Publications by authors named "Yunhui Li"

117 Publications

The Release of Indium Ion Derived from Epithelial Cells and Macrophages Solubilization Contribute to Pneumotoxicity Induced by Indium Oxide Nanoparticles.

J Nanosci Nanotechnol 2021 12;21(12):6007-6015

Key Laboratory of Environmental Medicine Engineering of Ministry of Education, School of Public Health, Southeast University, Nanjing, Jiangsu 210000, China.

Occupational exposure to indium oxide and indium containing particles has been associated with the development of severe lung diseases called "indium lung." According to the survey of occupational hygiene, indium oxide nanoparticles have been identified in the workplaces and the lungs of workers. To date, the potential mechanism of the pneumotoxicity has been poorly understood and no effective therapies are available against "indium lung." Our present study reported that the exposure of indium oxide nanoparticles damaged lung epithelial cells and alveolar macrophages and induced pulmonary alveolar proteinosis and inflammation in rats. In the 8-week post-exposure period, the indium oxide nanoparticles still mostly accumulated in the lungs and then persistently release indium ions in two months after exposure. , the epithelial cells show the greater potential for release of indium ions from indium oxide nanoparticles compared with the macrophages. EDTA-2Na, a metal chelating agent expected to remove the indium ions, was found to significantly reduced the cytotoxicity of indium oxide nanoparticles. Herein, the pneumotoxicity may be attributed to the slow and incremental release of indium ions from indium oxide nanoparticles primary dissolved by epithelial cells and macrophages, at least partially. The study may provide some insights to the pathogenicity mechanisms of "indium lung" and some clues against the health hazards of occupational inhaled indium oxide nanoparticles at the workplaces.
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http://dx.doi.org/10.1166/jnn.2021.19498DOI Listing
December 2021

SARS-CoV-2 Spike protein enhances ACE2 expression via facilitating Interferon effects in bronchial epithelium.

Immunol Lett 2021 Jul 3;237:33-41. Epub 2021 Jul 3.

National Key Laboratory of Medical Immunology & Institute of Immunology, Second Military Medical University, Shanghai 200433, China. Electronic address:

Objective: In this study, we focused on the interaction between SARS-CoV-2 and host Type I Interferon (IFN) response, so as to identify whether IFN effects could be influenced by the products of SARS-CoV-2.

Methods: All the structural and non-structural proteins of SARS-CoV-2 were transfected and overexpressed in the bronchial epithelial cell line BEAS-2B respectively, and typical antiviral IFN-stimulated gene (ISG) ISG15 expression was detected by qRT-PCR. RNA-seq based transcriptome analysis was performed between control and Spike (S) protein-overexpressed BEAS-2B cells. The expression of ACE2 and IFN effector JAK-STAT signaling activation were detected in control and S protein-overexpressed BEAS-2B cells by qRT-PCR or/and Western blot respectively. The interaction between S protein with STAT1 and STAT2, and the association between JAK1 with downstream STAT1 and STAT2 were measured in BEAS-2B cells by co-immunoprecipitation (co-IP).

Results: S protein could activate IFN effects and downstream ISGs expression. By transcriptome analysis, overexpression of S protein induced a set of genes expression, including series of ISGs and the SARS-CoV-2 receptor ACE2. Mechanistically, S protein enhanced the association between the upstream JAK1 and downstream STAT1 and STAT2, so as to promote STAT1 and STAT2 phosphorylation and ACE2 expression.

Conclusion: SARS-CoV-2 S protein enhances ACE2 expression via facilitating IFN effects, which may help its infection.
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http://dx.doi.org/10.1016/j.imlet.2021.06.008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8254647PMC
July 2021

Analysis of perinatal coagulation function in preeclampsia.

Medicine (Baltimore) 2021 Jul;100(26):e26482

Shengjing Hospital of China Medical University, Shenyang, Liaoning, China.

Abstract: To study the dynamic changes in perinatal coagulation function in patients with preeclampsia (PE).The general data and coagulation indexes of 290 PE patients during the perinatal period (prenatal and 1 and 3 days postpartum) and 256 healthy pregnant women in the third trimester of pregnancy were investigated, and the data were analyzed.Compared with healthy pregnant women, prothrombin time (PT), fibrinogen (FIB), platelet count (PLT), mean platelet volume (MPV), thrombocytocrit (PCT), maximum amplitude (MA), and coagulation index (CI) of PE patients decreased, and activated partial thrombin time (APTT), thrombin time (TT), D-dimer (DD), platelet distribution width (PDW) and K values increased before delivery (P < .05). APTT and FIB in PE patients were lower in the day 1 postpartum group than in the prenatal and postpartum day 3 groups, and TT, DD, and fibrin degradation products (FDP) were higher (P < .05). PCT and MPV were highest in the prenatal group (P < .05).Compared with that of healthy pregnant women, the coagulation function of PE patients is in a relatively low-coagulation and high-fibrinolysis state on postpartum day 1, which increases the risk of postpartum hemorrhage and other adverse outcomes.
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http://dx.doi.org/10.1097/MD.0000000000026482DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8257867PMC
July 2021

Reproductive toxicity and underlying mechanisms of di(2-ethylhexyl) phthalate in nematode Caenorhabditis elegans.

J Environ Sci (China) 2021 Jul 6;105:1-10. Epub 2021 Jan 6.

Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China. Electronic address:

DEHP (di(2-ethylhexyl) phthalate) is an endocrine disruptor commonly found in plastic products that has been associated with reproduction alterations, but the effect of DEHP on toxicity is still widely unknown. Using DEHP concentrations of 10, 1, and 0.1 mg/L, we showed that DEHP reduced the reproductive capacity of Caenorhabditis elegans after 72 hr. of exposure. DEHP exposure reduced the reproductive capacity in terms of decreased brood sizes, egg hatchability (0.1, 1 and 10 mg/L), and egg-laying rate (1 and 10 mg/L), and increased numbers of fertilized eggs in the uterus (1 and 10 mg/L). DEHP also caused damage to gonad development. DEHP decreased the total number of germline cells, and decreased the relative area of the gonad arm of all exposure groups, with worms in the 1 mg/L DEHP exposure group having the minimum gonad arm area. Additionally, DEHP caused a significant concentration-dependent increase in the expression of unc-86. Autophagy and ROS contributed to the enhancement of DEHP toxicity in reducing reproductive capacity, and glutathione peroxidase and superoxide dismutase were activated as the antioxidant defense in this study. Hence, we found that DEHP has a dual effect on nematodes. Higher concentration (10 mg/L) DEHP can inhibit the expression of autophagy genes (atg-18, atg-7, bec-1, lgg-1 and unc-51), and lower concentrations (0.1 and 1 mg/L) can promote the expression of autophagy genes. Our data highlight the potential environmental risk of DEHP in inducing reproductive toxicity toward the gonad development and reproductive capacity of environmental organisms.
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http://dx.doi.org/10.1016/j.jes.2020.12.016DOI Listing
July 2021

Anti-N-Methyl-D-Aspartate Receptor Encephalitis Associated with Ovarian Teratoma in South China-Clinical Features, Treatment, Immunopathology, and Surgical Outcomes of 21 Cases.

Dis Markers 2021 21;2021:9990382. Epub 2021 May 21.

Department of Gynecology, Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China.

Objective: To study the clinical characteristics and surgical outcomes of anti-NMDAR encephalitis and the immunopathology of associated teratomas.

Methods: Twenty-one patients were enrolled in this retrospective study, who were diagnosed with anti-NMDAR encephalitis with ovarian teratoma and admitted to two tertiary hospitals in South China from July 2014 to December 2019. The clinical data of patients were reviewed. Comparisons were made between the patients with different outcomes after surgery. Immunohistochemical analyses of associated ovarian teratomas were performed.

Results: The mean age of the patients was 24.33 ± 5.12 years. The peak seasons of disease onset were autumn and winter (30.61% and 32.65%). The symptoms could be divided into 8 categories, including psychiatric abnormalities, seizures, movement dysfunction, consciousness disorders, autonomic dysregulation, speech disturbance, central hypoventilation, and memory deficits. All patients developed four or more categories of symptoms within the first four weeks. Twelve patients (57.1%) had a maximum mRS of 5, and 11 patients (52.4%) were admitted to ICU. Twenty patients received surgery, and only 3 patients were diagnosed pathologically with immature ovarian teratomas, while the other 17 patients had mature ovarian teratomas. After surgery, 17 patients (85.0%) got clinical improvement. The central hypoventilation symptom and mature ovarian teratomas were associated with surgical outcome. Immunohistochemical analysis revealed that there were NMDAR-positive neural tissues in all 8 teratomas and in which 3 cases also contained large numbers of NMDAR-positive sebaceous glands and squamous epithelial tissues.

Conclusion: The disease is of high prevalence in autumn and winter. The central hypoventilation symptom and mature ovarian teratomas were associated with surgical outcome. NMDAR-positive neural tissue is not the only etiological factor of encephalitis. We speculate that encephalitis development in some patients may result from NMDAR expression in sebaceous glands and squamous epithelial tissues.
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http://dx.doi.org/10.1155/2021/9990382DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8163540PMC
May 2021

Calcium Channel Subunit α2δ-1 as a Potential Biomarker Reflecting Illness Severity and Neuroinflammation in Patients with Acute Ischemic Stroke.

J Stroke Cerebrovasc Dis 2021 Aug 25;30(8):105874. Epub 2021 May 25.

Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan 430071, Hubei, China. Electronic address:

Background: Voltage-gated calcium channels (VGCCs) dysfunction is involved in the development of acute ischemic stroke (AIS). As a subunit of VGCC complexes, we detected the levels of α2δ-1 subunit in serum and cerebrospinal fluid (CSF) specimens from AIS patients.

Methods: The study included 105 patients with first-ever AIS, who were admitted within 48 hours after stroke onset. The serum and CSF levels of α2δ-1 were measured with ELISA and the severity of AIS patients was evaluated according to the National Institutes of Health Stroke Scale (NIHSS) score. The cerebral infarct volume was calculated through the Pullicino formula based on the cranial CT or MRI scan. C-reactive protein (CRP) and serum amyloid A (SAA) were measured using the latex-enhanced immunoturbidimetric assay.

Results: Compared to the control subjects, the serum α2δ-1 level was significantly increased in AIS patients with large infarct volume and in severe AIS cases with high NIHSS score, which correlated positively with the inflammatory markers CRP and SAA. Furthermore, the concentration of α2δ-1 in CSF was elevated with the infarct volume, which was higher in severe AIS patients.

Conclusion: Our study suggests that the increased α2δ-1 levels in serum and CSF specimens may be used as a potential marker for reflecting VGCCs dysfunction, illness severity and neuroinflammation in AIS patients.
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http://dx.doi.org/10.1016/j.jstrokecerebrovasdis.2021.105874DOI Listing
August 2021

Reconfigurable magnetic near-field distributions based on the coding metasurfaces in MHz band.

