Publications by authors named "Yunyun Li"

113 Publications

Correspondence between DOM molecules and microbial community in a subtropical coastal estuary on a spatiotemporal scale.

Environ Int 2021 Apr 23;154:106558. Epub 2021 Apr 23.

State Key Laboratory for Marine Environmental Science, Institute of Marine Microbes and Ecospheres, College of Ocean and Earth Sciences, Xiamen University, Xiang'an Campus, Xiang'an South Road, Xiamen 361102, China; Fujian Key Laboratory of Marine Carbon Sequestration, Xiamen University, Xiang'an Campus, Xiang'an South Road, Xiamen 361102, China. Electronic address:

Dissolved organic matter (DOM) changes in quantity and quality over time and space, especially in highly dynamic coastal estuaries. Bacterioplankton usually display seasonal and spatial variations in abundance and composition in the coastal regions, and influence the DOM pool via assimilation, transformation and release of organic molecules. The change in DOM can also affect the composition of bacterial community. However, little is known on the correspondence between DOM molecules and bacterial composition, particularly through a systematic field survey. In this study, the spatiotemporal signatures of microbial communities and DOM composition in the subtropical coastal estuary of Xiamen are investigated over one and half years. The co-occurrence analysis between bacteria and DOM suggested microorganisms likely transformed the DOM from a relatively high (>400 Da) to a low (<400 Da) molecular weight, corresponding to an apparent increase in overall aromaticity. This might be the reason why microbial transformation renders "dark" organic matter visible in mass spectrometry due to more efficient ionization of microbial metabolites, as well as photodegradation processes. K- and r-strategists exhibited different correlations with two-size categories of DOM molecules owing to their different lifestyles and responses to environmental nutrient conditions. A comparison of the environmental variables and DOM composition with the microbial communities showed that the environmental/DOM variations played a more important role in shaping the microbial communities than vice versa. This study sheds light on the interactions between microbial populations and DOM molecules at the spatiotemporal scale, improving our understanding of microbial roles in marine biogeochemical cycles.
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http://dx.doi.org/10.1016/j.envint.2021.106558DOI Listing
April 2021

Advection-enhanced diffusion in biased convection arrays.

Phys Rev E 2021 Mar;103(3):L030106

Department of Chemistry, Presidency University, Kolkata 700073, India.

We numerically investigated the transport of a passive colloidal particle in a one-dimensional periodic array of planar counter-rotating convection rolls at high Péclet numbers. We show that advection-enhanced diffusion is drastically suppressed by an external transverse bias but strongly reinforced by a longitudinal drive of appropriate intensity. Both effects are magnified by imposing free-slip flows at the array's edges. The dependence of the diffusion constant on an external forcing is interpreted as a measure of the fluid-mechanical robustness of the flow boundary layer mechanism governing diffusion in convection rolls.
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http://dx.doi.org/10.1103/PhysRevE.103.L030106DOI Listing
March 2021

Anisotropic Diffusion in Driven Convection Arrays.

Entropy (Basel) 2021 Mar 14;23(3). Epub 2021 Mar 14.

Department of Chemistry, Presidency University, Kolkata 700073, India.

We numerically investigate the transport of a Brownian colloidal particle in a square array of planar counter-rotating convection rolls at high Péclet numbers. We show that an external force produces huge excess peaks of the particle's diffusion constant with a height that depends on the force orientation and intensity. In sharp contrast, the particle's mobility is isotropic and force independent. We relate such a nonlinear response of the system to the advection properties of the laminar flow in the suspension fluid.
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http://dx.doi.org/10.3390/e23030343DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7999235PMC
March 2021

Linking the unique molecular complexity of dissolved organic matter to flood period in the Yangtze River mainstream.

Sci Total Environ 2021 Apr 8;764:142803. Epub 2020 Oct 8.

Key Laboratory of Geoscience Big Data and Deep Resource of Zhejiang Province, School of Earth Sciences, Zhejiang University, Hangzhou, China; State Key Laboratory of Satellite Ocean Environment Dynamics, Second Institute of Oceanography, Ministry of Natural Resources, Hangzhou, China. Electronic address:

Large rivers transport a significant amount of terrestrially derived dissolved organic matter (DOM) to coastal oceans, consisting of a critical component of the global biogeochemical cycle. Although high flow events usually introduce more terrestrial DOM than baseflow, the underlying molecular complexity and lability of DOM during high discharge are not well constrained, especially in large river ecosystems. By combining ultraviolet and fluorescent spectroscopy, and ultrahigh-resolution mass spectrometry, we found that stronger terrestrial DOM signal was detected during high discharge than normal discharge in the Yangtze River mainstream. The averaged DOC concentration was higher during high discharge than normal discharge. Optical properties confirmed higher aromaticity and relatively higher humic-like fluorescent components in DOM during high discharge. The molecular composition showed significantly higher molecular complexity, averaged molecular weight, aromaticity, relative abundances of polyphenols and highly unsaturated compounds of DOM during high discharge than normal discharge. A large set of unique molecular formulae (up to 4927) was only detected during high discharge. These unique molecular formulae were mostly lignin degradation products, likely due to more intensive soil leaching during high discharge. By comparing with incubation experiments and the Yangtze River mouth and East China Sea DOM molecular composition, some of these unique molecular formulae during high discharge are resistant to both bio- and photo-degradation, and persist during their transport to the East China Sea. Therefore, we suggest that high discharge will additionally introduce a relatively recalcitrant pool of DOM into the Yangtze River mainstream and persist during its journey to the ocean. Considering the projected increase of flood frequency, this study provides a preliminary foundation for further studies to better assess the underlying mechanisms how hydrology affect the biogeochemical cycling of DOM in large rivers.
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http://dx.doi.org/10.1016/j.scitotenv.2020.142803DOI Listing
April 2021

Hsa_circ_0015326 Promotes the Proliferation, Invasion and Migration of Ovarian Cancer Through miR-127-3p/MYB.

