Publications by authors named "Yuqing Huang"

122 Publications

Optimization of twin parallel microstrips based nuclear magnetic resonance probe for measuring the kinetics in molecular assembly in ultra-small samples.

Rev Sci Instrum 2021 Mar;92(3):033106

Department of Electronic Science, Fujian Provincial Key Laboratory of Plasma and Magnetic Resonance, State Key Laboratory for Physical Chemistry of Solid Surfaces, Xiamen University, Xiamen 361005, People's Republic of China.

We present the design, fabrication, characterization, and optimization of a TPM (twin parallel microstrip)-based nuclear magnetic resonance (NMR) probe, produced by using a low-loss Teflon PTFE F4B high frequency circuit board. We use finite element analysis to optimize the radio frequency (RF) homogeneity and sensitivity of the TPM probe jointly for various sample volumes. The RF homogeneity of this TPM planar probe is superior to that of only a single microstrip probe. The optimized TPM probe properties such as RF homogeneity and field strength are characterized experimentally and discussed in detail. By combining this TPM based NMR probe with microfluidic technology, the sample amount required for kinetic study using NMR spectroscopy was minimized. This is important for studying costly samples. The TPM NMR probes provide high sensitivity to analysis of 5 µl samples with 2 mM concentrations within 10 min. The miniaturized microfluidic NMR probe plays an important role in realizing down to seconds timescale for kinetic monitoring.
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http://dx.doi.org/10.1063/5.0030452DOI Listing
March 2021

Comparison of 1064-nm and Dual-Wavelength (532/1064-nm) Picosecond-Domain Nd:YAG Lasers in the Treatment of Facial Photoaging: A Randomized Controlled Split-Face Study.

Lasers Surg Med 2021 Mar 30. Epub 2021 Mar 30.

Department of Dermatologic Surgery, Hospital of Dermatology, Peking Union Medical College (PUMC), Chinese Academy of Medical Sciences (CAMS), Jiangwangmiao Street 12, Xuanwu District, Nanjing, Jiangsu, 210042, China.

Background And Objectives: To compare the efficacy and safety of 1064-nm and dual-wavelength (532/1064 nm) picosecond Nd:YAG lasers with a holographic optic in the treatment of facial photoaging.

Study Design/materials And Methods: In this prospective, randomized split-face study, each half of each participant's face was randomly treated with a 1064-nm or 532/1064-nm picosecond laser. All participants underwent five treatment sessions and follow-up visits 1 and 3 months after the final treatment. The primary outcomes were measured as the global photoaging scores, determined by two physicians who were blinded to the treatments. The secondary outcomes were the participant-assessed Global Aesthetic Improvement Scale (GAIS) and satisfaction scores. Histopathological examinations were performed.

Results: Global photoaging scores decreased significantly after treatment with either laser. The global photoaging scores, GAIS scores, and satisfaction scores did not differ significantly between the 1064-nm and 532/1064-nm picosecond laser treatments. Histological changes were similar between the two groups. Intraepidermal vacuoles and dermal hemorrhaging were observed immediately and 24 h after treatment. After five treatments, neocollagenesis was observed in the upper dermis of both groups, and elastic fibers were more elongated and orderly.

Conclusions: Treatments with 1064-nm and 532/1064-nm picosecond Nd:YAG lasers were comparably effective at improving photodamaged facial skin by remodeling the collagen and elastin network through laser-induced optical degradation and vascular damage.
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http://dx.doi.org/10.1002/lsm.23404DOI Listing
March 2021

Printed Stretchable Liquid Metal Electrode Arrays for In Vivo Neural Recording.

Small 2021 Apr 12;17(14):e2006612. Epub 2021 Mar 12.

School of Life Science and Technology, Harbin Institute of Technology, 2 Yikuang Road, Nangang District, Harbin, 150001, P. R. China.

The adoption of neural interfacing into neurological diagnosis is severely hampered by the complex, costly, and error-prone manufacturing methods, requiring new fabrication processes and materials for flexible neural interfacing. Here a strategy for fabricating highly stretchable neural electrode arrays based on screen printing of liquid metal conductors onto polydimethylsiloxane substrates is presented. The screen-printed electrode arrays show a resolution of 50 µm, which is ideally applicable to neural interfaces. The integration of liquid metal-polymer conductor enables the neural electrode arrays to retain stable electrical properties and compliant mechanical performance under a significant (≈108%) strain. Taking advantage of its high biocompatibility, liquid metal electrode arrays exhibit excellent performance for neurite growth and long-term implantation. The stretchable electrode arrays can spontaneously conformally come in touch with the brain surface, and high-throughput electrocorticogram signals are recorded. Based on stretchable electrode arrays, real-time monitoring of epileptiform activities can be provided at different states of seizure. The method reported here offers a new fabrication strategy to manufacture stretchable neural electrodes, with additional potential utility in diagnostic brain-machine interfaces.
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http://dx.doi.org/10.1002/smll.202006612DOI Listing
April 2021

Impact Analysis of miR-1253 on Lung Cancer Progression Through Targeted Regulation of ANXA3.

Cancer Manag Res 2021 19;13:1767-1776. Epub 2021 Feb 19.

Department of Thoracic Surgery, Peking University People's Hospital, Beijing, 100044, People's Republic of China.

Objective: This study set out to investigate the effect of on lung cancer progression through targeted regulation of .

Methods: RT-PCR was employed to detect the expression levels in lung cancer cells and its targeted gene mRNA determined by biological information prediction. MTT, invasion and apoptosis rate tests were employed to detect the proliferation, invasion and apoptosis rate of lung cancer cells over-expressing or those with low expression of and the expression of related proteins.

Results: RT-qPCR results manifested that the level was down-regulated in lung cancer tissues and cells, and the expression increased. The and expression levels were negatively correlated. was correlated with tumor differentiation degree, TNM stage and lymph node metastasis of lung cancer patients. Cell tests confirmed that played a tumor-inhibiting function, including inhibiting proliferation and invasion of lung cancer cells and promoting apoptosis. Bioinformatics prediction and subsequent experiments proved that was the direct target of . Moreover, after the expression in lung cancer cells was knocked down, proliferation and invasion of those cells were inhibited dramatically, the apoptosis rate increased markedly, and the expression levels of pro-apoptosis-related proteins and were up-regulated, and the anti-apoptosis-related protein expression was down-regulated.