Opt Express 2021 Apr;29(9):13908-13924

Magnetic near-field control has attracted extensive attention and have a wide range of applications in filters, sensors, and energy-transfer devices. Coding metamaterial has the convenience of miniaturization and integration, which not only provides a bridge between physics and information science, but also exploits a whole new perspective for magnetic near-field control. In this work, we theoretically propose and experimentally verify that the coding metasurface can realize the reconfigurable magnetic near-field distributions. By adjusting the digital voltage signals which drive the resonant units of metasurface, capacitance of the resonator can be dynamically controlled, thus the dynamic modulation of magnetic near-field distribution can be achieved. Specially, this magnetic near-field control can be explained with the help of coupled mode theory and coherent superposition method. Moreover, simulated annealing algorithm is employed to determine working frequency of the whole modulation system, which can avoid time-consuming frequency scanning process. The experimental results are in good agreement with the calculated results. This work reveals the significant potential of coding metasurface for the experimental study of the magnetic near-field control and promotes the use of metasurface for numerous integrated functional devices.
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http://dx.doi.org/10.1364/OE.424234DOI Listing
April 2021

Actively controlled asymmetric edge states for directional wireless power transfer.

Opt Express 2021 Mar;29(5):7844-7857

Wireless power transfer (WPT) has triggered immense research interest in a range of practical applications, including mobile phones, logistic robots, medical-implanted devices and electric vehicles. With the development of WPT devices, efficient long-range and robust WPT is highly desirable but also challenging. In addition, it is also very important to actively control the transmission direction of long-range WPT. Recently, the rise of topological photonics provides a powerful tool for near-field robust control of WPT. Considering the technical requirements of robustness, long-range and directionality, in this work we design and fabricate a one-dimensional quasiperiodic Harper chain and realize the robust directional WPT using asymmetric topological edge states. Specially, by further introducing a power source into the system, we selectively light up two Chinese characters, which are composed of LED lamps at both ends of the chain, to intuitively show the long-range directional WPT. Moreover, by adding variable capacitance diodes into the topological quasiperiodic chain, we present an experimental demonstration of the actively controlled directional WPT based on electrically controllable coil resonators. With the increase in voltage, we measure the transmission at two ends of the chain and observe the change of transmission direction. The realization of an actively tuned topological edge states in the topological quasiperiodic chain will open up a new avenue in the dynamical control of robust long-range WPT.
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http://dx.doi.org/10.1364/OE.417887DOI Listing
March 2021

High-efficiency water-window x-ray generation from nanowire array targets irradiated with femtosecond laser pulses.

Opt Express 2021 Feb;29(4):5427-5436

We demonstrate the high-efficiency generation of water-window soft x-ray emissions from polyethylene nanowire array targets irradiated by femtosecond laser pulses at the intensity of 4×10 W/cm. The experimental results indicate more than one order of magnitude enhancement of the water-window x-ray emissions from the nanowire array targets compared to the planar targets. The highest energy conversion efficiency from laser to water-window x-rays is measured as 0.5%/sr, which comes from the targets with the longest nanowires. Supported by particle-in-cell simulations and atomic kinetic codes, the physics that leads to the high conversion efficiency is discussed.
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http://dx.doi.org/10.1364/OE.417512DOI Listing
February 2021

Controlled synthesis and upconversion luminescence properties of highly uniform and monodisperse β-NaYF :Yb ,Tm nanocrystals.

Luminescence 2021 Jun 2;36(4):1056-1062. Epub 2021 Mar 2.

School of Chemistry and Environmental Engineering, Changchun University of Science and Technology, Changchun, China.

Ytterbium, thulium (Yb ,Tm ) co-doped β-NaYF upconversion nanocrystals (U-NCs) were synthesized by a convenient hydrothermal method using sodium citrate as a capping agent. X-ray diffraction (XRD) analysis revealed that NCs phase structures were dependent on reaction time. Field-emission scanning electron microscopy (FE-SEM) and transmission electron microscopy (TEM) results showed that the morphology of U-NCs could be controlled by adjusting the concentration of sodium citrate added in the hydrothermal reaction. Moreover, the emission spectra of the U-NCs were investigated to evaluate upconversion efficiency. Strong luminescence intensity of the U-NCs was observed after tuned with optimized concentration of sodium citrate. Furthermore, the U-NCs were incubated with MCF-7 cells at 37°C for 24 h. Under irradiation of a 980 nm laser, the upconversion blue emission of the NCs in MCF-7 cells can be clearly observed through a confocal microscope with an upconversion imaging system and high quality upconversion luminescence images can be acquired. Thus, prepared β-NaYF :Yb ,Tm NCs provide us with highly efficient luminescent probes which can be applied for diverse bio-applications.
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http://dx.doi.org/10.1002/bio.4034DOI Listing
June 2021

A review on Cadmium Exposure in the Population and Intervention Strategies Against Cadmium Toxicity.

Bull Environ Contam Toxicol 2021 Jan 23;106(1):65-74. Epub 2021 Jan 23.

Key Laboratory of Environmental Medicine Engineering of Ministry of Education, School of Public Health, Southeast University, Nanjing, 210000, Jiangsu, China.

The rapid industrial development has led to serious cadmium (Cd) pollution. Cd is a toxic heavy metal placing severe health threat to human. Cd can enter the body through the atmosphere, water, soil and food, and has a long half-life (10-30 years), it largely accumulates in kidneys, liver, bone and other organs and causes irreversible damage to the target organs. Cd pollution has also further caused certain carcinogenic and non-carcinogenic health risk. This study summarizes the current situation of Cd pollution, the toxicity of specific target organs, carcinogenic risk and non-carcinogenic risk in the general population, as well as dietary supplements to prevent and mitigate Cd toxication, which aims to focus on the adverse effects of Cd to human from both individual and population perspectives, hoping that not only the health risk of Cd poisoning can be reduced, but also the accurate prevention and control of Cd poisoning can be achieved in the future.
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http://dx.doi.org/10.1007/s00128-020-03088-1DOI Listing
January 2021

Role of deubiquitinating enzymes in DNA double-strand break repair.