Cancer Manag Res 2021 10;13:2265-2277. Epub 2021 Mar 10.

Department of Gynaecology and Obstetrics, University-Town Hospital of Chongqing Medical University, Chongqing, People's Republic of China.

Background: More and more evidences show that circular RNA (circRNA) has an important role in ovarian cancer (OC). Hsa_circ_0015326 is a newly discovered upregulated circRNA in OC, but its role and mechanism in OC have not been studied yet.

Methods: Quantitative real-time PCR was used to detect the expression of hsa_circ_0015326, microRNA (miR)-127-3p and MYB. The viability, colony number, cell cycle process, invasion, migration and apoptosis of cells were determined using cell counting kit 8 assay, colony formation assay, flow cytometry, transwell assay and wound healing assay. Moreover, the protein expression levels of metastasis, proliferation, apoptosis markers and MYB were assessed using Western blot analysis. The interaction between miR-127-3p and hsa_circ_0015326 or MYB was confirmed by dual-luciferase reporter assay and RNA immunoprecipitation assay. Xenograft tumors were built to explore the role of hsa_circ_0015326 in OC tumor growth in vivo.

Results: Elevated expression of hsa_circ_0015326 was identified in OC tissues and cells. Loss-of-function experiments suggested that silenced hsa_circ_0015326 inhibited the proliferation, invasion, migration, and promoted the apoptosis of OC cells in vitro, as well as inhibited OC tumorigenesis in vivo. Mechanically, hsa_circ_0015326 sponged miR-127-3p and miR-127-3p targeted MYB. The rescue experiments revealed that miR-127-3p inhibitor reversed the inhibitory effect of hsa_circ_0015326 silencing on OC progression, and MYB overexpression reversed the suppressive effect of miR-127-3p on OC progression. In addition, our data indicated that MYB expression was positively regulated by hsa_circ_0015326.

Conclusion: This study showed that hsa_circ_0015326 could facilitate OC progression by regulating the miR-127-3p/MYB axis, which suggested that it might become a potential target for the treatment of OC.
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http://dx.doi.org/10.2147/CMAR.S291218DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7956866PMC
March 2021

A Xanthene Dye-based Sensor for Viscosity and Cell Imaging.

J Fluoresc 2021 May 20;31(3):719-725. Epub 2021 Feb 20.

Department of Environmental Engineering, School of Architecture and Civil Engineering, Chengdu University, Chengdu, 610106, Sichuan, China.

A new xanthene dye, namely ImX, has been facilely prepared by reaction of 4-(1H-Imidazol-1-yl)benzaldehyde with N, N-diethyl-3-aminophenol in concentrated propionic acid, and then treated by p-chloranil. ImX presents the maximum absorption and emission band centered at 562 nm and 583 nm in water, respectively. Fluorescent spectra investigations demonstrate that ImX shows viscosity-selective fluorescent response and emission enhancement when the solvent viscosity increases from 1.1 cp. (water) to 1248 cp. (98 % glycerol). In addition, this viscosity-selective fluorescence response covers a wide pH range from 2.5 to 10.0. More significantly, ImX demonstrates low cytotoxicity and can be employed as tracer for the detection of Monensin-triggered viscosity enhancement by cell imaging.
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http://dx.doi.org/10.1007/s10895-021-02705-4DOI Listing
May 2021

Plasma metabolomic profiling of patients recovered from COVID-19 with pulmonary sequelae 3 months after discharge.

Clin Infect Dis 2021 Feb 17. Epub 2021 Feb 17.

Department of Respiratory and Critical Care Medicine, NHC Key Laboratory of Pulmonary Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.

Background: Elucidation of the molecular mechanisms involved in the pathogenesis of coronavirus disease (COVID-19) may help to discover therapeutic targets.

Methods: To determine the metabolomic profile of circulating plasma from COVID-19 survivors with pulmonary sequelae 3 months after discharge, a random, outcome-stratified case-control sample was analyzed. We enrolled 103 recovered COVID-19 patients as well as 27 healthy donors, and performed pulmonary function tests, computerized tomography (CT) scans, laboratory examinations, and liquid chromatography-mass spectrometry.

Results: Plasma metabolite profiles of COVID-19 survivors with abnormal pulmonary function were evidently different from those of healthy donors or subjects with normal pulmonary function. These alterations were associated with disease severity and mainly involved amino acid, and glycerophospholipid metabolic pathways. Furthermore, increased levels of triacylglycerols, phosphatidylcholines, prostaglandin E2, arginine, and decreased levels of betain and adenosine were associated with pulmonary CO diffusing capacity and total lung capacity. The global plasma metabolomic profile differed between subjects with abnormal and normal pulmonary function.

Conclusions: Further metabolite-based analysis may help to identify the mechanisms underlying pulmonary dysfunction in COVID-19 survivors, and provide potential therapeutic targets in the future.
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http://dx.doi.org/10.1093/cid/ciab147DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7929060PMC
February 2021

Diffusion of active particles in convective flows.

Soft Matter 2021 Mar;17(8):2256-2264

Center for Phononics and Thermal Energy Science, Shanghai Key Laboratory of Special Artificial Microstructure Materials and Technology, School of Physics Science and Engineering, Tongji University, Shanghai 200092, China. and Dipartimento di Fisica, Università di Camerino, I-62032 Camerino, Italy.

