Publications by authors named "Yuqian Li"

143 Publications

Exposure to legacy and novel perfluoroalkyl substance disturbs the metabolic homeostasis in pregnant women and fetuses: A metabolome-wide association study.

Environ Int 2021 May 13;156:106627. Epub 2021 May 13.

State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, People's Republic of China.

Background: Perfluoroalkyl substances (PFASs) exist extensively and several of these have been verified to be toxic. Prenatal exposure to PFASs has attracted much attention. Metabolome-wide association analyses can be used to explore the toxicity mechanisms of PFASs by identifying associated biomarkers.

Objectives: To evaluate associations between the metabolites in maternal and cord serum and internal exposure to several common PFASs.

Methods: Paired maternal and cord serum samples were collected from 84 pregnant women who gave birth between 2015 and 2016. Seven legacy and two novel PFASs were measured. A nontarget metabolomic method and an iterative metabolite annotation based on metabolic pathways were applied to characterize the metabolic profiles. Linear regression adjusted with the false discovery rate and covariates was used to indicate the associations.

Results: A total of 279 features in maternal serum and 338 features in cord serum were identified as metabolites associated with PFAS exposure. Perfluorooctanoic acid (PFOA) and perfluorohexane sulfonic acid (PFHxS) were two PFASs associated with more metabolites, while the two novel chlorinated polyfluorinated ether sulfonic acids (Cl-PFESAs) showed less relevance to the metabolome. With pathway enrichment analysis, we found that three fatty acid metabolisms and retinol metabolism were correlated with PFAS exposure in maternal blood, and that sterol metabolism showed the correlation in both maternal serum and cord serum.

Conclusions: We identified metabolites and pathways in pregnant women and fetuses associated with the exposure to several PFAS, indicating a promising application for metabolome-wide association studies. Additional research is needed to confirm causation.
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http://dx.doi.org/10.1016/j.envint.2021.106627DOI Listing
May 2021

[Association between different types of obesity and blood pressure among adolescents in eastern China].

Wei Sheng Yan Jiu 2021 Mar;50(2):210-216

National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China.

Objective: To explore the association between different types of obesity and blood pressure in adolescents aged 12-17 years in eastern China.

Methods: Using multi-stage stratified random sampling, a total of 8279 adolescents aged between 12 and 17 years in eastern China were selected as the research objects from the "Nutrition and Health Surveillance for Chinese Children and Nursing Mothers" project from 2016 to 2017. According to BMI and WHtR, the subjects were divided into four types: non-obesity, general obesity, simple abdominal obesity and combined obesity. The normal high blood pressure and high blood pressure of people with different types of obesity were determined according to Blood Pressure Standards for Children at 3-17 Years Old Per Year and Height in China. The relationship between different types of obesity and the prevalence of high blood pressure was analyzed by χ~2 test, analysis of variance, multi-level linear model and multi-level Logistic regression model.

Results: The prevalence of high normal blood pressure was 13. 66%(1069), and the prevalence of high blood pressure was 18. 79%(1782) in adolescents aged 12 to 17 years in eastern China. In the non-obese group, the prevalence of high normal blood pressure was 12. 85%(732), and the prevalence of high blood pressure was 13. 79%(999). In the general obesity group, the prevalence of high normal blood pressure was 20. 45%(99), and the prevalence of high blood pressure was 23. 62%(160). In the central obesity group, the prevalence of high normal blood pressure was 6. 95%(32), and the prevalence of high blood pressure was 14. 64%(87). The prevalence of high normal blood pressure was 16. 68%(206), and the prevalence of high blood pressure was 42. 42%(536) in the complex obesity group. The prevalence of high blood pressure(χ~2=8. 05, P<0. 01) the difference was statistically significant. Results of the multi-level model showed that the risk of high blood pressure in central obesity group was significantly higher in girls than in non-obese group(OR=1. 50, 95%CI 1. 12-2. 02). The risk of high normal blood pressure(OR=2. 05, 95%CI 1. 62-2. 58; OR=1. 83, 95%CI 1. 38-2. 42) and high blood pressure(OR=2. 06, 95%CI 1. 59-2. 67; OR=1. 57, 95%CI 1. 15-2. 14) in obese boys and girls were significantly higher than those in non-obese group. The risk of high normal blood pressure(OR=3. 80, 95%CI 3. 19-4. 51; OR=2. 79, 95%CI 2. 30-3. 37), high blood pressure(OR=4. 07, 95%CI 3. 39-4. 88; OR=2. 84, 95%CI 2. 32-3. 46) in both boys and girls with compound obesity was significantly higher than that in the non-obese group.

Conclusion: Different types of obesity have varying degrees of correction with different blood pressure levels in adolescents aged 12-17 years, combined obesity has the highest risk of elevated blood pressure.
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http://dx.doi.org/10.19813/j.cnki.weishengyanjiu.2021.02.008DOI Listing
March 2021

[Association between glucose and blood lipid among adolescents in eastern China].

Wei Sheng Yan Jiu 2021 Mar;50(2):205-209

Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China.

Objective: To analyze the relationship between glucose and blood lipid among adolescents aged 12-17 years in eastern China during 2016-2017.

Methods: The data comes from the "Chinese Children and Nursing Mothers Nutrition and Health Monitoring" during 2016-2017. The detection of blood glucose and blood lipids were conducted among 8873 subjects(4405 boys, 4468 girls) from 8 provinces and 3 municipality of eastern China by multi-stage stratified cluster sampling method. Logistic regression was used to explore the factors related to abnormal blood glucose.

Results: The average fasting blood-glucose of boys was(5. 24±0. 53)mmol/L, and girls was(5. 13±0. 54)mmol/L(t=9. 64, P<0. 05). The average fasting blood-glucose decreased with the increased of age(F=109. 50, P<0. 05). The abnormal rate of fasting blood-glucose differed among the subjects of various age groups were significant difference(χ~2=27. 16, P<0. 05). The abnormal rate fasting blood glucose of boys was 3. 44%(149 cases), and girls was 1. 93%(85 cases)(χ~2=14. 06, P<0. 05). The abnormal rate of 12-13 years old was the higest(n=126, 3. 58%) and the 16-17 years old was the lowest(n=42, 1. 67%). Both the TC and LDL-C were higher among the subjects with abnormal fasting blood glucose than those with normal fasting blood glucose(t=3. 03, P<0. 05;t=2. 41, P<0. 05). The result of logistic analysis showed that as the increase of TC level, the risk of abnormal fasting blood glucose was increased(OR=1. 98, 95%CI 1. 06-3. 71).

Conclusion: The level of fasting blood glucose and the abnormal rate of fasting blood glucose varies in age and sex. The higher level of TC might be the risk factor of abnormal fasting blood glucose among adolescents.
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http://dx.doi.org/10.19813/j.cnki.weishengyanjiu.2021.02.007DOI Listing
March 2021

Associations of midpoint of sleep and night sleep duration with type 2 diabetes mellitus in Chinese rural population: the Henan rural cohort study.

BMC Public Health 2021 05 7;21(1):879. Epub 2021 May 7.

