Publications by authors named "Yuting Hu"

61 Publications

Maternal Nicotine Exposure Alters Hippocampal Microglia Polarization and Promotes Anti-inflammatory Signaling in Juvenile Offspring in Mice.

Front Pharmacol 2021 11;12:661304. Epub 2021 May 11.

Center for Medical Research, School of Medicine, Anhui University of Science and Technology, Huainan, China.

Accumulating evidence reveal that maternal smoking or perinatal nicotine replacement therapy impairs hippocampal neurogenesis, neural development, and cognitive behaviors in the offspring. Microglia is a source of non-neural regulation of neuronal development and postnatal neurogenesis. In this study, we explored the impact of nicotine on the microglia during the development of hippocampus. Developmental nicotine exposure in a mouse model was conducted by supplementing nicotine in the drinking water to mother mice during gestation and lactation period. We found that juvenile offspring with maternal nicotine exposure presented physical and neurobehavioral development delay and an increase in anxiety-like behavior in the open field test on postnatal day (PND) 20. To further detect possible developmental neurotoxic effects of nicotine in offspring and underlying mechanism, whole genome microarray analysis of the expression profile of the hippocampus was performed on postnatal day 20. Significant alterations in the expression of genes related to inflammatory, neurotransmitter, and synapsis were observed in the hippocampus after maternal nicotine exposure, as compared to the vehicle control. Concurrently, an increase in microglial markers and the presence of M2 polarity state in the hippocampus of the nicotine offspring were observed by histological analysis and confocal z-stacking scanning. The M2 microglial polarization state was further confirmed with primary microglia culture by cytokine array, and double-positive expression of BDNF/Iba1 in microglia by immunohistochemical staining in the juvenile offspring hippocampus was visualized. We also found that nicotine offspring showed an increase of neurite length in the molecular layer and CA1 by Tuj1 staining, as well as an increase in the expression of synapse associated protein, PSD95, but the expression of NeuroD1 in CA1 and CA3 reduced. In summary, maternal nicotine exposure dysregulates immune-related genes expression by skewing the polarity of M2 microglia in the hippocampus, which may cause abnormal cognitive and behavioral performance in the offspring.
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http://dx.doi.org/10.3389/fphar.2021.661304DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144443PMC
May 2021

Exploratory Investigation of Intestinal Structure and Function after Stroke in Mice.

Mediators Inflamm 2021 15;2021:1315797. Epub 2021 Feb 15.

Department of Gastroenterology, Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzhou 310052, China.

Stroke is the second leading cause of death worldwide. Patients who have a stroke are susceptible to many gastrointestinal (GI) complications, such as dysphagia, GI bleeding, and fecal incontinence. However, there are few studies focusing on the GI tract after stroke. The current study is to investigate the changes of intestinal structure and function in mice after ischemic stroke. Ischemic stroke was made as a disease model in mice, in which brain and ileal tissues were collected for experiments on the 1 and 7 day after stroke. Intestinal motility of mice was inhibited, and intestinal permeability was increased after stroke. Hematoxylin-eosin (HE) staining showed the accumulation of leucocytes in the intestinal mucosa. Myeloperoxidase (MPO) activity and inflammatory proteins (nuclear factor kappa-B (NF-B), inducible nitric oxide synthase (iNOS)) in the small intestine were significantly increased in mice after stroke. The expression of tight junction (TJ) proteins (zonula occludens-1 (ZO-1), occludin, and claudin-1) was downregulated, and transmission electron microscopy (TEM) showed broken TJ of the intestinal mucosa after stroke. Glial fibrillary acidic protein (GFAP) and the apoptosis-associated proteins (tumor necrosis factor (TNF-), caspase-3, and cleaved caspase-3) were notably upregulated as well. Ischemic stroke led to negative changes on intestinal structure and function. Inflammatory mediators and TNF--induced death receptor signaling pathways may be involved and disrupt the small intestinal barrier function. These results suggest that stroke patients should pay attention to GI protection.
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http://dx.doi.org/10.1155/2021/1315797DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7902147PMC
February 2021

Water-Sculpting of a Heterogeneous Nanoparticle Precatalyst for Mizoroki-Heck Couplings under Aqueous Micellar Catalysis Conditions.

J Am Chem Soc 2021 Mar 25;143(9):3373-3382. Epub 2021 Feb 25.

Department of Chemistry and Biochemistry, University of California, Santa Barbara, California 93106, United States.

Powdery, spherical nanoparticles (NPs) containing ppm levels of palladium ligated by -BuP, derived from FeCl, upon simple exposure to water undergo a remarkable alteration in their morphology leading to nanorods that catalyze Mizoroki-Heck (MH) couplings. Such NP alteration is general, shown to occur with three unrelated phosphine ligand-containing NPs. Each catalyst has been studied using X-ray photoelectron spectroscopy (XPS), energy-dispersive X-ray spectroscopy (EDX), transmission electron microscopy (TEM), and cryogenic transmission electron microscopy (cryo-TEM) analyses. Couplings that rely specifically on NPs containing -BuP-ligated Pd occur under aqueous micellar catalysis conditions between room temperature and 45 °C, and show broad substrate scope. Other key features associated with this new technology include low residual Pd in the product, recycling of the aqueous reaction medium, and an associated low E Factor. Synthesis of the precursor to galipinine, a member of the Hancock family of alkaloids, is suggestive of potential industrial applications.
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http://dx.doi.org/10.1021/jacs.0c11484DOI Listing
March 2021

High-density genetic map and QTL mapping for body color of the yellow catfish (Tachysurus fulvidraco♀ × T. vachellii♂).

Anim Genet 2021 Apr 27;52(2):246-248. Epub 2021 Jan 27.

Anhui Key Laboratory of Aquaculture and Stock Enhancement, Fisheries Research Institution, Anhui Academy of Agricultural Sciences, No.40, Nongkenan road, Hefei, Anhui, 230031, China.

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http://dx.doi.org/10.1111/age.13042DOI Listing
April 2021

Comprehensive preimplantation genetic testing by massively parallel sequencing.

Hum Reprod 2021 01;36(1):236-247

The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Study Question: Can whole genome sequencing (WGS) offer a relatively cost-effective approach for embryonic genome-wide haplotyping and preimplantation genetic testing (PGT) for monogenic disorders (PGT-M), aneuploidy (PGT-A) and structural rearrangements (PGT-SR)?

Summary Answer: Reliable genome-wide haplotyping, PGT-M, PGT-A and PGT-SR could be performed by WGS with 10× depth of parental and 4× depth of embryonic sequencing data.

