Publications by authors named "Yuting Mei"

5 Publications

  • Page 1 of 1

Generation of an induced pluripotent stem cell line from a patient with global development delay carrying DYRK1A mutation (c.1730T>A) and a gene correction isogenic iPSC line.

Stem Cell Res 2021 Mar 20;53:102305. Epub 2021 Mar 20.

Stem Cell Research Center, Institute of Pediatrics, Children's Hospital, Fudan University, 399 Wanyuan Road, Shanghai 201102, China; Key Laboratory of Birth Defects, Children's Hospital of Fudan University, Shanghai, China. Electronic address:

Mental retardation autosomal dominant 7 (MRD7), or DYRK1A Related Intellectual Disability Syndrome (OMIM 614104) is a developmental syndrome with microcephaly, intellectual disability, language delay and epileptic seizures. Haploinsufficiency of DYRK1A is the cause of MRD7. Here, we generated an induced pluripotent stem cell (iPSC) line with the mutation (DYRK1Ac.1730T>A) from the Peripheral blood mononuclear cell (PBMC) of a MRD7 patient along with an isogenic gene-corrected control iPSC line by CRISPR/Cas9 genome editing. Both iPSC lines showed full pluripotency, normal karyotype and differentiation capacity without integrating vectors. These DYRK1A mutant and isogenic gene-corrected iPSC control line provides a useful model to study the underlying molecular mechanisms of MRD7.
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http://dx.doi.org/10.1016/j.scr.2021.102305DOI Listing
March 2021

Transcriptional networks identify synaptotagmin-like 3 as a regulator of cortical neuronal migration during early neurodevelopment.

Cell Rep 2021 Mar;34(9):108802

Stem Cell Center, Children's Hospital of Fudan University, Shanghai 201102, China. Electronic address:

Human brain development is a complex process involving neural proliferation, differentiation, and migration that are directed by many essential cellular factors and drivers. Here, using the NetBID2 algorithm and developing human brain RNA sequencing dataset, we identify synaptotagmin-like 3 (SYTL3) as one of the top drivers of early human brain development. Interestingly, SYTL3 exhibits high activity but low expression in both early developmental human cortex and human embryonic stem cell (hESC)-derived neurons. Knockout of SYTL3 (SYTL3-KO) in human neurons or knockdown of Sytl3 in embryonic mouse cortex markedly promotes neuronal migration. SYTL3-KO causes an abnormal distribution of deep-layer neurons in brain organoids and reduces presynaptic neurotransmitter release in hESC-derived neurons. We further demonstrate that SYTL3-KO-accelerated neuronal migration is modulated by high expression of matrix metalloproteinases. Together, based on bioinformatics and biological experiments, we identify SYTL3 as a regulator of cortical neuronal migration in human and mouse developing brains.
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http://dx.doi.org/10.1016/j.celrep.2021.108802DOI Listing
March 2021

Assessment of Ecosystem Service Quality and Its Correlation with Landscape Patterns in Haidian District, Beijing.

Int J Environ Res Public Health 2019 04 8;16(7). Epub 2019 Apr 8.

Orirent Landscape, IT Industrial Park, 104 Floor, 10 Courtyard Electronic City, Jiuxianqiao North Road, Chaoyang District, Beijing 100015, China.

