Publications by authors named "Yuqing Ren"

15 Publications

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Effects of andrographolide on renal tubulointersticial injury and fibrosis. Evidence of its mechanism of action.

Phytomedicine 2021 Oct 9;91:153650. Epub 2021 Jul 9.

Guangdong Pharmaceutical University, Guangzhou 510006, China; Guangdong Metabolic Diseases Research Center of Integrated Chinese and Western Medicine, Guangzhou 510006, China; Key Laboratory of Glucolipid Metabolic Disorder, Ministry of Education, Guangzhou 510006, China; Institute of Chinese Medicine, Guangdong TCM Key Laboratory for Metabolic Diseases, Guangzhou 510006, China. Electronic address:

Background: Diabetic nephropathy (DN) is associated with renal interstitial injury and fibrosis. Our previous study showed that andrographolide protected against the progression of DN and high glucose (HG)-induced mesangial dysfunction. However, the protective effects of andrographolide on renal tubular epithelial cells have not been fully elucidated.

Purpose: To determine the protective effects of andrographolide on renal tubular damage and explore the underlying mechanism.

Study Design: Human tubular epithelial cells (HK-2 cells) were treated with andrographolide (5 and 10 μM) under HG conditions. Diabetic mice were treated with andrographolide (i.p. 2 and 4 mg/kg, twice per week).

Methods: Western blotting, reverse transcription-polymerase chain reaction (RT-PCR), immunofluorescence and flow cytometry were used to analyze the effects of andrographolide on renal tubular injury and fibrotic mechanisms in HK-2 cells. The protective effects of andrographolide against renal tubulointerstitial injury and fibrosis were investigated in diabetic mice fed a high-fat diet (HFD). Renal interstitial tissue was collected at sacrifice for immunohistochemistry, immunofluorescence analysis, RT-PCR and Western blotting to analyze the effects of andrographolide on renal tubular injury and fibrosis.

Results: In vitro assay results indicated that andrographolide (5 and 10 μM) effectively inhibited HG-induced apoptosis, epithelial-mesenchymal transition (EMT) and collagen deposition in HK-2 cells. Mechanistically, HG stimulated mitochondrial reactive oxygen species (mtROS)-mediated NOD-like receptor family and pyrin domain-containing protein 3 (NLRP3) inflammasome activation and EMT in tubular epithelial cells, and andrographolide (5 and 10 μM) inhibited these effects by ameliorating mitochondrial dysfunction. In vivo, treatment with andrographolide (2 and 4 mg/kg) inhibited renal tubular cell apoptosis, EMT and tubulointerstitial fibrosis, mitochondrial dysfunction and NLRP3 inflammasome activation in diabetic mice.

Conclusion: Andrographolide (5 and 10 μM) prevents HG-induced renal tubular cell damage, and andrographolide (2 and 4 mg/kg) protects against the progression of diabetic tubular injury and fibrosis in mice by suppressing mitochondrial dysfunction and NLRP3 inflammasome activation.
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http://dx.doi.org/10.1016/j.phymed.2021.153650DOI Listing
October 2021

[Study on the safety and effectiveness of low-dose tranexamic acid in operation of multi-level continuous thoracic ossification of ligament flavum].

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2021 Jul;35(7):873-877

Department of Orthopedics, Affiliated Hospital of North Sichuan Medical College, Nanchong Sichuan, 637000, P.R.China.

Objective: To investigate the safety and effectiveness of low-dose tranexamic acid (TXA) in operation of multi-level continuous thoracic ossification of ligament flavum (TOLF).

Methods: A clinical data of 26 patients who underwent operation for multi-level continuous TOLF and met the selection criteria between July 2015 and January 2019 was retrospectively analyzed. Among them, 13 cases (group A) were received intravenous infusion of TXA (10 mg/kg) at 15 minutes before operation, and maintained the infusion at 1 mg/(kg·h) until the end of the operation; 13 cases (group B) were received the same dose of normal saline before and during operation. There was no significant difference in gender, age, body mass index, diseased segment, and preoperative hemoglobin, platelet count, activated partial thromboplastin time, prothrombin time, international normalized ratio (INR) between the two groups ( >0.05). The hemoglobin, platelet count, activated partial thromboplastin time, prothrombin time, INR, the number of deep vein thrombosis of the lower extremities, operation time, intraoperative blood loss, postoperative drainage volume, total blood loss, and the time of drainage tube extubation in the two groups were recorded and compared.