What Is Known Already: Reduced representation genome sequencing with a genome-wide next-generation sequencing haplarithmisis-based solution has been verified as a generic approach for automated haplotyping and comprehensive PGT. Several low-depth massively parallel sequencing (MPS)-based methods for haplotyping and comprehensive PGT have been developed. However, an additional family member, such as a sibling, or a proband, is required for PGT-M haplotyping using low-depth MPS methods.

Study Design, Size, Duration: In this study, 10 families that had undergone traditional IVF-PGT and 53 embryos, including 13 embryos from two PGT-SR families and 40 embryos from eight PGT-M families, were included to evaluate a WGS-based method. There were 24 blastomeres and 29 blastocysts in total. All embryos were used for PGT-A. Karyomapping validated the WGS results. Clinical outcomes of the 10 families were evaluated.

Participants/materials, Setting, Methods: A blastomere or a few trophectoderm cells from the blastocyst were biopsied, and multiple displacement amplification (MDA) was performed. MDA DNA and bulk DNA of family members were used for library construction. Libraries were sequenced, and data analysis, including haplotype inheritance deduction for PGT-M and PGT-SR and read-count analysis for PGT-A, was performed using an in-house pipeline. Haplotyping with a proband and parent-only haplotyping without additional family members were performed to assess the WGS methodology. Concordance analysis between the WGS results and traditional PGT methods was performed.

Main Results And The Role Of Chance: For the 40 PGT-M and 53 PGT-A embryos, 100% concordance between the WGS and single-nucleotide polymorphism (SNP)-array results was observed, regardless of whether additional family members or a proband was included for PGT-M haplotyping. For the 13 embryos from the two PGT-SR families, the embryonic balanced translocation was detected and 100% concordance between WGS and MicroSeq with PCR-seq was demonstrated.

Limitations, Reasons For Caution: The number of samples in this study was limited. In some cases, the reference embryo for PGT-M or PGT-SR parent-only haplotyping was not available owing to failed direct genotyping.

Wider Implications Of The Findings: WGS-based PGT-A, PGT-M and PGT-SR offered a comprehensive PGT approach for haplotyping without the requirement for additional family members. It provided an improved complementary method to PGT methodologies, such as low-depth MPS- and SNP array-based methods.

Study Funding/competing Interest(s): This research was supported by the research grant from the National Key R&D Program of China (2018YFC0910201 and 2018YFC1004900), the Guangdong province science and technology project of China (2019B020226001), the Shenzhen Birth Defect Screening Project Lab (JZF No. [2016] 750) and the Shenzhen Municipal Government of China (JCYJ20170412152854656). This work was also supported by the National Natural Science Foundation of China (81771638, 81901495 and 81971344), the National Key R&D Program of China (2018YFC1004901 and 2016YFC0905103), the Shanghai Sailing Program (18YF1424800), the Shanghai Municipal Commission of Science and Technology Program (15411964000) and the Shanghai 'Rising Stars of Medical Talent' Youth Development Program Clinical Laboratory Practitioners Program (201972). The authors declare no competing interests.

Trial Registration Number: N/A.
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http://dx.doi.org/10.1093/humrep/deaa269DOI Listing
January 2021

A Novel Repair Idea on Nasal Sidewall Defect in Asians: Local Tissue Regeneration.

J Craniofac Surg 2020 Nov 16. Epub 2020 Nov 16.

Department of Plastic and Cosmetic Surgery, The Affiliated Friendship Plastic Surgery Hospital of Nanjing Medical University, Nanjing, Jiangsu, China.

Background: The traditional ways indicate using different types of flap to repair the nasal sidewall defect, but the scar in the donor area or bloated flap is a problem that cannot be ignored. This study propose the clinical application of the principle of tissue regeneration priority in small defects (defect diameter <1.0 cm) of nasal sidewall.

Methods: A retrospective study of 3 patients experienced the tumor excision and tissue regeneration healing in situ from January 2019 to January 2020. In this group, the nasal sidewall defect was treated with wound moist theory, preventing infection, to promote the wound regeneration and repair.

Result: The longest follow-up time was 8 month, all patient undergone half a year follow-up at least. One of them were left small sunken scars but all of them healed up well and satisfied with the results.

Conclusion: When dealing with the small defect of the skin and soft tissue on the nasal sidewall (defect diameter <1.0 cm), this article provided a new idea that is application of the priority principle for tissue regeneration repair rather than traditional flap used for 1-stage repair. For the small defects of the skin and soft tissue on the nasal sidewall, the tissue regeneration and natural repair in situ can achieve satisfactory effects. More importantly, it has the advantages of simplicity, ease of operation, and fewer complications.
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http://dx.doi.org/10.1097/SCS.0000000000007223DOI Listing
November 2020

Severe neonatal anemia affected by massive fetomaternal hemorrhage: a single-center retrospective observational study.

J Matern Fetal Neonatal Med 2020 Nov 12:1-7. Epub 2020 Nov 12.

Department of Gastrointestinal Lab, Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzhou, China.

Objectives: Massive fetomaternal hemorrhage (FMH) is a rare but sometimes life-threatening event, and surviving neonates may suffer major neurological complications. Severe neonatal anemia (SNA) affected by massive FMH is less reported in the literature. This study aims to explore the clinical characteristics, laboratory diagnoses, treatments and outcomes of SNA affected by massive FMH.

Methods: Data were collected retrospectively from the hospital's electronic medical record system. All neonates born in the hospital and admitted to the neonatal unit diagnosed as SNA affected by massive FMH from 1 January 2013 to 31 June 2017 were included.

Results: A total of 8 cases of SNA affected by FMH were identified among 6825 neonates admitted to the neonatal unit. They all presented with pallor but without hydrops at birth. Median gestational age and birthweight were 37 (36‒40) weeks and 2,625 (2300‒3050) g, respectively. Median hemoglobin level was 39.5 (25‒53) g/L at birth and 109.5 (94-127) g/L at discharge. Median maternal serum alpha-fetoprotein (AFP) was 3958.5 (1606‒14,330) ng/mL, which was significantly increased. Three out of eight cases manifested as antenatal decreased fetal movement. Only 1 with the lowest initial hemoglobin 25 g/L manifested as characteristic sinusoidal fetal heart rate tracing and suffered severe neonatal asphyxia and hypovolemic shock. Having experienced resuscitation, he was admitted to the neonatal unit and received twice transfusion of cross-matched red blood cells there. Another case with the initial hemoglobin 45 g/L received positive pressure ventilation and once transfusion. All cases were successfully discharged with a median hospital stay of 8 (5-12) days. Follow-up was available for 6 (75%) of 8 neonates (age range 13 months to 50 months), and all infants were observed to be in good condition with normal neurological status. In our series of eight cases, there were no neonatal deaths.

