Publications by authors named "Yupeng Yan"

26 Publications

  • Page 1 of 1

Co-sorption of metal ions and inorganic anions/organic ligands on environmental minerals: A review.

Sci Total Environ 2021 Aug 26;803:149918. Epub 2021 Aug 26.

Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture and Rural Affairs, College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, People's Republic of China. Electronic address:

Co-sorption of metal ions and anions/ligands at the mineral-water interface plays a critical role in regulating the mobility, transport, fate, and bioavailability of these components in natural environments. This review focuses on co-sorption of metal ions and naturally occurring anions/ligands on environmentally relevant minerals. The underlying mechanisms for their interfacial reactions are summarized and the environmental impacts are discussed. Co-sorption mechanisms of these components depend on a variety of factors, such as the identity and properties of minerals, pH, species and concentration of metal ions and anions/ligands, addition sequence of co-sorbed ions, and reaction time. The simultaneous presence of metal ions and anions/ligands alters the initial sorption behaviors with promotive or competitive effects. Promotive effects are mainly attributed to surface electrostatic interactions, ternary surface complexation, and surface precipitation, especially for the co-sorption systems of metal ions and inorganic anions on minerals. Competitive effects involve potential complexation of metal-anions/ligands in solution or their competition for surface adsorption sites. Organic ligands usually increase metal ion sorption on minerals at low pH via forming ternary surface complexes or surface precipitates, but inhibit metal ion sorption via the formation of aqueous complexes at high pH. The different mechanisms may act simultaneously during metal ion and anion/ligand co-sorption on minerals. Finally, the potential application for remediation of metal-contaminated sites is discussed based on the different co-sorption behaviors. Future challenges and topics are raised for metal-anion/ligand co-sorption research.
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http://dx.doi.org/10.1016/j.scitotenv.2021.149918DOI Listing
August 2021

Low (thyroid hormone receptor beta) Promoter Methylation Levels in Peripheral Blood Leukocytes Induced By Systematic Inflammation Are Involved in Low Thyroid Hormone Function in Metabolic Syndrome.

Hypertension 2021 Sep 15;78(4):1005-1015. Epub 2021 Aug 15.

State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing (B.C., X.X., Jin'e Wang, H.W., C.W., Y.Y., Y. Zong, Y. Zhang, R.H., A.M.G., Y.W.).

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http://dx.doi.org/10.1161/HYPERTENSIONAHA.121.17847DOI Listing
September 2021

Kinetics of Mn(II) adsorption and catalytic oxidation on the surface of ferrihydrite.

Sci Total Environ 2021 Oct 4;791:148225. Epub 2021 Jun 4.

Key Laboratory of Poyang Lake Basin Agricultural Resource and Ecology of Jiangxi Province, College of Land Resource and Environment, Jiangxi Agricultural University, Nanchang 330045, China. Electronic address:

Mn(II) adsorption-oxidation on iron (Fe) oxides (e.g., ferrihydrite) occurs in various soils and sediments, significantly affecting the toxicities and bioavailabilities of Mn and other associated elements. However, the detailed processes of Mn(II) adsorption-oxidation on ferrihydrite remain elusive. In this study, the Mn(II) (2 mM) adsorption-oxidation kinetics on different masses of ferrihydrite (0.25, 0.50, 1.00, and 1.25 g) at pH 7 were determined using batch kinetic studies combined with X-ray diffraction, transmission electron microscopy, and wet chemistry analyses. The results indicated that the low-concentration Mn(II) adsorption-oxidation on ferrihydrite occurred in two steps. First, Mn(II) was adsorbed onto ferrihydrite, where it was partially oxidized by the catalytic effect of ferrihydrite, within ~0-60 min; subsequently, the remaining Mn(II) underwent autocatalytic oxidation on the previously generated Mn (oxyhydr)oxides. The initial adsorption-oxidation behaviors of Mn(II) on the ferrihydrite surface determined the kinetics of Mn(II) removal and oxidation, and therefore the amounts and types of Mn (oxyhydr)oxides formed. Furthermore, the specific characteristics of Mn(II) adsorption-oxidation on ferrihydrite showed a strong dependence on the Fe/Mn molar ratio. When this ratio was below 16.35, the initial process was dominated by Mn(II) adsorption onto ferrihydrite, with slight oxidation generating hausmannite (~0-60 min), followed by the catalytic oxidation of Mn(II) on the formed hausmannite, generating manganite or groutite. Conversely, when the Fe/Mn molar ratio was above 32.7, the reactions primarily involved Mn(II) adsorption onto ferrihydrite with minor oxidation to form Mn(III/IV) (oxyhydr)oxides (~0-60 min), followed by the autocatalytic oxidation of Mn(II) on the freshly-generated Mn(III/IV) (oxyhydr)oxides, forming Mn(III) (oxyhydr)oxides, i.e., feitknechtite. These results provide further insight into the interaction between Fe and Mn, Mn(II) removal, and Mn (oxyhydr)oxide formation in the environment.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148225DOI Listing
October 2021

Effects of miR‑210‑3p on the erythroid differentiation of K562 cells under hypoxia.

Mol Med Rep 2021 Aug 10;24(2). Epub 2021 Jun 10.

Department of Biochemistry, Qing Hai University Medical College, Xining, Qinghai 810000, P.R. China.