Results: All operations in the two groups were successfully completed. Compared with group B, the operation time and time of drainage tube extubation in group A were shortened, and the intraoperative blood loss, postoperative drainage volume, and total blood loss were reduced. The differences between the two groups were significant ( <0.05). None of the two groups received blood transfusion, and the hemoglobin level of group A at 24 hours after operation was significantly higher than that of group B ( =5.062, =0.000). The incisions in both groups healed and sutures were removed within 2 weeks after operation, and no complications occurred. There was no significant difference between the two groups in activated partial thromboplastin time, prothrombin time, INR, and platelet count at 24 hours after operation ( >0.05).

Conclusion: In multi-level continuous TOLF operation, intravenous administration of low-dose TXA can effectively reduce blood loss, shorten postoperative drainage time, and does not increase the risk of complications.
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http://dx.doi.org/10.7507/1002-1892.202101084DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8311222PMC
July 2021

Responses of microbial structures, functions and metabolic pathways for nitrogen removal to different hydraulic retention times in anaerobic side-stream reactor coupled membrane bioreactors.

Bioresour Technol 2021 Jun 25;329:124903. Epub 2021 Feb 25.

Shanghai Engineering Research Center of Energy - Saving in Heat Exchange Systems, Shanghai University of Electric Power, Shanghai 200090, China.

Synchronous sludge reduction and nitrogen removal have attracted increasing attention, while the underlying mechanisms of diverse nitrogen metabolism within the complicated processes remain unclear. Four anoxic/oxic membrane bioreactors, three of which were upgraded by anaerobic side-stream reactors (ASSR) and carriers (APSSR-MBRs), were operated to determine effects of hydraulic retention time of ASSRs. APSSR-MBRs achieved more significant nitrogen removal and higher nitrate uptake rate because of more denitrifying bacteria and the supernumerary release of secondary substrates. Ammonia uptake rate showed the diverse Nitrospira preceded over anaerobic decay and sulfide inhibition in the ASSR, and made the reactor exhibit higher nitrification capacity. Metagenomic analysis indicated that APSSR-MBRs showed higher abundances of genes related to nitrogen consumption processes, and higher abundances on the carriers, confirming their pivotal roles in nitrogen metabolism. This study provided novel perspectives to build a bridge between process model and nitrogen metabolism in the sludge reduction system..
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http://dx.doi.org/10.1016/j.biortech.2021.124903DOI Listing
June 2021

Inhibitors of α-amylase and α-glucosidase: Potential linkage for whole cereal foods on prevention of hyperglycemia.

Food Sci Nutr 2020 Dec 4;8(12):6320-6337. Epub 2020 Nov 4.

China-Canada Joint Lab of Food Nutrition and Health (Beijing) Beijing Engineering and Technology Research Center of Food Additives Beijing Technology & Business University (BTBU) Beijing China.

The strategy of reducing carbohydrate digestibility by controlling the activity of two hydrolyzing enzymes (α-amylase and α-glucosidase) to control postprandial hyperglycemia is considered as a viable prophylactic treatment of type 2 diabetes mellitus (T2DM). Thus, the consumption of foods rich in hydrolyzing enzyme inhibitors is recommended for diet therapy of diabetes. Whole cereal products have gained increasing interests for plasma glucose-reducing effects. However, the mechanisms for whole cereal benefits in relation to T2DM are not yet fully understood, but most likely involve bioactive components. Cereal-derived phenolic compounds, peptides, nonstarch polysaccharides, and lipids have been shown to inhibit α-amylase and α-glucosidase activities. These hydrolyzing enzyme inhibitors seem to make whole cereals become nutritional strategies in managing postmeal glucose for T2DM. This review presents an updated overview on the effects provided by cereal-derived ingredients on carbohydrate digestibility. It suggests that there is some evidence for whole cereal intake to be beneficial in amelioration of T2DM through inhibiting α-glucosidase and α-amylase activities.
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http://dx.doi.org/10.1002/fsn3.1987DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7723208PMC
December 2020

Calcium leaching characteristics in landfill leachate collection systems from bottom ash of municipal solid waste incineration.

