Publications by authors named "Yuqi Li"

97 Publications

Risk Stratification and Efficacy of Spironolactone in Patients with Heart Failure with Preserved Ejection Fraction: Secondary Analysis of the TOPCAT Randomized Clinical Trial.

Cardiovasc Drugs Ther 2021 Apr 1. Epub 2021 Apr 1.

Cardiology Department, First Affiliated Hospital of Sun Yat-Sen University, 58 Zhongshan 2nd Road, Guangzhou, 510080, China.

Purpose: We aimed to develop a simple risk score for patients with HFpEF and assessed the efficacy of spironolactone across baseline risk.

Methods: We developed risk stratification scheme for cardiovascular death in placebo arm of the Treatment of Preserved Cardiac Function Heart Failure with an Aldosterone Antagonist trial (TOPCAT). We screened candidate risk indicators and determined strong risk predictors using COX regression. The absolute risk reduction (ARR) in cardiovascular death with spironolactone was evaluated across baseline risk groups. COX regressions were performed to assess the hazard ratios (HRs) of spironolactone therapy for cardiovascular death and drug discontinuation in each risk category.

Results: A simple risk score scheme was constructed based on five risk indicators weighted by estimates from the model, including age, diastolic blood pressure, renal dysfunction, white blood cell, and left ventricular ejection fraction. The risk score scheme showed good discrimination in placebo cohort (C index=0.70). ARR with spironolactone therapy was observed only in patients at very high risk (7.9%). Spironolactone therapy significantly reduced the risk of cardiovascular death in the very high-risk group (HR: 0.57; 95%CI, 0.39-0.84; P =0.005 and P for interaction 0.03) but showed similar risk of drug discontinuation across risk categories (P for interaction=0.928).

Conclusion: This simple risk score stratifies patients with HFpEF by their baseline risk of cardiovascular death. Patients at very high risk derive great benefits from spironolactone therapy. This easy-to-use risk score provides a practical tool that can facilitate risk stratification and tailoring therapy for those who benefit most from spironolactone.

Trial Registration: ClinicalTrials.gov Identifier: NCT00094302.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10557-021-07178-yDOI Listing
April 2021

Improving Corrosion Protection and Friction Resistance of Q235 Steel by Combining Noncovalent Action and Rotating Coating Method.

ACS Omega 2021 Mar 9;6(11):7434-7443. Epub 2021 Mar 9.

Key Laboratory of New Processing Technology for Nonferrous Metals and Materials, Ministry of Education, College of Materials Science and Engineering, Guilin University of Technology, Guilin 541004, China.

Developing waterborne epoxy (WEP) coatings with excellent corrosion resistance and tribological properties is a key aspect to solve the damage of Q235 steel. In this work, perylene bisimide (PBI) derivatives dispersion graphene (GR) were prepared by a π-π stacking, the highly orientated PBI/GR/WEP coating will be prepared by the rotating coating method. Especially, the impedance value reached about 10 Ω·cm when the PBI and GR ratio is 1:1. The impedance value of PBI/GR/WEP coating increased by 3 orders of magnitude compared with that of pure WEP coating (10 Ω·cm). Additionally, the coefficient of friction of the coatings was 0.33; compared with that of WEP, the coefficient of friction decreased by 48%, and the wear resistance increased by 87.6%. The results show that the PBI/GR/WEP coatings exhibited excellent corrosion resistance and wear resistance properties due to the good dispersion and high orientation of PBI/GR in WEP. It is anticipated that our current work would guide the ongoing efforts to develop a more efficient method to overcome the poor dispersion of GR in waterborne epoxy resin and provide a green coating with excellent corrosion resistance and wear resistance properties.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsomega.0c05866DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7992059PMC
March 2021

AUXIN RESPONSE FACTOR 18-HISTONE DEACETYLASE 6 module regulates floral organ identity in rose (Rosa hybrida).

Plant Physiol 2021 Mar 17. Epub 2021 Mar 17.

State Key Laboratory of Agrobiotechnology, Beijing Key Laboratory of Development and Quality Control of Ornamental Crops, Department of Ornamental Horticulture, College of Horticulture, China Agricultural University, Beijing 100193, China.

The phytohormone auxin plays a pivotal role in floral meristem initiation and gynoecium development, but whether and how auxin controls floral organ identity remain largely unknown. Here, we found that auxin levels influence organ specification, and changes in auxin levels influence homeotic transformation between petals and stamens in rose (Rosa hybrida). The PIN-FORMED-LIKES (PILS) gene RhPILS1 governs auxin levels in floral buds during floral organogenesis. RhAUXIN RESPONSE FACTOR 18 (RhARF18), whose expression decreases with increasing auxin content, encodes a transcriptional repressor of the C-class gene RhAGAMOUS (RhAG) and controls stamen-petal organ specification in an auxin-dependent manner. Moreover, RhARF18 physically interacts with the histone deacetylase (HDA) RhHDA6. Silencing of RhHDA6 increases H3K9/K14 acetylation levels at the site adjacent to the RhARF18-binding site in the RhAG promoter and reduces petal number, indicating that RhARF18 might recruit RhHDA6 to the RhAG promoter to reinforce the repression of RhAG transcription. We propose a model for how auxin homeostasis controls floral organ identity via regulating transcription of RhAG.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/plphys/kiab130DOI Listing
March 2021

Association of Mid- to Late-Life Blood Pressure Patterns With Risk of Subsequent Coronary Heart Disease and Death.

Front Cardiovasc Med 2021 15;8:632514. Epub 2021 Feb 15.

Department of Cardiology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.

