Publications by authors named "Yunlong Wang"

161 Publications

Radiolytic Approach for Efficient, Selective and Catalyst-free CO2 Conversion at Room Temperature.

Chemphyschem 2021 Jul 2. Epub 2021 Jul 2.

Universite Paris-Saclay, Laboratory of Physical Chemistry, FRANCE.

The present study proposes a new approach for direct CO 2 conversion using primary radicals from water irradiation. In order to ensure reduction of CO 2 into CO 2 •- by all the hydrated electrons, we use formate ions to scavenge simultaneously the parent oxidizing radicals H • and OH • producing the same transient CO 2 •- radicals. Conditions are optimized to obtain the highest conversion yield of CO 2 . The goal is achieved under mild conditions of room temperature, neutral pH and 1 atm of CO 2 pressure. All the available radicals are exploited for selectively converting CO 2 into oxalate that is accompanied by H 2 evolution. Besides, at high CO 2 pressure, CO is also produced via presolvated electron scavenging and the H • radicals with CO 2 •- . The mechanism presented accounts for the results and also sheds light on the data in the literature. The radiolytic approach is a mild and scalable route of direct CO 2 capture at the source in industry and the products, oxalate salt and H 2 , can be easily separated.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/cphc.202100378DOI Listing
July 2021

Exploiting Causality for Improved Prediction of Patient Volumes by Gaussian Processes.

IEEE J Biomed Health Inform 2021 Jul 27;25(7):2487-2496. Epub 2021 Jul 27.

Estimating and surveillance volumes of patients are of great importance for public health and resource allocation. In many situations, the change of these volumes is correlated with many factors, e.g., seasonal environmental variables, medicine sales, and patient medical claims. It is often of interest to predict patient volumes and to that end, discovering causalities can improve the prediction accuracy. Correlations do not imply causations and they can be spurious, which in turn may entail deterioration of prediction performance if the prediction is based on them. By contrast, in this paper, we propose an approach for prediction based on causalities discovered by Gaussian processes. Our interest is in estimating volumes of patients that suffer from allergy and where the model and the results are highly interpretable. In selecting features, instead of only using correlation, we take causal information into account. Specifically, we adopt the Gaussian processes-based convergent cross mapping framework for causal discovery which is proven to be more reliable than the Granger causality when time series are coupled. Moreover, we introduce a novel method for selecting the history or look-back length of features from the perspective of a dynamical system in a principled manner. The quasi-periodicities that commonly exist in observations of volumes of patients and environment variables can readily be accommodated. Further, the proposed method performs well even in cases when the data are scarce. Also, the approach can be modified without much difficulty to forecast other types of patient volumes. We validate the method with synthetic and real-world datasets.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1109/JBHI.2021.3089459DOI Listing
July 2021

A High Precision Modeling Technology of Material Surface Microtopography and Its Influence on Interface Mechanical Properties.

Materials (Basel) 2021 May 28;14(11). Epub 2021 May 28.

School of Mechanical Engineering, Dalian University of Technology, Dalian 116024, China.

In order to accurately and effectively obtain the contact performance of the mating surface under the material surface topography characteristics, a numerical simulation method of rough surface based on the real topography characteristics and a multi-scale hierarchical algorithm of contact performance is studied in this paper. Firstly, the surface topography information of materials processed by different methods was obtained and characterized by a measuring equipment; Secondly, a non-Gaussian model considering kurtosis and skewness was established by Johnson transform based on Gaussian theory, and a rough surface digital simulation method based on real surface topography was formed; Thirdly, a multi-scale hierarchical algorithm is given to calculate the contact performance of different mating surfaces; Finally, taking the aeroengine rotor as the object, the non-Gaussian simulation method was used to simulate the mating surfaces with different topographies, and the multi-scale hierarchical algorithm was used to calculate the contact performance of different mating surfaces. Analysis results showed that the normal contact stiffness and elastic-plastic contact area between the mating surfaces of assembly 1 and assembly 2 are quite different, which further verifies the feasibility of the method. The contents of this paper allow to perform the fast and effective calculation of the mechanical properties of the mating surface, and provide a certain analysis basis for improving the surface microtopography characteristics of materials and the product performance.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ma14112914DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8198072PMC
May 2021

Effect on simultaneous removal of ammonia, nitrate, and phosphorus via advanced stacked assembly biological filter for rural domestic sewage treatment.

Biodegradation 2021 08 20;32(4):403-418. Epub 2021 Apr 20.

College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, China.

The discharge of ammonia-nitrogen (NH-N), total nitrogen (TN), chemical oxygen demand (COD), and total phosphorus (TP) in rural sewage usually exceeds the Pollutant Discharge Standard for Urban Sewage Treatment Plants (GB18918-2002). Efficient and cost-effective removal of these pollutants cannot be simultaneously realized using conventional rural sewage treatment methods. Thus, an assembled biological filter (D50 × W50 × H113 cm), including a phosphorus removal layer filled with solid polymeric ferric sulfate and alternating aerobic-anaerobic layers, is proposed herein. The aerobic (anerobic) layers were filled with zeolite (zeolite and composite soil) at different intervals. This system was used for the treatment of synthetic sewage having COD: 122.0-227.0 mg/L; NH-N: 29.1-47.0 mg/L; TN: 28.0-58.0 mg/L; and TP: 2.0-3.8 mg/L. Based on optimal operation conditions (40 L/h reflow rate, without artificial aeration, and 12-h operation cycle), the system showed NH-N, TN, COD, and TP removal efficiencies of 87.1  ±  8.1, 83.4  ±  7.9, 91.0  ±  9.4, and 80.0  ±  6.4%, respectively. Further, in the pilot-scale test, under the same optimal parameters, the removal efficiencies of NH-N, TN, COD, and TP were 78.9  ±  8.1, 75.4  ±  7.9, 82  ±  9.4, and 76  ±  6.4%, respectively. Furthermore, in the different functional units of the system, a large number of functional bacteria capable of efficiently facilitating the simultaneous removal of the different pollutants from sewage were identified. Therefore, this proposed system, which complies with current environmental discharge regulations, can be a more sustainable approach for the treatment of unattended rural sewage.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10532-021-09928-zDOI Listing
August 2021

TAD boundary and strength prediction by integrating sequence and epigenetic profile information.