J Environ Manage 2021 Feb 28;280:111729. Epub 2020 Nov 28.

School of Environment, Tsinghua University, Beijing, 100084, PR China. Electronic address:

Calcium leaching is a critical factor in the clogging of leachate collection systems (LCS), a phenomenon that affects landfill stability and operation. The bottom ash (BA) of municipal solid waste (MSW) incineration plants contains large quantities of calcium-based compounds. Landfilling is the main disposal method for BA in China that intensifies the consequences of LCS clogging. The factors influencing BA calcium leaching were investigated using simulated leachate. The results showed that fine BA particles, low pH values, high temperature, and ratios of leachate to BA solids were conducive to calcium leaching. Calcium leaching was found to be higher in actual leachate than in simulated leachate. At pH = 5, the cumulative calcium dissolution ratios (CDRs) were 83.36% and 31.49% after 20 days of leaching in the actual and simulated leachate, respectively; at pH = 6, the values were 50.67% and 12.06%, respectively. The introduction of landfill gas could decrease the calcium dissolution and leaching rates. When the ratio of leachate to BA solid was 20:1 mL/g, the accumulative CDR values were 45.98% (pH = 6) and 5.80% (pH = 8) without landfill gases, and 4.59% (pH = 6) and 0.48% (pH = 8) with landfill gases. These results provide the scientific basis for clogging risk prediction with respect to calcium leaching in the LCS of landfills. BA landfilling in old landfill areas with relatively high leachate pH and low chemical oxygen demand, as well as when leachate mixed with an appropriate amount of landfill gases, could be feasible measures to reduce calcium leaching and further prevent clogging in LCS.
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http://dx.doi.org/10.1016/j.jenvman.2020.111729DOI Listing
February 2021

Comprehensive analysis of the long non-coding RNA-associated competitive endogenous RNA network reveals novel prognostic biomarkers in Wilms' tumor.

Oncol Lett 2020 Jun 31;19(6):3731-3742. Epub 2020 Mar 31.

Department of Medical Oncology, The Sixth Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong 510655, P.R. China.

Wilms' tumor (WT) is one of the most common types of renal carcinoma in children. The aim of the present study was to construct a competitive endogenous RNA (ceRNA) regulation network and explore novel prognostic biomarkers for WT. The expression profiles were downloaded from The Cancer Genome Atlas database to identify differentially expressed RNAs (DERNAs). Based on the interactions between microRNAs (miRNAs) and mRNAs/long non-coding RNAs (lncRNAs), a ceRNA network was constructed. Functional enrichment analyses were subsequently conducted to explore the functions of the ceRNA-associated DEmRNAs. Survival analysis was performed to screen for prognosis-associated RNAs and the χ test was used to assess the associations between prognosis-associated RNA expression and histology classification/clinical staging. The present study identified 1,784 lncRNAs, 114 miRNAs and 3,337 mRNAs, which were abnormally expressed in WT compared with that in normal samples. By prediction, pairing and network analysis, a ceRNA network consisting of 38 DElncRNAs, 18 DEmiRNAs and 99 DEmRNAs was established. These DEmRNAs were significantly enriched in pathways associated with the occurrence and development of WT. By combining the expression data with survival analysis, seven prognosis-associated RNAs were identified (P<0.05). Of these seven RNAs, two (zinc finger and BTB domain containing 4; and deleted in lymphocytic leukemia 2) were significantly associated with clinical staging and histology classification. Lastly, the expression levels of the seven RNAs were verified in the Gene Expression Omnibus database. The present study revealed that 7 RNAs might be considered as novel prognostic biomarkers and potential treatment targets for therapy in WT. In addition, the ceRNA regulation network could provide novel strategies for further studies on lncRNAs and miRNAs in WT.
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http://dx.doi.org/10.3892/ol.2020.11500DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7202313PMC
June 2020

Dynamics of ammonia oxidizers and denitrifiers in response to compost addition in black soil, Northeast China.