Authors:
Yunhui Li Jian Yuan

J Zhejiang Univ Sci B 2021 Jan;22(1):63-72

The Key Laboratory of Arrhythmias of the Ministry of Education of China, Research Center for Translational Medicine, East Hospital, Tongji University School of Medicine, Shanghai 200120, China.

DNA is the hereditary material in humans and almost all other organisms. It is essential for maintaining accurate transmission of genetic information. In the life cycle, DNA replication, cell division, or genome damage, including that caused by endogenous and exogenous agents, may cause DNA aberrations. Of all forms of DNA damage, DNA double-strand breaks (DSBs) are the most serious. If the repair function is defective, DNA damage may cause gene mutation, genome instability, and cell chromosome loss, which in turn can even lead to tumorigenesis. DNA damage can be repaired through multiple mechanisms. Homologous recombination (HR) and non-homologous end joining (NHEJ) are the two main repair mechanisms for DNA DSBs. Increasing amounts of evidence reveal that protein modifications play an essential role in DNA damage repair. Protein deubiquitination is a vital post-translational modification which removes ubiquitin molecules or polyubiquitinated chains from substrates in order to reverse the ubiquitination reaction. This review discusses the role of deubiquitinating enzymes (DUBs) in repairing DNA DSBs. Exploring the molecular mechanisms of DUB regulation in DSB repair will provide new insights to combat human diseases and develop novel therapeutic approaches.
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http://dx.doi.org/10.1631/jzus.B2000309DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7818009PMC
January 2021

AgNPs and MIL-101(Fe) self-assembled nanometer materials improved the SERS detection sensitivity and reproducibility.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Apr 31;251:119396. Epub 2020 Dec 31.

Chinese Academy of Inspection and Quarantine, Beijing 100123, China. Electronic address:

Recently, in the research of Surface-enhanced Raman scattering (SERS) technology, it is found that the preparation of enhanced substrate is particularly important. In this work, the most commonly used methods were used to synthesize AgNPs and MIL-101(Fe), and AgNPs/MIL-101(Fe) nanocomposite was obtained through self-assembly of the two substances. Four different probe molecules were detected with the self-assembled substrate and compared with the results of same probe molecules with AgNPs and MIL-101(Fe) as SERS substrate separately, it was found that AgNPs/ MIL-101 (Fe) nanocomposites had a strong enhancing effect as SERS substrate. The Enhancement Factor (EF) value of 10 mol/L Rhodamine 6G (R6G) was calculated as 2.09 × 10, and the Raman intensities of the peak relative standard deviation (RSD) of R6G Raman attribution was calculated as 7.55%. The time stability of the material was studied and it was found that the reduced Raman signal and poor reproducibility were due to the AgNPs placement time. AgNPs/ MIL-101 (Fe) nanocomposites were used as SERS substrate to detect Paraquat with a minimum concentration of 10 mol/L. The signal values of Paraquat Raman detected at 10 mol/L in different pH environments were relatively stable.
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http://dx.doi.org/10.1016/j.saa.2020.119396DOI Listing
April 2021

Classification of serous ovarian carcinoma based on immunogenomic profiling.

Int Immunopharmacol 2021 Feb 21;91:107274. Epub 2020 Dec 21.

Department of Gynecology, Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong Province, China. Electronic address:

Treatment of serous ovarian cancer (SOC) remains a clinical challenge. Classification of SOC based on immunogenomic profiling is important for establishing immunotherapy strategies. We extracted RNA-seq data of SOC from TCGA-OV. The samples were ultimately classified into high immune (Immunity_H) group and low immune (Immunity_L) group based on the immunogenomic profiling of 29 immune signatures by using unsupervised machine learning methods and modified by multifaceted characterization of immune response. High immune group showed the lower tumor purity and higher anti-tumor immune activity, and the higher expressions of PDCD1, CD274 and CTLA4. Furthermore, the overall survival time and the progression-free interval were significantly longer in high-immun group. The differentially expressed genes were mainly enriched in some immune response related functional terms and PI3K-AKT signaling pathway. According to ImmuCellAI, the abundance of various T cell subtypes in high immune group were significantly higher than those in low immune group. This novel immunotyping shows promise for prognostic and immunotherapeutic stratification in SOC patients.
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http://dx.doi.org/10.1016/j.intimp.2020.107274DOI Listing
February 2021

Serum-soluble ST2 as a novel biomarker reflecting inflammatory status and illness severity in patients with COVID-19.

Biomark Med 2020 12;14(17):1619-1629

Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Hubei, Wuhan, 430071, China.

The authors studied the role of soluble ST2 (sST2) in COVID-19 and its relationship with inflammatory status and disease severity. Serum levels of sST2 and interleukin (IL)-33, C-reactive protein (CRP), serum amyloid protein (SAA), IL-6 and procalcitonin (PCT), and T lymphocyte subsets from 80 subjects diagnosed with COVID-19 including 36 mild, 41 severe and three asymptomatic cases were tested. Serum sST2 levels were significantly increased in COVID-19 patients, which were positively correlated with CRP, but negatively correlated with CD4 and CD8 T lymphocyte counts. Serum sST2 levels in nonsurviving severe cases were persistently high during disease progression. Serum sST2 level test is helpful for reflecting inflammatory status and illness severity of COVID-19.
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http://dx.doi.org/10.2217/bmm-2020-0410DOI Listing
December 2020

In Situ Ligand-Transformation-Involved Synthesis of Inorganic-Organic Hybrid Polyoxovanadates as Efficient Heterogeneous Catalysts for the Selective Oxidation of Sulfides.

Inorg Chem 2020 Dec 10;59(23):17583-17590. Epub 2020 Nov 10.

Liaoning Professional Technology Innovation Center of Liaoning Province for Conversion Materials of Solar Cell, College of Chemistry and Materials Engineering, Bohai University, Jinzhou 121000, P. R. China.