Brief Bioinform 2021 Apr 19. Epub 2021 Apr 19.

College of Informatics, Huazhong Agricultural University, Wuhan, 430070, P. R. China.

Topologically associated domains (TADs) are one of the important higher order chromatin structures with various sizes in the eukaryotic genomes. TAD boundaries, as the flanking regions between adjacent domains, can restrict the interactions of regulatory elements, including enhancers and promoters, and are generally dynamic and variable in different cells. However, the influence of sequence and epigenetic profile-based features in the identification of TAD boundaries is largely unknown. In this work, we proposed a method called pTADS (prediction of TAD boundary and strength), to predict TAD boundaries and boundary strength across multiple cell lines with DNA sequence and epigenetic profile information. The performance was assessed in seven cell lines and three TAD calling methods. The results demonstrate that the TAD boundary can be well predicted by the selected shared features across multiple cell lines. Especially, the model can be transferable to predict the TAD boundary from one cell line to other cell lines. The boundary strength can be characterized by boundary score with good performance. The predicted TAD boundary and TAD boundary strength are further confirmed by three Hi-C contact matrix-based methods across multiple cell lines. The codes and datasets are available at https://github.com/chrom3DEpi/pTADS.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/bib/bbab139DOI Listing
April 2021

New Method and Electrophysiological Characteristics of LA Posterior Wall Isolation in Persistent Atrial Fibrillation.

Pacing Clin Electrophysiol 2021 Mar 18. Epub 2021 Mar 18.

Department of Cardiology, Beijing Anzhen Hospital, Capital Medical University, Beijing, China.

Background: Left atrial (LA) posterior wall isolation (PWI) is commonly used with persistent atrial fibrillation (AF) ablation. However, potentials are often still recorded in the posterior wall after pulmonary vein isolation (PVI), roof linear ablation, and bottom linear ablation in clinical practice. We aimed to explore the methodological approach and electrophysiological characteristics of PWI.

Methods: Thirty-six patients who attended our center with long-standing persistent AF were retrospectively analyzed. After routine PVI and roof and bottom linear ablation, complete PWI was confirmed in sinus rhythm by voltage mapping and high-output pacing. Otherwise, activation mapping and voltage mapping were used to guide ablation on the line or inside the posterior wall until bidirectional block was achieved.

Results: The first-pass success rate of PWI was 39%. In the remaining 61% of patients with posterior wall electrograms, activation mapping in sinus rhythm showed that the earliest activation point was not on the ablation line but in a relatively dispersed focal area, possibly related to epicardial muscular sleeve insertion. Voltage mapping revealed a focal high-voltage area in the posterior wall matching the relatively dispersed earliest activation site, in which an average of five points of ablation achieved complete PWI without serious esophageal injury. The middle zone contained 80% of the additional posterior wall ablation points.

Conclusions: PWI was performed safely and effectively with an average of five additional ablation points in the posterior wall in 61% of patients under the guidance of voltage mapping. This article is protected by copyright. All rights reserved.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/pace.14220DOI Listing
March 2021

Experimentally measuring the mode indices of Laguerre-Gaussian beams by weak measurement.

Opt Express 2021 Feb;29(4):5419-5426

As a special experimental technique, weak measurements extract very little information from the measured system and does not cause the measured state to collapse. When coupling the Laguerre-Gaussian (LG) state with a well-defined pre- and post-selected system of a weak measurement process, there will be an indirect interconnection between the expected value of coordinate operators of the final state and the mode indices of the measured LG state. The mode of the light is impacted very slightly after the weak measurement. Based on this we propose an experiment scheme and have managed to experimentally measure the mode indices of LG beams spanning from l = -6 to l = +6, p = 0 to p = +8 accurately with the final intensity distributions approximatly at their origin.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1364/OE.416671DOI Listing
February 2021

Leveraging graph-based hierarchical medical entity embedding for healthcare applications.

Sci Rep 2021 Mar 12;11(1):5858. Epub 2021 Mar 12.

Advanced Analytics, IQVIA Inc., Plymouth Meeting, PA, USA.

Automatic representation learning of key entities in electronic health record (EHR) data is a critical step for healthcare data mining that turns heterogeneous medical records into structured and actionable information. Here we propose ME2Vec, an algorithmic framework for learning continuous low-dimensional embedding vectors of the most common entities in EHR: medical services, doctors, and patients. ME2Vec features a hierarchical structure that encapsulates different node embedding schemes to cater for the unique characteristic of each medical entity. To embed medical services, we employ a biased-random-walk-based node embedding that leverages the irregular time intervals of medical services in EHR to embody their relative importance. To embed doctors and patients, we adhere to the principle "it's what you do that defines you" and derive their embeddings based on their interactions with other types of entities through graph neural network and proximity-preserving network embedding, respectively. Using real-world clinical data, we demonstrate the efficacy of ME2Vec over competitive baselines on diagnosis prediction, readmission prediction, as well as recommending doctors to patients based on their medical conditions. In addition, medical service embeddings pretrained using ME2Vec can substantially improve the performance of sequential models in predicting patients clinical outcomes. Overall, ME2Vec can serve as a general-purpose representation learning algorithm for EHR data and benefit various downstream tasks in terms of both performance and interpretability.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-021-85255-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7955058PMC
March 2021

Subunit E isoform 1 of vacuolar H+-ATPase OsVHA enables post-Golgi trafficking of rice seed storage proteins.

Plant Physiol 2021 Feb 24. Epub 2021 Feb 24.

State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China.

