS ( < 0.01) and increased the contents of ghrelin ( < 0.05) and ADP ( < 0.01). (2) BRS could improve the diversity of intestinal flora and regulate the overall structure of intestinal microorganisms, mainly by upregulating the / ratio and the relative abundance of and downregulating the relative abundances of and (at the phylum level). BRS could inhibit the proliferation of , , and and increase the abundances of , , and (at the genus level). (3) Some significant correlations were observed between the gut microbiota and biomarkers. , , and were positively correlated with GLU, TG, TC, LEP, and INS and negatively correlated with ghrelin and ADP. , , and were negatively correlated with GLU, TG, and TC. Conclusion: BRS had promising effects on weight loss, which could be associated with the improvement in host metabolism by regulating intestinal flora.
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http://dx.doi.org/10.3389/fbioe.2021.575724DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7873301PMC
January 2021

Gastric poorly cohesive carcinoma: differentiation from tubular adenocarcinoma using nomograms based on CT findings in the 40 s late arterial phase.

Eur Radiol 2021 Feb 10. Epub 2021 Feb 10.

Department of Radiology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, No. 321, Zhongshan Road, Nanjing City, 210008, Jiangsu Province, China.

Objectives: To summarise the CT findings of gastric poorly cohesive carcinoma (PCC) in the 40 s late arterial phase and differentiate it from tubular adenocarcinoma (TAC) using an integrative nomogram.

Methods: A total of 241 patients including 59 PCCs, 109 TACs, and 73 other type gastric cancers were enrolled. Thirteen CT morphological characteristics of each lesion in the late arterial phase were evaluated. In addition, CT value-related parameters were extracted from ROIs encompassing the area of greatest enhancement on four-phase CT images. Nomograms based on regression models were built to discriminate PCCs from TACs and from non-PCCs. ROC curve analysis was performed to assess the diagnostic efficiency.

Results: Six morphological characteristics, 10 CT value-related parameters, and the enhanced curve types differed significantly among the above three groups in the primary cohort (all p < 0.05). The paired comparison revealed that 10 CT value-related parameters differed significantly between PCCs and TACs (all p < 0.05). The AUC of the nomogram based on the multivariate model for discriminating PCCs from TACs was 0.954, which was confirmed in the validation cohort (AUC = 0.895). The AUC of another nomogram for discriminating PCCs from non-PCCs was 0.938, which was confirmed in the validation cohort (AUC = 0.880).

Conclusions: In the 40 s late arterial phase, the morphological characteristics and CT value-related parameters were significantly different among PCCs, TACs, and other types. PCCs were prone to manifest mucosal line interruption, diffuse thickening, infiltrative growth, and slow-rising enhanced curve (Type A). Furthermore, multivariate models were useful in discriminating PCCs from TACs and other types.

Key Points: • Multiple morphological characteristics and CT value-related parameters differed significantly between gastric PCCs and TACs in the 40 s late arterial phase. • The nomogram integrating morphological characteristics and CT value-related parameters in the 40 s late arterial phase had favourable performance in discriminating PCCs from TACs. • More useful information can be derived from 40 s late arterial phase CT images; thus, a more accurate evaluation can be made in clinical practice.
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http://dx.doi.org/10.1007/s00330-021-07697-0DOI Listing
February 2021

Analysis in statistical perspective about the article titled "Pre-emptive epidural analgesia for acute and chronic post-thoracotomy pain in adults: a systematic review and meta-analysis".

Reg Anesth Pain Med 2021 Feb 10. Epub 2021 Feb 10.

Teaching and Research Office of Oncology, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China

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http://dx.doi.org/10.1136/rapm-2020-102319DOI Listing
February 2021

An Open-label, Multicenter, Single-arm, Phase II Study of Fluzoparib in Patients with Germline Mutation and Platinum-sensitive Recurrent Ovarian Cancer.

Clin Cancer Res 2021 May 8;27(9):2452-2458. Epub 2021 Feb 8.

Department of Gynecologic Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, P.R. China.

Purpose: Fluzoparib (PARP inhibitor) showed promising antitumor activity for advanced ovarian cancer in a phase I study. This study aimed to assess the efficacy and safety of fluzoparib in patients with germline -mutated recurrent ovarian cancer.