GATA binding protein 1 (GATA‑1) is one of the most important hematopoietic transcription factors in the production of blood cells, such as platelets, eosinophils, mast cells and erythrocytes. GATA‑1 regulates the participation of microRNA (miRNAs/miRs) in erythroid differentiation under normoxia. However, GATA‑1 expression and the regulation of miR‑210‑3p in the context of erythroid differentiation under hypoxia remain unknown. The present study examined the expression levels of GATA‑1 and miR‑210‑3p in the model of erythroid differentiation in K562 cells under hypoxia, and determined the effects of GATA‑1, miR‑210‑3p and SMAD2 on erythroid differentiation through lentivirus transfection experiments. The present study detected increased GATA‑1 expression under hypoxia. Moreover, miR‑210‑3p was identified as a positive regulator of erythroid differentiation, which was upregulated both during erythroid differentiation and in GATA‑1 overexpression experiments under hypoxia. Importantly, in the K562 cell model of erythroid differentiation under hypoxia, miR‑210‑3p was upregulated in a GATA‑1‑dependent manner. Using a double luciferase reporter assay, miR‑210‑3p was identified as a downstream target of GATA‑1‑mediated regulation of erythropoiesis. Gain‑ or loss‑of‑function analysis of miR‑210‑3p identified its importance in erythroid differentiation. Furthermore, it was found that SMAD2 may be a downstream target gene for miR‑210‑3p. Bioinformatics predictions suggested that SMAD2 mediated miR‑210‑3p‑induced regulation of erythroid differentiation. Collectively, the present study provides novel insights into the miRNA regulation of erythroid differentiation.
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http://dx.doi.org/10.3892/mmr.2021.12202DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8201459PMC
August 2021

Musk ketone induces apoptosis of gastric cancer cells via downregulation of sorbin and SH3 domain containing 2.

Mol Med Rep 2021 06 21;23(6). Epub 2021 Apr 21.

Department of Basic Medical Sciences, Qinghai University Medical College, Xining, Qinghai 810016, P.R. China.

Musk ketone exerts antiproliferative effects on several types of cancer, such as lung and breast cancer. However, the effects and underlying mechanisms of action of musk ketone in gastric cancer (GC) are poorly understood. The present study aimed to investigate the effects of musk ketone in GC cells. The present study indicated that musk ketone exerted significant anticancer effects on GC cells. The IC values of musk ketone were 4.2 and 10.06 M in AGS and HGC‑27 cells, respectively. Low dosage of musk ketone significantly suppressed the proliferation and colony formation of AGS and HGC‑27 cells. Cell cycle arrest and apoptosis were induced by musk ketone. Furthermore, microarray data indicated that musk ketone treatment led to downregulation of various genes, including sorbin and SH3 domain containing 2 (SORBS2). Reverse transcription‑quantitative PCR and immunoblotting results indicated that musk ketone repressed mRNA and protein expression levels of SORBS2. It was also shown that knockdown of SORBS2 inhibited the proliferation and colony formation of HGC‑27 cells. The antiproliferative effects of musk ketone were decreased in HGC‑27 cells with SORBS2 silencing. In summary, the present study indicated that musk ketone suppressed the proliferation and growth of GC partly by downregulating SORBS2 expression.
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http://dx.doi.org/10.3892/mmr.2021.12089DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8060797PMC
June 2021

Highly enhanced oxidation of arsenite at the surface of birnessite in the presence of pyrophosphate and the underlying reaction mechanisms.

Water Res 2020 Dec 10;187:116420. Epub 2020 Sep 10.

Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture, College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, China. Electronic address:

Manganese(IV) oxides, and more especially birnessite, rank among the most efficient metal oxides for As(III) oxidation and subsequent sorption, and thus for arsenic immobilization. Efficiency is limited however by the precipitation of low valence Mn (hydr)oxides at the birnessite surface that leads to its passivation. The present work investigates experimentally the influence of chelating agents on this oxidative process. Specifically, the influence of sodium pyrophosphate (PP), an efficient Mn(III) chelating agent, on As(III) oxidation by birnessite was investigated using batch experiments and different arsenic concentrations at circum-neutral pH. In the absence of PP, Mn(II/III) species are continuously generated during As(III) oxidation and adsorbed to the mineral surface. Field emission-scanning electron microscopy, synchrotron-based X-ray diffraction and Fourier transform infrared spectroscopy indicate that manganite is formed, passivating birnessite surface and thus hampering the oxidative process. In the presence of PP, generated Mn(II/III) species form soluble complexes, thus inhibiting surface passivation and promoting As(III) conversion to As(V) with PP. Enhancement of As(III) oxidation by Mn oxides strongly depends on the affinity of the chelating agent for Mn(III) and from the induced stability of Mn(III) complexes. Compared to PP, the positive influence of oxalate, for example, on the oxidative process is more limited. The present study thus provides new insights into the possible optimization of arsenic removal from water using Mn oxides, and on the possible environmental control of arsenic contamination by these ubiquitous nontoxic mineral species.
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http://dx.doi.org/10.1016/j.watres.2020.116420DOI Listing
December 2020

Quantitative investigation of ZnO nanoparticle dissolution in the presence of δ-MnO.

Environ Sci Pollut Res Int 2020 May 13;27(13):14751-14762. Epub 2020 Feb 13.

Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture, College of Resources and Environment, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.

