Publications by authors named "Yuqing Sun"

164 Publications

Transcriptome Analysis Reveals the Response Mechanism of -Mediated Resistance to f. sp. (FORL) Infection in Tomato.

Int J Mol Sci 2022 Jun 25;23(13). Epub 2022 Jun 25.

College of Horticulture, Northeast Agricultural University, Harbin 150030, China.

Tomato crown and root rot (FCRR) is an extremely destructive soil-borne disease. To date, studies have shown that only plants with tomato mosaic virus (TMV) resistance exhibit similar resistance to tomato f. sp. (FORL) and have identified a single relevant gene, , in Peruvian tomato. Due to the relative lack of research on FCRR disease-resistance genes in China and elsewhere, transcriptome data for FORL-resistant (cv. '19912') and FORL-susceptible (cv. 'Moneymaker') tomato cultivars were analysed for the first time in this study. The number of differentially expressed genes (DEGs) was higher in Moneymaker than in 19912, and 189 DEGs in the 'plant-pathogen interaction' pathway were subjected to GO and KEGG enrichment analyses. and genes were enriched in major metabolic pathways related to plant disease resistance; thus, we focused on these two gene families. In the early stage of tomato infection, the content of JA and SA increased, but the change in JA was more obvious. Fourteen genes were selected for confirmation of their differential expression levels by qRT-PCR. This study provides a series of novel disease resistance resources for tomato breeding and genetic resources for screening and cloning FORL resistance genes.
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http://dx.doi.org/10.3390/ijms23137078DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9267026PMC
June 2022

Evaluation of culturable 'last-resort' antibiotic resistant pathogens in hospital wastewater and implications on the risks of nosocomial antimicrobial resistance prevalence.

J Hazard Mater 2022 Jun 28;438:129477. Epub 2022 Jun 28.

State Key Laboratory of Microbial Technology, Microbial Technology Institute, Shandong University, Qingdao, Shandong 266237, China. Electronic address:

Antimicrobial resistance has been recognized as an important emerging environmental pollutant. 'Last-resort' antibiotics including tigecycline, polymyxin E, daptomycin, vancomycin and linezolid are the 'last line of defence' for antibiotic resistant pathogen infections. Therefore, the presence of 'last-resort' antibiotic resistant pathogens in hospital environments and the nosocomial transmission of 'last-resort' antibiotic resistance poses a grave threat to the well-being of patients. In this work, the extent of resistance to 'last-resort' antibiotics in culturable pathogens in hospital wastewater was investigated. Resistance to 'last-resort' antibiotics were quantified for 1384 culturable Enterobacteriaceae, Enterococcus, Staphylococcus, and Pseudomonas strains. With these investigations, several significant findings were made: (1) a very high level of resistance to 'last-resort' antibiotics was found; (2) multiple resistance to antibiotics, including 'last-resort' antibiotics, was prevalent; (3) a high level of 'last-resort' antibiotic resistance phenotype-genotype inconsistency was found, suggesting knowledge gap for resistance mechanisms; 4) tet(X4)-containing tigecycline-resistant Gram-positive pathogens were found for the first time; 5) wastewater treatment processes are effective in preventing the release of 'last-resort' antibiotic resistant pathogens to the environment. This investigation reveals the severe situation on 'last-resort' resistance in the hospital environment, and implies high risk for nosocomial transmission of 'last-resort' antibiotic resistant pathogens.
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http://dx.doi.org/10.1016/j.jhazmat.2022.129477DOI Listing
June 2022

Electroactive Fe-biochar for redox-related remediation of arsenic and chromium: Distinct redox nature with varying iron/carbon speciation.

J Hazard Mater 2022 05 12;430:128479. Epub 2022 Feb 12.

Department of Civil and Environmental Engineering, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China; Research Centre for Resources Engineering towards Carbon Neutrality, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China. Electronic address:

Electroactive Fe-biochar has attracted significant attention for As(III)/Cr(VI) immobilization through redox reactions, and its performance essentially lies in the regulation of various Fe/C moieties for desired redox performance. Here, a series of Fe-biochar with distinct Fe/C speciation were rationally produced via two-step pyrolysis of iron minerals and biomass waste at 400-850 °C (BCX-Fe-Y, X and Y represented the first- and second-step pyrolysis temperature, respectively). The redox transformation of Cr(VI) and As(III) by Fe-biochar was evaluated in simulated wastewater under oxic or anoxic conditions. Results showed that more effective Cr(VI) reduction could be achieved by BCX-Fe-400, while a higher amount of As (III) was oxidized by BCX-Fe-850 under the anoxic environment. Besides, BCX-Fe-400 could generate more reactive oxygen species (e.g.,OH) by reducing the O, which enhanced the redox-related transformation of pollutants under the oxic situation. The evolving redox performance of Fe-biochar was governed by the transition of the redox state from reductive to oxidative related to the Fe/C speciation. The small-sized amorphous/low-crystalline ferrous minerals contributed to a higher electron-donating capacity (0.43-1.28 mmol g) of BCX-Fe-400. In contrast, the oxidative surface oxygen-functionalities (i.e., carboxyl and quinoid) on BCX-Fe-850 endowed a stronger electron-accepting capacity (0.71-1.39 mmol g). Moreover, the graphitic crystallites with edge-type defects and porous structure facilitated the electron transfer, leading to a higher electron efficiency of BCX-Fe-850. Overall, we unveiled the roles of both Fe and C speciation in maneuvering the redox reactivity of Fe-biochar, which can advance our rational design of electroactive Fe-biochar for redox-related environmental remediation.
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http://dx.doi.org/10.1016/j.jhazmat.2022.128479DOI Listing
May 2022

A Cost-Effective Way to Produce Gram-Scale O-Labeled Aromatic Aldehydes.

Org Lett 2022 Jun 14;24(24):4409-4414. Epub 2022 Jun 14.

Key Laboratory of Eco-functional Polymer Materials of the Ministry of Education, Key Laboratory of Eco-environmental Polymer Materials of Gansu Province, College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, China.

Obtaining O-labeled organic substances is of great research importance and also an extremely challenging work. In this work, depending on the reversed Knoevenagel reaction, O-labeled aromatic aldehydes () are successfully obtained with high total yields (52-72%) and sufficient O abundance (90.90-96.09%).
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http://dx.doi.org/10.1021/acs.orglett.2c01637DOI Listing
June 2022

Cumulative risk assessment of dietary exposure to phthalates in pregnant women in Beijing, China.