PeerJ 2020 21;8:e8844. Epub 2020 Apr 21.

College of Resources and Environment, Northeast Agricultural University, Harbin, Heilongjiang, China.

Organic fertilizer application could have an impact on the nitrogen cycle mediated by microorganisms in arable soils. However, the dynamics of soil ammonia oxidizers and denitrifiers in response to compost addition are less understood. In this study, we examined the effect of four compost application rates (0, 11.25, 22.5 and 45 t/ha) on soil ammonia oxidizers and denitrifiers at soybean seedling, flowering and mature stage in a field experiment in Northeast China. As revealed by quantitative PCR, compost addition significantly enhanced the abundance of ammonia oxidizing bacteria (AOB) at seedling stage, while the abundance of ammonia oxidizing archaea was unaffected across the growing season. The abundance of genes involved in denitrification (, and ) were generally increased along with compost rate at seedling and flowering stages, but not in mature stage. The non-metric multidimensional scaling analysis revealed that moderate and high level of compost addition consistently induced shift in AOB and containing denitrifers community composition across the growing season. Among AOB lineages, cluster 3a gradually decreased along with the compost rate across the growing season, while exhibited an opposite trend. Network analysis indicated that the complexity of AOB and containing denitrifiers network gradually increased along with the compost rate. Our findings highlighted the positive effect of compost addition on the abundance of ammonia oxidizers and denitrifiers and emphasized that compost addition play crucial roles in shaping their community compositions and co-occurrence networks in black soil of Northeast China.
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http://dx.doi.org/10.7717/peerj.8844DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7182023PMC
April 2020

Phycocyanin Exerts Anti-Proliferative Effects through Down-Regulating TIRAP/NF-κB Activity in Human Non-Small Cell Lung Cancer Cells.

Cells 2019 06 14;8(6). Epub 2019 Jun 14.

Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, Beijing 100048, China.

Phycocyanin is a type of marine functional food additive, exerting a health care efficacy with no side effects. It has been shown that phycocyanin possesses anticancer function in non-small cell lung cancer (NSCLC) cells, but the underlying regulatory mechanism still remains unclear. Further investigation on the antineoplastic mechanism of phycocyanin would provide useful information on NSCLC treatment. In this study, we explored the in vitro function and mechanism of phycocyanin in three typical NSCLC cell lines, H1975, H1650, and LTEP-a2, for the first time. Phenotypic experiments showed that phycocyanin significantly induced the apoptosis as well as suppressed the growth of NSCLC cells. Transcriptome analysis suggested that toll/interleukin 1 receptor domain-containing adaptor protein (TIRAP) was significantly down-regulated by phycocyanin. Strikingly, similar to phycocyanin-treated assays, siRNA knockdown of TIRAP expression also resulted in the anti-proliferative phenomenon in NSCLC cells. In addition, the activity of NF-κB signaling was also suppressed after silencing TIRAP expression, revealing that phycocyanin exerted anti-proliferative function through down-regulating TIRAP/NF-κB activity in NSCLC cells. Collectively, this study has laid a theoretical basis on the treatment of NSCLC and the potential utilization of marine functional products.
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http://dx.doi.org/10.3390/cells8060588DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6627414PMC
June 2019

Exploring the mechanisms of organic matter degradation and methane emission during sewage sludge composting with added vesuvianite: Insights into the prediction of microbial metabolic function and enzymatic activity.

Bioresour Technol 2019 Aug 30;286:121397. Epub 2019 Apr 30.

School of Environment, Henan Key Laboratory for Environmental Pollution Control, Key Laboratory for Yellow River and Huai River Water Environmental Pollution Control, Ministry of Education, Henan Normal University, Xinxiang, Henan 453007, PR China.