Conclusion: This study strengthens the idea that maternal AFP testing is valuable to confirm massive fetomaternal hemorrhage. Surviving neonates of massive FMH might have a good outcome despite severe anemia at birth.
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http://dx.doi.org/10.1080/14767058.2020.1845313DOI Listing
November 2020

Intranasal oxytocin attenuates insula activity in response to dynamic angry faces.

Biol Psychol 2020 11 4;157:107976. Epub 2020 Nov 4.

Key Laboratory of Cognition and Personality of Ministry of Education, Faculty of Psychology, Southwest University, Chongqing, 400715, China. Electronic address:

The effects of intranasal oxytocin on amygdala activity during emotional perception are often mixed. Given that the brain is organized into networks of interconnected areas, functional connectivity might provide an effective way to further understand the oxytocin effect. The aim of the present study was to investigate whether oxytocin administration affects amygdala activity and its functional connectivity during dynamic facial expression perception. Using a between-group, double-blind, placebo-controlled design, 55 participants were randomly assigned to groups receiving a single dose of 24 IU oxytocin or a placebo via intranasal administration. An implicit emotional task was employed to investigate the effect of oxytocin on neural responses to dynamic angry, neutral, and happy facial expressions with fMRI. Participants were instructed to respond only when the inverted dynamic faces were presented. The results indicated that oxytocin attenuated activation of insula and emotional processing-related regions (e.g., ACC, thalamus, and MFG) during the viewing of dynamic angry faces. However, functional connectivity between the regions involved in the perception of dynamic angry faces was not changed following oxytocin administration. The present findings may contribute to our understanding of the anxiolytic effects of oxytocin and eventually facilitate human clinical applications.
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http://dx.doi.org/10.1016/j.biopsycho.2020.107976DOI Listing
November 2020

Time is of essence - Abnormal time perspectives mediate the impact of childhood trauma on depression severity.

J Psychiatr Res 2021 05 31;137:534-541. Epub 2020 Oct 31.

Center for Cognition and Brain Disorders, The Affiliated Hospital of Hangzhou Normal University, Hangzhou, China; Hangzhou Seventh People's Hospital, Hangzhou, Zhejiang, China; Mental Health Center, Zhejiang University School of Medicine, Hangzhou, China; Institute of Mental Health Research, University of Ottawa, Ottawa, Canada.

Background: Major depressive disorder (MDD) is a multifaceted mental disorder where participants, in addition to various symptoms, often suffer from increased focus on past time perspective and higher incidence of childhood trauma. Whether the abnormal time perspective is a result of the depressive symptoms or, alternatively, mediates between childhood trauma and depression remains unclear.

Aims: To examine the triangular relationship between early life trauma, time perspective, and depressive symptoms.

Method: We investigated a large-scale MDD sample (n = 93) and healthy subject sample (n = 69) with Beck depression inventory-II (BDI-II), Beck hopelessness scale (BHS), childhood traumatic questionnaire-Short Form (CTQ-SF), and the Zimbardo time perspective inventory (ZTPI).

Results: The MDD patients reported more childhood trauma experiences, and were featured by abnormal time perspectives on past and future when compared to healthy control. By applying two alternative mediation models, we observed that the time perspective acted as a mediator between childhood trauma and depressive symptom severity, rather than as a consequence of depressive symptom. Furthermore, this abnormal time perspective was a risk factor to MDD, as the childhood trauma only mediated the time perspective in MDD. Finally, we showed that time perspective was a long-term personal trait and unchanged after the remission of depression symptoms under five-day transcranial magnetic stimulation.

Conclusions: Abnormal time perspective mediates the impact of early childhood trauma on depressive symptomatology. Besides better understanding of the temporal basis of depressive symptoms, we highlight the importance of preventive time perspective therapy in subjects with childhood trauma.
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http://dx.doi.org/10.1016/j.jpsychires.2020.10.039DOI Listing
May 2021

Toll-like receptor-2 gene knockout results in neurobehavioral dysfunctions and multiple brain structural and functional abnormalities in mice.

Brain Behav Immun 2021 01 16;91:257-266. Epub 2020 Oct 16.

Department of Neurology, Affiliated Hospital of Xuzhou Medical University, China; Institute of Neurological Diseases, Xuzhou Medical University, China. Electronic address:

Objective: Toll-like receptor-2 (TLR2), a member of TLR family, plays an important role in the induction and regulation of immune/inflammation. TLR2 gene knockout (TLR2KO) mice have been widely used for animal models of neurological diseases. Since there is close relationship between immune system and neurobehavioral functions, it is important to clarify the exact role of TLR2 defect itself in neurobehavioral functions. The present study aimed to investigate the effect of TLR2KO on neurobehavioral functions in mice and the mechanisms underlying the observed changes.

Methods: Male TLR2KO and wild type (WT) mice aged 3, 7, and 12 months were used for neurobehavioral testing and detection of protein expression by Western blot. Brain magnetic resonance imaging (MRI), electrophysiological recording, and Evans blue (EB) assay were applied to evaluate regional cerebral blood flow (rCBF), synaptic function, and blood-brain barrier (BBB) integrity in 12-month-old TLR2KO and age-matched WT mice.

Results: Compared to WT mice, TLR2KO mice showed decreased cognitive function and locomotor activity, as well as increased anxiety, which developed from middle age (before 7-month-old) to old age. In addition, significantly reduced regional cerebral blood flow (rCBF), inhibited long-term potentiation (LTP), and increased blood-brain barrier (BBB) permeability were observed in 12-month-old TLR2KO mice. Furthermore, compared with age-matched WT mice, significant reduction in protein levels of tight junction proteins (ZO-1, Occludin, and Claudin-5) and increased neurofilament protein (SMI32) were observed in 7 and 12-month-old TLR2KO mice, and that myelin basic protein (MBP) decreased in 12-month-old TLR2KO mice.

Conclusion: Our data demonstrated that TLR2 defect resulted in significantly observable neurobehavioral dysfunctions in mice starting from middle age, as well as multiple abnormalities in brain structure, function, and molecular metabolism.
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http://dx.doi.org/10.1016/j.bbi.2020.10.004DOI Listing
January 2021

Calculation of evapotranspiration in different climatic zones combining the long-term monitoring data with bootstrap method.

Environ Res 2020 12 14;191:110200. Epub 2020 Sep 14.