Conclusion: can inhibit the proliferation and invasion of lung cancer cells and promote their apoptosis by targeting . It can be used as a new potential target for lung cancer treatment.
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http://dx.doi.org/10.2147/CMAR.S251679DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7903947PMC
February 2021

Development and Validation of Machine Learning-based Model for the Prediction of Malignancy in Multiple Pulmonary Nodules: Analysis from Multicentric Cohorts.

Clin Cancer Res 2021 Feb 24. Epub 2021 Feb 24.

Department of Thoracic Surgery, Peking University People's Hospital, Beijing, China.

Purpose: Nodule evaluation is challenging and critical to diagnose multiple pulmonary nodules (MPNs). We aimed to develop and validate a machine learning-based model to estimate the malignant probability of MPNs to guide decision-making.

Experimental Design: A boosted ensemble algorithm (XGBoost) was used to predict malignancy using the clinicoradiologic variables of 1,739 nodules from 520 patients with MPNs at a Chinese center. The model (PKU-M model) was trained using 10-fold cross-validation in which hyperparameters were selected and fine-tuned. The model was validated and compared with solitary pulmonary nodule (SPN) models, clinicians, and a computer-aided diagnosis (CADx) system in an independent transnational cohort and a prospective multicentric cohort.

Results: The PKU-M model showed excellent discrimination [area under the curve; AUC (95% confidence interval (95% CI)), 0.909 (0.854-0.946)] and calibration (Brier score, 0.122) in the development cohort. External validation (583 nodules) revealed that the AUC of the PKU-M model was 0.890 (0.859-0.916), higher than those of the Brock model [0.806 (0.771-0.838)], PKU model [0.780 (0.743-0.817)], Mayo model [0.739 (0.697-0.776)], and VA model [0.682 (0.640-0.722)]. Prospective comparison (200 nodules) showed that the AUC of the PKU-M model [0.871 (0.815-0.915)] was higher than that of surgeons [0.790 (0.711-0.852), 0.741 (0.662-0.804), and 0.727 (0.650-0.788)], radiologist [0.748 (0.671-0.814)], and the CADx system [0.757 (0.682-0.818)]. Furthermore, the model outperformed the clinicians with an increase of 14.3% in sensitivity and 7.8% in specificity.

Conclusions: After its development using machine learning algorithms, validation using transnational multicentric cohorts, and prospective comparison with clinicians and the CADx system, this novel prediction model for MPNs presented solid performance as a convenient reference to help decision-making.
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http://dx.doi.org/10.1158/1078-0432.CCR-20-4007DOI Listing
February 2021

Histological Characteristics of Skin Treated With a Fractionated 1064-nm Nd: YAG Picosecond Laser With Holographic Optics.

Lasers Surg Med 2021 Feb 10. Epub 2021 Feb 10.

Department of Cosmetic Laser Surgery, Hospital for Skin Disease, Institute of Dermatology, Peking Union Medical College (PUMC), Chinese Academy of Medical Sciences (CAMS), Jiangwangmiao Street 12, Xuanwu District, Nanjing, Jiangsu, 210042, China.

Background And Objectives: Picosecond lasers (PSL) constitute a significant technological advancement and exert rejuvenating effects upon the skin. This study was conducted to investigate changes in the skin upon treatment with the fractionated 1064-nm Nd: YAG PSL through in vivo and ex vivo human histological analysis.

Study Design/materials And Methods: In vivo back skin specimens were treated with a fractionated 1064-nm PSL at 1.3, 2.1, and 2.9 mJ fluence for two passes, and 2.9 mJ for 10 passes, and then stained with hematoxylin and eosin (H&E). Ex vivo foreskin specimens after circumcision surgery were treated with a PSL at 1.3, 2.1, and 2.9 mJ fluence for two and 10 passes, followed by H&E staining. Ex vivo skin tissue sections treated with a PSL at 2.9 mJ fluence for 10 passes were also immunostained for Melan-A and CD31.

Results: Intraepidermal vacuoles were observed, along with pigment accumulation and inflammatory cell infiltration in the vacuoles at 24 hours after PSL treatment in the in vivo skin specimens. The vacuoles expanded as the fluence increased. Numerous intraepidermal vacuoles were observed, with dermal hemorrhage and inflammatory cell infiltration upon high-fluence, multi-pass PSL treatment in the in vivo skin specimens. PSL treatment yielded both epidermal and dermal vacuoles in ex vivo skin specimens. Melan-A-positive cells were seen in the cystic wall of vacuoles in the epidermal basal layer, whereas CD31-positive cells were detected in the cystic wall of some dermal vacuoles.

Conclusions: The fractionated 1064-nm PSL produced epidermal vacuoles and dermal lesions, with histological differences between the in vivo and ex vivo skin specimens. Lasers Surg. Med. © 2021 Wiley Periodicals LLC.
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http://dx.doi.org/10.1002/lsm.23389DOI Listing
February 2021

Diffusion Analysis on Complex Mixtures under Adverse Magnetic Field Conditions by Spatially-Selective Pure Shift-Based DOSY.

J Phys Chem Lett 2021 Jan 20;12(3):1073-1080. Epub 2021 Jan 20.

Department of Electronic Science, Fujian Provincial Key Laboratory of Plasma and Magnetic Resonance, State Key Laboratory of Physical Chemistry of Solid Surfaces, Xiamen University, Xiamen, China.