Effect mechanisms of organic matter (OM) degradation and methane (CH) emission during sewage sludge (SS) composting with added vesuvianite (V) were studied by high-throughput sequencing (HTS) and phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt). Results show that the addition of V accelerated the OM degradation and decreased the cumulative CH emissions by 33.6% relative to the control. In addition, V significantly decreased the mcrA gene abundance and the methanogen community richness at the genus level. PICRUSt also indicated that V strengthens the microbial metabolic function and enzymatic activity related to OM degradation, and reduced the enzymatic activity related to CH production. Methanogens community variation analysis proved the ratio of carbon and nitrogen and moisture content are the significant variables affecting CH emissions. Thus, optimizing the ratio of carbon and nitrogen and moisture content will decrease CH emission during SS composting.
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http://dx.doi.org/10.1016/j.biortech.2019.121397DOI Listing
August 2019

Beneficial influences of pelelith and dicyandiamide on gaseous emissions and the fungal community during sewage sludge composting.

Environ Sci Pollut Res Int 2019 Mar 4;26(9):8928-8938. Epub 2019 Feb 4.

School of Environment, Key Laboratory for Yellow River and Huai River Water Environmental and Pollution Control, Ministry of Education, Henan Key Laboratory for Environmental Pollution Control, Henan Normal University, Xinxiang, 453007, Henan, China.

Reducing the emissions of NH and greenhouse gases (GHGs) during composting is essential for improving compost quality and controlling environmental pollution. This paper investigates the effects of pelelith (P) combined with dicyandiamide (DCD) on gaseous emissions and the fungal community diversity during sewage sludge (SS) composting. Results showed that the P and P + DCD treatments decreased the cumulative gaseous emissions by 41% and 22% for NH, 21% and 34% for NO, and 31.5% and 33.0% for CH, respectively. The evolution of the fungal community analysis showed that Ascomycota and unclassified fungi dominated during the thermophilic stage, while only Ascomycota was the dominant fungal phylum during the maturity stage, composing 62%, 66%, and 73% of the total fungal community in the control, P, and P + DCD, respectively. The P and P + DCD significantly increased the fungal community richness at the genus level. Fungal community abundance was found to be significantly related to temperature, pH, organic matter, and total Kjeldahl nitrogen, which also influence the gaseous emissions during SS composting. It suggested that the combined addition of pelelith and dicyandiamide (DCD) was an effective method for reducing the emissions of NH and greenhouse gases during SS composting.
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http://dx.doi.org/10.1007/s11356-019-04404-xDOI Listing
March 2019

Insight Investigation of Active Palladium Surface Sites in Palladium-Ceria Catalysts for NO + CO Reaction.

ACS Appl Mater Interfaces 2018 Apr 12;10(16):13614-13624. Epub 2018 Apr 12.

Key Laboratory for Special Fuctional Aggregate Materials of Education Ministry, School of Chemistry and Chemical Engineering , Shandong University , Jinan 250100 , P. R. China.

The palladium species in ceria-based catalysts have a significant influence on their catalytic performance. In this work, the structure evolution of palladium species induced by various calcination rate was investigated and then these calcined catalysts were applied to NO + CO catalytic reaction. Systematic investigations by various measurements demonstrate that the calcination rate and catalytic process play crucial roles on the formation ways of palladium species and identify the forms of active palladium surface sites for NO + CO reaction. Results indicate that the calcination process resulted in the formation of three types of palladium components: PdO interacted with ceria supports (PdO /Pd-O-Ce cluster), PdO nanoparticles on the surface, and Pd ions incorporated into the subsurface lattice (Pd-O-Ce solid solution). It is also proven that the state and distribution of palladium components are dependent on the calcination rate: rapid calcination rate is beneficial for the generation of PdO species (PdO /Pd-O-Ce and PdO), while slow calcination rate makes contribution to the formation of Pd-O-Ce. Furthermore, the subsequent catalytic process could induce the decomposition of PdO /Pd-O-Ce and formation of more fractions of active Pd species in PdO oxide phase. On the basis of the catalytic performances, we found the superior catalytic properties are detected for catalysts containing 0.5% Pd (0.5% is corresponding to the palladium content in molar ratio) with fast calcination rate. This is due to the synergistic effect of active Pd in PdO decomposed form PdO /Pd-O-Ce in the catalytic process and the palladium ions in Pd-O-Ce solid solution.
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http://dx.doi.org/10.1021/acsami.8b02557DOI Listing
April 2018

Bioaccessibility and health risk assessment of arsenic in soil and indoor dust in rural and urban areas of Hubei province, China.