State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China. Electronic address:

Evapotranspiration (ET) is a central process in the climate system that plays a crucial role in the regional water cycle and climate regulation. However, estimating the effects of regional ET on the regional water cycle and climate regulation remains challenging due to the lack of quantitative methods and large-scale direct observational data. This study develops a new method to estimate evapotranspiration at regional scales using long-term monitoring data and the bootstrap resampling approach to calculate the ET unit area per year for China. This study applies the deviance information criterion as a goodness-of-fit index to select the most optimal formula for estimating regional ET for different climatic zones in China. The bootstrap resampling method was used to estimate parameter distribution in different climatic zones based on the outcome of 2000 trials. The results show that the predicted ET of adjacent climates overlaps with each other. The subtropical monsoonal climatic zone had the widest range of predicted ET (0-8000 mm/year), followed by the temperate and monsoonal climatic zones (0-1500 mm/year), mountain plateau climatic zone (0-1000 mm/year), and temperate continental climatic zone (0-500 mm/year). The probability distributions and isopleths of regionally predicted ET were also determined for China. The methods used in this study provide a promising tool to assess the effects of introducing large-scale forestation or restoration of trees on local water resources management.
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http://dx.doi.org/10.1016/j.envres.2020.110200DOI Listing
December 2020

Combined toxic effects of CBNPs and Pb on rat alveolar macrophage apoptosis and autophagy flux.

Ecotoxicol Environ Saf 2020 Dec 22;205:111062. Epub 2020 Aug 22.

College of Food Science and Engneering, Jilin University, Changchun, Jilin, 130062, People's Republic of China. Electronic address:

Carbon black (CB) and heavy metals are the main components of Particulate Matter (PM). Although the individual toxicities of CB and heavy metals have been extensively studied, the combined toxicity is much less understood. In this study, we choose the nano carbon black (CBNPs) and Pb to simulate fine particles in the atmosphere and study the combined toxic effect on rat alveolar macrophages. The data showed that CBNPs could adsorb Pb to form CBNPs-Pb complex and displayed an altered physical properties by particle characterization. CBNPs-Pb synergistically induced rat alveolar macrophages apoptosis and blocked autophagy flux compared with CBNPs and Pb individually. Consistent with this, CBNPs-Pb could impair the mitochondrial membrane potential (MMP), activate apoptotic signaling pathways, inhibit lysosomal function.
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http://dx.doi.org/10.1016/j.ecoenv.2020.111062DOI Listing
December 2020

Potential Role of Photosynthesis in the Regulation of Reactive Oxygen Species and Defence Responses to f. sp. in Wheat.

Int J Mol Sci 2020 Aug 11;21(16). Epub 2020 Aug 11.

Provincial Key Laboratory of Plant Breeding and Genetics, College of Agronomy, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.

Photosynthesis is not only a primary generator of reactive oxygen species (ROS) but also a component of plant defence. To determine the relationships among photosynthesis, ROS, and defence responses to powdery mildew in wheat, we compared the responses of the -expressing wheat line L658 and its susceptible sister line L958 at 0, 6, 12, 24, 48, and 72 h post-inoculation (hpi) with powdery mildew via analyses of transcriptomes, cytology, antioxidant activities, photosynthesis, and chlorophyll fluorescence parameters. The results showed that HO accumulation in L658 was significantly greater than that in L958 at 6 and 48 hpi, and the enzymes activity and transcripts expression of peroxidase and catalase were suppressed in L658 compared with L958. In addition, the inhibition of photosynthesis in L658 paralleled the global downregulation of photosynthesis-related genes. Furthermore, the expression of the salicylic acid-related genes non-expressor of pathogenesis related genes 1 (), pathogenesis-related 1 , and pathogenesis-related 5 () was upregulated, while the expression of jasmonic acid- and ethylene-related genes was inhibited in L658 compared with L958. In conclusion, the downregulation of photosynthesis-related genes likely led to a decline in photosynthesis, which may be combined with the inhibition of peroxidase (POD) and catalase (CAT) to generate two stages of HO accumulation. The high level of HO salicylic acid and and in L658 possible initiated the hypersensitive response.
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http://dx.doi.org/10.3390/ijms21165767DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7460852PMC
August 2020

Tripartite motif containing 24 regulates cell proliferation in colorectal cancer through YAP signaling.

Cancer Med 2020 09 17;9(17):6367-6376. Epub 2020 Jul 17.

Department of Pathology, The Seventh Affiliated Hospital of Sun Yat-Sen University, Shenzhen, Guangdong, P.R. China.

The protein, tripartite motif containing 24 (TRIM24) is a member of the TRIM protein family, and acts as a critical co-regulator of multiple nuclear receptors. TRIM24 is dysregulated in many cancers, including colorectal carcinoma. However, its biological functions and molecular mechanisms with respect to colorectal carcinoma are still largely unknown. In the current study, we found that TRIM24 promotes YAP signaling for driving cell proliferation in colorectal cancer. TRIM24 was significantly upregulated in colorectal carcinoma, and its expression was negatively correlated with the survival of patients. Depletion of TRIM24 impaired the ability of the cancer cells to proliferate and form colonies. Furthermore, this study also revealed the mechanism underlying the recruitment of TRIM24 by the DANCR/KAT6A complex, which is bound to acetylated lysine 23 of histone H3 (H3K23), resulting in binding to the YAP promoter and activation of YAP transcription that ultimately enhances the proliferation of colorectal cancer cells. Our results revealed a novel mechanism involving TRIM24-YAP signaling for the regulation of colorectal cancer. We also identified TRIM24 as a potential therapeutic molecule for targeting colorectal cancer.
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http://dx.doi.org/10.1002/cam4.3310DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7476840PMC
September 2020

β-Cyclodextrin coated porous [email protected] nanostructures with enhanced peroxidase-like activity for colorimetric and paper-based determination of glucose.

Mikrochim Acta 2020 07 4;187(8):425. Epub 2020 Jul 4.

Anhui Province Key Laboratory of Advanced Catalytic Materials and Reaction Engineering, School of Chemistry and Chemical Engineering, Hefei University of Technology, Hefei, Anhui, 230009, People's Republic of China.