We numerically investigated the diffusion of an active Janus particle in periodic arrays of planar counter-rotating convection rolls at high Péclet numbers. We considered convection patterns with distinct longitudinal and transverse advection properties and characterized the dependence of the relevant diffusion constants on the particle's dynamical parameters, namely, self-propulsion speed, correlation time and chirality. Numerical results are interpreted analytically based on qualitative arguments of classical transport theory. The purpose of the present analysis is controlling active matter transport in microfluidic devices.
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http://dx.doi.org/10.1039/d0sm01784aDOI Listing
March 2021

How permafrost degradation threatens boreal forest growth on its southern margin?

Sci Total Environ 2021 Mar 21;762:143154. Epub 2020 Oct 21.

College of Urban and Environmental Science, MOE Laboratory for Earth Surface Processes, Peking University, Beijing 100871, China.

Boreal forests are adapted to cold climates and are thus especially sensitive and vulnerable to climate change. In this study, we chose 10 plots covering different topographies at the southern edge of a boreal forest. The annual ring widths of the 249 Dahurican larch (Larix gmelinii) tree cores from these plots were measured and used to calculate the basal area increment (BAI) and the interannual sensitivity (Sx). We found that forests in 10 plots showed a significant change in Sx consistently around 1980. The growth of slope forest was significantly correlated with increases in temperature and precipitation, while the wetland forests, including bogs and peatland plateaus, responded negatively to temperature. In terms of precipitation, there was no effect in the peatland plateaus, but a negative effect occurred in bogs. Our results imply that the depth of the frost table could lead to different soil waterlogging from surplus water from thawing permafrost, resulting in different responses of tree growth to climate change.
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http://dx.doi.org/10.1016/j.scitotenv.2020.143154DOI Listing
March 2021

Corrigendum to "Spatiotemporal variability in urban HORECA food consumption and its ecological footprint in China" [Sci. Total Environ. 687 (2019) 1232-1244].

Sci Total Environ 2020 Dec 25;747:142343. Epub 2020 Sep 25.

Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, 11A, Datun Road, Chaoyang District, Beijing 100101, China.

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http://dx.doi.org/10.1016/j.scitotenv.2020.142343DOI Listing
December 2020

Hydrological management constraints on the chemistry of dissolved organic matter in the Three Gorges Reservoir.

Water Res 2020 Dec 19;187:116413. Epub 2020 Sep 19.

Organic Geochemistry Unit, School of Earth Sciences, Zhejiang University, Hangzhou 310027, China. Electronic address:

Reservoirs are well known as a far-reaching human modification on the functions of natural river networks. However, changes in the chemistry and reactivity of dissolved organic matter (DOM) responding to hydrological management for water retention structures, and its influence on the river carbon cycle, remain poorly understood. Here we show that hydrological management does shape the molecular composition of DOM in the world's largest Three Gorges Reservoir, as revealed by optical spectroscopy and ultrahigh-resolution mass spectrometry. Relatively higher terrestrial input, molecular complexity, isomeric complexity, and environmental stability of DOM were observed during the storage period, whereas the inverse occurred during the drainage period. The results demonstrate that the hydrodynamic processes, which are mainly controlled by water intrusion from mainstream to tributaries, are likely the underlying mechanism controlling DOM chemistry. Integrated with observations from worldwide river reservoirs, the DOM degradation experiments suggest that reservoir hydrological management would enhance DOM mineralization, thereby increase CO emission and change the river carbon cycle.
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http://dx.doi.org/10.1016/j.watres.2020.116413DOI Listing
December 2020

Source identification and component characterization of dissolved organic matter in an acid mine drainage reservoir.

Sci Total Environ 2020 Oct 1;739:139732. Epub 2020 Jun 1.

School of Resources and Environmental Engineering, Hefei University of Technology, Hefei, Anhui 230009, China; Key Laboratory of Nanominerals and Pollution Control of Anhui Higher Education Institutes, Hefei University of Technology, Hefei, Anhui 230009, China. Electronic address:

Acid mine drainage (AMD) is one of the most serious environmental problems and extreme environments on the earth, with high concentrations of sulphate and dissolved metals. A comprehensive description of dissolved organic matter (DOM) in these reservoirs is lacking, and it can play an important role in AMD pollution treatment and ecosystem. Thus, the source, composition and property of DOM in an AMD reservoir in Ma'an shan, China were studied using Fourier transform ion cyclotron resonance mass spectrometry and three-dimension excitation emission matrix fluorescence spectroscopy. The results suggested that the autochthonous algal metabolites significantly contributed to the DOM pool in the AMD reservoir. Bioavailable substances with lower oxidation, unsaturation and aromaticity such as lipids and carbohydrates were lacking in the AMD reservoir especially in the deeper layers. In addition, the proportion of sulfur compounds was significantly higher than that in other waters, suggesting the potential formation of organic matter with sulfur atom in a sulfur-rich environment. These findings underscore that the investigation of DOM in AMD reservoirs may offer references for the AMD treatment with addition of organic matter and broaden the understanding of special carbon cycling in the extreme environment of AMD.
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http://dx.doi.org/10.1016/j.scitotenv.2020.139732DOI Listing
October 2020

In-House Standard Method for Molecular Characterization of Dissolved Organic Matter by FT-ICR Mass Spectrometry.

ACS Omega 2020 May 14;5(20):11730-11736. Epub 2020 May 14.

State Key Laboratory of Heavy Oil Processing, Petroleum Molecular Engineering Center (PMEC), China University of Petroleum, Beijing 102249, China.

