Publications by authors named "Yuri Seo"

23 Publications

  • Page 1 of 1

World Health Organization Integrated Care for Older People (ICOPE) and the Integrated Care of Older Patients with Frailty in Primary Care (ICOOP_Frail) Study in Korea.

Ann Geriatr Med Res 2021 03 30;25(1):10-16. Epub 2021 Mar 30.

National Evidence-based Healthcare Collaborating Agency, Seoul, Korea.

The World Health Organization (WHO) recently endorsed the proposal for a Decade of Healthy Ageing (2020-2030). The WHO defines "healthy aging" as "the process of developing and maintaining the functional ability that enables wellbeing in older age." Among the strategies for the Decade of Healthy Ageing, the WHO has suggested enhancing intrinsic capacity, promoting functional ability, and implementing the Integrated Care for Older People (ICOPE) package. The WHO has defined steps for ICOPE evaluation and scale-up and is performing a prospective study in 2-3 countries (low and middle income, high income) to test its feasibility in 2021-2022 and a multinational randomized study to validate its clinical efficacy and effectiveness in 2022-2024. Intrinsic capacity and frailty represent two faces of the same coin, with one indicating the reserves of the individual and the other indicating the deficits that accumulate with age. The Integrated Care of Older Patients with Frailty in Primary Care (ICOOP_Frail) study is the first integrated care program for frailty or functional decline in primary care in Korea. The results suggest that the ICOOP_Frail study can be utilized as a reference for ICOPE studies in Korea or at least to provide important findings for the forthcoming ICOPE implementation study in Korea.
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http://dx.doi.org/10.4235/agmr.21.0025DOI Listing
March 2021

Gene expression profiles of pro-inflammatory mediators in the conjunctiva of patients with epiblepharon.

Graefes Arch Clin Exp Ophthalmol 2021 Feb 5. Epub 2021 Feb 5.

Department of Ophthalmology, Yongin Severance Hospital, The Institute of Vision Research, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 03722, Republic of Korea.

Purpose: To investigate the gene expression of pro-inflammatory mediators in the conjunctiva of pediatric patients with epiblepharon in a case-control study.

Methods: Twenty healthy controls and 15 pediatric patients with epiblepharon were enrolled from April 23, 2020 to June 15, 2020. Epiblepharon severity was divided into class I-III (least to moderate severity) and class IV (most severe). We obtained impression cytologic specimens from the medial palpebral conjunctiva of the participants to measure the gene expression of interleukin (IL)-1β, IL-6, matrix metalloproteinase 9 (MMP9), and mucin 5AC (MUC5AC) using quantitative reverse transcription polymerase chain reaction.

Results: The mean age in the epiblepharon group was 9 years (range 7.5-11 years), and that in the healthy control group was 9.5 years (range 8-11.3 years). IL-1β, IL-6, and MMP9 expression levels were 2.08 (p < 0.05), 2.11 (p < 0.05), and 2.48 (p < 0.05) fold higher, respectively, in the epiblepharon group than in the healthy control group. However, MUC5AC gene expression was not different between healthy subjects and patients with epiblepharon. IL-1β, IL-6, and MMP9 expression levels in class IV patients were 1.32 (p < 0.05), 1.77 (p < 0.05), and 1.98 (p < 0.05) fold higher, respectively, than in class I-III patients.

Conclusion: Epiblepharon may induce chronic inflammatory changes in the conjunctiva in addition to corneal epithelial damage. Therefore, early corrective surgery should be considered to prevent conjunctival inflammation.
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http://dx.doi.org/10.1007/s00417-021-05089-0DOI Listing
February 2021

Risk Factors Influencing the Occurrence and Severity of Symptomatic Dry Eye Syndrome: A Cross-sectional Study.

Ophthalmic Epidemiol 2021 Jan 31:1-7. Epub 2021 Jan 31.

Department of Preventive Medicine, Yonsei University College of Medicine , Seoul, Republic of Korea.

: We aimed to investigate the prevalence and risk factors of dry eye syndrome (DES) among a population-based cohort study.: This cross-sectional study was conducted on 475 subjects (184 men and 291 women) enrolled in the Study Group for Environmental Eye Disease at July 2013. Using the ocular surface disease index (OSDI), we measured the DES severity and defined DES as OSDI score ≥13. Current symptoms of DES and possible risk factors such as body mass index, occupations, comorbidities, exercise, smoking and drinking status were assessed by multivariate logistic regression.: Prevalence of DES was significantly higher in women (52.6%) than in men (41.9%) ( < .001). Compared to white-collar workers, blue-collar workers and unemployed persons showed significantly higher DES prevalence and severity. Compared to those with low BMI (<23.0 kg/m), people with extremely high BMI (≥30.0 kg/m) had significantly higher odds ratio (OR) of having DES after fully adjusted for sex, age, hypertension, diabetes, menopausal status, hormone replacement therapy, occupation, and lifestyle factors (OR: 2.83, 95% confidence interval: 1.04-7.71).: We found some novel factors which have been unknown to the relationship with DES through the five years observation of the cohort. The positive associations of unemployment status, blue-collar work, alcohol habit, and obesity with DES suggests a person's comprehensive condition, not individual factors, contribute significantly in developing DES. Further studies will be helpful to understand the underlying mechanisms.
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http://dx.doi.org/10.1080/09286586.2021.1879172DOI Listing
January 2021

Differences in the Association of Neighborhood Environment With Physical Frailty Between Urban and Rural Older Adults: The Korean Frailty and Aging Cohort Study (KFACS).