By intentionally involving in situ ligand transformation in the reaction system, two inorganic-organic hybrid polyoxovanadates (POVs), [Co(HDTBA)VO] () and [Ni(HO)(DTBA)VO(OH)]·4HO (), have been synthesized by using a hydrothermal method, where the 3,5-di[1,2,4]triazol-1-ylbenzoic acid (HDTBA) ligand originated from in situ hydrolysis of 3,5-di[1,2,4]triazol-1-ylbenzonitrile in the self-assembly process. The inorganic layers [Co(VO)] containing [VO] circle clusters were linked by HDTBA ligands to yield a 3D framework structure of compound . There existed a kind of binuclear [(DTBA)VO(OH)] vanadium cluster grafted directly by two DTBA ligands through the sharing of carboxyl oxygen atoms in compound , further extended into a 2D layer by nickel centers. The investigations on the catalytic properties indicated that compounds and as heterogeneous catalysts, especially , owned satisfying catalytic performances for catalyzing the selective oxidation of sulfides to sulfoxides in the presence of -butyl hydroperoxide as an oxidant, accompanied by excellent conversion of 100% and selectivity of above 99%, providing a promising way for developing inorganic-organic hybrid POVs as effective heterogeneous catalysts for catalyzing the selective oxidation of sulfides.
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http://dx.doi.org/10.1021/acs.inorgchem.0c02798DOI Listing
December 2020

Effective optical nihility media realized by one-dimensional photonic crystals containing hyperbolic metamaterials.

Opt Express 2020 Oct;28(22):33198-33207

Owing to the omnidirectional perfect transmission and omnidirectional zero phase accumulation properties, S-type optical nihility media (ONM) have been utilized to design hyperlenses, optical waveguides, field concentrators and field rotators. Under the multiple interference mechanism, for conventional all-dielectric one-dimensional photonic crystals (1DPCs), all the transmittance peaks within the passband will shift towards short wavelengths (blueshift) with the increase in incident angle. Therefore, effective ONM cannot be realized in all-dielectric 1DPCs because the perfect transmission and zero phase accumulation conditions at the wavelength of the transmittance peak can only be satisfied at a specific incident angle. However, in a 1DPC composed of alternating dielectric and hyperbolic metamaterial (HMM) layers, one can realize a stopband of which one band edge is redshifted. At the same time, a transmittance peak in the passband is blueshifted. Therefore, between the redshift band edge and the blueshift transmittance peak, one can obtain an angle-independent transmittance peak. The HMM layer is mimicked by a dielectric/doped semiconductor multilayer. At the wavelength of the angle-independent transmittance peak, perfect transmission and zero phase accumulation conditions can be satisfied at any incident angle. Our work provides a route, under the current experimental conditions, to realize an effective S-type ONM by a simple one-dimensional structure in the near-infrared range.
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http://dx.doi.org/10.1364/OE.404998DOI Listing
October 2020

Identification of prognosis-related genes in the tumor microenvironment of stomach adenocarcinoma by TCGA and GEO datasets.

Biosci Rep 2020 10;40(10)

Clinical Laboratory, General Hospital of Northern Theatre Command, Shenyang 110003, China.

Accumulating evidence has demonstrated that tumor microenvironment (TME) plays a crucial role in stomach adenocarcinoma (STAD) development, progression, prognosis and immunotherapeutic responses. How the genes in TME interact and behave is extremely crucial for tumor investigation. In the present study, we used gene expression data of STAD available from TCGA and GEO datasets to infer tumor purity using ESTIMATE algorithms, and predicted the associations between tumor purity and clinical features and clinical outcomes. Next, we calculated the differentially expressed genes (DEGs) from the comparisons of immune and stromal scores, and postulated key biological processes and pathways that the DEGs mainly involved in. Then, we analyzed the prognostic values of DEGs in TCGA dataset, and validated the results by GEO dataset. Finally, we used CIBERSORT computational algorithm to estimate the 22 tumor infiltrating immune cells (TIICs) subsets in STAD tissues. We found that stromal and immune scores were significantly correlated with STAD subtypes, clinical stages, Helicobacter polyri infection, and stromal scores could predict the clinical outcomes in STAD patients. Moreover, we screened 307 common DEGs in TCGA and GSE51105 datasets. In the prognosis analyses, we only found OGN, JAM2, RERG, OLFML2B, and ADAMTS1 genes were significantly associated with overall survival in TCGA and GSE84437 datasets, and these genes were correlated with the fractions of T cells, B cells, macrophages, monocytes, NK cells and DC cells, respectively. Our comprehensive analyses for transcriptional data not only improved the understanding of characteristics of TME, but also provided the targets for individual therapy in STAD.
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http://dx.doi.org/10.1042/BSR20200980DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7560520PMC
October 2020

Potential in paleoclimate reconstruction of modern pollen assemblages from natural and human-induced vegetation along the Heilongjiang River basin, NE China.

Sci Total Environ 2020 Nov 22;745:141121. Epub 2020 Jul 22.