Landscape architecture with urban green space as the main research object is an evidence-based science. It is an important issue to optimize green space systems from the point of view of ecosystem services. In this paper, high-resolution (1.5 m resolution) remote sensing images are combined with data-processing software, such as ENVI, ArcGIS, and Fragstats, to evaluate ecosystem service quality and compute the landscape pattern in the Haidian District (Beijing, China), so that the relationship between the ecosystem service quality and landscape pattern can be quantitatively studied and a strategy can be provided for green space optimization in cities. The following conclusions are drawn: (1) for the evaluated quality of 14 ecosystem services in Haidian District (refer to Section Analysis of the Association of the Percentage of Patches (PLAND) Index of Forest Land and Quality of Ecosystem Service in Haidian District). Forest land is the main provider of the ecosystem service in Haidian District, while construction land only provides cultural services; (2) on the whole, the spatial distribution of the ecosystem services in Haidian District gradually decreases from the west to the east, which basically matches with the spatial distribution of the forest land. The regulating service and supporting service are matched with the distribution of the urban green space. The cultural service is closely associated with history resource points; and (3) the analysis results of the association between landscape pattern and ecosystem service quality show that the percentage of patches (PLAND) index for forest land has a significant logarithmic relationship with the regulating service and supporting service. The critical value of the PLAND index is 30. Besides the Xishan area with the most coverage of forest land, the landscape shape index (LSI) of the brushwood has a logarithmic relationship with the ecosystem service quality. The critical value of the LSI value is 50. Finally, this paper proposes an area optimization strategy of green space in Haidian District from the view of the ecosystem system service. The Xishan area is classified into the ecosystem red line to control city expansion. The regulating and supporting services can be enhanced in the north flat area by improving the patch shape index. The ecosystem service capabilities can be improved by adding the forest land in the existing green space for the southeast urban areas.
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http://dx.doi.org/10.3390/ijerph16071248DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6479810PMC
April 2019

Effect of transglutaminase on rennet-induced gelation of skim milk and soymilk mixtures.

J Sci Food Agric 2019 Mar 12;99(4):1820-1827. Epub 2018 Nov 12.

Beijing Laboratory for Food Quality and Safety, Beijing Key Laboratory of Agricultural Product Detection and Control for Spoilage Organisms and Pesticides, Food Science and Engineering College, Beijing University of Agriculture, Beijing, P. R. China.

Background: Protein gels made from cow milk and soymilk can yield products of exceptional value. Transglutaminase (TG) affect rennet-induced gelation of proteins, and improves the functionality of the final products. In this paper, TG and rennet were added to skim milk and soymilk mixtures simultaneously, and the rennet-induced coagulation was studied. Diffusing wave spectroscopy and rheology measurements were used to access the structural changes of the mixtures during renneting. Syneresis analysis and microscopy can give more information for understanding the system.

Results: Soymilk and TG have synergetic effects and inhibit rennet-induced gelation to a certain degree. With increasing soymilk and TG, elastic index and storage modulus decreased, gelation time was delayed, and curd yield and moisture content increased. At excess soymilk and TG, no curds can be formed. There were significant effects of soymilk and TG on curd microstructure. Soymilk inhibited the aggregation of casein micelles and contributed to more coarse and heterogeneous networks. TG limited reorganization of the proteins, leading to more homogenous networks with small pores.

Conclusion: The use of soymilk and TG simultaneously impair rennet-induced gelation and curd syneresis, and consequently lead to a higher yield of high-moisture curd. © 2018 Society of Chemical Industry.
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http://dx.doi.org/10.1002/jsfa.9375DOI Listing
March 2019

Spatiotemporal Patterns of the Use of Urban Green Spaces and External Factors Contributing to Their Use in Central Beijing.

Int J Environ Res Public Health 2017 02 27;14(3). Epub 2017 Feb 27.

School of Landscape Architecture, Beijing Forestry University, Beijing 100083, China.

Urban green spaces encourage outdoor activity and social communication that contribute to the health of local residents. Examining the relationship between the use of urban green spaces and factors influencing their utilization can provide essential references for green space site selection in urban planning. In contrast to previous studies that focused on internal factors, this study highlights the external factors (traffic convenience, population density and commercial facilities) contributing to the use of urban green spaces. We conducted a spatiotemporal analysis of the distribution of visitors in 208 selected green spaces in central Beijing. We examined the relationship between the spatial pattern of visitor distribution within urban green spaces and external factors, using the Gini coefficient, kernel density estimation, and geographical detectors. The results of the study were as follows. The spatial distribution of visitors within central Beijing's green spaces was concentrated, forming different agglomerations. The three examined external factors are all associated with the use of green spaces. Among them, commercial facilities are the important external factor associated with the use of green spaces. For the selection of sites for urban green spaces, we recommend consideration of external factors in order to balance urban green space utilization.
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http://dx.doi.org/10.3390/ijerph14030237DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5369073PMC
February 2017