Environ Sci Pollut Res Int 2022 May 28. Epub 2022 May 28.

Department of Toxicology, School of Public Health, Peking University, Beijing, 100191, China.

Diet is an important exposure route for phthalates, such as di-iso-butyl phthalate (DiBP), dibutyl phthalate (DBP), bis(2-ethylhexyl) phthalate (DEHP), and benzyl butyl phthalate (BBP). In this study, we aimed to estimate phthalate exposure in the diet of pregnant women and assess the health risk. A total of 517 pregnant women in the first trimester were recruited, and food frequency questionnaires were collected. A simple distribution assessment method was used to estimate daily exposure, and the hazard index (HI) method was used to assess cumulative risk. The maximum daily dietary exposure to DEHP, DBP, DiBP, and BBP was 5.25, 3.17, 2.59, and 0.58 μg/kg bw/day, respectively, and did not exceed the safety limit values. Cereals and vegetables were the main sources of the estimated daily intake (EDI) of phthalates in the diet. The cumulative risk assessment, based on the European Food Safety Authority tolerable daily intake (TDI) and the US Environmental Protection Agency reference dose (RfD), did not exceed the threshold of 1. DiBP, DBP, and DEHP had higher hazard quotient (HQ) values for cumulative health risk than BBP. In conclusion, a low health risk was posed by the cumulative dietary exposure to phthalates for pregnant women in Beijing.
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http://dx.doi.org/10.1007/s11356-022-20151-yDOI Listing
May 2022

A review of physical, chemical, and biological green tide prevention methods in the Southern Yellow Sea.

Mar Pollut Bull 2022 Jul 24;180:113772. Epub 2022 May 24.

College of Marine Ecology and Environment, Shanghai Ocean University, Shanghai 201306, China; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Ocean University, Lianyungang 222005, China. Electronic address:

Green tides dominated by Ulva prolifera have be present in the Southern Yellow Sea for 15 consecutive years. They not only damage the marine environment, but also cause economic losses to coastal cities. However, there is still no fully effective approach for preventing green tides. In this article, approaches for the prevention of U. prolifera taken over recent years are reviewed. They can be generally divided into physical, chemical, and biological approaches. Physical approaches have been used to control the overwhelming green macroalgae bloom and inhibit the germination of U. prolifera, including physical salvage approach, refrigeration net technology, improved farming methods and raft technology, and modified clay method. These approaches require significant labor and material resources. Many chemical reagents have been used to eliminate U. prolifera early germination and growth, such as oxidative algaecide, acid treatment, heavy metal compounds, antifouling coating, and alkaloids. Chemical approaches have high efficiency, high economic benefit, and simple operation. Presently, biological control approaches remain in the exploratory stage. The verification of pilot and large-scale experiment results in sea areas is lacking, including the application of large organisms and microorganisms to control U. prolifera, and some of the mechanisms have not been thoroughly studied. This article introduces the three types of approaches, and evaluates the advantages and disadvantages of different methods to facilitate the reduction of the green tide bloom scale in the Southern Yellow Sea.
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http://dx.doi.org/10.1016/j.marpolbul.2022.113772DOI Listing
July 2022

Effects of ultrasound-assisted chitosan grafted caffeic acid coating on the quality and microbial composition of pompano during ice storage.

Ultrason Sonochem 2022 May 11;86:106032. Epub 2022 May 11.

College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China; Shanghai Aquatic Products Processing and Storage Engineering Technology Research Center, Shanghai 201306, China; National Experimental Teaching Demonstration Center for Food Science and Engineering (Shanghai Ocean University), Shanghai 201306, China. Electronic address:

The effects of ultrasound-assisted chitosan grafted caffeic acid coating on the quality and microbial composition of fresh pompano (Trachinotus ovatus) fillets during ice storage for 24 days were evaluated. Samples were treated by distilled water (CK), ultrasound (US), chitosan grafted caffeic acid coating (G), and chitosan grafted caffeic acid coating with ultrasound-assisted (USG). Results showed that samples treated with USG could inhibit the formation of corrupt substances such as TVB-N, TBA, biogenic amines (BAs), hypoxanthine (Hx), and hypoxanthine riboside (HxR) when compared to the CK group.The results of high-throughput sequencing technology observed that the major bacteria genus of fresh samples was Acinetobacter.The diversity of bacterial communities at the initial stage was more diverse than that at the end of stage. With the extension of storage time, the USG treatment could maintain the microbial diversity. The dominant microbiota was Shewanella and Brochothrix in the CK group after 24 days of storage. In addition, Brochothrix in treated groups was effectively decreased. The microbial communities of samples in all treatments were changed during storage. At the end of storage, there was a significant difference in bacterial composition between the CK and treated samples, indicating that the treatment can effectively inhibit the growth of microorganisms, especially spoilage microorganisms, and reduce the quality deterioration caused by bacteria.
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http://dx.doi.org/10.1016/j.ultsonch.2022.106032DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9130226PMC
May 2022

Analysis of the Effect of Velocity on the Eddy Current Effect of Metal Particles of Different Materials in Inductive Bridges.

Sensors (Basel) 2022 Apr 29;22(9). Epub 2022 Apr 29.

School of Marine Engineering, Dalian Maritime University, Dalian 116026, China.

A method for analyzing the influence of velocity changes on metal signals of different materials in oil detection technology is proposed. The flow rate of metal contaminants in the oil will have a certain impact on the sensitivity of the output particle signal in terms of electromagnetic fields and circuits. The detection velocity is not only related to the sensitivity of the output particle signal, but also to the adaptability of high-speed and high-throughput in oil online monitoring. In this paper, based on a high-sensitivity inductive bridge, the eddy current effect of velocity in a time-harmonic magnetic field is theoretically analyzed and experimentally verified, the phenomenon of particle signal variation with velocity for different materials is analyzed and discussed, and finally the effect of velocity on the output signal of the processing circuit is also elaborated and experimentally verified. Experiments show that under the influence of the time-harmonic magnetic field, the increase of the velocity enhances the detection sensitivity of non-ferromagnetic metal particles and weakens the detection sensitivity of non-ferromagnetic particles. Under the influence of the processing circuit, different velocities will produce different signal gains, which will affect the stability of the signal at different velocities.
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http://dx.doi.org/10.3390/s22093406DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9099596PMC
April 2022

Pear-Shaped Disk as a Risk Factor for Intraoperative End Plate Injury in Oblique Lumbar Interbody Fusion.