Patients And Methods: This open-label, multicenter, single-arm, phase II study enrolled patients with platinum-sensitive recurrent ovarian cancer and germline mutation who had previously received two to four lines of platinum-based chemotherapy. Fluzoparib 150 mg was administered orally twice daily. The primary endpoint was independent review committee (IRC)-assessed objective response rate per RECIST v1.1.

Results: A total of 113 patients were enrolled and received at least one dose of fluzoparib. As of data cutoff on March 21, 2020, the median follow-up period was 15.9 months (interquartile range, 13.5-18.5). The IRC- and investigator-assessed objective response rates were 69.9% [95% confidence interval (CI), 60.6-78.2] and 70.8% (95% CI, 61.5-79.0), respectively. The objective response rates were similar across all prespecified subgroups. The median IRC- and investigator-assessed progression-free survival was 12.0 months (95% CI, 9.3-13.9) and 10.3 months (95% CI, 9.2-12.0), respectively. The 12-month survival rate was 93.7% (95% CI, 87.2-96.9). Grade ≥3 adverse events occurred in 63.7% (72/113) of the patients, with the most common one being anemia/decreased hemoglobin. Adverse events that led to treatment interruption, dose reduction, and discontinuation occurred in 39.8%, 34.5%, and 0.9% of patients, respectively. One treatment-related death occurred.

Conclusions: Fluzoparib demonstrated promising antitumor activity and acceptable safety profile in germline -mutated, platinum-sensitive relapsed ovarian cancer. Thus, fluzoparib might be a novel treatment option for this population.
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http://dx.doi.org/10.1158/1078-0432.CCR-20-3546DOI Listing
May 2021

The synthesis and biological evaluation of sanguinarine derivatives as anti-non-small cell lung cancer agents.

RSC Med Chem 2020 Feb 12;11(2):293-296. Epub 2020 Feb 12.

International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of Chinese Ministry of Education (MOE) , School of Pharmacy , Jinan University , 601 Huangpu Avenue West , Guangzhou 510632 , China . Email:

A novel series of sanguinarine (SA) derivatives were synthesized and evaluated as anti-non-small cell lung cancer (NSCLC) agents. The compounds inhibited A549 and H1975 NSCLC cells with IC values of 0.96 - >30 μM and 0.79 - >30 μM, respectively. Compounds exhibited low micromolar inhibitory activity and indicated that the C6-position of SA was tolerated to be substituted by hydrophilic groups and CN. Further investigation of their mechanism of action showed that compound induced apoptosis of A549 and H1975 cells by inhibiting the Akt signaling pathway and elevating the reactive oxygen species (ROS). This study provided a strategy for developing new anti-cancer agents.
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http://dx.doi.org/10.1039/c9md00494gDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7489057PMC
February 2020

Blue circularly polarized luminescent amorphous molecules with single-handed propeller chirality induced by circularly polarized light irradiation.

Chem Commun (Camb) 2021 Feb 21;57(14):1794-1797. Epub 2021 Jan 21.

Institute for Catalysis (ICAT) and Graduate School of Chemical Sciences and Engineering, Hokkaido University, N21W10, Kita-ku, Sapporo 001-0021, Japan.

Preferred-handed propeller conformation was induced by circularly polarized light irradiation to three amorphous molecules with trigonal symmetry, and the molecules with induced chirality efficiently exhibited blue circularly polarized luminescence. In both chirality induction and luminescence, chirality appeared to be amplified due to intermolecular interactions through π-stacking.
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http://dx.doi.org/10.1039/d0cc07898kDOI Listing
February 2021

Endoplasmic reticulum stress manipulates autophagic response that antagonizes polybrominated diphenyl ethers quinone induced cytotoxicity in microglial BV2 cells.

J Hazard Mater 2021 Jun 30;411:124958. Epub 2020 Dec 30.

Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University, 2 Tiansheng Rd, Beibei District, Chongqing 400715, China; State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. Electronic address:

Polybrominated diphenyl ethers (PBDEs) were widely used as flame retardants. Previously, we reported that their quinone-type metabolite (PBDEQ) induced selective autophagy, but its biological consequences remain obscure. Here, we illustrated the possible link of PBDEQ-induced autophagy with endoplasmic reticulum (ER) stress and cytotoxicity in microglial BV2 cells. We found PBDEQ increased the formation of autophagosomes, promoted autophagic degradation, suggesting an improved autophagy flux in BV2 cells. Interestingly, both pharmacologic autophagy inhibitors and autophagy-related 5 gene small interfering RNA (ATG5 siRNA) aggravated the cytotoxicity of PBDEQ, suggesting the antagonizing role of autophagy. PBDEQ induced ER stress and activated protein kinase R-like ER kinase (PERK)-eukaryotic translation initiation factor 2α (eIF2α)-activating transcription factor 4 (ATF4)-C/EBP homologous protein (CHOP) axis of classic unfolded protein response (UPR) pathway, whilst ER stress inhibitor blocked PBDEQ-induced autophagy. Moreover, N-acetyl-L-cysteine (NAC) alleviated PBDEQ-induced activation of ER stress and autophagy, suggesting reactive oxygen species (ROS) were involved in regulating PBDEQ-induced ER stress and autophagy. Taken together, our results demonstrate a new mechanism of PBDEQ-associated toxicity.
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http://dx.doi.org/10.1016/j.jhazmat.2020.124958DOI Listing
June 2021

Enhanced therapeutic efficacy of Listeria-based cancer vaccine with codon-optimized HPV16 E7.

Hum Vaccin Immunother 2021 Jun 15;17(6):1568-1577. Epub 2021 Jan 15.

Jiangsu Key Laboratory of Zoonosis, Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, MOA of China, Joint International Research Laboratory of Agriculture and Agri-Product Safety, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonosis, Yangzhou University, Yangzhou, Jiangsu Province, China.

Cervical cancer is a leading cause of high mortality in women in developing countries and has a serious impact on women's health. Human papilloma virus (HPV) prophylactic vaccines have been produced and may hold promise for reducing the incidence of cervical cancer. However, the limitations of current HPV vaccine strategies make the development of HPV therapeutic vaccines particularly important for the treatment of HPV related lesions. Our previous work has demonstrated that LM4Δ::E7 was safe and effective in inducing antitumor effect by antigen-specific cellular immune responses and direct killing of tumor cell on a cervical cancer model. In this study, the codon usage effect of a novel -based cervical cancer vaccine LM4Δ::E7-1, was evaluated for effects of codon-optimized E7 expression, cellular immune response and therapeutic efficacy in a tumor-bearing murine model. Our data demonstrated that up-regulated expression of E7 was strikingly elevated by codon usage optimization, and thus induced significantly higher Th1-biased immunity, lymphocyte proliferation, and strong specific CTL activity ex-vivo compared with LM4Δ::E7-treated mice. Furthermore, LM4Δ::E7-1 enhanced a remarkable therapeutic effect in establishing tumors. Taken together, our results suggest that codon usage optimization is an important consideration in constructing live bacterial-vectored vaccines and is required for promoting effective T cell responses.
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http://dx.doi.org/10.1080/21645515.2020.1839291DOI Listing
June 2021

Properties of Calmodulin Binding to Na1.2 IQ Motif and Its Autism-Associated Mutation R1902C.

Neurochem Res 2021 Mar 4;46(3):523-534. Epub 2021 Jan 4.

Department of Pharmaceutical Toxicology, School of Pharmacy, China Medical University, Shenyang, 110122, China.