The widespread use of zinc oxide nanoparticles (ZnO NPs), the second most produced nanomaterial, inevitably leads to their release into the environment. In this study, dissolution and transformation of ZnO NPs in the presence of δ-MnO, an abundant and ubiquitous manganese (Mn) oxide mineral, was investigated via a suite of techniques covering bulk to molecular scales. Dissolution kinetics indicated that the presence of δ-MnO significantly affected ZnO NP dissolution rate/trend and equilibrium Zn concentration, which were found to be mainly dependent on the concentration and mass ratio of ZnO NPs and δ-MnO. Approximately 300 mg ZnO NPs per g δ-MnO was expected for ZnO NP uptake at pH 7.0 via ZnO NP dissolution and surface Zn adsorption. X-ray diffraction (XRD), ζ potential, high-resolution transmission electron microscopy (HR-TEM), and Zn K-edge X-ray absorption spectroscopy (XAS) results revealed that when the mole content of ZnO NPs was less than the total adsorption sites of δ-MnO surface, ZnO NPs were completely dissolved and adsorbed on δ-MnO surface in the form of inner-sphere complexes. A fraction of ZnO NPs persisted when the mole ratio of ZnO to δ-MnO further increased. These results suggest that the transformation and fate of ZnO NPs is affected by environment-relevant minerals such as Mn oxides due to their huge capacity of fixing dissolved metal cations at the surface or interlayer structure.
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http://dx.doi.org/10.1007/s11356-020-07965-4DOI Listing
May 2020

Fate of atrazine and its relationship with environmental factors in distinctly different lake sediments associated with hydrophytes.

Environ Pollut 2020 Jan 11;256:113371. Epub 2019 Oct 11.

College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, China.

Atrazine contamination is of great concern due to its widespread occurrence in shallow lakes. Here, the distribution and degradation of atrazine in acidic and alkaline lake systems were investigated. Meanwhile, the bacterial communities in different sediments and the effects of environmental factors on atrazine-degrading bacteria were evaluated. In the lake systems without plants, atrazine levels in sediment interstitial water reached peak concentrations on the 4th d. More than 90% of atrazine was then degraded in all sediment interstitial water by day 30. Meanwhile, the degradation rate of atrazine in alkaline sediments was faster than that in acidic sediments. Values of hydroxylated metabolites in the acidic lake sediments tended to be greater. Moreover, the amounts of Proteobacteria, Actinobacteria, Firmicute, Nitrospinae, Aminicenantes, Ignavibacteriae and Saccharibacteria in acidic Tangxunhu Lake sediments were significantly different from alkaline Honghu Lake sediments, while the amounts of Cyanobacteria and Saccharibacteria in sediments treated with atrazine were significantly greater than those in sediments without atrazine (P < 0.05). Notably, pH was the most relevant environmental factor in the quantitative variation of atrazine-degrading bacteria, including in Clostridium-sensu-stricto, Pseudomonas, Comamonas and Rhodobacter. The Mantel test results indicated that the degradation of atrazine in different sediments was mainly affected by the sediment physicochemical properties rather than by the addition of atrazine and the cultivation of hydrophytes.
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http://dx.doi.org/10.1016/j.envpol.2019.113371DOI Listing
January 2020

Effects of myo-inositol hexakisphosphate, ferrihydrite coating, ionic strength and pH on the transport of TiO nanoparticles in quartz sand.

Environ Pollut 2019 Sep 4;252(Pt B):1193-1201. Epub 2019 Jun 4.

Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture, College of Resources and Environment, Huazhong Agriculture University, Wuhan, 430070, China. Electronic address:

Evaluating the fate and transport of nanoparticles (NPs) in the subsurface environment is critical for predicting the potential risks to both of the human health and environmental safety. It is believed that numerous environmental factors conspire to control the transport dynamics of nanoparticles, yet the effects of organic phosphates on nanoparticles transport remain largely unknown. In this work, we quantified the transport process of TiO nanoparticle (nTiO) and their retention patterns in water-saturated sand columns under various myo-inositol hexakisphosphate (IHP) or phosphate (Pi) concentrations (0-180 μM P), ferrihydrite coating fractions (λ, 0-30%), ionic strengths (1-50 mM KCl), and pH values (4-8). The transport of nTiO was enhanced at increased P concentration due to the enhanced colloidal stability. As compared with Pi at the equivalent P level, IHP showed stronger effect on the electrokinetic properties of nTiO particles due to its relatively more negative charge and higher adsorption affinity, thereby facilitating the nTiO transport (and thus reduced retention) in porous media. At the IHP concentration of 5 μM, the retention of nTiO increased with increasing λ and ionic strength, while decreased with pH. In addition, the retention profiles of nTiO showed a typical hyperexponential pattern for most scenarios mainly due to the unfavorable attachment, and can be well described by a hybrid mathematical model that coupled convection dispersion equations with a two-site kinetic model and DLVO theory. These quantitative estimations revealed the importance of IHP on affecting the transport of nTiO typically in phosphorus-enriched environments. It provides new insights into advanced understanding of the co-transport of nanoparticles and phosphorus in natural systems, essential for both nanoparticle exposure and water eutrophication.
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http://dx.doi.org/10.1016/j.envpol.2019.06.008DOI Listing
September 2019

High prevalence of KRAS/BRAF somatic mutations in brain and spinal cord arteriovenous malformations.

Brain 2019 01;142(1):23-34

State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Brain and spinal arteriovenous malformations are congenital lesions causing intracranial haemorrhage or permanent disability especially in young people. We investigated whether the vast majority or all brain and spinal arteriovenous malformations are associated with detectable tumour-related somatic mutations. In a cohort of 31 patients (21 with brain and 10 with spinal arteriovenous malformations), tissue and paired blood samples were analysed with ultradeep next generation sequencing of a panel of 422 common tumour genes to identify the somatic mutations. We used droplet digital polymerase chain reaction to confirm the panel sequenced mutations and identify the additional low variant frequency mutations. The association of mutation variant frequencies and clinical features were analysed. The average sequencing depth was 1077 ± 298×. High prevalence (87.1%) of KRAS/BRAF somatic mutations was found in brain and spinal arteriovenous malformations with no other replicated tumour-related mutations. The prevalence of KRAS/BRAF mutation was 81.0% (17 of 21) in brain and 100% (10 of 10) in spinal arteriovenous malformations. We detected activating BRAF mutations and two novel mutations in KRAS (p.G12A and p.S65_A66insDS) in CNS arteriovenous malformations for the first time. The mutation variant frequencies were negatively correlated with nidus volumes of brain (P = 0.038) and spinal (P = 0.028) arteriovenous malformations but not ages. Our findings support a causative role of somatic tumour-related mutations of KRAS/BRAF in the overwhelming majority of brain and spinal arteriovenous malformations. This pathway homogeneity and high prevalence implies the development of targeted therapies with RAS/RAF pathway inhibitors without the necessity of tissue genetic diagnosis.10.1093/brain/awy307_video1awy307media15978667388001.
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http://dx.doi.org/10.1093/brain/awy307DOI Listing
January 2019

Formation of Zn-Al layered double hydroxides (LDH) during the interaction of ZnO nanoparticles (NPs) with γ-AlO.