Diffusion-ordered NMR spectroscopy (DOSY) serves as a noninvasive spectroscopic method for studying intact mixtures and identifying individual components present in mixtures according to their diffusion behaviors. However, DOSY techniques generally fail to discriminate complex compositions which exhibit crowded or overlapped NMR signals, particularly under adverse magnetic field conditions. Herein, we exploit the spatially selective pure shift-based DOSY strategy to address this challenge by eliminating inhomogeneous line broadenings and extracting pure shift singlets, thereby expediting diffusion analyses on complex mixtures. More importantly, this strategy is further applied to observing and analyzing electro-oxidation processes of blended alcohols, suggesting its potential to monitoring electrochemical reactions. This study demonstrates a meaningful NMR trial for diffusion analysis on complex mixtures under adverse experimental circumstances, and particularly, it provides a proof-of-concept technique for electrochemical studies and shows promising prospects for applications in chemistry, biology, energy, etc.
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http://dx.doi.org/10.1021/acs.jpclett.0c03549DOI Listing
January 2021

Highly Efficient Determination of Complex NMR Multiplet Structures in Inhomogeneous Magnetic Fields.

Anal Chem 2021 02 4;93(4):2419-2423. Epub 2021 Jan 4.

Department of Electronic Science, Fujian Provincial Key Laboratory of Plasma and Magnetic Resonance, State Key Laboratory of Physical Chemistry of Solid Surfaces, Xiamen University, Siming South Road 422, Xiamen, China.

Proton-proton scalar () coupling plays an important role in disentangling molecular structures and spatial conformations. But it is challenging to extract coupling networks from congested H NMR spectra, especially in inhomogeneous magnetic fields. Herein, we propose a general liquid NMR protocol, named HR-G-SERF, to implement highly efficient determination of individual couplings and corresponding coupling networks via simultaneously suppressing effects of spectral congestions and magnetic field inhomogeneity. This method records full-resolved 2D absorption-mode spectra to deliver great convenience for multipet analyses on complex samples. More meaningfully, it is capable of disentangling multiplet structures of biological samples, that is, grape sarcocarp, despite of its heterogeneous semisolid state and extensive compositions. In addition, a modification, named AH-G-SERF, is developed to compress experimental acquisition and subsequently improve unit-time SNR, while maintaining satisfactory spectral performance. This accelerated variant may further boost the applicability for rapid NMR detections and afford the possibility of adopting hyperpolarized substances to enhance the overall sensitivity. Therefore, this study provides a promising tool for molecular structure elucidations and composition analyses in chemistry, biochemistry, and metabonomics among others.
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http://dx.doi.org/10.1021/acs.analchem.0c04365DOI Listing
February 2021

Prevalence of and risk factors for abnormal left ventricular geometrical patterns in hypertensive subjects administered irbesartan.

J Clin Lab Anal 2021 Mar 2;35(3):e23688. Epub 2021 Jan 2.

Provincial Key Laboratory of Coronary Heart Disease Prevention, Department of Cardiology, Guangdong Cardiovascular institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China.

Background: Distinct populations differ in LVH prevalence and impaired LV geometry. Currently, the prevalence of and risk factors for LV geometric patterns in Chinese hypertensives administered irbesartan have not been specifically addressed in large studies.

Methods: Totally 10,883 patients (6623 men and 4260 women) completed the survey, including 1181 hypertensives administered irbesartan (488 males and 693 females) that were finally enrolled. Based on LVMI and RWT derived from comprehensive echocardiography, the LV geometric patterns of irbesartan-treated hypertensive individuals were classified into four types, including the normal, concentric remodeling, and concentric and eccentric hypertrophy groups. Logistic regression analysis was applied in males and females, respectively, for determining odds ratios (ORs) and 95% confidence intervals (CIs) for various potential risk factors for abnormal LV geometrical patterns in irbesartan-treated hypertensives.

Results: The clinical and echocardiographic data differed significantly between males and females. The prevalence rates of concentric remodeling, concentric hypertrophy, and eccentric hypertrophy were 36.3%, 15.4%, and 6.1% in males, respectively, and 23.5%, 20.3%, and 23.8% in females, accordingly. Gender, daily dose of irbesartan, BMI, SBP, WtHR, and neck-circumference were significantly associated with LV geometric patterns. After adjustment for confounding factors, risk factors for LVH and impaired LV geometry included SBP, WtHR in males, and MAU-Cr and WtHR in females.

Conclusions: LVH and impaired LV geometric patterns are more prevalent in females (67.7%) compared with that in males (57.8%) among hypertensives upon irbesartan administration. For such population, risk factors beyond elevated blood pressure may be involved in the progression of LVH and impaired LV geometric patterns in both genders.
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http://dx.doi.org/10.1002/jcla.23688DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7957976PMC
March 2021

OsPPR939, a nad5 splicing factor, is essential for plant growth and pollen development in rice.

Theor Appl Genet 2021 Mar 2;134(3):923-940. Epub 2021 Jan 2.

Institute of Crop Science, Zhejiang University, Hangzhou, 310058, China.

Key Message: P-subfamily PPR protein OsPPR939, which can be phosphorylated by OsS6K1, regulates plant growth and pollen development by involving in the splicing of mitochondrial nad5 introns 1, 2, and 3. In land plants, pentatricopeptide repeat (PPR) proteins play key roles in mitochondrial group II intron splicing, but how these nucleus-encoded proteins are imported into mitochondria is unknown. To date, a few PPR proteins have been characterized in rice (Oryza sativa). Here, we demonstrate that the mitochondrion-localized P-subfamily PPR protein OsPPR939 is required for the splicing of nad5 introns 1, 2, and 3 in rice. Complete knockout or partial disruption of OsPPR939 function resulted in different degrees of growth retardation and pollen sterility. The dramatically reduced splicing efficiency of these introns in osppr939-4 and osppr939-5 led to reduced mitochondrial complex I abundance and activity and enhanced expression of alternative respiratory pathway genes. Complementation with OsPPR939 rescued the defective plant morphology of osppr939-4 and restored its decreased splicing efficiency of nad5 introns 1, 2, and 3. Therefore, OsPPR939 plays crucial roles in plant growth and pollen development by splicing mitochondrial nad5 introns 1, 2, and 3. More importantly, the 12th amino acid Ser in the N-terminal targeting sequence of OsPPR939 is phosphorylated by OsS6K1, and truncated OsPPR939 with a non-phosphorylatable S12A mutation in its presequence could not be imported into mitochondria, suggesting that phosphorylation of this amino acid plays an important role in the mitochondrial import of OsPPR939. To our knowledge, the 12th residue Ser on OsPPR939 is the first experimentally proven phosphorylation site in PPR proteins. Our results provide a basis for investigating the regulatory mechanism of PPR proteins at the post-translational level.
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http://dx.doi.org/10.1007/s00122-020-03742-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7925476PMC
March 2021

siMTA1-Loaded Exosomes Enhanced Chemotherapeutic Effect of Gemcitabine in Luminal-b Type Breast Cancer by Inhibition of EMT/HIF-α and Autophagy Pathways.