Voltage-gated sodium channels (VGSCs) are fundamental to the initiation and propagation of action potentials in excitable cells. Ca/calmodulin (CaM) binds to VGSC type II (Na1.2) isoleucine and glutamine (IQ) motif. An autism-associated mutation in Na1.2 IQ motif, Arg1902Cys (R1902C), has been reported to affect the combination between CaM and the IQ motif compared to that of the wild type IQ motif. However, the detailed properties for the Ca-regulated binding of CaM to Na1.2 IQ (1901Lys-1927Lys, IQ) and mutant IQ motif (IQ) remains unclear. Here, the binding ability of CaM and CaM's constituent proteins including N- and C lobe to the IQ motif of Na1.2 and its mutant was investigated by protein pull-down experiments. We discovered that the combination between CaM and the IQ motif was U-shaped with the highest at [Ca] ≈ free and the lowest at 100 nM [Ca]. In the IQ mutant, Ca-dependence of CaM binding was nearly lost. Consequently, the binding of CaM to IQ at 100 and 500 nM [Ca] was increased compared to that of IQ. Both N- and C lobe of CaM could bind with Na1.2 IQ motif and IQ mutant, with the major effect of C lobe. Furthermore, CaMKII had no impact on the binding between CaM and Na1.2 IQ motif. This research offers novel insight to the regulation of Na1.2 IQ and IQ motif, an autism-associated mutation, by CaM.
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http://dx.doi.org/10.1007/s11064-020-03189-7DOI Listing
March 2021

Case Report: [F]PI2620 as a Tau Imaging Agent in Posterior Cortical Atrophy.

Front Neurol 2020 8;11:566667. Epub 2020 Dec 8.

Department of Neurology, Xuanwu Hospital, Capital Medical University, Beijing, China.

Posterior cortical atrophy (PCA) is widely considered as an atypical variant of Alzheimer disease and is characterized by a progressive decline in visual function. PCA has been investigated from the standpoints of brain structure and metabolism, but tau deposition and its relationship to disease severity still remain unclear. Here, we used a novel tau ligand, [F]PI2620, to visualize tau deposition in a PCA patient. The results showed that high [F]PI2620 uptake in posterior cortical regions was associated with clinical manifestations, morphologic changes in the brain observed by magnetic resonance imaging (MRI), and hypometabolism detected by [F] fluorodeoxyglucose (FDG) positron emission tomography (PET). This is the first report demonstrating a clinical anatomical correspondence between [F]PI2620 PET results, clinical manifestations, MRI, and [F]FDG PET findings in a Chinese patient with PCA. The results also support the utility of [F]PI2620 for visualizing tau aggregation in PCA.
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http://dx.doi.org/10.3389/fneur.2020.566667DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7753187PMC
December 2020

Qualitative and Quantitative Wall Enhancement Analyses in Unruptured Aneurysms Are Associated With an Increased Risk of Aneurysm Instability.

Front Neurosci 2020 10;14:580205. Epub 2020 Dec 10.

Department of Radiology, University of Washington, Seattle, WA, United States.

Objective: Intracranial aneurysm wall enhancement (AWE) is independently associated with unstable aneurysms. However, a quantitative analysis of wall enhancement is lacking. This study aims to investigate the relationship between qualitative and quantitative wall enhancement indices (WEIs), traditional risk factors for aneurysms, and clinical ELAPSS/PHASES scores in a large cohort of intracranial saccular aneurysms.

Materials And Methods: In this cross-sectional study, a total of 174 patients (mean age 60.4 ± 9.5 years; 53% women) with 248 asymptomatic unruptured intracranial aneurysms underwent pre- and post-contrast black-blood magnetic resonance imaging (MRI). The extent of AWE was defined as non-AWE (pattern 0), focal AWE (pattern 1), or circumferential AWE (pattern 2). WEI was calculated using wall signal intensities on pre- and post-contrast images. Predicted 3- and 5-year growth risk and 5-year rupture risk were obtained from ELAPSS and PHASES scores, respectively. Uni- and multivariate analyses were conducted to explore the relationship between AWE characteristics, risk-related factors, and aneurysm instability.