Electrospray ionization (ESI) coupled with Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) has been widely used for molecular characterization of dissolved organic matter (DOM). However, ESI FT-ICR MS generally has poor repeatability and reproducibility because of its inherent ionization mechanism and structural characteristics, which severely hindered its application in quantitative analysis of complex mixtures. In this article, we developed an in-house standard method for molecular characterization of DOM by ESI FT-ICR MS. Instead of obtaining reproducible results by determining the instrument parameters, we adopted an approach of object control on the mass spectrum to solve the problem of poor reproducibility. The mass peak shape, resolution, and relative intensity distribution of a natural organic matter standard were adjusted by optimizing the operating conditions to obtain a repeatable result. The quality control sample was run 26 times by the different operators in a 6-month-long period to evaluate the reproducibility. Results showed that the relative standard deviation (%) of repeatability and reproducibility are 1.02 and 2.35 for average H/C, respectively. The in-house standard method has been validated and successfully used for the characterization of more than 4000 DOM samples, which is transferable to other laboratories.
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http://dx.doi.org/10.1021/acsomega.0c01055DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7254807PMC
May 2020

Enhanced motility in a binary mixture of active nano/microswimmers.

Nanoscale 2020 May;12(17):9717-9726

Theoretical Quantum Physics Laboratory, RIKEN Cluster for Pioneering Research, Wako-shi, Saitama 351-0198, Japan and Department of Physics, University of Michigan, Ann Arbor, Michigan 48109-1040, USA.

It is often desirable to enhance the motility of active nano- or microscale swimmers such as, e.g., self-propelled Janus particles as agents of chemical reactions or weak sperm cells for better chances of successful fertilization. Here we tackle this problem based on the idea that motility can be transferred from a more active guest species to a less active host species. We performed numerical simulations of motility transfer in two typical cases, namely for interacting particles with a weak inertia effect, by analyzing their velocity distributions, and for interacting overdamped particles, by studying their effusion rate. In both cases, we detected motility transfer with a motility enhancement of the host species of up to a factor of four. This technique of motility enhancement can find applications in chemistry, biology and medicine.
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http://dx.doi.org/10.1039/d0nr01765eDOI Listing
May 2020

The application of the scallop nanostructure in deep silicon etching.

Nanotechnology 2020 Jul 14;31(31):315301. Epub 2020 Apr 14.

College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, People's Republic of China. Department of Semiconductor Etching, NAURA Technology Group Co., Ltd, Beijing 100176, People's Republic of China.

Micro/nanostructures with high aspect ratios in silicon wafers obtained by plasma etching are of great significance in device fabrication. In most cases, the scallop nanostructure in deep silicon etching should be suppressed. However, the scallop nanostructure could be applied in electronic device fabrication as characteristic information, which indicates the balance between deposition and etching. In this work, the applications of scallop nanostructures in etching process optimization and environmental protection are demonstrated. In addition, the minimum effect of the cycle time on the scallop size is reported for the first time. These results could bring new thoughts to the electronic devices related fields, such as micro-electro-mechanical systems (MEMS), silicon capacitors and advanced packaging.
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http://dx.doi.org/10.1088/1361-6528/ab88f0DOI Listing
July 2020

Assessment of Cd availability in rice cultivation (Oryza sativa): Effects of amendments and the spatiotemporal chemical changes in the rhizosphere and bulk soil.

Ecotoxicol Environ Saf 2020 Jun 7;196:110490. Epub 2020 Apr 7.

College of Resources and Environment, Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China. Electronic address:

Immobilization is widely used to decrease the availability of heavy metals, such as Cd and Pb, in contaminated soils. However, the spatial and temporal changes in the immobilization of soil by amendments combined with planting effects have not been studied well. In this study, unplanted and planted (with rice plants) pot experiments were used to assess the spatial and temporal changes in water-soluble Cd, Fe, Mn, and Ca. Soil properties, such as pH, redox potential (Eh), and dissolved organic carbon (DOC), were continuously recorded in both the rhizosphere and bulk soil using non-invasive rhizon samplers and a microelectrode system (Unisense). In unplanted soil, pH and Eh varied with time, but showed little radial variation from the rhizosphere to the bulk soil. The addition of hydrated lime (Ca(OH)) sharply increased the pH, DOC, and Ca content; decreased the Eh, Fe content, and Mn content; and gradually decreased the water-soluble Cd content in the soil profile. Hydroxyapatite showed no obvious effects in reducing Cd concentrations in different soil zones. The water-soluble Fe, Mn, Ca, and DOC content did not differ significantly between soil zones over time and a non-significant correlation with water-soluble Cd was shown. In planted soil, the pH increased while the Eh value decreased with an increase in the distance from the roots, regardless of the soil amendments used during the rice growth period. Hydroxyapatite gradually increased but hydrated lime decreased the water-soluble Cd in the rhizosphere. The concentration of water-soluble Cd in the rhizosphere was higher than that of the other soil zones during rice growth. These changes lead to more Cd uptake by roots and induced Cd accumulation in rice tissues. In addition, Cd and Fe concentration in iron plaque showed a significant positive correlation with Cd in rice, indicating that iron plaque promotes the uptake and accumulation of Cd in rice with soil amendments. Compared with the control, hydroxyapatite did not significantly affect the Cd content, while Ca(OH) significantly reduced the Cd content in iron plaque and rice tissues. In conclusion, the application of hydrated lime can significantly reduce the risk of Cd accumulation by rice in Cd-contaminated soils under flooding conditions.
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http://dx.doi.org/10.1016/j.ecoenv.2020.110490DOI Listing
June 2020

IQGAP2 Inhibits Migration and Invasion of Gastric Cancer Cells via Elevating SHIP2 Phosphatase Activity.

Int J Mol Sci 2020 Mar 13;21(6). Epub 2020 Mar 13.

Department of Immunology, School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China.