Dense vesicles (DVs) are Golgi-derived plant-specific carriers that mediate post-Golgi transport of seed storage proteins in angiosperms. How this process is regulated remains elusive. Here, we report a rice (Oryza sativa) mutant, named glutelin precursor accumulation8 (gpa8) that abnormally accumulates 57 kD proglutelins in the mature endosperm. Cytological analyses of the gpa8 mutant revealed that proglutelin-containing DVs were mistargeted to the apoplast forming electron-dense aggregates and paramural bodies (PMB) in developing endosperm cells. Differing from previously reported gpa mutants with post-Golgi trafficking defects, the gpa8 mutant showed bent Golgi bodies, defective trans-Golgi network (TGN), and enlarged DVs, suggesting a specific role of GPA8 in DV biogenesis. We demonstrated that GPA8 encodes a subunit E isoform 1 of vacuolar H+-ATPase (OsVHA-E1) that mainly localizes to TGN and the tonoplast. Further analysis revealed that the luminal pH of the TGN and vacuole is dramatically increased in the gpa8 mutant. Moreover, the colocalization of GPA1 and GPA3 with TGN marker protein in gpa8 protoplasts was obviously decreased. Our data indicated that OsVHA-E1 is involved in endomembrane luminal pH homeostasis as well as maintenance of Golgi morphology and TGN required for DV biogenesis and subsequent protein trafficking in rice endosperm cells.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/plphys/kiab099DOI Listing
February 2021

BRITTLE PLANT1 is required for normal cell wall composition and mechanical strength in rice.

J Integr Plant Biol 2021 May 22;63(5):865-877. Epub 2021 Mar 22.

State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, China.

A series of nucleotide sugar interconversion enzymes (NSEs) generate the activated sugar donors required for biosynthesis of cell wall matrix polysaccharides and glycoproteins. UDP-glucose 4-epimerases (UGEs) are NSEs that function in the interconversion of UDP-glucose (UDP-Glc) and UDP-galactose (UDP-Gal). The roles of UDP-glucose 4-epimerases in monocots remain unclear due to redundancy in the pathways. Here, we report a brittle plant (bp1) rice mutant that exhibits brittle leaves and culms at all growth stages. The mutant culms had reduced levels of rhamnogalacturonan I, homogalacturonan, and arabinogalactan proteins. Moreover, the mutant had altered contents of uronic acids, neutral noncellulosic monosaccharides, and cellulose. Map-based cloning demonstrated that OsBP1 encodes a UDP-glucose 4-epimerase (OsUGE2), a cytosolic protein. We also show that BP1 can form homo- and hetero-protein complexes with other UGE family members and with UDP-galactose transporters 2 (OsUGT2) and 3 (OsUGT3), which may facilitate the channeling of Gal to polysaccharides and proteoglycans. Our results demonstrate that BP1 participates in regulating the sugar composition and structure of rice cell walls.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/jipb.13050DOI Listing
May 2021

A Smartphone App to Support Sedentary Behavior Change by Visualizing Personal Mobility Patterns and Action Planning (SedVis): Development and Pilot Study.

JMIR Form Res 2021 Jan 27;5(1):e15369. Epub 2021 Jan 27.

Department of Computer and Information Science, University of Konstanz, Konstanz, Germany.

Background: Prolonged sedentary behavior is related to a number of risk factors for chronic diseases. Given the high prevalence of sedentary behavior in daily life, simple yet practical solutions for behavior change are needed to avoid detrimental health effects.

Objective: The mobile app SedVis was developed based on the health action process approach. The app provides personal mobility pattern visualization (for both physical activity and sedentary behavior) and action planning for sedentary behavior change. The primary aim of the study is to investigate the effect of mobility pattern visualization on users' action planning for changing their sedentary behavior. The secondary aim is to evaluate user engagement with the visualization and user experience of the app.

Methods: A 3-week user study was conducted with 16 participants who had the motivation to reduce their sedentary behavior. Participants were allocated to either an active control group (n=8) or an intervention group (n=8). In the 1-week baseline period, none of the participants had access to the functions in the app. In the following 2-week intervention period, only the intervention group was given access to the visualizations, whereas both groups were asked to make action plans every day and reduce their sedentary behavior. Participants' sedentary behavior was estimated based on the sensor data of their smartphones, and their action plans and interaction with the app were also recorded by the app. Participants' intention to change their sedentary behavior and user experience of the app were assessed using questionnaires.

Results: The data were analyzed using both traditional null hypothesis significance testing (NHST) and Bayesian statistics. The results suggested that the visualizations in SedVis had no effect on the participants' action planning according to both the NHST and Bayesian statistics. The intervention involving visualizations and action planning in SedVis had a positive effect on reducing participants' sedentary hours, with weak evidence according to Bayesian statistics (Bayes factor, BF=1.92; median 0.52; 95% CI 0.04-1.25), whereas no change in sedentary time was more likely in the active control condition (BF=0.28; median 0.18; 95% CI 0.01-0.64). Furthermore, Bayesian analysis weakly suggested that the more frequently the users checked the app, the more likely they were to reduce their sedentary behavior (BF=1.49; r=-0.50).

Conclusions: Using a smartphone app to collect data on users' mobility patterns and provide real-time feedback using visualizations may be a promising method to induce changes in sedentary behavior and may be more effective than action planning alone. Replications with larger samples are needed to confirm these findings.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2196/15369DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7875690PMC
January 2021

Preparation and preliminary evaluation of hepatitis B core antigen virus like nanoparticles loaded with indocyanine green.

Ann Transl Med 2020 Dec;8(24):1661

Henan Bioengineering Research Center, Zhengzhou, China.

Background: In recent years, nanotechnology has attracted a plethora of attention due of its ability to effectively diagnose and treat various tumors. Virus-like particles (VLPs) have good biocompatibility, are safe and non-toxic, and have an internal hollow space, and as such they are often used as nano drug carriers. In recent years, it has become one of the hot spots in the field of biopharmaceutical engineering.

Methods: In this study, the tumor-targeting peptide RGD (Arg-Gly-Asp) was genetically inserted into the major immunodominant region (MIR) of the hepatitis B virus core protein (HBc). A series of characterization, including stability and optical properties, were evaluated. A visual diagnosis and analysis of the efficacy against tumor cells were conducted at the cell level and using a live animal model.

Results: This study demonstrated that the recombinant HBc-based VLPs could participate in self-assembly of monodispersed nanoparticles with well-defined morphology, and the near-infrared dye indocyanine green (ICG) could be packaged into the VLPs without any chemical modification. Moreover, the HBc-based VLPs could specifically target cancer cells via the interaction with overexpressed integrin αvβ3. The treatment with ICG-loaded HBc-based VLPs showed significant inhibition of 4T1 breast cancer cell growth (84.87% tumor growth inhibition). The imaging experiments demonstrated that the ICG-loaded HBc-based VLPs generated excellent fluorescence in tumor sites in 4T1 breast cancer bearing mice. This provided crucial information on tumor mass location, boundaries, and shape. Moreover, compared to free ICG, the nanosystem showed significantly longer blood circulation time and superior accuracy in targeting the tumor.