Results: Aneurysm size [odds ratio (OR), 1.3; 95% confidence interval (CI), 1.2-1.4; < 0.001], non-internal carotid artery/middle cerebral artery location (OR, 1.9; 95% CI, 1.0-3.6; = 0.045), and irregular shape (OR, 2.4; 95% CI, 1.2-4.5; = 0.009) were independently associated with AWE. For aneurysms with AWE, the estimated 3- and 5-year growth risk (25.3 ± 13.0% and 38.0 ± 17.4%) and the 5-year rupture risk (3.9 ± 5.2%) were 1.9-3.3 times higher than those for aneurysms without AWE (12.8 ± 9.1%, 20.3 ± 13.0%, and 1.2 ± 1.6%, respectively; all < 0.001). Larger areas and higher WEIs of enhancement positively correlated with aneurysm size ( = 0.43 and 0.38, respectively), 3- and 5-year growth risk, and 5-year rupture risk ( = 0.49 and 0.40, = 0.49 and 0.40, = 0.36 and 0.24, respectively; all < 0.001). In sum, a larger aneurysm size, non-internal carotid artery/middle cerebral artery location, and irregular shape were independently associated with AWE. Larger areas and higher WEIs were associated with an increased risk of aneurysm growth and rupture. These findings suggest that quantitative AWE metrics should be considered in future large-scale longitudinal studies to evaluate their value in aneurysm risk management.
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http://dx.doi.org/10.3389/fnins.2020.580205DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7758487PMC
December 2020

Transferrin receptor regulates malignancies and the stemness of hepatocellular carcinoma-derived cancer stem-like cells by affecting iron accumulation.

PLoS One 2020 22;15(12):e0243812. Epub 2020 Dec 22.

Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China.

Background: Iron metabolism is essential because it plays regulatory roles in various physiological and pathological processes. Disorders of iron metabolism balance are related to various cancers, including hepatocellular carcinoma. Cancer stem-like cells (CSCs) exert critical effects on chemotherapy failure, cancer metastasis, and subsequent disease recurrence and relapse. However, little is known about how iron metabolism affects liver CSCs. Here, we investigated the expression of transferrin receptor 1 (TFR1) and ferroportin (FPN), two iron importers, and an upstream regulator, iron regulatory protein 2 (IRP2), in liver hepatocellular carcinoma (LIHC) and related CSCs.

Methods: The expression levels of TFR1, FPN and IRP2 were analysed using the GEPIA database. CSCs were derived from parental LIHC cells cultured in serum-free medium. After TFR1 knockdown, ROS accumulation and malignant behaviours were measured. The CCK-8 assay was performed to detect cell viability after TFR1 knockdown and erastin treatment.

Results: TFR1 expression was upregulated in LIHC tissue and CSCs derived from LIHC cell lines, prompting us to investigate the roles of TFR1 in regulating CSCs. Knockdown of TFR1 expression decreased iron accumulation and inhibited malignant behaviour. Knockdown of TFR1 expression decreased reactive oxygen species (ROS) accumulation induced by erastin treatment and maintained mitochondrial function, indicating that TFR1 is critical in regulating erastin-induced cell death in CSCs. Additionally, knockdown of TFR1 expression decreased sphere formation by decreasing iron accumulation in CSCs, indicating a potential role for TFR1 in maintaining stemness.

Conclusion: These findings, which revealed TFR1 as a critical regulator of LIHC CSCs in malignant behaviour and stemness that functions by regulating iron accumulation, may have implications to improve therapeutic approaches.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0243812PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7755206PMC
January 2021

Qualitative and Quantitative Wall Enhancement on Magnetic Resonance Imaging Is Associated With Symptoms of Unruptured Intracranial Aneurysms.

Stroke 2021 01 22;52(1):213-222. Epub 2020 Dec 22.

Department of Radiology (M.R.L., M.M.-B., C.Z.), University of Washington, Seattle.

Background And Purpose: Aneurysmal wall enhancement (AWE) on vessel wall magnetic resonance imaging (VW-MRI) has been described as a new imaging biomarker of unstable unruptured intracranial aneurysms (UIAs). Previous studies of symptomatic UIAs are limited due to small sample sizes and lack of AWE quantification. Our study aims to investigate whether qualitative and quantitative assessment of AWE can differentiate symptomatic and asymptomatic UIAs.

Methods: Consecutive patients with UIAs were prospectively recruited for vessel wall magnetic resonance imaging at 3T from October 2014 to October 2019. UIAs were categorized as symptomatic if presenting with sentinel headache or oculomotor nerve palsy directly related to the aneurysm. Evaluation of wall enhancement included enhancement pattern (0=none, 1=focal, and 2=circumferential) and quantitative wall enhancement index (WEI). Univariate and multivariate analyses were used to identify the parameters associated with symptoms.