Front Oncol 2020 13;10:541262. Epub 2020 Nov 13.

Department of Gastrointestinal Surgery, Department of General Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, China.

Advanced breast cancer holds a poor prognosis for chemotherapy and endocrine therapy resistance. Autophagy is one of the main causes of tumor drug-therapy failure, and increasing evidence shows that EMT also is responsible for that. Metastasis-associated protein1 (MTA1) is up regulated in lots of tumors, which leads to tumor progression and drug resistance. However, the role of MTA1 in chemotherapeutic resistance in luminal-b breast cancer is still unclear. In this paper, our research shows that higher expression of MTA1 accompanies with worse prognosis in luminal-b breast cancer. Knockdown of MTA1 enhances the sensitivity of MCF-7 to gemcitabine and weakens the metastasis ability of MCF-7 and . Further, we find that knockdown of MTA1 strengthens the gemcitabine-mediated tumor growth inhibition effect , through reversion of the EMT process and inhibition of the autophagy process. Furthermore, our research builds the siMTA1-loaded exosomes, which increases the gemcitabine-mediated tumor growth inhibition effect .
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http://dx.doi.org/10.3389/fonc.2020.541262DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7691572PMC
November 2020

Evolution of rapid blue-light response linked to explosive diversification of ferns in angiosperm forests.

New Phytol 2021 05 7;230(3):1201-1213. Epub 2021 Jan 7.

School of Science, Western Sydney University, Penrith, NSW, 2751, Australia.

Ferns appear in the fossil record some 200 Myr before angiosperms. However, as angiosperm-dominated forest canopies emerged in the Cretaceous period there was an explosive diversification of modern (leptosporangiate) ferns, which thrived in low, blue-enhanced light beneath angiosperm canopies. A mechanistic explanation for this transformative event in the diversification of ferns has remained elusive. We used physiological assays, transcriptome analysis and evolutionary bioinformatics to investigate a potential connection between the evolution of enhanced stomatal sensitivity to blue light in modern ferns and the rise of angiosperm-dominated forests in the geological record. We demonstrate that members of the largest subclade of leptosporangiate ferns, Polypodiales, have significantly faster stomatal response to blue light than more ancient fern lineages and a representative angiosperm. We link this higher sensitivity to levels of differentially expressed genes in blue-light signaling, particularly in the cryptochrome (CRY) signaling pathway. Moreover, CRYs of the Polypodiales examined show gene duplication events between 212.9-196.9 and 164.4-151.8 Ma, when angiosperms were emerging, which are lacking in other major clades of extant land plants. These findings suggest that evolution of stomatal blue-light sensitivity helped modern ferns exploit the shady habitat beneath angiosperm forest canopies, fueling their Cretaceous hyperdiversification.
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http://dx.doi.org/10.1111/nph.17135DOI Listing
May 2021

MYSM1 Suppresses Cellular Senescence and the Aging Process to Prolong Lifespan.

Adv Sci (Weinh) 2020 Nov 30;7(22):2001950. Epub 2020 Sep 30.

State Key Laboratory of Virology College of Life Sciences Wuhan University Wuhan 430072 China.

Aging is a universal feature of life that is a major focus of scientific research and a risk factor in many diseases. A comprehensive understanding of the cellular and molecular mechanisms of aging are critical to the prevention of diseases associated with the aging process. Here, it is shown that MYSM1 is a key suppressor of aging and aging-related pathologies. MYSM1 functionally represses cellular senescence and the aging process in human and mice primary cells and in mice organs. MYSM1 mechanistically attenuates the aging process by promoting DNA repair processes. Remarkably, MYSM1 deficiency facilitates the aging process and reduces lifespan, whereas MYSM1 over-expression attenuates the aging process and increases lifespan in mice. The functional role of MYSM1 is demonstrated in suppressing the aging process and prolonging lifespan. MYSM1 is a key suppressor of aging and may act as a potential agent for the prevention of aging and aging-associated diseases.
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http://dx.doi.org/10.1002/advs.202001950DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7675055PMC
November 2020

3D-printed integrative probeheads for magnetic resonance.

Nat Commun 2020 11 13;11(1):5793. Epub 2020 Nov 13.

Department of Electronic Science, Fujian Provincial Key Laboratory of Plasma and Magnetic Resonance, Xiamen University, 361005, Xiamen, China.

Magnetic resonance (MR) technology has been widely employed in scientific research, clinical diagnosis and geological survey. However, the fabrication of MR radio frequency probeheads still face difficulties in integration, customization and miniaturization. Here, we utilized 3D printing and liquid metal filling techniques to fabricate integrative radio frequency probeheads for MR experiments. The 3D-printed probehead with micrometer precision generally consists of liquid metal coils, customized sample chambers and radio frequency circuit interfaces. We screened different 3D printing materials and optimized the liquid metals by incorporating metal microparticles. The 3D-printed probeheads are capable of performing both routine and nonconventional MR experiments, including in situ electrochemical analysis, in situ reaction monitoring with continues-flow paramagnetic particles and ions separation, and small-sample MR imaging. Due to the flexibility and accuracy of 3D printing techniques, we can accurately obtain complicated coil geometries at the micrometer scale, shortening the fabrication timescale and extending the application scenarios.
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http://dx.doi.org/10.1038/s41467-020-19711-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7666178PMC
November 2020

Reference-frame-independent measurement-device-independent quantum key distribution with mismatched-basis statistics.