The elevated blood pressure (BP) at midlife or late-life is associated with cardiovascular disease and death. However, there is limited research on the association between the BP patterns from middle to old age and incident coronary heart disease (CHD) and death. A cohort of the Atherosclerosis Risk in Communities (ARIC) Study enrolled 9,829 participants who attended five in-person visits from 1987 to 2013. We determined the association of mid- to late-life BP patterns with incident CHD and all-cause mortality using multivariable-adjusted Cox proportional hazards models. During a median of 16.7 years of follow-up, 3,134 deaths and 1,060 CHD events occurred. Compared with participants with midlife normotension, the adjusted hazard ratio for all-cause mortality and CHD was 1.14 (95% CI, 1.04-1.25) and 1.28 (95% CI, 1.10-1.50) in those with midlife hypertension, respectively. In further analyses, compared with a pattern of sustained normotension from mid- to late-life, there was no significant difference for the risk of incident death (HR, 1.15; 95% CI, 0.96-1.37) and CHD (HR, 1.33; 95% CI, 0.99-1.80) in participants with a pattern of midlife normotension and late-life hypertension with effective BP control. A higher risks of death and CHD were found in those with pattern of mid- to late-life hypertension with effective BP control (all-cause mortality: HR, 1.24; 95% CI, 1.08-1.43; CHD: HR, 1.65; 95% CI 1.30-2.09), pattern of midlife normotension and late-life hypertension with poor BP control (all-cause mortality: HR, 1.27; 95% CI, 1.12-1.44; CHD: HR, 1.53; 95% CI, 1.23-1.92), and pattern of mid- to late-life hypertension with poor BP control (all-cause mortality: HR, 1.49; 95% CI, 1.30-1.71; CHD: HR, 1.87; 95% CI, 1.48-2.37). The current findings underscore that the management of elderly hypertensive patients should not merely focus on the current BP status, but the middle-aged BP status. To achieve optimal reductions in the risk of CHD and death, it may be necessary to prevent, diagnose, and manage of hypertension throughout middle age.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fcvm.2021.632514DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7917074PMC
February 2021

Fangchinoline exerts anticancer effects on colorectal cancer by inducing autophagy via regulation AMPK/mTOR/ULK1 pathway.

Biochem Pharmacol 2021 Feb 18;186:114475. Epub 2021 Feb 18.

Institute of Tissue Engineering and Stem Cells, Cancer Biotherapy Key Laboratory of Nanchong Nanchong Central Hospital, The Second Clinical Medical College, North Sichuan Medical College, Nanchong 637000, China. Electronic address:

Autophagy has become a promising target for cancer therapy. Fangchinoline (Fan) has been shown to exert anticancer effects in some types of cancers. However, the anticancer effects on colorectal cancer (CRC) and the underlying mechanisms have never been elucidated. More specifically, regulation of autophagy in CRC by Fan has never been reported before. In the present study, Fan was found to induce apoptosis and autophagic flux in the CRC cell lines HT29 and HCT116, which was reflected by the enhanced levels of LC3-II protein and p62 degradation, and the increased formation of autophagosomes and puncta formation by LC3-II. Meanwhile, combination with the early-stage autophagy inhibitor 3-methyladenine (3-MA) but not the late-stage autophagy inhibitor chloroquine (CQ) further increased Fan-induced cell death, which suggested the cytoprotective function of autophagy induced by Fan in both HT29 and HCT116 cells. Moreover, Fan treatment demonstrated a dose- and time-dependently increase in the phosphorylation of AMPK and decrease in the phosphorylation of mammalian target of rapamycin (mTOR) and ULK1, leading to the activation of the AMPK/mTOR/ULK1 signaling pathway. Furthermore, in the HT29 xenograft model, Fan inhibited tumor growth in vivo. These results indicate that Fan inhibited CRC cell growth both in vitro and in vivo and revealed a new molecular mechanism involved in the anticancer effect of Fan on CRC, suggesting that Fan is a potent autophagy inducer and might be a promising anticancer agent.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bcp.2021.114475DOI Listing
February 2021

A Hydrophobic Sisal Cellulose Microcrystal Film for Fire Alarm Sensors.

Nano Lett 2021 Mar 16;21(5):2104-2110. Epub 2021 Feb 16.

Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Material Science and Engineering, and Collaborative Research Center of Molecular Engineering for Theranostics, Hunan University, Changsha 410082, China.

At present, environmentally friendly biobased flexible films are of particular interest as next-generation fireproof packaging and sensor materials. To reduce the moisture uptake and fire risks induced by hygroscopic and flammable biobased films, we report a simple and green approach to develop a hydrophobic, flame-retardant composite film with synergetic benefit from soy protein isolate (SPI), sisal cellulose microcrystals (MSF--COOH), graphene nanosheets (GN), and citric acid (CA). Compared with SPI/MSF--COOH composite films, the as-prepared SPI/MSF--COOH/CA/GN composite films have significantly improved water resistance and can maintain excellent physical structure and good electrical conductivity in an ethanol flame. This work opens a pathway for the development of novel fire-retardant fire alarm biosensors.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.nanolett.0c04789DOI Listing
March 2021

Genome sequence and evolution of Betula platyphylla.

Hortic Res 2021 Feb 11;8(1):37. Epub 2021 Feb 11.

State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University), Harbin, China.

Betula L. (birch) is a pioneer hardwood tree species with ecological, economic, and evolutionary importance in the Northern Hemisphere. We sequenced the Betula platyphylla genome and assembled the sequences into 14 chromosomes. The Betula genome lacks evidence of recent whole-genome duplication and has the same paleoploidy level as Vitis vinifera and Prunus mume. Phylogenetic analysis of lignin pathway genes coupled with tissue-specific expression patterns provided clues for understanding the formation of higher ratios of syringyl to guaiacyl lignin observed in Betula species. Our transcriptome analysis of leaf tissues under a time-series cold stress experiment revealed the presence of the MEKK1-MKK2-MPK4 cascade and six additional mitogen-activated protein kinases that can be linked to a gene regulatory network involving many transcription factors and cold tolerance genes. Our genomic and transcriptome analyses provide insight into the structures, features, and evolution of the B. platyphylla genome. The chromosome-level genome and gene resources of B. platyphylla obtained in this study will facilitate the identification of important and essential genes governing important traits of trees and genetic improvement of B. platyphylla.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41438-021-00481-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7878895PMC
February 2021

Interactions between Anaerobic Fungi and Methanogens in the Rumen and Their Biotechnological Potential in Biogas Production from Lignocellulosic Materials.

Microorganisms 2021 Jan 17;9(1). Epub 2021 Jan 17.

Laboratory of Gastrointestinal Microbiology, National Center for International Research on Animal Gut Nutrition, Nanjing Agricultural University, Nanjing 210095, China.