Previous studies have shown reduced expression of Src homology 2-containing inositol 5-phosphatase 2 (SHIP2) and its tumor-suppressive role in gastric cancer (GC). However, the precise role of SHIP2 in the migration and invasion of GC cells remains unclear. Here, an IQ motif containing the GTPase-activating protein 2 (IQGAP2) as a SHIP2 binding partner, was screened and identified by co-immunoprecipitation and mass spectrometry studies. While IQGAP2 ubiquitously expressed in GC cells, IQGAP2 and SHIP2 co-localized in the cytoplasm of GC cells, and this physical association was confirmed by the binding of IQGAP2 to PRD and SAM domains of SHIP2. The knockdown of either SHIP2 or IQGAP2 promoted cell migration and invasion by inhibiting SHIP2 phosphatase activity, activating Akt and subsequently increasing epithelial-mesenchymal transition (EMT). Furthermore, knockdown of IQGAP2 in SHIP2-overexpressing GC cells reversed the inhibition of cell migration and invasion by SHIP2 induction, which was associated with the suppression of elevated SHIP2 phosphatase activity. Moreover, the deletion of PRD and SAM domains of SHIP2 abrogated the interaction and restored cell migration and invasion. Collectively, these results indicate that IQGAP2 interacts with SHIP2, leading to the increment of SHIP2 phosphatase activity, and thereby inhibiting the migration and invasion of GC cells via the inactivation of Akt and reduction in EMT.
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http://dx.doi.org/10.3390/ijms21061968DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7139352PMC
March 2020

Effects of NIX-mediated mitophagy on ox-LDL-induced macrophage pyroptosis in atherosclerosis.

Cell Biol Int 2020 Jul 27;44(7):1481-1490. Epub 2020 Mar 27.

Department of General Surgery, Anhui Armed Police General Hospital, Hefei, 230032, Anhui, China.

Pyroptosis is a form of cell death that is uniquely dependent on caspase-1. Pyroptosis involved in oxidized low-density lipoprotein (ox-LDL)-induced human macrophage death through the promotion of caspase-1 activation is important for the formation of unstable plaques in atherosclerosis. The mitochondrial outer membrane protein NIX directly interacts with microtubule-associated protein 1 light chain 3 (LC3). Although we previously showed that NIX-mediated mitochondrial autophagy is involved in the clearance of damaged mitochondria, how NIX contributes to ox-LDL-induced macrophage pyroptosis remains unknown. Here, immunoperoxidase staining Nix expression decreased in human atherosclerosis. When we silenced NIX expression in murine macrophage cell, active caspase-1, and mature interleukin-1β expression levels were increased and LC3 was reduced. In addition, LDH release and acridine orange and ethidium bromide staining indicated that damage to macrophage cell membranes induced by ox-LDL was substantially worse. Moreover, intracellular reactive oxygen species and NLRP3 inflammasome levels increased. Taken together, these results demonstrated that NIX inhibits ox-LDL-induced macrophage pyroptosis via autophagy in atherosclerosis.
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http://dx.doi.org/10.1002/cbin.11343DOI Listing
July 2020

Organic fertilizer amendment increases methylmercury accumulation in rice plants.

Chemosphere 2020 Jun 11;249:126166. Epub 2020 Feb 11.

College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China. Electronic address:

Rice grains are a methylmercury (MeHg) intake route for humans, especially in certain mercury (Hg)-contaminated areas. For rice plant growth, animal manure is commonly used as an organic fertilizer; however, its role in the formation of MeHg in paddy soils remains poorly understood. The aims of this study were thus to explore 1) the effect of chicken manure (CH) and cow manure (CO) addition on the production of soil MeHg and the accumulation of MeHg in rice plants and 2) the mechanism by which CH and CO addition affect the bioaccumulation of MeHg in grains. A pot experiment with different levels of CH and CO was carried out with newly deposited Hg-contaminated paddy soil. Two microcosm experiments were performed to explore the associated mechanisms. The results of the pot experiment showed that 0.1-1% CH and CO addition promoted the biomass of rice plants by 10-23% and increased the soil MeHg concentration by 34-143%, which exhibited a significant positive correlation with brown rice MeHg content. Organic fertilizer addition significantly increased MeHg bioaccumulation in rice plants and the ratio of MeHg to total Hg (THg) in brown rice. Organic fertilizer also increased the abundance of microbial methylators. The results of the microcosm experiments showed that organic fertilizer addition enhanced dissolved THg concentrations in soil and consequently increased the soil MeHg concentration. These results suggested that applying organic fertilizer to newly deposited Hg-contaminated soil may increase MeHg accumulation in grains due to enhanced Hg release and microbial methylator activity, leading to environmental health concerns.
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http://dx.doi.org/10.1016/j.chemosphere.2020.126166DOI Listing
June 2020

Influence of calcium hydroxide addition on arsenic leaching and solidification/stabilisation behaviour of metallurgical-slag-based green mining fill.

J Hazard Mater 2020 05 22;390:122161. Epub 2020 Jan 22.

School of Civil and Resource Engineering, University of Science and Technology Beijing, Beijing, 100083, China.

In this study, metallurgical-slag-based binder (MSB) with different dosages of calcium hydroxide (CH) was mixed with high-arsenic-containing mine tailings (HAMT) to form green mining fill samples (GMFs) for As solidification/stabilisation (S/S). The As leaching characteristics of the GMFs were evaluated using pH-dependent leaching tests, semi-dynamic leaching tests and toxicity leaching tests. The effective diffusion coefficient (D) decreased from 6.98 × 10 to 5.90 × 10 cm/s and the leachability index (LI) increased from 13.53 to 14.73 after 3 wt.% CH was added to the GMFs. The GMFs containing 0 wt.% CH (GMF-0C) and those containing 3 wt.% CH (GMF-3C) reached pH = 2 with acid addition amounts of 9.0 meq/g-dry and 9.3 meq/g-dry at 90 d curing time, and the maximum As leaching concentrations of GMF-0C and GMF-3C reached 10.47 mg/L and 7.47 mg/L, respectively, indicating that GMF-3C exhibited better acid neutralisation and As retention capacities than GMF-0C. Further, a Tescan Integrated Mineral Analyser (TIMA) was used to analyse the dominant hydration products of GMF-3C, which revealed that calcium silicate hydrate, CASH, ettringite and zeolite phases represented approximately 22.5 wt.% of the products. These results provide an understanding regarding the safe large-scale utilisation of GMFs.
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http://dx.doi.org/10.1016/j.jhazmat.2020.122161DOI Listing
May 2020

SURGICAL OUTCOMES OF 25-GAUGE PARS PLANA VITRECTOMY USING AIR AS AN INTERNAL TAMPONADE FOR PRIMARY RHEGMATOGENOUS RETINAL DETACHMENT.