Department of Epidemiology and Biostatistics, College of Public Health, Zhengzhou University, 100 Kexue Avenue, Zhengzhou, 450001, Henan, PR China.

Background: The study aimed to investigate the independent and combined effects of midpoint of sleep and night sleep duration on type 2 diabetes mellitus (T2DM) in areas with limited resources.

Methods: A total of 37,276 participants (14,456 men and 22,820 women) were derived from the Henan Rural Cohort Study. Sleep information was assessed based on the Pittsburgh Sleep Quality Index. Logistic regression models and restricted cubic splines were used to estimate the relationship of the midpoint of sleep and night sleep duration with T2DM.

Results: Of the 37,276 included participants, 3580 subjects suffered from T2DM. The mean midpoint of sleep among the Early, Intermediate and Late groups were 1:05 AM ±23 min, 1:56 AM ±14 min, and 2:57 AM ±34 min, respectively. Compared to the Intermediate group, adjusted odds ratios (ORs) and 95% confidence interval (CI) of T2DM were 1.13 (1.04-1.22) and 1.14 (1.03-1.26) in the Early group and the Late group. Adjusted OR (95% CI) for T2DM compared with the reference (7- h) was 1.28 (1.08-1.51) for longer (≥ 10 h) night sleep duration. The combination of late midpoint of sleep and night sleep duration (≥ 9 h) increased 38% (95% CI 10-74%) prevalence of T2DM. These associations were more obvious in women than men.

Conclusions: Late and early midpoint of sleep and long night sleep duration were all associated with higher prevalence of T2DM. Meanwhile, midpoint of sleep and night sleep duration might have combined effects on the prevalence of T2DM, which provided potential health implications for T2DM prevention, especially in rural women.

Trial Registration: The Henan Rural Cohort Study has been registered at Chinese Clinical Trial Register (Registration number: ChiCTR-OOC-15006699 ). Date of registration: 2015-07-06.
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http://dx.doi.org/10.1186/s12889-021-10833-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8106181PMC
May 2021

Associations of spicy food flavour and intake frequency with blood lipid levels and risk of abnormal serum lipid levels in Chinese rural population: a cross-sectional study.

Public Health Nutr 2021 Apr 29:1-10. Epub 2021 Apr 29.

Department of Epidemiology and Biostatistics, College of Public Health, Zhengzhou University, 100 Kexue Avenue, Zhengzhou, HA450001, People's Republic of China.

Objective: The purpose of the current study was to investigate associations between spicy food intake and serum lipids levels in Chinese rural population.

Design: Information on spicy food flavour and intake frequency was obtained using a two-item questionnaire survey. Dietary data were collected using a validated thirteen-item FFQ. Fasting blood samples were collected and measured for total cholesterol (TC), TAG, HDL-cholesterol and LDL-cholesterol. Multivariate linear and logistic regression models were employed to examine the relationship between spicy food and serum lipids levels according to the spicy food flavour and intake frequency, respectively.

Setting: A cross-sectional study in Henan Province.

Participants: 38 238 participants aged 18-79 years old.

Results: Spicy flavour and intake frequency were consistently associated with decreased TC and non-HDL-cholesterol levels but mildly associated with elevated TAG levels. Each level increment in spicy flavour was inversely associated with high TC (OR: 0·91; 95 % CI 0·88, 0·93) and high non-HDL-cholesterol (OR: 0·88; 95 % CI 0·85, 0·91) but positively associated with high TAG (OR: 1·04; 95 % CI 1·01, 1·07). Similarly, 1-d increment in spicy food intake frequency was also inversely associated with high TC (OR: 0·92; 95 % CI 0·91, 0·94) and high non-HDL-cholesterol (OR: 0·91; 95 % CI 0·89, 0·93) but positively associated with high TAG (OR: 1·04; 95 % CI 1·02, 1·06).

Conclusions: Spicy food intake was mildly associated with increased risk of abnormal TAG level, significantly associated with decreased risk of abnormal TC and non-HDL levels. Spicy food intake may be contribute to the management of lipid levels.
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http://dx.doi.org/10.1017/S1368980021001853DOI Listing
April 2021

Study on Reference Range of Zinc, Copper and Copper/Zinc Ratio in Childbearing Women of China.

Nutrients 2021 Mar 15;13(3). Epub 2021 Mar 15.

National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Key Laboratory of Trace Element Nutrition, National Health Commission of the People's Republic of China, Beijing 100050, China.

Copper and zinc are both essential elements in humans, that play various biological roles in body functions. Population-based reference values have not yet been established in China especially in childbearing women. The aim of this study is to establish a reference value of Zn, Cu and Cu/Zn ratios in childbearing women aged 18-44 from a representative population in China. A total of 191 healthy childbearing women aged 18-44 years old were enrolled from the China Adult Chronic Disease and Nutrition Surveillance (2015) in this study with a series strict inclusion criteria. Basic biological indicators (weight, height, waist, blood pressure, high-density lipoprotein cholesterol, low-density lipoprotein, total cholesterol, triglyceride, fast glycose, HbA1c, blood pressure, uric acid) and elements levels in plasma and whole blood were collected. The 2.5th to 97.5th was used to represent the reference range of Cu, Zn and Cu/Zn ratio. The reference range of Zn, Cu and Cu/Zn ratio in plasma were 70.46-177.53 µg/dL, 74.30-170.68 µg/dL and 0.54-1.68, respectively. The reference range of Zn, Cu and Cu/Zn ratios in whole blood were 402.49-738.05, 74.63-124.52 and 0.13-0.25 µg/dL, respectively. The reference range of Zn, Cu and Cu/Zn ratios in plasma and whole blood of healthy Chinese childbearing women could be used as an indicator to evaluate the status of element deficiency and overload.
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http://dx.doi.org/10.3390/nu13030946DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7999022PMC
March 2021

Response to Comment on "Suspect and Nontarget Screening of Per- and Polyfluoroalkyl Substances in Wastewater from a Fluorochemical Manufacturing Park".

Environ Sci Technol 2021 04 30;55(8):5593-5596. Epub 2021 Mar 30.

State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, People's Republic of China.

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http://dx.doi.org/10.1021/acs.est.1c01254DOI Listing
April 2021

Differential functional connectivity of insular subdivisions in de novo Parkinson's disease with mild cognitive impairment.

Brain Imaging Behav 2021 Mar 26. Epub 2021 Mar 26.

Institute of Brain Functional Imaging, Nanjing Medical University, 210029, Nanjing, Jiangsu, China.