Anaerobic fungi in the digestive tract of herbivores are one of the critical types of fiber-degrading microorganisms present in the rumen. They degrade lignocellulosic materials using unique rhizoid structures and a diverse range of fiber-degrading enzymes, producing metabolic products such as H/CO, formate, lactate, acetate, and ethanol. Methanogens in the rumen utilize some of these products (e.g., H and formate) to produce methane. An investigation of the interactions between anaerobic fungi and methanogens is helpful as it provides valuable insight into the microbial interactions within the rumen. During the last few decades, research has demonstrated that anaerobic fungi stimulate the growth of methanogens and maintain methanogenic diversity. Meanwhile, methanogens increase the fiber-degrading capability of anaerobic fungi and stimulate metabolic pathways in the fungal hydrogenosome. The ability of co-cultures of anaerobic fungi and methanogens to degrade fiber and produce methane could potentially be a valuable method for the degradation of lignocellulosic materials and methane production.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/microorganisms9010190DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7830786PMC
January 2021

Analysis of multiple cytokines in aqueous humor of patients with idiopathic macular hole.

BMC Ophthalmol 2021 Jan 11;21(1):27. Epub 2021 Jan 11.

Department of Ophthalmology, The Fourth Hospital of Shenyang, 110016, Shenyang, Liaoning, People's Republic of China.

Background: Idiopathic macular holes are common ophthalmic manifestations with unknown pathogenesis. Thus far, there has been minimal research regarding the causes of idiopathic macular holes, especially with respect to the underlying immune mechanism. To provide clarity regarding the treatment and prognosis of idiopathic macular holes, specifically regarding the levels of cytokines in affected patients, this study examined and analyzed multiple cytokine levels in aqueous humor from patients with idiopathic macular holes.

Methods: This comparative cross-sectional study included 38 patients in two groups: a cataract control group (n = 17) and an idiopathic macular hole group (n = 21). The levels of 48 cytokines in aqueous humor were detected by multiplex analysis with antibody-coupled magnetic beads. The Kolmogorov-Smirnov test was used to check whether the data were normally distributed; Student's t-test and the Mann-Whitney U test were used to assess differences in cytokine levels between the two groups. Spearman correlation analysis was used to assess relationships among cytokine levels in the experimental group. Signaling pathways containing cytokines with significantly different expression in the experimental group were identified.

Results: There were significant differences in aqueous humor cytokine levels between patients with idiopathic macular holes and patients in the cataract control group. Notably, hepatocyte growth factor (p = 0.0001), GM-CSF (p = 0.0111), and IFN-γ (p = 0.0120) were significantly upregulated in the experimental group, while TNF-α (p = 0.0032), GRO-α (p < 0.0001), and MIF (p < 0.0001) were significantly downregulated in the experimental group. Furthermore, the GM-CSF level showed significant positive correlations with levels of IL-1 (r = 0.67904, p < 0.001), IL-4 (r = 0.76017, p < 0.001), and IFN-γ (r = 0.59922, p = 0.004097) in the experimental group. Moreover, the levels of nerve growth factor and hepatocyte growth factor showed a significant positive correlation (r = 0.64951, p = 0.001441) in the experimental group.

Conclusions: Patients with idiopathic macular holes showed significant variation in aqueous humor immune response after the onset of hole formation, including the recruitment of immune cells and regulation of cytokine expression. Our findings also suggest that it is not appropriate to use patients with macular holes as the control group in studies of aqueous humor cytokine levels in ophthalmic diseases.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12886-020-01782-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7802234PMC
January 2021

Exogenous Strigolactones alleviate KCl stress by regulating photosynthesis, ROS migration and ion transport in Malus hupehensis Rehd.

Plant Physiol Biochem 2021 Feb 17;159:113-122. Epub 2020 Dec 17.

College of Horticulture, Qingdao Agricultural University, Qingdao 266109, China; Qingdao Key Laboratory of Genetic Improvement and Breeding in Horticulture Plants, Qingdao 266109, China. Electronic address:

Aims: In recent years, the application of large amounts of potash fertilizer in apple orchards leads to worsening KCl stress. Strigolactone (SL), as a novel phytohormone, reportedly participates in plant tolerance to NaCl and drought stresses. However, the underlying mechanism and the effects of exogenous SL on the KCl stress of apple seedlings remain unclear.

Methods: We sprayed different concentrations of exogenous SL on Malus hupehensis Rehd. under KCl stress and measured the physiological indexes like, photosynthetic parameter, content of ROS, osmolytes and mineral element. In addition, the expressions of KCl-responding genes and SL-signaling genes were also detected and analyzed.

Results: Application of exogenous SL protected the chlorophyll and maintained the photosynthetic rate of apple seedlings under KCl stress. Exogenous SL strengthened the enzyme activities of peroxidase and catalase, thereby eliminating reactive oxygen species production induced by KCl stress, promoting the accumulation of proline, and maintaining osmotic balance. Exogenous SL expelled K outside of the cytoplasm and compartmentalized K into the vacuole, increased the contents of Na, Mg, Fe, and Mn in the cytoplasm to maintain the ion homeostasis under KCl stress.

Conclusions: Exogenous SL can regulate photosynthesis, ROS migration and ion transport in apple seedlings to alleviate KCl stress.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.plaphy.2020.12.015DOI Listing
February 2021

Src-mediated Tyr353 phosphorylation of IP3R1 promotes its stability and causes apoptosis in palmitic acid-treated hepatocytes.

Exp Cell Res 2021 Feb 5;399(2):112438. Epub 2021 Jan 5.