Conclusions: The ICG-loaded HBc-based VLPs prepared in this study were of good stability and biocompatibility. It showed strong tumor targeting specificity and tumor visualization. Thus, it is expected to provide a new experimental basis and theoretical support for the integration of VLPs in the clinical diagnosis and treatment of breast cancer.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.21037/atm-20-7478DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7812214PMC
December 2020

Sustainable Chromium (VI) Removal from Contaminated Groundwater Using Nano-Magnetite-Modified Biochar via Rapid Microwave Synthesis.

Molecules 2020 Dec 28;26(1). Epub 2020 Dec 28.

Cranfield Water Science Institute, Cranfield University, College Road, Cranfield, Bedfordshire MK43 0AL, UK.

This study developed a nano-magnetite-modified biochar material (m-biochar) using a simple and rapid in situ synthesis method via microwave treatment, and systematically investigated the removal capability and mechanism of chromium (VI) by this m-biochar from contaminated groundwater. The m-biochar was fabricated from reed residues and magnetically modified by nano-FeO. The results from scanning electron microscopy (SEM) and X-ray diffraction (XRD) characterisations confirmed the successful doping of nano-FeO on the biochar with an improved porous structure. The synthesised m-biochar exhibited significantly higher maximum adsorption capacity of 9.92 mg/g compared with that (8.03 mg/g) of the pristine biochar. The adsorption kinetics followed the pseudo-second-order model and the intraparticle diffusion model, which indicated that the overall adsorption rate of Cr(VI) was governed by the processes of chemical adsorption, liquid film diffusion and intramolecular diffusion. The increasing of the pH from 3 to 11 significantly affected the Cr(VI) adsorption, where the capabilities decreased from 9.92 mg/g to 0.435 mg/g and 8.03 mg/g to 0.095 mg/g for the m-biochar and pristine biochar, respectively. Moreover, the adsorption mechanisms of Cr(VI) by m-biochar were evaluated and confirmed to include the pathways of electrostatic adsorption, reduction and complexation. This study highlighted an effective synthesis method to prepare a superior Cr(VI) adsorbent, which could contribute to the effective remediation of heavy metal contaminations in the groundwater.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/molecules26010103DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7795963PMC
December 2020

Biomimetic design of photonic materials for biomedical applications.

Acta Biomater 2021 02 7;121:143-179. Epub 2020 Dec 7.

Institute for Advanced Materials, School of Materials Science and Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu 212013, P. R. China. Electronic address:

Photonic crystal (PC) materials with bio-inspired structure colors have drawn increasing attention as their potentials have been rapidly progressed in the field of biomedicine. After elaborate integration with smart materials or preparations through advanced techniques, PC materials have shown significant advantages in biosensing, bio-probing, bio-screening, tissue engineering, and so forth. In this review, we first introduced the fundamentals of PC materials as well as their fabrication strategies with different dimensional outputs. Based on these diversified PC materials, their biomedical potentials as biosensing elements, cell carriers, drug delivery systems, screening methods, cell scaffolds for tissue engineering, cell imaging probes, as well as the monitoring means for biological processes were then highlighted. In addition to these, we finally listed and discussed some emerging applications of PCs integrated with functional materials and newly developed material engineering technologies. In short, this review will provide a panoramic view of PCs-based biomedicines, and moreover, the progressive discussions from fundamentals to advanced applications in this review may also encourage researchers to innovate PC materials or devices for broader biomedical applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.actbio.2020.12.008DOI Listing
February 2021

white panicle2 encoding thioredoxin z, regulates plastid RNA editing by interacting with multiple organellar RNA editing factors in rice.

New Phytol 2021 03 19;229(5):2693-2706. Epub 2020 Dec 19.

State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, China.

Thioredoxins (TRXs) occur in plant chloroplasts as complex disulphide oxidoreductases. Although many biological processes are regulated by thioredoxins, the regulatory mechanism of chloroplast TRXs are largely unknown. Here we report a rice white panicle2 mutant caused by a mutation in the thioredoxin z gene, an orthologue of AtTRX z in Arabidopsis. white panicle2 (wp2) seedlings exhibited a high-temperature-sensitive albinic phenotype. We found that plastid multiple organellar RNA editing factors (MORFs) were the regulatory targets of thioredoxin z. We showed that OsTRX z protein physically interacts with OsMORFs in a redox-dependent manner and that the redox state of a conserved cysteine in the MORF box is essential for MORF-MORF interactions. wp2 and OsTRX z knockout lines show reduced editing efficiencies in many plastidial-encoded genes especially under high-temperature conditions. An Arabidopsis trx z mutant also exhibited significantly reduced chloroplast RNA editing. Our combined results suggest that thioredoxin z regulates chloroplast RNA editing in plants by controlling the redox state of MORFs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/nph.17047DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8027827PMC
March 2021

Numerical Simulation and Accuracy Verification of Surface Morphology of Metal Materials Based on Fractal Theory.

Materials (Basel) 2020 Sep 18;13(18). Epub 2020 Sep 18.

School of Mechanical Engineering, Dalian University of Technology, Dalian 116024, China.

This paper presents a numerical simulation method to determine the surface morphology characteristics of metallic materials. First, a surface profiler (NV5000 5022s) was used to measure the surface, and the morphology data thereof were characterized. Second, fractal theory was used to simulate the surface profile for different fractal dimensions and scale coefficients , and statistical analyses of different surface morphologies were carried out. Finally, the fractal dimension of the simulated morphology and the actual morphology were compared. The analysis showed that the error of fractal dimension between the two morphologies was less than 10%; meanwhile, the comparison values of the characterization parameters of the simulated morphology and the actual morphology were approximately equal, and the errors were below 6%. Therefore, the current method used to evaluate the surface morphologies of parts processed by the grinding/milling method can be replaced by the simulated method using the corresponding parameters. This method makes it possible to theorize about the surface morphologies of machined parts, and provides a theoretical basis and reference value for the surface morphology design of materials, with the potential to improve the assembly quality of products.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ma13184158DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7560301PMC
September 2020

Effects of dietary vitamin D on growth performance, antioxidant capacities and innate immune responses in juvenile black carp Mylopharyngodon piceus.