The insula, consisting of functionally diverse subdivisions, plays a significant role in Parkinson's disease (PD)-related cognitive disorders. However, the functional connectivity (FC) patterns of insular subdivisions in PD remain unclear. Our aim is to investigate the changes in FC patterns of insular subdivisions and their relationships with cognitive domains. Three groups of participants were recruited in this study, including PD patients with mild cognitive impairment (PD-MCI, n = 25), PD patients with normal cognition (PD-NC, n = 13), and healthy controls (HCs, n = 17). Resting-state functional magnetic resonance imaging (rs-fMRI) was used to investigate the FC in insular subdivisions of the three groups. Moreover, all participants underwent a neuropsychological battery to assess cognition so that the relationship between altered FC and cognitive performance could be elucidated. Compared with the PD-NC group, the PD-MCI group exhibited increased FC between the left dorsal anterior insular (dAI) and the right superior parietal gyrus (SPG), and altered FC was negatively correlated with memory and executive function. Compared with the HC group, the PD-MCI group showed significantly increased FC between the right dAI and the right median cingulate and paracingulate gyri (DCG), and altered FC was positively related to attention/working memory, visuospatial function, and language. Our findings highlighted the different abnormal FC patterns of insular subdivisions in PD patients with different cognitive abilities. Furthermore, dysfunction of the dAI may partly contribute to the decline in executive function and memory in early drug-naïve PD patients.
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http://dx.doi.org/10.1007/s11682-021-00471-2DOI Listing
March 2021

Consistency and Stability of Motor Subtype Classifications in Patients With Parkinson's Disease.

Front Neurosci 2021 1;15:637896. Epub 2021 Mar 1.

Department of Neurology, The Affiliated Brain Hospital of Nanjing Medical University, Nanjing, China.

Objective: Patients with Parkinson's disease (PD) are commonly classified into subtypes based on motor symptoms. The aims of the present study were to determine the consistency between PD motor subtypes, to assess the stability of PD motor subtypes over time, and to explore the variables influencing PD motor subtype stability.

Methods: This study was part of a longitudinal study of PD patients at a single center. Based on three different motor subtype classification systems proposed by Jankovic, Schiess, and Kang, patients were respectively categorized as tremor-dominant/indeterminate/postural instability and gait difficulty (TD/indeterminate/PIGD), TD /mixed /akinetic-rigid (ARS), or TD /mixed /AR at baseline evaluation and then re-assessed 1 month later. Demographic and clinical characteristics were recorded at each evaluation. The consistency between subtypes at baseline evaluation was assessed using Cohen's kappa coefficient (κ). Additional variables were compared between PD subtype groups using the two-sample -test, Mann-Whitney -test or Chi-squared test.

Results: Of 283 newly diagnosed, untreated PD patients, 79 were followed up at 1 month. There was fair agreement between the Jankovic, Schiess, and Kang classification systems (κ = 0.383 ± 0.044, κ = 0.360 ± 0.042, κ = 0.368 ± 0.038). Among the three classification systems, the Schiess classification was the most stable and the Jankovic classification was the most unstable. The non-motor symptoms questionnaire (NMSQuest) scores differed significantly between PD patients with stable and unstable subtypes based on the Jankovic classification ( = 0.008), and patients with a consistent subtype had more severe NMSQuest scores than patients with an inconsistent subtype.

Conclusion: Fair consistency was observed between the Jankovic, Schiess, and Kang classification systems. For the first time, non-motor symptoms (NMSs) scores were found to influence the stability of the TD/indeterminate/PIGD classification. Our findings support combining NMSs with motor symptoms to increase the effectiveness of PD subtypes.
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http://dx.doi.org/10.3389/fnins.2021.637896DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7957002PMC
March 2021

Interpenetrating interfaces for efficient perovskite solar cells with high operational stability and mechanical robustness.

Nat Commun 2021 Feb 12;12(1):973. Epub 2021 Feb 12.

School of Engineering, Brown University, Providence, 02912, RI, USA.

The perovskite solar cell has emerged rapidly in the field of photovoltaics as it combines the merits of low cost, high efficiency, and excellent mechanical flexibility for versatile applications. However, there are significant concerns regarding its operational stability and mechanical robustness. Most of the previously reported approaches to address these concerns entail separate engineering of perovskite and charge-transporting layers. Herein we present a holistic design of perovskite and charge-transporting layers by synthesizing an interpenetrating perovskite/electron-transporting-layer interface. This interface is reaction-formed between a tin dioxide layer containing excess organic halide and a perovskite layer containing excess lead halide. Perovskite solar cells with such interfaces deliver efficiencies up to 22.2% and 20.1% for rigid and flexible versions, respectively. Long-term (1000 h) operational stability is demonstrated and the flexible devices show high endurance against mechanical-bending (2500 cycles) fatigue. Mechanistic insights into the relationship between the interpenetrating interface structure and performance enhancement are provided based on comprehensive, advanced, microscopic characterizations. This study highlights interface integrity as an important factor for designing efficient, operationally-stable, and mechanically-robust solar cells.
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http://dx.doi.org/10.1038/s41467-021-21292-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7881119PMC
February 2021

Association of plant-based diet and type 2 diabetes mellitus in Chinese rural adults: The Henan Rural Cohort Study.

J Diabetes Investig 2021 Feb 9. Epub 2021 Feb 9.

Department of Epidemiology and Health Statistics, College of Public Health, Zhengzhou University, Zhengzhou, China.

Aims/introduction: Studies have found that a plant-based diet was associated with a lower risk of type 2 diabetes, but evidence is scarce on such associations in China. The aim of this study was to investigate whether a plant-based diet is related to a lower risk of type 2 diabetes among Chinese adults.

Materials And Methods: A total of 37,985 participants were enrolled from the Henan Rural Cohort Study. An overall plant-based diet index (PDI) was created by assigning positive and reverse scores to 12 commonly consumed food groups. Multivariate logistic regression models and restricted cubic spline analysis were performed to estimate the odds ratio (OR) and 95% confidence interval (95% CI).

Results: After multivariable adjustment, the risk of type 2 diabetes was inversely associated with the PDI (extreme-quartile OR = 0.88, 95% CI: 0.79-0.98; P = 0.027), the risk associated with a 1 standard deviation (SD) increase in PDI was 4% lower (95% CI, 0.93-1.00; P  = 0.043) for type 2 diabetes. Moreover, the odds of type 2 diabetes was decreased with an increment of PDI after fitting restricted cubic splines (P  < 0.01).

Conclusions: Among Chinese populations, diets higher in plant foods and lower in animal foods were associated with a reduced risk of type 2 diabetes.
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http://dx.doi.org/10.1111/jdi.13522DOI Listing
February 2021

Gender-Specific Inverse Associations Between Beans Intake, Serum Urate Levels, and Hyperuricemia: A Cross-Sectional Analysis Based on the Henan Rural Cohort Study.

Front Nutr 2020 21;7:593599. Epub 2021 Jan 21.

Department of Epidemiology and Biostatistics, College of Public Health, Zhengzhou University, Zhengzhou, China.