J Am Med Dir Assoc 2021 Mar 18;22(3):590-597.e1. Epub 2020 Nov 18.

Department of Family Medicine, College of Medicine, Kyung Hee University, Seoul, Republic of Korea. Electronic address:

Objective: We examined the difference in the relationship between perceived neighborhood environments and physical frailty between urban and rural older adults.

Design: Cross-sectional study.

Setting And Participants: Urban and rural community in South Korea; a total of 2593 community-dwelling older adults aged 70-84 years (mean age: 76.0 years, 51.0% women) in the Korean Frailty and Aging Cohort Study (KFACS).

Methods: The neighborhood environment was assessed using the 17-item Environmental Module of the International Physical Activity Questionnaire (IPAQ-E). The IPAQ-E had 7 environmental factors (residential density, access to destinations, neighborhood infrastructure, neighborhood safety, social environment, aesthetic qualities, and street connectivity). Physical frailty was defined using the Fried frailty criteria with modified cutoffs.

Results: The number of participants residing in urban and rural areas was 1902 and 691, respectively. The prevalence of frailty was 5.3% and 12.0% in urban and rural areas, respectively. In urban areas, frailty was associated with the total IPAQ-E score (β = -0.007, P = .009) after adjusting for confounding factors. There was no association in the rural areas (β = -0.003, P = .535). In urban older adults, logistic regression showed that absence of destination [odds ratio (OR) 2.58, 95% confidence interval (CI) 1.36-4.90] and no crime safety at night (OR 2.00, 95% CI 1.12-3.57) were associated with a higher risk of frailty. In rural older adults, poor access to recreational facilities (OR 2.17, 95% CI 1.07-4.40) and no aesthetics (OR 2.49, 95% CI 1.22-5.10) were associated with frailty.

Conclusions And Implications: Our study indicated that the presence of destination and crime safety at night was significantly associated with physical frailty among older adults living in urban areas, whereas aesthetics and recreational facilities were significantly associated with physical frailty in rural older adults.
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http://dx.doi.org/10.1016/j.jamda.2020.09.044DOI Listing
March 2021

Maternal nutrition intervention focused on the adjustment of salt and sugar intake can improve pregnancy outcomes.

Food Sci Nutr 2020 Jul 10;8(7):3900-3911. Epub 2020 Jun 10.

Department of Medical Nutrition Graduate School of East-West Medical Science Kyung Hee University Yongin Korea.

Due to the increasing age of pregnant women, maternal nutrition management is becoming more important. Since pregnant women are more likely to consume sodium and sugars than nonpregnant women of the same age, we investigated whether maternal nutrition intervention focused on the adjustment of salt and sugar intake can help pregnancy outcome. This randomized controlled trial was performed on 142 pregnant women within 22 weeks of gestational age for at least 16 weeks until childbirth. Subjects were unequally assigned to the intervention group ( = 98) and the control group ( = 44). Dietary changes based on perceived taste preferences were evaluated by 24-hr dietary recall and food frequency questionnaires (FFQ) at pre- and postintervention. In the intervention group, while the intakes of energy, protein, and vitamins were maintained, the intakes of sodium ( < .001) and sugar from processed food ( < .05) were significantly reduced after the intervention. The decreases in salt and sugar consumption were more pronounced in the mothers who had a high preference for saltiness and sweetness. The mean neonatal birth weight of the intervention group was significantly greater than the weight of control group, (3,251.5 ± 402.2 g vs. 2,974.5 ± 294.8 g, < .05). Through this study, nutrition intervention was found to be effective for the formation of healthy eating habits such as reduced salt and sugar intake in pregnant women especially with a high preference for saltiness and sweetness. Also, such specialized maternal nutrition intervention during pregnancy promotes the birth of healthy newborn babies of normal weight.
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http://dx.doi.org/10.1002/fsn3.1699DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7382176PMC
July 2020

p32/C1QBP regulates OMA1-dependent proteolytic processing of OPA1 to maintain mitochondrial connectivity related to mitochondrial dysfunction and apoptosis.

Sci Rep 2020 06 30;10(1):10618. Epub 2020 Jun 30.

Graduate School of Analytical Science and Technology (GRAST), Chungnam National University, 99 Daehak-ro(St), Yusoeng, Taejon, 305-764, Republic of Korea.