β-cyclodextrin-functionalized porous [email protected] nanostructures (β[email protected]) with intrinsic and enhanced peroxidase-like activity were successfully synthesized by a two-step method. The synthesized β[email protected] can efficiently catalyze the oxidation of various substrates, such as 3,3',5,5'-tetramethylbenzidine (TMB), mixture of 4-amino antipyrine (4-AAP) and 3,5-dichloro-2-hydroxy acid sodium (DHBS) (4-AAP/DHBS), and mixture of 4-AAP and N-Ethyl-N-(3-sulfopropyl)-3-methyl-aniline sodium salt (TOPS) (4-AAP/TOPS), by HO to generate visual blue, purple, and pink color, respectively. The UV-vis absorbance peak of the three β[email protected] catalyzed the chromogenic reaction system located at 650 nm, 510 nm, and 550 nm, respectively. The β[email protected] TMB-HO chromogenic reaction exhibited higher absorbance intensity, catalytic efficiency, and color stability in comparison to 4-AAP/DHBS-HO and 4-AAP/TOPS-HO chromogenic reactions. The catalytic activity of β[email protected] was enhanced about 4-fold compared to that of [email protected] in terms of K for HO. Using TMB as chromogenic substrate, a colorimetric assay was fabricated for the determination of HO with a detection limit of 2.78 μM (absorbance at 650 nm). The colorimetric determination of glucose with a detection limit of 9.28 μM was further achieved by coupling with glucose oxidase enzymatic reaction, indicating the versatility of the β[email protected] detection strategy. A paper-based detection method coupled with smartphone for fast visual and instrument-free detection of glucose was further developed. Finally, the developed colorimetric assay and paper-based detection method were successfully applied to the determination of glucose in human serum sample. Graphical abstract.
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http://dx.doi.org/10.1007/s00604-020-04410-8DOI Listing
July 2020

Maternal Nicotine Exposure During Gestation and Lactation Period Affects Behavior and Hippocampal Neurogenesis in Mouse Offspring.

Front Pharmacol 2019 22;10:1569. Epub 2020 Jan 22.

Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical University, Xuzhou, China.

Cigarette smoking or nicotine exposure during pregnancy is associated with numerous obstetrical, fetal, and developmental complications, as well as an increased risk of adverse health consequences in the adult offspring. In this study, we examined the effects of maternal nicotine exposure during perinatal and lactation stages on behavioral performance and hippocampal neurogenesis in the adolescent stage of offspring mice. Female C57BL/mice received nicotine in drinking water (200 μg/ml nicotine) or vehicle (1% saccharin) starting from 2 weeks premating until the offspring were weaned on postnatal day 20. Experiments started on postnatal day 35. Female offspring with maternal nicotine exposure presented an increase in anxiety-like behavior in an open-field test. BrdU assay revealed that nicotine offspring presented an increase in cell proliferation in hippocampal dentate gyrus, but the number of BrdU cells was decreased in one week and further decreased in three weeks. The occurrence of disarray of DCX cells increased in both male and female nicotine offspring. The density of microglial marker protein Iba1 was significantly increased in the nicotine offspring. Furthermore, the expression of microglia marker Iba1, the CX3CL1, CX3CR1, and downstream molecules PKA and p-ErK were significantly increased in the nicotine group. In summary, maternal nicotine exposure affects both hippocampal neurogenesis and microglial activity in the adolescent offspring.
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http://dx.doi.org/10.3389/fphar.2019.01569DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6987079PMC
January 2020

Population genetics of swamp eel in the Yangtze River: comparative analyses between mitochondrial and microsatellite data provide novel insights.

PeerJ 2020 21;8:e8415. Epub 2020 Jan 21.

Anhui Key Laboratory of Aquaculture and Stock Enhancement, Fisheries Research Institution, Anhui Academy of Agricultural Sciences, Hefei, China.

The swamp eel () is a typical sex reversal fish with high economic value. Several phylogeographic studies have been performed using various markers but comparative research between mitochondrial and nuclear markers is rare. Here, a fine-scale study was performed across six sites along the Yangtze River including three sites on the main stem and three sites from tributaries. A total of 180 swamp eel individuals were collected. Genetic structure and demographic history were explored using data from two mitochondrial genes and eight microsatellite loci. The results revealed the samples from tributary sites formed three separate clades which contained site-specific lineages. Geographic isolation and the habitat patchiness caused by seasonal cutoff were inferred to be the reasons for this differentiation. Strong gene flow was detected among the sites along the main stem. Rapid flow of the river main stem may provide the dynamic for the migration of swamp eel. Interestingly, the comparative analyses between the two marker types was discordant. Mitochondrial results suggested samples from three tributary sites were highly differentiated. However, microsatellite analyses indicated the tributary samples were moderately differentiated. We conclude this discordance is mainly caused by the unique life history of sex reversal fish. Our study provides novel insights regarding the population genetics of sex reversal fish.
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http://dx.doi.org/10.7717/peerj.8415DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6979408PMC
January 2020

Behavior and Hippocampal Epac Signaling to Nicotine CPP in Mice.

Transl Neurosci 2019 2;10:254-259. Epub 2019 Oct 2.

Key Laboratory of Industrial dust deep reduction and occupational health and safety of Anhui Higher Education Institutes, Anhui University of Science and Technology, Huainan 232001, China.

Tobacco use is a major challenge to public health in the United States and across the world. Many studies have demonstrated that adult men and women differ in their responses to tobacco smoking, however neurobiological studies about the effect of smoking on males and females were limited. Exchange protein directly activated by cAMP (Epac) signaling participates in drug addictive behaviors. In this study, we examined the hippocampal Epac signaling in nicotine-induced place conditioning mice. Nicotine at 0.2 mg/kg and 0.4 mg/kg induced a conditioned place preference (CPP) in male and female mice, respectively. After CPP, male mice presented less anxiety-like behavior as demonstrated by an open-field test. The hippocampal Epac2 protein was elevated in both male and female nicotine place conditioning mice. However, Rap1 protein was elevated and CREB phosphorylation was reduced in female nicotine place conditioning mice. Our data provide direct evidence that hippocampal Epac signaling is altered in nicotine-induced CPP mice. Pharmacology manipulation Epac signaling may open a new avenue for the treatment of nicotine abuse and dependence.
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http://dx.doi.org/10.1515/tnsci-2019-0041DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6778398PMC
October 2019

Multiplexed chemiluminescence determination of three acute myocardial infarction biomarkers based on microfluidic paper-based immunodevice dual amplified by multifunctionalized gold nanoparticles.

Talanta 2020 Jan 10;207:120346. Epub 2019 Sep 10.