Beans are rich in purines, which are important substances that lead to elevated serum urate, especially exogenous purines. Few studies were conducted to assess the relationship between beans intake and serum urate or hyperuricemia, especially in rural people. The purpose of this study was to validate the association by gender in the rural Chinese population. A total of 38,855 participants aged 18-79 years old were enrolled from the Henan Rural Cohort Study (Registration number: ChiCTR-OOC-15006699). Dietary data were collected using a validated food frequency questionnaire (FFQ). Linear regression models and logistic regression models were used to examine the associations between beans intake and serum urate levels or hyperuricemia. Restricted cubic spline regression was performed to display the dose-response relationship. In multivariate-adjusted linear regression, an inverse correlation was found between beans intake and serum urate level (the highest quartile Q4 vs. the bottom quartile Q1) in both men ( = 0.008) and women ( < 0.001). Per 10-g increment in beans intake was associated with 0.30 μmol/L decreased concentration of serum urate in men and 0.71 μmol/L in women. The multivariate-adjusted odds ratios (ORs) of hyperuricemia were 0.83 (0.71, 0.97) in men and 0.73 (0.63, 0.84) in women (Q4 vs. Q1). Per 10-g increment in beans intake created a 1% decreased risk of hyperuricemia in men and 3% in women. The cubic spline suggested a risk reduction for hyperuricemia with increasing intake of beans. A higher beans intake was associated with a lower serum urate level and a reduced risk of hyperuricemia in both sexes, and the association was more pronounced in women.
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http://dx.doi.org/10.3389/fnut.2020.593599DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7859095PMC
January 2021

Association of Night Sleep Duration and Ideal Cardiovascular Health in Rural China: The Henan Rural Cohort Study.

Front Public Health 2020 11;8:606458. Epub 2021 Jan 11.

Department of Clinical Pharmacology, School of Pharmaceutical Science, Zhengzhou University, Zhengzhou, China.

We aimed to explore the association between night sleep duration and ideal cardiovascular health (ICH) among Chinese rural population. In all, 35,094 participants were included from the Henan Rural Cohort study. Information on sleep was collected using the Pittsburgh Sleep Quality Index. The ICH scores were evaluated. The associations between night sleep duration and ICH were examined using both linear regression and logistic regression models. The mean night sleep duration for all participants was 7.75 ± 1.28 h. Compared with those with night sleep duration of 7 to <9 h by using linear regression model, a significant decrease in ICH scores was observed for participants with shorter [-0.077 (-0.131, -0.024)] and longer [-0.079 (-0.121, -0.036)] night sleep duration. Compared with 7 to <9 h, longer sleep duration [0.919 (0.851, 0.992)] were associated with decreased odds of ideal CVH. Shorter and longer night sleep duration are negatively associated with ICH among rural population. This suggests that it may be beneficial to include night sleep duration assessment in cardiovascular risk screening.
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http://dx.doi.org/10.3389/fpubh.2020.606458DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7830879PMC
May 2021

Health-related quality of life among rural adults with type 2 diabetes mellitus: a cross-sectional study.

Eur J Public Health 2021 Jan 26. Epub 2021 Jan 26.

Department of Epidemiology and Biostatistics, College of Public Health, Zhengzhou University, Zhengzhou, Henan, PR China.

Background: Previous reports about health-related quality of life (HRQoL) of type 2 diabetes mellitus (T2DM) concentrated on general patients rather than patients in rural areas with poor infrastructure and limited resources. Thus, the aims of this study were to evaluate the HRQoL of diabetics in the countryside and explore its influencing factors.

Methods: A total of 23 053 participants aged from 18 to 79 years were drawn from the Henan Rural Cohort Study for this cross-sectional study. The HRQoL of participants were assessed by utility index and VAS-score of European Quality of Life Five Dimension Five Level Scale (EQ-5D-5L) instrument. Binary logistic regression, generalized linear and tobit regression models were used to estimate the potential influencing factors on HRQoL.

Results: This study (23 053 participants) included 2231 T2DM patients with a crude prevalence of 9.68%. The utility index and VAS-score in health group were 0.96 ± 0.10 and 78.85 ± 14.53, while in T2DM group were 0.93 ± 0.15 and 74.09 ± 16.09, respectively. In total, most diabetics reported problem about pain/discomfort dimension. Being old, poverty, low physical activity, and with comorbidities was negatively related to HRQoL of diabetics, while high educational level was positively related to HRQoL.

Conclusion: HRQoL of rural T2DM patients depended on several sociodemographic factors. More attention should be paid to diabetics with poor socioeconomic status in rural areas.

Clinical Trial Registration: The Henan Rural Cohort Study has been registered at Chinese Clinical Trial Register (Registration number: ChiCTR-OOC-15006699) http://www.chictr.org.cn/showproj.aspx?proj=11375.
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http://dx.doi.org/10.1093/eurpub/ckaa247DOI Listing
January 2021

Clinical Value of 3D-Printed Navigation Technology Combined with Neuroendoscopy for Intracerebral Hemorrhage.

Transl Stroke Res 2021 Jan 25. Epub 2021 Jan 25.

Department of Neurosurgery, Dalang Hospital, Dongguan, Guangdong, China.

Intracerebral hemorrhage (ICH) is the most common form of hemorrhagic stroke with high morbidity and mortality. Rapid and massive bleeding may compress the brain tissue, causing space-occupying and pathological effects, such as reduced local cerebral blood flow, acidosis, and inflammatory and immune responses. Although the development of minimally invasive technique provides a new option for the treatment of ICH, their application is limited due to the difficulty in achieving accurate puncture localization under the guidance of the marks on CT. We selected 30 patients treated with neuroendoscopic surgery guided by 3D-printed navigation technology (experimental group) and 30 patients treated with neuroendoscopic surgery guided by hand-painted on the patient's body surface according to the marks on CT (control group). Our results showed that patients in the experimental group had a lower number of intraoperative punctures, shorter operation time, less intraoperative blood loss, higher hematoma clearance rate, and smaller volume of perihematomal edema than the patients in the control group. Moreover, patients in the experimental group had higher Glasgow Coma Scale score at discharge, shorter postoperative hospitalization time and ICU stay, and a lower rate of postoperative complications, despite the lack of statistically significant differences. In addition, no statistically significant differences were observed in mortality and Glasgow Outcome Scale score between the two groups. In conclusion, 3D-printed navigation technology used for the neuroendoscopic hematoma removal is a more reliable and less invasive approach in the treatment of ICH. This technique has great application prospects and deserves promotion in the future clinical practice.
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http://dx.doi.org/10.1007/s12975-021-00893-6DOI Listing
January 2021

Comparison of Three Motor Subtype Classifications in Parkinson's Disease Patients.

Front Neurol 2020 23;11:601225. Epub 2020 Dec 23.

Department of Neurology, Affiliated Brain Hospital of Nanjing Medical University, Nanjing, China.

The aims of this study were to compare the characteristics of three motor subtype classifications in patients with Parkinson's disease (PD) and to find the most suitable motor subtype classification for identifying non-motor symptoms (NMSs). According to previous studies, a total of 256 patients with PD were classified using the tremor-dominant/mixed/akinetic-rigid (TD/mixed/AR), TD/indeterminate/postural instability and gait disturbance (PIGD), and predominantly TD/predominantly PIGD (p-TD/p-PIGD) classification systems. Among the TD/mixed/AR subgroups, the patients with the AR subtype obtained more severe motor scores than the patients with the TD subtype. Among the TD/indeterminate/PIGD subgroups and between the p-TD and p-PIGD subgroups, the patients with the PIGD/p-PIGD subtype obtained more severe scores related to activities of daily living (ADL), motor and non-motor symptoms, including depression, anxiety, and sleep impairment, than the patients with the TD/p-TD subtype. Furthermore, symptoms in the cardiovascular, gastrointestinal, and miscellaneous domains of the Non-motor Questionnaire (NMSQuest) were more prevalent in the patients with the PIGD/p-PIGD subtypes than the patients with the TD/p-TD subtypes. The PIGD/p-PIGD subtypes had more severe ADL, motor and non-motor symptoms than the TD/p-TD subtypes. We disclosed for the first time that the TD/indeterminate/PIGD classification seems to be the most suitable classification among the three motor subtype classifications for identifying NMSs in PD.
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http://dx.doi.org/10.3389/fneur.2020.601225DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7785849PMC
December 2020

Independent and interactive effect of sitting time and physical activity on prevalence of hyperuricemia: the Henan Rural Cohort Study.