Opt Lett 2020 Nov;45(22):6334-6337

The source flaw associated with the basis vector in the reference-frame-independent measurement-device-independent quantum key distribution (RFI-MDI-QKD) has not been systematically studied. As a result, it is often assumed that bit error is equal to phase error, which is not theoretically rigorous. Here, we propose a postprocessing method to estimate the phase error rate from the discarded mismatched-basis statistics, where the qubit source does not need to be characterized in detail. The source flaw in the basis vector of the RFI-MDI-QKD protocol can thus be corrected using this method. The numerical simulation results clearly demonstrate that the RFI-MDI-QKD protocol with uncharacterized sources is also insensitive to the misalignment of the reference frame.
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http://dx.doi.org/10.1364/OL.403481DOI Listing
November 2020

Transient circular dichroism and exciton spin dynamics in all-inorganic halide perovskites.

Nat Commun 2020 Nov 9;11(1):5665. Epub 2020 Nov 9.

Division of Physics and Applied Physics, School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore, 637371, Singapore.

All-inorganic metal halides perovskites (CsPbX, X = Br or Cl) show strong excitonic and spin-orbital coupling effects, underpinning spin-selective excitonic transitions and therefore exhibiting great promise for spintronics and quantum-optics applications. Here we report spin-dependent optical nonlinearities in CsPbX single crystals by using ultrafast pump-probe spectroscopy. Many-body interactions between spin-polarized excitons act like a pseudo-magnetic field and thus lift the degeneracy of spin states resulting in a photoinduced circular dichroism. Such spontaneous spin splitting between "spin-up" and "spin-down" excitons can be several tens of milli-electron volts under intense excitations. The exciton spin relaxation time is ~20 picoseconds at very low pump fluence, the longest reported in the metal halides perovskites family at room temperature. The dominant spin-flip mechanism is attributed to the electron-hole exchange interactions. Our results provide essential understandings towards realizing practical spintronics applications of perovskite semiconductors.
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http://dx.doi.org/10.1038/s41467-020-19471-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7653957PMC
November 2020

Improving efficiency of measuring individual H coupling networks by pure shift 2D J-resolved NMR spectroscopy.

J Chem Phys 2020 Nov;153(17):174114

Department of Electronic Science, Fujian Provincial Key Laboratory of Plasma and Magnetic Resonance, State Key Laboratory for Physical Chemistry of Solid Surfaces, Xiamen University, Xiamen 361005, China.

The H coupling networks, including H-H correlation and J coupling values, provide the important information for structure elucidation and conformation analysis. However, the presence of a large number of couplings and the phase-twist lineshapes often prevents revealing H coupling networks. Here, we provide a clean absorption-mode 2D NMR method, SIMAJ (SImple Methods for 2D Absorption mode J-resolved spectrum), for a straightforward assignment and measurement of the coupling network involving the chosen proton. Relying on the pure shift element, H-H couplings and chemical shift evolution are totally separately demonstrating along the F and F dimensions, respectively. Processing with a single experiment dataset and free of 45° spectral shearing, an absorption-mode 2D J-resolved spectrum can be reconstructed. Two pulse sequences were proposed as examples. The SIMAJ signal processing method will be a general procedure for obtaining absorption-mode lineshapes when analyzing the experiment datasets with chemical shifts and J coupling multiplets in the orthogonal dimensions. With excellent sensitivity, high spectral purity, and ability of easily identifying H-H correlations, significant improvements are beneficial for structural, conformational, or complex composition analyses.
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http://dx.doi.org/10.1063/5.0025962DOI Listing
November 2020

Functional Self-Assembled DNA Nanohydrogels for Specific Telomerase Activity Imaging and Telomerase-Activated Antitumor Gene Therapy.

Anal Chem 2020 11 28;92(22):15179-15186. Epub 2020 Oct 28.

MOE Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, State Key Laboratory of Photocatalysis on Energy and Environment, College of Chemistry, Fuzhou University, Fuzhou 350116, People's Republic of China.

Engineering a functional nanoplatform that integrates dynamic monitoring of endogenous biomarkers and a stimuli-activated therapeutic mode is promising for early diagnosis and treatment of cancers. In this study, we developed an intelligent DNA nanohydrogel with specific targeting capability that can be stimuli-activated for both in vitro telomerase detection and in vivo telomerase-triggered gene therapy. The DNA nanohydrogel was formed simply by the self-assembly of two Y-shaped DNA units and a double-stranded DNA linker labeled with fluorophores and loaded with therapeutic siRNA. When intracellular telomerase was overexpressed, the DNA nanohydrogel collapsed owing to the prolongation of the telomeric primer at the terminal sequence of one of the Y-shaped DNA units. As a result, the quenched fluorescence due to fluorescence resonance energy transfer (FRET) of the DNA nanohydrogel recovered and the trapped siRNA was released, enabling the accurate detection and imaging of intracellular telomerase activity as well as effective gene therapy of tumors. Benefiting from the great biocompatibility, specificity, and stimuli-responsive property, the developed DNA nanoplatform provides a new opportunity for precise cancer diagnosis and treatment as well as other biological applications.
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http://dx.doi.org/10.1021/acs.analchem.0c03746DOI Listing
November 2020

HIV-1 Nef Interacts with LMP7 To Attenuate Immunoproteasome Formation and Major Histocompatibility Complex Class I Antigen Presentation.

mBio 2020 10 27;11(5). Epub 2020 Oct 27.