CAS Key Laboratory of Soft Matter Chemistry, Collaborative Innovation Center of Chemistry for Energy Materials, Department of Chemistry, University of Science and Technology of China, Hefei, Anhui, 230026, China. Electronic address:

Acute myocardial infarction (AMI) causes significant mortality and morbidity. The determination of multiple AMI biomarkers is very important for the timely diagnosis of AMI. In this work, simultaneous determination of three AMI biomarkers were achieved by virtue of a three-dimensional (3D) microfluidic paper-analytical device (μPAD) with temporally resolved chemiluminescence (CL) emissions for the first time. A dual-signal amplification strategy was introduced including by employing primary antibody functionalized gold nanoparticles (Ab-GNPs) immobilized on the detection zone as amplified capture probes, and Co(II) catalyst, secondary antibody, luminol multifunctionalized gold nanoparticles (Co(II)-Ab-luminol-GNPs) with excellent CL activity as amplified signal probes. CL immunoreactions were performed at three detection zone of the fabricated 3D μPAD by assembling Ab-GNPs, antigen, and Co(II)-Ab-luminol-GNPs to form sandwich-type immunocomplexes. Auto separated CL signals with temporal resolution were obtained by time delayed transport of HO to different detection zones for multiplexed analysis. The CL signal obtained by using Co(II)-Ab-luminol-GNPs as signal probe (10576 a.u.) were about 20-fold higher than that by using conventional horseradish peroxidase labeled antibody modified luminol-GNPs as signal probe (531 a.u.). Finally, three AMI biomarkers including heart-type fatty acid-binding protein (H-FABP), cardiac troponin I (cTnI) and copeptin were quantitatively analyzed in one CL detection run by reading the CL intensity of the obtained three CL emission peaks. The detection range were ultra-wide ranged from 0.1 pg/mL to 1 μg/mL, 0.5 pg/mL to 1 μg/mL and 1 pg/mL to 1 mg/mL with the detection limits down to 0.06 pg/mL, 0.3 pg/mL and 0.4 pg/mL for H-FABP, cTnI and copeptin detection, respectively. The developed μPAD based immunoassay performing multiplexed analysis ability, high sensitivity, ultra-wide dynamic range, favorable selectivity, accessible accuracy and reproducibility, have great application potential for the early diagnosis of AMI.
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http://dx.doi.org/10.1016/j.talanta.2019.120346DOI Listing
January 2020

The complete mitochondrial genome of (Cyprinidae, Barbinae) from Xinanjiang River.

Mitochondrial DNA B Resour 2019 Sep 23;4(2):3159-3160. Epub 2019 Sep 23.

Fisheries Institute, Anhui Academy of Agricultural Sciences, Hefei, China.

The complete mt genome sequence of was obtained by PCR, containing 37 genes with 13 protein coding genes, 22 transfer RNAs (tRNAs), two ribosomal RNAs (rRNAs) and a non-coding control region.
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http://dx.doi.org/10.1080/23802359.2019.1667916DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7706916PMC
September 2019

Exploration on the bioreduction mechanism of Cr(Ⅵ) by a gram-positive bacterium: Pseudochrobactrum saccharolyticum W1.

Ecotoxicol Environ Saf 2019 Nov 16;184:109636. Epub 2019 Sep 16.

School of Minerals Processing and Bioengineering, Key Laboratory of Biohydrometallurgy of Ministry of Education, Central South University, Changsha, 410083, China. Electronic address:

Bioremediation of chromium (Cr(Ⅵ)) contaminations has been widely reported, but the research on its removal mechanism is still scarce. Studies on Cr(Ⅵ) removal by strains affiliated to genus Pseudochobactrum revealed the Cr(Ⅵ) efficiency removal through the reduction of Cr(Ⅵ) to Cr(Ⅲ). However, the location of Cr(Ⅵ) reduction reaction and exact mechanism are still unspecified. In this work, a Gram-positive bacterial strain, Pseudochrobactrum saccharolyticum W1 (P. saccharolyticum W1) was isolated and tested to remove approximately 53.7% of Cr(Ⅵ) (initial concentration was 200 mg L) from the MSM medium. Analysis of SEM-EDS and TEM-EDS indicated that chromium-containing particles precipitated both on the cell surface and in the cytoplasm. Batch experiments indicated that the heat-treated bacterial cells almost had no ability to remove Cr(Ⅵ) from solution, while the resting cells could remove 62.0% of Cr(Ⅵ) at the initial concentration of 10 mg L. Additionally, at this concentration, 64.8% and 70.8% of Cr(Ⅵ) was reduced by cell envelope components and intracellular soluble substances after 6 h, respectively. These results suggested that the removal of Cr(Ⅵ) by P. saccharolyticum W1 was through direct reduction, which occurred on both cell envelop and cytoplasm. The results also showed that cytoplasm was the main site for Cr(Ⅵ) reduction compared to the cell envelop. Further analysis of FTIR and XPS verified that C-H, C-C, CO, C-OH and C-O-C groups of cells involved in correlation with chromium during Cr(Ⅵ) reduction. The study offered an insight into the Cr(VI) reduction mechanism of P. saccharolyticum W1.
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http://dx.doi.org/10.1016/j.ecoenv.2019.109636DOI Listing
November 2019

Genomic deletion of TLR2 induces aggravated white matter damage and deteriorated neurobehavioral functions in mouse models of Alzheimer's disease.

Aging (Albany NY) 2019 09 11;11(17):7257-7273. Epub 2019 Sep 11.

Department of Neurology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China.

Toll-like receptor-2 (TLR2), a member of the TLR family, plays an important role in the initiation and regulation of immune/inflammation response, which is a critical mechanism underlying Alzheimer's disease (AD). To clarify the role of TLR2 in the pathological process of AD, in the present study, TLR2 knockout plus APPswe/PSEN1dE9 transgenic mice (AD-TLR2KO) were generated. Neurobehavioral tests and brain MRI scan were conducted on mice at the age of 12 months. Additionally, neuron loss was evaluated using NeuN staining. Amyloid β protein (Aβ), glial fibrillary acidic protein (GFAP), endogenous ligands for TLR2, and the activation of downstream signaling of TLR2 in mouse brains were detected by immunohistochemistry and Western blots. The results demonstrated that TLR2 deficit induced learning disabilities, decreased spontaneous activity, increased anxiety and depression, and led to white matter damage (WMD), brain atrophy, loss of neurons, and glial activation. Moreover, TLR2 deficit aggravated impaired neurobehavioral functions and WMD in AD mice, but did not affect the Aβ deposition in mouse brains. Our data indicate that the genomic deletion of TLR2 impairs neurobehavioral functions, induces WMD and brain atrophy, and increases the activation of astrocytes, which in turn aggravate the symptoms of AD through a non-Aβ mechanism.
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http://dx.doi.org/10.18632/aging.102260DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6756907PMC
September 2019

Effect of Autophagy Regulated by Sirt1/FoxO1 Pathway on the Release of Factors Promoting Thrombosis from Vascular Endothelial Cells.