World Neurosurg 2022 May 13. Epub 2022 May 13.

Department of Spine Surgery, Peking University Fourth School of Clinical Medicine, Beijing Jishuitan Hospital, Beijing, People's Republic of China. Electronic address:

Background: Intraoperative end plate injury can result in late-onset cage subsidence in oblique lumbar interbody fusion (OLIF). This study aimed to identify risk factors for intraoperative end plate injury and investigate whether a pear-shaped disk correlated with intraoperative end plate injury in OLIF.

Methods: We retrospectively reviewed 102 levels in 82 patients (mean age 60.1 ± 10.0 years) who underwent OLIF for degenerative lumbar diseases. Intraoperative end plate injury was evaluated using midline sagittal computed tomography views at 3 days postoperatively and defined as cage breaching into an adjacent cortical end plate >2 mm. Patient demographics, surgical parameters, radiographic parameters, and cage-related parameters were recorded in all surgical levels. Evaluation of risk factors associated with intraoperative end plate injury was performed. Patient-reported outcome, fusion status, and late-onset cage subsidence were analyzed at a minimum of 1 year after the surgery.

Results: Intraoperative end plate injury was observed in 26 levels (25.5%). Multivariate logistic regression analysis identified that bone mineral density (odds ratio [OR] = 0.978), preoperative segmental lordosis (OR = 0.790), and pear-shaped disk were risk factors (OR = 5.837) for intraoperative end plate injury. Intraoperative end plate injury occurred in 45.5% of levels with a pear-shaped disk compared with 16.0% of levels with no pear-shaped disk (P < 0.01). Late-onset cage subsidence was significantly more frequent in the injury group than the no-injury group. Patient-reported outcome and fusion status were unrelated to intraoperative end plate injury.

Conclusions: A pear-shaped disk is the greatest risk factor for intraoperative end plate injury following OLIF.
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http://dx.doi.org/10.1016/j.wneu.2022.05.037DOI Listing
May 2022

Engineered Nissle 1917 with urate oxidase and an oxygen-recycling system for hyperuricemia treatment.

Gut Microbes 2022 Jan-Dec;14(1):2070391

State Key Laboratory of Microbial Technology, Shandong University, Qingdao, Shandong Province, China.

Hyperuricemia is the second most prevalent metabolic disease to human health after diabetes. Only a few clinical drugs are available, and most of them have serious side effects. The human body does not have urate oxidase, and uric acid is secreted via the kidney or the intestine. Reduction through kidney secretion is often the cause of hyperuricemia. We hypothesized that the intestine secretion could be enhanced when a recombinant urate-degrading bacterium was introduced into the gut. We engineered an Nissle 1917 strain with a plasmid containing a gene cassette that encoded two proteins PucL and PucM for urate metabolism from , the urate importer YgfU and catalase KatG from , and the bacterial hemoglobin Vhb from sp. The recombinant strain effectively degraded uric acid under hypoxic conditions. A new method to induce hyperuricemia in mice was developed by intravenously injecting uric acid. The engineered strain significantly lowered the serum uric acid when introduced into the gut or directly injected into the blood vessel. The results support the use of urate-degrading bacteria in the gut to treat hyperuricemia. Direct injecting bacteria into blood vessels to treat metabolic diseases is proof of concept, and it has been tried to treat solid tumors.
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http://dx.doi.org/10.1080/19490976.2022.2070391DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9067508PMC
May 2022

Prevention strategies for green tides at source in the Southern Yellow Sea.

Mar Pollut Bull 2022 May 12;178:113646. Epub 2022 Apr 12.

College of Marine Ecology and Environment, Shanghai Ocean University, Shanghai 201306, China. Electronic address:

As global ecological disasters, green tide outbreaks have been observed in the Southern Yellow Sea (SYS) of China since 2007, resulting in considerable economic losses and environmental damage to the coastal cities of Jiangsu and Shandong Provinces. Therefore, prevention of green tides is crucial. Previous studies have revealed that a relatively small green tide outbreak scale in the SYS was observed in 2018 and 2020, with the green tides covering areas of 193 km and 192 km and durations of 91 days and 64 days, respectively. Killing green macroalgae attached to cultivation ropes in Neopyropia aquaculture areas, which has been considered a primary source of the blooms, early removal of Neopyropia aquaculture rafts, and green tide prevention in the SYS are the key reasons for the decrease in green tides in 2018 and 2020. Furthermore, to address the challenges associated with the current green tide source prevention measures, we proposed a comprehensive control method that combines ecological farming, early green tide prevention, and resource utilization. Potential secondary pollution caused by the chemicals used to control Ulva prolifera can be minimized. Conversely, Neopyropia yezoensis quality may be enhanced through continuous improvement of its culturing process, which in turn, could reduce the green tide blooming scale.
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http://dx.doi.org/10.1016/j.marpolbul.2022.113646DOI Listing
May 2022

Controlling the main source of green tides in the Yellow Sea through the method of biological competition.

Mar Pollut Bull 2022 Apr 16;177:113561. Epub 2022 Mar 16.

College of Marine Ecology and Environment, Shanghai Ocean University, Shanghai 201306, China; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Ocean University, Lianyungang 222005, China; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China. Electronic address:

Macroalgal blooms have become a serious threat to public health, fisheries, ecosystems, and global economies. Since 2007, in the Yellow Sea, China, Ulva green tides have occurred for 15 consecutive years. However, effective control methods are limited. Ulva prolifera attached to Neopyropia aquaculture rafts are believed to be the main source of blooms, therefore eliminating Ulva from rafts could effectively prevent and control blooms. We investigated this phenomenon and showed that macroalgae germination was significantly inhibited by dried Neopyropia yezoensis at concentrations of 1.2, 2.4, and 4.8 g DW. Also, the inhibitory effects of dried N. yezoensis toward U. prolifera gametes at 2.4 and 4.8 g DW were >90% at day 21. N. yezoensis culture filtrates and thalli were also used to determine dose-dependent inhibition effects on U. prolifera gamete germination. Both were potent and significantly inhibited germination at 1.75-7 g FW; the inhibitory effect 7 g FW was >90% at day 21. As N. yezoensis thalli exhibited high inhibitory effects in laboratory experiments, we also performed field studies. N. yezoensis on ropes displayed high inhibitory effects on Ulva attachment and growth. Thus N. yezoensis powder, culture filtrates, and thalli displayed strong inhibitory effects on U. prolifera gametes, suggesting N. yezoensis attachment to ropes could be used to control green tides at the source.
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http://dx.doi.org/10.1016/j.marpolbul.2022.113561DOI Listing
April 2022

Chitosan-grafted-phenolic acid copolymers against Shewanella putrefaciens by disrupting the permeability of cell membrane.