Key Laboratory of Wetland Ecology and Environment, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China. Electronic address:

The relationships among modern pollen, vegetation, climate and human activities can help improving the reliability of reconstruction of past vegetation, regional climate and human activities based on fossil pollen records. We used a dataset of 114 surface soil pollen samples from natural vegetation (wetlands, forests and grasslands) and human-induced vegetation (farmlands and residences) along the Heilongjiang River basin in northeast China to explore the relationships among modern pollen, vegetation, climate and human activities. The results indicated that surface pollen assemblages differentiated modern vegetation well in natural and human-induced vegetation types. The wetlands were mainly composed of Cyperaceae, along with Artemisia, weeds Poaceae (<35 μm) and Sanguisorba. The forests were predominated by Pinus and Betula. Artemisia, weeds Poaceae (<35 μm) and Chenopodiaceae were the most important pollen taxa in grasslands. The farmlands were characterized by Artemisia, Aster, Chenopodiaceae, cereal Poaceae (>35 μm) and Taraxacum. The pollen assemblages of residences were composed of weeds Poaceae (<35 μm), Chenopodiaceae and Salix. Ordination analyses based on main pollen taxa and climatic variables were used to determine the relationships between pollen and climate, suggesting the surface pollen assemblages were primarily influenced by the mean annual temperature (Tann) in northeast China. The statistical performance of transfer function between pollen and Tann were well indicating the modern pollen assemblages could be reliably used in paleoclimate reconstruction in our study area. Furthermore, human-induced vegetation had high frequencies of human-companion pollen taxa, such as Chenopodiaceae, Aster, Taraxacum and cereal Poaceae (>35 μm). Pollen concentrations of human-induced vegetation were lower than natural vegetation types, which could be used as an indicator of human influence intensity.
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http://dx.doi.org/10.1016/j.scitotenv.2020.141121DOI Listing
November 2020

Comprehensive Analysis of Prognostic Alternative Splicing Signatures in Endometrial Cancer.

Front Genet 2020 29;11:456. Epub 2020 May 29.

Department of Gynecology, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China.

Background: Alternative splicing (AS) is one of the critical post-transcriptional regulatory mechanisms of various cancers and also plays a crucial role in the development of cancers, including endometrial cancer (EC).

Methods: The splicing data and gene expression profiles of EC were obtained from The Cancer Genome Atlas. The corresponding clinical data were extracted from TCGA-CDR. With univariate Cox regression analysis, least absolute shrinkage and selection operator model, and multivariate Cox regression analysis, the survival-related AS events were selected. Functional enrichment analysis was also performed to investigate the functions of these AS events. Splicing factors and AS regulation network were constructed to understand the correlation among these AS events.

Result: A total of 1826 AS events were identified as survival-related events. Functional enrichment analysis showed that these AS events were associated with several immune system-related processes. Then, the prognostic signatures were developed based on these survival-related events and acted as an independent prognostic factor for EC. Splicing factors and AS regulation network were also constructed to understand the regulatory mechanisms of AS events in EC.

Conclusion: This study systematically analyzed the role of AS events in EC and developed the prognostic model for EC.
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http://dx.doi.org/10.3389/fgene.2020.00456DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7272712PMC
May 2020

Intestinal mir-794 responds to nanopolystyrene by linking insulin and p38 MAPK signaling pathways in nematode Caenorhabditis elegans.

Ecotoxicol Environ Saf 2020 Sep 10;201:110857. Epub 2020 Jun 10.

Key Laboratory of Environmental Medicine Engineering in Ministry of Education, Medical School, Southeast University, Nanjing, 210009, China; Shenzhen Ruipuxun Academy for Stem Cell & Regenerative Medicine, Shenzhen, 518122, China; Guangdong Provincial Key Laboratory of Environmental Pollution and Health, School of Environment, Jinan University, Guangzhou, 510632, China. Electronic address:

Caenorhabditis elegans is sensitive to toxicity of environmental pollutants. The alteration in expression of mir-794, a microRNA (miRNA) molecule, mediated a protective response to nanopolystyene (100 nm) at predicted environmental concentration (1 μg/L) in nematodes. However, the underlying molecular basis for mir-794 function in regulating the response to nanopolystyrene remains largely unclear. In this study, we found that intestinal overexpression of mir-794 caused the susceptibility to nanopolystyrene toxicity, suggesting that mir-794 acted in the intestine to regulate the response to nanopolystyrene. Intestinal overexpression of mir-794 further decreased the expressions of daf-16 encoding a FOXO transcriptional factor in insulin signaling pathway, skn-1 encoding a Nrf transcriptional factor in p38 MAPK signaling pathway, and mdt-15 encoding a lipid metabolic sensor acting downstream of SKN-1 in nanopolystyrene exposed nematodes. Meanwhile, intestinal overexpression of mir-794 could suppress the resistance of nematodes overexpressing intestinal daf-16, skn-1, or mdt-15 containing the corresponding 3' untranslated region (3' UTR) to nanopolystyrene toxicity. Therefore, DAF-16, SKN-1, and MDT-15 acted as the downstream targets of intestinal mir-794 to regulate the response to nanopolystyrene. In the intestine, DAF-16 functioned synergistically with SKN-1 or MDT-15 to regulate the response to nanopolystyrene. Our results suggested that the intestinal mir-794 provided an important epigenetic regulation mechanism to control the response to nanopolystyrene by linking insulin and p38 MAPK signaling pathways in nematodes.
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http://dx.doi.org/10.1016/j.ecoenv.2020.110857DOI Listing
September 2020

Comprehensive analysis of microenvironment-related genes in lung adenocarcinoma.

Future Oncol 2020 Aug 5;16(24):1825-1837. Epub 2020 Jun 5.

Bioinformatics of Department, Key laboratory of Cell Biology, Ministry of Public Health, & Key Laboratory of Medical Cell Biology, Ministry of Education, School of Life Sciences, China Medical University, Shenyang 110122, PR China.

Understanding the cell types and cell compositions in tumor environment (TME) and the gene changes in lung adenocarcinoma (LUAD) may provide insights on immune profiles and treatment targets for LUAD patients. The RNA-Seq data from The Cancer Genome Atlas database were used to calculate the stromal scores and immune scores and analyzed the fractions of tumor infiltrating immune cells in LUAD samples with ESTIMATE and CIBERSORT algorithm. We extracted a list of TME-related differentially expressed genes and performed the functional enrichment analysis. We found these genes were mainly enriched in immune response and cancer-related signal pathways. The prognosis analysis indicated that , , , and were associated with prognosis in LUAD patients. The current study provides novel insights into immune files and gene changes in TME in LUAD patients.
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http://dx.doi.org/10.2217/fon-2019-0829DOI Listing
August 2020

Controllable Carbonization of Plastic Waste into Three-Dimensional Porous Carbon Nanosheets by Combined Catalyst for High Performance Capacitor.