Fish Physiol Biochem 2020 Dec 18;46(6):2243-2256. Epub 2020 Sep 18.

School of Life Science, Huzhou University, 759 East 2nd Road, Huzhou, 313000, People's Republic of China.

The aim of this experiment was used to investigate the effects of different contents of dietary vitamin D on the growth performance and antioxidant and innate immune responses in juvenile black carp Mylopharyngodon piceus. Black carp juveniles were fed six levels of dietary vitamin D (VD) (96, 220, 412, 840, 1480, and 3008 IU/Kg) for 9 weeks. Results showed that highest weight gain (WG) and special growth ratio (SGR) were obtained at 534.2 IU/Kg dietary VD according to the second-order polynomial regression model. The protein efficiency ratio (PER) of black carp could be significantly increased by 412, 840, and 1480 IU/Kg dietary VD (p < 0.05), while the feed conversion ratio (FCR) were reduced by 412, 840, and 1480 IU/Kg dietary VD (p < 0.05). Adequate dietary VD content (412, 840, and 1480 IU/Kg) could significantly upregulate expression levels of lipoxygenase 5 (LPO 5); increase the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), and glutathione reductase (GR); and improve GSH contents and total antioxidant capacities (T-AOC) in the liver of black carp. However, glutathione S-transferase (GST) activities and malondialdehyde (MDA) levels were significantly reduced by adequate dietary VD content (412, 840, and 1480 IU/Kg) in the fish liver. In addition, 412, 840, and 1480 IU/Kg dietary VD could significantly upregulate the mRNA expression levels of interferon-α (IFN-α), lysozyme (LYZ), hepcidin (HEPC), natural resistance-associated macrophage protein (NRAMP), and complement component 3 (C3) and C9 in the hemocytes and liver of black carp juveniles compared with the VD-deficient diet (96 IU/Kg). Meanwhile, higher contents of dietary VD could increase serum LYZ and ACP activities and C3 and C4 contents in black carp juveniles compared with the groups fed VD-deficient diet. In conclusion, these results suggest that adequate dietary VD could increase growth performances, improve antioxidant capacities, and then enhance innate immune parameters in black carp juveniles.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10695-020-00876-8DOI Listing
December 2020

Single-pixel foreground imaging without a priori background sensing.

Opt Express 2020 Aug;28(18):26018-26027

The single-pixel imaging technique, which is significantly different from conventional multi-pixel imaging, utilizes the signal recorded by a single-pixel detector and a stream of structured illumination patterns to reconstruct an image. We design and experimentally demonstrate a real-time single-pixel foreground imaging system with fewer samples and without a priori sensing of the background by performing incremental principal component analysis on online compressed sampling data. A fast ℓ compressed sensing algorithm is adopted to realize real-time foreground imaging of 10 frames per second with an image size of 127 × 127 pixels and a compression ratio of 3%. When applied to a surveillance system that requires long-distance video transmission, this scheme can greatly reduce the compression ratio and allow the system to work with smaller communication bandwidths.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1364/OE.400017DOI Listing
August 2020

Hypoxia exacerbates cardiomyocyte injury via upregulation of Wnt3a and inhibition of Sirt3.

Cytokine 2020 12 17;136:155237. Epub 2020 Aug 17.

Department of Cardiology, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China. Electronic address:

Ischemic injury is a major cause of several cardiovascular diseases, such as myocardial infarction, cardiac hypertrophy, and ventricular remodeling. Using an in vitro hypoxia model to mimic ischemia, we found that hypoxia stimulated Wnt3a expression. A mechanistic study showed that hypoxia-inducible factor 1α (HIF-1α) was directly recruited to the Wnt3a promoter. Wnt3a overexpression significantly decreased cell viability, promoted the generation of apoptotic cells, and enhanced hypoxia-induced injury in neonatal rat cardiomyocytes. This was partially through the upregulation of Caspase-3 mRNA levels and cleaved PARP-1 protein levels. In addition, we observed that Wnt3a exacerbated hypoxia-induced mitochondrial dysfunction and cytosolic release of cytochrome C. Furthermore, we found that Sirt3, a mitochondrial NAD-dependent deacetylase that modulates mitochondrial metabolism and homeostasis, was negatively regulated by Wnt3a. Conversely, Sirt3 overexpression repressed Wnt3a expression and ameliorated the hypoxia-induced mitochondrial dysfunction. Overall, our findings suggest that the hypoxia-Wnt3a-Sirt3 regulatory axis might be a potential target for cell protection in cardiac ischemia and hypoxia.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cyto.2020.155237DOI Listing
December 2020

OSlgg: An Online Prognostic Biomarker Analysis Tool for Low-Grade Glioma.

Front Oncol 2020 7;10:1097. Epub 2020 Jul 7.

Department of Predictive Medicine, Institute of Biomedical Informatics, Cell Signal Transduction Laboratory, Bioinformatics Center, Henan Provincial Engineering Center for Tumor Molecular Medicine, Kaifeng Key Laboratory of Cell Signal Transduction, School of Basic Medical Sciences, School of Software, Henan University, Kaifeng, China.

Glioma is the most frequent primary brain tumor that causes high mortality and morbidity with poor prognosis. There are four grades of gliomas, I to IV, among which grade II and III are low-grade glioma (LGG). Although less aggressive, LGG almost universally progresses to high-grade glioma and eventual causes death if lacking of intervention. Current LGG treatment mainly depends on surgical resection followed by radiotherapy and chemotherapy, but the survival rates of LGG patients are low. Therefore, it is necessary to use prognostic biomarkers to classify patients into subgroups with different risks and guide clinical managements. Using gene expression profiling and long-term follow-up data, we established an nline consensus urvival analysis tool for LGG named OSlgg. OSlgg is comprised of 720 LGG cases from two independent cohorts. To evaluate the prognostic potency of genes, OSlgg employs the Kaplan-Meier plot with hazard ratio and p value to assess the prognostic significance of genes of interest. The reliability of OSlgg was verified by analyzing 86 previously published prognostic biomarkers of LGG. Using OSlgg, we discovered two novel potential prognostic biomarkers ( and ) of LGG, and patients with the elevated expression of either or present the unfavorable survival outcome. These two genes may be novel risk predictors for LGG patients after further validation. OSlgg is public and free to the users at http://bioinfo.henu.edu.cn/LGG/LGGList.jsp.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fonc.2020.01097DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7381343PMC
July 2020

[Establishment of fluorescence immunochromatography detection for cytoskeleton-associated protein 4].