Int J Mol Sci 2019 Aug 24;20(17). Epub 2019 Aug 24.

Department of Pharmacology, China Pharmaceutical University, Nanjing 210009, China.

Factors promoting thrombosis such as von Willebrand factor (vWF) and P-selectin are essential for the development of atherosclerosis (AS) and arterial thrombosis. The processing, maturation and release of vWF are regulated by autophagy of vascular endothelial cells. The Sirt1/FoxO1 pathway is an important pathway to regulate autophagy of endothelial cells, therefore the Sirt1/FoxO1 pathway may be an important target for the prevention of thrombosis. We investigated the role of ox-LDL in the release of vWF and P-selectin and the expression of Sirt1 and FoxO1 by Western Blot, Flow Cytometry, ELISA, and tandem fluorescent mRFP-GFP-LC3. We found that vWF and P-selectin secretion increased and Sirt1/FoxO1 pathway was depressed in human umbilical vein endothelial cells (HUVEC) when treated with ox-LDL. Moreover, the expression of autophagy-related protein LC3-II/I and p62 increased. Then, we explored the relationship between autophagy regulated by the Sirt1/FoxO1 pathway and the secretion of vWF and P-selectin. We found that Sirt1/FoxO1, activated by the Sirt1 activators resveratrol (RSV) and SRT1720, decreased the secretion of vWF and P-selectin, which can be abolished by the autophagy inhibitor 3-MA. The expression of Rab7 increased when Sirt1/FoxO1 pathway was activated, and the accumulation of p62 was decreased. Autophagy flux was inhibited by ox-LDL and Sirt1/FoxO1 pathway might enhance autophagy flux through the promotion of the Rab7 expression. Taken together, our data suggest that by enhancing autophagy flux and decreasing the release of vWF and P-selectin, the Sirt1/FoxO1 pathway may be a promising target to prevent AS and arterial thrombosis.
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http://dx.doi.org/10.3390/ijms20174132DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6747322PMC
August 2019

Three-dimensional microfluidic paper-based device for multiplexed colorimetric detection of six metal ions combined with use of a smartphone.

Anal Bioanal Chem 2019 Sep 27;411(24):6497-6508. Epub 2019 Jul 27.

Anhui Province Key Laboratory of Advanced Catalytic Materials and Reaction Engineering, School of Chemistry and Chemical Engineering, Hefei University of Technology, Hefei, 230009, Anhui, China.

A simple double-layered three-dimensional (3D) microfluidic paper-based analytical device (μPAD) was designed for the simultaneous determination of six metal ions-Fe(III), Ni(II), Cr(VI), Cu(II), Al(III), and Zn(II)-for the first time. The 3D μPAD was composed of two paper layers: a top pretreatment layer and a bottom colorimetric detection layer. The sample solution added to the central sample reservoir of the 3D μPAD could be automatically divided into eight flow pathways and be automatically pretreated while flowing through the pretreatment zones located in the microfluidic channels, and automatically carried out the chromogenic reactions after reaching the detection zones. Random diffusion of the chromogenic reagents was effectively prevented by transport of the pretreated sample solution to the detection zones through 3D microfluidic channels with an L-type circuitous flow route design, resulting in highly increased color uniformity and reproducibility. Combined with use of a flat LED lamp as an upward lighting source and a smartphone as a convenient detector, improved color perception, highly enhanced sensitivity, and an extended detection range were obtained. Finally, the double-layered 3D μPAD was applied to the multiplexed determination of the six metal ions in mixtures and environmental samples with satisfactory results. Detection limits as low as 0.2, 0.3, 0.1, 0.03, 0.08, and 0.04 mg/L for Fe(III), Ni(II), Cr(VI), Cu(II), Al(III), and Zn(II) detection, respectively, were achieved, which are about one order of magnitude lower than obtained with previously reported μPADs for the detection of metal ions. The present 3D μPAD is simple, fast, selective, sensitive, and user-friendly, and holds great application potential for multiplexed on-site analysis.
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http://dx.doi.org/10.1007/s00216-019-02032-5DOI Listing
September 2019

Corrosion Acceleration of Printed Circuit Boards With an Immersion Silver Layer Exposed to in an Aerobic Medium.

Front Microbiol 2019 2;10:1493. Epub 2019 Jul 2.

Corrosion and Protection Center, University of Science and Technology Beijing, Beijing, China.

In this research, the corrosion behavior of printed circuit boards with an immersion silver layer (PCB-ImAg) exposed to bacteria in Luria-Bertani broth was investigated. The growth test demonstrated that had a high copper tolerance. Analysis of surface and cross-sectional view of the samples after immersion test indicated that metabolites produced by accelerated the microporous corrosion of PCB-ImAg, and the biofilm that adhered to the surface led to oxygen concentration corrosion. Electrochemical impedance spectroscopy tests confirmed that the microbiologically influenced corrosion of PCB-ImAg was related to the biofilm formation and metabolism.
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http://dx.doi.org/10.3389/fmicb.2019.01493DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6614184PMC
July 2019

Effect of Dissolved Oxygen Concentration on the Microbiologically Influenced Corrosion of Q235 Carbon Steel by Halophilic Archaeon .

Front Microbiol 2019 25;10:844. Epub 2019 Apr 25.

Beijing Advanced Innovation Center for Materials Genome Engineering, Institute for Advanced Materials and Technology, University of Science and Technology Beijing, Beijing, China.

The influence of dissolved oxygen concentration (DOC) on the microbiologically influenced corrosion (MIC) of Q235 carbon steel in the culture medium of halophilic archaeon was investigated. The increase of DOC from 0.0 to 3.0 ppm was found to strengthen the oxygen concentration cell by promoting cathodic reaction. Meanwhile, the increased DOC also promoted archaeal cell growth, which could consume more metallic iron as energy source and aggravated the localized corrosion. When the DOC further increased to 5.0 ppm, the uniform corrosion was dominant as the biofilms became uniformly presented on the steel surface. Combined with the stronger inhibition effect of oxygen diffusion by the increased biofilm coverage, the MIC of carbon steel in the 5.0 ppm medium was weaker than that in the 3.0 ppm medium. From weight loss and electrochemical tests, the results all demonstrated that the carbon steel in the 3.0 ppm medium had the largest corrosion rate.
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http://dx.doi.org/10.3389/fmicb.2019.00844DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6495067PMC
April 2019

Elevated pulmonary tuberculosis biomarker miR-423-5p plays critical role in the occurrence of active TB by inhibiting autophagosome-lysosome fusion.