Nanomaterials (Basel) 2020 Jun 2;10(6). Epub 2020 Jun 2.

Faculty of Chemical Technology and Engineering, West Pomeranian University of Technology, Piastow Ave. 42, 71-065 Szczecin, Poland.

Polyethylene terephthalate (PET) plastic has been extensively used in our social life, but its poor biodegradability has led to serious environmental pollution and aroused worldwide concern. Up to now, various strategies have been proposed to address the issue, yet such strategies remain seriously impeded by many obstacles. Herein, waste PET plastic was selectively carbonized into three-dimensional (3D) porous carbon nanosheets (PCS) with high yield of 36.4 wt%, to be further hybridized with MnO nanoflakes to form PCS-MnO composites. Due to the introduction of an appropriate amount of MnO nanoflakes, the resulting PCS-MnO composite exhibited a specific capacitance of 210.5 F g as well as a high areal capacitance of 0.33 F m. Furthermore, the PCS-MnO composite also showed excellent cycle stability (90.1% capacitance retention over 5000 cycles under a current density of 10 A g). The present study paved an avenue for the highly efficient recycling of PET waste into high value-added products (PCSs) for electrochemical energy storage.
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http://dx.doi.org/10.3390/nano10061097DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7353313PMC
June 2020

Effect of chronic exposure to nanopolystyrene on nematode Caenorhabditis elegans.

Chemosphere 2020 Oct 24;256:127172. Epub 2020 May 24.

Key Laboratory of Environmental Medicine Engineering of Ministry of Education, Medical School, Southeast University, Nanjing, 210009, China; Shenzhen Ruipuxun Academy for Stem Cell & Regenerative Medicine, Shenzhen, 518122, China. Electronic address:

Nanoplastic exposure could cause toxicity to Caenorhabditis elegans at various aspects. Nevertheless, the effects of chronic exposure to nanoplastics remain largely unclear in nematodes. In this study, we employed C. elegans as an animal model to determine the effects of nanopolystyrene (30 nm) exposure from adult day-1 for 8-day. After the exposure, only 1000 μg/L nanopolystyrene reduced the lifespan. In contrast, nanopolystyrene ≥1 μg/L decreased locomotion behavior and activated oxidative stress. Meanwhile, in 10 μg/L nanopolystyrene exposed nematodes, both expression of SOD-3, a Mn-SOD, and autophagy induction as indicated by LGG-1:GFP expression were significantly increased. RNAi knockdown of daf-2 encoding an insulin receptor enhanced the autophagy induction, and RNAi knockdown of daf-16 encoding a FOXO transcriptional factor in insulin signaling pathway suppressed the autophagy induction in 10 μg/L nanopolystyrene exposed nematodes. Moreover, DAF-16 acted upstream of LGG-1, an ortholog of Atg8/LC3, to regulate the toxicity of nanopolystyrene toxicity in inducing ROS production and in decreasing locomotion behavior at adult day-9. Our data implied the potential toxicity of chronic exposure to nanoplastics at predicted environmental concentrations on organisms.
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http://dx.doi.org/10.1016/j.chemosphere.2020.127172DOI Listing
October 2020

Burnout in pediatric hematology oncology fellows: Results of a cross-sectional survey.

Pediatr Blood Cancer 2020 11 11;67(11):e28274. Epub 2020 Apr 11.

Dana-Farber/Boston Children's Cancer and Blood Disorders Center, Boston, Massachusetts.

Burnout is a significant problem in the medical community, including among pediatric hematology/oncology (PHO) faculty. However, the prevalence of burnout, its associated risk factors, and outcomes within PHO fellows are unknown. We present the results of a cross-sectional study of PHO fellows from 21 training programs. A total of 45/115 fellows (39.1%) met criteria for high level of burnout. Fellows who met criteria for high burnout also demonstrated poor outcomes including decreased empowerment, increased doctor-centered care, decreased self-assessed humanism, and decreased satisfaction with training. Further longitudinal investigation is needed to better understand burnout and the causative factors affecting PHO fellows.
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http://dx.doi.org/10.1002/pbc.28274DOI Listing
November 2020

Fouling characterization and aeration performance recovery of fine-pore diffusers operated for 10 years in a full-scale wastewater treatment plant.

Bioresour Technol 2020 Jul 17;307:123197. Epub 2020 Mar 17.

Shanghai Chengtou Wastewater Treatment Co., Ltd, Shanghai 201203, China.

Fouling characterization and aeration performance recovery of fine-pore diffusers operated for 10 years in a full-scale wastewater treatment plant were investigated to elucidate fouling mechanisms and develop cleaning strategy. The performance decline of diffusers was observed with dynamic wet pressure increased by 3.2 times and standard oxygen transfer efficiency dropped to 73%, which contributed to 15.0% increase in total energy consumption. Oxygen-affinity, filamentous and extracellular polymeric substances secreting bacteria tended to accumulate on the diffuser surface. External (mainly biofilm growth), internal (organic and inorganic matters) and irrecoverable (mainly material aging) foulants accounted for 34.1%, 45.4% and 20.1% of total fouling, respectively. HCl cleaning failed to restore aeration efficiency because it eliminated structural support formed by inorganics, leaving organic foulants broken into smaller fragments and distributed more dispersed. NaClO showed better cleaning efficiency by effectively removing organic foulants. Sequential cleaning by NaClO and HCl, which achieved the best recovery, was recommended.
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http://dx.doi.org/10.1016/j.biortech.2020.123197DOI Listing
July 2020

Stability of the permafrost peatlands carbon pool under climate change and wildfires during the last 150 years in the northern Great Khingan Mountains, China.