Sheng Wu Gong Cheng Xue Bao 2020 Jun;36(6):1216-1222

School of Life Sciences, Zhengzhou University, Zhengzhou 450000, Henan, China.

A rapid and simple method to detect tumor markers in liver cancer was established by combining immunochromatography technique with fluorescent microsphere labeling. According to the principle of double antibody sandwich, the cytoskeleton-associated protein 4 (CKAP4) paired antibody was used as the labeled and coated antibody, and the goat anti-rabbit polyclonal antibody was used as the quality control line coated antibody in the preparation of the CKAP4 fluorescent immunochromatographic test strips. After the preparation, the test strips were evaluated on various performance indicators, such as linearity, precision and stability. The CKAP4 immunochromatographic strip prepared by time-resolved fluorescent microspheres had high sensitivity, and good specificity. Its precision was within 15%, recovery between 85% and 115%, and linear range between 25 and 1 000 pg/mL. The test strip could be kept stable at 37 °C for 20 days, and it correlated well with commercial ELISA kits. The CKAP4 fluorescence immunochromatography method can quantitatively detect the content of CKAP4 in serum. Furthermore, it is rapid, sensitive, simple, economical and single-person operation. This method has the potential of becoming a new method for the diagnosis and treatment of liver cancer.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.13345/j.cjb.190412DOI Listing
June 2020

Quantitative Metabonomic Phenotypes in Different Structures of Mung Bean () Seeds and Their Germination-Associated Dynamic Changes.

J Proteome Res 2020 08 22;19(8):3352-3363. Epub 2020 Jun 22.

State Key Laboratory of Genetic Engineering, Zhongshan Hospital and School of Life Sciences, Human Phenome Institute, Metabonomics and Systems Biology Laboratory at Shanghai International Centre for Molecular Phenomics, Fudan University, Shanghai 200438, China.

Plant seed germination involving dynamic water uptakes and biochemical changes is essential for preservation of plant germplasm resource and worldwide food supply. To understand the germination-associated compartmental biochemistry changes, we quantitatively analyzed the metabolite composition (metabonome) for embryonic axes, cotyledons, and testae of mung bean () seeds in three germination phases using the NMR-based metabonomics approach. We found that three structures of mung bean seeds had distinct metabonomic phenotypes dominated by 53 metabolites including amino acids, carbohydrates, organic acids, choline metabolites, nucleotides/nucleosides, and shikimate-mediated secondary metabolites together with calcium and magnesium cations. During germination, all three seed structures had outstanding but distinct metabonomic changes. Both embryonic axis and cotyledon showed remarkable metabolic changes related to degradation of carbohydrates and proteins, metabolism of amino acids, nucleotides/nucleosides, and choline together with energy metabolism and shikimate-mediated plant secondary metabolism. The metabonomic changes in these two structures were mostly related to multiple functions for biochemical activities in the former and nutrient mobilizations in the latter. In contrast, testa metabonomic changes mainly reflected the metabolite leakages from the other two structures. Phase 1 of germination was featured with degradation of oligosaccharides and proteins and recycling of stored nucleic acids together with anaerobic metabolisms, whereas phase 2 was dominated by energy metabolism, biosynthesis of osmolytes, and plant secondary metabolites. These provided essential metabolic information for understanding the biochemistry associated with early events of seed germination and possible metabolic functions of different seed structures for plant development.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.jproteome.0c00236DOI Listing
August 2020

CD70-silenced dendritic cells induce immune tolerance in immune thrombocytopenia patients.

Br J Haematol 2020 11 18;191(3):466-475. Epub 2020 May 18.

State Key Laboratory of Experimental Hematology, National Clinical Research Center for Hematological Disorders, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, China.

The hyper-response of dendritic cell (DC) is believed to participate in the pathogenesis of immune thrombocytopenia (ITP). The CD70 expression on the surface of DCs that takes part in the CD27-CD70 costimulation pathway is an important element of DC 'licensing', which may initiate a series of autoreactive immune responses. To elucidate the roles CD70 molecules play in the DCs of ITP patients, we first stimulated the CD70 molecules on the DCs of ITP patients and normal controls, and found that the stimulated DCs from ITP patients exhibited higher ability to induce CD4 CD25 T lymphocytes proliferation, while lowering the ability of the induction of regulatory T cells (Tregs) from CD4 CD25 T lymphocytes. Meanwhile, higher IFN-γ and lower IL-10 levels were found in the co-culture system of stimulated DCs and CD4 CD25 T cells. We then silenced the CD70 genes on the induced DCs of ITP patients and normal controls by siRNA, and confirmed our suggestion that the silence of CD70 expression on the surface of DCs from ITP patients would decrease the CD4 CD25 T lymphocytes proliferation and Tregs differentiation, simultaneously inducing higher IL-10 and lower IFN-γ levels. Thus, the interference with the CD27-CD70 costimulatory pathway might lead to the alleviation of the consequent immune reactions, polarisation of Th2, induction of immune tolerance as well as shed new light on treatment of autoimmune diseases.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/bjh.16689DOI Listing
November 2020

Quantitative Metabonomic Analysis Reveals the Germination-Associated Dynamic and Systemic Biochemical Changes for Mung-Bean () Seeds.

J Proteome Res 2020 06 27;19(6):2457-2470. Epub 2020 May 27.

State Key Laboratory of Genetic Engineering, Zhongshan Hospital and School of Life Sciences, Human Phenome Institute, Metabonomics and Systems Biology Laboratory at Shanghai International Centre for Molecular Phenomics, Fudan University, Shanghai 200438, P. R. China.