Centre for Lipid Research & Key Laboratory of Molecular Biology for Infectious Diseases (Ministry of Education), Institute for Viral Hepatitis, Department of Infectious Diseases, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, 400016, China. Electronic address:

Palmitic acid (PA)-induced hepatocyte apoptosis is critical for the progression of nonalcoholic fatty liver disease (NAFLD). Inositol 1,4,5-trisphosphate receptor type 1 (IP3R1) is an intracellular Ca-release channel and is involved in PA-induced hepatocyte apoptosis. While the expression of IP3R1 is elevated in patients with NAFLD and in hepatocytes treated with PA, it remains unclear how PA promotes the expression of IP3R1. In present study, our results showed that PA induced mitochondrial dysfunction and apoptosis, which is accompanied with the increase of the IP3R1 expression in hepatic cells. The inhibition of IP3R1 expression using siRNA ameliorated the PA-induced mitochondrial dysfunction. Furthermore, PA enhanced the stability of the IP3R1 protein instead of an increase in its mRNA levels. PA also promoted the phosphorylation of IP3R1 at the Tyr353 site and increased the phosphorylation of src in hepatic cells. Moreover, an inhibitor of src kinase (SU6656) significantly reduced the Tyr353 phosphorylation of IP3R1 and decreased its stability. In addition, SU6656 improved mitochondrial function and reduced apoptosis in hepatocytes. Conclusion: PA promotes the Tyr353 phosphorylation of IP3R1 by activating the src pathway and increasing the protein stability of IP3R1, which consequently results in mitochondrial Ca overload and mitochondrial dysfunction in hepatic cells. Our results also suggested that inhibition of the src/IP3R1 pathway, such as by SU6656, may be a novel potential therapeutic approach for the treatment of NAFLD.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.yexcr.2020.112438DOI Listing
February 2021

Association of early elevated cardiac troponin I concentration and longitudinal change after off-pump coronary artery bypass grafting and adverse events: a prospective cohort study.

J Thorac Dis 2020 Nov;12(11):6542-6551

Beijing Anzhen Hospital, Capital Medical University, Beijing, China.

Background: The elevation of troponin after coronary artery bypass grafting (CABG) is common This study aimed to investigate the association between very early cardiac troponin I (cTnI) concentration and its longitudinal change within 24 hours after CABG and 30-day adverse events.

Methods: This study prospectively enrolled 633 patients who underwent isolated off-pump CABG from January 2019 to May 2019. Serum cTnI levels were measured in all patients at two examinations within 24 hours postoperatively (1 hour and 12-18 hours), and a proportional hazards model was used to determine the association between cTnI levels and their change with adverse events, which were defined as a composite of 30-day mortality, stroke, heart failure, myocardial infarction (MI), and ventricular fibrillation.

Results: cTnI levels of the two examinations and absolute change of cTnI levels were significantly higher in the event group than in the non-event group (P<0.01, both). Earlier and later cTnI concentrations were associated with 30-day complications [adjusted hazard ration (HR) 1.598, 95% confidence interval (CI), 1.158-2.204 and HR 1.499, 95% CI, 1.228-1.831, respectively]. With regard to longitudinal change in cTnI levels, participants with persistently high levels of cTnI and those with progression from a low level to high level concentration experienced a significantly increased risk of adverse events than did participants who had a trend of persistently low cTnI levels (HR 3.105, 95% CI, 1.748-5.517 versus HR 2.944, 95% CI, 1.488-5.824).

Conclusions: Longitudinal change in cTnI levels within 24 hours and early cTnI concentrations, even less than 1 hour after CABG, are associated with adverse events. These data will be useful in identifying patients at an increased risk of complications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.21037/jtd-20-1691DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7711399PMC
November 2020

Achieving stable mainstream deammonification process by a novel combinatorial control strategy.

Bioresour Technol 2020 Dec 16;318:124275. Epub 2020 Oct 16.

State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin 150090, China.

In this study, a novel combinatorial control strategy was developed to guarantee a stable mainstream deammonification process, with three critical steps including (a) upflow airwater washing, (b) short-term increased nitrogen loading rate (NLR), and (c) low oxygen supply. Results showed that two upflow double-blanket filter (UDBF) reactors effectively performed the mainstream deammonification process with the nitrogen removal efficiency (NRE) 84.5 ± 2.2% and 84.6 ± 1.6%, respectively and nitrogen removal rate (NRR) 123.8 ± 2.9 and 125.5 ± 6.2 g N·(m·d), respectively. Statistically, temperature and C/N were considered as two vital factors affecting the nitrogen removal pathways, which co-explained 80.9% and 78.4% of the maximum possible contribution of heterotrophic denitrification in both reactors. The deammonification process accounted for more than 59.8% of TN removal in R2 and 54.8% in R1, which cooperated well with heterotrophic denitrification for efficient performance in treating municipal sewage.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biortech.2020.124275DOI Listing
December 2020

Impact of RAAS Blockers on Contrast-Induced Nephropathy in Patients With Renal Insufficiency: A Meta-Analysis.

J Cardiovasc Pharmacol 2020 12;76(6):692-697

Cardiology Department, First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China; and.

The effect of renin-angiotensin-aldosterone system (RAAS) blockers [angiotensin-converting enzyme inhibitors (ACEIs) and angiotensin receptor blockers] on Contrast-induced nephropathy (CIN) is unclear in patients with renal insufficiency. Thus, we conduct a meta-analysis to evaluate the association between the administration of RAAS blockers and CIN in patients with renal insufficiency. We searched PubMed, EMBASE, and Cochrane Library for relevant studies published before September 2019. The primary outcome was the incidence of CIN, and the secondary outcome was the changes in serum creatinine (SCr) from baseline to postprocedure (ΔSCr). Pooled odds ratio (OR) or weighted mean difference (WMD) with their 95% confidence interval (CIs) for the CIN incidence, ΔSCr were used to calculate original data. A total of 8 studies were included in the meta-analysis. Compared with controls, ACEI/angiotensin receptor blocker increased the risk of CIN (OR = 1.61, 95% CI 1.14-2.28, I = 30%; P = 0.007), whereas this association was not significant in Chinese patients (OR = 1.07, 95% CI 0.65-1.77, I = 19%, P = 0.79). The total weighted mean differences of the ΔSCr were 0.06 mg/dL (95% CI: 0.01-0.11, I = 82%; P = 0.03). Administration of RAAS blockers in patients with renal insufficiency was associated with a significantly higher incidence of CIN, whereas it did not show a significant effect on Chinese patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/FJC.0000000000000910DOI Listing
December 2020

Covalent Immobilization of Polypeptides on Polylactic Acid Films and Their Application to Fresh Beef Preservation.

J Agric Food Chem 2020 Sep 3;68(39):10532-10541. Epub 2020 Sep 3.

Engineering Research Center of Food Thermal-Processing Technology, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, People's Republic of China.