Mitochondria are dynamic organelles that undergo fusion and fission in response to various physiological and stress stimuli, which play key roles in diverse mitochondrial functions such as energy metabolism, intracellular signaling, and apoptosis. OPA1, a mitochondrial dynamin-like GTPase, is responsible for the inner membrane fusion of mitochondria, and the function of OPA1 is regulated by proteolytic cleavage in response to various metabolic stresses. Growing evidences highlighted the importance of mitochondrial adaptation in response to metabolic stimuli. Here, we demonstrated the role of p32/C1QBP in mitochondrial morphology by regulating OMA1-dependent proteolytic processing of OPA1. Genetic ablation of p32/C1QBP activates OMA1, cleaves OPA1, and leads mitochondrial fragmentation and swelling. The loss of p32/C1QBP decreased mitochondrial respiration and lipid utilization, sensitized cells to mitochondrial stress, and triggered a metabolic shift from oxidative phosphorylation to glycolysis, which were correlated with apoptosis in cancer cells and the inhibition of 3D-spheroid formation. These results suggest a unique regulation of cell physiology by mitochondria and provide a basis for a new therapeutic strategy for cancer.
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http://dx.doi.org/10.1038/s41598-020-67457-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7327069PMC
June 2020

Relationship between Alcohol Consumption and Ocular Pressure according to Facial Flushing in Korean Men with Obesity.

Korean J Fam Med 2019 Nov 31;40(6):399-405. Epub 2019 Oct 31.

Department of Family Medicine, Research Institute for Medical Science, Chungnam National University School of Medicine, Daejeon, Korea.

Background: This study aimed to examine the relationship between alcohol consumption and intraocular pressure (IOP) according to facial flushing in Korean men with obesity.

Methods: The study included 479 Korean men with a body mass index of ≥25 kg/m2 (75 non-drinkers, 174 with drinking-related facial flushing, and 230 without facial flushing) who underwent health check-ups between October 1, 2016 and March 31, 2017. Multivariate logistic regression was used to assess the relationship between alcohol consumption and high IOP (≥21 mm Hg).

Results: Flushers consuming ≤16 drinks per week had a significantly higher risk of high IOP than non-drinkers, depending on alcohol consumption (≤8 standard drinks: odds ratio [OR], 4.49; 95% confidence interval [CI], 1.05- 19.25; >8 but ≤16 standard drinks: OR, 8.14; 95% CI, 1.37-48.45). However, when the consumption was >16 drinks per week, the high IOP risk did not significantly increase (OR, 0.71; 95% CI, 0.05-10.69). In addition, there was no significant relationship between alcohol consumption and high IOP among non-flushers consuming ≤8 drinks per week (OR, 2.07; 95% CI, 0.52-8.19). However, a significantly increased risk of high IOP was observed among nonflushers consuming >8 drinks per week, depending on alcohol consumption (>8 but ≤16 standard drinks: OR, 4.84; 95% CI, 1.14-20.61; >16 standard drinks: OR, 4.08; 95% CI, 1.02-16.26).

Conclusion: This study suggests that obese men with alcohol flush reactions may have an increased risk of high IOP with the consumption of smaller amounts of alcohol than non-flushers.
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http://dx.doi.org/10.4082/kjfm.18.0131DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6887763PMC
November 2019

Effects of Orbital Decompression on Lamina Cribrosa Depth in Patients with Graves' Orbitopathy.

Korean J Ophthalmol 2019 Oct;33(5):436-445

Department of Ophthalmology, Institute of Vision Research, Severance Hospital, Yonsei University College of Medicine, Seoul, Korea.

Purpose: We sought to investigate the effects of Graves' orbitopathy (GO) and orbital decompression on lamina cribrosa depth (LCD) using spectral-domain optical coherence tomography.

Methods: Forty eyes that underwent orbital decompression to relieve compressive optic neuropathy or correct disfiguring exophthalmos in the context of GO were included. Subjects were imaged with spectral-domain optical coherence tomography before surgery and at 1 and 3 months after surgery, at which the examiner measured the LCD (distance from the anterior surface of the lamina cribrosa to the Bruch membrane opening line) and peripapillary retinal nerve fiber layer thickness. Subjects were divided into two groups-a muscle-dominant group composed of patients who had extraocular muscle enlargement on preoperative orbital computed tomography scan and a fat-dominant group composed of patients who did not show extraocular muscle enlargement on preoperative orbital computed tomography scan-and subgroup analysis was performed. Preoperative and postoperative intraocular pressure, exophthalmos, LCD, and retinal nerve fiber layer thickness were evaluated.

Results: At baseline, LCD was remarkably shallower in the muscle-dominant group than in the fat-dominant group (95% confidence interval, = 0.007). In the muscle-dominant group, LCD showed no definite change after surgery. However, the fat-dominant group showed temporary posterior displacement of the lamina cribrosa at 1-month postoperation that was reversed to baseline at 3 months postoperation (95% confidence interval, < 0.01).

Conclusions: The lamina cribrosa was anteriorly displaced preoperatively, and its position was nearly unchanged after the surgery, especially in association with extraocular muscle enlargement. An enlarged extraocular muscle could reduce the pressure-relieving effect of orbital decompression around the scleral canal in patients with GO.
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http://dx.doi.org/10.3341/kjo.2019.0036DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6791952PMC
October 2019

Comparison of Progression to Advanced Stage between Polypoidal Choroidal Vasculopathy and Age-Related Macular Degeneration in Korea.