Arthritis Res Ther 2021 01 6;23(1). Epub 2021 Jan 6.

Department of Epidemiology and Biostatistics, College of Public Health, Zhengzhou University, 100 Kexue Avenue, Zhengzhou, 450001, Henan, People's Republic of China.

Background: There are few studies on the hyperuricemia (HUA) and moderate to vigorous intensity physical activity (PA) and also hardly regarding sitting time (ST). The purpose of this study was to examine the independent and interactive association of PA and ST with HUA.

Methods: A cross-sectional analysis was performed on 38,855 participants (aged 18-79) enrolled from the Henan Rural Cohort Study at baseline (2015 to 2017). PA and ST levels were assessed by using the International Physical Activity Questionnaire (IPAQ). HUA was defined as a serum uric acid level of > 7.0 mg/dL for males and > 6.0 mg/dL for females. Multivariable logistic regression and linear regression models were applied to examine the independent association between PA or ST and HUA and serum uric acid level. Interaction plots were used to visualize the interaction effects of PA and ST on HUA.

Results: PA level was inversely related with serum uric acid level (β - 0.15, 95% confidence interval (CI) - 0.22, - 0.07), but ST was positively related with uric acid level (β 2.12, 95% CI 1.90, 2.34). Metabolic equivalent (MET-hour/day) was associated with decreased prevalence of HUA (odds ratio (OR) 0.97, 95% CI 0.96, 0.99), while per hour increased for ST was associated with increased HUA (OR 1.05, 95% CI 1.04, 1.06). The interaction of PA and ST was significant (P < 0.001).

Conclusion: Exposure to higher ST was independently related to increased prevalence of HUA, while vigorous PA with a decreased HUA prevalence. Meanwhile, higher daily ST might attenuate the protective effect of PA on HUA.

Trial Registration: The Henan Rural Cohort Study has been registered at Chinese Clinical Trial Register (Registration number: ChiCTR-OOC-15006699 ).
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http://dx.doi.org/10.1186/s13075-020-02385-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7789632PMC
January 2021

Consumption of Dairy Products in Relation to Type 2 Diabetes Mellitus in Chinese People: The Henan Rural Cohort Study and an Updated Meta-Analysis.

Nutrients 2020 Dec 15;12(12). Epub 2020 Dec 15.

Department of Epidemiology and Health Statistics, College of Public Health, Zhengzhou University, Zhengzhou 450001, China.

Recent studies on whether dairy consumption is associated with type 2 diabetes mellitus (T2DM) have yielded inconsistent results, so we explored the relationship between dairy consumption and T2DM through a large-sample, cross-sectional study and a meta-analysis. In the meta-analysis, summary relative risks (RRs) of 23 articles were compiled with a random effects model, and a restricted cubic spline regression model was used to explore whether there is a nonlinear relationship between dairy intake and T2DM risk. This cross-sectional study used baseline data from 38,735 participants of the Henan Rural Cohort study and the association between dairy consumption and T2DM was analyzed by a logistic regression model. The meta-analysis revealed a borderline negative significant association between total dairy intake and risk of T2DM, the RR and 95% confidence interval (CI) was 0.94; (0.89, 1.00), and the risk was lowest at 270 g daily dairy intake. In the cross-sectional study, there were 3654 T2DM patients and 68.3 percent of the respondents had no dairy intake. The average intake of dairy in the total population was 12 g per day. Fully adjusted analyses suggested positive associations, with an odds ratio (OR) comparing the highest with the zero intake of 1.34 (95% CI: 1.22, 1.48) for all participants, which was unaffected by sex. Dairy intake in rural areas of Henan province is low, and we found, in the context of overall low dairy intake, that a high intake was positively associated with T2DM, which is inconsistent with the meta-analysis results suggesting that dairy has marginal protective effects against T2DM.
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http://dx.doi.org/10.3390/nu12123827DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7765212PMC
December 2020

The ADCY9 genetic variants are associated with glioma susceptibility and patient prognosis.

Genomics 2021 Mar 14;113(2):706-716. Epub 2020 Dec 14.

Department of Neurosurgery, The Affiliated Children's Hospital of Xi'an Jiaotong University, Xi'an Children's Hospital, Xi'an 710003, Shaanxi, China. Electronic address:

Purpose: Genetic factor is a risk factor in glioma occurrence. This study was designed to detect the effect of ADCY9 polymorphisms on glioma risk and prognosis.

Methods: We performed a case-control study of 1080 participants (584 cases and 496 controls) to assess the relationship of ADCY9 polymorphisms with the risk and prognosis of glioma among the Chinses Han population. Odds ratios (ORs) and 95% confidence intervals (CIs) were computed to evaluate the relationship between ADCY9 variants and glioma risk. The correlation of SNPs with survival was analyzed by the Cox regression model.

Results: Our study showed that rs2230742 and rs2531992 polymorphisms played protective roles in glioma susceptibility (OR 0.65, p = 0.001; OR 0.73, p = 0.038, respectively). While rs2230742 significantly increased the susceptibility of III-V grade glioma patients (OR 1.50, p = 0.036). Haplotype analysis revealed that CAA haplotype was related to a significantly decreased glioma risk (OR 0.65, p = 0.002). Notably, rs2531995 and rs879620 polymorphisms significantly enhanced death risk in high-grade glioma patients (hazard ratio [HR] 1.36, p = 0.041; HR 1.37, p = 0.042; respectively). For rs2230742 and rs2531992 SNPs, glioma patients had a worse prognosis (HR 2.30, p = 0.021; HR 2.30, p = 0.021; respectively). We further observed that age, chemotherapy, and surgical scope can affect the glioma prognosis.

Conclusion: We firstly studied the association of ADCY9 variants with glioma risk and prognosis, which might give scientific evidence for exploring the molecular mechanism of glioma.
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http://dx.doi.org/10.1016/j.ygeno.2020.12.019DOI Listing
March 2021

Calcium leaching characteristics in landfill leachate collection systems from bottom ash of municipal solid waste incineration.

J Environ Manage 2021 Feb 28;280:111729. Epub 2020 Nov 28.