To enhance the advantage of a long-term stability and low-toxicity active packaging system, two biodegradable covalent immobilized antibacterial packaging films were developed and applied to fresh beef preservation in this study. A polylactic acid (PLA) film was prepared by the extrusion-casting method. The surface of the PLA film was modified with plasma treatment to generate carboxylic acid groups, and then antibacterial agent nisin or ε-poly lysine (ε-PL) was covalently attached to the modified film surface. Physical, chemical, and antimicrobial properties of films were then characterized. Scanning electron microscopy and water contact angle images confirmed that nisin or ε-PL was successfully grafted onto the film surface. The values of protein loading on the nisin--PLA film and ε-PL--PLA film were 5.34 ± 0.26 and 3.04 ± 0.25 μg of protein/cm on the surface. Microbial analysis indicated that the grafted films effectively inhibit the growth of bacteria. Finally, the effects of the nisin--PLA film or ε-PL--PLA film on physicochemical changes and microbiological counts of fresh beef during cold storage at 4 °C were investigated. The total viable count of the control sample exceeded 7 logarithms of the number of colony forming units per gram (log CFU/g) after 11 days of cold storage (7.01 ± 0.14 log CFU/g) versus 15 days for the ε-PL--PLA film (7.37 ± 0.06 log CFU/g) and the nisin--PLA film (6.83 ± 0.10 log CFU/g). The results showed that covalent immobilized antibacterial packaging films had positive impacts on the shelf life and quality of fresh beef. Therefore, a covalent immobilized antibacterial packaging system could be a novel preservative method for foods.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.jafc.0c03922DOI Listing
September 2020

The enrichment of anaerobic fungi and methanogens showed higher lignocellulose degrading and methane producing ability than that of bacteria and methanogens.

World J Microbiol Biotechnol 2020 Jul 26;36(9):125. Epub 2020 Jul 26.

Laboratory of Gastrointestinal Microbiology, National Center for International Research on Animal Gut Nutrition, Nanjing Agricultural University, Nanjing, 210095, China.

In this study, rumen content was used to obtain three enrichments of anaerobic fungi and methanogens (F + M enrichment), bacteria and methanogens (B + M enrichment), and whole rumen content (WRC enrichment), to evaluate their respective ability to degrade lignocellulose and produce methane. Among the treatments, F + M enrichment elicited the strongest lignocellulose degradation and methane production ability with both rice straw and wheat straw as substrates. Quantitative real-time PCR analysis and diversity analyses of methanogens in the three enrichment treatments demonstrated that F + M had larger number of 16S rRNA gene copies of methanogens and higher relative abundance of Methanobrevibacter, the predominant methanogen found in all enrichments. Caecomyces was the main anaerobic fungal genus for co-culturing to provide substrates for methanogens in this enrichment. Importantly, the F + M enrichment was stable and could be maintained with transfers supplied every 3 days, confirming its potential utility in anaerobic digestion for lignocellulose degradation and methane production.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11274-020-02894-3DOI Listing
July 2020

Multiplexable high-temperature stable and low-loss intrinsic Fabry-Perot in-fiber sensors through nanograting engineering.

Opt Express 2020 Jul;28(14):20225-20235

This paper presents a method of using femtosecond laser inscribed nanograting as low-loss- and high-temperature-stable in-fiber reflectors. By introducing a pair of nanograting inside the core of a single-mode optical fiber, an intrinsic Fabry-Perot interferometer can be created for high-temperature sensing applications. The morphology of the nanograting inscribed in fiber cores was engineered by tuning the fabrication conditions to achieve a high fringe visibility of 0.49 and low insertion loss of 0.002 dB per sensor. Using a white light interferometry demodulation algorithm, we demonstrate the temperature sensitivity, cross-talk, and spatial multiplexability of sensor arrays. Both the sensor performance and stability were studied from room temperature to 1000°C with cyclic heating and cooling. Our results demonstrate a femtosecond direct laser writing technique capable of producing highly multiplexable in-fiber intrinsic Fabry-Perot interferometer sensor devices with high fringe contrast, high sensitivity, and low-loss for application in harsh environmental conditions.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1364/OE.395382DOI Listing
July 2020

Performance of healthy persons under pain in different cognitive load tasks: An event-related potential study on experimental pain individuals.

Brain Behav 2020 08 18;10(8):e01713. Epub 2020 Jun 18.

Department of Rehabilitation Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, China.

Objective: This study aims to determine how brain activities underlying task with different cognitive load would be modulated by the painful state using electroencephalography.

Methods: The pain state was established by spraying capsaicin on subjects' left inner forearm. A total of 20 experimental pain subjects and 20 matched nonpain controls underwent cognitive tasks with electroencephalogram recording. We collected and analyzed behavioral and event-related potential (ERP) data.

Results: High cognitive tasks exhibited significantly longer response times and lower accuracies than low-load tasks. The experimental pain group displayed a significantly lower accuracy than the control group. In addition, the experimental pain group showed no significance between high and low cognitive tasks in early ERP components (amplitude of N1, P2, N2, and early part of late positive potential), whereas the control group exhibited significance between different load tasks. Furthermore, we observed a delay peak energy for delta and theta oscillation in Fz 500-800 ms after the onset for pain persons and high cognitive load tasks.

Conclusions: Inadequate early attention modulation, along with delayed peak energy for brain oscillation (delta and theta), could be accountable for a worse performance in cognitive tasks in the experimental pain group. Thus, cognitive load is a highly considerable factor. Overall, this study offers more insights into how healthy population works with cognitive tasks under pain neurologically.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/brb3.1713DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7428486PMC
August 2020

Effect of dietary gossypol supplement on fermentation characteristics and bacterial diversity in the rumen of sheep.

PLoS One 2020 10;15(6):e0234378. Epub 2020 Jun 10.

Xinjiang Key Laboratory of Meat and Milk Production Herbivore Nutrition, College of Animal Science, Xinjiang Agricultural University, Urumqi, Xinjiang, The People's Republic of China.