Ophthalmol Retina 2018 05 31;2(5):475-480. Epub 2017 Oct 31.

Institute of Vision Research, Department of Ophthalmology, Yonsei University College of Medicine, Seoul, Korea. Electronic address:

Purpose: To compare the rate of progression to advanced stage in the fellow eye of patients with typical age-related macular degeneration (AMD) and polypoidal choroidal vasculopathy (PCV) in a South Korean cohort.

Design: This is an observational, consecutive retrospective case series.

Participants: Patients with unilateral advanced stage AMD (n = 288; 180 typical AMD patients and 108 PCV patients).

Methods: Clinical assessment included detailed eye examination, including fundus photography, fluorescein angiography, and indocyanine green angiography.

Main Outcome Measures: Five-year progression rate to advanced stage in the fellow eye based on initial Age-Related Eye Disease Study (AREDS) score and the correlation between the initial AREDS score and progression to advanced disease in the fellow eye according to types of AMD.

Results: Five-year progression to advanced disease in the fellow eye was similar between typical AMD and PCV cases (11.1% vs. 14.8 %, respectively; P = 0.466, log-rank test). Among patients with initial AREDS score of 2 (normal macula or small drusen on the fellow eye), a higher proportion of patients progressed to advanced disease in the PCV group compared with typical AMD patients (10.4% vs. 2.4 %, respectively; P = 0.0042, log-rank test). Initial AREDS score correlated significantly with progression of the fellow eye to advanced stage in the typical AMD group, after adjusting for age, gender, and other comorbidities (hazard ratio [HR], 9.5; 95% confidence interval [CI], 2.80-32.12; P = 0.0003). However in the PCV group, initial AREDS score did not correlate with progression to advanced stage in the fellow eye (HR, 1.84; 95% CI, 0.83-4.05; P = 0.13).

Conclusions: Unlike typical AMD, PCV progresses without typical features such as drusen or pigmentary abnormality. Baseline AREDS score was less likely to predict progression of the fellow eye to advanced-stage disease in PCV compared with typical AMD. Therefore, the globally recognized risk-scoring AREDS system may not be applicable in Asia, where PCV is a prevalent subtype of AMD.
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http://dx.doi.org/10.1016/j.oret.2017.08.021DOI Listing
May 2018

A comparative study of dietary habits and nutritional intakes among Korean adults according to current depression status.

Authors:
Yuri Seo Youjin Je

Asia Pac Psychiatry 2018 Sep 21;10(3):e12321. Epub 2018 May 21.

Department of Food and Nutrition, Kyung Hee University, Seoul, South Korea.

Introduction: Data regarding the association between depression and dietary factors in Korean adults are limited. We hypothesized that Korean men and women with depression would have poorer nutritional intakes and different dietary habits than those without depression.

Methods: This study was based on data from the Korea National Health and Nutrition Examination Survey conducted during 2012 to 2014. Overall, 10 591 subjects (4308 men, 6529 women) aged 19 to 64 years were selected and categorized according to current depression status. Food and nutrient intakes were estimated using semi-quantitative food frequency questionnaires.

Results: Compared with non-depression, depression was associated with a higher body mass index and likelihood of a separated/divorced/widowed status, in addition to lower physical activity and employment rates. After adjusting for multiple variables, men with depression had lower intakes of rice cakes (P = 0.017) and eggs (P = 0.039) and a higher intake of chocolate (P = 0.040) than their non-depressed counterparts. Women with depression had a higher intake of carbonated drinks than those without depression (P = 0.015). However, the groups did not differ significantly in terms of nutrient intakes. A multivariable logistic regression analysis of dietary habits showed that depression correlated inversely with eating out (≥1 time/day vs <1 time/month) among men [odds ratio (OR) = 0.23, 95% confidence interval (CI): 0.10-0.55] and women (OR = 0.14, 95% CI: 0.05-0.35) and positively with a lower number of daily meals (<3 vs ≥3) among women (OR = 1.45, 95% CI: 1.01-2.11).

Discussion: Current depression status was associated with differences in some specific dietary intakes and habits in a sample of Korean adults.
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http://dx.doi.org/10.1111/appy.12321DOI Listing
September 2018

Use of fludrocortisone for intradialytic hypotension.

Kidney Res Clin Pract 2018 Mar 31;37(1):85-88. Epub 2018 Mar 31.

Department of Nephrology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.

Intradialytic hypotension during dialysis adversely affects a patient's prognosis and increases mortality. We report a case in which intradialytic hypotension that persisted after the administration of midodrine was relieved after the use of fludrocortisone. Administration of 0.2 mg of fludrocortisone occurred 30 minutes before dialysis. We compared 45 sessions of dialysis without fludrocortisone administration and 45 sessions of dialysis with fludrocortisone administration in one patient. The number of times in which systolic blood pressure became lower than 80 mmHg and the number of early terminations of dialysis due to a decrease in systolic blood pressure were higher in the sessions without fludrocortisone administration than in the sessions with fludrocortisone administration ( < 0.05). Fludrocortisone may be helpful for the treatment of intradialytic hypotension that does not respond to midodrine administration.
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http://dx.doi.org/10.23876/j.krcp.2018.37.1.85DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5875580PMC
March 2018

A comparative study on cardiovascular disease risk factors in Korean adults according to clinical depression status.