Emerg Microbes Infect 2019 ;8(1):448-460

a Institute of Cell Biology , Zhejiang University School of Medicine , Hangzhou , People's Republic of China.

Rapid diagnosis of pulmonary tuberculosis is an effective measure to prevent the spread of tuberculosis. However, the grim fact is that the new, rapid, and safe methods for clinical diagnosis are lacking. Moreover, although auto-lysosome is critical in clearing Mycobacterium tuberculosis, the pathological significance of microRNAs, as biomarkers of tuberculosis, in autophagosome maturation is unclear. Here, these microRNAs were investigated by Solexa sequencing and qPCR validation, and a potential diagnostic model was established by logistic regression. Besides that, the mechanism of one of the microRNAs involved in the occurrence of tuberculosis was studied. The results showed that the expression of miR-423-5p, miR-17-5p, and miR-20b-5p were significantly increased in the serum of patients with tuberculosis. The combination of these three microRNAs established a model to diagnose tuberculosis with an accuracy of 78.18%, and an area under the curve value of 0.908. Bioinformatics analysis unveiled miR-423-5p as the most likely candidate in regulating autophagosome maturation. The up-regulation of miR-423-5p could inhibit autophagosome maturation through suppressing autophagosome-lysosome fusion in macrophages. Further investigations showed that VPS33A was the direct target of miR-423-5p, and the two CUGCCCCUC domains in VPS33A 3'-UTR were the direct regulatory sites for miR-423-5p. In addition, an inverse correlation between VPS33A and miR-423-5p was found in peripheral blood mononuclear cells of patients with tuberculosis. Since the inhibition of autolysosome formation plays a critical role in tuberculosis occurrence, our findings suggests that miR-423-5p could suppress autophagosome-lysosome fusion by post-transcriptional regulation of VPS33A, which might be important for the occurrence of active tuberculosis.
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http://dx.doi.org/10.1080/22221751.2019.1590129DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6455132PMC
August 2019

Comparative Proteomic Analysis of Wheat Carrying Response to f. sp. Using Two-Dimensional Electrophoresis.

Int J Mol Sci 2019 Feb 21;20(4). Epub 2019 Feb 21.

College of Agronomy & Key Laboratory for Major Crop Diseases, Sichuan Agricultural University, Chengdu 611130, China.

Wheat powdery mildew caused by f. sp. () is considered a major wheat leaf disease in the main wheat producing regions of the world. Although many resistant wheat cultivars to this disease have been developed, little is known about their resistance mechanisms. is a broad, effective resistance gene against powdery mildew in wheat line . The aim of this study was to investigate the resistance proteins after inoculation in wheat lines , , and . with was used as the resistant control, and without any effective genes was the susceptible control. Proteins were extracted from wheat leaves sampled 2, 4, 8, 12, and 24 h after inoculation, separated by two-dimensional electrophoresis, and stained with Coomassie brilliant blue G-250. The results showed that different proteins were upregulated and downregulated in three wheat cultivars at different time points. For the wheat cultivar , a total of 62 proteins were upregulated and 71 proteins were downregulated after inoculation. Among these, 46 upregulated proteins were identified by mass spectrometry analysis using the NCBI nr database of . The identified proteins were predicted to be associated with the defense response, photosynthesis, signal transduction, carbohydrate metabolism, energy pathway, protein turnover, and cell structure functions. It is inferred that the proteins are not only involved in defense response, but also other physiological and cellular processes to confer wheat resistance against . Therefore, the resistance products potentially mediate the immune response and coordinate other physiological and cellular processes during the resistance response to . The lipoxygenase, glucan exohydrolase, glucose adenylyltransferasesmall, phosphoribulokinase, and phosphoglucomutase are first reported to be involved in the interactions of wheat- at early stage. The further study of these proteins will deepen our understanding of their detailed functions and potentially develop more efficient disease control strategies.
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http://dx.doi.org/10.3390/ijms20040933DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6412634PMC
February 2019

The effects of negative context and attachment security priming on working memory updating among anxiously attached individuals.

Biol Psychol 2019 04 14;143:41-52. Epub 2019 Feb 14.

Southwest University, Chongqing, 400715, China.

Two studies using event-related potentials ERPs) combined with emotional versions of 2-back tasks were performed to examine the effects of negative context on working memory (WM) updating task performance among anxiously attached individuals. One study also assessed the soothing effect of priming memories of attachment security on task performance. Three types of information, including negative attachment pictures, general negative pictures and neutral pictures, were used as materials in the present study. Impairment in WM updating capacity was found in the context of negative attachment pictures in both studies, and the ERP results revealed the following dynamic process: the participants showed enhanced attention to negative attachment stimuli during the initial encoding stage, as expressed by a larger P1, but undue immersion in negative emotion led to a reduced P300 during the elaborate stage. However, security priming was useful in reducing mood disturbance in the context of a WM updating task, and the participants performed better on the task after secure attachment activation. The implications of these findings for emotional WM updating capacity and information processing patterns among anxiously attached individuals are discussed.
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http://dx.doi.org/10.1016/j.biopsycho.2019.02.006DOI Listing
April 2019

Genetic deletion of β adrenergic receptors exacerbates hepatocellular lipid accumulation in high-fat diet mice.

Biochem Biophys Res Commun 2019 03 13;511(1):73-78. Epub 2019 Feb 13.

Department of Pharmacology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China; The Key Laboratory for Drug Target Research and Pharmacodynamic Evaluation of Hubei Province, Wuhan, 430030, China. Electronic address:

β Adrenergic receptors (βARs) are G protein-coupled receptors (GPCRs) that are expressed in major insulin target tissues. βARs play an important role in the regulation of lipid metabolism during aging; however, little is known about the significance of βARs in the pathogenesis of hepatic fat accumulation in high-fat diet (HFD) mice. This study aims to examine the role of βAR in the development of nonalcoholic fatty liver disease (NAFLD) induced by HFD and the underlying mechanisms. Surprisingly, we found that genetic deletion of βAR significantly increased the liver weight of mice fed a HFD for 20 weeks compared to that of wild-type (WT) mice. Moreover, genetic deletion of βAR could aggravate HFD-induced liver lipid accumulation and liver injury in mice. Mechanistically, we demonstrated that βAR deletion significantly activated PPARγ/CD36 signaling via inactivation of the cAMP response element-binding (CREB) protein to facilitate hepatocellular lipid deposition in HFD mice. Together, our results identify βAR as a plausible therapeutic target for preventing or treating NAFLD.
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http://dx.doi.org/10.1016/j.bbrc.2019.02.037DOI Listing
March 2019