Sci Total Environ 2019 Feb 19;650(Pt 2):1980-1987. Epub 2018 Sep 19.

Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture, College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, China. Electronic address:

Zinc and aluminum layered double hydroxides (Zn-Al LDH) are a common group of major Zn species in various Zn-contaminated soil/sediment environments, yet their formation pathways and underlying mechanisms under varied conditions are not well understood. This study investigated the formation of Zn-Al LDHs through the direct interaction of two solid substrates, ZnO nanoparticles (NPs) and a representative Al oxide, γ-AlO. Batch experiments and complementary microscopic and spectroscopic analyses were conducted to elucidate the reaction kinetics and mechanisms, as well as the morphologic and structural evolution of the products. Dissolved Zn and Al concentrations decreased significantly in a dual solid system compared to a single solid system. A bulk Zn-Al LDH phase was found to form under a wide pH range (6.5-9.5). Aside from Zn-Al LDH, γ-AlO was the main remaining solid phase at pH 6.5, whereas ZnO NPs were the main residual solid phases at pH 9.5. Formation of amorphous Zn(OH) was also observed at both pH values, likely due to Zn release at low pH and Al(OH) adsorption at high pH. It is proposed that the formation of Zn-Al LDH occurs via a dissolution-sorption-coprecipitation process, where the solubility of ZnO NPs or γ-AlO solid phases determines the reaction pathways and kinetics under varied pH conditions. The results from this work revealed the transformation mechanisms for ZnO NPs under conditions from weakly acidic to alkaline pH with highly available Al particles and shed light on the environmental fate of ZnO NPs in Zn or ZnO NP contaminated environments.
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http://dx.doi.org/10.1016/j.scitotenv.2018.09.230DOI Listing
February 2019

Phytoextraction and biodegradation of atrazine by Myriophyllum spicatum and evaluation of bacterial communities involved in atrazine degradation in lake sediment.

Chemosphere 2018 Oct 14;209:439-448. Epub 2018 Jun 14.

College of Resources and Environment, Huazhong Agricultural University, Wuhan, 430070, China. Electronic address:

The accumulation of atrazine in lake sediments leads to persistent contamination, which may damage the succeeding submerged plants and create potential threats to the lake eco-environment. In this study, the degradation characteristics of atrazine and its detoxication by Myriophyllum spicatum and the associated bacterial community in lake sediments were evaluated. M. spicatum absorbed more than 18-fold the amount of atrazine in sediments and degraded atrazine to hydroxyatrazine (HA), deelthylatrazine (DEA), didealkylatrazine (DDA), cyanuric acid (CYA) and biuret. The formation of biuret suggested for the first time, the ring opening of atrazine in an aquatic plant. The residual rate of atrazine was 6.5 ± 2.0% in M. spicatum-grown sediment, which was significantly lower than the 18.0 ± 2.5% in unplanted sediments on day 60 (P < 0.05). Moreover, on day 15, the increase in contents of HA, CYA and biuret in M. spicatum-grown sediment indicated that M. spicatum promoted the degradation and removal of atrazine following rapid dechlorination. The colonization of M. spicatum and the addition of atrazine altered the structure of the dominant bacterial community in sediments, including effects on Nitrospirae and Acidobacteria. Based on the maximum amount among the genera of atrazine-degrading bacteria, Acetobacter was most likely responsible for the degradation of atrazine. Our findings reveal the natural attenuation of atrazine by aquatic organisms.
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http://dx.doi.org/10.1016/j.chemosphere.2018.06.055DOI Listing
October 2018

Catalytic oxidation of arsenite and reaction pathways on the surface of CuO nanoparticles at a wide range of pHs.

Geochem Trans 2018 Jun 22;19(1):12. Epub 2018 Jun 22.

Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture College of Resources and Environment, Huazhong Agricultural University, Wuhan, 430070, China.

Recently, the wide application of CuO nanoparticles (NPs) in engineering field inevitably leads to its release into various geologic settings, which has aroused great concern about the geochemical behaviors of CuO NPs due to its high surface reactivity and impact on the fate of co-existing contaminants. However, the redox transformation of pollutants mediated by CuO NPs and the underlying mechanism still remain poorly understood. Here, we studied the interaction of CuO NPs with As(III), and explored the reaction pathways using batch experiments and multiple spectroscopic techniques. The results of in situ quick scanning X-ray absorption spectroscopy (Q-XAS) analysis verified that CuO NPs is capable of catalytically oxidize As(III) under dark conditions efficiently at a wide range of pHs. As(III) was firstly adsorbed on CuO NPs surface and then gradually oxidized to As(V) with dissolved O as the terminal electron acceptor. As(III) adsorption increased to the maximum at a pH close to PZC of CuO NPs (~ pH 9.2), and then sharply decreased with increasing pH, while the oxidation capacity monotonically increased with pH. X-ray photoelectron spectroscopy and electron paramagnetic resonance characterization of samples from batch experiments indicated that two pathways may be involved in As(III) catalytic oxidation: (1) direct electron transfer from As(III) to Cu(II), followed by concomitant re-oxidation of the produced Cu(I) by dissolved O back to Cu(II) on CuO NPs surface, and (2) As(III) oxidation by reactive oxygen species (ROS) produced from the above Cu(I) oxygenation process. These observations facilitate a better understanding of the surface catalytic property of CuO NPs and its interaction with As(III) and other elements with variable valence in geochemical environments.
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http://dx.doi.org/10.1186/s12932-018-0058-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6014938PMC
June 2018

Hyperbolic accelerating beams and their relation with Hermite-Gaussian beams.