Authors:
Yuri Seo Youjin Je

Psychiatry Res 2018 05 26;263:88-93. Epub 2018 Feb 26.

Department of Food and Nutrition, Kyung Hee University, 26 Kyunghee-daero, Dongdaemun-gu, 02447 Seoul, South Korea. Electronic address:

This study was conducted to compare the association between depression and cardiovascular disease risk factors among Korean adults. This study was based on the data from the Korea National Health and Nutrition Examination Survey conducted in 2012-2014. Using a multivariable logistic regression model, we calculated odds ratios (ORs) and 95% confidence intervals (CIs). A total of 10,359 subjects aged 19-64 years were selected, and 432 subjects (74 men, 358 women) were included in the physician-diagnosed depression group. Several cardiovascular risk factors were associated with higher odds of clinical depression. For men, high waist circumference (≥91.3 cm) and body mass index (≥25.0 kg/m) were significantly associated with increased odds of depression, and high physical activity (≥50 MET h/week) was associated with decreased odds of depression. Men with dyslipidemia and metabolic syndrome had 2.43-fold and 2.0-fold higher odds of depression than those without the diseases. For women, current smokers had 2.25-fold higher odds of depression than nonsmokers, and frequent alcohol drinkers (≥4 times/week) also had 2.88-fold higher odds of depression than nondrinkers. Korean adults with clinical depression had a higher prevalence of some risk factors for cardiovascular diseases than those without depression.
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http://dx.doi.org/10.1016/j.psychres.2018.02.052DOI Listing
May 2018

Serum CYR61 Is Associated with Disease Activity in Graves' Orbitopathy.

Ocul Immunol Inflamm 2018 26;26(7):1094-1100. Epub 2017 May 26.

a Institute of Vision Research, Department of Ophthalmology , Yonsei University College of Medicine , Seoul , South Korea.

Purpose: To investigate the clinical implications of cysteine-rich angiogenic inducer 61 (CYR61) in Graves' orbitopathy (GO).

Methods: Sera from 52 GO patients, 23 Graves' disease (GD) patients, and 20 healthy controls, and orbital fat tissue samples from 12 of 52 GO patients and 8 control subjects were included for analysis. Concentrations of CYR61 were measured from sera with an enzyme-linked immunosorbent assay, and CYR61 mRNA expression levels were evaluated from orbital fat tissue with polymerase chain reaction.

Results: Serum CYR61 levels were higher in GO patients than in controls (p = 0.001). Patients with active GO showed higher CYR61 levels than those with inactive GO (p < 0.001) or GD (p = 0.004). Expression of CYR61 mRNA was 7.4-fold higher in patients with GO than in healthy controls (p < 0.001).

Conclusions: CYR61 could be an adjuvant biomarker associated with the inflammatory activity of GO.
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http://dx.doi.org/10.1080/09273948.2017.1319960DOI Listing
December 2018

Quantitative analysis of cell proliferation by a dye dilution assay: Application to cell lines and cocultures.

Cytometry A 2017 07 4;91(7):704-712. Epub 2017 Apr 4.

Center for Bioanalysis, Division of Metrology for Quality of Life, Korea Research Institute of Standards and Science, 267 Gajeong-ro, Yuseong-gu, Daejeon, 34113, Republic of Korea.

Cell proliferation represents one of the most fundamental processes in biological systems, thus the quantitative analysis of cell proliferation is important in many biological applications such as drug screening, production of biologics, and assessment of cytotoxicity. Conventional proliferation assays mainly quantify cell number based on a calibration curve of a homogeneous cell population, and therefore are not applicable for the analysis of cocultured cells. Moreover, these assays measure cell proliferation indirectly, based on cellular metabolic activity or DNA content. To overcome these shortcomings, a dye dilution assay employing fluorescent cell tracking dyes that are retained within cells was applied and was diluted proportionally by subsequent cell divisions. Here, it was demonstrated that this assay could be implemented to quantitatively analyze the cell proliferation of different types of cell lines, and to concurrently analyze the proliferation of two types of cell lines in coculture by utilizing cell tracking dyes with different spectral characteristics. The mean division time estimated by the dye dilution assay is compared with the population doubling time obtained from conventional methods and values from literature. Additionally, dye transfer between cocultured cells was investigated and it was found that it is a characteristic of the cells rather than a characteristic of the dye. It was suggested that this method can be easily combined with other flow cytometric analyses of cellular properties, providing valuable information on cell status under diverse conditions. © 2017 International Society for Advancement of Cytometry.
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http://dx.doi.org/10.1002/cyto.a.23105DOI Listing
July 2017

Sphingosine-1-phosphate is involved in inflammatory reactions in patients with Graves' orbitopathy.