Retina 2020 Nov;40(11):2077-2082

Beijing Tongren Eye Center, Beijing Ophthalmology and Visual Sciences Key Laboratory, Beijing Tongren Hospital, Capital Medical University, Beijing, China; and.

Purpose: To report surgical outcomes of 25-gauge pars plana vitrectomy using air as an internal tamponade for patients with primary rhegmatogenous retinal detachment (RRD).

Methods: A retrospective clinical study of 59 eyes of 59 consecutive patients presented with primary RRD at the Beijing Tongren Eye Center in China. From August 2016 to May 2018, medical records of the patients who underwent 25-gauge pars plana vitrectomy with air tamponade for RRD were reviewed. The main outcome measures were primary and final anatomical success (retinal re-attachment) rates, and postoperative complications.

Results: Of the 59 patients, aged 54.47 ± 11.81 years, 31 (52.5%) were men. Vitrectomy was performed 3 to 40 (averaged 16.98 ± 10.17) days after the onset of symptoms, and the mean follow-up period was 12.90 ± 5.92 months (ranging 6.07-26.10 months). Forty-two eyes (71.2%) had RRD with retinal breaks in the superior half of the retina, and the mean number of retinal breaks was 1.75 ± 0.94. Three eyes (5.1%) had RRD with giant retinal tears. Of the 59 eyes, 35 (59.3%) had RRD with inferior quadrants involved. Proliferative vitreoretinopathy (PVR) gradings were C1 in 2 (3.4%) eyes and B or below in 57 (96.6%) eyes. The primary and final anatomical success rates were 94.9% (56/59) and 98.3% (58/59), respectively. Of the three eyes which developed re-detachment of the retina, one eye had postoperative progression of PVR and two eyes were RRD associated with macular hole in high myopia. Postoperative complications included 5 eyes (8.5%) with serous choroidal detachment within 3 days after surgery and 4 eyes (6.8%) with macular epiretinal membrane formation 1 to 8 months after surgery. Secondary cataract surgery was performed in 13 of the 53 phakic eyes (24.5%) during follow-up.

Conclusion: Small-gauge pars plana vitrectomy with air tamponade may be effective in treating selected cases of relatively simple primary RRD. Additional studies are needed to verify the efficacy of this surgical approach for more complicated cases such as those with giant retinal tears.
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http://dx.doi.org/10.1097/IAE.0000000000002744DOI Listing
November 2020

[Screening of interacting proteins of SHIP2 in human gastric mucosal epithelium by yeast two-hybrid system].

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2019 Nov;35(11):1000-1007

Department of Immunology, School of Basic Medicine, Anhui Medical University, Hefei 230032, China. *Corresponding author, E-mail:

Objective To screen a standard homogenous cDNA library of human gastric mucosal epithelium with yeast two-hybrid system and find the proteins that interact with Src-homology 2-containing inositol 5-phosphatase 2 (SHIP2). Methods Using the yeast two-hybrid system, P1 (SH2+5-Ptase) and P2 (PRD+SAM) segments of SHIP2 were used as bait proteins to screen the proteins that bind to SHIP2 from the homogenous cDNA library of human gastric mucosal epithelium. The selected interacting proteins of SHIP2 were verified by co-immunoprecipitation assay. Results A total of 39 positive clones were selected and sequenced for alignment analysis. It was verified that PHB interacted with SHIP2 by reductive hybridization and co-immunoprecipitation assays. Conclusion PHB, the interacting protein of SHIP2 was screened by yeast two-hybrid system from the homogenous cDNA library of human gastric mucosal epithelium.
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November 2019

Comparative study of the effects of different chelating ligands on the absorption and transport of mercury in maize (Zea mays L.).

Ecotoxicol Environ Saf 2020 Jan 6;188:109897. Epub 2019 Nov 6.

CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, 100049, China.

Mercury (Hg) pollution seriously threatens food safety and has attracted global attention. Phytoextraction, due to its low cost, applicability, and environmental friendliness, is considered a new technology for clean-up of heavy metal contamination in the environment. However, the low bioavailability of Hg in polluted areas greatly limits the applicability of phytoextraction. Here, we compared the effects of six common chelating ligands on the absorption and transport of Hg in maize (Zea mays L.), which has a high biomass and short growth cycle. The results showed that the root length and biomass of maize seedlings of the groups treated with the six chelating ligands (EDTA, iodide, ammonium, thiosulfate, thiocyanate, and thiocarbamide) did not change compared with those of the non-treated groups. Co-exposure to Hg and each chelating ligand markedly alleviated the inhibitory effect induced by Hg. Iodide treatment resulted in the lowest root Hg content and highest translocation factor (TF) value, while ammonium treatment gave rise to the highest shoot Hg concentration and lowest TF. Compared with other chelating ligands, thiosulfate exhibited the maximum alleviation of Hg toxicity and achieved the highest concentration of Hg in the roots and aerial parts. Moreover, the TF and Hg accumulation in the thiosulfate and Hg co-exposed group were much higher than those in the group exposed to Hg alone. This finding suggests that, among these common chelating ligands, thiosulfate compounds have great potential for Hg phytoextraction, while the others can immobilize Hg in polluted areas.
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http://dx.doi.org/10.1016/j.ecoenv.2019.109897DOI Listing
January 2020

Investigation into the semi-dynamic leaching characteristics of arsenic and antimony from solidified/stabilized tailings using metallurgical slag-based binders.