School of Environment, Tsinghua University, Beijing, 100084, PR China. Electronic address:

Calcium leaching is a critical factor in the clogging of leachate collection systems (LCS), a phenomenon that affects landfill stability and operation. The bottom ash (BA) of municipal solid waste (MSW) incineration plants contains large quantities of calcium-based compounds. Landfilling is the main disposal method for BA in China that intensifies the consequences of LCS clogging. The factors influencing BA calcium leaching were investigated using simulated leachate. The results showed that fine BA particles, low pH values, high temperature, and ratios of leachate to BA solids were conducive to calcium leaching. Calcium leaching was found to be higher in actual leachate than in simulated leachate. At pH = 5, the cumulative calcium dissolution ratios (CDRs) were 83.36% and 31.49% after 20 days of leaching in the actual and simulated leachate, respectively; at pH = 6, the values were 50.67% and 12.06%, respectively. The introduction of landfill gas could decrease the calcium dissolution and leaching rates. When the ratio of leachate to BA solid was 20:1 mL/g, the accumulative CDR values were 45.98% (pH = 6) and 5.80% (pH = 8) without landfill gases, and 4.59% (pH = 6) and 0.48% (pH = 8) with landfill gases. These results provide the scientific basis for clogging risk prediction with respect to calcium leaching in the LCS of landfills. BA landfilling in old landfill areas with relatively high leachate pH and low chemical oxygen demand, as well as when leachate mixed with an appropriate amount of landfill gases, could be feasible measures to reduce calcium leaching and further prevent clogging in LCS.
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http://dx.doi.org/10.1016/j.jenvman.2020.111729DOI Listing
February 2021

Non-Motor Symptoms of the Postural Instability and Gait Difficulty Subtype in De Novo Parkinson's Disease Patients: A Cross-Sectional Study in a Single Center.

Neuropsychiatr Dis Treat 2020 2;16:2605-2612. Epub 2020 Nov 2.

Department of Neurology, Affiliated Brain Hospital of Nanjing Medical University, Nanjing, People's Republic of China.

Background And Purpose: Little is known about non-motor symptoms (NMSs) associated with the postural instability and gait difficulty (PIGD) phenotype, especially in de novo Parkinson's disease (PD) patients. The aims of this study were to compare NMSs between the tremor dominant (TD) and PIGD phenotypes in de novo PD patients and to determine factors that are associated with the PIGD subtype.

Patients And Methods: In a cross-sectional study conducted at our single center, 226 de novo PD patients with a median disease duration of 2 years were recruited. Data, including comprehensive demographics, motor subtypes and NMSs were obtained. Motor subtypes were classified as PIGD and non-PIGD (TD and indeterminate) by Jankovic's method. NMSs were evaluated by the non-motor symptoms questionnaire (NMSQuest).

Results: We identified 73 (32.3%), 34 (15.0%) and 119 (52.7%) patients with TD, intermediate and PIGD subtypes, respectively. Patients with the PIGD subtype had poorer ADL, motor, depression, anxiety, sleep, and non-motor scores compared with those with the TD subtype. In the NMSQuest, the prevalence of cardiovascular, sleep, mood/cognitive and miscellaneous domains was increased in patients with the PIGD subtype compared with patients with the TD subtype. Multivariable forward stepwise logistic regression revealed that the Hamilton Depression Scale (HAMD) [odds ratio (OR), 1.059; 95% confidence interval (CI), 1.016-1.104, = 0.007] and pain (OR, 3.175; 95% CI, 1.695-5.947, < 0.001) exhibit significant discriminative power in differentiating PIGD and non-PIGD groups.

Conclusion: The PIGD group had more severe cardiovascular symptoms, sleep impairments, mood disturbances and pain. We demonstrated for the first time that pain was associated with the PIGD phenotype. Prompt detection and early treatment of NMSs related to the PIGD phenotype may improve patient outcomes.
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http://dx.doi.org/10.2147/NDT.S280960DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7646450PMC
November 2020

Mediation effect of body mass index on the association between spicy food intake and hyperuricemia in rural Chinese adults: the Henan rural cohort study.

BMC Public Health 2020 Oct 29;20(1):1629. Epub 2020 Oct 29.

Department of Epidemiology and Biostatistics, College of Public Health, Zhengzhou University, 100 Kexue Avenue, Zhengzhou, 450001, Henan, PR China.

Background: The relationship of spicy food intake with hyperuricemia remains unknown. The objective of this study was to examine the association between spicy food intake and hyperuricemia, and whether this association was mediated by body mass index (BMI) in Chinese rural population.

Methods: 38, 027 adults aged 18-79 years were recruited from the Henan Rural Cohort Study. Information on spicy food intake was obtained using a validated questionnaire survey. Multivariable logistic regression model was used to estimate the association between spicy food intake and hyperuricemia, multiple linear regression model was performed to estimate the relationships between spicy food intake, BMI and serum urate level. BMI was used as a mediator to evaluate the mediation effect.

Results: After adjusting for potential confounders, compared with no spicy food flavor, the odds ratio (OR) and 95% confidence interval (CI) of mild, middle, and heavy flavor for hyperuricemia were 1.09 (1.00-1.19), 1.10 (0.97-1.24), and 1.21 (1.10-1.46), respectively (P = 0.017). Similarly, compared with those without intake in spicy food, the multivariable adjusted OR (95% CI) of 1-2 days/week, 3-5 days/week, and 6-7 days/week were 1.15 (1.01-1.31), 1.14 (1.01-1.30) and 1.15 (1.05-1.26), respectively (P = 0.007). However, when we further controlling for BMI, the associations were substantially attenuated. Furthermore, mediation analysis showed that BMI play a full mediating role in the relationship of spicy food intake with hyperuricemia.

Conclusion: Spicy food flavor and intake frequency are positively related with hyperuricemia in Chinese rural population. BMI may play a full mediating role in the relationship.

Trial Registration: The Henan Rural Cohort Study registered at Chinese Clinical Trial Register (Registration number: ChiCTR-OOC-15006699 ). Date of registration: 2015-07-06.
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http://dx.doi.org/10.1186/s12889-020-09736-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7596996PMC
October 2020

Long Non-coding RNA MALAT1 Upregulates ZEB2 Expression to Promote Malignant Progression of Glioma by Attenuating miR-124.

Mol Neurobiol 2021 Mar 19;58(3):1006-1016. Epub 2020 Oct 19.

Department of Neurosurgery, Tangdu Hospital, Air Force Medical University, Xi'an, Shaanxi, China.

Long non-coding RNA (lncRNA) metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) has been shown to play a critical role in the development of several malignancies. However, the potential molecular mechanism of MALAT1 in glioma remains unclear. In the present study, we found that the expression of MALAT1 was aberrantly increased in both human glioma tissues and cells and associated with poor prognosis in glioma patients. We further found that MALAT1 silencing significantly inhibited glioma cell proliferation while induced cell cycle arrest and apoptosis. In parallel, knockdown of MALAT1 decreased tumor volume in vivo. These results suggested that MALAT1 acts as a functional oncogene, resulting in the oncogenicity in glioma. Nevertheless, the tumor-suppressive effect of MALAT1 silencing was reversed by miR-124. Besides, the relevance of ZEB2 in tumor progression has been studied in several forms of human cancer, and ZEB2 was identified as a target of miR-124 and negatively regulated by miR-124. MALAT1 overexpression or miR-124 inhibitor led to increased expression of ZEB2. In summary, our study depicts a novel pathway of MALAT1/miR-124/ZEB2 that regulates the progression of glioma and might provide a promising strategy for glioma therapy.
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http://dx.doi.org/10.1007/s12035-020-02165-0DOI Listing
March 2021

Benzo[a]pyrene might be transported by a TonB-dependent transporter in Novosphingobium pentaromativorans US6-1.