Inflamm Res 2017 Jun 31;66(6):535-545. Epub 2017 Mar 31.

Department of Ophthalmology, Institute of Vision Research, Yonsei University College of Medicine, Seoul, South Korea.

Objective: Graves' orbitopathy (GO) is initiated by excessive amount of various inflammatory mediators produced by orbital fibroblasts. This study aimed to assess the crucial role of sphingosine-1-phosphate (S1P) in the inflammatory process of GO.

Methods: Orbital adipose/connective tissue samples were obtained from 10 GO patients and 10 normal control individuals during surgery. Primary orbital fibroblast culture was done. After the expression of S1P receptors and sphingosine kinase (SphK) was assessed with the treatment of interleukin (IL)-1β, we evaluated the expression of pro-inflammatory factors [intercellular adhesion molecule-1 (ICAM-1), cyclooxygenase-2 (COX-2) and IL-6] after treating S1P. S1P receptor antagonists and SphK 1 inhibitor were pretreated and the expression of the pro-inflammatory factors was assessed.

Results: IL-1β exacerbated the inflammatory process by enhancing the expression of S1P receptors and SphK in GO orbital fibroblasts. IL-1β also induced the expressions of ICAM-1, COX-2, and IL-6 in GO orbital fibroblasts, and these expressions were effectively inhibited by S1P receptor antagonists and SphK1 inhibitor.

Conclusion: S1P has an important role in the pathological inflammatory process of GO, which is mediated through the SphK1-S1P- S1P receptor pathway. SphK1 inhibitors and S1P receptors or antagonists could be potential approaches for controlling the inflammatory process of GO.
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http://dx.doi.org/10.1007/s00011-017-1037-3DOI Listing
June 2017

Central Retinal Vein Occlusion Associated with Ulcerative Colitis.

Optom Vis Sci 2016 12;93(12):1567-1570

*MD Departments of Ophthalmology (YS, JHK, SCL) and Internal Medicine (JJP), Gangnam Severance Hospital, Yonsei University College of Medicine, Gangnam-gu, Seoul, Republic of Korea.

Purpose: To report a case of central retinal vein occlusion without macular edema associated with ulcerative colitis and its novel treatment with intravitreal dexamethasone.

Case Report: A 40-year-old man with ulcerative colitis presented with sudden visual disturbances. An initial fundus examination showed subtle yellow-to-white patches within the inner retina of the right eye superotemporal to the fovea. There were intraretinal hemorrhages and cotton-wool spots within the superior vascular arcade and nasal to the optic disc. Despite initiation of systemic corticosteroids, 2 weeks later there was an increase in retinal hemorrhages, formation of cotton wool spots, and development of optic disc swelling in the right eye. The patient was eventually diagnosed with nonischemic central retinal vein occlusion associated with ulcerative colitis. He received sustained-release intravitreal dexamethasone, which led to the resolution of retinal hemorrhage, optic disc swelling, and cotton-wool spots. Three months after the injection, retinal hemorrhages were not detectable. However, ocular coherence imaging showed marked thinning of the inner retina at the locations that were previously hyper-reflective.

Conclusions: Central retinal vein occlusion is an uncommon ophthalmologic manifestation associated with ulcerative colitis. Injection of intravitreal dexamethasone could be a viable treatment option in these patients even without the presence of macular edema.
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http://dx.doi.org/10.1097/OPX.0000000000000971DOI Listing
December 2016

Reply to a letter to the editor: Diabetic Retinal Pigment Epitheliopathy: Fundus Autofluorescence and Spectral-Domain Optical Coherence Tomography Findings.

Graefes Arch Clin Exp Ophthalmol 2016 09 24;254(9):1867. Epub 2016 Jun 24.

Institute of Vision Research, Department of Ophthalmology, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-Gu, Seoul, South Korea, 03722.

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http://dx.doi.org/10.1007/s00417-016-3407-xDOI Listing
September 2016

Diabetic retinal pigment epitheliopathy: fundus autofluorescence and spectral-domain optical coherence tomography findings.

Graefes Arch Clin Exp Ophthalmol 2016 Oct 6;254(10):1931-1940. Epub 2016 Apr 6.

Department of Ophthalmology, Institute of Vision Research, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-Gu, Seoul, 03722, Korea.

Purpose: To describe the characteristics of an unfamiliar disease entity, diabetic retinal pigment epitheliopathy (DRPE), using fundus autofluorescence (FAF) and spectral-domain optical coherence tomography (SD-OCT).

Methods: This retrospective study included 17 eyes from 10 proliferative diabetic retinopathy (PDR) patients with granular hypo-autofluorescence and/or variable hyper-autofluorescence on FAF (DRPE group) and 17 eyes from 10 age- and sex-matched PDR patients without abnormal autofluorescence (PDR group). Eyes with diabetic macular edema were excluded. Visual acuity (VA), retinal thickness (RT), and choroidal thickness (CT) were compared between the groups.