Seed germination is essential for plant survival, germplasm resource preservation, and worldwide food supplies, although the germination-associated seed biochemical variations are not fully understood. With the NMR-based metabonomics, we quantitatively analyzed the comprehensive metabolite composition (metabonome) of mung-bean () seeds at eight time points of germination covering all three phases. We found that mung-bean seed metabonomes were dominated by 63 metabolites including lipids, amino acids, oligo-/monosaccharides, cyclitols, cholines, organic acids, nucleotides/-sides, nicotinates, and the shikimate pathway-mediated secondary metabolites. During germination, metabolic changes included mainly the degradation of proteins and raffinose family oligosaccharides, glycolysis, tricarboxylic acid (TCA) cycle, anaerobic respiration, biosynthesis of osmolytes and antioxidants together with the metabolisms of nucleotides/-sides, nicotinates, and amino acids. Oligosaccharide degradation was the primary energy source for germination, which coupled with the mobilization of starch and protein storages to produce sugars and amino acids for biomaterial and energy generations. Osmotic and redox regulations were prerequisites for seed germination together with mitochondrial reparations and generations to enable TCA cycle. During the postgermination growth stage (phase-3), the use of small molecules including amino acids and saccharides was switched to meet the growth demands of radicle cells. Small metabolites passed freely through seed testa leaking into the culture media during early germination but were reabsorbed by seed cells around the postgermination growth stage. Extra after-ripening accelerated these metabolic processes of seeds in phase-1, especially the biosynthesis of cyclitols, choline, and nicotinates, increasing the germination uniformity in terms of speed and percentage. Germination-resistant seeds were incapable of activating the germination-associated metabolic processes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.jproteome.0c00181DOI Listing
June 2020

Effect of Combined Pulmonary Vein and Superior Vena Cava Isolation on the Outcome of Second Catheter Ablation for Paroxysmal Atrial Fibrillation.

Am J Cardiol 2020 06 3;125(12):1845-1850. Epub 2020 Apr 3.

Department of Cardiology, Beijing Anzhen Hospital Affliated to Capital Medical University, Beijing, China. Electronic address:

Recurrence of paroxysmal atrial fibrillation (AF) is partly due to pulmonary vein (PV) reconnection and non-PV foci, especially superior vena cava (SVC). We aimed to investigate the efficacy and safety of empiric SVC isolation plus PV isolation after first failed radiofrequency ablation involving only PV isolation for paroxysmal AF. Procedural and follow-up outcomes of ablation for 144 consecutive paroxysmal AF patients after first failed radiofrequency ablation involving only PV isolation were retrospectively compared between patients undergoing either conventional SVC isolation (additional SVC isolation if SVC-triggered AF or rapid SVC activity was observed; n = 72) or empiric SVC isolation after PV isolation (n = 72). In conventional SVC isolation versus empiric SVC isolation groups: baseline characteristics and proportion of recorded PV electrical potentials were similarly distributed and all pulmonary veins were successfully reisolated; SVC isolation was performed less often (6 [8.3%] vs 70 [97.2%]; p <0.001, respectively); and during 19 ± 10 months follow-up, atrial tachyarrhythmias recurrence-free rate after a second procedure was lower (58.3% vs 77.8%, log rank; p = 0.037). Multivariate regression analysis revealed LA diameter ≥45 mm (odds ratio [OR] = 2.5; 95% confidence interval [CI], 1.4 to 4.6; p = 0.002) as the independent risk factor of atrial tachyarrhythmias recurrence and empiric SVC isolation (OR = 0.47; 95% CI, 0.25 to 0.87; p = 0.016) as the independent protector against atrial tachyarrhythmias recurrence after a second ablation procedure. Empiric SVC isolation plus PV isolation did not increase significantly procedural time or complications. In conclusion, the strategy of empiric SVC isolation plus PV isolation during a second procedure for paroxysmal AF improved atrial tachyarrhythmias recurrence-free rate without increasing procedural time or complications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.amjcard.2020.03.030DOI Listing
June 2020

OSeac: An Online Survival Analysis Tool for Esophageal Adenocarcinoma.

Front Oncol 2020 6;10:315. Epub 2020 Mar 6.

Cell Signal Transduction Laboratory, Bioinformatics Department of Predictive Medicine, Bioinformatics Center, Henan Provincial Engineering Center for Tumor Molecular Medicine, School of Software, School of Basic Medical Sciences, Institute of Biomedical Informatics, Henan University, Kaifeng, China.

Esophageal Adenocarcinoma (EAC) is one of the most common gastrointestinal tumors in the world. However, molecular prognostic systems are still lacking for EAC. Hence, we developed an nline consensus urvival analysis web server for sophageal denoarcinoma (OSeac), to centralize published gene expression data and clinical follow up data of EAC patients from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO). OSeac includes 198 EAC cases with gene expression profiling and relevant clinical long-term follow-up data, and employs the Kaplan Meier (KM) survival plot with hazard ratio (HR) and log rank test to estimate the prognostic potency of genes of interests for EAC patients. Moreover, we have determined the reliability of OSeac by using previously reported prognostic biomarkers such as , and . OSeac is free and publicly accessible at http://bioinfo.henu.edu.cn/EAC/EACList.jsp.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fonc.2020.00315DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7067743PMC
March 2020

Incidence of permanent pacemaker implantation after valve replacement surgery : Cardiac structure and function at 1-year follow-up.

Herz 2021 Apr 2;46(Suppl 1):109-114. Epub 2020 Mar 2.

Department of Cardiology, Beijing Anzhen Hospital, Beijing Institute of Heart, Lung& Blood Vessel Diseases, Capital Medical University, Beijing, China.

Background: Our study aimed to explore the incidence and risk factors of permanent pacemaker implantation (PPI) after valve replacement surgery (VR). The influence of long-term pacemaker dependency on cardiac structure and function at the 1‑year follow-up was also assessed.

Methods: The demographic and surgical data of all consecutive patients who underwent VR between 2013 and 2016 were collected. Univariate and multivariate analyses were performed to identify variables independently associated with PPI after VR. A 1‑year follow-up was undertaken of patients who underwent dual-chambers pacemaker after VR because of complete atrioventricular block (AVB). Long-term pacemaker dependency and recovery of cardiac structure and function were evaluated.