The tolerance of ruminants to gossypol, a natural phenolic compound derived from the cotton plant, is greater than that of monogastric animals, partially because of the gossypol-degrading bacteria in the rumen of the ruminants. In this study, we aimed to examine the effect of gossypol supplementation on fermentation characteristics, bacterial α-diversity and community structure in the rumen fluid of sheep to analyse the change of bacterial in response to gossypol. 8 sheep with permanent fistula were randomly divided into 2 groups, a control and gossypol acetate supplementation groups. Sheep in the latter group were supplemented with gossypol acetate at the levels of 600 mg and 1,200 mg/animal per day during the first (S1, days 1 to 27) and subsequent (S2, days 28 to 47) stages. Gossypol supplementation significantly increased the molar proportion of acetate, and decreased the molar proportion of isobutyric acid, butyric acid, and isovaleric acid in the rumen fluid. Gossypol supplementation have no significant effect on bacterial diversity in the rumen fluid. At the phylum level, gossypol had no effect on bacterial community. At the genus level, gossypol supplementation significantly increased the relative abundance of Treponema_2. However, there were no significant differences in the relative abundance of dominant bacterial genera. In conclusion, gossypol supplementation had an effect on molar proportion of acetate, isobutyric acid, butyric acid, and isovaleric acid, but had no significant effect on the bacterial diversity and relative abundance of dominant bacteria in rumen fluid of sheep.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0234378PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7286523PMC
August 2020

Methane Production From Different Parts of Corn Stover via a Simple Co-culture of an Anaerobic Fungus and Methanogen.

Front Bioeng Biotechnol 2020 30;8:314. Epub 2020 Apr 30.

Laboratory of Gastrointestinal Microbiology, National Center for International Research on Animal Gut Nutrition, Nanjing Agricultural University, Nanjing, China.

To determine ways to improve the utilization of corn stover, this study investigated methane production from different parts of corn stover using a simple co-culture of an anaerobic fungus ( species) and methanogen ( species). The simple co-culture was incubated with the stem pith, leaf blade, or stem bark of corn stover (as substrates) at 39°C for 72 h. The results showed that the stem bark had the lowest ( < 0.05) digestibility (38.0 ± 1.36%) and neutral detergent solubles, that is, cell solubles (31.6 ± 0.45%), and the highest ( < 0.05) lignin content (4.8 ± 0.56%). The leaf blade had a significantly higher methane conversion rate (56.6 ± 0.76 mL/g digested substrate) than the stem pith (49.2 ± 1.60 mL/g digested substrate), even though they showed similar levels of methane production (42.4 ± 1.0 mL and 40.9 ± 1.35 mL, respectively). Both the leaf blade and stem pith of corn stover have the potential to produce methane in a simple co-culture of an anaerobic fungus and methanogen.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fbioe.2020.00314DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7204275PMC
April 2020

The in vitro bioavailability of anti-platelet peptides in collagen hydrolysate from silver carp (Hypophthalmichthys molitrix) skin.

Authors:
Yuqi Li Bo Wang Bo Li

J Food Biochem 2020 06 8;44(6):e13226. Epub 2020 Apr 8.

College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China.

Previous animal experiments indicated collagen hydrolysates (CHs) intake decreased platelet release indicators in plasma and highlight potential applications as healthcare supplements to combat cardiovascular disease. The oligopeptides (GPR, GPRG, and GPRGP) have anti-platelet activities. However, it is still unclear whether they are bioactive compounds in CHs from silver carp skin. We investigated the bioavailability of oligopeptides using simulated gastrointestinal digestion and Caco-2 model. Anti-thrombotic activities, in vitro platelet aggregation and formation of platelet thrombus, were evaluated. They resisted gastrointestinal digestion and could be absorbed by Caco-2. Oligopeptides inhibited platelet aggregation induced by adenosine diphosphate and thrombin with IC of 0.160, 0.283, 0.251 mg/ml and 0.714, 1.008, 0.917 mg/ml for GPR, GPRG, and GPRGP, respectively. Oligopeptides prolonged the time of platelet thrombus and inhibited coagulation cascades, but CHs performed no bleeding side effect. These results confirmed that oligopeptides could be used as bioactive compounds of dietary supplements for pre-thrombotic to prevent thrombosis. PRACTICAL APPLICATIONS: Oligopeptides, GPR, GPRG, and GPRGP, derived from silver carp (Hypophthalmichthys molitrix) skin collagen, performed anti-thrombotic activities from their anti-platelet aggregation and anticoagulation activities. But the collagen hydrolysates containing these peptides had no side effect of bleeding in the mice model. Furthermore, this study investigated the bioavailability of these three bioactive peptides by the Caco-2 cells model. Thus, oligopeptides GPR, GPRG, and GPRGP are a potential index of bioactive compounds in the preparation of anti-thrombotic functional foods or healthcare supplements for people at the pre-thrombotic state.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/jfbc.13226DOI Listing
June 2020

Co-cultured methanogen improved the metabolism in the hydrogenosome of anaerobic fungus as revealed by gas chromatography-mass spectrometry analysis.

Asian-Australas J Anim Sci 2020 Dec 13;33(12):1948-1956. Epub 2020 Jan 13.

Laboratory of Gastrointestinal Microbiology, National Center for International Research on Animal Gut Nutrition, Nanjing Agricultural University, Nanjing 210095, China.

Objective: The purpose of this study was to reveal the metabolic shift in the fungus cocultured with the methanogen (Methanobrevibacter thaueri).

Methods: Gas chromatography-mass spectrometry was used to investigate the metabolites in anaerobic fungal (Pecoramyces sp. F1) cells and the supernatant.

Results: A total of 104 and 102 metabolites were detected in the fungal cells and the supernatant, respectively. The partial least squares-discriminant analysis showed that the metabolite profiles in both the fungal cell and the supernatant were distinctly shifted when co-cultured with methanogen. Statistically, 16 and 30 metabolites were significantly (p<0.05) affected in the fungal cell and the supernatant, respectively by the co-cultured methanogen. Metabolic pathway analysis showed that co-culturing with methanogen reduced the production of lactate from pyruvate in the cytosol and increased metabolism in the hydrogenosomes of the anaerobic fungus. Citrate was accumulated in the cytosol of the fungus co-cultured with the methanogen.

Conclusion: The co-culture of the anaerobic fungus and the methanogen is a good model for studying the microbial interaction between H2-producing and H2-utilizing microorganisms. However, metabolism in hydrogenosome needs to be further studied to gain better insight in the hydrogen transfer among microorganisms.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.5713/ajas.19.0649DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7649409PMC
December 2020

Genome-wide analysis of epigenetic and transcriptional changes associated with heterosis in pigeonpea.