Results: Eyes in the DRPE group had worse logMAR VA than eyes in the PDR group (0.369 ± 0.266 vs. 0.185 ± 0.119; P = 0.026). The thickness of the retinal pigment epithelium plus the inner segment/outer segment of the photoreceptors was reduced to a greater degree in the DRPE group than the PDR group (P < 0.001). Moreover, the thickness of the outer nuclear layer plus the outer plexiform layer was thinner in the DRPE group than in the PDR (P = 0.013). However, the thickness of the inner retina showed no differences between the two groups. CT was significantly thicker in the DRPE group than in the PDR group (329.00 ± 33.76 vs. 225.62 ± 37.47 μm; P < 0.001).

Conclusions: Eyes with DRPE showed reduced VA, a thinner outer retina, and thicker choroid in comparison with eyes with PDR. Alterations of autofluorescence on FAF and changes in the outer retinal thickness and CT on SD-OCT can be helpful for differentiating DRPE in patients with PDR.
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http://dx.doi.org/10.1007/s00417-016-3336-8DOI Listing
October 2016

Intravitreal Bevacizumab for Traumatic Choroidal Rupture.

Optom Vis Sci 2015 Oct;92(10):e363-7

*MD Department of Ophthalmology, Gangnam Severance Hospital, Yonsei University College of Medicine, Gangnam-gu, Seoul, Korea (MK, JHK, YS); and Institute of Vision Research, Department of Ophthalmology, Severance Hospital, Yonsei University College of Medicine, Seodaemun-gu, Seoul, Korea (HJK, SCL).

Purpose: To report a case of visual loss associated with traumatic choroidal rupture after blunt ocular trauma that was successfully treated with an early intravitreal bevacizumab injection despite the absence of choroidal neovascularization (CNV).

Case Report: A 14-year-old boy presented with visual disturbance in his left eye after sustaining an ocular contusion 4 weeks earlier. The best-corrected visual acuity (BCVA) in the left eye was 20/50. Funduscopic examination revealed macular choroidal rupture accompanied by subretinal hemorrhage. Optical coherence tomography (OCT) showed accumulation of subretinal fluid around a disrupted retinal pigment epithelium/Bruch membrane complex extending into the juxtafoveolar area, but there was no active leakage suggestive of CNV on fluorescein angiography. Intravitreal bevacizumab (1.25 mg) injection was performed to treat persistent serous retinal detachment at macula causing visual loss. There was a reduction of subretinal fluid and concomitant improvement of BCVA to 20/30 within 1 week after intravitreal bevacizumab injection. The BCVA recovered to 20/25 in the left eye after 4 weeks, and only a minimal amount of residual fluid remained according to OCT. Complete resolution of subretinal fluid was observed by OCT at the 6-week follow-up examination, and BCVA improved to 20/20. Good visual acuity (20/20) and stable macula were maintained in the left eye at 1 year of follow-up without recurrence of subretinal fluid accumulation or hemorrhage and CNV. There were no ocular or systemic complications associated with intravitreal bevacizumab injection.

Conclusions: Early intravitreal bevacizumab injection could be an effective treatment option for patients with vision loss associated with traumatic choroidal rupture and subretinal fluid within the posterior pole before development of CNV.
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http://dx.doi.org/10.1097/OPX.0000000000000677DOI Listing
October 2015

YAG Laser Membranotomy for Subinternal Limiting Membrane Hemorrhage.

Optom Vis Sci 2015 Jul;92(7):e154-7

*MD Department of Ophthalmology, Gangnam Severance Hospital, Yonsei University College of Medicine, Gangnam-gu, Seoul, Korea (MK, JSK, YS); and Institute of Vision Research, Department of Ophthalmology, Severance Hospital, Yonsei University College of Medicine, Seodaemun-gu, Seoul, Korea (HJK, SCL).

Purpose: To report the use of neodymium:yttrium-aluminum-garnet (Nd:YAG) laser internal limiting membranotomy to successfully treat the first reported case of subinternal limiting membrane (sub-ILM) hemorrhage that developed after coil embolization of a cerebral aneurysm.

Case Report: A 59-year-old Korean woman visited our clinic complaining of central scotoma in her left eye, which developed shortly after stent-assisted coil embolization of an unruptured internal carotid artery aneurysm. Fundus examination revealed a sub-ILM hemorrhage in her left eye, and after 2 days, a Nd:YAG laser membranotomy was performed with a single 4.5-mJ burst. There was a marked reduction in the sub-ILM hemorrhage 1 day after Nd:YAG laser membranotomy, and after 1 month, her best-corrected visual acuity improved to 20/20, with complete resolution of the sub-ILM hemorrhage.

Conclusions: Sub-ILM hemorrhage can develop after coil embolization of a cerebral aneurysm, and Nd:YAG laser internal limiting membranotomy can be a useful noninvasive treatment alternative to surgical intervention.
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http://dx.doi.org/10.1097/OPX.0000000000000621DOI Listing
July 2015

A case of paradoxical reaction after treatment of eyelid tuberculosis.

Korean J Ophthalmol 2014 Dec;28(6):493-5

Institute of Vision Research, Department of Ophthalmology, Yonsei University College of Medicine, Seoul, Korea.