J Opt Soc Am A Opt Image Sci Vis 2018 Feb;35(2):262-266

We derive the initial distributions of phase and complex amplitude of accelerating beams with arbitrary predesigned hyperbolic trajectories using the caustic-design method and explore the relation between these beams and Hermite-Gaussian beams. The results show the hyperbolic accelerating beams are a larger class of beams than Hermite-Gaussian beams. When the bending parameter is an integer, the hyperbolic accelerating beams have a similar initial complex amplitude distribution and almost the same propagating characteristics as Hermite-Gaussian beams. Through the analysis of the ray-based method, we also derive an approximate expression for the initial complex amplitude of Hermite-Gaussian beams after introducing an amplitude distribution function. Although the proposed approximate expressions of complex amplitude are more complex than the usually used Hermite-Gaussian function, they explicitly indicate the information on local amplitude, wave vector, and internal ray structure (including caustics) of these beams and thus provide us clearer geometrical insights into these beams.
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http://dx.doi.org/10.1364/JOSAA.35.000262DOI Listing
February 2018

NOD receptor and TLR9 modulation in severe acute pancreatitis‑induced intestinal injury.

Mol Med Rep 2017 Dec 29;16(6):8471-8476. Epub 2017 Sep 29.

Intensive Care Unit, China Meitan General Hospital, Beijing 100028, P.R. China.

Severe acute pancreatitis (SAP) has a rapid onset and may cause multiple organ dysfunction syndrome (MODS), which has high mortality. Nucleotide binding oligomerization domain (NOD) receptor and Toll‑like receptor 9 (TLR9), a pattern recognition receptor in innate immunity, are involved in inflammation, immunity and pathogen recognition. The role and mechanism of the NOD receptor and TLR9 in early MODS of SAP‑induced intestinal injury, however, remain unclear. Wistar rats were divided into control, SAP, TLR9 inhibitor and NOD receptor activation groups. Reverse transcription‑quantitative polymerase chain reaction was used to analyze the expression of TLR9, NOD1 and NOD2 in the experimental treatment groups. Serum amylase, creatinine and alanine aminotransferase indices were measured, ELISA was used to determine the expression of tumor necrosis factor‑α (TNF‑α) and interleukin‑1β (IL‑1β) and western blot analysis was used to assess nuclear factor (NF)‑κB expression levels in intestinal tissues. Reactive oxygen species (ROS) levels and superoxide dismutase (SOD) activity were quantified by spectrometry. SAP and NOD receptor activation groups exhibited significantly elevated TLR9, NOD1, NOD2, TNF‑α, IL‑1β and nuclear factor (NF)‑κB levels compared with the control group. Furthermore, ROS production was increased, SOD activity was decreased and higher serum indices were exhibited, compared with the control group. The NOD receptor group presented more significant differences compared with the SAP group. The TLR9 inhibitor group exhibited opposite effects, with markedly decreased TLR9, NOD1, NOD2, TNF‑α, IL‑1β and NF‑κB levels. The TLR9 inhibitor group also presented reduced ROS production, increased SOD activity and lower serum indexes compared to the SAP group. The present study therefore indicated that NOD receptor and TLR9 may modulate the inflammatory response and further impact upon intestinal injury of SAP, via the regulation of NF‑κB expression and the oxidation/antioxidation balance, suggesting therapeutically targeting NOD receptor and TLR9 might be a useful approach for the treatment of severe acute pancreatitis.
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http://dx.doi.org/10.3892/mmr.2017.7661DOI Listing
December 2017

Baf60b-mediated ATM-p53 activation blocks cell identity conversion by sensing chromatin opening.

Cell Res 2017 May 17;27(5):642-656. Epub 2017 Mar 17.

State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.

Lineage conversion by expression of lineage-specific transcription factors is a process of epigenetic remodeling that has low efficiency. The mechanism by which a cell resists lineage conversion is largely unknown. Using hepatic-specific transcription factors Foxa3, Hnf1α and Gata4 (3TF) to induce hepatic conversion in mouse fibroblasts, we showed that 3TF induced strong activation of the ATM-p53 pathway, which led to proliferation arrest and cell death, and it further prevented hepatic conversion. Notably, ATM activation, independent of DNA damage, responded to chromatin opening during hepatic conversion. By characterizing the early molecular events during hepatic conversion, we found that Baf60b, a member of the SWI/SNF chromatin remodeling complex, links chromatin opening to ATM activation by facilitating ATM recruitment to the open chromatin regions of a panel of hepatic gene loci. These findings shed light on cellular responses to lineage conversion by revealing a function of the ATM-p53 pathway in sensing chromatin opening.
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http://dx.doi.org/10.1038/cr.2017.36DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5520852PMC
May 2017

Effects of polyphosphates and orthophosphate on the dissolution and transformation of ZnO nanoparticles.

Chemosphere 2017 Jun 27;176:255-265. Epub 2017 Feb 27.

Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture, College of Resources and Environment, Huazhong Agricultural University, Wuhan, 430070, China. Electronic address:

The fate and toxicity of zinc oxide nanoparticles (ZnO NPs) in nature are affected by solution chemistry such as pH, anions, and natural organic matter (NOM). Inorganic polyphosphates are environmentally ubiquitous phosphorus (P) species that may change the speciation and environmental fate of ZnO NPs. In this study, the interactions of polyphosphates with ZnO NPs and the impacts on ZnO NP dissolution and transformation were investigated and compared with orthophosphate (P). The results revealed that pyrophosphate (P), tripolyphosphate (P), and hexametaphosphate (P) enhanced whereas P inhibited the dissolution of ZnO NPs. In addition, P, P, and P promoted the transformation of ZnO NPs into zinc phosphate (Zn-P) precipitates via interactions with dissolved Zn. However, P-promoted ZnO NP dissolution was through the formation of soluble Zn-P complexes due to the strong capability of P to chelate with Zn. The transformation of ZnO NPs in the presence of P was affected by reaction time, pH, and P/Zn molar ratio. P first formed inner-sphere surface complexes on ZnO NPs, which gradually transformed into crystalline ZnHPO(HO) precipitates. This study provided a new perspective for understanding the reactivity of various forms of inorganic phosphate species with ZnO NPs in the natural environment.
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http://dx.doi.org/10.1016/j.chemosphere.2017.02.134DOI Listing
June 2017

Novel FBN1 mutations are responsible for cardiovascular manifestations of Marfan syndrome.

Mol Biol Rep 2016 Nov 24;43(11):1227-1232. Epub 2016 Aug 24.

State Key Laboratory of Cardiovascular Disease, Sino-German Laboratory for Molecular Medicine, National Center for Cardiovascular Diseases, Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 167 Beilishi Rd, Beijing, 100037, China.

The fibrillin-1 (FBN1) gene mutations result in Marfan syndrome (MFS) and have a variety of phenotypic variations. This disease is involved in the skeletal, ocular and cardiovascular system. Here we analyzed genotype-phenotype correlation in two Chinese families with MFS. Two patients with thoracic aortic aneurysms and dissections were diagnosed as MFS according to the revised Ghent criteria. Peripheral blood samples were collected and genomic DNAs were isolated from available cases, namely, patient-1 and his daughter and son, and patient-2 and his parents. According to the next-generation sequencing results, the mutations in FBN1 were confirmed by direct sequencing. A heterozygous frameshift mutation in exon 12 of FBN1 was found in the proband-1 and his daughter. They showed cardiovascular phenotype thoracic aortic aneurysms and dissections, a life-threatening vascular disease, and atrial septal defect respectively. One de novo missense mutation in exon 50 of FBN1 was identified only in the patient-2, showing aortic root aneurysm and aortic root dilatation. Intriguingly, two novel mutations mainly caused the cardiovascular complications in affected family members. No meaningful mutations were found in these two patients by screening all exons of 428 genes related with cardiovascular disease. The high incidence of cardiovascular manifestations might be associated with the two novel mutations in exon 12 and 50 of FBN1.
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http://dx.doi.org/10.1007/s11033-016-4067-yDOI Listing
November 2016

Enhanced Dissolution and Transformation of ZnO Nanoparticles: The Role of Inositol Hexakisphosphate.

Environ Sci Technol 2016 06 17;50(11):5651-60. Epub 2016 May 17.

Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture, College of Resources and Environment, Huazhong Agricultural University , Wuhan 430070, People's Republic of China.

The toxicity, reactivity, and behavior of zinc oxide (ZnO) nanoparticles (NPs) released in the environment are highly dependent on environmental conditions. Myo-inositol hexakisphosphate (IHP), a common organic phosphate, may interact with NPs and generate new transformation products. In this study, the role of IHP in mediating the dissolution and transformation of ZnO NPs was investigated in the laboratory kinetic experiments using powder X-ray diffraction, attenuated total reflectance Fourier transform infrared spectroscopy, (31)P nuclear magnetic resonance spectroscopy, high-resolution transmission electronic microscopy, and synchrotron-based extended X-ray absorption fine structure spectroscopy. The results indicate that IHP shows a dissolution-precipitation effect, which is different from citrate and EDTA that only enhances Zn dissolution. The enhanced dissolution and transformation of ZnO NPs by IHP (<0.5 h) is found to be strikingly faster than that induced by inorganic phosphate (Pi, > 3.0 h) at pH 7.0, and the reaction rate increases with decreasing pH and increasing IHP concentration. Multitechnique analyses reveal that interaction of ZnO NPs with IHP induces rapid transformation of ZnO NPs into zinc phytate complexes initially and poorly crystalline zinc phytate-like (Zn-IHP) phase finally. Additionally, ZnO NPs preferentially react with IHP and transform to Zn-IHP when Pi and IHP concurrently coexist in a system. Overall, results from this study contribute to an improved understanding of the role of organic phosphates (e.g., IHP) in the speciation and structural transformation of ZnO NPs, which can be leveraged for remediation of ZnO-polluted water and soils.
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http://dx.doi.org/10.1021/acs.est.6b00268DOI Listing
June 2016

Improved survival of porcine acute liver failure by a bioartificial liver device implanted with induced human functional hepatocytes.

Cell Res 2016 Feb 15;26(2):206-16. Epub 2016 Jan 15.

State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academic of Sciences, Shanghai 200031, China.