Results: There were 5320 consecutive patients with VR. The incidence of postoperative PPI was 2.42%. Multivariate analysis indicated that among the 62 patients who underwent PPI due to AVB and sick sinus syndrome, isolated aortic valve replacement (AVR; OR: 2.24, p < 0.05), VR combined with ventricular septal defect (VSD) repair (OR: 6.78, p < 0.05), and VR with aortic root and arch surgery (OR: 4.14, p < 0.05) were independent predictors of PPI after surgery. In total, 89.6% (43/48) of the survivors showed pacemaker dependency. Of these 43 patients, 24 had enlarged left heart before VR. Compared with preoperative values, the left atrial and left ventricular end-diastolic diameter post-PPI decreased significantly, while left ventricular ejection fraction was not significantly different.

Conclusion: Isolated AVR, VR concomitant with VSD repair, and VR with aortic root and arch surgery are independent predictors of PPI after VR. The majority of patients do not recover from AVB disorders and there is no significant negative effect on recovery of cardiac structure and function.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00059-020-04895-2DOI Listing
April 2021

Systematic Comparison of Two Animal-to-Human Transmitted Human Coronaviruses: SARS-CoV-2 and SARS-CoV.

Viruses 2020 02 22;12(2). Epub 2020 Feb 22.

Department of Preventive Medicine, Institute of Biomedical Informatics, Bioinformatics Center, Henan Provincial Engineering Center for Tumor Molecular Medicine, School of Basic Medical Sciences, Henan University, Kaifeng 475004, China.

After the outbreak of the severe acute respiratory syndrome (SARS) in the world in 2003, human coronaviruses (HCoVs) have been reported as pathogens that cause severe symptoms in respiratory tract infections. Recently, a new emerged HCoV isolated from the respiratory epithelium of unexplained pneumonia patients in the Wuhan seafood market caused a major disease outbreak and has been named the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). This virus causes acute lung symptoms, leading to a condition that has been named as "coronavirus disease 2019" (COVID-19). The emergence of SARS-CoV-2 and of SARS-CoV caused widespread fear and concern and has threatened global health security. There are some similarities and differences in the epidemiology and clinical features between these two viruses and diseases that are caused by these viruses. The goal of this work is to systematically review and compare between SARS-CoV and SARS-CoV-2 in the context of their virus incubation, originations, diagnosis and treatment methods, genomic and proteomic sequences, and pathogenic mechanisms.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/v12020244DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7077191PMC
February 2020

Manufacture and characteristics of HA-Electrodeposited polylactic acid/polyvinyl alcohol biodegradable braided scaffolds.

J Mech Behav Biomed Mater 2020 03 27;103:103555. Epub 2019 Nov 27.

Innovation Platform of Intelligent and Energy-Saving Textiles, School of Textile Science and Engineering, Tiangong University, Tianjin, 300387, China; Tianjin and Ministry of Education Key Laboratory for Advanced Textile Composite Materials, Tiangong University, Tianjin, 300387, China; Fujian Key Laboratory of Novel Functional Fibers and Materials, Minjiang University, Fuzhou, 350108, China; College of Textile and Clothing, Qingdao University, Shandong, 266071, China; Department of Bioinformatics and Medical Engineering, Asia University, Taichung, 41354, Taiwan; Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, 40402, Taiwan. Electronic address:

This study proposes the braided bone scaffolds. First, biologically degradable polylactic acid (PLA) filaments and polyvinyl alcohol (PVA) filaments are plied into composite yarns using a doubling and twisting machine. The composite yarns are tested to determine the optimal mechanical properties and a stabilized morphology. The PLA/PVA composite yarns are then braided into bone scaffolds, during which the optimal braiding process parameters and yarn ratio are determined. Based on the surface observation and tensile strength, a gear ratio of 45:45 provides the tubular braids with an optimal morphology and porosity that meet the biological requirements. When the PLA/PVA ratio is 3:1, the braids exhibit the maximum tensile properties and the most stable space structure. Furthermore, to make the braids a bioactive material with surface active sites, the braids are coated with hydroxyapatite (HA) by electrodeposition. The resulting HA-electrodeposited bone scaffolds are tested by in vitro biological experiments using a scanning electronic microscope (SEM), energy dispersive x-ray analysis(EDAX), X-ray Diffraction(XRD), and Fourier transform infrared spectroscopy(FT-IR), thereby examining their characteristics and microstructure. Results suggest that HA is electrodeposited over the bone scaffolds successfully. The immersion in simulated body fluid (SBF) is proven to contribute a good in vitro bioactivity to bone scaffolds. As a result, bone scaffolds are a good candidate for the application in the cancellous bone repairing field.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jmbbm.2019.103555DOI Listing
March 2020

Irisin promotes cementoblast differentiation via p38 MAPK pathway.

Oral Dis 2020 Jul 4;26(5):974-982. Epub 2020 Mar 4.

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, China.

Objective: Irisin is a newly identified exercise-induced myokine which can affect glucose metabolism and cortical bone mass and strength. However, the influence of irisin on cementoblasts remains largely unknown.

Material And Methods: An immortalized mouse cementoblast cell line OCCM-30 was used in this study. Cementoblast differentiation markers and PGC-1α in cells cultured with mineral induction medium were evaluated by qRT-PCR. Cementoblast mineralization was evaluated by alizarin red staining. Differentiation markers and the activity of p38 MAPK pathway under irisin stimulation were assessed by qRT-PCR or Western blot analysis. p38 MAPK pathway inhibitor SB203580 or p38 siRNA was used to further identify the regulatory mechanism. Cell proliferation treated with irisin was examined by CCK-8 method.

Results: The expression of Runx2, osterix, ALP, and PGC-1α was up-regulated consistently under mineral induction. The formation of mineralized nodules was increased by irisin. Runx2, osterix, ALP, and osteocalcin were obviously up-regulated under irisin stimulation as well as the activity of p38 MAPK pathway. When pretreated with SB203580 or p38 siRNA before irisin stimulation, the irisin-induced differentiation was distinctly suppressed. OCCM-30 cell proliferation was enhanced when treated with high-dose irisin for long time.

Conclusion: Irisin can promote the differentiation of cementoblasts via p38 MAPK pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/odi.13307DOI Listing
July 2020
-->