Results: Two hundred sixty-seven patients with 341 UIAs (93 symptomatic and 248 asymptomatic) were included in this study. Symptomatic UIAs more frequently showed circumferential AWE than asymptomatic UIAs (66.7% versus 17.3%, <0.001), as well as higher WEI (median [interquartile range], 1.3 [1.0-1.9] versus 0.3 [0.1-0.9], <0.001). In multivariate analysis, both AWE pattern and WEI were independent factors associated with symptoms (odds ratio=2.03 across AWE patterns [95% CI, 1.21-3.39], =0.01; odds ratio=3.32 for WEI [95% CI, 1.51-7.26], =0.003). The combination of AWE pattern and WEI had an area under the curve of 0.91 to identify symptomatic UIAs, with a sensitivity of 95.7% and a specificity of 73.4%.

Conclusions: In a large cohort of UIAs with vessel wall magnetic resonance imaging, both AWE pattern and WEI were independently associated with aneurysm-related symptoms. The qualitative and quantitative features of AWE can potentially be used to identify unstable intracranial aneurysms.
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http://dx.doi.org/10.1161/STROKEAHA.120.029685DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7770055PMC
January 2021

A comprehensive expression profile of tRNA-derived fragments in papillary thyroid cancer.

J Clin Lab Anal 2021 Mar 17;35(3):e23664. Epub 2020 Dec 17.

Department of General Surgery, The Affiliated Changzhou No.2 People's Hospital of Nanjing Medical University, Changzhou, China.

Background: The incidence of thyroid cancer has been on a rise. Papillary thyroid cancer (PTC) is the most common type of malignant thyroid tumor and accounts for approximately 85% of thyroid cancer cases. Although the genetic background of PTC has been studied extensively, relatively little is known about the role of small noncoding RNAs (sncRNAs) in PTC. tRNA-derived fragments (tRFs) represent a newly discovered class of sncRNAs that exist in many species and play key roles in various biological processes.

Methods: In this study, we used high-throughput next-generation sequencing technology to analyze the expression of tRFs in samples from PTC tissues and normal tissues. We selected four tRFs to perform qPCR to determine the expression levels of these molecules and make bioinformatic predictions.

Results: We identified 53 unique tRFs and transfer RNA halves (tsRNAs). The 10 most upregulated tRFs and tsRNAs were tRF-39-I6D3887S1RMH5MI2, tRF-21-2E489B3RB, tRF-18-JMRPFQDY, tRF-17-202L2YF, tRF-17-VBY9PYJ, tRF-18-YRRHQFD2, tRF-21-WE884U1DD, tRF-41-EX2Z10I9BZBZOS4YB, tRF-39-HPDEXK7S1RNS9MI2, and tRF-20-1SS2P46I. The 10 most downregulated tRFs and tsRNAs were tRF-31-HQ9M739P8WQ0B, tRF-43-5YXENDBP1IUUK7VZV, tRF-38-RZYQHQ9M739P8WD8, tRF-25-9M739P8WQ0, tRF-33-V6Z3M8ZLSSXUD6, tRF-27-MY73H3RXPLM, tRF-26-DBNIB9I1KQ0, tRF-38-Z9HMI8W47W1R7HX, tRF-40-Z6V6Z3M8ZLSSXUOL, and tRF-39-YQHQ9M739P8WQ0EB. qPCR verification of cell lines and tissue samples yielded results consistent with the sequencing analysis. As tRF-39 expression showed the maximum difference between PTC cells and normal cells, we chose this tRF to predict targets and perform functional tRF and tsRNA enrichment analysis.

Conclusion: In this study, we provided a comprehensive catalog of tRFs involved in PTC and assessed the abnormal expression of these fragments. Through qPCR verification, tRF-39-0VL8K87SIRMM12E2 was found to be the most significantly upregulated tRF. Further tRF and enrichment analysis revealed that tRF-39 was mostly enriched in the "metabolic pathways." These preliminary findings can be used as the basis for further research studies based on the functional role of tRFs in patients with PTC.
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http://dx.doi.org/10.1002/jcla.23664DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7957983PMC
March 2021