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http://dx.doi.org/10.3341/kjo.2014.28.6.493DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4239469PMC
December 2014

Expression of Lymphangiogenic Markers in Rejected Human Corneal Buttons after Penetrating Keratoplasty.

Curr Eye Res 2015 Sep 20;40(9):902-12. Epub 2014 Oct 20.

Department of Ophthalmology, Institute of Vision Research, Yonsei University College of Medicine , Seoul , Korea .

Purpose: To investigate the extent and distribution of lymphangiogenesis in the rejected corneal graft, we determined the expression of several lymphangiogenic markers in rejected human corneal buttons.

Material And Methods: Thirty-four corneal buttons were obtained from patients who underwent re-keratoplasty for graft rejection after penetrating keratoplasty. All corneas showed signs of rejection, such as, sudden mutton-fat keratic precipitates (KPs) or lines before re-keratoplasty. The corneas were halved, and one half was used for immunostaining and the other half was used for RT-PCR. Expression of vascular endothelial growth factor (VEGF)-A, VEGF-C, VEGF-D, VEGFR-2, VEGFR-3, LYVE-1 and podoplanin were measured as lymphangiogenic markers. Four non-operated normal corneas were used as controls.

Results: Numerous podoplanin positive cells were found in the anterior and posterior stroma. However, LYVE-1 positive mature lymphatics were found only in herpetic keratitis (HK)-induced graft rejection, and not in pseudophakic bullous keratopathy (PBK). RT-PCR showed that levels of VEGF-A, VEGF-C, VEGFR-2, and VEGFR-3 mRNAs were elevated in rejected corneal buttons versus the non-operated control corneas. Based upon the pre-keratoplasty pathologic conditions, HK cases showed higher levels of VEGF-A and VEGFR-2 than PBK. The mRNA ratios (keratoplastic cornea/normal cornea) for VEGF-A and VEGFR-2 were 8.9 and 5.8, respectively.

Conclusions: The results suggested that the VEGF-A and the VEGFR-2 may be a more important pathway for lymphangiogenesis in rejected corneal grafts than the VEGFR-3. In addition, organized lymphangiogenesis is more prominent in HK than PBK.
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http://dx.doi.org/10.3109/02713683.2014.969809DOI Listing
September 2015

Dry eye-induced CCR7+CD11b+ cell lymph node homing is induced by COX-2 activities.

Invest Ophthalmol Vis Sci 2014 Sep 25;55(10):6829-38. Epub 2014 Sep 25.

Institute of Vision Research, Department of Ophthalmology, Yonsei University College of Medicine, Seoul, Korea Institute of Corneal Dystrophy Research, Department of Ophthalmology, Yonsei University College of Medicine, Seoul, Korea.

Purpose: We aimed to determine the role of CCR7+CD11b+ cell lymph node (LN) homing and T-cell differentiation in dry eye (DE)-induced immunopathogenesis and investigate the therapeutic effects of cyclooxygenase-2 (COX-2) and prostaglandin E2/eicosanoid-prostanoid (PGE2/EP) inhibitors against DE.

Methods: Six-week-old female C57BL/6 mice were housed in a controlled-environment chamber and administered topical selective COX-2 inhibitors or EP2 antagonists. Expression of major histocompatibility complex (MHC)-IIhigh, CD11b+, CCR7+, IFN-γ+, IL-17+, and CD4+ in the corneas and draining LNs was evaluated using flow cytometry. Mixed lymphocyte reactions (MLRs) with carboxyfluorescein diacetate succinimidyl ester labeling and intracellular cytokine staining were used to verify DE-induced corneal dendritic cell function. mRNA expression of COX-2, EPs, and proinflammatory cytokines in ocular surface was evaluated using quantitative RT-PCR and immunohistochemical staining.

Results: Dry eye significantly increased MHC-IIhighCD11b+ and CCR7+CD11b+ cells in the cornea and LNs, and MLR revealed CCR7+CD11b+ cells from DE corneas stimulated IL-17+CD4+ cell proliferation. mRNA levels of COX-2, EP2, IFN-γ, TNF-α, IL-6, and IL-17 were significantly higher in DE ocular surface but were suppressed by topical COX-2 inhibitors and EP2-specific blockers. Immunohistochemical staining showed COX-2 and matrix metalloproteinase expression in DE corneal epithelia that was diminished by both topical treatments. Furthermore, both topical treatments significantly reduced frequencies of MHC-IIhigh, CD11b+, and CCR7+CD11b+ cells in the corneas and LNs, but also IL-17+CD4+ cells in LNs.

Conclusions: Topical COX-2/EP2 treatment reduces CCR7+CD11b+ cells on the ocular surface with inhibition of cellular LN homing and suppresses Th17 immune response, suggesting the COX-2/PGE2/EP axis contributes to immuno-inflammatory pathogenesis on the ocular surface and may be a novel therapeutic target in DE.
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http://dx.doi.org/10.1167/iovs.14-14744DOI Listing
September 2014