State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, China

The proteasome is a major protein degradation machinery with essential and diverse biological functions. Upon induction by cytokines, proteasome subunits β1, β2, and β5 are replaced by β1i/LMP2, β2i/MECL-1, and β5i/LMP7, resulting in the formation of an immunoproteasome (iProteasome). iProteasome-degraded products are loaded onto the major histocompatibility complex class I (MHC-I), regulating immune responses and inducing cytotoxic T lymphocytes (CTLs). Human immunodeficiency virus type 1 (HIV-1) is the causal agent of AIDS. HIV-1-specific CTLs represent a critical immune mechanism limiting viral replication. HIV-1 negative regulatory factor (Nef) counteracts host immunity, particularly the response involving MHC-I/CTL. This study identifies a distinct mechanism by which Nef facilitates immune evasion via suppressing the function of iProteasome and MHC-I. Nef interacts with LMP7 on the endoplasmic reticulum (ER), downregulating the incorporation of LMP7 into iProteasome and thereby attenuating its formation. Moreover, Nef represses the iProteasome function of protein degradation, MHC-I trafficking, and antigen presentation. The ubiquitin-proteasome system (UPS) is essential for the degradation of damaged proteins, which takes place in the proteasome. Upon activation by cytokines, the catalytic subunits of the proteasome are replaced by distinct isoforms resulting in the formation of an immunoproteasome (iProteasome). iProteasome generates peptides used by major histocompatibility complex class I (MHC-I) for antigen presentation and is essential for immune responses. HIV-1 is the causative agent of AIDS, and HIV-1-specific cytotoxic T lymphocytes (CTLs) provide immune responses limiting viral replication. This study identifies a distinct mechanism by which HIV-1 promotes immune evasion. The viral protein negative regulatory factor (Nef) interacts with a component of iProteasome, LMP7, attenuating iProteasome formation and protein degradation function, and thus repressing the MHC-I antigen presentation activity of MHC-I. Therefore, HIV-1 targets LMP7 to inhibit iProteasome activation, and LMP7 may be used as the target for the development of anti-HIV-1/AIDS therapy.
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http://dx.doi.org/10.1128/mBio.02221-19DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7593969PMC
October 2020

MYSM1 Represses Innate Immunity and Autoimmunity through Suppressing the cGAS-STING Pathway.

Cell Rep 2020 10;33(3):108297

State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China; Guangzhou Provincial Key Laboratory of Virology, Institute of Medical Microbiology, Jinan University, Guangzhou 510632, China. Electronic address:

The immune system is not only required for preventing threats exerted by pathogens but also essential for developing immune tolerance to avoid tissue damage. This study identifies a distinct mechanism by which MYSM1 suppresses innate immunity and autoimmunity. The expression of MYSM1 is induced upon DNA virus infection and by intracellular DNA stimulation. MYSM1 subsequently interacts with STING and cleaves STING K63-linked ubiquitination to suppress cGAS-STING signaling. Notably, Mysm1-deficient mice exhibit a hyper-inflammatory response, acute tissue damage, and high mortality upon virus infection. Moreover, in the PBMCs of patients with systemic lupus erythematosus (SLE), MYSM1 production decreases, while type I interferons and pro-inflammatory cytokine expressions increase. Importantly, MYSM1 treatment represses the production of IFNs and pro-inflammatory cytokines in the PBMCs of SLE patients. Thus, MYSM1 is a critical repressor of innate immunity and autoimmunity and is thus a potential therapeutic agent for infectious, inflammatory, and autoimmune diseases.
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http://dx.doi.org/10.1016/j.celrep.2020.108297DOI Listing
October 2020

Transcriptome-Based Analysis of Phosphite-Induced Resistance Against Pathogens in Rice.

Plants (Basel) 2020 Oct 9;9(10). Epub 2020 Oct 9.

Institute of Crop Science, Hangzhou Academy of Agricultural Sciences, Hangzhou 310000, China.

Phosphite (PHI) has been used in the management of diseases since the 1970s.We assessed the effect of PHI on controlling the incidence of and . As a result, PHI application significantly inhibited the incidence of the diseases. To clarify the molecular mechanism underlying this, a transcriptome study was employed. In total, 2064 differentially expressed genes (DEGs) were identified between control and PHI treatment. The key DEGs could be classified into phenylpropanoid biosynthesis (ko00940), starch and sucrose metabolism (ko00500), and plant hormone signal transduction (ko04075). The expressions of defense-related genes had a higher expression lever upon PHI treatment. This study provides new insights into the mechanism of protection effect of PHI against pathogens.
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http://dx.doi.org/10.3390/plants9101334DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7650589PMC
October 2020

Cyanidin-3-O-glucoside attenuates high glucose-induced podocytes dysfunction by inhibiting apoptosis and promoting autophagy via activation of SIRT1/AMPK pathway.

Can J Physiol Pharmacol 2020 Oct 13. Epub 2020 Oct 13.

The Affiliated Hospital of Southwest Medical University, 556508, Luzhou, Sichuan, China;

Diabetic nephropathy (DN) is a common and complicated chronic kidney disease around the world. To elucidate and find effective therapies of DN is of vital importance. In this paper, we have discovered that Cyanidin-3-O-glucoside (C3G), which is one of the anthocyanins, could alleviate high glucose induced podocytes dysfunction. MTT, flow cytometry assay and western blot analysis showed that C3G could reverse the increase of cell apoptosis under high glucose treatment in MPC5 cells by up-regulation of Bcl2 and down-regulation of Bax and cleaved-caspase 3. Moreover, C3G improved the autophagy decrease that was induced by high glucose through regulating the expression level of LC3 II/LC3 I, Beclin1 and p62. In addition, C3G inhibited epithelial-mesenchymal transition (EMT) by increasing E-cadherin and reducing Vimentin. By further mechanisms study, we found C3G activated the SIRT1 and AMPK which were inhibited in high glucose condition. Silencing SIRT1 blocked the effect of C3G on regulating cell apoptosis, autophagy and EMT. In summary, our current findings suggest the protective effect of C3G against high glucose induced podocytes dysfunction is by improving autophagy and reducing apoptosis and EMT via activating SIRT1/AMPK pathway. It might be a new insight for the treatment of DN.
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http://dx.doi.org/10.1139/cjpp-2020-0341DOI Listing
October 2020

Characterization and Identification of PpEIN3 during the Modulation of Fruit Ripening Process by Ectopic Expressions in Tomato.

Plant Genome 2019 11;12(3):1-12

College of Horticulture, Nanjing Agricultural Univ., Nanjing, 210095, China.