J Hazard Mater 2020 01 11;381:120992. Epub 2019 Aug 11.

School of Energy and Environment Engineering, University of Science and Technology Beijing, Beijing, 100083, China; Key Laboratory of High-efficient Mining and Safety of Metal Mines, Ministry of Education, Beijing, 100083, China; Key Laboratory of Resource-oriented Treatment of Industrial Pollutants, Beijing, 100083, China. Electronic address:

The leaching characteristics of metallurgical slag-based binders (MSB) solidified/stabilized tailings containing arsenic (As) and antimony (Sb), were investigated via a series of semi-dynamic leaching tests using three kinds of leachant, for the simulation of actual leaching conditions. The effectiveness of solidification/stabilization (S/S) treatment was evaluated by measuring the observed diffusion coefficients (D). It was found that MSB efficiently prevented As and Sb leaching, providing D values in the range of 10 to 10 cm/s and 10 to 10 cm/s, respectively, with the exception that the leaching mechanism of As was dissolution rather than diffusion under acetic acid leaching conditions. Physical encapsulation was found to be the dominant mechanism for Sb immobilization, while the dominant mechanism of As immobilization was precipitation in the monolithic MSB S/S treated tailings (MST). Results showed that the concentrations of leached As, Sb, Ca and Si, were affected by leachant pH and total acidity as well as the MSB constituent ratio. The effect of these parameters may be attributed to the stability of hydration products and their influence on the buffering capacity and structure of matrices, and the leachant pH and total acidity having the greatest influence on leaching characteristics.
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http://dx.doi.org/10.1016/j.jhazmat.2019.120992DOI Listing
January 2020

Spatiotemporal variability in urban HORECA food consumption and its ecological footprint in China.

Sci Total Environ 2019 Oct 11;687:1232-1244. Epub 2019 Jun 11.

Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Science, 11A, Datun Road, Chaoyang District, Beijing 100101, China.

Increasing hotel/restaurant/café (HORECA) food consumption (HFC) has become one of the most prominent features of food consumption transformation under the background of rapid urbanization in China. Combining direct-weighing data (using 11,883 dishes in 164 restaurants) and literature data, this study, for the first time, quantitatively calculated the amount of HFC and its ecological footprint (EF) in China in 2002 and 2015 to depict the environmental effects of this transformation. The results indicated that per capita HFC tripled from 320 g/cap/meal in 2002 to 852 g/cap/meal in 2015, representing an average annual growth rate of 13%. In addition, by scaling up the HFC in 31 provinces/municipalities/autonomous regions to the national scale, HFC in China increased by 12,612 tons from 2002 to 2015. Finally, the total EF in the HORECA sector increased nearly 33 times (or 44,440 global ha), from 1348 global ha in 2002 to 45,788 global ha in 2015. The EF of Guangdong increased the most, surpassing 4000 global ha in 2015. These findings provide a scientific basis to support improved food resource spatial allocation and mitigation of regional resource pressure to achieve sustainable consumption under the current background of rapid urbanization in China.
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http://dx.doi.org/10.1016/j.scitotenv.2019.06.148DOI Listing
October 2019

Toxoplasmic encephalitis of basal ganglia with tumor-like features proven by pathogen-specific polymerase chain reaction and direct DNA sequencing.

Neuropathology 2019 Oct 29;39(5):398-403. Epub 2019 Jul 29.

Department of Pathology, Changhai Hospital, the Second Military Medical University, Shanghai, China.

We report a case of a young female patient who developed progressive neurological dysfunction with a ring-enhancing tumor-like nodule on brain magnetic resonance imaging. Urgent surgery was performed to remove the mass in the left basal ganglia. Pathological findings showed that the necrotic brain areas were accompanied by congestion, edema, discrete hemorrhage, and intestinal and perivascular lymphohistiocytic infiltration. Immunohistochemical staining results showed that Toxoplasma gondii (T. gondii) immunoreactivity was detected in both cysts and tachyzoites in these areas. The glycerol-3-phosphate dehydrogenase gene (B1) of T. gondii was amplified by sequence-specific polymerase chain reaction (PCR) and the PCR products were bi-directional Sanger sequenced. A 195 bp consensus sequence of the gene B1 was found to be 98% identical to a reference T. gondii sequence (GenBank accession No. kx270373). The final diagnosis was toxoplasmic encephalitis in the left basal ganglia. This report suggests that PCR and bi-directional DNA sequencing of T. gondii gene might be the most convenient and rapid tools for accurate diagnosis of toxoplasmic encephalitis .
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http://dx.doi.org/10.1111/neup.12588DOI Listing
October 2019

Selenium decreases methylmercury and increases nutritional elements in rice growing in mercury-contaminated farmland.

Ecotoxicol Environ Saf 2019 Oct 17;182:109447. Epub 2019 Jul 17.

CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, And HKU-IHEP Joint Laboratory on Metallomics, And State Environmental Protection Engineering Center for Mercury Pollution Prevention and Control, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, 100049, China. Electronic address:

Methylmercury (MeHg) in rice grains grown in Hg-contaminated areas has raised environmental health concerns. Pot experiments found that selenium (Se) could reduce MeHg levels in rice grains. However, relatively high levels of Se (up to 6 mg/kg) were applied in these pot experiments, which may have adverse effects on the soil ecology due to the toxicity of Se. The aims of this work were thus to study 1) the effect of low levels of Se on the accumulation and distribution of Hg, especially MeHg, in rice plants grown in a real Hg-contaminated paddy field and 2) the effect of Se treatment on Se and other nutritional elements (e.g., Cu, Fe, Zn) in grains. A field study amended with different levels of Se was carried out in Hg-contaminated paddy soil in Qingzhen, Guizhou, China. The levels of MeHg and total Hg were studied using cold vapor atomic fluorescence spectrometry (CVAFS) and inductively coupled plasma mass spectrometry (ICP-MS). The distribution and relative quantification of elements in grains were examined by synchrotron radiation X-ray fluorescence analysis (SR-XRF). This field study showed that low levels of Se (0.5 μg/mL, corresponding to 0.15 mg Se/kg soils) could significantly reduce total Hg and MeHg in rice tissues. Se treatment also reduced Hg distribution in the embryo and endosperm and increased the levels of Fe, Cu, Zn and Se in grains and especially embryos. This field study implied that treatment with an appropriate level of Se is an effective approach to not only decrease the level of MeHg but to also increase the levels of nutritional elements such as Fe, Cu, Zn and Se in rice grains, which could bring beneficial effects for rice-dependent residents living in Hg-contaminated areas.
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http://dx.doi.org/10.1016/j.ecoenv.2019.109447DOI Listing
October 2019

Long non-coding RNA DQ786243 modulates the induction and function of CD4 Treg cells through Foxp3-miR-146a-NF-κB axis: Implications for alleviating oral lichen planus.

Int Immunopharmacol 2019 Oct 17;75:105761. Epub 2019 Jul 17.

Department of Stomatology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China.

The present study was aimed to investigate the effects of the long non-coding RNA DQ786243 in the regulation of Treg cells in oral lichen planus (OLP), as well as to evaluate its potential molecular mechanisms. Here we found that the expression of DQ786243 and Foxp3 were both overexpressed in the CD4 cells from the peripheral blood of OLP patients, and their expression was positively correlated. Meanwhile, compared with the normal CD4 cells, the frequency of Foxp3 Treg cells in the OLP CD4 cells was significantly higher. DQ786243 overexpression in normal CD4 cells resulted in the upregulation of Foxp3 and the higher frequency of Foxp3 Treg cells. Furthermore, we found that the induction of Foxp3 Treg cells by DQ786243 significantly increased its suppressive function, and suppressed the function of other CD4 T cells such as Th1 and Th17 by decreasing the levels of IFN-γ and IL-17. Moreover, we found that DQ786243 overexpression markedly elevated the expression of miR-146a via regulating Foxp3, and thus inhibiting the NF-κB signaling. In conclusion, these findings indicate that DQ786243 may regulate the induction and function of CD4 Treg cells through Foxp3-miR-146a-NF-κB axis, implicating a novel insight into understanding the progression of OLP.
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http://dx.doi.org/10.1016/j.intimp.2019.105761DOI Listing
October 2019

Elemental sulfur amendment enhance methylmercury accumulation in rice (Oryza sativa L.) grown in Hg mining polluted soil.

J Hazard Mater 2019 11 29;379:120701. Epub 2019 May 29.

CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, and HKU-IHEP Joint Laboratory on Metallomics, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

The influence of elemental sulfur (S(0)) amendment on methylmercury (MeHg) accumulation in rice and the chemical form of Hg in the rhizosphere were investigated under waterlogged conditions in Hg-contaminated soil (the majority of the Hg (˜70%) in forms similar to HgS). Different levels of S(0) addition increased the MeHg accumulation in rice. After a sequential extraction analysis of the chemical forms of Hg in the rhizosphere, the results showed that S(0) addition increased the organic bound Hg and decreased the residual Hg in the soils. An Hg L XANES further showed that S(0) addition increased the proportion of Hg in the form of RS-Hg-SR and decreased the proportion of Hg in the form of HgS, indicating that S(0) input may reactivate the non-bioavailable Hg in the rhizosphere and improve the net Hg methylation. These findings suggest that the application of S fertilizers to Hg-contaminated paddy soils may increase the MeHg concentration in the edible parts of crops, which may lead to more potential health problems in humans depending on the crop type. However, our study also suggests that S(0) addition could be an effective measure for mobilizing the insoluble Hg and accelerating the phytoremediation process in Hg-contaminated paddy soils.
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http://dx.doi.org/10.1016/j.jhazmat.2019.05.094DOI Listing
November 2019

Phonon-Assisted Electro-Optical Switches and Logic Gates Based on Semiconductor Nanostructures.

Adv Mater 2019 Aug 27;31(33):e1901263. Epub 2019 Jun 27.

Key Laboratory for Micro-Nano Physics and Technology of Hunan Province, College of Materials Science and Engineering, Hunan University, Changsha, 410082, China.

High-performance nanostructured electro-optical switches and logic gates are highly desirable as essential building blocks in integrated photonics. In contrast to silicon-based optoelectronic devices, with their inherent indirect optical bandgap, weak light-modulation mechanism, and sophisticated device configuration, direct-bandgap-semiconductor nanostructures with attractive electro-optical properties are promising candidates for the construction of nanoscale optical switches for on-chip photonic integrations. However, previously reported semiconductor-nanostructure optical switches suffer from serious drawbacks such as high drive voltage, limited operation spectral range, and low modulation depth. High-efficiency electro-optical switches based on single CdS nanobelts with low drive voltage, ultra-high on/off ratio, and broad operation wavelength range, properties resulting from unique electric-field-dependent phonon-assisted optical transitions, are demonstrated. Furthermore, functional NOT, NOR, and NAND optical logic gates are demonstrated based on these switches. These switches and optical logic gates represent an important step toward integrated photonic circuits.
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http://dx.doi.org/10.1002/adma.201901263DOI Listing
August 2019