World J Microbiol Biotechnol 2022 Mar 15;38(4):73. Epub 2022 Mar 15.

College of Food Science and Technology, Shanghai Ocean University, Shanghai, 201306, China.

Chitosan (CS) is a kind of high molecular polymer with antibacterial properties. A copolymer with high bacteriostatic activity can be formed by grafting phenolic acid compounds into the chitosan molecular chain, which can inhibit the growth of dominant spoilage bacteria in aquatic products. The study aimed to investigate the antibacterial effect and mechanism of chitosan-grafted-phenolic acid copolymers on Shewanella putrefaciens (S. putrefaciens). CS-grafted-protocatechuic acid (CS-g-PA) and CS-grafted-gallic acid (CS-g-GA) were attained by EDC/NHS coupling reaction. The antibacterial tests indicated that CS-g-PA and CS-g-GA had the same minimum inhibitory concentration (MIC) (1.25 mg/mL) and minimum bactericidal concentration (MBC) (5.0 mg/mL) against S. putrefaciens. According to the change trend of growth curve, the growth of S. putrefaciens was significantly restrained under 2MIC graft copolymers (P < 0.05). Moreover, the increment of alkaline phosphatase (AKPase) activity and electrical conductivity demonstrated that the cell wall and membrane permeability of S. putrefaciens were damaged respectively. In addition, the increase of lactate dehydrogenase (LDHase) activity, protein and nucleic acid absorbance and the decrease of adenosine triphosphatase (ATPase) activity suggested that the cell membrane was incomplete and poor fluidity. The irregular shape of bacteria and the outflow of intercellular contents were also observed from scanning electron microscope (SEM). The above results manifested a great potential of CS-g-PA and CS-g-GA for use as food preservatives to aquatic products.
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http://dx.doi.org/10.1007/s11274-022-03261-0DOI Listing
March 2022

Research on High Sensitivity Oil Debris Detection Sensor Using High Magnetic Permeability Material and Coil Mutual Inductance.

Sensors (Basel) 2022 Feb 25;22(5). Epub 2022 Feb 25.

School of Marine Engineering, Daliann Maritime University, Dalian 116026, China.

Metallic contaminants (solid) are generated by friction pair, causing wear of equipment by enters the lubricating system. This poses a great potential threat to the normal operation of such machines. The timely analysis and detection of debris can lead to the avoidance of mechanical failures. Abnormal wear in machinery may produce debris exceeding 10 μm. The traditional inductance detection method has low sensitivity and cannot meet the actual detection requirements. To boost the sensitivity of the inductance sensor, the mutual inductance of coils and the strong magnetic conductivity of permalloy was utilized to design a high sensitivity inductance sensor for the detection of debris in lubricating oil. This design was able to detect 10-15 μm iron particles and 65-70 μm copper particles in the oil. The experimental results illustrate that low-frequency excitation is the best for detecting ferromagnetic particles, while high-frequency excitation has the best effect for detecting non-ferromagnetic particles. This paper demonstrates the significant advantages of coil mutual inductance, and strong magnetic conductivity of permalloy in improving the detection sensitivity of oil debris sensors. This will provide technical support for wear detection in mechanical equipment and fault diagnosis.
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http://dx.doi.org/10.3390/s22051833DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8914667PMC
February 2022

Structural characterization and antibacterial properties of konjac glucomannan/soluble green tea powder blend films for food packaging.

J Food Sci Technol 2022 Feb 26;59(2):562-571. Epub 2021 Feb 26.

Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Institute of Aquaculture and Protection of Waters, University of South Bohemia in České Budějovice, Na Sádkách 1780, 37005 České Budějovice, Czech Republic.

Antimicrobial activity is a promising property for food packaging which could prolong the shelf life of food products. In this paper, the physicochemical and antimicrobial properties of konjac glucomannan (KGM)/soluble green tea powder (SGTP) edible films were firstly prepared and analyzed through light barrier properties, Fourier transform infrared spectroscopy (FT-IR), tensile strength (TS), X-ray diffraction (XRD), thermogravimetric analysis and scanning electron microscope (SEM). The results showed that appropriate addition of SGTP could improve the TS of composite films. With the increase of SGTP content, the transmittance of the films in the ultraviolet region decreased obviously, and the thermal stability was improved in a SGTP dependent manner. KGM/SGTP films present a fairly smooth and flat surface without any fracture when 0.5% SGTP was provided. The bacteriostatic test showed that the bacteriostatic performance of the composite films against and was also significantly enhanced. When 1% SGTP was provided, the zones of inhibition for and reached to 13.45 ± 0.94 mm and 13.76 ± 0.92 mm, respectively. Overall, the KGM/SGTP films showed great potential as bioactive packaging materials to extend food shelf life.
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http://dx.doi.org/10.1007/s13197-021-05041-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8814267PMC
February 2022

Vinasse-based biochar magnetic composites: adsorptive removal of tetracycline in aqueous solutions.

Environ Sci Pollut Res Int 2022 Feb 10. Epub 2022 Feb 10.

College of Resources and Environment, Hunan Agricultural University, Changsha, 410128, China.

Highly efficient and cost-effective adsorbents for antibiotic removal are the key to mitigate pollution by industrial wastewaters. Pyrolyzing low-cost winemaking waste into biochar is a promising means for waste biomass utilization. This study assembled vinasse-derived biochar with manganese ferrite into vinasse-manganese ferrite biochar-magnetic composites (V-MFB-MCs) through simultaneous pyrolysis of waste biomass and metal (Mn and Fe) hydroxide precipitates. Batch experiments were conducted to evaluate the kinetics and isotherms of tetracycline (TC) adsorption as well as the influence of pH value, humic acid, and ionic strength. Morphological characterization showed that crystalline MnFeO nanoparticles were impregnated within the framework of fabricated V-MFB-MCs. Superior TC adsorption capacity and fast pseudo-second-order kinetics could be achieved by the V-MFB-MCs-800 at pH 3.0. The TC adsorption onto V-MFB-MCs-800 was highly pH-dependent and controlled by the positive influence of ionic strength and humic acid. V-MFB-MCs-800 showed excellent adsorption performance in different natural water. Multiple interaction mechanisms including pore filling effect, π-π stacking interaction, and hydrogen bonding contribute to TC removal by V-MFB-MCs-800, which can be an innovative biowaste-derived material for industrial wastewater treatment.
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http://dx.doi.org/10.1007/s11356-022-19012-5DOI Listing
February 2022

Dual-Aptamer-Targeted Immunomagnetic Nanoparticles to Accurately Explore the Correlations between Circulating Tumor Cells and Gastric Cancer.