Acute liver failure (ALF) is a life-threatening illness. The extracorporeal cell-based bioartificial liver (BAL) system could bridge liver transplantation and facilitate liver regeneration for ALF patients by providing metabolic detoxification and synthetic functions. Previous BAL systems, based on hepatoma cells and non-human hepatocytes, achieved limited clinical advances, largely due to poor hepatic functions, cumbersome preparation or safety concerns of these cells. We previously generated human functional hepatocytes by lineage conversion (hiHeps). Here, by improving functional maturity of hiHeps and producing hiHeps at clinical scales (3 billion cells), we developed a hiHep-based BAL system (hiHep-BAL). In a porcine ALF model, hiHep-BAL treatment restored liver functions, corrected blood levels of ammonia and bilirubin, and prolonged survival. Importantly, human albumin and α-1-antitrypsin were detectable in hiHep-BAL-treated ALF pigs. Moreover, hiHep-BAL treatment led to attenuated liver damage, resolved inflammation and enhanced liver regeneration. Our findings indicate a promising clinical application of the hiHep-BAL system.
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http://dx.doi.org/10.1038/cr.2016.6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4746613PMC
February 2016

Surface speciation of myo-inositol hexakisphosphate adsorbed on TiO2 nanoparticles and its impact on their colloidal stability in aqueous suspension: A comparative study with orthophosphate.

Sci Total Environ 2016 Feb 3;544:134-42. Epub 2015 Dec 3.

Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture, College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, China. Electronic address:

Despite extensive research demonstrating the influence of organic matter and inorganic phosphate on the stability of TiO2 nanoparticles (NPs), far less research has assessed the impact of myo-inositol hexakisphosphate (IHP), a common organic phosphate widely present in the environment. In this study, the adsorption of IHP on TiO2 NPs and its impact on their colloidal stability were investigated using batch experiments, dynamic light scattering (DLS) techniques, in situ attenuated total reflectance Fourier transform infrared (ATR-FTIR) and solid-state (31)P nuclear magnetic resonance (NMR) spectroscopy. Inorganic orthophosphate (Pi) adsorption was run for comparison. The ratio of the Pi/IHP adsorption density (1.528: 0.453) at pH5.0 suggested that IHP may complex on the TiO2 surface through three of its six phosphate groups. Zeta potential measurements, ATR-FTIR and NMR spectra indicated that IHP/Pi adsorbed onto TiO2 NPs by forming inner-sphere complexes and modified the surface charge of these NPs, which exerted a great impact on their colloidal stability. Interactions between NPs measured by sedimentation and aggregation size highly depended on the pH, surface phosphorus coverage, and surface phosphorus species. The impact of IHP on the aggregation and dispersion of TiO2 NPs was significantly larger than that of Pi, in agreement with the calculation from the DLVO theory. This study highlighted the impact of IHP relative to Pi on the colloidal stability of TiO2 NPs in phosphorus-enriched environments.
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http://dx.doi.org/10.1016/j.scitotenv.2015.11.157DOI Listing
February 2016

Size-dependent sorption of myo-inositol hexakisphosphate and orthophosphate on nano-γ-Al2O3.

J Colloid Interface Sci 2015 Aug 9;451:85-92. Epub 2015 Apr 9.

Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture, College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, People's Republic of China. Electronic address:

The effects of particle size (5, 35 and 70nm) on the sorption of myo-inositol hexakisphosphate (IHP) and inorganic phosphate (KH2PO4, Pi) on γ-Al2O3 nanoparticles were investigated using batch sorption experiments, zeta potential measurements and solid-state nuclear magnetic resonance spectroscopy (NMR). The results show that the maximum sorption densities (μmolm(-2)) for IHP and Pi increase with decreasing γ-Al2O3 particle size. The sorption affinity of γ-Al2O3 for IHP and Pi generally increases with decreasing particle size, and the sorption affinity for IHP is approximately one order of magnitude greater than that for Pi. In our experimental time scale, surface complexation is the main mechanism for IHP and Pi sorption on large size γ-Al2O3. While an additional surface precipitation mechanism, indicated by solid-state (31)P and (27)Al NMR data, is partly responsible for the greater sorption density on very small size γ-Al2O3. Compared with Pi, the effect of particle size on the sorption of IHP is more pronounced. The results suggest a size-dependent surface reactivity of Al2O3 nanoparticles with Pi/IHP. The underlying mechanism will also be relevant for other small nanosize (hydr)oxide particles and is important for our understanding of the role of small nanoparticles in controlling the mobility and fate of organic and inorganic phosphates in the environment.
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http://dx.doi.org/10.1016/j.jcis.2015.03.045DOI Listing
August 2015

Mechanism of myo-inositol hexakisphosphate sorption on amorphous aluminum hydroxide: spectroscopic evidence for rapid surface precipitation.

Environ Sci Technol 2014 Jun 29;48(12):6735-42. Epub 2014 May 29.

Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture, College of Resources and Environment, Huazhong Agricultural University , Wuhan 430070, People's Republic of China.

Inositol hexakisphosphates are the most abundant organic phosphates (OPs) in most soils and sediments. Adsorption, desorption, and precipitation reactions at environmental interfaces govern the reactivity, speciation, mobility, and bioavailability of inositol hexakisphosphates in terrestrial and aquatic environments. However, surface complexation and precipitation reactions of inositol hexakisphosphates on soil minerals have not been well understood. Here we investigate the surface complexation-precipitation process and mechanism of myo-inositol hexakisphosphate (IHP, phytate) on amorphous aluminum hydroxide (AAH) using macroscopic sorption experiments and multiple spectroscopic tools. The AAH (16.01 μmol m(-2)) exhibits much higher sorption density than boehmite (0.73 μmol m(-2)) and α-Al2O3 (1.13 μmol m(-2)). Kinetics of IHP sorption and accompanying OH(-) release, as well as zeta potential measurements, indicate that IHP is initially adsorbed on AAH through inner-sphere complexation via ligand exchange, followed by AAH dissolution and ternary complex formation; last, the ternary complexes rapidly transform to surface precipitates and bulk phase analogous to aluminum phytate (Al-IHP). The pH level, reaction time, and initial IHP loading evidently affect the interaction of IHP on AAH. In situ ATR-FTIR and solid-state NMR spectra further demonstrate that IHP sorbs on AAH and transforms to surface precipitates analogous to Al-IHP, consistent with the results of XRD analysis. This study indicates that active metal oxides such as AAH strongly mediate the speciation and behavior of IHP via rapid surface complexation-precipitation reactions, thus controlling the mobility and bioavailability of inositol phosphates in the environment.
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http://dx.doi.org/10.1021/es500996pDOI Listing
June 2014