Plant Biotechnol J 2020 08 3;18(8):1697-1710. Epub 2020 Feb 3.

International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, India.

Hybrids are extensively used in agriculture to deliver an increase in yield, yet the molecular basis of heterosis is not well understood. Global DNA methylation analysis, transcriptome analysis and small RNA profiling were aimed to understand the epigenetic effect of the changes in gene expression level in the two hybrids and their parental lines. Increased DNA methylation was observed in both the hybrids as compared to their parents. This increased DNA methylation in hybrids showed that majority of the 24-nt siRNA clusters had higher expression in hybrids than the parents. Transcriptome analysis revealed that various phytohormones (auxin and salicylic acid) responsive hybrid-MPV DEGs were significantly altered in both the hybrids in comparison to MPV. DEGs associated with plant immunity and growth were overexpressed whereas DEGs associated with basal defence level were repressed. This antagonistic patterns of gene expression might contribute to the greater growth of the hybrids. It was also noticed that some common as well as unique changes in the regulatory pathways were associated with heterotic growth in both the hybrids. Approximately 70% and 67% of down-regulated hybrid-MPV DEGs were found to be differentially methylated in ICPH 2671 and ICPH 2740 hybrid, respectively. This reflected the association of epigenetic regulation in altered gene expressions. Our findings also revealed that miRNAs might play important roles in hybrid vigour in both the hybrids by regulating their target genes, especially in controlling plant growth and development, defence and stress response pathways. The above finding provides an insight into the molecular mechanism of pigeonpea heterosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/pbi.13333DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7336283PMC
August 2020

Phthalate esters contamination in vegetable-soil system of facility greenhouses in Jingmen, central China and the assessment of health risk.

Environ Geochem Health 2020 Sep 6;42(9):2703-2721. Epub 2020 Jan 6.

Jiangsu Rainfine Environmental Science and Technology Co. Ltd., Nanjing, 210000, China.

Residual levels of six priority phthalate esters (PAEs) compounds in top soils and vegetables of 40 selected greenhouses in two typical facility vegetable greenhouse production areas of Jingmen (Hubei, central China) were determined. Total concentrations of six target PAEs (Σ6PAEs) in 72 vegetable and 40 soil samples ranged from 862 ± 209 to 4260 ± 385 µg kg (dry weight, DW), and from 1122 ± 156 to 4134 ± 272 µg kg (DW), respectively. Di-n-butyl phthalate and di-(2-ethylhexyl) phthalate (DEHP) are the two most frequently and massively detected in all samples. The highest Σ6PAEs appeared in leaf samples of Tuanlin, vegetable fruits of Zhongxiang and soils of Tuanlin. No carcinogenic risk was posed based on the results of health risk assessment, but non-carcinogenic risk of DEHP to children less than 6 years old in Tuanlin (all 24 sampling sites) and Zhongxiang (4/16 sampling sites) and people older than 6 years old in Tuanlin (19/24 sampling sites) were achieved from hazard quotient values. The contamination risk problem of PAEs in Tuanlin deserves greatest concern in Jingmen. Combined with the results of our former study, the health risks of target pollutants were clarified and the lack of survey data on PAE concentrations in facility vegetable greenhouses of central China was filled in. Due to high residuals and significant non-carcinogenic risk values, DEHP should be nominated as priority PAEs in China. Our study suggested better regulation for PAEs control in intensively managed greenhouses and references for revision of Chinese environmental standards.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10653-019-00504-2DOI Listing
September 2020

Facile Approach for Synthesizing High-Performance MnO/C Electrodes from Rice Husk.

ACS Omega 2019 Nov 1;4(20):18908-18917. Epub 2019 Nov 1.

Department of Physical and Environmental Sciences, University of Toronto Scarborough, Toronto M1C 1A4, Canada.

Pyrolysis is an effective way to convert biomass into biofuel while obtaining highly porous active carbon materials. In this study, a facile approach, involving hydrothermal and pyrolysis steps, is described for preparing hybrid metal oxide nanoparticle-embedded porous hard carbon matrices (MnO/C) from the biowaste rice husk and organometallic precursors. It was found that the pyrolysis/calcination temperature had a strong influence over the microstructure, especially over the porosity, but also over the carbon content and crystallinity of the nanocomposites; hence, the electrical properties can be controlled. Galvanostatic measurements showed that the nanocomposite obtained at 600 °C exhibited the highest charge/discharge capacity and best stability, delivering an initial discharge capacity of 1104 mA·h·g at a current density of 200 mA·g, and retaining a value of 830 mA·h·g after 200 cycles, suggesting excellent cycle stability. A discharge capacity of 581 mA·h·g was obtained even at a current density as high as 2400 mA·g, demonstrating superb rate capability. This outstanding electrochemical performance, ascribed to high electrochemical activity of the embedded MnO nanoparticles enhanced by electrical conductivity provided through the high surface area of the active porous carbon support, is discussed in relation to the microstructure of the nanocomposite.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsomega.9b03026DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6854825PMC
November 2019

Regulation of Molecular Chaperone GRP78 by Hepatitis B Virus: Control of Viral Replication and Cell Survival.

Mol Cell Biol 2020 01 16;40(3). Epub 2020 Jan 16.

Department of Immunology, Shanghai Medical College of Fudan University, Shanghai, People's Republic of China

Chronic hepatitis B (CHB) remains a global health problem, carrying a high risk for progression into cirrhosis and liver failure. Molecular chaperones are involved in diverse pathophysiological processes including viral infection. However, the role of molecular chaperones in hepatitis B virus (HBV) infection and its underlying mechanisms remain unclear. Here, we identified GRP78 as one of the molecular chaperones most strongly induced by HBV in human hepatocytes. Gain- and loss-of-function analyses demonstrated that GRP78 exerted an inhibitory effect on HBV transcription and replication. Further study showed that GRP78 was involved in the activation of AKT/mTOR signaling in hepatocytes, which contributed to GRP78-mediated inhibition of HBV. Of note, HBV-upregulated GRP78 was found to play a crucial role in maintaining the survival of hepatocytes via facilitating a mild endoplasmic reticulum (ER) stress. Together, our findings suggest that HBV may sacrifice part of its replication for establishing a persistent infection through induction of GRP78, a master ER stress regulator. Targeting GRP78 may help develop to design novel therapeutic strategies against chronic HBV infection and the associated hepatocellular carcinoma.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1128/MCB.00475-19DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6965036PMC
January 2020

Durable, cost-effective and superhydrophilic chitosan-alginate hydrogel-coated mesh for efficient oil/water separation.