ACS Appl Mater Interfaces 2022 Feb 1;14(6):7646-7658. Epub 2022 Feb 1.

Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou 221004, Jiangsu, China.

It has been acknowledged that circulating tumor cells (CTCs) are promising biomarkers in liquid biopsy for cancer diagnosis and prognosis. However, the relationship between the CTC number and gastric cancer has scarcely been quantitatively investigated. Moreover, the single criterion of epithelial cell adhesion molecule (EpCAM) antibody/aptamer to specifically recognize epithelial CTCs cannot be universally applied for clinical applications, as it fails to recognize EpCAM-negative CTCs. Herein, we propose simple, low-cost, dual-aptamer (EpCAM and PTK7)-modified immunomagnetic FeO particles (IMNs) for efficient capture of heterogeneous CTCs and downstream analysis in gastric cancer patients. High PTK7 expression and a significant negative correlation between PTK7 and EpCAM expression were observed in primary gastric cancer tissues. Taking MGC-803 and BGC-823 cells as CTC models, the obtained dual-targeting IMNs could distinguishably recognize these cells with both high or low EpCAM and PTK7 expressions, which enhanced the accuracy of CTC recognition in gastric cancer. More than 95% of these two kinds of cells could be captured within 20 min of incubation, which was significantly more efficient than that of single EpCAM- or PTK7-modified IMNs. With this strategy, as low as five CTCs could be captured from phosphate-buffered saline (PBS), a cell mixture containing THP-1 cells, and lysed blood mediums. Moreover, the obtained CTCs can be used for subsequent gene analysis. Finally, the fabricated IMNs were successfully applied for CTC capture in 1.0 mL of peripheral blood samples from patients with gastric cancer. The detected CTC numbers in 72 participants were found to have close relationships with chemotherapy sensitivity, diagnosis, stage, and distant metastasis of patients. This work provides important references for further investigations on CTC-related diagnosis and individualized treatment.
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http://dx.doi.org/10.1021/acsami.1c22720DOI Listing
February 2022

Regional Radiomics Similarity Networks Reveal Distinct Subtypes and Abnormality Patterns in Mild Cognitive Impairment.

Adv Sci (Weinh) 2022 04 31;9(12):e2104538. Epub 2022 Jan 31.

Beijing Advanced Innovation Centre for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang University, Beijing, 100191, China.

Individuals with mild cognitive impairment (MCI) of different subtypes show distinct alterations in network patterns. The first aim of this study is to identify the subtypes of MCI by employing a regional radiomics similarity network (R2SN). The second aim is to characterize the abnormality patterns associated with the clinical manifestations of each subtype. An individual-level R2SN is constructed for N = 605 normal controls (NCs), N = 766 MCI patients, and N = 283 Alzheimer's disease (AD) patients. MCI patients' R2SN profiles are clustered into two subtypes using nonnegative matrix factorization. The patterns of brain alterations, gene expression, and the risk of cognitive decline in each subtype are evaluated. MCI patients are clustered into "similar to the pattern of NCs" (N-CI, N = 252) and "similar to the pattern of AD" (A-CI, N = 514) subgroups. Significant differences are observed between the subtypes with respect to the following: 1) clinical measures; 2) multimodal neuroimaging; 3) the proportion of progression to dementia (61.54% for A-CI and 21.77% for N-CI) within three years; 4) enriched genes for potassium-ion transport and synaptic transmission. Stratification into the two subtypes provides new insight for risk assessment and precise early intervention for MCI patients.
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http://dx.doi.org/10.1002/advs.202104538DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9036024PMC
April 2022

Stoichiometric carbocatalysis via epoxide-like C-S-O configuration on sulfur-doped biochar for environmental remediation.

J Hazard Mater 2022 04 5;428:128223. Epub 2022 Jan 5.

Department of Civil and Environmental Engineering, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China; Research Centre for Resources Engineering towards Carbon Neutrality, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China. Electronic address:

Heteroatom doping is a promising technique to enhance biochar for effective environmental remediation. However, development of electroactive heteroatom-doped biochars, e.g., sulfur-doped biochar, has been hindered due to complex nature of non-stoichiometric biomass-derived carbon and changeable electrochemical state of dopants. Herein, we produced a series of wood waste-derived biochars with customized levels of minerals and redox-active moieties, aiming to unravel the crucial factors for sulfur doping. Calcium (Ca) in biochar was found to preferentially coordinate with sulfur to form inactive inorganic sulfur minerals (i.e., CaSO and CaS) with inferior catalytic reactivity. After diminishing the inherent Ca minerals beforehand, we could introduce surface phenoxyl-type radicals (C-O) and vacancy defects on the biochar to develop an electrophilic C-S-O bonding configuration, which guaranteed a high affinity towards peroxymonosulfate (PMS, 2.08 mM g, 30 min) and efficient removal of bisphenol A (BPA, 91.1%, 30 min). Scavenging experiments and in-situ Raman analyses indicated that the epoxide-like C-S-O structure induced nucleophilic addition of PMS to generate surface-bound singlet oxygen (O, major) and hydroxyl radicals (OH, minor) through a preservative and stoichiometric interfacial reaction. Overall, the proposed approach overcomes the major hurdles in science-informed fabrication of sulfur-doped biochar and advances its development for environmental remediation.
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http://dx.doi.org/10.1016/j.jhazmat.2022.128223DOI Listing
April 2022

A Pathway-Specific Polygenic Risk Score Is Associated with Tau Pathology and Cognitive Decline.

J Alzheimers Dis 2022 ;85(4):1745-1754

State Key Laboratory of Cognitive Neuroscience and Learning, Beijing Normal University, Beijing, China.