Core Ideas: First study of PpEIN3 by transgenic experiments to verify its function in the maturity process PpEIN3 is a positive regulator of ethylene signal transduction pathway to promote fruits ripening Ethylene is one of the most important phytohormone in plants and plays a critical role during growth, development, maturity, and aging. The framework of the ethylene signaling pathway is well reported. Nevertheless, studies on Ethylene Insensitive 3 (EIN3), the downstream regulator of the ethylene signaling pathway, need to be investigated, especially in peach [Prunus persica (L.) Batsch]. In this study, we cloned PpEIN3 from peach and characterized it in tomato (Solanum lycopersicum L.). Our results depicted that the open-reading frame of PpEIN3 was 1875 bp, encoding a protein with 624 amino acid residues that contained a conserved EIN3 domain, a highly conserved N-terminal region, and seven DNA-binding sites. PpEIN3 showed very close association with homologous EIN genes from apple (Malus domestica Borkh.) and grapevine (Vitis vinifera L.). All investigated EIN proteins shared similar domains and structures. The PpEIN3 promoter possessed several motifs related to hormones that affect fruit development and ripening. Spatial-temporal expression analysis revealed that PpEIN3 was expressed at high levels in the late stage of fruit development vs. the early stage. In transgenic tomato, PpEIIN3 showed overexpression and the key ethylene biosynthesis genes SlACO1, SlACS1, and SlSAMS1 were upregulated and promoted early maturation in fruit. By contrast, PpEIIN3 silencing delayed ripening and reduced SlEIN3 expression in tomato. The results confirmed that PpEIN3 is a positive regulator of the ethylene signal transduction pathway, which promoted fruit ripening. Our findings provide valuable insight to the roles in ethylene signal components in the modulation of peach fruit ripening.
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http://dx.doi.org/10.3835/plantgenome2018.11.0089DOI Listing
November 2019

Detection and Analysis of C-to-U RNA Editing in Rice Mitochondria-Encoded ORFs.

Plants (Basel) 2020 Sep 28;9(10). Epub 2020 Sep 28.

Zhejiang Provincial Key Laboratory of Crop Germplasm Resources, Institute of Crop Science, Zhejiang University, No. 866, Yu-Hang-Tang Road, Hangzhou 310058, China.

Cytidine to uridine (C-to-U) RNA editing is an important type of substitutional RNA modification and is almost omnipresent in plant chloroplasts and mitochondria. In rice mitochondria, 491 C-to-U editing sites have been identified previously, and case studies have elucidated the function of several C-to-U editing sites in rice, but the functional consequence of most C-to-U alterations needs to be investigated further. Here, by means of Sanger sequencing and publicly available RNA-seq data, we identified a total of 569 C-to-U editing sites in rice mitochondria-encoded open reading frames (ORFs), 85.41% of these editing sites were observed on the first or the second base of a codon, resulting in the alteration of encoded amino acid. Moreover, we found some novel editing sites and several inaccurately annotated sites which may be functionally important, based on the highly conserved amino acids encoded by these edited codons. Finally, we annotated all 569 C-to-U RNA editing sites in their biological context. More precise information about C-to-U editing sites in rice mitochondria-encoded ORFs will facilitate our investigation on the function of C-to-U editing events in rice and also provide a valid benchmark from rice for the analysis of mitochondria C-to-U editing in other plant species.
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http://dx.doi.org/10.3390/plants9101277DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7600565PMC
September 2020

A Study of Two Cases Co-Infected with SARS-CoV-2 and Human Immunodeficiency Virus.

Virol Sin 2020 12 7;35(6):849-852. Epub 2020 Sep 7.

State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, 430000, China.

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http://dx.doi.org/10.1007/s12250-020-00280-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7475944PMC
December 2020

RalA exerts an inhibitory effect on IL-1β/IL-18 secretion by blocking NLRP3 inflammasome activation in levornidazole-treated human THP-1 macrophages.

Int Immunopharmacol 2020 Nov 28;88:106898. Epub 2020 Aug 28.

Department of Cell Biology, Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, 209 Tongshan Road, Xuzhou 221009, Jiangsu, China. Electronic address:

The NLRP3 inflammasome is an important mediator of inflammatory responses and its regulation is an active area of research. RalA is a Ras-like GTPase, which play pivotal roles in the biology of cells. So far, there have been very few studies on RalA regulating inflammatory responses. Bioinformatics analysis predicted that RalA might participate in the regulatory network of NLRP3 inflammasome, which has been confirmed in THP-1 macrophages. After virtual screening of compounds, it was found that levonidazole selected from our virtual small molecule compound library has the potential to bind to RalA. Of note, the interaction of RalA/levornidazole was verified by Surface Plasmon Resonance-Biacore T200, LC/MS analysis and Western blotting analysis. Molecular dynamics simulations revealed that the conformational changes of RalA might be regulated by levornidazole. Additionally, IL-1β/IL-18 secretion from ATP + LPS stimulated THP-1-derived macrophages was RalA-dependently suppressed by levornidazole, suggesting that RalA might have an inhibitory effect on NLRP3 inflammasome activation. The results of co-immunoprecipitation and RalA depletion experiments showed that levornidazole could induce RalA to block the assembly of NLRP3/ASC/pro-caspase-1 complex, thereby reducing the levels of cleaved-caspase-1 and IL-1β/IL-18 secretion. Our study has suggested an anti-inflammatory function of RalA and identified its targeting chemical compound. Overall, this study clarifies a novel pharmacological mechanism by which RalA/levornidazole inhibits NLRP3 inflammasome activation and IL-1β/IL-18 secretion.
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http://dx.doi.org/10.1016/j.intimp.2020.106898DOI Listing
November 2020

Digitoxin inhibits proliferation of multidrug-resistant HepG2 cells through G/M cell cycle arrest and apoptosis.

Oncol Lett 2020 Oct 30;20(4):71. Epub 2020 Jul 30.

Formula Pattern Research Center, School of Traditional Chinese Medicine, Jinan University, Guangzhou, Guangdong 510632, P.R. China.