Ecotoxicol Environ Saf 2016 Apr 17;126:14-22. Epub 2015 Dec 17.

State Key Laboratory of Water Environment Simulation, School of Environment, Beijing Normal University, Beijing 100875, China. Electronic address:

Incidental oral ingestion is the main exposure pathway by which human intake contaminants in both soil and indoor dust, and this is especially true for children as they frequently exhibit hand-to-mouth behaviour. Research on comprehensive health risk caused by incidental ingestion of both soil and indoor dust is limited. The aims of this study were to investigate the arsenic concentration and to characterize the health risks due to arsenic (As) exposure via soil and indoor dust in rural and urban areas of Hubei province within central China. Soil and indoor dust samples were collected from schools and residential locations and bioaccessibility of arsenic in these samples was determined by a simplified bioaccessibility extraction test (SBET). The total arsenic content in indoor dust samples was 1.78-2.60 times that measured in soil samples. The mean As bioaccessibility ranged from 75.4% to 83.2% in indoor dust samples and from 13.8% to 20.2% in soil samples. A Pearson's analysis showed that As bioaccessibility was significantly correlated with Fe and Al in soil and indoor dust, respectively, and activity patterns of children were utilised in the assessment of health risk via incidental ingestion of soil and indoor dust. The results suggest no non-carcinogenic health risks (HQ<1) or acceptable carcinogenic health risks (1×10(-6)
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http://dx.doi.org/10.1016/j.ecoenv.2015.11.037DOI Listing
April 2016

Characterization, treatment and releases of PBDEs and PAHs in a typical municipal sewage treatment plant situated beside an urban river, East China.

J Environ Sci (China) 2013 Jul;25(7):1281-90

State Key Laboratory of Water Environment Simulation, School of Environment, Beijing Normal University, Beijing 100875, China

Characterization, treatment and releases of eight polybrominated diphenyl ethers (PBDEs) congeners and sixteen polycyclic aromatic hydrocarbons (PAHs) in wastewater were evaluated along the treatment processes of a typical secondary treatment municipal sewage treatment plant (STP) (in Hefei City) situated the beside Nanfei River, East China. The findings showed that the average concentrations of the total PBDEs in raw wastewater and treated effluent were 188.578 and 36.884 ng/L respectively. Brominated diphenyl ether (BDE) 209 congener, the predominant PBDE in the STP and Nanfei River, could be related to the discharge of car-industry-derived wastes. For PAHs, the average concentrations in raw wastewater and treated effluent were 5758.8 and 2240.4 ng/L respectively, with naphthalene, benzo[a]pyrene and indeno[1,2,3-c,d]pyrene being detected at the highest concentrations. PAHs mainly originate from the combustion of biomass/coal and petroleum. The STP reduced about 80% of the PBDEs and 61% of the PAHs, which were eliminated mainly by sedimentation processes. The removal rates of PBDEs/PAHs increased with the increase of their solid-water partitioning coefficients. Accordingly, the STP's effluent, containing some PBDE congeners (e.g., BDE 47, 99 and 209, etc.) and low-molecular-weight PAHs, could be an important contributor of these contaminants' input to Nanfei River. It resulted in a significant increase of PBDE/PAH concentrations and PAH toxicological risk in the river water downstream. About 4.040 kg/yr of PBDEs and 245.324 kg/yr of PAHs could be released into the Nanfei River. The current conventional wastewater treatment processes should be improved to remove the relatively low-molecular-weight PBDEs/PAHs more effectively.
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http://dx.doi.org/10.1016/s1001-0742(12)60201-0DOI Listing
July 2013

Polybrominated diphenyl ethers occurrence in major inflowing rivers of Lake Chaohu (China): characteristics, potential sources and inputs to lake.