The anthelmintic drug niclosamide induces apoptosis, impairs metastasis and reduces immunosuppressive cells in breast cancer model.

PLoS One 2014 8;9(1):e85887. Epub 2014 Jan 8.

State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu, China.

Breast carcinoma is the most common female cancer with considerable metastatic potential. Discovery of new therapeutic approaches for treatment of metastatic breast cancer is still needed. Here, we reported our finding with niclosamide, an FDA approved anthelmintic drug. The potency of niclosamide on breast cancer was assessed in vitro and in vivo. In this investigation, we found that niclosamide showed a dramatic growth inhibition against breast cancer cell lines and induced apoptosis of 4T1 cells in a dose-dependent manner. Further, Western blot analysis demonstrated the occurrence of its apoptosis was associated with activation of Cleaved caspases-3, down-regulation of Bcl-2, Mcl-1 and Survivin. Moreover, niclosamide blocked breast cancer cells migration and invasion, and the reduction of phosphorylated STAT3(Tyr705), phosphorylated FAK(Tyr925) and phosphorylated Src(Tyr416) were also observed. Furthermore, in our animal experiments, intraperitoneal administration of 20 mg/kg/d niclosamide suppressed 4T1 tumor growth without detectable toxicity. Histological and immunohistochemical analyses revealed a decrease in Ki67-positive cells, VEGF-positive cells and microvessel density (MVD) and an increase in Cleaved caspase-3-positive cells upon niclosamide. Notably, niclosamide reduced the number of myeloid-derived suppressor cells (MDSCs) in tumor tissues and blocked formation of pulmonary metastases. Taken together, these results demonstrated that niclosamide may be a promising candidate for breast cancer.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0085887PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3885752PMC
September 2014

High-spin states and level structure in stable nucleus strontium-84.

Sci Rep 2013 ;3:2740

1] Institute of Nuclear Energy Safety Technology, Chinese Academy of Sciences, Hefei 230031, China [2] School of Physics, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand [3] State Key Laboratory of Nuclear Physics and Technology (Peking University), Beijing 100871, China [4] Thailand Center of Excellence in Physics (ThEP), Commission on Higher Education, Thailand.

High-spin states of (84)Sr are populated through the reaction (70)Zn ((18)O, 4n) (84)Sr at the beam energy of 75 MeV. The measurements of excitation functions, γ-γ coincidences, directional correlations of oriented states (DCO) ratios and γ-transition intensities are performed using eight anticompton HPGe detectors and one planar HPGe detector. Based on the experimental results, we establish a new level scheme of (84)Sr, in which 12 new states and nearly 30 new γ-transitions are identified in the present work. The positive-parity yrast band is extended to spin I(π) = 24(+), while one negative-parity band is extended to spin I(π) = 19(-) and it is found that the even-spin and odd-spin members in high-spin states show the nature of signature staggering. The deformation of (84)Sr is studied by calculating the total-Routhian-surfaces (TRS) of positive-parity yrast states in the cranked shell model formalism.
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http://dx.doi.org/10.1038/srep02740DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3781391PMC
July 2014

Solid-state NMR spectroscopic study of phosphate sorption mechanisms on aluminum (Hydr)oxides.

Environ Sci Technol 2013 Aug 25;47(15):8308-15. Epub 2013 Jul 25.

Department of Geosciences and Center for Environmental Molecular Science, Stony Brook University , Stony Brook, New York 11794-2100, United States.

Sorption reactions occurring at mineral/water interfaces are of fundamental importance in controlling the sequestration and bioavailability of nutrients and pollutants in aqueous environments. To advance the understanding of sorption reactions, development of new methodology is required. In this study, we applied novel (31)P solid-state nuclear magnetic resonance (NMR) spectroscopy to investigate the mechanism of phosphate sorption on aluminum hydroxides under different environmental conditions, including pH (4-10), concentration (0.1-10 mM), ionic strength (0.001-0.5 M), and reaction time (15 min-22 days). Under these conditions, the NMR results suggest formation of bidentate binuclear inner-sphere surface complexes was the dominant mechanism. However, it was found that surface wetting caused a small difference. A small amount (<3%) of monodentate mononuclear inner-sphere surface complexes was observed in addition to the majority of bidentate binuclear surface complexes on a wet paste sample prepared at pH 5, which was analyzed in situ by a double-resonance NMR technique, namely, (31)P{(27)Al} rotational echo adiabatic passage double resonance (REAPDOR). Additionally, we found that adsorbents can substantially impact phosphate sorption not only on the macroscopic sorption capacity but also on their (31)P NMR spectra. Very similar NMR peaks were observed for phosphate sorbed to gibbsite and bayerite, whereas the spectra for phosphate adsorbed to boehmite, corundum, and γ-alumina were significantly different. All of these measurements reveal that NMR spectroscopy is a useful analytical tool for studying phosphorus chemistry at environmental interfaces.
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http://dx.doi.org/10.1021/es400874sDOI Listing
August 2013
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