Hepatocellular carcinoma (HCC) remains a challenge in the medical field due to its high malignancy and mortality rates particularly for HCC, which has developed multidrug resistance. Therefore, the identification of efficient chemotherapeutic drugs for multidrug resistant HCC has become an urgent issue. Natural products have always been of significance in drug discovery. In the present study, a cell-based method was used to screen a natural compound library, which consisted of 78 compounds, and the doxorubicin-resistant cancer cell line, HepG2/ADM, as screening tools. The findings of the present study led to the shortlisting of one of the compounds, digitoxin, which displayed an inhibitory effect on HepG2/ADM cells, with 50% inhibitory concentration values of 132.65±3.83, 52.29±6.26, and 9.13±3.67 nM for 24, 48, and 72 h, respectively. Immunofluorescence, western blotting and cell cycle analyses revealed that digitoxin induced G/M cell cycle arrest via the serine/threonine-protein kinase ATR (ATR)-serine/threonine-protein kinase Chk2 (CHK2)-M-phase inducer phosphatase 3 (CDC25C) signaling pathway in HepG2/ADM cells, which may have resulted from a DNA double-stranded break. Digitoxin also induced mitochondrial apoptosis, which was characterized by changes in the interaction between Bcl-2 and Bax, the release of cytochrome , as well as the activation of the caspase-3 and -9. To the best of our knowledge, the present study is the first report that digitoxin displays an anti-HCC effect on HepG2/ADM cells through G/M cell cycle arrest, which was mediated by the ATR-CHK2-CDC25C signaling pathway and mitochondrial apoptosis. Therefore, digitoxin could be a promising chemotherapeutic agent for the treatment of patients with HCC.
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http://dx.doi.org/10.3892/ol.2020.11932DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7436926PMC
October 2020

Highly efficient photothermal conversion capric acid phase change microcapsule: Silicon carbide modified melamine urea formaldehyde.

J Colloid Interface Sci 2021 Jan 5;582(Pt A):30-40. Epub 2020 Aug 5.

School of Civil Engineering and Architecture, Nanchang University, Nanchang, Jiangxi 330031, China.

The microcapsule containing phase change materials(microPCMs) with high efficiency of photothermal conversion was prepared by in-situ polymerization via ultrasonic dispersion which used capric acid(CA) as core material and nano silicon carbide(nano-SiC) modified melamine-urea-formaldehyde(MUF) resin as wall material. The nano-SiC has good cross-linking with MUF shell. When the nano-SiC was added in microPCMs, it behaves superior thermal conductivity and thermal storage properties. When the content of nano-SiC arrives 6 wt%, the performance of the microPCMs whose encapsulation efficiency is 65.7% is the best, and thermal conductivity increase by 59.2%. Due to the proper amount of nano-SiC added into the MUF shell, it can effectively fill the tiny holes on the MUF shell. Therefore, the microPCMs with appropriate nano-SiC have better leakage prevention. It is worth noting that MicroPCMs-6% and MicroPCMs-8% show excellent photothermal conversion property, and the photothermal conversion rate is 74.4% and 71.1% respectively in the photothermal conversion experiment. Because nano-SiC can effectively capture and absorb photons under light irradiation and convert light into heat through internal molecular vibration, the microPCMs with appropriate nano-SiC behaves well in photothermal conversion. In other words, microPCMs have potential in solar energy utilization and thermal energy storage.
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http://dx.doi.org/10.1016/j.jcis.2020.08.014DOI Listing
January 2021

Unusual metachronous lung adenocarcinomas harboring EGFR L858R/T790M mutations: A case report.

Thorac Cancer 2020 10 12;11(10):3020-3023. Epub 2020 Aug 12.

Department of Thoracic Surgery, Beijing Haidian Hospital, Haidian Section of Peking University Third Hospital, Beijing, China.

Multiple primary lung cancer (MPLC) is defined as two or more primary lung cancers occurring in the same patient and can be classified as synchronous multiple primary lung cancer (sMPLC) and metachronous multiple primary lung cancer (mMPLC). Due to various clinicopathological characteristics and genetic features, MPLC is increasingly encountered in clinical practice. The distinction between MPLC and intrapulmonary metastasis (IM) is of great importance to clinical treatment and prognosis. However, there are currently no golden diagnostic criteria for MPLC due to tumor heterogeneity. Here, we report the case of a patient with four lung cancers (tumor 1, named T1, in the right middle lobe seven years earlier; tumor 2, named T2, in the left lower lobe; tumor 3 and tumor 4, named T3 and T4, in the left upper lobe) and two tumors (T1 and T2) which shared the mutation in epidermal growth factor receptor (EGFR) L858R/T790M based on targeted multigene sequencing, which indicate that these two tumors might have originated from a common ancestor. However, based on previously published guidelines, these three tumors (T2T4) were diagnosed as mMPLC.
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http://dx.doi.org/10.1111/1759-7714.13618DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7529550PMC
October 2020

Roseotoxin B alleviates cholestatic liver fibrosis through inhibiting PDGF-B/PDGFR-β pathway in hepatic stellate cells.

Cell Death Dis 2020 06 15;11(6):458. Epub 2020 Jun 15.

Department of Cell Biology, Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, 209 Tongshan Road, Xuzhou, 221009, Jiangsu, China.

Identifying effective anti-fibrotic therapies is a major clinical need that remains unmet. In the present study, roseotoxin B was shown to possess an improving effect on cholestatic liver fibrosis in bile duct-ligated mice, as proved by histochemical and immunohistochemical staining, hepatic biochemical parameters, and TUNEL apoptotic cell detection in tissue sections. Using cellular thermal shift assay, computational molecular docking, microscale thermophoresis technology, and surface plasmon resonance biosensor, we confirmed that PDGFR-β was a direct target of roseotoxin B in fibrotic livers. Of note, human tissue microarrays detected pathologically high expression of p-PDGFR-β in liver samples of ~80% of patients with liver fibrosis and cirrhosis. PDGF-B/PDGFR-β pathway promotes transdifferentiation and excessive proliferation of hepatic stellate cells (HSCs), which is a very crucial driver for liver fibrosis. Meaningfully, roseotoxin B blocked the formation of PDGF-BB/PDGFR-ββ complex by targeting the D2 domain of PDGFR-β, thereby inhibiting the PDGF-B/PDGFR-β pathway in HSCs. In summary, our study provided roseotoxin B as a unique candidate agent for the treatment of liver fibrosis.
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http://dx.doi.org/10.1038/s41419-020-2575-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7296008PMC
June 2020