J Hazard Mater 2021 02 18;404(Pt A):124037. Epub 2020 Sep 18.

Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen 361102, China; State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361102, China. Electronic address:

Sphingomonads are well known for their ability to efficiently degrade polycyclic aromatic hydrocarbons (PAHs), but little is known about the mechanism of PAH uptake and transport across the cell membrane. RNA sequencing analysis of a sphingomonad, Novosphingobium pentaromativorans US6-1 showed that 38 TonB-dependent transporter (TBDT) genes were significantly upregulated under 5-ring PAH-benzo[a]pyrene (BaP) stress. In order to reveal whether TBDTs are involved in uptake and transport BaP in US6-1, the key TBDT genes were deleted to generate mutants. The results showed that the growth status of these mutants was not different from that of the wild-type strains, but the PAH degradation ability decreased, especially for the mutant strain Δtbdt-11, which did not encode the tbdt-11 gene. Meanwhile, the cell surface hydrophobicity (CSH) of Δtbdt-11 was found to be significantly lower than that of the wild-type strain under BaP stress. Furthermore, the transcriptional activity of genes encoding PAH degradative enzymes was found to be greatly reduced in Δtbdt-11. Confocal microscopy observations showed that US6-1 could transport BaP across the outer membrane, but this transport capacity was significantly reduced in Δtbdt-11 and wild-type US6-1 treated with PMF uncoupler, further confirming that the tbdt-11 gene was associated with PAH active transport.
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http://dx.doi.org/10.1016/j.jhazmat.2020.124037DOI Listing
February 2021

Evaluation of the Associations of GC and CYP2R1 Genes and Gene-Obesity Interactions with Type 2 Diabetes Risk in a Chinese Rural Population.

Ann Nutr Metab 2020 24;76(3):175-182. Epub 2020 Sep 24.

Department of Epidemiology and Biostatistics, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China,

Introduction: Group-specific component (GC) and cytochrome P450 family 2 subfamily R member 1 (CYP2R1) gene polymorphisms and obesity have been associated with an increased risk for development of type 2 diabetes mellitus (T2DM) in Asian populations.

Objective: This study assessed the associations of interactions between GC gene variants and CYP2R1 gene variants and between genes and obesity with T2DM risk.

Methods: A study that included 2,271 subjects was performed. Eight single nucleotide polymorphisms in the GC and CYP2R1 genes were genotyped. Interaction analysis was performed using rs7041 in the GC gene and rs1993116 in the CYP2R1 gene. The effects of multiplicative and additive gene-gene and gene-environment interactions on T2DM risk were assessed.

Results: The T2DM risk was significantly associated with being overweight/obese, abdominal obesity, rs7041, and rs1993116. A significant additive interaction between rs1993116 and rs7041 was associated with T2DM. In addition, there was a significant multiplicative interaction between rs7041 and body mass index (BMI) associated with elevated blood glucose levels, and at a higher BMI (>28.47), the G allele carrier showed a stronger effect than the TT genotype.

Conclusions: The interactions between GC rs7041-CYP2R1 rs1993116 and GC rs7041-BMI may explain the mechanisms by which these factors increase the risk of T2DM development.
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http://dx.doi.org/10.1159/000508024DOI Listing
September 2020

Reclassification of the Taxonomic Framework of Orders , and in Class through Phylogenomic Tree Analysis.

mSystems 2020 Sep 15;5(5). Epub 2020 Sep 15.

State Key Laboratory of Marine Environmental Science and Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen, China

Orders and play important roles in various ecosystems as the keystone taxa of microbiomes. However, the two orders present a close evolutionary relationship, which might have caused taxonomic misinterpretation and resulted in an incorrect understanding of their evolutionary history. In this study, first, we used the 16S rRNA gene sequences of 2,049 species of to build a phylogenetic tree, which demonstrated that reports regarding the evolutionary relationship of orders , , and based on a single conserved gene with a poor resolution have been conflicting; in particular, the major families and of order were separated from orders and Subsequently, we constructed the bac120 trees of all representative reference genomes of class based on 120 ubiquitous single-copy proteins from bacteria and a phylogenomic tree based on the 119 core genes of 257 reference genomes obtained from orders , , and to cross validate and infer their intrinsic evolutionary relationships. These results indicated that two novel orders, ord. nov. and ord. nov., and three novel families, fam. nov., fam. nov., and fam. nov., should be proposed. Additionally, orders and were merged into the order except for families and in class , which currently includes 18 families. Our work sheds some light on the evolutionary history of class , which could facilitate the detection and taxonomic analysis of natural communities. The orders , , and , as three major orders of the largest bacterial class, , play important roles in various ecosystems as the keystone taxa of microbiomes, but their evolutionary relationship is currently polyphyletic and chaotic. Here, we constructed a bac120 tree and core-genome tree and calculated the amino acid identity (AAI) value to explore their intrinsic evolutionary history. In this study, we proposed two novel orders and three novel families. This evolution study vastly reconstructed the taxonomic framework of class and could provide a more distinct perspective on global distribution and evolutionary patterns of these environmental microorganisms.
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http://dx.doi.org/10.1128/mSystems.00543-20DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7498684PMC
September 2020

Levodopa Changes Functional Connectivity Patterns in Subregions of the Primary Motor Cortex in Patients With Parkinson's Disease.

Front Neurosci 2020 8;14:647. Epub 2020 Jul 8.

Department of Neurology, The Affiliated Brain Hospital of Nanjing Medical University, Nanjing, China.

Background: The primary motor cortex (M1) is a critical node in Parkinson's disease (PD)-related motor circuitry; however, the functional roles of its subregions are poorly understood. In this study, we investigated changes in the functional connectivity patterns of M1 subregions and their relationships to improved clinical symptoms following levodopa administration.

Methods: Thirty-six PD patients and 37 healthy controls (HCs) were enrolled. A formal levodopa challenge test was conducted in the PD group, and the Unified Parkinson's Disease Rating Scale motor section (UPDRS-III) was assessed before (off state) and 1 h after administration of levodopa (on state). The PD group underwent resting-state functional magnetic resonance imaging in both off and on states, whereas the HC group was scanned once. We used the Human Brainnetome Atlas template to subdivide M1 into twelve regions of interest (ROIs). Functional connectivity (FC) was compared between PD on and off states [paired -test, voxel-level < 0.001, cluster-level < 0.05, Gaussian random field (GRF) correction] and between patients and HC (two-sample -test voxel-level < 0.001, cluster-level < 0.05). Correlations between ΔFC (differences in FC between PD off and on states) and clinical symptom improvements were examined.