Chemosphere 2013 Nov 8;93(8):1624-31. Epub 2013 Sep 8.

State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China.

Eight commonly occurring polybrominated diphenyl ethers (PBDEs), including BDE 28, 47, 99, 100, 153, 154, 183, 207, and 209, were investigated in water samples from seven major inflowing rivers of Lake Chaohu to determine the distribution characteristics, potential sources and inputs to the lake. The sum of 8 BDE congeners (Σ8PBDEs) had a concentration varied from 0.31 to 84 ng L(-1), with those of BDE 209, BDE 47, BDE 99, and BDE 153 being 0.31-83, <0.012-0.36, <0.012-1.3, and <0.012-0.77 ng L(-1), respectively. These levels were in the high range of the global PBDEs concentrations in the water environments. The highest concentrations of Σ8PBDEs were detected in the western rivers, of which the main pollution sources were strongly related to human activities in urban centers, such as automobile-derived wastes. A sewage treatment plant was likely an important source of the lower brominated BDEs input to one western river. The correlation analyses (all p<0.05) between PBDEs and DOC, TN, TP, and EC, suggested that the distributions and sources of PBDEs in rivers might also be related with the soil erosion by heave floods. Σ8PBDEs input to Lake Chaohu from the rivers outlets was estimated at 344 kg yr(-1) during the flood season. BDE 209 was the dominant contributor with an input of 340 kg yr(-1), followed by BDE 99 (1.3 kg yr(-1)), BDE 47 (0.83 kg yr(-1)) and BDE 153 (0.60 kg yr(-1)).
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http://dx.doi.org/10.1016/j.chemosphere.2013.08.024DOI Listing
November 2013

Twist overexpression promoted epithelial-to-mesenchymal transition of human peritoneal mesothelial cells under high glucose.

Nephrol Dial Transplant 2012 Nov 11;27(11):4119-24. Epub 2012 Apr 11.

Department of Nephrology, Yangquan Coalmine Group General Hospital, Shanxi, China.

Background: Long-term peritoneal dialysis (PD) results in functional and structural alterations of the peritoneal membrane. Previous studies have suggested that high glucose (HG) could induce transdifferentiation of peritoneal mesothelial cells into myofibroblasts, but the molecular mechanisms of HG-induced epithelial-to-mesenchymal transition (EMT) of human peritoneal mesothelial cells (HPMCs) are unclear. This study was undertaken to elucidate the effects and mechanisms of Twist on HG-induced EMT of HPMCs.

Methods: HPMCs were exposed to 5.6 mM glucose [normal glucose (NG)], 50 mM glucose (HG) or 50 mM glucose with Si-Twist or pcDNA3.1-Twist. Western blot and immuocytochemistry were performed to determine Twist, E-cadherin and α-smooth muscle actin (α-SMA) protein expression. MMP2 and MMP9 were detected by zymography. Rats were daily instilled with PD fluid and lipopolysaccharide (LPS) or sodium chloride during 6 weeks. Histological analyses were carried out in parietal peritoneum. Twist was detected by western blotting.

Results: Twist and α-SMA protein and immuocytochemistry were significantly increased in HG-conditioned media compared to NG media. E-cadherin protein was lower in pcDNA3.1-Twist-transfected HPMCs compared to pcDNA3.1 cells. Twist protein was upregulated 12 h after HG stimulation. MMP9 was increased in pcDNA3.1-Twist-transfected HPMCs compared to pcDNA3.1 cells. Exposure of rat peritoneum to PD fluid and LPS resulted in an increase of extracellular matrix deposition. Twist and α-SMA were stained in the PD fluid group and compared to the control group. Twist protein was significantly increased in the PD group.

Conclusions: In conclusion, HG-induced Twist expression might contribute to EMT of HPMCs. Twist may control EMT of HPMCs by regulating MMP9.
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http://dx.doi.org/10.1093/ndt/gfs049DOI Listing
November 2012
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