Sci Total Environ 2020 Apr 7;712:136476. Epub 2020 Jan 7.

Key Laboratory of Wetland Ecology and Environment, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Shengbei Street 4888, 130102 Changchun, China. Electronic address:

Peatlands store one-third of the total global soil carbon (C.) despite covering only 3-4% of the global land surface. Most peatlands are distributed in mid-high latitude regions and are even in permafrost regions, are sensitive to climate change and are disturbed by wildfire. Although several studies have focused on the impact of historical climate change and regional human activities on the C. accumulation process in these peatlands, the impact of these factors on the stability of the C. pool remains poorly understood. Here, based on the Pb age-depth model, we investigated the historical variations of C. stability during the last 150 years for five typical peatlands in the northern Great Khingan Mountains (Northeast China), an area located in a permafrost region that is sensitive to climate change and to wildfires, which have clearly increased due to regional human activities. The results showed that low C. accumulation rates (CARs) and weakly C. stability in studied peatlands before 1900. While, the increasing anthropogenic wildfire frequency and the residual products (e.g. pyrogenic carbon) increased the CARs and C. stability in peatlands from 1900 to 1980. The mean July temperature is the most important climate factor for peatlands C. stability. After 1980, due to the low wildfire frequencies influenced by human policies, increasing temperatures and decreasing precipitation not only increased the CARs but also markedly increased the C. stability of the peatlands C. pool in the northern Great Khingan Mountains, especially after 2000.
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http://dx.doi.org/10.1016/j.scitotenv.2019.136476DOI Listing
April 2020

CHK2-FOXK axis promotes transcriptional control of autophagy programs.

Sci Adv 2020 01 1;6(1):eaax5819. Epub 2020 Jan 1.

Research Center for Translational Medicine, East Hospital, Tongji University School of Medicine, Shanghai 200120, China.

Autophagy is an evolutionarily conserved catabolic process, which plays a vital role in removing misfolded proteins and clearing damaged organelles to maintain internal environment homeostasis. Here, we uncovered the checkpoint kinase 2 (CHK2)-FOXK (FOXK1 and FOXK2) axis playing an important role in DNA damage-mediated autophagy at the transcriptional regulation layer. Mechanistically, following DNA damage, CHK2 phosphorylates FOXK and creates a 14-3-3γ binding site, which, in turn, traps FOXK proteins in the cytoplasm. Because FOXK functions as the transcription suppressor of ATGs, DNA damage-mediated FOXKs' cytoplasmic trapping induces autophagy. In addition, we found that a cancer-derived FOXK mutation induces FOXK hyperphosphorylation and enhances autophagy, resulting in chemoresistance. Cotreatment with cisplatin and chloroquine overcomes the chemoresistance caused by FOXK mutation. Overall, our study highlights a mechanism whereby DNA damage triggers autophagy by increasing autophagy genes via CHK2-FOXK-mediated transcriptional control, and misregulation of this pathway contributes to chemoresistance.
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http://dx.doi.org/10.1126/sciadv.aax5819DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6938702PMC
January 2020

Potential toxicity of nanopolystyrene on lifespan and aging process of nematode Caenorhabditis elegans.

Sci Total Environ 2020 Feb 5;705:135918. Epub 2019 Dec 5.

Medical School, Southeast University, Nanjing 210009, China. Electronic address:

In the environment, nanoplastic particles, such as nanopolystyrene, potentially cause toxicity on organisms at various aspects. We here employed endpoints of lifespan and aging-related phenotypes to further investigate the possible long-term effects of nanopolystyrene (100 nm) in Caenorhabditis elegans. After exposure from L1-larvae to adult day-3, nanopolystyrene at high concentrations (100 and 1000 μg/L) reduced the lifespan. Although nanopolystyrene (1 or 10 μg/L) did not affect the lifespan, nanopolystyrene (1 or 10 μg/L) could induce the more severe intestinal reactive oxygen species (ROS) production and decrease in locomotion behavior during the aging process compared with control. Moreover, nanopolystyrene exposure could cause the severe decrease in expressions of some immune response genes, hsp-6 gene, and genes encoding manganese-superoxide dismutases (Mn-SODs) during aging process, suggesting the severe suppression in innate immune response, inhibition in antioxidation defense system, and suppression in mitochondrial unfolded protein response (mt UPR) by nanopolystyrene. Our results highlight the potential of long-term nanopolystyrene exposure in reducing longevity and in affecting health state during the aging process in environmental organisms.
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http://dx.doi.org/10.1016/j.scitotenv.2019.135918DOI Listing
February 2020

Controlling the spin-selective absorption with two-dimensional chiral plasmonic gratings.

Opt Lett 2019 Dec;44(23):5868-5871

Spin-selective absorption in a two-dimensional (2D) chiral plasmonic grating is observed by excitation of chiral-dependent plasmonic cavity resonance. For the proposed structures, the incident right-handed circularly polarized light is absorbed with nearly 100% efficiency, whereas the incident left-handed circularly polarized light is reflected with same handedness. Moreover, we show that the location of spin-selective absorption can be controlled flexibly by tuning the plasmonic cavity dimension. The intensity of spin-selective absorption can be enhanced as well as suppressed based on Fabry-Perot interference phase relation. Such 2D chiral plasmonic gratings could find many potential applications in novel photon-spin selective devices, such as circularly polarized light detectors/emitters, chiral sensors, chiral cavities, and spin lasers.
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http://dx.doi.org/10.1364/OL.44.005868DOI Listing
December 2019
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