Results: There was decreased FC between the right caudal dorsolateral area 6 and the anterior cingulate gyrus (ACC), the right upper limb region and the left medial dorsal thalamus (mdTHA), as well as increased FC between the left tongue and larynx region and the left medial frontal gyrus. ΔFC between the right caudal dorsolateral area 6 and ACC was positively correlated with improvements in UPDRS-III total scores as well as the rigidity (item 22) and bradykinesia (items 23-26 and 31) subscores. ΔFC between the right upper limb region and left thalamus was positively correlated with improvements in the left upper limb tremor (items 20c and 21b) and postural tremor (item 21b) subscores.

Conclusions: Our results reveal novel information regarding the underlying mechanisms in the motor circuits in the M1 and a promising way to explore the internal function of the M1 in PD patients. Notably, M1 is a potential therapeutic target in PD, and the exploration of its subregions provides a basis and a source of new insights for clinical intervention and precise drug treatment.
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http://dx.doi.org/10.3389/fnins.2020.00647DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7360730PMC
July 2020

Age at menopause, body mass index, and risk of type 2 diabetes mellitus in postmenopausal Chinese women: The Henan Rural Cohort study.

Nutr Metab Cardiovasc Dis 2020 07 8;30(8):1347-1354. Epub 2020 Apr 8.

Department of Epidemiology and Health Statistics, College of Public Health, Zhengzhou University, 100 Kexue Avenue, Zhengzhou, Henan, China. Electronic address:

Background And Aim: The present study was conducted to explore the stratified and joint effects of age at menopause and body mass index (BMI) with the risk of type 2 diabetes mellitus (T2DM) in Chinese rural adults.

Methods And Results: A total of 15,406 postmenopausal Chinese women were included in this study. Multivariable logistic regression analysis was used to quantify the stratified and joint effects of age at menopause and BMI on T2DM. Overall, the mean age at menopause and BMI was 48.8 ± 4.7 years and 25.1 ± 3.6 kg/m, respectively. In general, data suggest that: 1) women with BMI ≥ 24 had a higher risk of T2DM, irrespective of age at menopause; 2) in women with BMI < 24, later menopause had a higher risk of T2DM (OR, 1.52; 95% CI, 1.16-2.01); 3) the risk of T2DM was higher only in patients with early or normal age at menopause and BMI ≥ 24, with 0R (95% CI) of (1.58, 1.28-1.94) and (1.48, 1.31-1.67), respectively.

Conclusion: Our findings suggest that: 1) women with BMI ≥ 24 had a higher risk of T2DM, irrespective of age at menopause; 2) in women with BMI < 24, a higher risk of T2DM was found only in those with later menopause; 3) women with later menopause had a higher risk of T2DM, irrespective of BMI; 4) in patients with early or normal age at menopause, a higher risk of T2DM was found only in patients with BMI ≥ 24.

The Chinese Clinical Trial Registration: ChiCTR-OOC-1500669(URL:http://www.chictr.org.cn/showproj.aspx?proj=11375).
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http://dx.doi.org/10.1016/j.numecd.2020.04.003DOI Listing
July 2020

Genome re-sequencing and reannotation of the Escherichia coli ER2566 strain and transcriptome sequencing under overexpression conditions.

BMC Genomics 2020 Jun 16;21(1):407. Epub 2020 Jun 16.

State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen, 361102, Fujian, China.

Background: The Escherichia coli ER2566 strain (NC_CP014268.2) was developed as a BL21 (DE3) derivative strain and had been widely used in recombinant protein expression. However, like many other current RefSeq annotations, the annotation of the ER2566 strain was incomplete, with missing gene names and miscellaneous RNAs, as well as uncorrected annotations of some pseudogenes. Here, we performed a systematic reannotation of the ER2566 genome by combining multiple annotation tools with manual revision to provide a comprehensive understanding of the E. coli ER2566 strain, and used high-throughput sequencing to explore how the strain adapted under external pressure.

Results: The reannotation included noteworthy corrections to all protein-coding genes, led to the exclusion of 190 hypothetical genes or pseudogenes, and resulted in the addition of 237 coding sequences and 230 miscellaneous noncoding RNAs and 2 tRNAs. In addition, we further manually examined all 194 pseudogenes in the Ref-seq annotation and directly identified 123 (63%) as coding genes. We then used whole-genome sequencing and high-throughput RNA sequencing to assess mutational adaptations under consecutive subculture or overexpression burden. Whereas no mutations were detected in response to consecutive subculture, overexpression of the human papillomavirus 16 type capsid led to the identification of a mutation (position 1,094,824 within the 3' non-coding region) positioned 19-bp away from the lacI gene in the transcribed RNA, which was not detected at the genomic level by Sanger sequencing.

Conclusion: The ER2566 strain was used by both the general scientific community and the biotechnology industry. Reannotation of the E. coli ER2566 strain not only improved the RefSeq data but uncovered a key site that might be involved in the transcription and translation of genes encoding the lactose operon repressor. We proposed that our pipeline might offer a universal method for the reannotation of other bacterial genomes with high speed and accuracy. This study might facilitate a better understanding of gene function for the ER2566 strain under external burden and provided more clues to engineer bacteria for biotechnological applications.
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http://dx.doi.org/10.1186/s12864-020-06818-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7296898PMC
June 2020

Management of incidental anterior skull base large and giant meningiomas in elderly patients.

J Neurooncol 2020 Jul 11;148(3):481-488. Epub 2020 Jun 11.

Department of Neurosurgery, Tangdu Hospital, Air Force Medical University, No. 569 Xinsi Road, Xi'an, 710038, Shaanxi, China.

Introduction: The incidence of meningiomas among the elderly is considered to be high, and are at increased risk of severe morbidity and mortality following surgery due to their aging physiology and unexpected comorbidities. This study aimed to evaluate the optimal management strategies of meningiomas in elderly patients.

Methods: We retrospectively analyzed 150 patients with incidental large (≥ 3 cm) and giant (≥ 6 cm) anterior skull base meningiomas from 2009 to 2018. These patients were divided into elderly group (≥ 65 years, n = 70) and younger group (< 65 years, n = 80). Information of patients with regard to their medical records, operative details, relevant imaging, and follow-up data were obtained from their respective electronic medical records.

Results: The elderly patients had significantly longer length of hospital stay (15.9 ± 3.5) compared to younger patients (13.6 ± 3.6, P < 0.001). Karnofsky Performance Scale (KPS) at discharge was significantly lower in elderly group when compared to younger group (P = 0.04). However, the KPS at 1-year after surgery was similar between the two groups. In addition, there was no significant difference in the incidence of surgical complications between the two groups. Multivariate regression analysis of postoperative complications revealed blood loss ≥ 800 mL (P = 0.007) and BMI (< 18.5 or ≥ 24, P < 0.001) as risk factors, rather than age.

Conclusions: Surgical resection in elderly patients with incidental anterior skull base large and giant meningiomas is considered to be a safe and effective therapeutic option owing to acceptable mortality, postoperative complications and postoperative clinical outcomes.
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http://dx.doi.org/10.1007/s11060-020-03484-xDOI Listing
July 2020