Background: Tauopathy is a primary neuropathological hallmark of Alzheimer's disease with a strong relationship to cognitive impairment. In the brain, tau aggregation is associated with the regulation of tau kinases and the binding ability of tau to microtubules.

Objective: To explore the potential for using specific polygenic risk scores (PRSs), combining the genetic influences involved in tau-protein kinases and the tau-protein binding pathway, as predictors of tau pathology and cognitive decline in non-demented individuals.

Methods: We computed a pathway-specific PRS using summary statistics from previous large-scale genome-wide association studies of dementia. We examined whether PRS is related to tau uptake in positron emission tomography (PET), tau levels, and the rate of tau level changes in cerebrospinal fluid (CSF). We further assessed whether PRS is associated with memory impairment mediated by CSF tau levels.

Results: A higher PRS was related to elevated CSF tau levels and tau-PET uptake at baseline, as well as greater rates of change in CSF tau levels. Moreover, PRS was associated with memory impairment, mediated by increased CSF tau levels. The association between PRS and tau pathology was significant when APOE was excluded, even among females. However, the effect of PRS on cognitive decline appeared to be driven by the inclusion of APOE.

Conclusion: The influence of genetic risk in a specific tau-related biological pathway may make an individual more susceptible to tau pathology, resulting in cognitive dysfunction in an early preclinical phase of the disease.
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http://dx.doi.org/10.3233/JAD-215163DOI Listing
March 2022

A Transcriptomic Analysis of Gonads from the Low-Temperature-Induced Masculinization of .

Animals (Basel) 2021 Nov 30;11(12). Epub 2021 Nov 30.

Key Laboratory of Mariculture, Agriculture Ministry, PRC, Dalian Ocean University, Dalian 116023, China.

The phenotypic sex of fish is usually plastic. Low-temperature treatment induces the masculinization of , resulting in pseudo-males (PM) with the physiological sex of a male (M) and genetic sex of a female (F). For a comparison of gonadal transcriptomes, we collected gonads from three groups of (F, M, and PM) for high-throughput transcriptome sequencing. The results provided 467,640,218 raw reads (70.15 Gb) and a total of 436,151,088 clean reads (65.43 Gb), with an average length of 150 bp. Only 79 differentially expressed genes (DEGs) were identified between F and PM, whereas 12,041 and 11,528 DEGs were identified between F and M, and PM and M, respectively. According to the functional annotation of DEGs, 13 DEGs related to gonadal development were screened (, , , , , , , , , , , , and ) which might participate in formating PM. A quantitative PCR of the DEGs confirmed the reliability of the RNA-seq. Our results provide an important contribution to the genome sequence resources for and insight into the molecular mechanism of masculinization in a cultured fish subject to low-temperature treatment.
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http://dx.doi.org/10.3390/ani11123419DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8697924PMC
November 2021

Ulva macroalgae within local aquaculture ponds along the estuary of Dagu River, Jiaozhou Bay, Qingdao.

Mar Pollut Bull 2022 Jan 14;174:113243. Epub 2021 Dec 14.

College of Marine Ecology and Environment, Shanghai Ocean University, Shanghai 201306, China. Electronic address:

Green macroalgal blooms caused by Ulva species have influenced the Shandong Province for 15 consecutive years since 2007, leading to serious damage to the marine environment. Great biomasses of attached Ulva prolifera on Neopyropia aquaculture rafts in the Yellow Sea were considered as sources of blooms. However, it is still unclear whether U. prolifera could survive and settle in the Qingdao coastal environment, Shandong, thus leading to local Ulva blooms in the future. In this research, sampling of Ulva macroalgae in seven Portunus trituberculatus aquaculture ponds along the coast of Qingdao was conducted on August 21, 2019. In total, 24 samples collected from the ponds were analyzed through methods of molecular biological identification (ITS, 5S, and rps2-trnL sequences) and genetic analysis. All the aquaculture ponds contained large amounts of floating Ulva macroalgae, which consisted of three species: U. prolifera, Ulva meridionalis, and Ulva pertusa. Among these species, U. meridionalis, which is usually found in southern Japan, also causes the green tide. In addition, all Ulva macroalgae floated on the surfaces of aquaculture ponds, and were discarded into the local coastal area by aquaculturist. This research raised our awareness of the importance of controlling the spread of the green tide related macroalgae.
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http://dx.doi.org/10.1016/j.marpolbul.2021.113243DOI Listing
January 2022

Association Between Left Ventricular Global Function Index and Outcomes in Patients With Dilated Cardiomyopathy.

Front Cardiovasc Med 2021 16;8:751907. Epub 2021 Nov 16.

Department of Radiology, Beijing Anzhen Hospital, Capital Medical University, Beijing, China.

Left ventricular global function index (LVGFI) assessed using cardiac magnetic resonance (CMR) seems promising in the prediction of clinical outcomes. However, the role of the LVGFI is uncertain in patients with heart failure (HF) with dilated cardiomyopathy (DCM). To describe the association of LVGFI and outcomes in patients with DCM, it was hypothesized that LVGFI is associated with decreased major adverse cardiac events (MACEs) in patients with DCM. This prospective cohort study was conducted from January 2015 to April 2020 in consecutive patients with DCM who underwent CMR. The association between outcomes and LVGFI was assessed using a multivariable model adjusted with confounders. LVGFI was the primary exposure variable. The long-term outcome was a composite endpoint, including death or heart transplantation. A total of 334 patients (mean age: 55 years) were included in this study. The average of CMR-LVGFI was 16.53%. Over a median follow-up of 565 days, 43 patients reached the composite endpoint. Kaplan-Meier analysis revealed that patients with LVGFI lower than the cutoff values (15.73%) had a higher estimated cumulative incidence of the endpoint compared to those with LVGFI higher than the cutoff values ( = 0.0021). The hazard of MACEs decreased by 38% for each 1 SD increase in LVGFI (hazard ratio 0.62[95%CI 0.43-0.91]) and after adjustment by 46% (HR 0.54 [95%CI 0.32-0.89]). The association was consistent across subgroup analyses. In this study, an increase in CMR-LVGFI was associated with decreasing the long-term risk of MACEs with DCM after adjustment for traditional confounders.
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http://dx.doi.org/10.3389/fcvm.2021.751907DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8635067PMC
November 2021

Ethionine-mediated reduction of S-adenosylmethionine is responsible for the neural tube defects in the developing mouse embryo-mediated m6A modification and is involved in neural tube defects via modulating Wnt/β-catenin signaling pathway.