Carbohydr Polym 2019 Dec 5;226:115279. Epub 2019 Sep 5.

College of Ecological Environment and Urban Construction, Fujian University of Technology, Fuzhou 350118, China.

Hydrogels with low-adhesive superoleophobicity are ideal candidates for modifying filtration substrates to achieve efficient and antifouling oil/water separation. However, there are still some unfavorable factors hindering their practical application, including expensive raw materials, complex fabrication process, poor stability and durability. In this work, a durable, cost-effective and superhydrophilic chitosan-alginate (CS-ALG) hydrogel coated mesh was developed by a facile, two-step dip-coating method for efficient oil/water separation in hypersaline environments. By integrating polysaccharide-based superhydrophobic surfaces and the hierarchical micro-/nanostructures, the as-fabricated CS-ALG hydrogel coated mesh exhibits excellent underwater superoleophobicity and anti-oil-fouling performance. Benefiting from that, the mesh could separate various oil/water mixtures with high separation efficiency (> 99%). It is worth mentioning that the double-cross-linked CS-ALG hydrogel based on sequential electrostatic interaction and ionic cross-linking shows excellent durability in hypersaline environments. All these attractive advantages make the hydrogel-coated mesh a promising candidate for oily wastewater treatment and oil spill cleanup.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.carbpol.2019.115279DOI Listing
December 2019

Parallel Synthesis and Screening of Supramolecular Chemosensors That Achieve Fluorescent Turn-on Detection of Drugs in Saliva.

J Am Chem Soc 2019 10 11;141(42):16763-16771. Epub 2019 Oct 11.

Department of Chemistry , University of Victoria , PO Box 3065, STN CSC , Victoria , British Columbia V8W 3 V6 , Canada.

Programming and controlling molecular recognition in aqueous solutions is increasingly common, but creating supramolecular sensors that detect analytes in biologically relevant solutions remains a nontrivial task. We report here a parallel synthesis-driven approach to create a family of self-assembling dimeric sensors that we call DimerDyes and its use for the rapid identification of salt-tolerant sensors for illicit drugs. We developed an efficient method that involves parallel synthesis and screening in crude form without the need to purify each potential sensor. Structurally diverse "hit" DimerDyes were resynthesized and purified and were each shown to assemble into homodimers in water in the programmed way. DimerDyes provided a "turn-on" fluorescence detection of multiple illicit drugs at low micromolar concentrations in water and in saliva. The combination of multiple agents into a sensor array was successfully able to detect and discriminate between closely related drugs and metabolites in multiple important drug families.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/jacs.9b07073DOI Listing
October 2019

Collagen peptides promote photoaging skin cell repair by activating the TGF-β/Smad pathway and depressing collagen degradation.

Food Funct 2019 Sep 9;10(9):6121-6134. Epub 2019 Sep 9.

College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China. and Key Laboratory of Functional Dairy, Ministry of Education, Beijing 100083, China.

Collagen hydrolysate has been widely used as a nutraceutical agent against skin aging and has gained increasing attention. Previous research has suggested that oral administration of antioxidant collagen peptides (ACPs) exerted beneficial effects on the photoaging skin structure and collagen. However, the bioactive components in ACP metabolites that are responsible for the repair effects have not been elucidated. In this study, serum containing collagen peptides (CPS) after oral administration and collagen peptides isolated from serum metabolites (SCP) were collected and their effects on cell proliferation, hyaluronic acid secretion and the collagen synthesis pathway in UVA-induced skin fibroblasts (ESF) were evaluated. Furthermore, hydroxyproline (Hyp)-containing collagen peptides from SCP were analyzed and their repair effects were examined. The repair effects of ACP metabolites in serum differed depending on the preparation method and SCP were the active components responsible for the repair effects. SCP displayed repair effects by activating the TGF-β/Smad pathway to promote procollagen synthesis and suppressing AP-1, MMP-1 and MMP-3 protein expression to prevent collagen degradation, in which SHCP exhibited the strongest bioactivity. In addition, SCP showed repair effects by reactive oxygen species (ROS) scavenging activity and preserving the endogenous antioxidant defense systems. Furthermore, IO (Ile-Hyp) and AOG (Ala-Hyp-Gly) were identified as the active peptides promoting procollagen synthesis by activating the TGF-β/Smad3 pathway. These results may be useful in screening of anti-photoaging factors in metabolites and producing highly efficient collagen peptide products.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9fo00610aDOI Listing
September 2019

Chitosan/ nanofibrillated cellulose aerogel with highly oriented microchannel structure for rapid removal of Pb (II) ions from aqueous solution.

Carbohydr Polym 2019 Nov 2;223:115048. Epub 2019 Jul 2.

College of Ecological Environment and Urban Construction, Fujian University of Technology, Fuzhou 350118, China.

Among the heavy metal ions, Pb (II) can cause serious diseases in the nervous and cardiovascular systems even at an ultralow concentration. Nowadays, various materials with high adsorption capacity have been developed for heavy metal ions adsorption. However, most of them have a low adsorption rate and may take a long time to achieve adsorption equilibrium. In this work, chitosan (CS)/ nanofibrillated cellulose (NFC) aerogel with oriented microchannel structure was developed via a directional freeze-drying approach. The structure of the CS/NFC aerogel was characterized and its adsorption performance for Pb (II) was also investigated. The results reveal that the maximum adsorption capacity for Pb (II) is 252.6 mg g, much higher than most chitosan-based adsorbents reported in the literature. More impressively, the developed aerogel takes only 5 min to achieve 85% of equilibrium adsorption capacity for Pb (II) due to its highly oriented microchannel structure. Furthermore, the aerogel maintains a high removal efficiency (>85%) after 5 adsorption-desorption cycles. The obtained results demonstrate that the as-prepared CS/NFC aerogel can be used as an effective adsorbent for Pb (II) removal from aqueous solution.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.carbpol.2019.115048DOI Listing
November 2019