Epigenetics Chromatin 2021 12 4;14(1):52. Epub 2021 Dec 4.

Department of Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan, 030001, Shanxi, China.

Neural tube defects (NTDs) remain one of the most life-threatening birth defects affecting infants. Most patients with NTDs eventually develop lifelong disability, which cause significant morbidity and mortality and seriously reduce the quality of life. Our previous study has found that ethionine inhibits cell viability by disrupting the balance between proliferation and apoptosis, and preventing neural stem cells from differentiating into neurons and astrocytes. However, how ethionine participates in the pathogenesis of neural tube development through N6-methyladenosine (m6A) modification remains unknown. This study aims to investigate METTL3- and ALKBH5-mediated m6A modification function and mechanism in NTDs. Herein, our results demonstrate that SAM play not only a compensatory role, it also leads to changes of m6A modification in neural tube development and regulation. Additionally, these data implicate that METTL3 is enriched in HT-22 cells, and METTL3 knockdown reduces cell proliferation and increases apoptosis through suppressing Wnt/β-catenin signaling pathway. Significantly, overexpression of ALKBH5 can only inhibit cell proliferation, but cannot promote cell apoptosis. This research reveals an important role of SAM in development of NTDs, providing a good theoretical basis for further research on NTDs. This finding represents a novel epigenetic mechanism underlying that the m6A modification has profound and lasting implications for neural tube development.
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http://dx.doi.org/10.1186/s13072-021-00426-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8645112PMC
December 2021

An Impedance Sensor for Distinguishing Multi-Contaminants in Hydraulic Oil of Offshore Machinery.

Micromachines (Basel) 2021 Nov 17;12(11). Epub 2021 Nov 17.

Marine Engineering College, Dalian Maritime University, Dalian 116026, China.

The cleanliness of hydraulic oil can reflect the service life of the oil and the wear state of hydraulic machinery. An impedance sensor is proposed to distinguish multi-contaminants in hydraulic oil. The impedance sensor has two detection modes: the inductance-resistance mode is used to detect metal debris, and the capacitance mode is used to distinguish water droplets and air bubbles. By adding a built-in silicon steel strip and an external silicon steel strip with high magnetic permeability, the distribution area, strength, and uniformity of the magnetic field are enhanced to improve the detection sensitivity under inductance and resistance parameters. In addition, the silicon steel strips are used as electrode plates to introduce capacitance parameter detection. The experimental results show that the resistance detection method based on coil successfully improves the detection ability for non-ferromagnetic metal debris. The impedance sensor for distinguishing multi-contaminants in hydraulic oil can provide technical support for fault diagnosis of offshore hydraulic machinery.
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http://dx.doi.org/10.3390/mi12111407DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8617831PMC
November 2021

miR-222-3p is involved in neural tube closure by directly targeting Ddit4 in RA induced NTDs mouse model.

Cell Cycle 2021 11 15;20(22):2372-2386. Epub 2021 Nov 15.

Department of Biochemistry and Molecular Biology, Shanxi Key Laboratory of Birth, Defect and Cell Regeneration, Shanxi Medical University, Taiyuan, Shanxi, China.

Previously our results showed miR-222-3p was significantly downregulated in retinoic acid-induced neural tube defect (NTD) mouse model through transcriptome. Down-regulation of miR-222-3p may be a causative biomarker in NTDs. In this study, RNA was extracted from mouse embryos at E8.5, E9.5 and E10.5, and the expression level of miR-222-3p was measured by quantitative real-time PCR analysis. The preliminary mechanism of miR-222-3p in NTDs involved in cell proliferation, apoptosis and migration was investigated in mouse HT-22 cell line. The expression of miR-222-3p was significantly decreased at E8.5, E9.5 and E10.5 developed in mouse embryos which were consistent with our transcriptome sequencing. Suppression of miR-222-3p in HT-22 cells resulted in the inhibition of cell proliferation and migration, cell cycle and apoptosis. Moreover, DNA damage transcript 4 (Ddit4) was identified as a direct and functional target of miR-222-3p. miR-222-3p is negatively regulated by Ddit4. The mutation of binding site of Ddit4 3'UTR abrogated the responsiveness of luciferase reporters to miR-222-3p and showed that Ddit4 expression partially attenuated the function of miR-222-3p. We preliminatively confirmed that low expression of miR-222-3p has reduced the expression of β-catenin, TCF4 and other related genes in the Wnt/β-catenin signaling pathway.Collectively, these results demonstrated that miR-222-3p regulates the Wnt/β-catenin signaling pathway through Ddit4 inhibition in HT-22 cells, resulted in cell proliferation and apoptosis imbalance, and thus led to neural tube defects.
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http://dx.doi.org/10.1080/15384101.2021.1982506DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8794519PMC
November 2021

Preparation of chitosan grafted caffeic acid coating and its effect on pompano (Trachinotus ovatus) preservation.

J Sci Food Agric 2022 May 18;102(7):2835-2845. Epub 2021 Nov 18.

College of Food Science and Technology, Shanghai Ocean University, Shanghai, China.

Background: The present study aimed to investigate the preservative effect of chitosan-caffeic acid grafts coating (CS-g-CA) on the quality and microbial characteristics of pompano (Trachinotus ovatus) during iced storage. CS-g-CA was prepared by a 1-(3-dimethylaminopropyl)-3-ethylcarbodiimidehydro/N-hydroxysuccinimide coupling reaction. The grafting of CS-g-CA was confirmed by UV-visible and Fourier-transform infrared spectra. Samples were treated with distilled water (control), chitosan (CS), caffeic acid (CA) and CS-g-CA for 10 min, respectively. Microbiological [total viable count (TVC), H S-producing bacteria count, Pseudomonas bacteria count], physicochemical indicators [water holding capacity (WHC), cooking loss, pH, total volatile basic nitrogen (TVB-N), thiobarbituric acid (TBA), texture profile analysis, free amino acids] and sensory evaluation were investigated during ice storage at 4 °C for up to 27 days.

Results: The results showed that the antioxidant and antibacterial activities of CS could be improved by grafting CA onto CS. CS-g-CA coating could greatly slow down the speed of water loss and maintain WHC. Furthermore, CS-g-CA coating showed superior antibacterial activities by inhibiting the growth of TVC, delayed the decline of flavor amino acids and reduced sensory change. In addition, CS-g-CA coating reduced lipid oxidation and protein degradation as indicated by the decrease in TBA and TVB-N, possibly as a result of the addition of CA into CS membrane significantly improving the antioxidant activity of CS.

Conclusion: Compared with the control group, CS-g-CA coating had the optimal effect and could enhance the shelf-life of Trachinotus ovatus for at least another 9 days. © 2021 Society of Chemical Industry.
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http://dx.doi.org/10.1002/jsfa.11624DOI Listing
May 2022

Interactions between biochar and clay minerals in changing biochar carbon stability.

Sci Total Environ 2022 Feb 22;809:151124. Epub 2021 Oct 22.

Department of Civil and Environmental Engineering, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, PR China. Electronic address:

The science-informed design of stable carbonaceous materials as 'green' soil amendment will be indispensable for improving the soil fertility and carbon sequestration ability. In this study, a series of biochars were prepared from mineral-rich cellulosic corn straw (C), lignocellulosic pine wood (P), and lignin-rich walnut shell (W) at 500 or 700 °C. Their change of carbon stability after 90-day interaction with two typical soil clay minerals (i.e., kaolinite or montmorillonite) under a field-relevant condition (mass ratio of biochar to soil clay at 1:5) was evaluated as carbon loss (%) determined by the KCrO-HSO oxidation method. The spectroscopic analyses demonstrated that the highly graphitized and microporous W-biochars exhibited a high carbon stability (35.6-40.2% C loss) that could be further enhanced in the presence of kaolinite or montmorillonite. This promotion was probably ascribed to the transformation from the aromatic CC/CC functionality to the ester CO and methyl CH configurations on the biochar surface forming stable organo-mineral complexes (i.e., COAl) with the clay minerals. In contrast, a substantial level of labile C fraction was observed in the C- and P-biochars (e.g., 94.8% C loss of P700-M) after incubation with the clay minerals, especially for montmorillonite with high CEC, swelling capacity, and week interlayer bonding. This adverse impact was possibly attributed to the aliphatic CC/CC bonding with low oxidation resistance after co-precipitation with the clay minerals. The results of this study can provide deeper insight into the evolution of physicochemical properties, porous structure, and carbon interactions during long-term biochar application for carbon sequestration and sustainable development.
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http://dx.doi.org/10.1016/j.scitotenv.2021.151124DOI Listing
February 2022

Additional lateral plate fixation has no effect to prevent cage subsidence in oblique lumbar interbody fusion.

J Orthop Surg Res 2021 Oct 11;16(1):584. Epub 2021 Oct 11.

Department of Spine Surgery, Peking University Fourth School of Clinical Medicine, Beijing Jishuitan Hospital, No. 31, Xinjiekou East Street, Xicheng District, Beijing, 100035, People's Republic of China.

Background: For lumbar degenerative diseases, cage subsidence is a serious complication and can result in the failure of indirect decompression in the oblique lumbar interbody fusion (OLIF) procedure. Whether additional lateral plate fixation was effective to improve clinical outcomes and prevent cage subsidence was still unknown. This study aimed to compare the incidence and degree of cage subsidence between stand-alone oblique lumbar interbody fusion (SA-OLIF) and OLIF combined with lateral plate fixation (OLIF + LP) for the treatment of lumbar degenerative diseases and to evaluate the effect of the lateral plate fixation.

Methods: This was a retrospective comparative study. 20 patients with 21 levels underwent SA-OLIF and 21 patients with 26 levels underwent OLIF + LP. We compared clinical and radiographic outcomes between two groups. Clinical evaluation included Visual Analog Scale (VAS) for back pain and leg pain, Japanese Orthopaedic Association (JOA) scores and Oswestry Disability Index (ODI). Radiographical evaluation included disc height (DH), segmental lordosis angle (SL), and subsidence rate on standing lateral radiographs. Cage subsidence was classified using Marchi's criteria.

Results: The mean follow-up duration was 6.3 ± 2.4 months. There were no significant differences among perioperative data (operation time, estimated intraoperative blood loss, and complication), clinical outcome (VAS, ODI, and JOA) and radiological outcome (SH and SL). The subsidence rate was 19.0% (4/21) in SA-OLIF group and 19.2% (5/26) in OLIF + LP group. 81.0% in SA-OLIF group and 80.8% in OLIF + LP group had Grade 0 subsidence, 14.3% in SA-OLIF group and 15.4% in OLIF + LP group had Grade I subsidence, and 4.8% in SA-OLIF group and 3.8% in OLIF + LP group had Grade II subsidence (P = 0.984). One patient with severe cage subsidence and lateral plate migration underwent revision surgery.

Conclusions: The additional lateral plate fixation does not appear to be more effective to prevent cage subsidence in the oblique lumbar interbody fusion, compared with stand-alone technique. If severe cage subsidence occurs, it may result in lateral plate migration in OLIF combined with lateral plate fixation.
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http://dx.doi.org/10.1186/s13018-021-02725-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8507130PMC
October 2021

Prescribed-Time Consensus Tracking of Multiagent Systems With Nonlinear Dynamics Satisfying Time-Varying Lipschitz Growth Rates.

IEEE Trans Cybern 2021 Sep 20;PP. Epub 2021 Sep 20.

Prescribed-time consensus tracking for second-order nonlinear multiagent systems (MASs) with the unknown nonlinear dynamics satisfying a time-varying Lipschitz growth rate is investigated in this article. A time-varying function is introduced as a part of the controller gains, and it plays an important role in overcoming the rapid growth of nonlinear terms and in ensuring that the consensus can be achieved in a preassigned time. An integral sliding-mode control protocol, which forces the system trajectory to move on to the defined sliding manifold at the initial moment, is proposed for solving the prescribed-time consensus tracking problem of leader-following MASs with disturbances. Furthermore, we propose a slightly different control law based on terminal sliding-mode control, and under such a controller, the trajectories of each follower reach the sliding manifold in an arbitrary assigned time T₁, and then in a specified time T₂, the position and velocity tracking errors for all followers converge to 0 at the same time instant. Based on the graph theory, state transformations, and Lyapunov theorem, we prove that the proposed solutions are feasible and, finally, three simulation examples are provided to verify the theoretical results.
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http://dx.doi.org/10.1109/TCYB.2021.3109294DOI Listing
September 2021
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